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https://www.readbyqxmd.com/read/27914320/biochemical-precursor-effects-on-the-fatty-acid-production-in-cell-suspension-cultures-of-theobroma-cacao-l
#1
O Parra, A M Gallego, A Urrea, L F Rojas, C Correa, L Atehortúa
Cocoa butter (CB) is composed of 96% palmitic, stearic, oleic, linoleic and linolenic fatty acids that are responsible for the hardness, texture and fusion properties of chocolate. Through in vitro plant cell culture it is possible to modify CB lipid profiles and to study the fatty acid biosynthesis pathway on a subcellular level, evaluating fundamental aspects to enhance in vitro fatty acid production in a specific and controlled way. In this research, culture media was supplemented with acetate, biotin, pyruvate, bicarbonate and glycerol at three different concentrations and the effects on the biomass production (g/L), cell viability, and fatty acids profile and production was evaluated in in vitro cell suspensions culture...
November 25, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27913021/climate-and-land-use-changes-effects-on-soil-organic-carbon-stocks-in-a-mediterranean-semi-natural-area
#2
Beatriz Lozano-García, Miriam Muñoz-Rojas, Luis Parras-Alcántara
A thorough knowledge of the effects of climate and land use changes on the soil carbon pool is critical to planning effective strategies for adaptation and mitigation in future scenarios of global climate and land use change. In this study, we used CarboSOIL model to predict changes in soil organic carbon stocks in a semi-natural area of Southern Spain in three different time horizons (2040, 2070, 2100), considering two general circulation models (BCM2 and ECHAM5) and three IPCC scenarios (A1b, A2, B2). The effects of potential land use changes from natural vegetation (Mediterranean evergreen oak woodland) to agricultural land (olive grove and cereal) on soil organic carbon stocks were also evaluated...
November 29, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27911894/head-lice-of-pygmies-reveal-the-presence-of-relapsing-fever-borreliae-in-the-republic-of-congo
#3
Nadia Amanzougaghene, Jean Akiana, Géor Mongo Ndombe, Bernard Davoust, Nardiouf Sjelin Nsana, Henri-Joseph Parra, Florence Fenollar, Didier Raoult, Oleg Mediannikov
BACKGROUND: Head lice, Pediculus humanus capitis, occur in four divergent mitochondrial clades (A, B, C and D), each having particular geographical distributions. Recent studies suggest that head lice, as is the case of body lice, can act as a vector for louse-borne diseases. Therefore, understanding the genetic diversity of lice worldwide is of critical importance to our understanding of the risk of louse-borne diseases. METHODOLOGY/PRINCIPAL FINDINGS: Here, we report the results of the first molecular screening of pygmies' head lice in the Republic of Congo for seven pathogens and an analysis of lice mitochondrial clades...
December 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27908796/detection-of-novel-gii-17-norovirus-in-argentina-2015
#4
Juan I Degiuseppe, Karina A Gomes, María F Hadad, Gabriel I Parra, Juan A Stupka
During the winter of 2014-2015 a novel GII.17 norovirus strain emerged as a cause of large gastroenteritis outbreaks in Asia; displacing the long-term predominant strain, GII.4. Although sporadically detected, the emerging GII.17 virus was described in North America and Europe. In this study, we describe the presence of this novel strain in Argentina (South America), and provide new information on the genetic diversity of GII.17 noroviruses. Ten stool samples from individuals (1-88years old; median: 5years old) experiencing gastroenteritis symptoms from San Martín de los Andes, Argentina were tested for Norovirus using RT-PCR...
November 28, 2016: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/27905634/-serological-evidence-of-leptospira-spp-dengue-hantavirus-and-arenavirus-infection-in-embera-kat%C3%A3-o-indigenous-population-colombia
#5
Berta Restrepo, Juan D Rodas, Carolina Montoya-Ruiz, Angélica M Zuluaga, Gabriel Parra-Henao, Piedad Agudelo-Flórez
We have developed a study in the Embera-Katío indigenous community and we found that the seropositivity for IgG antibodies to Leptospira spp. was 18.1%; to dengue was 61.1%; to arenavirus was 3.1% and to hantavirus was 1.5%. We conclude that this indigenous community live in conditions that promote the reemerging and emerging, infections.
