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https://www.readbyqxmd.com/read/28944677/a-multiplex-and-label-free-relative-quantification-approach-for-studying-protein-abundance-of-drug-metabolizing-enzymes-in-human-liver-microsomes-using-swath-ms
#1
Rohitash Jamwal, Benjamin J Barlock, Sravani Adusumalli, Ken Ogasawara, Brigitte L Simons, Fatemeh Akhlaghi
We describe a sequential windowed acquisition of all theoretical fragment ion mass spectra (SWATH-MS) based method for label-free, simultaneous, relative quantification of drug metabolism enzymes in human liver microsomes (HLM; n=78). In-solution tryptic digestion was aided by a pressure cycling method which allowed a 90-min incubation time, a significant reduction over classical protocols (12-18 h). Digested peptides were separated on an Acquity UHPLC Peptide BEH C18 column using a 60-min gradient method at a flow rate of 0...
September 25, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28934681/simultaneous-determination-of-gentiopicroside-and-its-two-active-metabolites-in-rat-plasma-by-lc-ms-ms-and-its-application-in-pharmacokinetic-studies
#2
Kai Xiong, Tingting Gao, Tong Zhang, Zhengtao Wang, Han Han
Gentiopicroside is a natural secoiridoid glycoside that may require metabolic activation to exert pharmacological effects. In this study, two active metabolites of gentiopicroside (M1 and M2) were isolated from rat urines and identified with our previous method. Most importantly, a fast, sensitive and selective ultra high-performance liquid chromatography-tandem mass spectrometry method was developed to simultaneously determine gentiopicroside and its two metabolites in rat plasma. The analytes and internal standard (swertiamarin) were separated on an ACQUITY UPLC(®) BEH C18 column (2...
September 14, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28934031/simultaneous-detection-of-15-antibiotic-growth-promoters-in-bovine-muscle%C3%AF-blood-and-urine-by-uplc-ms-ms
#3
Zhi Wang, Zongwei Shi, Cunxian Xi, Guomin Wang, Shurui Cao, Lei Zhang, Bobin Tang, Zhaode Mu
An analytical method was established for the rapid detection of antibiotic growth promoters (AGPs) in bovine muscle, and bovine blood and bovine urine, using ultra high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). After the addition of an aqueous solution of EDTA-Na2, the pH of bovine urine samples was directly adjusted to 5.2 by acetic acid-ammonium acetate and purified by HLB solid-phase extraction cartridge; bovine muscle and bovine blood samples processing were extracted with acetonitrile (ACN) and ACN-water (90:10; v/v) without any purification step...
September 21, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28929691/-deuterated-surrogate-and-uplc-ms-ms-method-for-simultaneous-determination-of-22-endocannabinoids-in-ovariectomized-rat-plasma
#4
Shi-Xie Xiang, Ying Xu, Jing-Jing Zhu, Zhi-Min Wang, Dong Zhang, Liang-Mian Chen, Wei-Hong Feng
A new method based on ultraperformance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) was developed for the simultaneous determination of 22 endocannabinoids(eCBs) and relevant compounds in ovariectomized rat plasma. After being extracted by solid-phase column(SPE), the plasma samples were detected by using UPLC-MS/MS. Analysis was carried out with ACQUITY UPLC BEH C₁₈ column. The mobile phase was 0.1% acetic acid solution(A)-acetonitrile and isopropanol(9∶1, B) for the gradient elution. In the positive ion multiple reaction monitoring(MRM) mode, deuterated reagents were taken as standard alternatives to calculate recoveries and simultaneously quantify 22 endocannabinoids...
November 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28929685/-simultaneous-determination-of-8-main-compounds-in-eclipta-herba-by-uplc
#5
Xue-Feng Hou, Shuai Sun, Gang Wang, Hui-Hui Qiu, Shu-Min Ding, Ying Liu, Dan Liu, Liang Feng, Xiao-Bin Jia
To establish an UPLC method for the simultaneous determination of 8 compounds in Eclipta Herba, such as isoquercitrin, luteoloside, demethylwedelolactone, isochlorogenic acid A, isochlorogenic acid C, luteolin, wedelolactone and apigenin. The experiment was performed with a Waters Acquity UPLC BEH C₁₈ (2.1 mm×100 mm, 1.7 μm) column by gradient elution of 0.1% formic acid in water and acetonitrile: 0-4 min,10%-13% A; 4-10 min, 13%-16% A; 10-13 min, 16%-25% A; 13-17 min, 25%-28% A; 17-20 min,28%-40% A;20-25 min,40%-95% A...
