keyword
MENU ▼
Read by QxMD icon Read
search

miR-22

keyword
https://www.readbyqxmd.com/read/28927971/the-interaction-of-polymorphisms-in-extracellular-matrix-genes-and-underlying-mirna-motifs-that-modulate-susceptibility-to-anterior-cruciate-ligament-rupture
#1
Kyle Willard, Sasha Mannion, Colleen J Saunders, Malcolm Collins, Alison V September
OBJECTIVES: Variants within genes that encode proteins regulating fibrillogenesis such as BGN (rs1126499 C>T, rs1042103 C>T), COL5A1 (rs12722 C>T) and DCN (rs516115 C>T) have been associated with susceptibility to anterior cruciate ligament (ACL) ruptures. A miRNA mediated transcript instability was proposed for the COL5A1 association. The study aims were: (i) to investigate the association of inferred allele combinations across the COL5A1 3'-UTR, BGN and DCN genes with susceptibility to ACL rupture; and (ii) to use an in silico approach to identify miRNA binding sites common to these risk associated allele combinations...
September 1, 2017: Journal of Science and Medicine in Sport
https://www.readbyqxmd.com/read/28925484/mir-22-inhibits-lung-cancer-cell-emt-and-invasion-through-targeting-snail
#2
K Zhang, X-Y Li, Z-M Wang, Z-F Han, Y-H Zhao
OBJECTIVE: Snail is an important factor in regulating epithelial mesenchymal transition (EMT). Its elevation is related to the enhancement of lung cancer invasion. MicroRNA-22 (MiR-22) plays a role in regulating lung cancer cell invasion. Bioinformatics analysis showed the complementary binding site between miR-22 and Snail. This study aimed to investigate the role of miR-22 in regulating Snail and affecting lung cancer cell invasion and metastasis. MATERIALS AND METHODS: Dual luciferase assay confirmed the targeted relationship between miR-22 and Snail...
August 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28911907/microrna-22-controls-interferon-alpha-production-and-erythroid-maturation-in-response-to-infectious-stress-in-mice
#3
Claudine S Kadmon, Cameron T Landers, Haiyan S Li, Stephanie S Watowich, Antony Rodriguez, Katherine Y King
MicroRNA-22 (miR-22) is a highly conserved microRNA that can regulate cell proliferation, oncogenesis, and cell maturation, especially during stress. In hematopoietic stem cells (HSCs) miR-22 has been reported to be involved in the regulation of key self-renewal factors including Tet2. Recent work demonstrates that miR-22 also participates in regulation of the interferon response, and expression profiling studies suggest that it is variably expressed at different stages in erythroid differentiation. We thus hypothesized that miR-22 regulates maturation of erythroid progenitors during stress hematopoiesis through its interaction with interferon...
September 11, 2017: Experimental Hematology
https://www.readbyqxmd.com/read/28910318/characterization-of-a-novel-panel-of-plasma-micrornas-that-discriminates-between-mycobacterium-tuberculosis-infection-and-healthy-individuals
#4
Jia-Yi Cui, Hong-Wei Liang, Xin-Ling Pan, Di Li, Na Jiao, Yan-Hong Liu, Jin Fu, Xiao-Yu He, Gao-Xiang Sun, Chun-Lei Zhang, Chi-Hao Zhao, Dong-Hai Li, En-Yu Dai, Ke Zen, Feng-Min Zhang, Chen-Yu Zhang, Xi Chen, Hong Ling
Cavities are important in clinical diagnosis of pulmonary tuberculosis (TB) infected by Mycobacterium tuberculosis. Although microRNAs (miRNAs) play a vital role in the regulation of inflammation, the relation between plasma miRNA and pulmonary tuberculosis with cavity remains unknown. In this study, plasma samples were derived from 89 cavitary pulmonary tuberculosis (CP-TB) patients, 89 non-cavitary pulmonary tuberculosis (NCP-TB) patients and 95 healthy controls. Groups were matched for age and gender. In the screening phase, Illumina high-throughput sequencing technology was employed to analyze miRNA profiles in plasma samples pooled from CP-TB patients, NCP-TB patients and healthy controls...
2017: PloS One
https://www.readbyqxmd.com/read/28903345/profiling-of-metastatic-small-intestine-neuroendocrine-tumors-reveals-characteristic-mirnas-detectable-in-plasma
#5
Michaela Bowden, Chensheng W Zhou, Sui Zhang, Lauren Brais, Ashley Rossi, Laurent Naudin, Arunthi Thiagalingam, Ewa Sicinska, Matthew H Kulke
BACKGROUND: Current diagnostic and prognostic blood-based biomarkers for neuroendocrine tumors are limited. MiRNAs have tumor-specific expression patterns, are relatively stable, and can be measured in patient blood specimens. We performed a multi-stage study to identify and validate characteristic circulating miRNAs in patients with metastatic small intestine neuroendocrine tumors, and to assess associations between miRNA levels and survival. METHODS: Using a 742-miRNA panel, we identified candidate miRNAs similarly expressed in 19 small intestine neuroendocrine tumors and matched plasma samples...
