keyword
MENU ▼
Read by QxMD icon Read
search

Campylobacter jejuni

keyword
https://www.readbyqxmd.com/read/28223978/function-and-regulation-of-the-c4-dicarboxylate-transporters-in-campylobacter-jejuni
#1
Marc M S M Wösten, Chris H A van de Lest, Linda van Dijk, Jos P M van Putten
C4-dicarboxylates are important molecules for the human pathogen C.jejuni, as they are used as carbon and electron acceptor molecules, as sugars cannot be utilized by this microaerophilic organism. Based on the genome analysis, C. jejuni may possess five different C4-dicarboxylate transporters: DctA, DcuA, DcuB, and two homologs of DcuC. Here, we investigated the regulation and function of various C4-dicarboxylate transporters in C. jejuni. Transcription of the dctA and dcuC homologs is constitutive, while dcuA and dcuB are both directly regulated by the two-component RacR/RacS system in response to limited oxygen availability and the presence of nitrate...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28222842/antimicrobial-use-for-and-resistance-of-zoonotic-bacteria-recovered-from-nonhuman-primates
#2
Jeffrey Kim, Dondrae J Coble, Gregory W Salyards, Julie K Bower, William J Rinaldi, Gail B Plauche, Gregory G Habing
As a growing threat to human and animal health, antimicrobial resistance (AMR) has become a central public-health topic. Largescale surveillance systems, such as the National Antimicrobial Resistance Monitoring System (NARMS), are now established to monitor and provide guidance regarding AMR, but comprehensive literature on AMR among NHP is sparse. This study provides data regarding current antimicrobial use strategies and the prevalence of AMR in zoonotic bacteria recovered from NHP within biomedical research institutions...
February 1, 2017: Comparative Medicine
https://www.readbyqxmd.com/read/28221919/variation-in-campylobacter-multilocus-sequence-typing-subtypes-from-chickens-as-detected-on-three-plating-media
#3
M E Berrang, S R Ladely, R J Meinersmann, J E Line, B B Oakley, N A Cox
The objective of this study was to compare subtypes of Campylobacter jejuni and Campylobacter coli detected on three selective Campylobacter plating media to determine whether each medium selected for different subtypes. Fifty ceca and 50 carcasses (representing 50 flocks) were collected from the evisceration line in a commercial broiler processing plant. Campylobacter was cultured and isolated from cecal contents and carcass rinses on Campy-Cefex, Campy Line, and RF Campylobacter jejuni/coli agars. When a positive result was obtained with all three media, one colony of the most prevalent morphology on each medium was selected for further analysis by full genome sequencing and multilocus sequence typing...
November 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28220790/in-vivo-genome-editing-with-a-small-cas9-orthologue-derived-from-campylobacter-jejuni
#4
Eunji Kim, Taeyoung Koo, Sung Wook Park, Daesik Kim, Kyoungmi Kim, Hee-Yeon Cho, Dong Woo Song, Kyu Jun Lee, Min Hee Jung, Seokjoong Kim, Jin Hyoung Kim, Jeong Hun Kim, Jin-Soo Kim
Several CRISPR-Cas9 orthologues have been used for genome editing. Here, we present the smallest Cas9 orthologue characterized to date, derived from Campylobacter jejuni (CjCas9), for efficient genome editing in vivo. After determining protospacer-adjacent motif (PAM) sequences and optimizing single-guide RNA (sgRNA) length, we package the CjCas9 gene, its sgRNA sequence, and a marker gene in an all-in-one adeno-associated virus (AAV) vector and produce the resulting virus at a high titer. CjCas9 is highly specific, cleaving only a limited number of sites in the human or mouse genome...
February 21, 2017: Nature Communications
https://www.readbyqxmd.com/read/28208612/enteric-pathogens-and-their-toxin-induced-disruption-of-the-intestinal-barrier-through-alteration-of-tight-junctions-in-chickens
#5
REVIEW
Wageha A Awad, Claudia Hess, Michael Hess
Maintaining a healthy gut environment is a prerequisite for sustainable animal production. The gut plays a key role in the digestion and absorption of nutrients and constitutes an initial organ exposed to external factors influencing bird's health. The intestinal epithelial barrier serves as the first line of defense between the host and the luminal environment. It consists of a continuous monolayer of intestinal epithelial cells connected by intercellular junctional complexes which shrink the space between adjacent cells...