November 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28929658/-study-on-uplc-fingerprint-of-red-ginseng-based-on-good-separation-and-good-purity-of-chromatographic-peaks
#6
Wei-Hong Feng, Chun Li, Li-Na Ji, Li-Xin Yang, Li-Xin Rong, Liang-Mian Chen, Hong Yi, Zhi-Min Wang
This study is to establish the UPLC fingerprint of red ginseng. The separation was performed on a Waters Acquity BEH C₁₈ column (2.1 mm × 50 mm,1.7 μm), with the mobile phase consisting of acetonitrile and water for gradient elution. The detection wavelength was set at 203 nm. The UPLC fingerprint of red ginseng was established by using sample chromatography of 22 different purchase areas and 26 common peaks were found. Compared with the reference substances, 11 of the common peaks were identified as ginsenosides Rg₁, ginsenoside Re, ginsenoside Rf, ginsenoside Rh₁, ginsenoside Rg₂, ginsenoside Rb₁, 20(S)-ginsenoside F₁, ginsenoside Rb₂, ginsenoside Rb3, 20(S)-ginsenoside Rg₃ and 20(R)-ginsenoside Rg₃, respectively...
October 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28918324/simultaneous-determination-and-pharmacokinetic-study-of-twelve-bioactive-compounds-in-rat-plasma-after-intravenous-administration-of-xuebijing-injection-by-uhplc-q-orbitrap-hrms
#7
Lihua Zuo, Qiuyue Zhong, Zhenhui Wang, Zhi Sun, Lin Zhou, Zhuolun Li, Tanye Xu, Yingying Shi, Jinfa Tang, Shuzhang Du, Xiaojian Zhang
Xuebijing injection (XBJ) is a traditional Chinese herbal prescription widely used in the treatment of sepsis. Extensive studies revealed that the major bioactive constituents of XBJ injection, including phenolic acids, flavonoids, monoterpene glycosides, lactones and organic acids, play an important role in the treatment. In this study, a rapid, sensitive and accurate ultra high performance liquid chromatography - Q Exactive hybrid quadrupole - orbitrap high-resolution accurate mass spectrometry (UHPLC-Q-Orbitrap HRMS) method was developed for simultaneous determination of twelve bioactive compounds in rat plasma after intravenous administration of XBJ injection...
September 8, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28915937/ultra-high-performance-supercritical-fluid-chromatography-mass-spectrometry-procedure-for-analysis-of-monosaccharides-from-plant-gum-binders
#8
Volodymyr Pauk, Tomáš Pluháček, Vladimír Havlíček, Karel Lemr
The ultra-high performance supercritical fluid chromatography-mass spectrometry (UHPSFC/MS) procedure for analysis of native monosaccharides was developed. Chromatographic conditions were investigated to separate a mixture of four hexoses, three pentoses, two deoxyhexoses and two uronic acids. Increasing water content in methanol modifier to 5% and formic acid to 4% improved peak shapes of neutral monosaccharides and allowed complete elution of highly polar uronic acids in a single run. An Acquity HSS C18SB column outperformed other three tested stationary phases (BEH (silica), BEH 2-ethylpyridine, CSH Fluoro-Phenyl) in terms of separation of isomers and analysis time (4...
October 9, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28914671/endometrial-polyps-and-benign-endometrial-hyperplasia-present-increased-prevalence-of-dna-fragmentation-factors-40-and-45-dff40-and-dff45-together-with-the-antiapoptotic-b-cell-lymphoma-bcl-2-protein-compared-with-normal-human-endometria
#9
Tomasz Banas, Kazimierz Pitynski, Marcin Mikos, Joanna Cielecka-Kuszyk
DNA fragmentation factor 40 (DFF40) is a key executor of apoptosis. It localizes to the nucleus together with DNA fragmentation factor 45 (DFF45), which acts as a DFF40 inhibitor and chaperone. B-cell lymphoma (Bcl-2) protein is a proven antiapoptotic factor present in the cytoplasm. In this study, we aimed to investigate DFF40, DFF45, and Bcl-2 immunoexpression in endometrial polyps (EPs) and benign endometrial hyperplasia (BEH) tissue compared with that in normal proliferative endometrium (NPE) and normal secretory endometrium (NSE) as well as normal post menopausal endometrium (NAE)...
