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Streptomyces hygroscopicus

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https://www.readbyqxmd.com/read/27909940/enhancement-of-rapamycin-production-by-metabolic-engineering-in-streptomyces-hygroscopicus-based-on-genome-scale-metabolic-model
#1
Lanqing Dang, Jiao Liu, Cheng Wang, Huanhuan Liu, Jianping Wen
Rapamycin, as a macrocyclic polyketide with immunosuppressive, antifungal, and anti-tumor activity produced by Streptomyces hygroscopicus, is receiving considerable attention for its significant contribution in medical field. However, the production capacity of the wild strain is very low. Hereby, a computational guided engineering approach was proposed to improve the capability of rapamycin production. First, a genome-scale metabolic model of Streptomyces hygroscopicus ATCC 29253 was constructed based on its annotated genome and biochemical information...
December 1, 2016: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27903647/the-production-and-utilization-of-gdp-glucose-in-the-biosynthesis-of-trehalose-6-phosphate-by-streptomyces-venezuelae
#2
Matías D Asencion Diez, Farzana Miah, Clare E M Stevenson, David M Lawson, Alberto A Iglesias, Stephen Bornemann
Trehalose-6-phosphate synthase OtsA from streptomycetes is unusual in that it uses GDP-glucose as the donor substrate rather than the more commonly used UDP-glucose. We now confirm that OtsA from Streptomyces venezuelae has such a preference for GDP-glucose and can utilise ADP-glucose to some extent too. A crystal structure of the enzyme shows that it shares twin Rossmann-like domains with the UDP-glucose-specific OtsA from Escherichia coli However, it is structurally more similar to Streptomyces hygroscopicus VldE, a GDP-valienol-dependent pseudo-glycosyltransferase enzyme...
November 30, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27902297/genome-analysis-based-reclassification-of-streptomyces-endus-and-streptomyces-sporocinereus-as-later-heterotypic-synonyms-of-streptomyces-hygroscopicus-subsp-hygroscopicus
#3
Hisayuki Komaki, Natsuko Ichikawa, Akio Oguchi, Moriyuki Hamada, Tomohiko Tamura, Nobuyuki Fujita, Ken-Ichiro Suzuki
The aim of this study is to reclarify the taxonomic relationship among Streptomyces hygroscopicus subsp. hygroscopicus, Streptomyces endus and Streptomyces sporocinereus. Whole genome shotgun sequencing was performed for the type strains of these three taxa. Average nucleotide identity and digital DNA-DNA hybridization values among the three taxa were greater than the thresholds for bacterial species delineation, indicating that they belong to the same genomospecies. In addition, their phenotypic data previously reported also support the synonymy...
October 28, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27869289/integration-of-parallel-13-c-labeling-experiments-and-in-silico-pathway-analysis-for-enhanced-production-of-ascomycin
#4
Haishan Qi, Mengmeng Lv, Kejing Song, Jianping Wen
Herein, the hyper-producing strain for ascomycin was engineered based on (13) C-labeling experiments and elementary flux modes analysis (EFMA). First, the metabolism of non-model organism Streptomyces hygroscopicus var. ascomyceticus SA68 was investigated and an updated network model was reconstructed using (13) C- metabolic flux analysis. Based on the precise model, EFMA was further employed to predict genetic targets for higher ascomycin production. Chorismatase (FkbO) and pyruvate carboxylase (Pyc) were predicted as the promising overexpression and deletion targets, respectively...
November 21, 2016: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/27800124/draft-genome-sequence-of-streptomyces-sp-tp-a0867-an-alchivemycin-producer
#5
Hisayuki Komaki, Natsuko Ichikawa, Akio Oguchi, Moriyuki Hamada, Enjuro Harunari, Shinya Kodani, Nobuyuki Fujita, Yasuhiro Igarashi
Streptomyces sp. TP-A0867 (=NBRC 109436) produces structurally complex polyketides designated alchivemycins A and B. Here, we report the draft genome sequence of this strain together with features of the organism and assembly, annotation, and analysis of the genome sequence. The 9.9 Mb genome of Streptomyces sp. TP-A0867 encodes 8,385 putative ORFs, of which 7,232 were assigned with COG categories. We successfully identified a hybrid polyketide synthase (PKS)/ nonribosomal peptide synthetase (NRPS) gene cluster that could be responsible for alchivemycin biosynthesis, and propose the biosynthetic pathway...
