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Jatinder Singh, Ramanpreet Shah, Dhandeep Singh
The mast cells are integral part of immune system and they have pleiotropic physiological functions in our body. Any type of abnormal stimuli causes the mast cells receptors to spur the otherwise innocuous mast cells to degranulate and release inflammatory mediators like histamine, cytokines, chemokines and prostaglandins. These mediators are involved in various diseases like allergy, asthma, mastocytosis, cardiovascular disorders, etc. Herein, we describe the receptors involved in degranulation of mast cells and are broadly divided into four categories: G-protein coupled receptors, ligand gated ion channels, immunoreceptors and pattern recognition receptors...
September 29, 2016: International Immunopharmacology
Yifei Qi, Jeffery B Klauda, Wonpil Im
Site-directed spin-labeling electron paramagnetic resonance spectroscopy is a useful tool to obtain information about the environment of specific residues. One of its applications is to investigate membrane protein topology based on the accessibility of the spin label, with the assumption that the position of the spin label in the membrane is close to that of the native residue. This assumption is valid in proteins with well-ordered structures, but could be problematic in small peptides because the labeling may cause a perturbation that is large enough to change local interactions between the peptide and the membrane...
September 27, 2016: Biophysical Journal
K Lapière, K Dhaene, L Matthieu, R Hübner, J Lambert, E Van Marck
Cutaneous T-cell lymphomas represent a group of malignant lymphoproliferative disorders characterised by the occurrence of a monoclonal population of T-lymphocytes. Diagnosis of early stages of this disease is a difficult challenge for both the dermatologist and the dermatopathologist. With the aid of the polymerase chain reaction it is possible to amplify specific regions of the T-cell receptor gamma gene. The amplification products can then be separated by denaturing gradient gel electrophoresis in order to detect a monoclonal population of T-lymphocytes in the infiltrate...
January 1999: Acta Clinica Belgica
Mary-Ellen Taplin, Rana R McKay, Lillian Werner, Elahe A Mostaghel, Rosina T Lis, Olga Voznesensky, Zhenwei Zhang, Brett Marck, Alvin M Matsumoto, Liran Domachevsky, Katherine A Zukotynski, Manoj K Bhasin, Glenn J Bubley, Bruce Montgomery, Philip W Kantoff, Steven P Balk
PURPOSE: Despite the efficacy of abiraterone, a CYP17A1 inhibitor, in metastatic castration-resistant prostate cancer (CRPC), nearly all patients develop resistance. The purpose of this phase II study was to evaluate mechanisms of resistance to more complete androgen synthesis inhibition with abiraterone and dutasteride. EXPERIMENTAL DESIGN: Eligible metastatic CRPC patients underwent a baseline metastasis biopsy. Patients received abiraterone and prednisone for two 4-week cycles...
September 28, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
J Vargova, K Vargova, N Dusilkova, V Kulvait, V Pospisil, J Zavadil, M Trneny, P Klener, T Stopka
No abstract text is available yet for this article.
2016: Blood Cancer Journal
Ibrahima Ka, Papa Saloum Diop, Amadou Bocar Niang, Alioucoly Faye, Jean Marck Ndoye, Babacar Fall
UNLABELLED: Clandestine abortion is known to be a major contributor to maternal mortality. We report a case of a 25-year old patient in her 12th week of amenorrhea with peritonitis due to uterine perforation following abortion, admitted with abdomen and pelvis pain, vomiting and diarrhea. Clinical examination on admission showed asthenic peritonitis. Surgical exploration showed widespread acute peritonitis secondary to a perforation of the uterine dome, with collection of 1500 cc of purulent material, dilated bowel loops and multiple false membranes...
2016: Pan African Medical Journal
Geke Ah van den Elsen, Lieke Tobben, Amir Ia Ahmed, Robbert Jan Verkes, Cornelis Kramers, Radboud M Marijnissen, Marcel Gm Olde Rikkert, Marjolein A van der Marck
Oral tetrahydrocannabinol (THC) is currently studied for its possible efficacy on dementia-related neuropsychiatric symptoms (NPS), but might lead to increased risk of falling. This was a randomised, double-blind, crossover study to evaluate the effects of THC on mobility in dementia patients. Eighteen community-dwelling patients (Mage=77 years) received 1.5 mg of oral THC twice daily and placebo, in random order, for three days, separated by a four-day washout. Balance and gait were assessed using SwayStar(TM) and GAITRite(TM) within two hours after administration, in two consecutive intervention periods, under the following conditions: standing with eyes open (EO) and eyes closed (EC), preferred speed walking with and without a cognitive dual task...
September 13, 2016: Journal of Psychopharmacology
A L Dzierlenga, J D Clarke, N J Cherrington
Interindividual variability in drug response in nonalcoholic steatohepatitis (NASH) can be mediated by altered regulation of drug metabolizing enzymes and transporters. Among these is the mislocalization of multidrug resistance-associated protein (MRP2)/Mrp2 away from the canalicular membrane, which results in decreased transport of MRP2/Mrp2 substrates. The exact mechanism of this mislocalization is unknown, although increased activation of membrane retrieval processes may be one possibility. The current study measures the activation status of various mediators implicated in the active membrane retrieval or insertion of membrane proteins to identify which processes may be important in rodent methionine and choline deficient diet-induced NASH...
