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Campylobacter jejuni

Marine Meunier, Muriel Guyard-Nicodème, Estelle Vigouroux, Typhaine Poezevara, Véronique Béven, Ségolène Quesne, Michel Amelot, Alberto Parra, Marianne Chemaly, Daniel Dory
Vaccination of broilers is one of the potential ways to decrease Campylobacter intestinal loads and therefore may reduce human disease incidence. Despite many studies, no efficient vaccine is available yet. Using the reverse vaccinology strategy, we recently identified new vaccine candidates whose immune and protective capacities need to be evaluated in vivo. Therefore, the goal of the present study was to develop and evaluate an avian subunit vaccine protocol for poultry against Campylobacter jejuni. For this, flagellin was used as vaccine antigen candidate...
March 16, 2018: Vaccine
Qian Zhang, Satoshi Ishii
Quantitative polymerase chain reaction (qPCR) is now commonly used to detect fecal indicator bacteria (FIB) as well as pathogens in water samples. However, DNA loss during sample processing can cause underestimation of target genes. In this study, we created a sample process control strain (SPC) by genetically engineering a non-pathogenic, Gram-negative bacterium Pseudogulbenkiania sp. strain NH8B. The SPC strain, named NH8B-1D2, has a kanamycin-resistance gene inserted to one of the 23S rRNA genes. To specifically quantify the SPC strain, a new TaqMan qPCR assay was developed...
March 10, 2018: Water Research
Nadia A Al-Banna, Farhan Cyprian, M John Albert
Campylobacter jejuni is an important enteric pathogen that causes diarrheas of different degrees of severity and several extra-intestinal manifestations, including Guillain-Barre syndrome. The variability of disease outcomes is thought to be linked to the immune response induced by C. jejuni. The virulence factors of C. jejuni induce a pro-inflammatory response, that is initiated by the intestinal epithelial cells, propagated by innate immune cells and modulated by the cells of the adaptive immune response...
March 10, 2018: Cytokine & Growth Factor Reviews
Kirill V Korneev, Anna N Kondakova, Ekaterina N Sviriaeva, Nikita A Mitkin, Angelo Palmigiano, Andrey A Kruglov, Georgy B Telegin, Marina S Drutskaya, Luisa Sturiale, Domenico Garozzo, Sergei A Nedospasov, Yuriy A Knirel, Dmitry V Kuprash
Toll-like receptor 4 (TLR4) initiates immune response against Gram-negative bacteria upon specific recognition of lipid A moiety of lipopolysaccharide (LPS), the major component of their cell wall. Some natural differences between LPS variants in their ability to interact with TLR4 may lead to either insufficient activation that may not prevent bacterial growth, or excessive activation which may lead to septic shock. In this study we evaluated the biological activity of LPS isolated from pathogenic strain of Campylobacter jejuni , the most widespread bacterial cause of foodborne diarrhea in humans...
2018: Frontiers in Cellular and Infection Microbiology
Samantha J Lawton, Allison M Weis, Barbara A Byrne, Heather Fritz, Conor C Taff, Andrea K Townsend, Bart C Weimer, Asli Mete, Sarah Wheeler, Walter M Boyce
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was compared to conventional biochemical testing methods and nucleic acid analyses (16S rDNA sequencing, hippurate hydrolysis gene testing, whole genome sequencing [WGS]) for species identification of Campylobacter isolates obtained from chickens ( Gallus gallus domesticus, n = 8), American crows ( Corvus brachyrhynchos, n = 17), a mallard duck ( Anas platyrhynchos, n = 1), and a western scrub-jay ( Aphelocoma californica, n = 1)...
March 1, 2018: Journal of Veterinary Diagnostic Investigation
Mirella Luciani, Maria Schirone, Ottavio Portanti, Pierina Visciano, Gisella Armillotta, Rosanna Tofalo, Giovanna Suzzi, Luigia Sonsini, Tiziana Di Febo
In this study, a new and alternative method based on monoclonal antibodies (MAbs) for the rapid detection of Yersinia enterocolitica O:8 was developed. This microorganism is an emerging foodborne pathogen causing gastrointestinal disease in humans. The transmission can occur through contaminated food such as raw or undercooked meat, milk and dairy products, water and fresh vegetables. Nine MAbs (46F7, 54B11, 54C11, 62D10, 64C7, 64C10, 72E8, 72E10, 72G6) were characterized and selected versus Y. enterocolitica O:8, and only 2 of them showed also a weak cross-reaction with Campylobacter jejuni...