2016: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/27793152/improving-the-active-expression-of-transglutaminase-in-streptomyces-lividans-by-promoter-engineering-and-codon-optimization
#6
Song Liu, Miao Wang, Guocheng Du, Jian Chen
BACKGROUND: Transglutaminases (TGase), which are synthesized as a zymogen (pro-TGase) in Streptomyces sp., are important enzymes in the food industry. Because this pro-peptide is essential for the correct folding of Streptomyces TGase, TGase is usually expressed in an inactive pro-TGase form, which is then converted to active TGase by the addition of activating proteases in vitro. In this study, Streptomyces hygroscopicus TGase was actively produced by Streptomyces lividans through promoter engineering and codon optimization...
October 28, 2016: BMC Biotechnology
https://www.readbyqxmd.com/read/27757508/characterization-of-the-biochemical-physiological-and-medicinal-properties-of-streptomyces-hygroscopicus-actms-9h-isolated-from-the-amazon-brazil
#7
Sandrine M A Lima, Janaína G S Melo, Gardênia C G Militão, Gláucia M S Lima, Maria do Carmo A Lima, Jaciana S Aguiar, Renata M Araújo, Raimundo Braz-Filho, Pascal Marchand, Janete M Araújo, Teresinha G Silva
Actinomycetes are known to produce numerous secondary bioactive metabolites of pharmaceutical interest. The purpose of this study was to isolate, characterize, and investigate the antibacterial, antifungal, and anticancer activities of metabolites produced by Actinobacteria isolated from the rhizosphere of Paullinia cupana. The Actinobacteria was identified as Streptomyces hygroscopicus ACTMS-9H. Based on a bioguided study, the methanolic biomass extract obtained from submerged cultivation had the most potent antibacterial, antifungal, and cytotoxic activities...
October 18, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27514663/significance-of-oxygen-carriers-and-role-of-liquid-paraffin-in-improving-validamycin-a-production
#8
Jinsong Feng, Jing Jiang, Yan Liu, Wei Li, Ramila Azat, Xiaodong Zheng, Wen-Wen Zhou
Validamycin A (Val-A) synthesized by Streptomyces hygroscopicus 5008 is widely used as a high-efficient antibiotic to protect plants from sheath blight disease. A novel fermentation strategy was introduced to stimulate Val-A production by adding oxygen carriers. About 58 % increase in Val-A production was achieved using liquid paraffin. Further, biomass, carbon source, metabolic genes, and metabolic enzymes were studied. It was also found that the supplementation of liquid paraffin increased the medium dissolved oxygen and intracellular oxidative stress level...
October 2016: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27296090/novel-spectrophotometric-approach-for-determination-of-validamycin-a-in-fermentation-of-streptomyces-hygroscopicus
#9
Wei Li, Jinsong Feng, Yan Liu, Jing Jiang, Xiaodong Zheng, Wen-Wen Zhou
Validamycin A (Val-A), produced by Streptomyces hygroscopicus 5008 in industrial fermentation, is one of the most widely used anti-fungal agro-antibiotics in Asia and high performance liquid chromatography (HPLC) assay is usually used to determine the production of Val-A. A new approach to determine Val-A by spectrophotometer is developed. During the fermentation of S. hygroscopicus 5008, a pigment secretion was found along with the Val-A biosynthesis. There was a stable relationship between the concentration of Val-A and spectral absorption (SA) value of this pigment at 450 nm, even in different fermentation cultures or conditions...
June 10, 2016: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/27198007/draft-genome-sequence-of-streptomyces-hygroscopicus-subsp-hygroscopicus-nbrc-16556
#10
Hisayuki Komaki, Natsuko Ichikawa, Akio Oguchi, Moriyuki Hamada, Tomohiko Tamura, Ken-Ichiro Suzuki, Nobuyuki Fujita
Here, we report the draft genome sequence of strain NBRC 16556, deposited as Streptomyces hygroscopicus subsp. hygroscopicus into the NBRC culture collection. An average nucleotide identity analysis confirmed that the taxonomic identification is correct. The genome sequence will serve as a valuable reference for genome mining to search new secondary metabolites.