November 2016: Drug Metabolism and Disposition: the Biological Fate of Chemicals
George A Jelinek, Alysha M De Livera, Claudia H Marck, Chelsea R Brown, Sandra L Neate, Keryn L Taylor, Tracey J Weiland
OBJECTIVE: Emerging evidence links modifiable lifestyle risk factors to disease progression in multiple sclerosis (MS). We sought further evidence around this hypothesis through detailed analysis of the association with disability of lifestyle behaviours of a large international sample of people with MS. MATERIALS AND METHODS: A total of 2469 people with MS from 57 countries provided self-reported data via cross-sectional online survey on lifestyle (mostly with validated tools) and the primary outcome measure, disability (Patient Determined Disease Steps), categorised from 8 steps into 3 categories, mild, moderate and major disability...
2016: PloS One
Kyota Fujita, Kazumi Motoki, Kazuhiko Tagawa, Xigui Chen, Hiroshi Hama, Kazuyuki Nakajima, Hidenori Homma, Takuya Tamura, Hirohisa Watanabe, Masahisa Katsuno, Chiemi Matsumi, Masunori Kajikawa, Takashi Saito, Takaomi Saido, Gen Sobue, Atsushi Miyawaki, Hitoshi Okazawa
Alzheimer's disease (AD) is the most common neurodegenerative disease, but it remains an intractable condition. Its pathogenesis is predominantly attributed to the aggregation and transmission of two molecules, Aβ and tau; however, other pathological mechanisms are possible. Here, we reveal that phosphorylation of MARCKS, a submembrane protein that regulates the stability of the actin network, occurs at Ser46 prior to aggregation of Aβ and is sustained throughout the course of AD in human and mouse brains...
2016: Scientific Reports
Qi Yin, Shijing Fang, Joungjoa Park, Anne L Crews, Indu Parikh, Kenneth B Adler
Intratracheal (IT) instillation of bacterial lipopolysaccharide (LPS) is a well-established model of acute lung injury (ALI) and/or acute respiratory distress syndrome (ARDS). Since the myristoylated alanine-rich C Kinase substrate (MARCKS) protein is involved in neutrophil migration and proinflammatory cytokine production, we examined whether an aerosolized peptide that inhibits MARCKS function could attenuate LPS - induced lung injury in mice. The peptide, BIO-11006, was delivered at 50 μM via inhalation either just prior to IT instillation of 5μg of LPS into Balb/C mice, or 4, 12, 24 or 36 hrs after LPS instillation...
August 24, 2016: American Journal of Respiratory Cell and Molecular Biology
Daniel Prieto, Flavio R Zolessi
Myristoylated alanin-rich C-kinase substrate (MARCKS) and MARCKS-like 1, each encoded by a different gene, comprise a very small family of actin-modulating proteins with essential roles in mammalian neural development. We show here that four genes (two marcks and two marcksl1) are present in teleosts including zebrafish, while ancient actinopterigians, sarcopterigian fishes, and chondrichtyans only have two. No marcks genes were found in agnaths or invertebrates. All four zebrafish genes are expressed during development, and we show here how their early knockdown causes defects in neural development, with some phenotypical differences...
August 24, 2016: Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
Niels E Franke, Gertjan L Kaspers, Yehuda G Assaraf, Johan van Meerloo, Denise Niewerth, Floortje L Kessler, Pino J Poddighe, Jeroen Kole, Serge J Smeets, Bauke Ylstra, Chonglei Bi, Wee Joo Chng, Terzah M Horton, Rene X Menezes, Renée J P Musters, Sonja Zweegman, Gerrit Jansen, Jacqueline Cloos
PSMB5 mutations and upregulation of the β5 subunit of the proteasome represent key determinants of acquired resistance to the proteasome inhibitor bortezomib (BTZ) in leukemic cells in vitro. We here undertook a multi-modality (DNA, mRNA, miRNA) array-based analysis of human CCRF-CEM leukemia cells and BTZ-resistant subclones to determine whether or not complementary mechanisms contribute to BTZ resistance. These studies revealed signatures of markedly reduced expression of proteolytic stress related genes in drug resistant cells over a broad range of BTZ concentrations along with a high upregulation of myristoylated alanine-rich C-kinase substrate (MARCKS) gene expression...
August 17, 2016: Oncotarget
Marck Htm Haerkens, Wouter van Leeuwen, J Bryan Sexton, Peter Pickkers, Johannes G van der Hoeven
BACKGROUND: As the first objective of caring for patients is to do no harm, patient safety is a priority in delivering clinical care. An essential component of safe care in a clinical department is its safety climate. Safety climate correlates with safety-specific behaviour, injury rates, and accidents. Safety climate in healthcare can be assessed by the Safety Attitudes Questionnaire (SAQ), which provides insight by scoring six dimensions: Teamwork Climate, Job Satisfaction, Safety Climate, Stress Recognition, Working Conditions and Perceptions of Management...
