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https://www.readbyqxmd.com/read/27914607/ultra-high-performance-size-exclusion-chromatography-in-polar-solvents
#1
Gertjan Vancoillie, Maarten Vergaelen, Richard Hoogenboom
Size-exclusion chromatography (SEC) is amongst the most widely used polymer characterization methods in both academic and industrial polymer research allowing the determination of molecular weight and distribution parameters, i.e. the dispersity (Ɖ), of unknown polymers. The many advantages, including accuracy, reproducibility and low sample consumption, have contributed to the worldwide success of this analytical technique. The current generation of SEC systems have a stationary phase mostly containing highly porous, styrene-divinylbenzene particles allowing for a size-based separation of various polymers in solution but limiting the flow rate and solvent compatibility...
November 21, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27910942/improved-bi-allelic-modification-of-a-transcriptionally-silent-locus-in-patient-derived-ipsc-by-cas9-nickase
#2
Reto Eggenschwiler, Mohsen Moslem, Mariane Serra Fráguas, Melanie Galla, Oliver Papp, Maximilian Naujock, Ines Fonfara, Ingrid Gensch, Annabell Wähner, Abbas Beh-Pajooh, Claudio Mussolino, Marcel Tauscher, Doris Steinemann, Florian Wegner, Susanne Petri, Axel Schambach, Emmanuelle Charpentier, Toni Cathomen, Tobias Cantz
Homology directed repair (HDR)-based genome editing via selectable long flanking arm donors can be hampered by local transgene silencing at transcriptionally silent loci. Here, we report efficient bi-allelic modification of a silent locus in patient-derived hiPSC by using Cas9 nickase and a silencing-resistant donor construct that contains an excisable selection/counter-selection cassette. To identify the most active single guide RNA (sgRNA)/nickase combinations, we employed a lentiviral vector-based reporter assay to determine the HDR efficiencies in cella...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27909033/ethnic-differences-in-the-prevalence-of-knee-pain-among-adults-of-a-community-in-a-cross-sectional-study
#3
Yook Chin Chia, Hooi Chin Beh, Chirk Jenn Ng, Cheong Lieng Teng, Nik Sherina Hanafi, Wan Yuen Choo, Siew Mooi Ching
OBJECTIVE: To determine the prevalence of knee pain among 3 major ethnic groups in Malaysia. By identifying high-risk groups, preventive measures can be targeted at these populations. DESIGN AND SETTING: A cross-sectional survey was carried out in rural and urban areas in a state in Malaysia. Secondary schools were randomly selected and used as sampling units. PARTICIPANTS: Adults aged ≥18 years old were invited to answer a self-administered questionnaire on pain experienced over the previous 6 months...
December 1, 2016: BMJ Open
https://www.readbyqxmd.com/read/27907855/lc-ms-ms-assay-for-the-quantitation-of-the-tyrosine-kinase-inhibitor-neratinib-in-human-plasma
#4
Brian F Kiesel, Robert A Parise, Alvin Wong, Kiana Keyvanjah, Samuel Jacobs, Jan H Beumer
Neratinib is an orally available tyrosine kinase inhibitor targeting HER2 (ERBB2) and EGFR (ERBB). It is being clinically evaluated for the treatment of breast and other solid tumors types as a single agent or in combination with other chemotherapies. In support of several phase I/II clinical trials investigating neratinib combinations, we developed and validated a novel LC-MS/MS assay for the quantification of neratinib in 100μL of human plasma with a stable isotopic internal standard. Analytes were extracted from plasma using protein precipitation and evaporation of the resulting supernatant followed by resuspension...
November 22, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/27898310/potential-human-exposure-to-halogenated-flame-retardants-in-elevated-surface-dust-and-floor-dust-in-an-academic-environment
#5
Jaime M Allgood, Tamara Jimah, Carolyn M McClaskey, Mark J La Guardia, Stephanie C Hammel, Maryam M Zeineddine, Ian W Tang, Miryha G Runnerstrom, Oladele A Ogunseitan
Most households and workplaces all over the world possess furnishings and electronics, all of which contain potentially toxic flame retardant chemicals to prevent fire hazards. Indoor dust is a recognized repository of these types of chemicals including polybrominated diphenyl ethers (PBDEs) and non-polybrominated diphenyl ethers (non-PBDEs). However, no previous U.S. studies have differentiated concentrations from elevated surface dust (ESD) and floor dust (FD) within and across microenvironments. We address this information gap by measuring twenty-two flame-retardant chemicals in dust on elevated surfaces (ESD; n=10) and floors (FD; n=10) from rooms on a California campus that contain various concentrations of electronic products...