August 2018: Food Microbiology
Chris A Whitehouse, Shenia Young, Cong Li, Chih-Hao Hsu, Gordon Martin, Shaohua Zhao
Whole genome sequencing (WGS) has become a rapid and affordable tool for public health surveillance and outbreak detection. In this study, we used the Illuminia MiSeq® to sequence 589 Campylobacter isolates obtained in 2015 from retail poultry meats as part of the National Antimicrobial Resistance Monitoring System (NARMS). WGS data were used to identify the Campylobacter species and to compare the concordance between resistance genotypes and phenotypes. WGS accurately identified 386 C. jejuni and 203 C. coli using gyrA sequence information...
August 2018: Food Microbiology
M J Sylte, M H Inbody, T A Johnson, T Looft, J E Line
Consumption of contaminated poultry products is the main source of human campylobacteriosis, for which Campylobacter jejuni is responsible for 90% of human cases. Although chickens are believed to be a main source of human exposure to C. jejuni, turkeys also contribute to cases of human infection. Little is known about the kinetics of C. jejuni intestinal colonization in turkeys, or best selective media for their recovery. Enumeration of C. jejuni from intestinal samples can be challenging because most selective Campylobacter media support the growth of non-Campylobacter organisms...
March 5, 2018: Poultry Science
N R Efimochkina, V V Stetsenko, I V Bykova, Yu M Markova, A S Polyanina, A I Aleshkina, S A Sheveleva
Specific features for the development of resistance in Campylobacter jejuni strains were studied after treatment with antibiotics of 6 pharmacological groups. Populations of 18 native strains of C. jejuni (isolated from raw poultry products) and their subcultures (obtained after 2-3-fold stress exposures to antimicrobial agents in subinhibitory doses) were examined to evaluate the expression of phenotypic antibiotic resistance. Genotypic properties of strains were studied by the PCR with primers that detect the presence of genes for resistance to aminoglycosides (aphA-1, aphA-3, and aphA-7), tetracyclines (tetO), and quinolones (GZgyrA)...
March 5, 2018: Bulletin of Experimental Biology and Medicine
Gaurav Dugar, Ryan T Leenay, Sara K Eisenbart, Thorsten Bischler, Belinda U Aul, Chase L Beisel, Cynthia M Sharma
Cas9 nucleases naturally utilize CRISPR RNAs (crRNAs) to silence foreign double-stranded DNA. While recent work has shown that some Cas9 nucleases can also target RNA, RNA recognition has required nuclease modifications or accessory factors. Here, we show that the Campylobacter jejuni Cas9 (CjCas9) can bind and cleave complementary endogenous mRNAs in a crRNA-dependent manner. Approximately 100 transcripts co-immunoprecipitated with CjCas9 and generally can be subdivided through their base-pairing potential to the four crRNAs...
March 1, 2018: Molecular Cell
Jurgita Aksomaitiene, Sigita Ramonaite, John E Olsen, Mindaugas Malakauskas
Recently, the number of reports on isolation of ciprofloxacin resistant Campylobacter jejuni has increased worldwide. The aim of this study was to determine the prevalence of resistance to ciprofloxacin and its genetic determinants among C. jejuni isolated from humans ( n = 100), poultry products ( n = 96) and wild birds ( n = 96) in Lithuania. 91.4% of the C. jejuni isolates were phenotypically resistant to ciprofloxacin. DNA sequence analyses of the gyrA gene from 292 isolates revealed that a change in amino acid sequence, Thr86Ile, was the main substition conferring resistance to ciprofloxacin...
2018: Frontiers in Microbiology
Masashi Okamura, Miyuki Kaneko, Shinjiro Ojima, Hiroki Sano, Junji Shindo, Hiroaki Shirafuji, Satomi Yamamoto, Taishi Tanabe, Yasuhiro Yoshikawa, Dong-Liang Hu
Salmonella and Campylobacter cause foodborne enteritis mainly via the consumption of raw/undercooked contaminated poultry meat and products. Broiler flocks are primarily colonized with these bacteria; however, the underlying etiology remains unclear. The present study was conducted in order to obtain further information on the prevalence and genotypic distribution of Salmonella and Campylobacter in free-living crows and broiler flocks in a region for 2 years, thereby facilitating estimations of the potential risk of transmission of C...
March 1, 2018: Microbes and Environments
Sabine Mayer, Rebecca Moeller, João T Monteiro, Kerstin Ellrott, Christine Josenhans, Bernd Lepenies
C-type lectin receptors (CLRs) are carbohydrate-binding receptors that recognize their ligands often in a Ca2+ -dependent manner. Upon ligand binding, myeloid CLRs in innate immunity trigger or inhibit a variety of signaling pathways, thus initiating or modulating effector functions such as cytokine production, phagocytosis, and antigen presentation. CLRs bind to various pathogens, including viruses, fungi, parasites, and bacteria. The bacterium Campylobacter jejuni ( C. jejuni ) is a very frequent Gram-negative zoonotic pathogen of humans, causing severe intestinal symptoms...
