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p22-PHOX mRNA expression

Marta Toral, Miguel Romero, Rosario Jiménez, Iñaki Robles-Vera, Juan Tamargo, María Carmen Martínez, Francisco Pérez-Vizcaíno, Juan Duarte
Bacterial endotoxin lipopolysaccharide (LPS) activates inflammatory pathways, induces cytokine expression in the endothelium, augments reactive oxygen species (ROS) production in the vascular wall, and induces endothelial dysfunction. The aim of the present study was to analyze the effects of peroxisome proliferator-activated receptor (PPAR)β/δ activation on LPS-induced inflammation, oxidative stress and endothelial dysfunction and to determine whether uncoupling protein-2 (UCP2) plays a role in these effects...
June 15, 2016: Biochemical Pharmacology
Ning Zhang, Zheng Yang, Shi-Zhao Xiang, Ya-Ge Jin, Wen-Ying Wei, Zhou-Yan Bian, Wei Deng, Qi-Zhu Tang
Diabetic cardiomyopathy, characterized by the presence of diastolic and/or systolic myocardial dysfunction, is one of the major causes of heart failure. Nobiletin, which is extracted from the fruit peel of citrus, is reported to possess anti-inflammatory, anti-oxidative, and hypolipidemic properties. The purpose of this study was to investigate whether nobiletin exerts the therapeutic effect on streptozotocin-induced diabetic cardiomyopathy (DCM) in mice. 80 experimental male C57BL mice were randomly assigned into four groups: sham + vehicle (VEH/SH), sham + nobiletin (NOB/SH), DCM + vehicle (VEH/DM), and DCM + nobiletin (NOB/DM)...
June 2016: Molecular and Cellular Biochemistry
Wared Nour-Eldine, Crystal M Ghantous, Kazem Zibara, Leila Dib, Hawraa Issaa, Hana A Itani, Nabil El-Zein, Asad Zeidan
INTRODUCTION: Adiponectin (APN), an adipocytokine, exerts protective effects on cardiac remodeling, while angiotensin II (Ang II) induces hypertension and vascular remodeling. The potential protective role of APN on the vasculature during hypertension has not been fully elucidated yet. Here, we evaluate the molecular mechanisms of the protective role of APN in the physiological response of the vascular wall to Ang II. METHODS AND RESULTS: Rat aortic tissues were used to investigate the effect of APN on Ang II-induced vascular remodeling and hypertrophy...
2016: Frontiers in Pharmacology
Yu-Si Cheng, De-Zai Dai, Yin Dai, Dong-Dong Zhu, Bi-Cheng Liu
OBJECTIVES: Hydrogen sulphide (H2 S) has been found to be involved in cardiovascular diseases, but the exact mechanism has not been clarified. The purpose of this study was to investigate whether sodium hydrogen sulphide (NaHS), the donor of H2 S, can improve diabetic cardiomyopathy by reversing disordered calcium-handling system in sarcoplasmic reticulum (SR). METHODS: Sprague Dawley rats were injected with streptozotocin (STZ, 60 mg/kg, i.p.) to build diabetic model...
March 2016: Journal of Pharmacy and Pharmacology
Hiroyuki Ihori, Takashi Nozawa, Mitsuo Sobajima, Takuya Shida, Yasutaka Fukui, Nozomu Fujii, Hiroshi Inoue
Cardiac hypertrophy and fibrosis in heart failure with preserved ejection fraction are associated with a pro-inflammatory state and reduced NO bioavailability. Effects on myocardial structural and molecular alterations were compared between Waon therapy (WT; repeated dry sauna therapy) and statin in hypertensive rats. Seven-week-old Dahl salt-sensitive rats were assigned to 4 groups: low-salt (LS) diet, high-salt (HS) diet, HS diet with oral fluvastatin (FL; 10 mg/kg/day for 4 weeks) starting from the age of 9 weeks, and HS diet with WT treatment in a far-infrared dry sauna (39 °C for 15 min followed by 34 °C for 20 min once daily for 4 weeks)...
