Ana Rita Soares Mateus, Carmen Crisafulli, Silvia Cruz Barros, Angelina Pena, Ana Sanches Silva
A method was developed for the determination of tropane alkaloids (TAs), including atropine, scopolamine, anisodamine and homatropine in buckwheat and related products. This work presents an optimised methodology based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) extraction procedure followed by ultra-high performance liquid chromatography combined with time-of-flight mass spectrometry for the determination of TAs (atropine, scopolamine, anisodamine and homatropine) in buckwheat samples. The analytical methodology was successfully validated, demonstrating good linearity, low limit of quantification, repeatability (RSDr < 15%), inter-day precision (RSDR < 19%) and recovery (74-113%)...
April 18, 2024: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment