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in vitro diagnostics

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https://www.readbyqxmd.com/read/29239532/a-3d-real-scale-biomimetic-and-biohybrid-model-of-the-blood-brain-barrier-fabricated-through-two-photon-lithography
#1
Attilio Marino, Omar Tricinci, Matteo Battaglini, Carlo Filippeschi, Virgilio Mattoli, Edoardo Sinibaldi, Gianni Ciofani
The investigation of the crossing of exogenous substances through the blood-brain barrier (BBB) is object of intensive research in biomedicine, and one of the main obstacles for reliable in vitro evaluations is represented by the difficulties at the base of developing realistic models of the barrier, which could resemble as most accurately as possible the in vivo environment. Here, for the first time, a 1:1 scale, biomimetic, and biohybrid BBB model is proposed. Microtubes inspired to the brain capillaries were fabricated through two-photon lithography and used as scaffolds for the co-culturing of endothelial-like bEnd...
December 14, 2017: Small
https://www.readbyqxmd.com/read/29239298/optimized-conditions-for-the-in-vitro-excystment-of-calicophoron-daubneyi-metacercariae
#2
Kathryn M Huson, Charlotte Wild, Caroline Fenn, Mark W Robinson
Paramphistomosis, caused by Calicophoron daubneyi, is an emerging infection of ruminants throughout Western Europe. Despite its prevalence, many questions remain regarding the basic biology of this parasite and how it interacts with its host. Consequently, there is a need to develop methods to study C. daubneyi in vitro to improve our understanding of rumen fluke biology. Towards this, we aimed to identify a suitable protocol for in vitro excystment of C. daubneyi metacercariae. Six methods that have been used to excyst metacercariae from a number of trematode species were tested with C...
December 14, 2017: Parasitology
https://www.readbyqxmd.com/read/29238371/application-of-the-amlprofiler-diagnostic-microarray-in-the-south-african-setting
#3
S S Kappala, M Alessandrini, T Matlhako, E Beltchev, R Pool, M S Pepper
Acute myeloid leukemia (AML) is characterized by proliferation of the myeloid lineage and accumulation of immature hematopoietic cells in the bone marrow and is typified by marked heterogeneity both in response to treatment and survival. AMLprofiler is a qualitative in vitro diagnostic microarray incorporating seven molecular biomarkers used to diagnose and predict posttherapy survival rates. In this study, we compared AMLprofiler to routine AML diagnostic methodologies employed in South Africa, focusing on consistency of the results, cost, and time to result...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29231845/maillard-proteomics-opening-new-pages
#4
REVIEW
Alena Soboleva, Rico Schmidt, Maria Vikhnina, Tatiana Grishina, Andrej Frolov
Protein glycation is a ubiquitous non-enzymatic post-translational modification, formed by reaction of protein amino and guanidino groups with carbonyl compounds, presumably reducing sugars and α-dicarbonyls. Resulting advanced glycation end products (AGEs) represent a highly heterogeneous group of compounds, deleterious in mammals due to their pro-inflammatory effect, and impact in pathogenesis of diabetes mellitus, Alzheimer's disease and ageing. The body of information on the mechanisms and pathways of AGE formation, acquired during the last decades, clearly indicates a certain site-specificity of glycation...
December 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29229275/frequency-of-treponema-pallidum-invasion-into-cerebrospinal-fluid-in-primary-or-secondary-early-stage-syphilis
#5
Mao Hagihara, Yuka Yamagishi, Hideo Kato, Yuichi Shibata, Arufumi Shiota, Daisuke Sakanashi, Hiroyuki Suematsu, Hiroki Watanabe, Nobuhiro Asai, Yusuke Koizumi, Tatsuro Furui, Seiichiro Takahashi, Koji Izumi, Hiroshige Mikamo
Frequency of Treponema pallidum invasion into cerebrospinal fluid (CSF) has not been clear at this present. Since it is impossible to culture T. pallidum in vitro at this present, we need molecular based-approach to detect it in CSF. Additionally, neurosyphilis is usually a late sequela, however it might result in asymptomatic neurosyphilis even at primary or secondary syphilis. This study was to reveal the frequency of T. pallidum invasion into CSF especially at primary or secondary syphilis with polymerase chain reaction (PCR) test...