August 2016: Heart and Vessels
Xiaojiang Qin, Xiaomin Hou, Taigang Liang, Lijun Chen, Taotao Lu, Qingshan Li
Farrerol, a typical natural flavanone, is the major active component of Rhododendron dauricum L. The objective of this study was to evaluate the attenuation effect of farrerol against the aortic lesions in spontaneously hypertensive rats (SHR) for the first time. Twelve-week-old male SHR were orally administered with farrerol (50mg/kg/day), verapamil (50mg/kg/day, positive control), or vehicle for 8 weeks (n=10 in each group). Age-matched Wistar-Kyoto rats (WKY) served as normal controls (n=10). Our results revealed that farrerol significantly reduced the systolic blood pressure in SHR (from 177±4mmHg to 158±5mmHg) and also dramatically attenuated the aortic lesion, which is characterized by decreased media thickness, wall area, media-lumen ratio, nuclei size and an increased nuclei number (P<0...
December 15, 2015: European Journal of Pharmacology
F Rousset, F Hazane-Puch, C Pinosa, M V C Nguyen, L Grange, A Soldini, B Rubens-Duval, C Dupuy, F Morel, B Lardy
OBJECTIVES: Osteoarthritis (OA) is characterized by a progressive alteration of the biochemical properties of the articular cartilage. Inflammation plays a major role in OA, particularly through the cytokine Interleukine-1β, promoting reactive oxygen species (ROS) generation and matrix metalloproteinases (MMP) synthesis by the chondrocytes, orchestrating matrix proteolysis. NADPH oxidases (NOX) are membrane enzymes dedicated to the production of ROS. Role of oxidative stress is well established in OA; however, contribution of NOX in this process is still poorly documented...
November 2015: Osteoarthritis and Cartilage
Matthew R DiStasi, Julie A Mund, H Glenn Bohlen, Steven J Miller, David A Ingram, Michael C Dalsing, Joseph L Unthank
The present study was undertaken to establish the role of NADPH oxidase (Nox) in impaired vascular compensation to arterial occlusion that occurs in the presence of risk factors associated with oxidative stress. Diet-induced obese (DIO) mice characterized by multiple comorbidities including diabetes and hyperlipidemia were used as a preclinical model. Arterial occlusion was induced by distal femoral artery ligation in lean and DIO mice. Proximal collateral arteries were identified as the site of major (∼70%) vascular resistance to calf perfusion by distal arterial pressures, which decreased from ∼80 to ∼30 mmHg with ligation in both lean and DIO mice...
October 2015: American Journal of Physiology. Heart and Circulatory Physiology
Eric Badia, Marion Morena, Céline Lauret, Abdelhay Boulahtouf, Nathalie Boulle, Vincent Cavaillès, Patrick Balaguer, Jean Paul Cristol
BACKGROUND: Reactive oxygen species (ROS) are key players in the apoptotic effects induced by short-term tamoxifen treatment of breast cancer cells, but also in acquired resistance following long-term treatment. Whereas the use of the selective estrogen receptor down-regulator fulvestrant is promising, especially in patients who develop tamoxifen resistance, only few studies addressed its implication in the modulation of cellular redox status. METHODS: The regulation of (pro/anti)-oxidant players were first investigated at the mRNA level in a MCF-7-derived cell line after short-term (24 h) estradiol treatment...
September 2016: Breast Cancer: the Journal of the Japanese Breast Cancer Society
Yuriko Yonekura, Hideki Fujii, Kentaro Nakai, Keiji Kono, Shunsuke Goto, Masami Shinohara, Shinichi Nishi
Oxidative stress plays an important role in the pathogenesis of diabetic nephropathy. The β-blocker carvedilol has been proven to have an anti-oxidant property. The aim of the present study was to elucidate the effects of carvedilol on diabetic nephropathy. At 20 weeks of age, male Spontaneous Diabetic Torii (SDT) rats were divided into three groups based on treatment: (i) an INS group (administered insulin); (ii) a CAR group (administered 10 mg/kg per day, p.o., carvedilol); and (iii) a diabetic (DM) group (administered vehicle)...