September 13, 2017: International Journal of Gynecological Pathology
https://www.readbyqxmd.com/read/28911669/simultaneous-determination-of-ascorbic-acid-and-caffeine-in-commercial-soft-drinks-using-reversed-phase-ultraperformance-liquid-chromatography
#10
Fatma Turak, Remziye Güzel, Erdal Dinç
A new reversed-phase ultraperformance liquid chromatography method with a photodiode array detector was developed for the quantification of ascorbic acid (AA) and caffeine (CAF) in 11 different commercial drinks consisting of one energy drink and 10 ice tea drinks. Separation of the analyzed AA and CAF with an internal standard, caffeic acid, was performed on a Waters BEH C18 column (100 mm × 2.1 mm, 1.7 μm i.d.), using a mobile phase consisting of acetonitrile and 0.2M H3PO4 (11:89, v/v) with a flow rate of 0...
April 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28906164/comparative-pharmacokinetics-study-of-anastrozole-after-single-administration-and-combination-with-celecoxib
#11
Xiaoyang Sun, Mingming Lv, Binjie Wang, Simin Zhao, Ping Guo, Shixiao Wang, Liqiang Gu, Xiaohui Chen
1. There are numerous investigations demonstrating that the cyclooxygenase-2 (COX-2) inhibitors might enhance the efficiency of anastrozole in breast cancer. Hence, this study was conducted to investigate the comparative pharmacokinetics of anastrozole after single administration and combination with celecoxib. 2. A simple protein precipitation procedure was adopted for the sample preparation with satisfactory extraction recovery for both anastrozole and the internal standard, and then anastrozole was separated and analysed on an ACQUITY BEH UPLC C18 column (50 × 2...
September 14, 2017: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
https://www.readbyqxmd.com/read/28906001/overcoming-interference-of-plasma-phospholipids-using-hybridspe-for-the-determination-of-trimetazidine-by-uplc-ms-ms
#12
Pravin G Vanol, Manish Yadav, Mallika Sanyal, Priyanka A Shah, Pranav S Shrivastav
An improved, precise and reliable ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method has been developed for the quantification of trimetazidine, using trimetazidine-d8 as the internal standard (IS). Interference due to plasma phospholipids during sample preparation was overcome by using hybrid solid phase extraction-phospholipid ultra cartridge. The mean extraction recovery of trimetazidine (98.66 %) and trimetazidine-d8 (97.63 %) from spiked plasma was consistent and reproducible...
September 14, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28883524/flat-building-blocks-for-flat-silicene
#13
Masae Takahashi
Silicene is the silicon equivalent of graphene, which is composed of a honeycomb carbon structure with one atom thickness and has attractive characteristics of a perfect two-dimensional π-conjugated sheet. However, unlike flat and highly stable graphene, silicene is relatively sticky and thus unstable due to its puckered or crinkled structure. Flatness is important for stability, and to obtain perfect π-conjugation, electron-donating atoms and molecules should not interact with the π electrons. The structural differences between silicene and graphene result from the differences in their building blocks, flat benzene and chair-form hexasilabenzene...
September 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28880257/inverse-association-between-cigarette-and-water-pipe-smoking-and-hypertension-in-an-elderly-population-in-iran-bushehr-elderly-health-programme
#14
M B Mehboudi, I Nabipour, K Vahdat, H Darabi, A Raeisi, N Mehrdad, R Heshmat, G Shafiee, B Larijani, A Ostovar
The collected data in Bushehr Elderly Health (BEH) Program which had detailed the data on participants' smoking status and habits, was analysed to investigate the association between smoking of both water pipes and cigarettes and hypertension in an elderly population. Three thousand elderly men and women who participated in the baseline assessment of the BEH Program-a prospective population-based study being conducted in Bushehr, Iran-were selected randomly through a multistage, stratified cluster sampling method...
September 7, 2017: Journal of Human Hypertension
https://www.readbyqxmd.com/read/28869284/ultra-performance-liquid-chromatography-tandem-mass-spectrometry-assay-for-determination-of-plasma-nomegestrol-acetate-and-estradiol-in-healthy-postmenopausal-women
#15
Sneha G Nair, Daxesh P Patel, Mallika Sanyal, Puran Singhal, Pranav S Shrivastav
A highly sensitive and selective ultra-performance liquid chromatography-tandem mass spectrometry method is described for the simultaneous determination of nomegestrol acetate (NOMAC), a highly selective progestogen, and estradiol (E2), a natural estrogen in human plasma. NOMAC was obtained from plasma by solid phase extraction, while E2 was first separated by liquid-liquid extraction with methyl tert-butyl ether followed by derivatization with dansyl chloride. Deuterated internal standards, NOMAC-d5 and E2-d4 were used for better control of extraction conditions and ionization efficiency...