December 8, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/29228867/long-non-coding-rna-ccat1-promotes-multiple-myeloma-progression-by-acting-as-a-molecular-sponge-of-mir-181a-5p-to-modulate-hoxa1-expression
#6
Li Chen, Ning Hu, Chao Wang, Hongmian Zhao, Yueli Gu
Multiple myeloma (MM) is the second most common hematological cancer all over the world. Long non-coding RNA (lncRNA) colon cancer associated transcript-1 (CCAT1) has been reported to play important roles in the development and progression of multiple human malignancies. However, little is known about its functional role and molecular mechanism in MM. The aim of this study was to investigate the clinical and biological significance of CCAT1 in MM. Our data showed that the relative expression levels of CCAT1 were significantly upregulated in MM tissues and cell lines compared with healthy donors and normal plasma cells (nPCs)...
December 11, 2017: Cell Cycle
https://www.readbyqxmd.com/read/29228655/lncrna-svugp2-suppresses-progression-of-hepatocellular-carcinoma
#7
Jiangfeng Hu, Chenlin Song, Bensong Duan, Xiaoyan Zhang, Dongliang Li, Liang Zhu, Hengjun Gao
Numerous studies indicate that long noncoding RNAs (lncRNAs) are dysregulated in hepatocellular carcinoma (HCC) and might serve as potential diagnostic biomarkers and therapeutic targets of HCC. Therefore, it is interesting to globally identify the lncRNAs altered in HCC. In our study, we used microarray to profile the levels of lncRNAs and mRNAs in three pairs of HCC and their adjacent noncancerous samples. We found lncRNA-SVUGP2, which is a splice variant of the UGP2 gene, was down-regulated in HCC samples and correlates with a better prognosis in patients with HCC...
November 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29227160/inter-and-intra-examiner-reliability-of-bitewing-radiography-and-near-infrared-light-transillumination-for-proximal-caries-detection-and-assessment
#8
Friederike Litzenburger, Katrin Heck, Vinay Pitchika, Klaus W Neuhaus, Fabian N Jost, Reinhard Hickel, Anahita Jablonski-Momeni, Alexander Welk, Alexander Lederer, Jan Kühnisch
OBJECTIVES: The purpose of this in vitro study was to evaluate the inter- and intra-examiner reliability of digital bitewing (DBW) radiography and near-infrared light transillumination (NIRT) for proximal caries detection and assessment in posterior teeth. METHODS: From a pool of 85 patients, 100 corresponding pairs of DBW and NIRT images (~1/3 healthy, ~1/3 with enamel caries and ~1/3 with dentin caries) were chosen. Twelve dentists with different professional statuses and clinical experience repeated the evaluation in two blinded cycles...
December 11, 2017: Dento Maxillo Facial Radiology
https://www.readbyqxmd.com/read/29226682/first-evaluation-of-radioiodinated-flavonoids-as-necrosis-avid-agents-and-application-in-the-early-assessment-of-tumor-necrosis
#9
Jiajia Liang, Ziping Sun, Dongjian Zhang, Qiaomei Jin, Lingqiao Cai, Lin Ma, Wei Liu, Yicheng Ni, Jian Zhang, Zhiqi Yin
A rapid and accurate identification of necrotic tissues is of great importance to define disease severity, predict prognosis and monitor responses to therapies. To seek necrosis-avid agents with clinically translational potential, we first evaluated the necrosis avidity of flavonoids in rodent models of muscular, myocardial and tumoral necrosis. In this study, the necrosis avidity of eight radioiodinated 5,7-dihydroxyflavones was tested by ex vivo gamma counting, histochemical staining, and autoradiography in mouse models of ethanol-induced muscular necrosis...
December 11, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/29226594/selective-surface-enhanced-raman-scattering-for-quantitative-detection-of-lung-cancer-biomarkers-in-superparticle-mof-structure
#10
Xuezhi Qiao, Bensheng Su, Cong Liu, Qian Song, Dan Luo, Guang Mo, Tie Wang
Surface enhanced Raman scattering (SERS) is a trace detection technique that extends even to single molecule detection. Its potential application to the noninvasive recognition of lung malignancies by detecting volatile organic compounds (VOCs) that serve as biomarkers would be a breakthrough in early cancer diagnostics. This application, however, is currently limited by two main factors: (1) most VOC biomarkers exhibit only weak Raman scattering; and (2) the high mobility of gaseous molecules results in a low adsorptivity on solid substrates...