November 26, 2016: Environmental Research
https://www.readbyqxmd.com/read/27895593/baseline-muscle-mass-is-a-poor-predictor-of-functional-overload-induced-gain-in-the-mouse-model
#6
Audrius Kilikevicius, Lutz Bunger, Arimantas Lionikas
Genetic background contributes substantially to individual variability in muscle mass. Muscle hypertrophy in response to resistance training can also vary extensively. However, it is less clear if muscle mass at baseline is predictive of the hypertrophic response. The aim of this study was to examine the effect of genetic background on variability in muscle mass at baseline and in the adaptive response of the mouse fast- and slow-twitch muscles to overload. Males of eight laboratory mouse strains: C57BL/6J (B6, n = 17), BALB/cByJ (n = 7), DBA/2J (D2, n = 12), B6...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27885942/enlarged-subarachnoid-spaces-and-intracranial-hemorrhage-in-children-with-accidental-head-trauma
#7
Amanda K Fingarson, Maura E Ryan, Suzanne G McLone, Corey Bregman, Emalee G Flaherty
OBJECTIVE Benign external hydrocephalus (BEH) is an enlargement of the subarachnoid spaces (SASs) that can be seen in young children. It is controversial whether children with BEH are predisposed to developing subdural hemorrhage (SDH) with or without trauma. This issue is clinically relevant as a finding of unexplained SDH raises concerns about child abuse and often prompts child protection and law enforcement investigations. METHODS This retrospective study included children (1-24 months of age) who underwent head CT scanning after an accidental fall of less than 6 feet...
November 25, 2016: Journal of Neurosurgery. Pediatrics
https://www.readbyqxmd.com/read/27884470/the-first-exposure-assessment-of-legacy-and-unrestricted-brominated-flame-retardants-in-predatory-birds-of-pakistan
#8
Naeem Akhtar Abbasi, Igor Eulaers, Veerle Leontina Bernard Jaspers, Muhammad Jamshed Iqbal Chaudhry, Adrien Frantz, Per Lennart Ambus, Adrian Covaci, Riffat Naseem Malik
The exposure to legacy polybrominated diphenyl ethers (PBDEs), hexabromocyclododecanes (HBCDDs) and unrestricted 1,2-bis (2,4,6-tribromophenoxy) ethane (BTBPE), bis (2-ethylhexyl)-2,3,4,5-tetrabromophthalate (BEH-TEBP) and 2-ethylhexyl-2,3,4,5-tetrabromo-benzoate (EH-TBB) was examined in tail feathers of 76 birds belonging to ten predatory species inhabiting Pakistan. In addition, different feather types of six individuals of Black kite (Milvus migrans) were compared for their brominated flame retardant (BFR) levels...
January 2017: Environmental Pollution
https://www.readbyqxmd.com/read/27883144/robust-synthesis-of-f-bodipys
#9
Michael Helmut Reynolds Beh, Katherine Isabelle Biehn Douglas, Kelsey Taylor Elver House, Alexandra Celeste Murphy, Jackson Scott Testemale Sinclair, Alison Thompson
A protocol is established for the high-yielding synthesis of F-BODIPYs involving non-anhydrous reagents and not requiring precautions to exclude moisture. This simple and robust strategy simply requires a second addition of NEt3 and BF3·OEt2, midway through the reaction period. The ratio and amounts of NEt3 and BF3·OEt2 used in each aliquot are critical to success (6 : 9 for each aliquot). The protocol can be completed using bench-dry apparatus, without need to achieve and maintain anhydrous conditions or solvents...
November 24, 2016: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/27867116/automatic-and-rapid-identification-of-glycopeptides-by-nano-uplc-ltq-ft-ms-and-proteomic-search-engine
#10
Estela Giménez, Marina Gay, Marta Vilaseca
: Here we demonstrate the potential of nano-UPLC-LTQ-FT-MS and the Byonic™ proteomic search engine for the separation, detection, and identification of N- and O-glycopeptide glycoforms in standard glycoproteins. The use of a BEH C18 nanoACQUITY column allowed the separation of the glycopeptides present in the glycoprotein digest and a baseline-resolution of the glycoforms of the same glycopeptide on the basis of the number of sialic acids. Moreover, we evaluated several acquisition strategies in order to improve the detection and characterization of glycopeptide glycoforms with the maximum number of identification percentages...
November 17, 2016: Journal of Proteomics
https://www.readbyqxmd.com/read/27859537/development-of-a-targeted-method-for-quantification-of-gypenoside-xlix-in-rat-plasma-using-spe-and-lc-ms-ms
#11
Song Guo, Chengxu Sui, Ying Ma
A sensitive, selective and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of gypenoside XLIX, a naturally occurring gypenoside of Gynostemma pentaphyllum in rat plasma and then validated according to FDA Guidance for industry-bioanalytical method validation. Plasma samples were prepared by a simple solid-phase extraction. Separation was performed on a Waters XBridgeTM BEH C18 chromatography column (4.6 mm × 50 mm, 2.5 µm) using a mobile phase of acetonitrile and water (62...