July 14, 2015: Clinical and Experimental Pharmacology & Physiology
L E Sousa, W G Magalhães, F S Bezerra, R A S Santos, M J Campagnole-Santos, M C Isoldi, A C Alzamora
We hypothesize that exercise training (EX) reverses the level of nitric oxide (NO) and oxidative stress into rostral ventrolateral medulla (RVLM) of renovascular hypertensive rats (two kidneys, one clip - 2K1C). Microinjections of L-arginine (5 nmol), L-NAME (10 nmol), or saline (100 nl) were made into RVLM of 2K1C and normotensive (SHAM) rats sedentary (SED) or subjected to swimming for 4 weeks. mRNA expression (by qRT-PCR) of nitric oxide synthases isoforms (nNOS, eNOS, and iNOS), manganese superoxide dismutase (MnSOD), copper and zinc superoxide (Cu/ZnSOD), catalase (CAT), NADPH oxidase subunit p22(phox), concentration of thiobarbituric acid-reactive substances (TBARS), and CAT activity into RVLM were evaluated...
2015: Free Radical Research
Raji Lenin, Aravind Sankaramoorthy, Viswanathan Mohan, Muthuswamy Balasubramanyam
The mechanism of perturbed immune function in patients with T2DM is poorly understood. Recent studies imply a role for ER stress in linking immune-system alterations and metabolism. Here, we investigated whether ER stress markers and its downstream effector signals are altered in patients with type 2 diabetes along with proinflammatory augmentation. In our study, gene and protein expression of ER stress markers (GRP-78, PERK, IRE1α, ATF6, XBP-1 and CHOP) was elevated significantly (P < 0.05) in PBMCs from T2DM patients compared with control subjects...
October 2015: Journal of Leukocyte Biology
A Blum, L Ginat-Maimon, H Yehuda, N Geron, M Ben Ami, S Tamir
BACKGROUND: Weight loss surgery is the most effective treatment for morbid obesity. The mechanisms underlying the beneficial cardiovascular effects are poorly understood, although inhibition of inflammatory markers has been demonstrated. We hypothesized that anti-inflammatory and antioxidative stress reactions are responsible for the beneficial effects of bariatric surgery that have been shown in clinical trials. METHODS: The inflammatory system was studied by measuring mRNA levels of E-selectin, tumour necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and in a cell line (HUVEC-CS) of human umbilical vein endothelial cells that were incubated for 4 h with pools of serum, collected before and 3 months after surgery, from 20 women who underwent bariatric surgery for weight loss...
October 2015: Journal of Internal Medicine
Su Song, Elizabeth Kertowidjojo, Caroline Ojaimi, Beatriz Martin-Fernandez, Sharath Kandhi, Michael Wolin, Thomas H Hintze
Mild hyperhomocysteinemia (HHcy, clinically defined as less than 30 μmol/L) is an independent cardiovascular disease (CVD) risk factor, and is associated with many complications during pregnancy, such as preeclampsia (PE). The aim of this study was to assess the effect of long-term mild HHcy on cardiac metabolic function of multiparous rats. Female rats were mated 3 to 4 times and were fed with methionine in drinking water to increase plasma Hcy (2.9 ± 0.3 to 10.5 ± 2.3 μmol/L) until termination. This caused significant increase of heart weight/body weight (0...
May 2015: Physiological Reports
Li-Chin Sung, Hung-Hsing Chao, Cheng-Hsien Chen, Jen-Chen Tsai, Ju-Chi Liu, Hong-Jye Hong, Tzu-Hurng Cheng, Jin-Jer Chen
Lycopene is the most potent active antioxidant among the major carotenoids, and its use has been associated with a reduced risk for cardiovascular disease (CVD). Endothelin-1 (ET-1) is a powerful vasopressor synthesized by endothelial cells and plays a crucial role in the pathophysiology of CVD. However, the direct effects of lycopene on vascular endothelial cells have not been fully described. This study investigated the effects of lycopene on cyclic strain-induced ET-1 gene expression in human umbilical vein endothelial cells (HUVECs) and identified the signal transduction pathways that are involved in this process...