December 11, 2017: Advanced Materials
https://www.readbyqxmd.com/read/29226323/cops5-and-lasp1-synergistically-interact-to-down-regulate-14-3-3%C3%AF-expression-and-promote-colorectal-cancer-progression-via-activating-pi3k-akt-pathway
#11
Rui Zhou, Ziyun Shao, Jian Liu, Wanqi Zhan, Qingzu Gao, Zhihua Pan, Ling Wu, Lijun Xu, Yanqing Ding, Liang Zhao
Overexpression of LIM and SH3 protein 1 (LASP1) is required for colorectal cancer (CRC) development and progression. Here, C-Jun activation domain-binding protein-1 (Jab1), also known as COPS5, was verified as a new LASP1-interacting protein through yeast two-hybrid assay. The role of COPS5 in LASP1-mediated CRC progression remains unknown. GST pull-down assay indicated that the SH3 domain of LASP1 could directly bind to MPN domain of COPS5. In vitro gain- and loss-of-function analyses revealed the stimulatory role of COPS5 on CRC cell proliferation, migration and invasion...
December 11, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29225472/18f-fluorodeoxyglucose-uptake-predicts-met-expression-in-lung-adenocarcinoma
#12
Shuxian An, Xiang Zhou, Jianjun Liu, Gang Huang
Objective: MET is a member of the receptor tyrosine kinases. Several MET-targeting inhibitors and antagonistic antibodies have shown promising data in clinical trials of lung adenocarcinoma. Finding noninvasive diagnostic tools to estimate the status of MET is helpful in clinical practice. 18F-fluorodeoxyglucose positron emission tomography/computerized tomography (18F-FDG PET/CT) has been used routinely for the diagnosis and staging of tumors. However, the relationship between MET expression and 18F-FDG uptake has not been investigated yet...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/29225091/gene-expression-profiling-of-the-trim-protein-family-reveals-potential-biomarkers-for-indicating-tuberculosis-status
#13
Yanqing Chen, Shuhui Cao, Yong Sun, Chuanyou Li
Tripartite motif (TRIM) family proteins play important regulatory roles in innate immune responses, the dysregulation of which cause several infectious diseases. However, the role and function of TRIM family proteins during tuberculosis (TB) infection remains unclear. In this study, we employed real-time quantitative PCR to profile the transcript levels of 72 TRIM genes from a cohort of 5 active TB patients, 5 latent tuberculosis infection (LTBI) subjects, and 5 healthy controls (HCs) in an initial discovery phase...
December 7, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29224409/intellectual-property-considerations-for-molecular-diagnostic-development-with-emphasis-on-companion-diagnostics
#14
Harry Glorikian, Richard Jeremy Warburg, Kelly Moore, Jennifer Malinowski
The development of molecular diagnostics is a complex endeavor, with multiple regulatory pathways to consider and numerous approaches to development and commercialization. Companion diagnostics, devices which are "essential for the safe and effective use of a corresponding drug or diagnostic product" (see U.S. Food & Drug Administration, In Vitro Diagnostics - Companion Diagnostics, U.S. Dept. of Health & Human Services(2016), available at https://www.fda.gov/medicaldevices/productsandmedicalprocedures/invitrodiagnostics/ucm407297...
December 9, 2017: Expert Opinion on Therapeutic Patents
https://www.readbyqxmd.com/read/29221724/downregulation-of-klk13-promotes-the-invasiveness-and-metastasis-of-oesophageal-squamous-cell-carcinoma
#15
Qingfeng Lin, Weidong Mao, Qingquan Wu, Xiaoting He, Suqing Li, Yanxin Fan, Jie Chen, Tingting Feng, Xiufeng Cao
KLK13 downregulation occurs in tumour tissues in comparison with adjacent normal tissues from patients with oesophageal squamous cell carcinoma (OSCC). KLK13 mRNA levels were tested in OSCC tumour tissues and adjacent noncancerous tissues from 138 patients. In addition, the correlation between KLK13 mRNA levels and OSCC clinicopathologic features was analysed. KLK13 mRNA levels decreased notably in tumour tissues compared with those in adjacent noncancerous tissues. And decreased KLK13 mRNA levels indicated significant correlations with higher tumour grade, elevated TNM (UICC, 2009) stage classification, deeper infiltration and more lymph node metastases...
