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https://www.readbyqxmd.com/read/29786748/protective-effects-of-dehydrocostuslactone-on-rat-hippocampal-slice-injury-induced-by-oxygen%C3%A2-glucose-deprivation-reoxygenation
#1
Qipeng Zhao, Ailing Chen, Xiaobo Wang, Zhuanzhuan Zhang, Yunsheng Zhao, Yu Huang, Shuanglai Ren, Yafei Zhu
The present study aimed to investigate the protective effects of dehydrocostuslactone (DHL) against rat hippocampal slice injury caused by oxygen‑glucose deprivation/reoxygenation (OGD/R). Rat hippocampal slice injury was induced by OGD/R in vitro, and the degree of injury was evaluated through a lactate dehydrogenase (LDH) assay and 2,3,5‑triphenyltetrazolium chloride (TTC) staining. The protein expression levels of B‑cell lymphoma-2 (Bcl‑2), Bcl‑2‑associated X protein (Bax), cytochrome c (cyt‑c), apoptotic protease activating factor 1 (apaf‑1), caspase‑9, caspase‑7, caspase‑3, sequestosome 1 (SQSTM1) and microtubule‑associated protein 1 light chain 3 (LC3) were analyzed through western blot analysis...
May 17, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29786746/luteolin-induces-myelodysplastic-syndrome%C3%A2-derived-cell-apoptosis-via-the-p53%C3%A2-dependent-mitochondrial-signaling-pathway-mediated-by-reactive-oxygen-species
#2
Weimin Dong, Yan Lin, Yang Cao, Yue Liu, Xiaobao Xie, Weiying Gu
Luteolin, a common dietary flavonoid, induces the apoptosis of cells in several types of cancer. However, its role in myelodysplastic syndrome (MDS) and the potential underlying mechanisms remain to be elucidated. To evaluate the potential benefit and underlying mechanisms of luteolin in MDS cells, the viability of SKM‑1 cells and primary bone marrow (PBM) mononuclear cells from patients with intermediate‑ or high‑risk MDS were assessed using a Cell Counting Kit‑8 assay. The apoptotic features of cell morphology were assessed using Wright‑Giemsa staining, DNA fragmentation was analyzed by agarose gel electrophoresis, and the extent of apoptosis was quantified by flow cytometry (FCM)...
May 18, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29786670/deubiquitinylase-usp47-promotes-rela-phosphorylation-and-survival-in-gastric-cancer-cells
#3
Lara Naghavi, Martin Schwalbe, Ahmed Ghanem, Michael Naumann
Every year, gastric cancer causes around 819,000 deaths worldwide. The incidence of gastric cancer in the western world is slowly declining, but the prognosis is unpromising. In Germany, the 5-year-survival rate is around 32%, and the average life span after diagnosis is 6 to 9 months. Therapy of gastric cancer patients comprises a gastrectomy and perioperative or adjuvant chemotherapy. However, resistance of gastric cancer cells to these agents is widespread; thus, improved chemotherapeutic approaches are required...
May 22, 2018: Biomedicines
https://www.readbyqxmd.com/read/29786653/role-for-cystathionine-%C3%AE-lyase-cse-in-an-ethanol-e-induced-lesion-in-fetal-brain-gsh-homeostasis
#4
Dhyanesh Patel, Marylatha Rathinam, Courtney Jarvis, Lenin Mahimainathan, George Henderson, Madhusudhanan Narasimhan
Earlier, we reported that gestational ethanol (E) can dysregulate neuron glutathione (GSH) homeostasis partially via impairing the EAAC1-mediated inward transport of Cysteine (Cys) and this can affect fetal brain development. In this study, we investigated if there is a role for the transulfuration pathway (TSP), a critical bio-synthetic point to supply Cys in E-induced dysregulation of GSH homeostasis. These studies utilized an in utero E binge model where the pregnant Sprague⁻Dawley (SD) rat dams received five doses of E at 3...
May 22, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29786113/overexpression-of-c%C3%A2-sis-inhibits-h2o2%C3%A2-induced-buffalo-rat-liver-cell-apoptosis-in-vitro-and-alleviates-liver-injury-in-a-rat-model-of-fulminant-hepatic-failure
#5
Hao Ding, Zhili Wen
The present study aimed to investigate the role of the C‑sis gene in the apoptosis of hepatocytes in vitro and in the liver function of a rat model of fulminant hepatic failure (FHF). Buffalo rat liver (BRL) cells were treated with hydrogen peroxide (H2O2) to induce apoptosis and then transfected with a C‑sis overexpression vector. A rat model of FHF was established, and C‑sis was overexpressed. The mRNA and protein expression of C‑sis were examined using reverse transcription‑polymerase chain reaction and western blot analyses, respectively...