June 2015: Clinical and Experimental Pharmacology & Physiology
Kang Ding, Yan Wang, Weimin Jiang, Yu Zhang, Hongping Yin, Zhuyuan Fang
BACKGROUND: Qian Yang Yu Yin Granule (QYYYG), a traditional Chinese herbal medicine, has been indicated for renal damage in hypertension for decades in China, but little remains known regarding its underlying molecular mechanism. Therefore, we performed the current study in order to investigate the underlying molecular mechanism of QYYYG in the treatment of hypertensive renal damage. METHODS: We hypothesize that QYYYG relieves hypertensive renal injury through an angiotensin II (Ang II)-nicotinamide adenine dinucleotide phosphate (NAPDH)-oxidase (NOX)-reactive oxygen species (ROS) pathway...
2015: BMC Complementary and Alternative Medicine
Michael Edwards, Charles Czank, Gary M Woodward, Aedín Cassidy, Colin D Kay
Anthocyanins are reported to have vascular bioactivity, however their mechanisms of action are largely unknown. Evidence suggests that anthocyanins modulate endothelial function, potentially by increasing nitric oxide (NO) synthesis, or enhancing NO bioavailability. This study compared the activity of cyanidin-3-glucoside, its degradation product protocatechuic acid, and phase II metabolite, vanillic acid. Production of NO and superoxide and expression of endothelial NO synthase (eNOS), NADPH oxidase (NOX), and heme oxygenase-1 (HO-1) were established in human vascular cell models...
March 11, 2015: Journal of Agricultural and Food Chemistry
Hengjuan Guo, Jie Cao, Jinna Li, Xiaoyan Yang, Junnan Jiang, Jing Feng, Shuo Li, Jing Zhang, Baoyuan Chen
OBJECTIVE: Obstructive sleep apnea (OSA) has been implicated as a risk factor for atherosclerosis. Intermittent hypoxia (IH) induces oxidative and immuno-inflammatory alterations that could contribute to atherosclerosis. The aim of this study was to examine the effect of lymphocytes from intermittent hypoxia-exposed rats on the apoptotic signals in endothelial cells and the interventional role of tempol. METHOD: Male Wistar rats were randomly distributed into three groups (n = 8 each): control group, IH group, and tempol group (exposed to IH and treated with tempol)...
September 2015: Sleep & Breathing, Schlaf & Atmung
Guoliang Meng, Shengju Yang, Yun Chen, Wenjuan Yao, Hongyan Zhu, Wei Zhang
CONTEXT: Dihydromyricetin (DMY) displays a range of biological properties. However, whether DMY attenuates cardiomyocyte hypertrophy is unknown. OBJECTIVE: To investigate whether DMY had potential therapeutic value to protect against angiotensin II (Ang II)-induced cardiomyocyte hypertrophy. MATERIALS AND METHODS: Neonatal rat cardiomyocytes were pretreated with DMY (0-320 μM) followed with Ang II (100 nM) stimulation for 24 h, and then degree of hypertrophy was evaluated by cell surface analysis...
June 2015: Pharmaceutical Biology
Wenshuang Li, Changyuan Wang, Jinyong Peng, Jing Liang, Yue Jin, Qi Liu, Qiang Meng, Kexin Liu, Huijun Sun
In the development of atherosclerosis, naringin has exhibited potential protective effects. However, the specific mechanisms are not clearly understood. The aim of this trial was to determine the anti-oxidative and anti-inflammatory effects of naringin and uncover the mechanisms in Tumor Necrosis Factor-alpha (TNF-α) induced Human Umbilical Vein Endothelial Cells (HUVECs). Reactive Oxygen Species (ROS) were measured by flow cytometry assay. The levels of NADPH oxidase 4 (Nox4), p22(phox), intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) over-expressions were measured by qRT-PCR and Western blotting analyses...
2014: Current Pharmaceutical Biotechnology
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