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https://www.readbyqxmd.com/read/28749179/analysis-of-microrna-expression-and-micronuclei-frequency-in-workers-exposed-to-vinyl-chloride-monomer-in-china
#1
Nan-Nan Feng, Yan Fang, Ya-Nan Zhang, Xiao-Wen Xu, Yong Li, Jin-Wei Wang, Yong-Liang Li, Paul Brandt-Rauf, Zhao-Lin Xia
AIM: To identify differently expressed miRNAs associated with vinyl chloride monomer (VCM) and micronuclei (MN) frequency. METHOD: In discovery stage, we used microarray to detect miRNAs expression in peripheral blood lymphocytes between six low and six high VCM-exposed workers grouped by medium cumulative exposure dose. Then we validated four miRNAs using real-time quantitative reverse transcription PCR (qRT-PCR) and detected the micronuclei frequencies using cytokinesis-block micronucleus assay in 94 VCM-exposed workers and 53 healthy control subjects...
July 27, 2017: Epigenomics
https://www.readbyqxmd.com/read/28739418/deficiency-of-dgcr8-increases-bone-formation-through-downregulation-of-mir-22-expression
#2
Young-Jin Choi, Sujin Jeong, Kyung-Ae Yoon, Hye-Jin Sung, Hye-Sim Cho, Dong Wook Kim, Je-Yoel Cho
MicroRNAs (miRNA) significantly contribute to bone formation by post-transcriptional regulation of gene expression. Mature miRNAs are generated following sequential cleavage by DROSHA/DGCR8 and DICER. However, recent studies have identified that some miRNAs require only one of these enzymes. Most studies seeking to clarify the role of miRNA during bone formation have been performed using DICER deletion strategies, but little is known regarding the role of DGCR8. To study the function of DGCR8 in osteogenesis, we generated mice in which Dgcr8 is conditionally deleted in osteoprogenitor cells by Col1a1-Cre...
July 21, 2017: Bone
https://www.readbyqxmd.com/read/28703801/lncrna-miat-functions-as-a-competing-endogenous-rna-to-upregulate-dapk2-by-sponging-mir-22-3p-in-diabetic-cardiomyopathy
#3
Xiang Zhou, Wei Zhang, Mengchao Jin, Jianchang Chen, Weiting Xu, Xiangqing Kong
We previously established a rat model of diabetic cardiomyopathy (DCM) and found that the expression of long non-coding RNA myocardial infarction-associated transcript (MIAT) was significantly upregulated. The present study was aimed to determine the pathologic role of MIAT in the development of DCM. MIAT knockdown was found to reduce cardiomyocyte apoptosis and improve left ventricular function in diabetic rats. High glucose could increase MIAT expression and induce apoptosis in cultured neonatal cardiomyocytes...
July 13, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28688106/human-milk-exosomes-and-their-micrornas-survive-digestion-in-vitro-and-are-taken-up-by-human-intestinal-cells
#4
Yalin Liao, Xiaogu Du, Jie Li, Bo Lönnerdal
SCOPE: Human milk exosomes provide a natural means of genetic material transfer to infants; however, the effect of gastric/pancreatic digestion milk exosomes stability and their microRNA content is largely unknown. METHODS AND RESULTS: We took a simulated gastric/pancreatic digestion protocol to perform in vitro digestion of milk exosomes, explore intestinal epithelial uptake, and further elucidate microRNA responses to digestion at early-, mid-, -late lactation by massive parallel sequencing...
July 7, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28675011/-characterization-of-micrornas-profiles-of-induced-pluripotent-stem-cells-reprogrammed-from-human-dental-pulp-stem-cells-and-stem-cells-from-apical-papilla
#5
Tan Xiaobing, Dai Qingyuan
OBJECTIVE: To compare characterization of microRNAs (miRNAs) expression profiles of induced pluripotent stem cells (iPSCs) reprogrammed from human dental pulp stem cells (DPSCs) and stem cells from apical papilla (SCAP) and screen-specific microRNA. METHODS: Human DPSCs and SCAP were reprogrammed into iPSCs using a Sendai virus vector. Total RNA of human DPSCs-iPSCs and SCAP-iPSCs were extracted. miRNAs were labeled and hybridized. Slides were scanned, and images were imported into GenePix Pro 6...