February 10, 2017: Toxins
https://www.readbyqxmd.com/read/28202797/the-epiquant-framework-for-computing-the-epidemiological-concordance-of-microbial-subtyping-data
#6
Benjamin M Hetman, Steven K Mutschall, James E Thomas, Victor P J Gannon, Clifford G Clark, Frank Pollari, Eduardo N Taboada
A fundamental assumption in the use and interpretation of microbial subtyping results for public health investigations is that isolates that appear to be related based on molecular subtyping data are expected to share commonalities with respect to their origin, history and distribution. Critically, no approach currently exists for systematically assessing the underlying epidemiology of subtyping results. Our aim was to develop a method for directly quantifying the similarity between bacterial isolates using basic sampling metadata and to develop a framework for computing the epidemiologic concordance of microbial typing results...
February 15, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28196097/temporal-induction-of-pro-inflammatory-and-regulatory-cytokines-in-human-peripheral-blood-mononuclear-cells-by-campylobacter-jejuni-and-campylobacter-coli
#7
Eman Hamza, Sonja Kittl, Peter Kuhnert
Campylobacter jejuni along with C. coli are major cause of human gastroenteritis worldwide. So far, the human immune response against Campylobacter is not entirely clear. We hypothesize that it is coordinated by an interaction between pro-inflammatory and regulatory cytokines which is influenced by bacterial and host-individual differences. Accordingly, we used peripheral blood mononuclear cells (PBMC) from healthy donors to study the primary systemic immune response to C. jejuni and C. coli. PBMC were stimulated by different strains of C...
2017: PloS One
https://www.readbyqxmd.com/read/28194914/genetic-diversity-and-antimicrobial-resistance-profiles-of-campylobacter-coli-and-campylobacter-jejuni-isolated-from-broiler-chicken-in-farms-and-at-slaughter-in-central-italy
#8
S Pergola, M P Franciosini, F Comitini, M Ciani, S De Luca, S Bellucci, L Menchetti, P Casagrande Proietti
AIMS: Genetic diversity and antimicrobial resistance of C. coli and C. jejuni were investigated along the broiler chicken production chain in central Italy. METHODS AND RESULTS: Campylobacter spp. isolated from cloacal swabs in farms (n = 116) and from neck skin of chilled and eviscerated carcasses at slaughter (n = 24) were identified as C. coli (n = 99) and C. jejuni (n = 41) by multiplex PCR. Characterisation by s-AFLP revealed a specific genotype of Campylobacter for each farm...
February 14, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28194411/antibiotic-resistance-of-campylobacter-recovered-from-faeces-and-carcasses-of-healthy-livestock
#9
Akosua B Karikari, Kwasi Obiri-Danso, Enoch H Frimpong, Karen A Krogfelt
Campylobacter is of major significance in food safety and human and veterinary medicine. This study highlighted resistance situation in the area of veterinary public health in Ghana. Using selective mCCDA agar, isolates were confirmed phenotypically on API CAMPY and genotypically by multiplex PCR of IpxA gene. The susceptibility profile of species to common and relevant antibiotics was determined by the Kirby-Bauer disk diffusion method. Cattle, sheep, goat, and pig faecal samples analysed, respectively, yielded 13...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28188786/increased-glycosylation-efficiency-of-recombinant-proteins-in-escherichia-coli-by-auto-induction
#10
Ning Ding, Chunguang Yang, Shenxia Sun, Lichi Han, Yao Ruan, Longhua Guo, Xuejun Hu, Jianing Zhang
Escherichia coli cells have been considered as promising hosts for producing N-glycosylated proteins since the successful production of N-glycosylated protein in E. coli with the pgl (N-linked protein glycosylation) locus from Campylobacter jejuni. However, one hurdle in producing N-glycosylated proteins in large scale using E. coli is inefficient glycan glycosylation. In this study, we developed a strategy for the production of N-glycosylated proteins with high efficiency via an optimized auto-induction method...