November 16, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27851687/determination-of-antidepressants-in-hair-via-uhplc-ms-ms-as-a-complementary-informative-tool-for-clinical-and-forensic-toxicological-assessments
#12
María Del Mar Ramírez Fernández, Sarah M R Wille, Virginia Hill, Nele Samyn
BACKGROUND: Hair analysis is a complementary approach for the detection of antidepressants (ADs) in clinical and forensic schemes because it yields a picture of long-term exposure over a time window depending on the length of the hair. METHODS: A fast and sensitive ultra-high performance liquid chromatography tandem mass spectrometry method using a BEH C18 column with a mobile phase consisting of ammonium acetate/acetonitrile was developed and validated according to international guidelines for the simultaneous analysis of 24 ADs in hair...
December 2016: Therapeutic Drug Monitoring
https://www.readbyqxmd.com/read/27848005/neurocognitive-and-psychosocial-function-in-children-with-benign-external-hydrocephalus-beh-a-long-term-follow-up-study
#13
Rebecka Mikkelsen, Linn Nilsen Rødevand, Ulrikke Straume Wiig, Sverre Morten Zahl, Torhild Berntsen, Anne-Britt Skarbø, Arild Egge, Eirik Helseth, Stein Andersson, Knut Wester
INTRODUCTION: Long-term impact of benign external hydrocephalus (BEH) on cognition is largely unknown, and indication for neurosurgical CSF diversion procedure is debated. This study reports neuropsychological and psychosocial function in operated and non-operated BEH children. METHODS: Eighty-six children (76 males) between 8 and 18 years (mean 13.9) diagnosed with BEH before 12 months were included, of whom 30.2 % were operated. Participants completed neuropsychological tests and questionnaires covering quality of life (PedsQL) and executive function (BRIEF)...
November 15, 2016: Child's Nervous System: ChNS: Official Journal of the International Society for Pediatric Neurosurgery
https://www.readbyqxmd.com/read/27773200/hyperdensity-of-the-basilar-artery-on-post-mortem-ct-an-indication-for-basilar-artery-thrombosis
#14
J Garland, R Tse, R Beh, T J Lyons, A D Cala
No abstract text is available yet for this article.
February 2016: Pathology
https://www.readbyqxmd.com/read/27772993/defending-cases
#15
Philip Beh
No abstract text is available yet for this article.
February 2016: Pathology
https://www.readbyqxmd.com/read/27772989/pathologists-as-communicators
#16
Philip Beh
No abstract text is available yet for this article.
February 2016: Pathology
https://www.readbyqxmd.com/read/27772986/the-coronial-autopsy-where-are-we-heading
#17
Neil Langlois, Helen McKelvie, Philip Beh, Simon Stables, Matthew Lynch, Noel Woodford, David Ranson, John Drayton, Jane Mowll, Chris O'Donnell, Sarah Parsons
No abstract text is available yet for this article.
February 2016: Pathology
https://www.readbyqxmd.com/read/27771258/development-and-validation-of-a-sensitive-and-selective-lc-ms-ms-method-for-determination-of-tacrolimus-in-oral-fluids
#18
Mwlod Ghareeb, Fatemeh Akhlaghi
Tacrolimus is a commonly used immunosuppressive agent in organ transplant recipients. Therapeutic drug monitoring (TDM) of tacrolimus is essential to adjust the dose and achieve optimal immunosuppression level. Routine TDM is practiced using whole blood samples obtained through venipuncture. However, tacrolimus concentration that is present in oral fluid (OF) can theoretically represent the free or pharmacologically active form of tacrolimus. In this study, we report the development and validation of a rapid, sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantification of tacrolimus in OF...
October 11, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27765135/-comparison-of-gender-specific-structure-profiles-of-immunoglobulin-g-n-glycans
#19
Y Sun, Y X Wang, J Zhang, X W Yu, S S Ge, H Wang, J Dong, X H Guo, W Wang
Objective: To analyze and compare the gender specific structure profiles of immunoglobulin G(IgG) N-glycans and provide evidence to understand the distribution of the structure of IgG N-glycans in general population. Methods: A cluster sampling was conducted to randomly select 669 participants from Beijing Xuanwu Hospital during January-June 2012. Venous blood sample (5 ml) was collected from each participant with vacuum negative pressure tube containing EDTA. Plasma IgG N-glycome was determined using ultra-performance liquid chromatography (UPLC) on Waters BEH Glycan chromatography column and mass spectrometry after the separation, purification and labeling...
October 10, 2016: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/27761186/paediatric-post-traumatic-osseous-cystic-lesion-following-a-distal-radial-fracture
#20
Joey Chan Yiing Beh, Ehab Shaban Mahmoud Hamouda
Post-traumatic osseous cystic lesions are a rare complication in children. An aetiology of intramedullary fat seepage through the damaged bone cortex and its entrapment within the subperiosteum has been proposed. These lesions run a benign course and usually resolve spontaneously. The presence of fatty marrow gives it a distinct appearance which aids in its diagnosis and differentiation from other bone lesions. This case demonstrates a fat-fluid level within the subperiosteal cystic lesion in Magnetic Resonance Imaging (MRI) and this is a typical feature of post-traumatic cystic lesion in a child...
July 2016: Journal of Radiology Case Reports
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