2018: Frontiers in Immunology
Mosab A Adam, Ji Wang, Khalid-A Enan, Hongwei Shen, Hao Wang, Abdel R El Hussein, Azza B Musa, Isam M Khidir, Xuejun Ma
Diarrheal disease is a major public health problem for children in developing countries. Knowledge of etiology that causes diarrheal illness is essential to implement public health measures to prevent and control this disease. Published studies regarding the situation of childhood diarrhea in Sudan is scanty. This study aims to investigate viral and bacterial etiology and related clinical and epidemiological factors in children with acute diarrhea in Khartoum State, Sudan. A total of 437 fecal samples were collected from hospitalized children <5 years old with acute diarrhea, viral and bacterial pathogens were investigated by using two-tube multiplex RT-PCR...
2018: Frontiers in Microbiology
Yilmaz Emre Gencay, Martine C H Sørensen, Cory Q Wenzel, Christine M Szymanski, Lone Brøndsted
Campylobacter jejuni NCTC12662 is sensitive to infection by many Campylobacter bacteriophages. Here we used this strain to investigate the molecular mechanism behind phage resistance development when exposed to a single phage and demonstrate how phase variable expression of one surface component influences phage sensitivity against many diverse C. jejuni phages. When C. jejuni NCTC12662 was exposed to phage F207 overnight, 25% of the bacterial cells were able to grow on a lawn of phage F207, suggesting that resistance develops at a high frequency...
2018: Frontiers in Microbiology
F Khattak, V Paschalis, M Green, J G M Houdijk, P Soultanas, J Mahdavi
Reducing Campylobacter spp. carriage in poultry is challenging, but essential to control this major cause of human bacterial gastroenteritis worldwide. Although much is known about the mechanisms and route of Campylobacter spp. colonization in poultry, the literature is scarce on antibiotic-free solutions to combat Campylobacter spp. colonization in poultry. In vitro and in vivo studies were conducted to investigate the role of TYPLEX® Chelate (ferric tyrosine), a novel feed additive, in inhibiting Campylobacter jejuni (C...
February 15, 2018: Poultry Science
Fangzhe Ren, Tianyao Lei, Zhaojun Song, Ting Yu, Qiuchun Li, Jinlin Huang, Xin-An Jiao
The disordered arrangement of flagella biosynthetic genes, combined with a simplified regulatory mechanism, has made elucidating the process of Campylobacter jejuni flagellation difficult. FlhF is a recently identified element that controls the assembly of the flagella, although its function mechanism and regulatory preference are not well defined at present. In this study, we found that inactivation of FlhF caused the transcription of most flagella genes down-regulated. The importance of FlhF was systematically evaluated by analyzing changes in the transcription profiles between wild-type and flhF mutant strains, which showed that FlhF affects late flagella genes obviously...
March 2018: Microbiological Research
Peter F M Teunis, Axel Bonačić Marinović, David R Tribble, Chad K Porter, Arno Swart
Data from a set of different studies on the infectivity and pathogenicity of Campylobacter jejuni were analyzed with a multilevel model, allowing for effects of host species (nonhuman primates and humans) and different strains of the pathogen. All challenge studies involved high doses of the pathogen, resulting in all exposed subjects to become infected. In only one study a dose response effect (increasing trend with dose) for infection was observed. High susceptibility to infection with C. jejuni was found in a joint analysis of outbreaks and challenge studies...
February 8, 2018: Epidemics
Qin Fu, Dejun Liu, Yingyu Wang, Xiaowei Li, Lina Wang, Fugen Yu, Jianzhong Shen, Xi Xia
The metabolome changes of Campylobacter jejuni with resistant gene ermB remain unclear. Here, we described an untargeted metabolomic workflow based on ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry to investigate the metabolites perturbations mediated by ermB in C. jejuni. After optimization of extractants and chromatographic conditions, the combination of 100% methanol extraction with a 12 min gradient by C18 column was adopted for untargeted metabolomic profiling in reversed phase separation...
February 10, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Guillaume Méric, Alan McNally, Alberto Pessia, Evangelos Mourkas, Ben Pascoe, Leonardos Mageiros, Minna Vehkala, Jukka Corander, Samuel K Sheppard
Human infection with the gastrointestinal pathogen C. jejuni is dependent upon the opportunity for zoonotic transmission and the ability of strains to colonize the human host. Certain lineages of this diverse organism are more common in human infection but the factors underlying this overrepresentation are not fully understood. We analysed 601 isolate genomes from agricultural animals and human clinical cases, including isolates from the multi-host (ecological generalist) ST-21 and ST-45 clonal complexes (CCs)...
February 14, 2018: Genome Biology and Evolution
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