August 2016: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
https://www.readbyqxmd.com/read/27903288/untangling-the-transmission-dynamics-of-primary-and-secondary-vectors-of-trypanosoma-cruzi-in-colombia-parasite-infection-feeding-sources-and-discrete-typing-units
#6
Carolina Hernández, Camilo Salazar, Helena Brochero, Aníbal Teherán, Luz Stella Buitrago, Mauricio Vera, Hugo Soto, Zulibeth Florez-Rivadeneira, Sussane Ardila, Gabriel Parra-Henao, Juan David Ramírez
BACKGROUND: Trypanosoma cruzi is the causative agent of Chagas disease. Due to its genetic diversity has been classified into six Discrete Typing Units (DTUs) in association with transmission cycles. In Colombia, natural T. cruzi infection has been detected in 15 triatomine species. There is scarce information regarding the infection rates, DTUs and feeding preferences of secondary vectors. Therefore, the aim of this study was to determine T. cruzi infection rates, parasite DTU, ecotopes, insect stages, geographical location and bug feeding preferences across six different triatomine species...
December 1, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27902433/escherichia-coli-clonal-group-a-cga-among-uropathogenic-infections-in-mexico-city
#7
Angel Manjarrez-Hernandez, José Molina-López, Sandra Gavilanes-Parra, Rigoberto Hernandez-Castro
Escherichia coli clonal group A (CGA) causes urinary tract and other extraintestinal infections in humans. CGA is an important cause of trimethoprim/sulfamethoxazole (SXT)-resistance in extraintestinal pathogens. We examined the extent to which resistance in this area is related to CGA dissemination of E. coli from urinary tract infections (UTIs) in Mexico City. The virulence backgrounds of the isolates were also characterized. In this study, the frequency of resistance to SXT used for UTI treatment was high (56-65%) and CGA isolates accounted for 9 of the 78 SXT-resistant isolates (11...
November 7, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27902386/establishment-of-memory-cd8-t-cells-with-live-attenuated-influenza-virus-laiv-across-different-vaccination-doses
#8
Zhongfang Wang, Lukasz Kedzierska, Simone Nuessing, Brendon Chua, Sergio M Quiñones-Parra, Victor C Huber, David Jackson, Paul G Thomas, Katherine Kedzierska
FluMist has been used in children and adults for more than ten years. As pre-existing CD8+ T-cell memory pools can provide heterologous immunity against distinct influenza viruses, it is important to understand influenza-specific CD8+ T-cell responses elicited by different LAIV regimens. In this study, we immunized mice intranasally (i.n.) with two different doses of live-attenuated PR8 virus (PR8 ts, H1N1), low and high, and then assessed protective efficacy by challenging animals with heterosubtypic X31-H3N2 virus at 6 weeks post-vaccination...
November 2, 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27898967/genomewide-association-analysis-of-growth-traits-in-charolais-beef-cattle
#9
F J Jahuey-Martínez, G M Parra-Bracamonte, A M Sifuentes-Rincón, J C Martínez-González, C Gondro, C A García-Pérez, L A López-Bustamante
The objective of this study was to perform a genomewide association study (GWAS) for growth traits in Charolais beef cattle and to identify SNP markers and genes associated with these traits. Our study included 855 animals genotyped using 76,883 SNP from the GeneSeek Genomic Profiler Bovine HD panel. The examined phenotypic data included birth, weaning, and yearling weights as well as pre- and postweaning ADG. After quality control, 68,337 SNP and 823 animals were retained in the analysis. The association analysis was performed using the principal components method via the egscore function of the GenABEL version 1...
November 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27896029/biology-of-tiny-animals-three-new-species-of-minute-salamanders-plethodontidae-thorius-from-oaxaca-mexico
#10
Gabriela Parra-Olea, Sean M Rovito, Mario García-París, Jessica A Maisano, David B Wake, James Hanken
We describe three new species of minute salamanders, genus Thorius, from the Sierra Madre del Sur of Oaxaca, Mexico. Until now only a single species, T. minutissimus, has been reported from this region, although molecular data have long shown extensive genetic differentiation among geographically disjunct populations. Adult Thorius pinicola sp. nov., T. longicaudus sp. nov., and T. tlaxiacus sp. nov. are larger than T. minutissimus and possess elliptical rather than oval nostrils; T. pinicola and T. longicaudus also have longer tails...