May 17, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29785804/can-scsa-and-tunel-forecast-apoptosis-related-motility-depletion-in-asthenozoospermia
#6
Z Moradian Fard, M Naghdi, P Salehi, A Ajami, M R Deemeh, M H Meshkibaf
This study is an attempt to determine the power of SCSA and TUNEL for the evaluation of apoptosis status and apoptosis-related motility depletion in Asthenozoospermia. Fifty-one semen samples from Asthenozoospermic and 20 samples from fertile men participated in this study. SCSA and TUNEL were applied for the assessment of DNA integrity by flow cytometry. Annexin V conjugated with FITC labelling and FLICA method were used for the assessment of externalisation of phosphatidylserine and spermatozoon with active Caspase 3 respectively...
May 21, 2018: Andrologia
https://www.readbyqxmd.com/read/29785241/an-in-vivo-zebrafish-model-for-interrogating-ros-mediated-pancreatic-%C3%AE-cell-injury-response-and-prevention
#7
Abhishek A Kulkarni, Abass M Conteh, Cody A Sorrell, Anjali Mirmira, Sarah A Tersey, Raghavendra G Mirmira, Amelia K Linnemann, Ryan M Anderson
It is well known that a chronic state of elevated reactive oxygen species (ROS) in pancreatic β -cells impairs their ability to release insulin in response to elevated plasma glucose. Moreover, at its extreme, unmitigated ROS drives regulated cell death. This dysfunctional state of ROS buildup can result both from genetic predisposition and environmental factors such as obesity and overnutrition. Importantly, excessive ROS buildup may underlie metabolic pathologies such as type 2 diabetes mellitus. The ability to monitor ROS dynamics in β -cells in situ and to manipulate it via genetic, pharmacological, and environmental means would accelerate the development of novel therapeutics that could abate this pathology...
2018: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29785122/aloperine-executes-antitumor-effects-through-the-induction-of-apoptosis-and-cell-cycle-arrest-in-prostate-cancer-in-vitro-and-in-vivo
#8
Zhixin Ling, Han Guan, Zonghao You, Can Wang, Ling Hu, Lei Zhang, Yiduo Wang, Shuqiu Chen, Bin Xu, Ming Chen
Background: Prostate cancer (PCa) is one of the most common malignant diseases among male patients. Although androgen deprivation therapy remains the main treatment for PCa, most patients would inevitably progress to castration-resistant PCa, which is the main cause of cancer-related deaths. Thus, novel antitumor agents are urgently needed. Recent studies demonstrated that aloperine (ALO) as a natural alkaloid showed antitumor effects in other cancer types. However, the biological function and underlying mechanisms of ALO in PCa have not been investigated...
2018: OncoTargets and Therapy
https://www.readbyqxmd.com/read/29784872/sirt1-inhibits-rheumatoid-arthritis-fibroblast-like-synoviocyte-aggressiveness-and-inflammatory-response-via-suppressing-nf-%C3%AE%C2%BAb-pathway
#9
Guoqing Li, Zhongbing Xia, Ying Liu, Fanru Meng, Xia Wu, Yuxuan Fang, Chunwang Zhang, Dan Liu
Rheumatoid arthritis (RA) is an autoimmune disease of the joints characterized by synovial hyperplasia and chronic inflammation. Fibroblast-like synoviocytes (FLS) play a central role in RA initiation, progression, and perpetuation. Prior studies showed that sirtuin 1(SIRT1), a deacetylase participating in a broad range of transcriptional and metabolic regulations, may impact cell proliferation and inflammatory responses. However, the role of SIRT1 in RA-FLS was unclear. Here, we explored the effects of SIRT1 on the aggressiveness and inflammatory responses of cultured RA-FLS...
May 21, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/29784662/ibuprofen-decreases-spontaneous-activity-and-enhances-nerve-evoked-contractions-to-minimise-mitomycin-c-induced-bladder-dysfunction
#10
Eliza G West, Ryan Lang, Donna Sellers, Russ Chess-Williams, Catherine M McDermott
Inflammation may play a causal role in urological side effects reported following intravesical mitomycin C (MMC). Our aim was to investigate the effects of the non-steroidal anti-inflammatory drug ibuprofen (IBU) on the cytotoxic potency of MMC and the potential for IBU to protect against bladder dysfunction. Malignant (RT4, T24) and normal (UROtsa) urothelial lines were treated with MMC followed by ibuprofen, with cell viability and caspase-3 activity assessed. Female C57BL/6JArc mice (Saline/Control, MMC, Saline + IBU and MMC + IBU), received intravesical treatment (1hr) with saline or MMC (2mg/mL), with IBU (1mg/mL) delivered in drinking water (for 7-days)...
