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https://www.readbyqxmd.com/read/28205294/simultaneous-determination-of-the-bioactive-components-in-rat-plasma-by-uplc-ms-ms-and-application-in-pharmacokinetic-studies-after-oral-administration-of-radix-scutellariae-extract
#1
Jin-Hua Tao, Jun Xu, Shu Jiang, Yong Ling, Dong-Geng Wang
A highly sensitive and rapid ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) has been developed and validated for simultaneous quantification of the four main bioactive compounds, i.e., baicalin, baicalein, wogonoside and wogonin in rat plasma after oral administration of Radix scutellariae extract. Clarithromycin was used as an internal standard (IS). Plasma samples were processed by protein precipitation with methanol. The separation was performed on an Acquity BEH C18 column (100 mm × 2...
February 16, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28193468/using-the-fundamentals-of-adsorption-to-understand-peak-distortion-due-to-strong-solvent-effect-in-hydrophilic-interaction-chromatography
#2
Fabrice Gritti, Jyotsna Sehajpal, Jacob Fairchild
The peak distortion observed in hydrophilic interaction chromatography (HILIC) may be caused by the sample diluent to mobile phase mismatch. The United States Pharmacopeia (USP) method for organic impurities in cetirizine HCl tablets calls for such a mismatch, having a higher concentration of strong solvent in the sample diluent than in the mobile phase. A significant peak deformation is reported for cetirizine (a second-generation antihistamine) when it is purified on a Ethylene Bridged Hybrid (BEH) HILIC column (4...
February 6, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28192758/ultra-high-performance-liquid-chromatography-tandem-mass-spectrometry-for-simple-and-simultaneous-quantification-of-cannabinoids
#3
Rohitash Jamwal, Ariel R Topletz, Bharat Ramratnam, Fatemeh Akhlaghi
Cannabis is used widely in the United States, both recreationally and for medical purposes. Current methods for analysis of cannabinoids in human biological specimens rely on complex extraction process and lengthy analysis time. We established a rapid and simple assay for quantification of Δ(9)-tetrahydrocannabinol (THC), cannabidiol (CBD), 11-hydroxy Δ(9)-tetrahydrocannabinol (11-OH THC) and 11-nor-9-carboxy-Δ(9)-tetrahydrocannbinol (THCCOOH) in human plasma by U-HPLC-MS/MS usingΔ9-tetrahydrocannabinol-D3 (THC-D3) as the internal standard...
February 6, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28178366/the-simultaneous-uplc-ms-ms-determination-of-emerging-drug-combination-candesartan-and-chlorthalidone-in-human-plasma-and-its-application
#4
Bhargav Patel, Arvind G Jangid, B N Suhagia, Nirmal Desai
A novel, precise, sensitive and accurate ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method has been developed for the simultaneous determination of novel drug combination, candesartan (CAN) and chlorthalidone (CHL) in human plasma. Chromatographic separation was achieved on Waters Acquity UPLC BEH C18 (50 mm × 2.1 mm, 1.7 µm). Mobile phase consisting of 1 mM Ammonium acetate in water: acetonitrile (20:80 v/v) was used. The total chromatographic runtime was 1...
February 8, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28167419/comparative-pharmacokinetic-profiles-of-selected-irreversible-tyrosine-kinase-inhibitors-neratinib-and-pelitinib-with-apigenin-in-rat-plasma-by-uplc-ms-ms
#5
Hadir M Maher, Nourah Z Alzoman, Shereen M Shehata, Ashwag O Abahussain
Neratinib (NER) and pelitinib (PEL) are irreversible tyrosine kinase inhibitors (TKIs) that have been recently employed in cancer treatment. Apigenin (API), among other flavonoids, is known to have antioxidant, anti-proliferative, and carcinogenic effect. API can potentiate the antitumor effect of chemotherapeutic agents and/or alleviate the side effects of many anticancer agents. Since TKIs are mostly metabolized by CYP3A4 enzymes and that API could alter the enzymatic activity, potential drug interactions could be expected following their co-aministration...