June 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/28653781/differential-expression-profile-of-long-non-coding-rna-in-cardiomyocytes-autophagy-induced-by-angiotensin-ii
#6
Ying Gu, Fan Yang, Ru-Ming Xu, Yun-Yan Zhang, Yang Li, Su-Xuan Liu, Guan-Xin Zhang, Guo-Kun Wang, Li-Ping Ma
Autophagy is a ubiquitous intracellular process for cellular homeostasis maintainance by recycling damaged protein and organelles. Dysregulation of cardiomyocytes autophagic activity is implicated in various heart diseases. Recent studies had demonstrated long non-coding RNAs (lncRNAs) played crucial roles on modulation of autophagic activity. In this study, we first established an angiotensin II-induced autophagy model on neonatal rat cardiomyocytes. Western blot assay confirmed that the expression of Beclin 1 and the conversion of soluble LC3-I to lipid bound LC3-II were significantly increased at 12h after angiotensin II stimulation, but the cardiomyocytes surface area and hypertrophic markers expression had no significant change...
June 27, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28637007/c-fos-dependent-mir-22-targets-mdc1-and-regulates-dna-repair-in-terminally-differentiated-cells
#7
Jung-Hee Lee, Seon-Joo Park, Seok Won Kim, Gurusamy Hariharasudhan, Sung-Mi Jung, Semo Jun, Jeongsik Yong, Ho Jin You
Terminally differentiated cells have a reduced capacity to repair double-stranded breaks (DSB) in DNA, however, the underlying molecular mechanism remains unclear. Here, we show that miR-22 is upregulated during postmitotic differentiation of human breast MCF-7 cells, hematopoietic HL60 and K562 cells. Increased expression of miR-22 in differentiated cells was associated with decreased expression of MDC1, a protein that plays a key role in the response to DSBs. This downregulation of MDC1 was accompanied by reduced DSB repair, impaired recruitment of the protein to the site of DNA damage following IR...
July 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28628924/chrysin-alters-micrornas-expression-levels-in-gastric-cancer-cells-possible-molecular-mechanism
#8
Farideh Mohammadian, Younes Pilehvar-Soltanahmadi, Shahriar Alipour, Mehdi Dadashpour, Nosratollah Zarghami
Background Gastric carcinoma still remains the second most common cause of cancer mortality in the world. Chrysin, as a flavone, has showed cancer chemopreventive activity. The cellular and molecular mechanisms of chrysin in cancer cells have not been fully understood. Objective In this study, we investigate expression levels of let-7a, miR-9, mir-18a, miR-21, miR-22, miR-34a, miR-126 and mir-221 to describe the anti-cancer effects of chrysin. Materials and Methods The cytotoxic effects of chrysin were assessed using MTT assay...
June 19, 2017: Drug Research
https://www.readbyqxmd.com/read/28627686/hypoxia-and-acidosis-driven-aberrations-of-secreted-micrornas-in-endometrial-cancer-in-vitro
#9
Julia Eismann, Marc Hirschfeld, Thalia Erbes, Gerta Rücker, Markus Jäger, Andrea Ritter, Daniela Weiss, Gerald Gitsch, Sebastian Mayer
Due to their post-transcriptional regulatory impact on gene expression, microRNAs (miRNA, miRs) influence decisively cellular processes of differentiation, proliferation and apoptosis. In oncogenic pathways various miRNAs exert either oncogenic or tumor suppressor activities in a stage-specific manner. Dysregulation of miRNA expression pattern has been associated with several human cancers including endometrial cancer (EC). In the present study, expression profile alterations of EC associated secreted miRNAs were determined under the microenvironmental stress situations hypoxia and acidosis occurring in tumor progression and metastasis...
June 13, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28591749/microrna-22-can-regulate-expression-of-the-long-non-coding-rna-meg3-in-acute-myeloid-leukemia
#10
Hongxia Yao, Pei Sun, Mengling Duan, Lie Lin, Yanping Pan, Congming Wu, Xiangjun Fu, Hua Wang, Li Guo, Tianbo Jin, Yipeng Ding
AIM: Acute myeloid leukemia (AML) is the most common blood tumor with poor prognosis. At present, the research found that the pathogenesis of AML is related to many factors, such as recurrent somatic mutations and gene expression and epigenetic changes, however, the molecular mechanism of AML is still unclear. Long non-coding RNA MEG3 is a newly found tumor suppressor and plays a very important role in the regulation of a variety of tumor formation and progression. Studies found that the MEG3 expression was significantly decreased in AML...