2016: BMC Health Services Research
Adrien Marck, Geoffroy Berthelot, Vincent Foulonneau, Andy Marc, Juliana Antero-Jacquemin, Philippe Noirez, Anne M Bronikowski, Theodore J Morgan, Theodore Garland, Patrick A Carter, Pascal Hersen, Jean-Marc Di Meglio, Jean-François Toussaint
Locomotion is one of the major physiological functions for most animals. Previous studies have described aging mechanisms linked to locomotor performance among different species. However, the precise dynamics of these age-related changes, and their interactions with development and senescence, are largely unknown. Here, we use the same conceptual framework to describe locomotor performances in Caenorhabditis elegans, Mus domesticus, Canis familiaris, Equus caballus, and Homo sapiens We show that locomotion is a consistent biomarker of age-related changes, with an asymmetrical pattern throughout life, regardless of the type of effort or its duration...
August 13, 2016: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
Yi Wen, Robert A Dick, Gerald W Feigenson, Volker M Vogt
UNLABELLED: The retroviral structural protein Gag binds to the inner leaflet of the plasma membrane (PM), and many cellular proteins do so as well. We used Rous sarcoma virus (RSV) Gag together with membrane sensors to study the principles governing peripheral protein membrane binding, including electrostatics, specific recognition of phospholipid headgroups, sensitivity to phospholipid acyl chain compositions, preference for membrane order, and protein multimerization. We used an in vitro liposome-pelleting assay to test protein membrane binding properties of Gag, the well-characterized MARCKS peptide, a series of fluorescent electrostatic sensor proteins (mNG-KRn), and the specific phosphatidylserine (PS) binding protein Evectin2...
October 15, 2016: Journal of Virology
Masahiro Ohsawa, Kei-Ichiro Ishikura, Junpei Mutoh, Hiroaki Hisa
Small molecular G-protein plays a key role in several diseases. This study was designed to reveal the role of RhoA signaling in the pathophysiology of neuropathic pain in mice. Partial sciatic nerve injury caused thermal hyperalgesia, mechanical allodynia, and increased plasma membrane translocation of RhoA in the lumber spinal cord. GFAP-immunoreactivity (ir), Iba-1-ir, and Rho kinase 2 (ROCK2-ir) was also increased in the ipsilateral spinal dorsal horn of nerve-ligated mice. Moreover, partial nerve ligation increased the expression of phosphorylated myristoylated alanine-rich protein kinase C substrate (MARCKS)-ir in the ipsilateral spinal dorsal horn...
October 1, 2016: Neuroscience
Geoffroy Berthelot, Adrien Marck, Vincent Foulonneau, Juliana Antero-Jacquemin, Philippe Noirez, Anne M Bronikowski, Ted J Morgan, Theodore Garland, Patrick A Carter, Jean-François Toussaint
No abstract text is available yet for this article.
May 2016: Medicine and Science in Sports and Exercise
Anta Ngkelo, Adèle Richart, Jonathan A Kirk, Philippe Bonnin, Jose Vilar, Mathilde Lemitre, Pauline Marck, Maxime Branchereau, Sylvain Le Gall, Nisa Renault, Coralie Guerin, Mark J Ranek, Anaïs Kervadec, Luca Danelli, Gregory Gautier, Ulrich Blank, Pierre Launay, Eric Camerer, Patrick Bruneval, Philippe Menasche, Christophe Heymes, Elodie Luche, Louis Casteilla, Béatrice Cousin, Hans-Reimer Rodewald, David A Kass, Jean-Sébastien Silvestre
Acute myocardial infarction (MI) is a severe ischemic disease responsible for heart failure and sudden death. Inflammatory cells orchestrate postischemic cardiac remodeling after MI. Studies using mice with defective mast/stem cell growth factor receptor c-Kit have suggested key roles for mast cells (MCs) in postischemic cardiac remodeling. Because c-Kit mutations affect multiple cell types of both immune and nonimmune origin, we addressed the impact of MCs on cardiac function after MI, using the c-Kit-independent MC-deficient (Cpa3(Cre/+)) mice...
June 27, 2016: Journal of Experimental Medicine
Cuong Van Dao, Md Zahorul Islam, Kasumi Sudo, Mitsuya Shiraishi, Atsushi Miyamoto
Methylmercury (MeHg) is a persistent environmental contaminant that has been reported worldwide. MeHg exposure has been reported to lead to increased risk of cardiovascular diseases; however, the mechanisms underlying the toxic effects of MeHg on the cardiovascular system have not been well elucidated. We have previously reported that mice exposed to MeHg had increased blood pressure along with impaired endothelium-dependent vasodilation. In this study, we investigated the toxic effects of MeHg on a human endothelial cell line, EA...
June 24, 2016: Journal of Veterinary Medical Science
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