2016: PeerJ
https://www.readbyqxmd.com/read/27886391/estimate-of-the-total-costs-of-allergic-rhinitis-in-specialized-care-based-on-real-world-data-the-ferin-study
#11
C Colás, M Brosa, E Antón, J Montoro, A Navarro, M T Dordal, I Dávila, B Fernández-Parra, M D P Ibáñez, M Lluch-Bernal, V Matheu, C Rondón, M C Sánchez, A Valero
BACKGROUND: Despite the socioeconomic importance of allergic rhinitis (AR), very few prospective studies have been performed under conditions of routine clinical practice and with a sufficiently long observation period outside the clinical trial scenario. We prospectively estimated the direct and indirect costs of AR in patients attending specialized clinics in Spain. METHODS: Patients were recruited at random from allergy outpatient clinics in 101 health centers throughout Spain over 12 months...
November 25, 2016: Allergy
https://www.readbyqxmd.com/read/27885615/the-white-mullet-mugil-curema-as-biological-indicator-to-assess-environmental-stress-in-tropical-coastal-lagoons
#12
Alejandra García-Gasca, Julián Ríos-Sicairos, Rubí Hernández-Cornejo, Isabel Cunha, Jesús N Gutiérrez, Héctor Plascencia-González, Luz María García de la Parra, Selene Abad-Rosales, Miguel Betancourt-Lozano
Several coastal lagoons and estuaries in Mexico receive untreated domestic and industrial discharges which contain complex mixtures of contaminants. In order to assess the effects of chemical contamination, we used the White mullet (Mugil curema) as biological indicator. We worked in two estuaries located in Northwest Mexico: Urias (highly polluted) and Teacapan (less polluted, therefore used as reference site). We measured several endpoints at different levels of biological organization: vitellogenin transcription in males as biomarker of estrogenic contamination, as well as reproductive, morphological (deformities), morphometric, and meristic parameters...
December 0: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/27876763/growth-factor-and-co-receptor-release-by-structural-regulation-of-substrate-metalloprotease-accessibility
#13
Liseth M Parra, Monika Hartmann, Salome Schubach, Junzhi Ma, Peter Herrlich, Andreas Herrlich
Release of cytokines, growth factors and other life-essential molecules from precursors by a-disintegrin-and-metalloproteases (ADAMs) is regulated with high substrate-specificity. We hypothesized that this is achieved by cleavage-regulatory intracellular-domain (ICD)-modifications of the precursors. We show here that cleavage-stimuli-induced specific ICD-modifications cause structural substrate changes that enhance ectodomain sensitivity of neuregulin-1 (NRG1; epidermal-growth-factor) or CD44 (receptor-tyrosine-kinase (RTK) co-receptor) to chymotrypsin/trypsin or soluble ADAM...
November 23, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27875603/emr-based-intervention-improves-lead-screening-at-an-urban-family-medicine-practice
#14
Kathryn McGrath, Krys Foster, Patrick Doggett, Marc Altshuler, Jewel Osborne-Wu, Christine Castellan, Kelly Lopez, Pheobe Askie, Daniel Chung, Laura Parente, Yury Parra
BACKGROUND AND OBJECTIVES: Elevated blood lead levels have well-described detrimental effects to growth and development in children, yet screening rates remain low. We sought to determine if a reminder within the electronic health record (EHR) could change provider behavior and improve blood lead level (BLL) screening test ordering rates in an urban academic family medicine practice. METHODS: Baseline BLL test ordering rates were calculated for children ages 9-72 months...