August 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28900088/-estimation-of-time-dependent-microrna-expression-patterns-in-brain-tissue-leukocytes-and-blood-plasma-of-rats-under-photochemically-induced-focal-cerebral-ischemia
#6
V A Gusar, A V Timofeeva, I S Zhanin, S I Shram, V G Pinelis
miRNA expression over different time periods (24 and 48 h) using the quantitative RT-PCR and deep sequencing has been evaluated in a model of photochemically induced thrombosis. A combination of two approaches allowed us to determine the miRNA expression patterns caused by ischemia. Nine miRNAs, including let-7f-5p, miR-221-3p, miR-21-5p, miR-30c-5p, miR-30a-3p, miR-223-3p, miR-23a-3p, miR-22-5p, and miR-99a-5p, were differentially expressed in brain tissue and leukocytes of rats 48 h after onset of ischemia...
July 2017: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/28886082/haloperidol-induces-pharmacoepigenetic-response-by-modulating-mirna-expression-global-dna-methylation-and-expression-profiles-of-methylation-maintenance-genes-and-genes-involved-in-neurotransmission-in-neuronal-cells
#7
Babu Swathy, Moinak Banerjee
INTRODUCTION: Haloperidol has been extensively used in various psychiatric conditions. It has also been reported to induce severe side effects. We aimed to evaluate whether haloperidol can influence host methylome, and if so what are the possible mechanisms for it in neuronal cells. Impact on host methylome and miRNAs can have wide spread alterations in gene expression, which might possibly help in understanding how haloperidol may impact treatment response or induce side effects. METHODS: SK-N-SH, a neuroblasoma cell line was treated with haloperidol at 10μm concentration for 24 hours and global DNA methylation was evaluated...
2017: PloS One
https://www.readbyqxmd.com/read/28883618/cg200745-an-hdac-inhibitor-induces-anti-tumour-effects-in-cholangiocarcinoma-cell-lines-via-mirnas-targeting-the-hippo-pathway
#8
Dawoon E Jung, Soo Been Park, Kahee Kim, Chanyang Kim, Si Young Song
Cholangiocarcinoma is a devastating malignancy with fatal complications that exhibits low response and resistance to chemotherapy. Here, we evaluated the anticancer effects of CG200745, a novel histone deacetylase inhibitor, either alone or in combination with standard chemotherapy drugs in cholangiocarcinoma cells. CG200745 dose-dependently reduced the viability of cholangiocarcinoma cells in vitro and decreased tumour volume and weight in a xenograft model. Administering CG200745 along with other chemotherapeutic agents including gemcitabine, 5-fluorouracil (5-FU), cisplatin, oxaliplatin, or gemcitabine plus cisplatin further decreased cholangiocarcinoma cell viability, with a combination index < 1 that indicated synergistic action...
September 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28882183/mir-22-suppresses-tumorigenesis-and-improves-radiosensitivity-of-breast-cancer-cells-by-targeting-sirt1
#9
Xia Zhang, Yuehua Li, Dan Wang, Xiaoer Wei
BACKGROUND: miR-22 has been shown to be frequently downregulated and act as a tumor suppressor in multiple cancers including breast cancers. However, the role of miR-22 in regulating the radioresistance of breast cancer cells, as well as its underlying mechanism is still not well understood. METHODS: The expressions of miR-22 and sirt1 at mRNA and protein levels were examined by qRT-PCR and Western Blot. The effects of miR-22 overexpression and sirt1 knockdown on cell viability, apoptosis, radiosensitivity, γ-H2AX foci formation were evaluated by CCK-8 assay, flow cytometry, colony formation assay, and γ-H2AX foci formation assay, respectively...
