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Peptostreptococcus stomatis

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https://www.readbyqxmd.com/read/27737357/bacterial-diversity-of-symptomatic-primary-endodontic-infection-by-clonal-analysis
#1
Letícia Maria Menezes Nóbrega, Francisco Montagner, Adriana Costa Ribeiro, Márcia Alves Pinto Mayer, Brenda Paula Figueiredo de Almeida Gomes
The aim of this study was to explore the bacterial diversity of 10 root canals with acute apical abscess using clonal analysis. Samples were collected from 10 patients and submitted to bacterial DNA isolation, 16S rRNA gene amplification, cloning, and sequencing. A bacterial genomic library was constructed and bacterial diversity was estimated. The mean number of taxa per canal was 15, ranging from 11 to 21. A total of 689 clones were analyzed and 76 phylotypes identified, of which 47 (61.84%) were different species and 29 (38...
October 10, 2016: Brazilian Oral Research
https://www.readbyqxmd.com/read/27224567/molecular-identification-of-cultivable-bacteria-from-infected-root-canals-associated-with-acute-apical-abscess
#2
Letícia M M Nóbrega, Francisco Montagner, Adriana C Ribeiro, Márcia A P Mayer, Brenda P F A Gomes
The objective of this study was to investigate the bacterial composition present in root canals of teeth associated with acute apical abscess by molecular identification (16S rRNA) of cultivable bacteria. Two hundred and twenty strains isolated by culture from 20 root canals were subjected to DNA extraction and amplification of the 16S rRNA gene (PCR), followed by sequencing. The resulting nucleotide sequences were compared to the GenBank database from the National Center of Biotechnology Information through BLAST...
May 2016: Brazilian Dental Journal
https://www.readbyqxmd.com/read/27080513/tongue-coating-and-the-salivary-microbial-communities-vary-in-children-with-halitosis
#3
Wen Ren, Zhe Xun, Zicheng Wang, Qun Zhang, Xuenan Liu, Hui Zheng, Qian Zhang, Yifei Zhang, Linshuang Zhang, Chunyan Wu, Shuguo Zheng, Nan Qin, S Dusko Ehrlich, Yihong Li, Xuesong He, Tao Xu, Ting Chen, Feng Chen
Halitosis is a common symptom mainly caused by microbial activities in the oral cavity. Here, we used 16S rRNA gene pyrosequencing and metagenomic sequencing to examine oral microbial compositions and their functional variations in children with halitosis. We found that the tongue coating of subjects with halitosis had greater bacterial richness than those of healthy subjects. The relative abundance and prevalence of Leptotrichia wadei and Peptostreptococcus stomatis were higher in tongue coating samples from children with halitosis than those from children without halitosis; Prevotella shahii had higher relative abundance and prevalence in saliva samples from children with halitosis...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27056827/microbiota-based-model-improves-the-sensitivity-of-fecal-immunochemical-test-for-detecting-colonic-lesions
#4
Nielson T Baxter, Mack T Ruffin, Mary A M Rogers, Patrick D Schloss
BACKGROUND: Colorectal cancer (CRC) is the second leading cause of death among cancers in the United States. Although individuals diagnosed early have a greater than 90% chance of survival, more than one-third of individuals do not adhere to screening recommendations partly because the standard diagnostics, colonoscopy and sigmoidoscopy, are expensive and invasive. Thus, there is a great need to improve the sensitivity of non-invasive tests to detect early stage cancers and adenomas. Numerous studies have identified shifts in the composition of the gut microbiota associated with the progression of CRC, suggesting that the gut microbiota may represent a reservoir of biomarkers that would complement existing non-invasive methods such as the widely used fecal immunochemical test (FIT)...
2016: Genome Medicine
https://www.readbyqxmd.com/read/26521147/microbiomes-of-endodontic-periodontal-lesions-before-and-after-chemomechanical-preparation
#5
Brenda P F A Gomes, Vanessa B Berber, Alexis S Kokaras, Tsute Chen, Bruce J Paster
INTRODUCTION: This study was conducted to evaluate the microbiomes of endodontic-periodontal lesions before and after chemomechanical preparation (CMP). METHODS: Clinical samples were taken from 15 root canals (RCs) with necrotic pulp tissues and from their associated periodontal pockets (PPs) (n = 15) of teeth with endodontic-periodontal lesions before and after CMP. The Human Oral Microbe Identification using Next Generation Sequencing (NGS) protocol and viable culture were used to analyze samples from RCs and PPs...