December 5, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29221133/endogenous-glutamine-decrease-is-associated-with-pancreatic-cancer-progression
#16
Cecilia Roux, Chiara Riganti, Sammy Ferri Borgogno, Roberta Curto, Claudia Curcio, Valeria Catanzaro, Giuseppe Digilio, Sergio Padovan, Maria Paola Puccinelli, Monica Isabello, Silvio Aime, Paola Cappello, Francesco Novelli
Pancreatic ductal adenocarcinoma (PDAC) is becoming the second leading cause of cancer-related death in the Western world. The mortality is very high, which emphasizes the need to identify biomarkers for early detection. As glutamine metabolism alteration is a feature of PDAC, its in vivo evaluation may provide a useful tool for biomarker identification. Our aim was to identify a handy method to evaluate blood glutamine consumption in mouse models of PDAC. We quantified the in vitro glutamine uptake by Mass Spectrometry (MS) in tumor cell supernatants and showed that it was higher in PDAC compared to non-PDAC tumor and pancreatic control human cells...
November 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29220150/functionalized-eu-iii-based-nanoscale-metal-organic-framework-to-achieve-near-ir-triggered-and-targeted-two-photon-absorption-photodynamic-therapy
#17
Jianguo Jia, Yang Zhang, Min Zheng, Changfu Shan, Huicheng Yan, Wenyu Wu, Xuan Gao, Bo Cheng, Weisheng Liu, Yu Tang
The postsynthetic-modified nanoscale metal-organic framework (NMOF) probes selected as potential drug delivery platforms and photodynamic therapy agents to fulfill the effective and safe treatment of neoplastic diseases have attracted increasing attention recently. Herein, a Eu(III)-based NMOF probe elaborately postsynthetically modified with a β-diketonate two-photon-absorbing (TPA) ligand is rationally designed and further functionalized by assembling the photosensitizer molecule (methylene blue, MB) in the pores and a cyclic peptide targeting motif on the surface of the NMOF, which could achieve highly efficient near-infrared (NIR)-triggered and -targeted photodynamic therapy (PDT)...
December 8, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/29214160/mir-217-is-a-useful-diagnostic-biomarker-and-regulates-human-podocyte-cells-apoptosis-via-targeting-tnfsf11-in-membranous-nephropathy
#18
Jing Li, Bin Liu, Hen Xue, Qiao Qiao Zhou, Ling Peng
Background: MicroRNAs have recently been verified as useful diagnostic biomarkers in various diseases. In this study, we investigated whether miR-217 is a useful diagnostic biomarker and the possible pathological mechanism of miR-217 in this disease. Methods: Patients with focal segmental glomerulosclerosis (FSGS), membranous nephropathy (MN), and diabetic nephropathy (DN) and control patients were enrolled in this study. The miR-217 inhibitor and mimics were transfected into human podocyte cells to investigate the pathological mechanism of miR-217 in this disease...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29213331/noninvasive-prenatal-diagnosis-of-fetal-aneuploidy-by-circulating-fetal-nucleated-red-blood-cells-and-extravillous-trophoblasts-using-silicon-based-nanostructured-microfluidics
#19
Chung-Er Huang, Gwo-Chin Ma, Hei-Jen Jou, Wen-Hsiang Lin, Dong-Jay Lee, Yi-Shing Lin, Norman A Ginsberg, Hsin-Fu Chen, Frank Mau-Chung Chang, Ming Chen
Background: Noninvasive prenatal testing (NIPT) based on cell-free DNA in maternal circulation has been accepted worldwide by the clinical community since 2011 but limitations, such as maternal malignancy and fetoplacental mosaicism, preclude its full replacement of invasive prenatal diagnosis. We present a novel silicon-based nanostructured microfluidics platform named as "Cell Reveal™" to demonstrate the feasibility of capturing circulating fetal nucleated red blood cells (fnRBC) and extravillous cytotrophoblasts (EVT) for cell-based noninvasive prenatal diagnosis (cbNIPD)...
2017: Molecular Cytogenetics
https://www.readbyqxmd.com/read/29212233/lncrna-xist-acts-as-a-tumor-suppressor-in-prostate-cancer-through-sponging-mir-23a-to-modulate-rkip-expression
#20
Yang Du, Xiao-Dong Weng, Lei Wang, Xiu-Heng Liu, Heng-Cheng Zhu, Jia Guo, Jin-Zhuo Ning, Cheng-Cheng Xiao
Accumulating evidences have indicated that aberrant expression of long non-coding RNAs (LncRNAs) is tightly associated with cancer development. Previous studies have reported that lncRNA XIST regulates tumor malignancies in several cancers. However, the underlying mechanism of XIST in prostate cancer remains unclear. In the current study, we found that XIST was down-regulated in prostate cancer specimens and cell lines. Low expression of XIST was correlated with poor prognosis and advanced tumor stage in prostate cancer patients...
November 7, 2017: Oncotarget
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