September 7, 2017: Biological Research
https://www.readbyqxmd.com/read/28749179/analysis-of-microrna-expression-and-micronuclei-frequency-in-workers-exposed-to-vinyl-chloride-monomer-in-china
#10
Nan-Nan Feng, Yan Fang, Ya-Nan Zhang, Xiao-Wen Xu, Yong Li, Jin-Wei Wang, Yong-Liang Li, Paul Brandt-Rauf, Zhao-Lin Xia
AIM: To identify differently expressed miRNAs associated with vinyl chloride monomer (VCM) and micronuclei (MN) frequency. METHOD: In discovery stage, we used microarray to detect miRNAs expression in peripheral blood lymphocytes between six low and six high VCM-exposed workers grouped by medium cumulative exposure dose. Then we validated four miRNAs using real-time quantitative reverse transcription PCR (qRT-PCR) and detected the micronuclei frequencies using cytokinesis-block micronucleus assay in 94 VCM-exposed workers and 53 healthy control subjects...
July 27, 2017: Epigenomics
https://www.readbyqxmd.com/read/28739418/deficiency-of-dgcr8-increases-bone-formation-through-downregulation-of-mir-22-expression
#11
Young-Jin Choi, Sujin Jeong, Kyung-Ae Yoon, Hye-Jin Sung, Hye-Sim Cho, Dong Wook Kim, Je-Yoel Cho
MicroRNAs (miRNA) significantly contribute to bone formation by post-transcriptional regulation of gene expression. Mature miRNAs are generated following sequential cleavage by DROSHA/DGCR8 and DICER. However, recent studies have identified that some miRNAs require only one of these enzymes. Most studies seeking to clarify the role of miRNA during bone formation have been performed using DICER deletion strategies, but little is known regarding the role of DGCR8. To study the function of DGCR8 in osteogenesis, we generated mice in which Dgcr8 is conditionally deleted in osteoprogenitor cells by Col1a1-Cre...
July 21, 2017: Bone
https://www.readbyqxmd.com/read/28703801/lncrna-miat-functions-as-a-competing-endogenous-rna-to-upregulate-dapk2-by-sponging-mir-22-3p-in-diabetic-cardiomyopathy
#12
Xiang Zhou, Wei Zhang, Mengchao Jin, Jianchang Chen, Weiting Xu, Xiangqing Kong
We previously established a rat model of diabetic cardiomyopathy (DCM) and found that the expression of long non-coding RNA myocardial infarction-associated transcript (MIAT) was significantly upregulated. The present study was aimed to determine the pathologic role of MIAT in the development of DCM. MIAT knockdown was found to reduce cardiomyocyte apoptosis and improve left ventricular function in diabetic rats. High glucose could increase MIAT expression and induce apoptosis in cultured neonatal cardiomyocytes...
July 13, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28688106/human-milk-exosomes-and-their-micrornas-survive-digestion-in-vitro-and-are-taken-up-by-human-intestinal-cells
#13
Yalin Liao, Xiaogu Du, Jie Li, Bo Lönnerdal
SCOPE: Human milk exosomes provide a natural means of genetic material transfer to infants; however, the effect of gastric/pancreatic digestion milk exosomes stability and their microRNA content is largely unknown. METHODS AND RESULTS: We took a simulated gastric/pancreatic digestion protocol to perform in vitro digestion of milk exosomes, explore intestinal epithelial uptake, and further elucidate microRNA responses to digestion at early-, mid-, late lactation by massive parallel sequencing...
July 7, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28675011/-characterization-of-micrornas-profiles-of-induced-pluripotent-stem-cells-reprogrammed-from-human-dental-pulp-stem-cells-and-stem-cells-from-apical-papilla
#14
Tan Xiaobing, Dai Qingyuan
OBJECTIVE: To compare characterization of microRNAs (miRNAs) expression profiles of induced pluripotent stem cells (iPSCs) reprogrammed from human dental pulp stem cells (DPSCs) and stem cells from apical papilla (SCAP) and screen-specific microRNA. METHODS: Human DPSCs and SCAP were reprogrammed into iPSCs using a Sendai virus vector. Total RNA of human DPSCs-iPSCs and SCAP-iPSCs were extracted. miRNAs were labeled and hybridized. Slides were scanned, and images were imported into GenePix Pro 6...
June 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/28653781/differential-expression-profile-of-long-non-coding-rna-in-cardiomyocytes-autophagy-induced-by-angiotensin-ii
#15
Ying Gu, Fan Yang, Ru-Ming Xu, Yun-Yan Zhang, Yang Li, Su-Xuan Liu, Guan-Xin Zhang, Guo-Kun Wang, Li-Ping Ma
Autophagy is a ubiquitous intracellular process for cellular homeostasis maintenance by recycling damaged protein and organelles. Dysregulation of cardiomyocytes autophagic activity is implicated in various heart diseases. Recent studies had demonstrated that long non-coding RNAs (lncRNAs) played crucial roles on modulation of autophagic activity. In this study, we first established an angiotensin II-induced autophagy model on neonatal rat cardiomyocytes. Western blot assay confirmed that the expression of Beclin 1 and the conversion of soluble LC3-I to lipid bound LC3-II were significantly increased at 12 h after angiotensin II stimulation, but the cardiomyocytes surface area and hypertrophic markers expression had no significant change...