May 21, 2018: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/29784015/induction-of-multiple-myeloma-cancer-stem-cell-apoptosis-using-conjugated-anti-abcg2-antibody-with-epirubicin-loaded-microbubbles
#11
Fangfang Shi, Miao Li, Jing Wang, Di Wu, Meng Pan, Mei Guo, Jun Dou
BACKGROUND: Multiple myeloma (MM) currently remains largely incurable. Cancer stem cells (CSCs) are believed to be responsible for drug resistance and eventual relapse. In this study, we exploited a novel agent to evaluate its inhibitory effect on MM CSCs. METHODS: Epirubicin (EPI)-loaded lipid microbubbles (MBs) conjugated with anti-ABCG2 monoclonal antibody (EPI-MBs + mAb) were developed and their effect on MM 138- CD34- CSCs isolated from human MM RPMI 8226 cell line plus ultrasound exposure in vitro and in vivo in a nonobese diabetic/severe combined immunodeficient mouse model were assessed...
May 21, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29783958/sanguinarine-triggers-intrinsic-apoptosis-to-suppress-colorectal-cancer-growth-through-disassociation-between-strap-and-melk
#12
Xianling Gong, Zhihong Chen, Qinrui Han, Chunhui Chen, Linlin Jing, Yawei Liu, Liang Zhao, Xueqing Yao, Xuegang Sun
BACKGROUND: Previous studies showed sanguinarine induced apoptosis in CRC cells but did not define the underlying mechanisms. The purpose of this work was to determine the in vivo and in vitro effects of sanguinarine on CRC tumors and to elucidate the mechanism in regulating the intrinsic apoptosis. METHODS: Cell viability of CRC cell lines treated with sanguinarine was measured by MTT assay. Apoptotic cells stained with Annexin V and 7-AAD were detected by flow cytometry...
May 21, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29783729/chlorogenic-acid-improves-the-regorafenib-effects-in-human-hepatocellular-carcinoma-cells
#13
Maria Grazia Refolo, Catia Lippolis, Nicola Carella, Aldo Cavallini, Caterina Messa, Rosalba D'Alessandro
Chlorogenic acid (CGA) is a polyphenol present in many human dietary foods. Several studies indicated a beneficial role of CGA in the prevention of cancer and an enhancement of chemotherapy when combined with CGA in the treatment of human hepatocarcinoma (HCC). Drug toxicity, resistance and subsequent disease progression represent a problem in HCC management, although treatment with the multikinase inhibitor Regorafenib improved overall survival. This study focused on the evaluation of the effects of combined treatment using both low Regorafenib concentrations and CGA as natural compound in HCC cells...
May 19, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29782860/tetramethylpyrazine-attenuates-lipopolysaccharide-induced-cardiomyocyte-injury-via-improving-mitochondrial-function-mediated-by-14-3-3%C3%AE
#14
Bowei Huang, Jiegeng You, Yang Qiao, Zelong Wu, Dan Liu, Dong Yin, Huan He, Ming He
Lipopolysaccharide (LPS) is one of the many reasons that can cause myocardial injury. Our previous works have demonstrated that 14-3-3γ could protect myocardium against LPS-induced injury. Tetramethylpyrazine (TMP), an alkaloid found in Chinese herbs, exerts myocardial protection in many ways with multiple targets. We hypothesized that the cardioprotection of TMP against LPS-induced injury is attributed to upregulation of 14-3-3γ and improvement of mitochondrial function. To test the hypothesis, we investigated the effects of TMP on LPS-induced injury to cardiomyocytes by determining cell viability, LDH and caspase-3 activities, reactive oxygen species and MMP levels, mPTP openness, and apoptosis rate...
May 18, 2018: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29782637/luteolin-ameliorates-rat-myocardial-ischemia-reperfusion-injury-through-peroxiredoxin-ii-activation
#15
Bo Wei, Qiao Lin, Ya-Ge Ji, Yi-Can Zhao, Li-Na Ding, Wen-Juan Zhou, Li-Hua Zhang, Chuan-Yu Gao, Wen Zhao
BACKGROUND AND PURPOSE: Antioxidants provide a promising therapeutic effect for the cardiovascular disease. Luteolin (LUT), a polyphenolic bioflavonoid, is known to confer cardioprotection, although the underlying mechanism, especially the role of LUT on the antioxidant enzymes (e.g. peroxiredoxin family, PRXs), remains elusive. EXPERIMENTAL APPROACH: The current study was designed to evaluate the impact of LUT on MI/R injury in vivo and in vitro, as well as the underlying mechanism with a focus on PRXs signaling...