November 2016: Family Medicine
https://www.readbyqxmd.com/read/27869392/-detection-of-aedes-albopictus-skuse-diptera-culicidae-in-the-municipality-of-istmina-choc%C3%A3-colombia
#15
José Joaquín Carvajal, Nildimar Alves Honorio, Silvia Patricia Díaz, Edinso Rafael Ruiz, Jimmy Asprilla, Susanne Ardila, Gabriel Parra-Henao
INTRODUCTION: Aedes albopictus is widely distributed around the world. Its introduction to the Americas occurred in 1985 and it is considered a potential vector of dengue viruses and one of the principal vectors of chikungunya virus. In Colombia, this species was reported for the first time in Leticia (Amazonas) in 1998, followed by Buenaventura (Valle del Cauca) in 2001, Barrancabermeja (Santander) in 2010, and Medellín (Antioquia) in 2011. So far, this species has been reported in ten departments of the country...
September 1, 2016: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/27869386/prediction-of-the-respiratory-syncitial-virus-epidemic-using-climate-variables-in-bogot%C3%A3-d-c
#16
Gilberto González-Parra, José F Querales, Diego Aranda
lntroduction: The respiratory syncitial virus is one of the most common causes of mortality in children and older adults in the world. Objective: To predict the initial week of outbreaks and to establish the most relevant climate variables using naive Bayes classifiers and receiver operating characteristic curves (ROC). Materials and methods: The initial dates of the outbreaks in children less than five years old for the period 2005-2010 were obtained for Bogotá, Colombia. We selected the climatological variables using a correlation matrix and we constructed 1,020 models using different climatological variables and data from different weeks previous to the initial outbreak...
September 1, 2016: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/27865608/benefit-in-long-term-response-and-mortality-of-treatment-with-intravenous-immunoglobulin-prior-to-plasmapheresis-in-peripheral-polyneuropathies
#17
I Parra-Salinas, V P González-Rodríguez, J A Gracia Pina, J J Gimeno Lozano, J A García-Erce
OBJECTIVES: The benefits of plasmapheresis (PA) for neurologic autoimmune diseases have been widely demonstrated. Little is known about the long-term neurologic prognosis and course after PA and immunosuppressive (IS) and/or intravenous immunoglobulin (IVIG) treatment. We aimed to analyse features associated with short-term response and long-term outcome and prognosis (neurologic status and mortality) of peripheral polyneuropathy (PP) and central nervous system acute inflammatory disease (CNSAID) treated with PA...
November 16, 2016: Transfusion Clinique et Biologique: Journal de la Société Française de Transfusion Sanguine
https://www.readbyqxmd.com/read/27859363/the-transcription-factor-obp4-controls-root-growth-and-promotes-callus-formation
#18
Elena Ramirez-Parra, Juan Perianez-Rodriguez, Sara Navarro-Neila, Inmaculada Gude, Miguel A Moreno-Risueno, Juan C Del Pozo
Plant growth and development require a continuous balance between cell division and differentiation. In root meristems, differentiated cells acquire specialized functions, losing their mitotic potential. Some plant cells, such as pericycle cells, have a remarkable plasticity to regenerate new organs. The molecular mechanisms underlying cell reprogramming are not completely known. In this work, a functional screening of transcription factors identified Arabidopsis OBP4 (OBF Binding Protein 4) as a novel regulator of root growth and cell elongation and differentiation...
November 17, 2016: New Phytologist
https://www.readbyqxmd.com/read/27858429/testing-hadronic-interactions-at-ultrahigh-energies-with-air-showers-measured-by-the-pierre-auger-observatory
#19
A Aab, P Abreu, M Aglietta, E J Ahn, I Al Samarai, I F M Albuquerque, I Allekotte, J D Allen, P Allison, A Almela, J Alvarez Castillo, J Alvarez-Muñiz, M Ambrosio, G A Anastasi, L Anchordoqui, B Andrada, S Andringa, C Aramo, F Arqueros, N Arsene, H Asorey, P Assis, J Aublin, G Avila, A M Badescu, C Baus, J J Beatty, K H Becker, J A Bellido, C Berat, M E Bertaina, X Bertou, P L Biermann, P Billoir, J Biteau, S G Blaess, A Blanco, J Blazek, C Bleve, H Blümer, M Boháčová, D Boncioli, C Bonifazi, N Borodai, A M Botti, J Brack, I Brancus, T Bretz, A Bridgeman, F L Briechle, P Buchholz, A Bueno, S Buitink, M Buscemi, K S Caballero-Mora, B Caccianiga, L Caccianiga, A Cancio, F Canfora, L Caramete, R Caruso, A Castellina, G Cataldi, L Cazon, R Cester, A G Chavez, A Chiavassa, J A Chinellato, J C Chirinos Diaz, J Chudoba, R W Clay, R Colalillo, A Coleman, L Collica, M R Coluccia, R Conceição, F Contreras, M J Cooper, S Coutu, C E Covault, J Cronin, R Dallier, S D'Amico, B Daniel, S Dasso, K Daumiller, B R Dawson, R M de Almeida, S J de Jong, G De Mauro, J R T de Mello Neto, I De Mitri, J de Oliveira, V de Souza, J Debatin, L Del Peral, O Deligny, N Dhital, C Di Giulio, A Di Matteo, M L Díaz Castro, F Diogo, C Dobrigkeit, J C D'Olivo, A Dorofeev, R C Dos Anjos, M T Dova, A Dundovic, J Ebr, R Engel, M Erdmann, M Erfani, C O Escobar, J Espadanal, A Etchegoyen, H Falcke, K Fang, G R Farrar, A C Fauth, N Fazzini, A P Ferguson, B Fick, J M Figueira, A Filevich, A Filipčič, O Fratu, M M Freire, T Fujii, A Fuster, F Gallo, B García, D Garcia-Pinto, F Gate, H Gemmeke, A Gherghel-Lascu, P L Ghia, U Giaccari, M Giammarchi, M Giller, D Głas, C Glaser, H Glass, G Golup, M Gómez Berisso, P F Gómez Vitale, N González, B Gookin, J Gordon, A Gorgi, P Gorham, P Gouffon, N Griffith, A F Grillo, T D Grubb, F Guarino, G P Guedes, M R Hampel, P Hansen, D Harari, T A Harrison, J L Harton, Q Hasankiadeh, A Haungs, T Hebbeker, D Heck, P Heimann, A E Herve, G C Hill, C Hojvat, N Hollon, E Holt, P Homola, J R Hörandel, P Horvath, M Hrabovský, T Huege, J Hulsman, A Insolia, P G Isar, I Jandt, S Jansen, C Jarne, J A Johnsen, M Josebachuili, A Kääpä, O Kambeitz, K H Kampert, P Kasper, I Katkov, B Keilhauer, E Kemp, R M Kieckhafer, H O Klages, M Kleifges, J Kleinfeller, R Krause, N Krohm, D Kuempel, G Kukec Mezek, N Kunka, A Kuotb Awad, D LaHurd, L Latronico, M Lauscher, P Lautridou, P Lebrun, R Legumina, M A Leigui de Oliveira, A Letessier-Selvon, I Lhenry-Yvon, K Link, L Lopes, R López, A López Casado, A Lucero, M Malacari, M Mallamaci, D Mandat, P Mantsch, A G Mariazzi, V Marin, I C Mariş, G Marsella, D Martello, H Martinez, O Martínez Bravo, J J Masías Meza, H J Mathes, S Mathys, J Matthews, J A J Matthews, G Matthiae, D Maurizio, E Mayotte, P O Mazur, C Medina, G Medina-Tanco, V B B Mello, D Melo, A Menshikov, S Messina, M I Micheletti, L Middendorf, I A Minaya, L Miramonti, B Mitrica, L Molina-Bueno, S Mollerach, F Montanet, C Morello, M Mostafá, C A Moura, G Müller, M A Muller, S Müller, I Naranjo, S Navas, P Necesal, L Nellen, A Nelles, J Neuser, P H Nguyen, M Niculescu-Oglinzanu, M Niechciol, L Niemietz, T Niggemann, D Nitz, D Nosek, V Novotny, H Nožka, L A Núñez, L Ochilo, F Oikonomou, A Olinto, D Pakk Selmi-Dei, M Palatka, J Pallotta, P