June 27, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28637007/c-fos-dependent-mir-22-targets-mdc1-and-regulates-dna-repair-in-terminally-differentiated-cells
#16
Jung-Hee Lee, Seon-Joo Park, Seok Won Kim, Gurusamy Hariharasudhan, Sung-Mi Jung, Semo Jun, Jeongsik Yong, Ho Jin You
Terminally differentiated cells have a reduced capacity to repair double-stranded breaks (DSB) in DNA, however, the underlying molecular mechanism remains unclear. Here, we show that miR-22 is upregulated during postmitotic differentiation of human breast MCF-7 cells, hematopoietic HL60 and K562 cells. Increased expression of miR-22 in differentiated cells was associated with decreased expression of MDC1, a protein that plays a key role in the response to DSBs. This downregulation of MDC1 was accompanied by reduced DSB repair, impaired recruitment of the protein to the site of DNA damage following IR...
July 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28628924/chrysin-alters-micrornas-expression-levels-in-gastric-cancer-cells-possible-molecular-mechanism
#17
Farideh Mohammadian, Younes Pilehvar-Soltanahmadi, Shahriar Alipour, Mehdi Dadashpour, Nosratollah Zarghami
Background Gastric carcinoma still remains the second most common cause of cancer mortality in the world. Chrysin, as a flavone, has showed cancer chemopreventive activity. The cellular and molecular mechanisms of chrysin in cancer cells have not been fully understood. Objective In this study, we investigate expression levels of let-7a, miR-9, mir-18a, miR-21, miR-22, miR-34a, miR-126 and mir-221 to describe the anti-cancer effects of chrysin. Materials and Methods The cytotoxic effects of chrysin were assessed using MTT assay...
September 2017: Drug Research
https://www.readbyqxmd.com/read/28627686/hypoxia-and-acidosis-driven-aberrations-of-secreted-micrornas-in-endometrial-cancer-in-vitro
#18
Julia Eismann, Marc Hirschfeld, Thalia Erbes, Gerta Rücker, Markus Jäger, Andrea Ritter, Daniela Weiss, Gerald Gitsch, Sebastian Mayer
Due to their post-transcriptional regulatory impact on gene expression, microRNAs (miRNA, miRs) influence decisively cellular processes of differentiation, proliferation and apoptosis. In oncogenic pathways various miRNAs exert either oncogenic or tumor suppressor activities in a stage-specific manner. Dysregulation of miRNA expression pattern has been associated with several human cancers including endometrial cancer (EC). In the present study, expression profile alterations of EC associated secreted miRNAs were determined under the microenvironmental stress situations hypoxia and acidosis occurring in tumor progression and metastasis...
June 13, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28591749/microrna-22-can-regulate-expression-of-the-long-non-coding-rna-meg3-in-acute-myeloid-leukemia
#19
Hongxia Yao, Pei Sun, Mengling Duan, Lie Lin, Yanping Pan, Congming Wu, Xiangjun Fu, Hua Wang, Li Guo, Tianbo Jin, Yipeng Ding
AIM: Acute myeloid leukemia (AML) is the most common blood tumor with poor prognosis. At present, the research found that the pathogenesis of AML is related to many factors, such as recurrent somatic mutations and gene expression and epigenetic changes, however, the molecular mechanism of AML is still unclear. Long non-coding RNA MEG3 is a newly found tumor suppressor and plays a very important role in the regulation of a variety of tumor formation and progression. Studies found that the MEG3 expression was significantly decreased in AML...
May 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28548950/enolase-1-stimulates-glycolysis-to-promote-chemoresistance-in-gastric-cancer
#20
Xiaoling Qian, Wenxia Xu, Jinye Xu, Qiqi Shi, Jiaqiu Li, Yu Weng, Zhinong Jiang, Lifeng Feng, Xian Wang, Jianwei Zhou, Hongchuan Jin
Chemotherapy is the major choice for the cancer treatment of early and advanced stages. However, intrinsic or acquired drug resistance significantly restricts the clinical efficacy of chemotherapy. It is critical to develop novel approaches to detect and overcome drug resistance. In this study, we demonstrated that accelerated glycolysis played a pivotal role in both intrinsic and acquired cisplatin-resistance of gastric cancer cells. The metabolic reprogramming of cisplatin-resistant cells was characterized by increased glycolysis dependence...
July 18, 2017: Oncotarget
keyword
keyword
119499
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"