May 21, 2018: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29781715/andrographolide-exhibits-anticancer-potential-against-human-colon-cancer-cells-by-inducing-cell-cycle-arrest-and-programmed-cell-death-via-augmentation-of-intracellular-reactive-oxygen-species-level
#16
Imran Khan, Fahad Khan, Arshi Farooqui, Irfan A Ansari
Andrographolide, a diterpenoid lactone and a major constituent of Andrographis paniculata Nees, exhibits remarkable anticancer activity. However, the effect of andrographolide on colon cancer has not been completely elucidated yet. Thus, we investigated the chemopreventive potential of andrographolide in colon cancer HT-29 cells. The cytotoxic potential of andrographolide on HT-29 cells was determined by MTT assay, trypan blue exclusion assay, colony formation assay, and morphological analysis; and apoptotic property by DAPI and Hoechst staining, FITC-Annexin V assay, DNA fragmentation assay and caspase-3 activity assay...
May 21, 2018: Nutrition and Cancer
https://www.readbyqxmd.com/read/29781318/hypochlorite-modified-albumins-promote-cell-death-in-the-tubule-interstitium-in-rats-via-mitochondrial-damage-in-obstructive-nephropathy-and-the-protective-effects-of-antioxidant-peptides
#17
Zong-Rui Liu, Si-Qi Chen, Yao-Wei Zou, Xiao-Yu Wu, Hong-Ying Li, Xiao-Qiao Wang, Yue Shi, Hong-Xin Niu
A major feature of the injury sustained by the kidney during obstructive nephropathy is a profound induction of apoptosis in the tubular epithelium. In this study, we explored the central roles of mitochondria and the mechanism of the protective effect of the mitochondrial targeted peptides in tubular cell apoptosis and interstitial fibrosis during obstructive nephropathy. Unilateral ureter obstruction (UUO) was performed on rats, and the animals were randomly assigned to intravenous treatment with normal saline, rat serum albumin (RSA), or HOCl-rat serum albumin (HOCl-RSA) in the presence or absence of SS-31...
May 2018: Free Radical Research
https://www.readbyqxmd.com/read/29780828/effect-of-formaldehyde-on-human-middle-ear-epithelial-cells
#18
Shin Hye Kim, Ji-Woong Choi, Myung-Whan Suh, Jun Ho Lee, Seung-Ha Oh, Jae-Jun Song, Moo Kyun Park
Formaldehyde (FA) is a familiar indoor air pollutant found in everything from cosmetics to clothing, but its impact on the middle ear is unknown. This study investigated whether FA causes cytotoxicity, inflammation, or induction of apoptosis in human middle ear epithelial cells (HMEECs). Cell viability was investigated using the trypan blue assay and a cell counting kit (CCK-8) in HMEECs treated with FA for 4 or 24 h. The expression of genes encoding the inflammatory cytokine tumor necrosis factor alpha (TNF- α ) and mucin (MUC5AC) was analyzed using RT-PCR...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29780826/silibinin-induced-human-glioblastoma-cell-apoptosis-concomitant-with-autophagy-through-simultaneous-inhibition-of-mtor-and-yap
#19
Zhuan-Li Bai, Vincent Tay, Shu-Zhong Guo, Juan Ren, Mao-Guo Shu
Silibinin, also known as silybin, is the major flavonolignan isolated from Silybum marianum . Although previous reports demonstrated that silibinin exhibits significant tumor suppressor activities in various cancers by promoting cell apoptosis, it was also shown to trigger autophagy to counteract apoptosis induced by exogenous stresses in several types of cells. However, there is no report to address the role of silibinin induced autophagy in human A172 and SR glioblastoma cells. Our study showed that silibinin treatment not only inhibited the metabolic activities of glioblastoma cells but also promoted their apoptosis through the regulation of caspase 3 and PARP-1 in concentration- and time-dependent manners...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29780443/separase-inhibitor-sepin-1-inhibits-foxm1-expression-and-breast-cancer-cell-growth
#20
Nenggang Zhang, Debananda Pati
Sepin-1, a potent non-competitive inhibitor of separase, inhibits cancer cell growth, but the mechanisms of Sepin-1-mediated growth inhibition are not fully understood. Here we report that Sepin-1 hinders growth of breast cancer cells, cell migration, and wound healing. Inhibition of cell growth induced by Sepin-1 in vitro doesn't appear to be through apoptosis but rather due to growth inhibition. Following Sepin-1 treatment caspases 3 and 7 are not activated and Poly (ADP-ribose) polymerase (Parp) is not cleaved...
2018: Journal of Cancer Science & Therapy
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