Papenbreer, G Parente, A Parra, T Paul, M Pech, F Pedreira, J Pękala, R Pelayo, J Peña-Rodriguez, I M Pepe, L A S Pereira, L Perrone, E Petermann, C Peters, S Petrera, J Phuntsok, R Piegaia, T Pierog, P Pieroni, M Pimenta, V Pirronello, M Platino, M Plum, C Porowski, R R Prado, P Privitera, M Prouza, E J Quel, S Querchfeld, S Quinn, J Rautenberg, O Ravel, D Ravignani, B Revenu, J Ridky, M Risse, P Ristori, V Rizi, W Rodrigues de Carvalho, J Rodriguez Rojo, M D Rodríguez-Frías, D Rogozin, J Rosado, M Roth, E Roulet, A C Rovero, S J Saffi, A Saftoiu, H Salazar, A Saleh, F Salesa Greus, G Salina, J D Sanabria Gomez, F Sánchez, P Sanchez-Lucas, E M Santos, E Santos, F Sarazin, B Sarkar, R Sarmento, C Sarmiento-Cano, R Sato, C Scarso, M Schauer, V Scherini, H Schieler, D Schmidt, O Scholten, H Schoorlemmer, P Schovánek, F G Schröder, A Schulz, J Schulz, J Schumacher, S J Sciutto, A Segreto, M Settimo, A Shadkam, R C Shellard, G Sigl, O Sima, A Śmiałkowski, R Šmída, G R Snow, P Sommers, S Sonntag, J Sorokin, R Squartini, D Stanca, S Stanič, J Stapleton, J Stasielak, F Strafella, A Stutz, F Suarez, M Suarez Durán, T Sudholz, T Suomijärvi, A D Supanitsky, M S Sutherland, J Swain, Z Szadkowski, O A Taborda, A Tapia, A Tepe, V M Theodoro, C Timmermans, C J Todero Peixoto, L Tomankova, B Tomé, A Tonachini, G Torralba Elipe, D Torres Machado, P Travnicek, M Trini, R Ulrich, M Unger, M Urban, A Valbuena-Delgado, J F Valdés Galicia, I Valiño, L Valore, G van Aar, P van Bodegom, A M van den Berg, A van Vliet, E Varela, B Vargas Cárdenas, G Varner, J R Vázquez, R A Vázquez, D Veberič, V Verzi, J Vicha, M Videla, L Villaseñor, S Vorobiov, H Wahlberg, O Wainberg, D Walz, A A Watson, M Weber, A Weindl, L Wiencke, H Wilczyński, T Winchen, D Wittkowski, B Wundheiler, S Wykes, L Yang, T Yapici, D Yelos, E Zas, D Zavrtanik, M Zavrtanik, A Zepeda, B Zimmermann, M Ziolkowski, Z Zong, F Zuccarello
Ultrahigh energy cosmic ray air showers probe particle physics at energies beyond the reach of accelerators. Here we introduce a new method to test hadronic interaction models without relying on the absolute energy calibration, and apply it to events with primary energy 6-16 EeV (E_{CM}=110-170  TeV), whose longitudinal development and lateral distribution were simultaneously measured by the Pierre Auger Observatory. The average hadronic shower is 1.33±0.16 (1.61±0.21) times larger than predicted using the leading LHC-tuned models EPOS-LHC (QGSJetII-04), with a corresponding excess of muons...
November 4, 2016: Physical Review Letters
https://www.readbyqxmd.com/read/27855050/-adverse-events-during-diagnostic-and-therapeutic-pediatric-cardiac-catheterization
#20
Armando Zepeda-Arámbula, Lorenzo Gutiérrez-Cobian, Jorge Luis Villatoro-Fernández, Sandra Livier Pacheco-López, Alberto Tlacuilo-Parra
BACKGROUND: The aim of this paper is to determine the frequency of adverse events during heart catheterization, in both categories diagnostic and therapeutic procedures in children. METHODS: We review the clinical charts of those patients undergoing heart catheterization during the period from May 1 to October 31, 2014, in order to determine the frequency of adverse events presented within the first 24 hours after the procedure. RESULTS: During the six month period, 143 procedures were performed, of which 126 met the inclusion criteria...
2016: Revista Médica del Instituto Mexicano del Seguro Social
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