January 31, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28146302/simultaneous-determination-of-eight-bioactive-components-of-qishen-yiqi-dripping-pills-in-rat-plasma-using-uflc-ms-ms-and-its-application-to-a-pharmacokinetic-study
#6
Yaping Shao, Wen Zhang, Ling Tong, Jingyi Huang, Dongxiang Li, Wei Nie, Yan Zhu, Yunfei Li, Tao Lu
In this study, a rapid and reliable ultra-fast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) method was developed and validated for the simultaneous determination of eight active ingredients, including astragaloside IV (AIV), ononin (ONO), tanshinol (TSL), protocatechualdehyde (PCA), protocatechuic acid (PA), salvianolic acid D (SAD), rosmarinic acid (RA), and ginsenoside Rg1 (GRg1 ), in rat plasma. The plasma samples were pretreated by protein precipitation with acetonitrile. Chromatographic separation was performed on a Waters Acquity UPLC® BEH C18 column (1...
February 1, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28138851/discovery-of-rare-haplotypes-by-typing-millions-of-single-molecules-with-bead-emulsion-haplotyping-beh
#7
Elisabeth Palzenberger, Ronja Reinhardt, Leila Muresan, Barbara Palaoro, Irene Tiemann-Boege
Characterizing polymorphisms on single molecules renders the phase of different alleles, and thus, haplotype information. Here, we describe a high-throughput method to genotype hundreds-of thousands single molecules in parallel using bead-emulsion haplotyping (BEH). Haplotyping via BEH is an emulsion-PCR-based method that was adapted to amplify multiple DNA fragments on paramagnetic, microscopic beads within a compartment formed by an aqueous-oil emulsion. This generates beads covered by thousands of clonal copies from several polymorphic regions of an initial DNA molecule that are then genotyped with fluorescently labeled probes...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28135360/retinal-architecture-and-melanopsin-mediated-pupillary-response-characteristics-a-putative-pathophysiologic-signature-for-the-retino-hypothalamic-tract-in-multiple-sclerosis
#8
Ethan Meltzer, Peter V Sguigna, Adnan Subei, Shin Beh, Eric Kildebeck, Darrel Conger, Amy Conger, Marlen Lucero, Benjamin S Frohman, Ashley N Frohman, Shiv Saidha, Steven Galetta, Peter A Calabresi, Robert Rennaker, Teresa C Frohman, Randy H Kardon, Laura J Balcer, Elliot M Frohman
Importance: A neurophysiologic signature of the melanopsin-mediated persistent constriction phase of the pupillary light reflex may represent a surrogate biomarker for the integrity of the retinohypothalamic tract, with potential utility for investigating alterations in homeostatic mechanisms associated with brain disorders and implications for identifying new treatments. Objective: To characterize abnormalities of retinal architecture in patients with multiple sclerosis (MS) and corresponding alterations in the melanopsin-mediated sustained pupillary constriction response...
January 30, 2017: JAMA Neurology
https://www.readbyqxmd.com/read/28131203/temporal-evolution-of-the-trident-and-piglet-signs-of-osmotic-demyelination-syndrome
#9
Shin C Beh
Central pontine myelinolysis (CPM) is a potentially-devastating complication of rapid osmolar shifts, classically attributed to overlyaggressive correction of chronic hyponatremia. Magnetic resonance imaging (MRI) allowed earlier diagnosis of CPM, but most importantly, it has revealed that the odds of good functional recovery are surprisingly high. A trident shaped pontine lesion is a typical finding in CPM (the trident sign). The "piglet sign" is a much less well-known radiologic finding in CPM. Due to the rarity of CPM, very little has been published on the evolution of these MRI findings...
February 15, 2017: Journal of the Neurological Sciences
https://www.readbyqxmd.com/read/28131025/an-ultra-performance-liquid-chromatography-tandem-mass-spectrometry-method-for-the-therapeutic-drug-monitoring-of-isavuconazole-and-seven-other-antifungal-compounds-in-plasma-samples
#10
Balthazar Toussaint, Fanny Lanternier, Christian Woloch, Denis Fournier, Manon Launay, Eliane Billaud, Eric Dannaoui, Olivier Lortholary, Vincent Jullien
A new analytical method was developed for the routine Therapeutic Drug Monitoring of 8 antifungals compounds in 50μL of plasma: isavuconazole (ISZ), voriconazole (VRZ), posaconazole (PSZ), fluconazole (FCZ), caspofungin (CSF), flucytosine (5FC), itraconazole (ITZ) and its metabolite OH-itraconazole (OH-ITZ). After adding 50μL of the internal standard, which consisted in a mixture of the deuterated isotopes of the quantified compounds, the sample treatment consisted in a simple protein precipitation with 400μL of acetonitrile...