December 2015: Journal of Endodontics
https://www.readbyqxmd.com/read/26408641/metagenomic-analysis-of-faecal-microbiome-as-a-tool-towards-targeted-non-invasive-biomarkers-for-colorectal-cancer
#6
Jun Yu, Qiang Feng, Sunny Hei Wong, Dongya Zhang, Qiao Yi Liang, Youwen Qin, Longqing Tang, Hui Zhao, Jan Stenvang, Yanli Li, Xiaokai Wang, Xiaoqiang Xu, Ning Chen, William Ka Kei Wu, Jumana Al-Aama, Hans Jørgen Nielsen, Pia Kiilerich, Benjamin Anderschou Holbech Jensen, Tung On Yau, Zhou Lan, Huijue Jia, Junhua Li, Liang Xiao, Thomas Yuen Tung Lam, Siew Chien Ng, Alfred Sze-Lok Cheng, Vincent Wai-Sun Wong, Francis Ka Leung Chan, Xun Xu, Huanming Yang, Lise Madsen, Christian Datz, Herbert Tilg, Jian Wang, Nils Brünner, Karsten Kristiansen, Manimozhiyan Arumugam, Joseph Jao-Yiu Sung, Jun Wang
OBJECTIVE: To evaluate the potential for diagnosing colorectal cancer (CRC) from faecal metagenomes. DESIGN: We performed metagenome-wide association studies on faecal samples from 74 patients with CRC and 54 controls from China, and validated the results in 16 patients and 24 controls from Denmark. We further validated the biomarkers in two published cohorts from France and Austria. Finally, we employed targeted quantitative PCR (qPCR) assays to evaluate diagnostic potential of selected biomarkers in an independent Chinese cohort of 47 patients and 109 controls...
September 25, 2015: Gut
https://www.readbyqxmd.com/read/25880819/development-and-pyrosequencing-analysis-of-an-in-vitro-oral-biofilm-model
#7
James O Kistler, Manuel Pesaro, William G Wade
BACKGROUND: Dental caries and periodontal disease are the commonest bacterial diseases of man and can result in tooth loss. The principal method of prevention is the mechanical removal of dental plaque augmented by active agents incorporated into toothpastes and mouthrinses. In-vitro assays that include complex oral bacterial biofilms are required to accurately predict the efficacy of novel active agents in vivo. The aim of this study was to develop an oral biofilm model using the Calgary biofilm device (CBD) seeded with a natural saliva inoculum and analysed by next generation sequencing...
2015: BMC Microbiology
https://www.readbyqxmd.com/read/25777006/development-and-pyrosequencing-analysis-of-an-in-vitro-oral-biofilm-model
#8
James O Kistler, Manuel Pesaro, William G Wade
BACKGROUND: Dental caries and periodontal disease are the commonest bacterial diseases of man and can result in tooth loss. The principal method of prevention is the mechanical removal of dental plaque augmented by active agents incorporated into toothpastes and mouthrinses. In-vitro assays that include complex oral bacterial biofilms are required to accurately predict the efficacy of novel active agents in vivo. The aim of this study was to develop an oral biofilm model using the Calgary biofilm device (CBD) seeded with a natural saliva inoculum and analysed by next generation sequencing...
December 2015: BMC Microbiology
https://www.readbyqxmd.com/read/22817758/comparison-of-oral-microbiota-in-tumor-and-non-tumor-tissues-of-patients-with-oral-squamous-cell-carcinoma
#9
COMPARATIVE STUDY
Smruti Pushalkar, Xiaojie Ji, Yihong Li, Cherry Estilo, Ramanathan Yegnanarayana, Bhuvanesh Singh, Xin Li, Deepak Saxena
BACKGROUND: Bacterial infections have been linked to malignancies due to their ability to induce chronic inflammation. We investigated the association of oral bacteria in oral squamous cell carcinoma (OSCC/tumor) tissues and compared with adjacent non-tumor mucosa sampled 5 cm distant from the same patient (n = 10). By using culture-independent 16S rRNA approaches, denaturing gradient gel electrophoresis (DGGE) and cloning and sequencing, we assessed the total bacterial diversity in these clinical samples...
2012: BMC Microbiology
https://www.readbyqxmd.com/read/21523229/analysis-of-microbiota-associated-with-peri-implantitis-using-16s-rrna-gene-clone-library
#10
Tatsuro Koyanagi, Mitsuo Sakamoto, Yasuo Takeuchi, Moriya Ohkuma, Yuichi Izumi
BACKGROUND: Peri-implantitis (PI) is an inflammatory disease which leads to the destruction of soft and hard tissues around osseointegrated implants. The subgingival microbiota appears to be responsible for peri-implant lesions and although the complexity of the microbiota has been reported in PI, the microbiota responsible for PI has not been identified. OBJECTIVE: The purpose of this study was to identify the microbiota in subjects who have PI, clinically healthy implants, and periodontitis-affected teeth using 16S rRNA gene clone library analysis to clarify the microbial differences...
