Danial Ahwazi, Katyayanee Neopane, Greg R Markby, Franziska Kopietz, Ashley J Ovens, Morten Dall, Anna S Hassing, Pamina Gräsle, Yazeed Alshuweishi, Jonas T Treebak, Ian P Salt, Olga Göransson, Elton Zeqiraj, John W Scott, Kei Sakamoto
SBI-0206965, originally identified as an inhibitor of the autophagy initiator kinase ULK1, has recently been reported as a more potent and selective AMP-activated protein kinase (AMPK) inhibitor relative to the widely used, but promiscuous inhibitor Compound C/Dorsomorphin. Here, we studied the effects of SBI-0206965 on AMPK signalling and metabolic readouts in multiple cell types, including hepatocytes, skeletal muscle cells and adipocytes. We observed SBI-0206965 dose dependently attenuated AMPK activator (991)-stimulated ACC phosphorylation and inhibition of lipogenesis in hepatocytes...
August 13, 2021: Biochemical Journal