February 8, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28182413/targeting-bacillosamine-biosynthesis-in-bacterial-pathogens-development-of-inhibitors-to-a-bacterial-amino-sugar-acetyltransferase-from-campylobacter-jejuni
#11
Joris W De Schutter, James P Morrison, Michael J Morrison, Alessio Ciulli, Barbara Imperiali
The glycoproteins of selected microbial pathogens often include highly modified carbohydrates such as 2,4-diacetamidobacillosamine (diNAcBac). These glycoconjugates are involved in host-cell interactions and may be associated with the virulence of medically significant Gram-negative bacteria. In light of genetic studies demonstrating the attenuated virulence of bacterial strains in which modified carbohydrate biosynthesis enzymes have been knocked out, we are developing small molecule inhibitors of selected enzymes as tools to evaluate whether such compounds modulate virulence...
February 22, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28178372/antimicrobial-activities-of-phenolic-extracts-derived-from-seed-coats-of-selected-soybean-varieties
#12
Rajaa Abutheraa, Navam Hettiarachchy, Geetha Kumar-Phillips, Ronny Horax, Pengyin Chen, Ruben Morawicki, Young Min Kwon
Soybean hulls or seed coats consist of complex carbohydrates, proteins, lipids, and polyphenols such as anthocyanidins, proanthocyanidins, and isoflavones. The polyphenolics in the seed coats give them various colors such as black, brown, green, yellow, or even a mottled appearance. In this study, the antimicrobial effects of phenolic extracts from the seed coats of different colored soybeans (yellow, dark brown, brown, and black) were evaluated against foodborne pathogens such as Salmonella Typhimurium, Escherichia coli O157:H7, and Campylobacter jejuni in broth-cultures as well as on chicken skin...
February 8, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28160162/isolation-identification-and-differentiation-of-campylobacter-spp-using-multiplex-pcr-assay-from-goats-in-khartoum-state-sudan
#13
Atif Elbrissi, Y A Sabeil, Khalda A Khalifa, Khalid Enan, Osama M Khair, A M El Hussein
The aim of this study was to identify and characterize thermophilic Campylobacter species in faecal samples from goats in Khartoum State, Sudan, by application of multiplex polymerase chain reaction. Campylobacteriosis is a zoonotic disease of global concern, and the organisms can be transmitted to human via food, water and through contact with farm animals and pets. There are five clinically related Campylobacter species: Campylobacter jejuni (C. jejuni). Campylobacter coli, Campylobacter lari, Campylobacter upsaliensis and Campylobacter fetus...
February 4, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28157786/campylobacter-jejuni-and-associated-immune-mechanisms-short-term-effects-and-long-term-implications-for-infants-in-low-income-countries
#14
Amanda E Schnee, William A Petri
PURPOSE OF REVIEW: Campylobacter jejuni is recognized as one of the most common causes of food-borne gastrointestinal illness worldwide, resulting in a self-limiting dysentery in developed countries. However, it is increasingly gaining attention due to its association with postinfectious complications such as Guillain-Barré Syndrome and recently recognized importance in early childhood diarrhea in developing countries. We hypothesize that the inflammation mediated by C. jejuni infection causes environmental enteric dysfunction, and with contribution from diet and the host, microbiome may be responsible for growth faltering in children and developmental disability...
February 2, 2017: Current Opinion in Infectious Diseases
https://www.readbyqxmd.com/read/28153902/draft-genome-sequence-of-campylobacter-jejuni-11168h
#15
Sarah E Macdonald, Ozan Gundogdu, Nick Dorrell, Brendan W Wren, Damer Blake, Richard Stabler
Campylobacter jejuni is the most prevalent cause of food-borne gastroenteritis in the developed world. The reference and original sequenced strain C. jejuni NCTC11168 has low levels of motility compared to clinical isolates. Here, we describe the draft genome of the laboratory derived hypermotile variant named 11168H.