May 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28548950/enolase-1-stimulates-glycolysis-to-promote-chemoresistance-in-gastric-cancer
#11
Xiaoling Qian, Wenxia Xu, Jinye Xu, Qiqi Shi, Jiaqiu Li, Yu Weng, Zhinong Jiang, Lifeng Feng, Xian Wang, Jianwei Zhou, Hongchuan Jin
Chemotherapy is the major choice for the cancer treatment of early and advanced stages. However, intrinsic or acquired drug resistance significantly restricts the clinical efficacy of chemotherapy. It is critical to develop novel approaches to detect and overcome drug resistance. In this study, we demonstrated that accelerated glycolysis played a pivotal role in both intrinsic and acquired cisplatin-resistance of gastric cancer cells. The metabolic reprogramming of cisplatin-resistant cells was characterized by increased glycolysis dependence...
July 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28528333/effect-of-1-25-oh-2d3-on-proliferation-of-fibroblast-like-synoviocytes-and-expressions-of-pro-inflammatory-cytokines-through-regulating-microrna-22-in-a-rat-model-of-rheumatoid-arthritis
#12
Ping Fan, Lan He, Nan Hu, Jing Luo, Jing Zhang, Ling-Fei Mo, Yan-Hua Wang, Dan Pu, Xiao-Hong Lv, Zhi-Ming Hao, Chang-Hai Ding, Wu-Jun Xue, Yang Li
OBJECTIVE: This study aims to investigate the regulatory mechanism of 1,25-(OH)2D3 on the proliferation of fibroblast-like synoviocytes (FLS) and expressions of pro-inflammatory cytokines in rheumatoid arthritis (RA) rats via microRNA-22 (miR-22). METHODS: A rat model of RA was established with a subcutaneous injection of type II collagen. After treated with different concentrations of 1,25-(OH)2D3 the proliferation of FLS was estimated by the MTT method, and the optimal concentration of 1,25-(OH)2D3 was selected for further experiments...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28514744/characterization-of-mouse-serum-exosomal-small-rna-content-the-origins-and-their-roles-in-modulating-inflammatory-response
#13
Xin Zhou, Zinan Jiao, Juling Ji, Shuyuan Li, Xiaodi Huang, Xiaoshuang Lu, Heng Zhao, Jingwen Peng, Xinya Chen, Qiuhong Ji, Yuhua Ji
In the last decade, although studies on exosomal microRNAs (miRNAs) derived from serum and other body fluids have increased dramatically; the contents and biological significance of serum exosomes under normal conditions remain unclear. In the present study, we profiled the small RNA content of mouse serum exosomes (mSEs) using small RNAseq and found that fragments of transfer RNAs (tRNAs) and miRNAs were the two predominant exosomal RNA species, accounting for approximately 60% and 10% of mapped reads, respectively...
June 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/28499456/role-of-micrornas-in-premature-ovarian-insufficiency
#14
REVIEW
Ying Guo, Junyan Sun, Dongmei Lai
Premature ovarian insufficiency (POI) is a typical disorder of amenorrhea lasting for a minimum of 4 months. The typical characteristics comprised of declined estrogen and raised serum concentrations of follicle-stimulating hormone (FSH) in women <40-year-old, primarily originating from iatrogenic factors, karyotypic abnormalities, and genetic factors. However, the etiology of POI remains unknown in approximately 90% of cases. POI could lead to infertility, osteoporosis, cardiovascular disorder, and cognitive dysfunction...
May 12, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28487235/transcriptome-wide-analysis-of-micrornas-in-branchiostoma-belcheri-upon-vibrio-parahemolyticus-infection
#15
Ping Jin, Shengjie Li, Lianjie Sun, Caiyun Lv, Fei Ma
MicroRNAs (miRNAs) are endogenous small non-coding RNAs that participate in diverse biological processes via regulating expressions of target genes at post-transcriptional level. Amphioxus, as modern survivor of an ancient chordate lineage, is a model organism for comparative genomics study. However, miRNAs involved in regulating immune responses in Branchiostoma belcheri are largely unclear. Here, we systematically investigated the microRNAs (miRNAs) involved in regulating immune responses in the cephalochordate amphioxus (Branchiostoma belcheri) through next-generation deep sequencing of amphioxus samples infected with Vibrio parahemolyticus...