2010: Journal of Oral Microbiology
https://www.readbyqxmd.com/read/20833884/peptostreptococcus-russellii-sp-nov-isolated-from-a-swine-manure-storage-pit
#11
Terence R Whitehead, Michael A Cotta, Enevold Falsen, Edward Moore, Paul A Lawson
Using a polyphasic approach, a taxonomic study was performed on seven strains of an unknown Gram-reaction-positive, non-spore-forming, obligately anaerobic coccus-shaped bacterium, isolated from a swine-manure storage pit. Comparative 16S rRNA gene sequencing confirmed that all seven isolates were highly related to each other and formed a hitherto unknown lineage within the clostridial rRNA XI cluster of organisms. Pairwise analysis demonstrated that the novel organism was most closely related to Peptostreptococcus anaerobius CCUG 7835(T) and Peptostreptococcus stomatis CCUG 51858(T) with 16S rRNA gene sequence similarities of 95...
August 2011: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/17714522/development-of-16s-rrna-based-probes-for-the-identification-of-gram-positive-anaerobic-cocci-isolated-from-human-clinical-specimens
#12
A C M Wildeboer-Veloo, H J M Harmsen, G W Welling, J E Degener
Fluorescent probes targeted at 16S rRNA were designed for Peptostreptococcus anaerobius and Peptostreptococcus stomatis (Pana134), Parvimonas micra (Pamic1435), Finegoldia magna (Fmag1250), Peptoniphilus asaccharolyticus (Pnasa1254), Peptoniphilus ivorii (Pnivo731), Peptoniphilus harei (Pnhar1466), Anaerococcus vaginalis (Avag1280) and Anaerococcus lactolyticus (Alac1438), based on the 16S rRNA sequences of reference strains and 88 randomly chosen clinical isolates. These strains were also used for validation of the probes...
October 2007: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/17544995/prospective-study-of-bacteremia-and-complications-with-eus-fna-of-rectal-and-perirectal-lesions
#13
Michael J Levy, Ian D Norton, Jonathan E Clain, Felicity B Enders, Ferga Gleeson, Paul J Limburg, Heidi Nelson, Elizabeth Rajan, Mark D Topazian, Kenneth K Wang, Maurits J Wiersema, Walter R Wilson
BACKGROUND & AIMS: Recent studies showed that endoscopic ultrasound (EUS)-guided fine-needle aspiration (FNA) is a low-risk procedure for causing bacteremia and infectious complications when sampling solid lesions of the upper gastrointestinal (GI) tract. As a result, antibiotics are not recommended for prophylaxis against endocarditis. Our aim was to prospectively evaluate the risk of bacteremia and other infectious complications in patients undergoing EUS FNA of lower GI tract lesions...
June 2007: Clinical Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/17403999/antimicrobial-susceptibilities-of-peptostreptococcus-anaerobius-and-the-newly-described-peptostreptococcus-stomatis-isolated-from-various-human-sources
#14
Eija Könönen, Anne Bryk, Päivi Niemi, Arja Kanervo-Nordström
Peptostreptococcus anaerobius sensu lato, currently including two closely related species, P. anaerobius and P. stomatis, is known to be more resistant than other gram-positive anaerobic cocci. We reidentified potential Peptostreptococcus isolates and tested their susceptibilities to eight antimicrobials. Notably, P. anaerobius had constantly higher values for the MIC at which 50% of the isolates are inhibited (MIC(50)) and the MIC(90) than P. stomatis.
June 2007: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/16585688/peptostreptococcus-stomatis-sp-nov-isolated-from-the-human-oral-cavity
#15
Julia Downes, William G Wade
Seven strains of anaerobic Gram-positive cocci isolated from human oral sites were subjected to a comprehensive range of phenotypic and genotypic tests. 16S rRNA gene sequence analysis revealed that the strains constituted a homogeneous group that was distinct from species with validly published names, but related to Peptostreptococcus anaerobius. All oral strains tested belonged to this group, whereas all non-oral strains studied were confirmed as P. anaerobius. A novel species, Peptostreptococcus stomatis sp...
April 2006: International Journal of Systematic and Evolutionary Microbiology
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