February 2, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28144238/cj1199-affect-the-development-of-erythromycin-resistance-in-campylobacter-jejuni-through-regulation-of-leucine-biosynthesis
#16
Haihong Hao, Fei Li, Jing Han, Steven L Foley, Menghong Dai, Xu Wang, Yulian Wang, Lingli Huang, Yawei Sun, Zhenli Liu, Zonghui Yuan
The aim of this study was to reveal the biological function of Cj1199 which was overexpressed in the laboratory induced erythromycin resistant strains. The Cj1199 deletion mutant (ΦCj1199) was constructed via insertional inactivation from its parent strain Campylobacter jejuni NCTC11168. The ΦCj1199 and NCTC11168 were then subjected to microarray and real-time PCR to find gene pathway of Cj1199. The antimicrobial susceptibility, antimicrobial resistance development, growth characteristics and leucine metabolism were examined to confirm the biological function of Cj1199...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28139250/knowledge-gaps-in-host-parasite-interaction-preclude-accurate-assessment-of-meat-borne-exposure-to-toxoplasma-gondii
#17
M Crotta, G Limon, D P Blake, J Guitian
Toxoplasma gondii is recognized as a widely prevalent zoonotic parasite worldwide. Although several studies clearly identified meat products as an important source of T. gondii infections in humans, quantitative understanding of the risk posed to humans through the food chain is surprisingly scant. While probabilistic risk assessments for pathogens such as Campylobacter jejuni, Listeria monocytogenes or Escherichia coli have been well established, attempts to quantify the probability of human exposure to T...
December 20, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28135131/health-survey-of-free-ranging-raccons-procyon-lotor-in-central-park-new-york-new-york-usa-implications-for-human-and-domestic-animal-health
#18
Kimberly L Rainwater, Krysten Marchese, Sally Slavinski, Lee A Humberg, Edward J Dubovi, Jodie A Jarvis, Denise McAloose, Paul P Calle
We conducted health assessments on 113 free-ranging raccoons ( Procyon lotor ) in Central Park, New York City, in February 2010, September 2010, and November 2011 in conjunction with a trap-vaccinate-release program to control a raccoon rabies epizootic. Five individuals were sampled at two time points for 118 raccoon examinations in total. We tested 13 of 13 and 8 of 13 euthanized raccoons for rabies and canine distemper virus, respectively, by antigen testing on brain tissue; all were negative for both viruses...
January 30, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28131954/identification-and-initial-characterisation-of-a-protein-involved-in-campylobacter-jejuni-cell-shape
#19
Diane Esson, Srishti Gupta, David Bailey, Paul Wigley, Amy Wedley, Alison E Mather, Guillaume Méric, Pietro Mastroeni, Samuel K Sheppard, Nicholas R Thomson, Julian Parkhill, Duncan J Maskell, Graham Christie, Andrew J Grant
Campylobacter jejuni is the leading cause of bacterial food borne illness. While helical cell shape is considered important for C. jejuni pathogenesis, this bacterium is capable of adopting other morphologies. To better understand how helical-shaped C. jejuni maintain their shape and thus any associated colonisation, pathogenicity or other advantage, it is first important to identify the genes and proteins involved. So far, two peptidoglycan modifying enzymes Pgp1 and Pgp2 have been shown to be required for C...
January 25, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28128070/antimicrobial-use-for-and-resistance-of-zoonotic-bacteria-recovered-from-nonhuman-primates
#20
Jeffrey Kim Dondrae J Coble Gregory W Salyards Julie K Bower William J Rinaldi Gail B Plauche And Gregory G Habing
As a growing threat to human and animal health, antimicrobial resistance (AMR) has become a central public-health topic. Largescale surveillance systems, such as the National Antimicrobial Resistance Monitoring System (NARMS), are now established to monitor and provide guidance regarding AMR, but comprehensive literature on AMR among NHP is sparse. This study provides data regarding current antimicrobial use strategies and the prevalence of AMR in zoonotic bacteria recovered from NHP within biomedical research institutions...
January 26, 2017: Comparative Medicine
keyword
keyword
119522
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"