May 6, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28482669/evaluation-of-mir-22-and-mir-20a-as-diagnostic-biomarkers-for-gastric-cancer
#16
Zahra Jafarzadeh-Samani, Sareh Sohrabi, Khadije Shirmohammadi, Hosein Effatpanah, Reza Yadegarazari, Massoud Saidijam
BACKGROUND: Gastric cancer (GC) is the fourth most common cancers and the second reason for cancer-related death around the world, particularly in East Asian countries. Diagnosing GC in its early stages is followed by more successful treatment. Unfortunately, there is no accurate method for GC diagnosis in its early stages. Recently, miRNAs have been investigated in the most cancer researches which have demonstrated that they have been dysregulated in many cancers. METHODS: This case-control study aims to investigate the expression rate of miR-22 and miR-20a in 32 cancerous tissues as well as 32 healthy adjacent tissues...
April 2017: Chinese Clinical Oncology
https://www.readbyqxmd.com/read/28471251/microrna-22-3p-as-a-novel-regulator-and-therapeutic-target-for-autoimmune-diseases
#17
Bin Wang, Qiuming Yao, Donghua Xu, Jin-An Zhang
MicroRNAs (miRNAs) are a class of noncoding RNAs and have emerged as critical regulators of gene expression. Some miRNAs play important roles in regulating the function of the immune system and are involved in the pathogenesis of autoimmune diseases. Recent studies suggested that microRNA-22-3p (miR-22-3p) was able to regulate the function of several types of immune cells and may be involved in the development of autoimmune diseases. We systematically reviewed relevant literatures to provide a comprehensive review of the possible roles of miR-22-3p in autoimmune diseases...
May 4, 2017: International Reviews of Immunology
https://www.readbyqxmd.com/read/28469417/plasma-microrna-levels-following-resection-of-metastatic-melanoma
#18
Nicholas Latchana, Zachary B Abrams, J Harrison Howard, Kelly Regan, Naduparambil Jacob, Paolo Fadda, Alicia Terando, Joseph Markowitz, Doreen Agnese, Philip Payne, William E Carson
Melanoma remains the leading cause of skin cancer-related deaths. Surgical resection and adjuvant therapies can result in disease-free intervals for stage III and stage IV disease; however, recurrence is common. Understanding microRNA (miR) dynamics following surgical resection of melanomas is critical to accurately interpret miR changes suggestive of melanoma recurrence. Plasma of 6 patients with stage III (n = 2) and stage IV (n = 4) melanoma was evaluated using the NanoString platform to determine pre- and postsurgical miR expression profiles, enabling analysis of more than 800 miRs simultaneously in 12 samples...
2017: Bioinformatics and Biology Insights
https://www.readbyqxmd.com/read/28454403/mir-22-promotes-apoptosis-of-osteosarcoma-cells-via-inducing-cell-cycle-arrest
#19
Pengzhou Gai, Hongliang Sun, Guangda Wang, Qiang Xu, Xiaojun Qi, Zuofu Zhang, Lei Jiang
To study the effects of miR-22 on the proliferation and the apoptosis of osteosarcoma MG-63 cell line and to explore the potential molecular mechanism that miR-22 regulates this biological process. Quantitive real-time polymerase chain reaction (RT-qPCR) was performed to explore the miRNA level of miR-22. The MG-63 cell line was infected with miR-22 mimics for establishment of miR-22 overexpression. Non-infected cells were in blank group and cells infected with empty vector were served as negative control (NC group)...
April 2017: Oncology Letters
https://www.readbyqxmd.com/read/28446714/profiling-of-metastatic-small-intestine-neuroendocrine-tumors-reveals-characteristic-mirnas-detectable-in-plasma
#20
Michaela Bowden, Chensheng W Zhou, Sui Zhang, Lauren Brais, Ashley Rossi, Laurent Naudin, Arunthi Thiagalingam, Ewa Sicinska, Matthew H Kulke
BACKGROUND: Current diagnostic and prognostic blood-based biomarkers for neuroendocrine tumors are limited. MiRNAs have tumor-specific expression patterns, are relatively stable, and can be measured in patient blood specimens. We performed a multi-stage study to identify and validate characteristic circulating miRNAs in patients with metastatic small intestine neuroendocrine tumors, and to assess associations between miRNA levels and survival. METHODS: Using a 742-miRNA panel, we identified candidate miRNAs similarly expressed in 19 small intestine neuroendocrine tumors and matched plasma samples...
April 7, 2017: Oncotarget
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