2016: Genome Announcements
https://www.readbyqxmd.com/read/27187382/sirolimus-and-everolimus-pathway-reviewing-candidate-genes-influencing-their-intracellular-effects
#11
REVIEW
Simona Granata, Alessandra Dalla Gassa, Amedeo Carraro, Matteo Brunelli, Giovanni Stallone, Antonio Lupo, Gianluigi Zaza
Sirolimus (SRL) and everolimus (EVR) are mammalian targets of rapamycin inhibitors (mTOR-I) largely employed in renal transplantation and oncology as immunosuppressive/antiproliferative agents. SRL was the first mTOR-I produced by the bacterium Streptomyces hygroscopicus and approved for several medical purposes. EVR, derived from SRL, contains a 2-hydroxy-ethyl chain in the 40th position that makes the drug more hydrophilic than SRL and increases oral bioavailability. Their main mechanism of action is the inhibition of the mTOR complex 1 and the regulation of factors involved in a several crucial cellular functions including: protein synthesis, regulation of angiogenesis, lipid biosynthesis, mitochondrial biogenesis and function, cell cycle, and autophagy...
2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27105915/analysis-of-pupylation-of-streptomyces-hygroscopicus-5008-in%C3%A2-vitro
#12
Xibing Xu, Yulong Niu, Ke Liang, Guomin Shen, Qing Cao, Yi Yang
Prokaryotic ubiquitin-like protein (Pup) is a post-translational modifier that can be attached to substrate proteins in Actinobacteria. The modification process is defined as pupylation and is associated with proteasome-mediated protein degradation in mycobacteria and streptomycetes. Here, we report the pupylation of Streptomyces hygroscopicus 5008 in vitro. Each component of the Pup system was expressed in Escherichia coli and poly-Pup chains were observed by western blot analysis. Though only one potential Pup substrate (SHJG_3659) was identified using MS/MS, we verified this candidate and other predicted substrates by a reconstituted Pup system in E...
May 20, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/26988280/new-c-19-modified-geldanamycin-derivatives-synthesis-antitumor-activities-and-physical-properties-study
#13
Yu-Feng Liu, Jing-Jing Zhong, Ling Lin, Juan-Juan Liu, Yi-Guang Wang, Wei-Qing He, Zhao-Yong Yang
Thiazinogeldanamycin (2) was identified from Streptomyces hygroscopicus 17997 at the late stage of the fermentation. The pH was firstly proposed as an important factor in the biosynthesis of it. It was verified that 2 was produced by direct chemical reactions between geldanamycin (1, GDM) and cysteine or aminoethanethiol hydrochloride at pH > 7 in vitro. The proposed synthesis pathway for compound 2 was also discussed. Eleven new C-19-modified GDM derivatives, including five stable hydroquinone form derivatives, were synthesized, most of which exhibited desirable properties such as lower cytotoxicity, increased water solubility, and potent antitumor activity...
August 2016: Journal of Asian Natural Products Research
https://www.readbyqxmd.com/read/26704727/complete-genome-of-streptomyces-hygroscopicus-subsp-limoneus-kctc-1717-kccm-11405-a-soil-bacterium-producing-validamycin-and-diverse-secondary-metabolites
#14
Sang-Heon Lee, Hanna Choe, Kyung Sook Bae, Doo-Sang Park, Arshan Nasir, Kyung Mo Kim
Streptomyces hygroscopicus subsp. limoneus is a Gram-positive, aerobic, aerial mycelial, spore-forming bacterium that was first isolated from a soil sample in Akashi City, Hyogo Prefecture, Japan. We here report the complete genome of S. hygroscopicus subsp. limoneus KCTC 1717 (=KCCM 11405=IFO 12704=ATCC 21432), which consists of 10,537,932 bp (G+C content of 71.96%) with two linear chromosomes, 8983 protein-coding genes, 67 tRNAs and 6 rRNA operons. Genes related to biosynthesis of validamycin, valienamine and diverse secondary metabolites were detected in this genome...
February 10, 2016: Journal of Biotechnology
https://www.readbyqxmd.com/read/26539052/antagonistic-effect-of-streptomyces-sp-bs062-against-botrytis-diseases
#15
Young-Sook Kim, In-Kyoung Lee, Bong-Sik Yun
The use of microorganisms and their secreted molecules to prevent plant diseases is considered an attractive alternative and way to supplement synthetic fungicides for the management of plant diseases. Strain BS062 was selected based on its ability to inhibit the mycelial growth of Botrytis cinerea, a major causal fungus of postharvest root rot of ginseng and strawberry gray mold disease. Strain BS062 was found to be closely related to Streptomyces hygroscopicus (99% similarity) on the basis of 16S ribosomal DNA sequence analysis...