January 23, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28123325/metabolite-profiles-of-ginsenosides-rk1-and-rg5-in-zebrafish-using-ultraperformance-liquid-chromatography-quadrupole-time-of-flight-ms
#11
Wenwen Shen, Yingjie Wei, Daoquan Tang, Xiaobin Jia, Bin Chen
BACKGROUND: In the present study, metabolite profiles of ginsenosides Rk1 and Rg5 from red ginseng or red notoginseng in zebrafish were qualitatively analyzed with ultraperformance liquid chromatography/quadrupole-time-of-flight MS, and the possible metabolic were pathways proposed. METHODS: After exposing to zebrafish for 24 h, we determined the metabolites of ginsenosides Rk1 and Rg5. The chromatography was accomplished on UPLC BEH C18 column using a binary gradient elution of 0...
January 2017: Journal of Ginseng Research
https://www.readbyqxmd.com/read/28111064/a-rapid-uplc-ms-ms-assay-for-eicosanoids-in-human-plasma-application-to-evaluate-niacin-responsivity
#12
Tricia M Miller, Samuel M Poloyac, Kacey B Anderson, Brooke L Waddell, Erik Messamore, Jeffrey K Yao
A rapid and sensitive method using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed to simultaneously quantify hydroxyeicosatetraenoic (HETE), dihydroxyeicosatrienoic (DiHETrE), epoxyeicosatrienoic acid (EET), and prostaglandin metabolites of arachidonic acid in human plasma. Sample preparation consisted of solid phase extraction with Oasis HLB (30mg) cartridges for all metabolites. Separation of HETEs, EETs, and DiHETrEs was achieved on an Acquity UPLC BEH C18, 1...
January 18, 2017: Prostaglandins, Leukotrienes, and Essential Fatty Acids
https://www.readbyqxmd.com/read/28108082/analysis-of-drugs-of-abuse-in-human-plasma-using-microextraction-by-packed-sorbents-and-ultra-high-performance-liquid-chromatography
#13
P Fernández, M González, M Regenjo, A M Ares, A M Fernández, R A Lorenzo, A M Carro
A miniaturized and simple method based on digitally programmed microextraction by packed sorbent (eVol(®)-MEPS) coupled to ultra-performance liquid chromatography (UPLC) has been developed for quantitative determination of three synthetic cathinones and seven conventional drugs of abuse and metabolites. The influence of several extraction parameters, such as washing and elution solvents were tested. In addition important variables affecting MEPS performance, namely sample volume, sorbent drying time, washing solvent volume, elution volume, number of extraction cycles, sorbent phase and pH, were evaluated using an asymmetrical screening design...
February 17, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28106344/fast-separation-of-flavonoids-by-supercritical-fluid-chromatography-using-a-column-packed-with-a-sub-2%C3%A2-%C3%AE-m-particle-stationary-phase
#14
Bo Wang, Xiao-Hua Liu, Wei Zhou, Yan Hong, Shi-Lan Feng
The purpose of this study was to compare the effects of different chromatographic columns for the separation of seven flavonoids. Four different stationary phases are available, including bridged ethyl hybrid, BEH and the same hybrid phase modified with 2-ethylpyridine, CSH fluorophenyl, and HSS C18 SB. The analytes included calycosin, genistein, medicarpin, calycosin-7-O-β-d-glucoside, formononetin, formononetin-7-O-β-d-glucoside, and liquiritigenin. The CSH fluorophenyl column was determined to be the most suitable and provided the fastest separation within 17 min using gradient elution with carbon dioxide as the mobile phase and methanol as the co-solvent...
January 20, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28103026/benchmark-quality-atomization-energies-for-beh-and-beh2
#15
Monica Vasiliu, Kirk A Peterson, David A Dixon
The total atomization energies for BeH and BeH2 have been calculated using the Feller-Peterson-Dixon approach to better than ±1 kcal/mol. The calculations are based on CCSD(T) all-electron calculations extrapolated to the complete limit, and CCSDT and CCSDTQ corrections are included. A scalar relativistic correction and a diagonal Born-Oppenheimer correction are included. Accurate zero-point energies are used. The total atomization energies at 0 K are 47.7 kcal/mol for BeH and 140.0 kcal/mol for BeH2 with error of at most ±0...