September 4, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28855942/method-development-and-validation-of-hplc-tandem-mass-spectrometry-for-quantification-of-perindopril-arginine-and-amlodipine-besylate-combination-in-bulk-and-pharmaceutical-formulations
#16
Kalaiyarasi Duraisamy, K S Jaganathan, Marothu Vamsi Krishna
A well-characterized and fully validated ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometric (UHPLC-ESI-MS/MS) method was developed to reliably analyze combination of perindopril arginine and amlodipine besylate in bulk and tablet formulations. The chromatographic separation was achieved on a Waters ACQUITY UPLC(®) BEH C18 column with 1.7 μm particle packing which enabled the higher peak capacity, greater resolution, increased sensitivity, and higher speed of analysis using a volatile mobile phase ideally being at least 2 pH units below and above the perindopril arginine and amlodipine besylate pKa, respectively...
August 2017: Research in Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28847134/brominated-flame-retardants-in-black-plastic-kitchen-utensils-concentrations-and-human-exposure-implications
#17
Jiangmeng Kuang, Mohamed Abou-Elwafa Abdallah, Stuart Harrad
Concerns exist that restricted brominated flame retardants (BFRs) present in waste polymers may have, as a result of recycling, inadvertently contaminated items not required to meet flame retardancy regulations (e.g. plastic kitchen utensils). To investigate the extent to which kitchen utensils are contaminated with BFRs and the potential for resultant human exposure, we collected 96 plastic kitchen utensils and screened for Br content using a hand-held X-ray fluorescence (XRF) spectrometer. Only 3 out of 27 utensils purchased after 2011 contained detectable concentrations of Br (≥3μg/g)...
August 24, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28843937/determination-of-protein-unbound-active-rifampicin-in-serum-by-ultrafiltration-and-ultra-performance-liquid-chromatography-with-uv-detection-a-method-suitable-for-standard-and-high-doses-of-rifampicin
#18
Eleonora W J van Ewijk-Beneken Kolmer, Marga J A Teulen, Erik C A van den Hombergh, Nielka E van Erp, Lindsey H M Te Brake, Rob E Aarnoutse
Rifampicin is the most important antibiotic in use for the treatment of tuberculosis (TB). Preclinical and clinical data suggest that higher doses of rifampicin, resulting in disproportionally higher systemic exposures to the drug, are more effective. Serum concentrations of rifampicin are the intermediary link between the dose administered and eventual response and only protein-unbound (free) rifampicin is pharmacologically active. The objective of this work was to develop an ultra performance liquid chromatography assay for protein-unbound rifampicin in serum with ultrafiltration, carried out at a sample temperature of 37°C, suitable for measurement of concentrations achieved after currently used and higher doses of rifampicin...
August 4, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28843142/legacy-and-alternative-halogenated-flame-retardants-in-human-milk-in-europe-implications-for-children-s-health
#19
Eliška Čechová, Šimon Vojta, Petr Kukučka, Anton Kočan, Tomáš Trnovec, Ľubica Palkovičová Murínová, Marijke de Cock, Margot van de Bor, Joakim Askevold, Merete Eggesbø, Martin Scheringer
In this study, 10 polybrominated diphenyl ethers (PBDEs) and 19 alternative halogenated flame retardants (AFRs) were determined in >450 human milk samples across three European countries, representing northern, western and eastern Europe. This study provides first insights into the occurrence of selected AFRs in mother milk samples and compares them among three European countries. Sums of median concentrations of the most frequently detected PBDEs were 2.16, 0.88 and 0.45ngg(-1) lipid weight (lw) in Norway, the Netherlands and Slovakia, respectively...
August 23, 2017: Environment International
https://www.readbyqxmd.com/read/28840252/the-role-of-centromere-defects-in-cancer
#20
Thian Thian Beh, Paul Kalitsis
The accurate segregation of chromosomes to daughter cells is essential for healthy development to occur. Imbalances in chromosome number have long been associated with cancers amongst other medical disorders. Little is known whether abnormal chromosome numbers are an early contributor to the cancer progression pathway. Centromere DNA and protein defects are known to impact on the fidelity of chromosome segregation in cell and model systems. In this chapter we discuss recent developments in understanding the contribution of centromere abnormalities at the protein and DNA level and their role in cancer in human and mouse systems...
2017: Progress in Molecular and Subcellular Biology
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