September 2015: Mycobiology
https://www.readbyqxmd.com/read/26448987/-storage-of-actinobacteria-of-the-genera-streptomyces-and-nonomuraea-by-low-temperature-preservation
#16
O N Sineva, N G Kulikova, S N Filippova, L P Terekhova
The influence of storage of actinobacteria Streptomyces hygroscopicus RIA 1433T, Nonomuraea roseoviolacea subsp. carminata INA 4281 and Nonomuraea sp. INA 34-06 at extremely low temperatures (-70 degrees C) for 1.5 years was studied with respect to their viability and antibiotic activity. The spores of the actinobacteria preserved their high viability when freezed at a concentration of 10(5)-10(7) CFU/ml. As for the antibiotic activity against the test culture Micrococcus luteus ATCC 9341, the strains differed: the S...
2014: Antibiotiki i Khimioterapii︠a︡, Antibiotics and Chemoterapy [sic]
https://www.readbyqxmd.com/read/26436873/de-novo-biosynthesis-of-%C3%AE-valienamine-in-engineered-streptomyces-hygroscopicus-5008
#17
Li Cui, Ying Zhu, Xiaoqing Guan, Zixin Deng, Linquan Bai, Yan Feng
The C7N aminocyclitol β-valienamine is a lead compound for the development of new biologically active β-glycosidase inhibitors as chemical chaperone therapeutic agents for lysosomal storage diseases. Its chemical synthesis is challenging due to the presence of multichiral centers in the structure. Herein, we took advantage of a heterogeneous aminotransferase with stereospecificity and designed a novel pathway for producing β-valienamine in Streptomyces hygroscopicus 5008, a validamycin producer. The aminotransferase BtrR from Bacillus circulans was able to convert valienone to β-valienamine with an optical purity of up to >99...
January 15, 2016: ACS Synthetic Biology
https://www.readbyqxmd.com/read/26328935/conserved-biosynthetic-pathways-for-phosalacine-bialaphos-and-newly-discovered-phosphonic-acid-natural-products
#18
Joshua A V Blodgett, Jun Kai Zhang, Xiaomin Yu, William W Metcalf
Natural products containing phosphonic or phosphinic acid functionalities often display potent biological activities with applications in medicine and agriculture. The herbicide phosphinothricin-tripeptide (PTT) was the first phosphinate natural product discovered, yet despite numerous studies, questions remain surrounding key transformations required for its biosynthesis. In particular, the enzymology required to convert phosphonoformate to carboxyphosphonoenolpyruvate and the mechanisms underlying phosphorus methylation remain poorly understood...
January 2016: Journal of Antibiotics
https://www.readbyqxmd.com/read/26323360/17-demethoxy-reblastatin-an-hsp90-inhibitor-induces-mitochondria-mediated-apoptosis-through-downregulation-of-mcl-1-in-human-hepatocellular-carcinoma-cells
#19
Surong Zhao, Hongmei Li, Chenchen Jiang, Tao Ma, Chengzhu Wu, Qiang Huo, Hao Liu
Heat shock protein 90 (Hsp90) is an attractive therapeutic target. Geldanamycin (GA), the first identified Hsp90 inhibitor, exhibited potent antitumor activity, but possessed significant hepatotoxicity. To overcome the hepatotoxicity derived from the quinone structure of GA, a non-quinone GA derivative 17-demethoxy-reblastatin (17-DR) was obtained from a genetically modified strain of Streptomyces hygroscopicus. In the present study, we examined the anticancer effects of 17-DR on human hepatocellular carcinoma (HCC) cell lines HepG2 and SMMC7721, and its underlying mechanisms...
October 2015: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/26272462/overproduction-of-pro-transglutaminase-from-streptomyces-hygroscopicus-in-yarrowia-lipolytica-and-its-biochemical-characterization
#20
Song Liu, Dan Wan, Miao Wang, Catherine Madzak, Guocheng Du, Jian Chen
BACKGROUND: Transglutaminases (TGase), synthesized as a zymogen (pro-TGase) in Streptomyces sp., are important enzymes in food industry. Due to the important applications of TGase in food industry, obtaining robust and food-safe TGase-producing strains has attracted much attention during the past decade. In this study, Streptomyces hygroscopicus pro-TGase was efficiently expressed and secreted by a food-grade host, Yarrowia lipolytica, without antibiotic markers. RESULTS: The pro-TGase gene was cloned into integrative vectors pINA1296 (monocopy) and pINA1297 (multicopy), and was used to transform the Y...
2015: BMC Biotechnology
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