February 3, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28098395/first-uhplc-ms-ms-method-coupled-with-automated-on-line-spe-for-quantification-both-of-tacrolimus-and-everolimus-in-peripheral-blood-mononuclear-cells-and-its-application-on-samples-from-co-treated-pediatric-patients
#16
Debora Pensi, Amedeo De Nicolò, Michele Pinon, Clarissa Pisciotta, Pier Luigi Calvo, Antonello Nonnato, Renato Romagnoli, Francesco Tandoi, Giovanni Di Perri, Antonio D'Avolio
Tacrolimus (TAC, FK-506) and everolimus (EVE, RAD001) are immunosuppressors used to treat pediatric patients undergoing liver transplantation. Their hematic TDM by liquid chromatography became standard practice. However, it does not always reflect concentrations at their active site. Our aim was to develop and validate a new method for the simultaneous TAC and EVE quantification into target cells: Peripheral-Blood-Mononuclear-Cells (PBMCs). PBMCs were collected using Cell-Preparation-Tubes; cells number and MCV were evaluated by an automatic cell counter...
January 18, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28095587/quantitative-determination-of-lactones-in-piper-methysticum-kava-kava-by-supercritical-fluid-chromatography
#17
Adele Murauer, Markus Ganzera
A fast and validated supercritical fluid chromatography method for the quantitative determination of major lactones in Piper methysticum, a plant used against nervous anxiety, stress, and restlessness, was developed. The baseline separation of dihydrokavain, demethoxyyangonin, kavain, yangonin, dihydromethysticin, and methysticin was possible in less than 4 min on an Aquity UPC(2) BEH 1.7 µm column, in combination with a mobile phase comprising CO2 and methanol with diethylamine. The column temperature had a great impact on the results because only at 70 °C could kavain and yangonin be fully resolved...
January 17, 2017: Planta Medica
https://www.readbyqxmd.com/read/28086842/socioeconomic-inequality-of-overweight-and-obesity-of-the-elderly-in-iran-bushehr-elderly-health-beh-program
#18
Alireza Raeisi, Mohammadbagher Mehboudi, Hossein Darabi, Iraj Nabipour, Bagher Larijani, Neda Mehrdad, Ramin Heshmat, Gita Shafiee, Farshad Sharifi, Afshin Ostovar
BACKGROUND: The objective of this population-based, large sample size study was to investigate the socioeconomic inequality of overweight and obesity among the elderly in Iran. METHODS: Baseline data of 3000 persons aged ≥60 years who participated in the Bushehr Elderly Health (BEH) program was analyzed. Overweight and obesity were defined as a body mass index (BMI) equal to or higher than 25 and 30, respectively. Socioeconomic status (SES) was measured by an asset index, constructed using principal component analysis, income, education level, and employment status...
January 13, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28064067/simultaneous-determination-of-imperatorin-and-its-metabolite-xanthotoxol-in-rat-plasma-and-urine-by-lc-ms-ms-and-its-application-to-pharmacokinetic-studies
#19
Lien Ngo, Phuong Tran, Seong-Ho Ham, Jung-Hee Cho, Hea-Young Cho, Yong-Bok Lee
An accurate, precise, selective, and sensitive liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous determination of imperatorin (IMP) and its metabolite, xanthotoxol (XAN), in rat plasma and urine samples. The analytes, along with psoralen as an internal standard, were determined by multiple reaction monitoring (MRM) operated in the positive electrospray ionization (ESI) mode. Chromatographic separation was performed on an Acquity UPLC BEH C18 column (50mm×2...
December 28, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28063187/characterization-of-degradation-products-of-silodosin-under-stress-conditions-by-liquid-chromatography-fourier-transform-mass-spectrometry
#20
Sukanya Pandeti, Tadigoppula Narender, Sripadi Prabhakar, Thota Jagadeswar Reddy
RATIONALE: Silodosin (SDN) is a novel α1 -adrenoceptor antagonist in the treatment of benign prostatic hyperplasia (BPH). The presence of degradation products in a drug affects not only the quality, but also the safety and efficacy of drug formulation. Thus, it is essential to develop an efficient analytical method which could be useful to selectively separate, identify and characterise of all possible degradation products of SDN which is mandatory in drug development processes. METHODS: SDN was subjected to forced degradation under hydrolytic (acid, base and neutral), oxidative, photolytic and thermal stress conditions...
March 30, 2017: Rapid Communications in Mass Spectrometry: RCM
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