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microRNA-214 inhibitor

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https://www.readbyqxmd.com/read/29113199/mir-214-targets-the-pten-mediated-pi3k-akt-signaling-pathway-and-regulates-cell-proliferation-and-apoptosis-in-ovarian-cancer
#1
Jing Liu, Weiyan Chen, Haiyan Zhang, Ting Liu, Lin Zhao
The present study aimed to investigate the potential role of microRNA (miR)-214 in targeting the phosphatase and tensin homolog (PTEN)-mediated phosphoinositide 3-kinase (PI3K)/Akt signaling pathway in ovarian cancer (OC). The target gene of miR-214 was determined by luciferase reporter gene assay and was indicated to be PTEN. Human SK-OV-3 cells were transfected with a miR-214 inhibitor and a miR-214 mimic, and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to detect relative expression of miR-214...
November 2017: Oncology Letters
https://www.readbyqxmd.com/read/28888077/microrna-214-3p-a-link-between-autophagy-and-endothelial-cell-dysfunction-in-atherosclerosis
#2
J Wang, W-N Wang, S-B Xu, H Wu, B Dai, D-D Jian, M Yang, Y-T Wu, Q Feng, J-H Zhu, L Zhang, L Zhang
AIM: Endothelial cell injury assumes a fundamental part in the pathogenesis of atherosclerosis, and endothelial cell autophagy has protective effects on the development of atherosclerosis, although the underlying molecular regulation mechanism is indistinct. This study aimed to investigate whether microRNA-214-3p (miR-214-3p) is involved in the endothelial cell autophagy regulation of atherosclerosis. METHODS: We utilized ApoE(-/-) mice provided with a high-fat diet (HFD) as atherosclerosis model...
September 9, 2017: Acta Physiologica
https://www.readbyqxmd.com/read/28772265/microrna-214-affects-fibroblast-differentiation-of-adipose-derived-mesenchymal-stem-cells-by-targeting-mitofusin-2-during-pelvic-floor-dysfunction-in-sd-rats-with-birth-trauma
#3
Jing Wu, Jiang Li, Wei-Kang Chen, Shang Liu, Jian-He Liu, Jing-Song Zhang, Ke-Wei Fang
BACKGROUND/AIMS: This study investigated whether microRNA-214 (miR-214) targets mitofusin-2 (Mfn2) in the process of fibroblast differentiation of adipose-derived mesenchymal stem cells (ADMSCs) during pelvic floor dysfunction (PFD) in Sprague Dawley (SD) rats with birth trauma. METHODS: The ADMSCs were isolated from 4-6 week male SD rats (n = 20) and were cultured and divided into the blank, miR-214 mimic negative control (NC), miR-214 mimic, miR-214 inhibitor NC, miR-214 inhibitor, empty vector, Mfn2 over-expression and miR-214 + Mfn2 over-expression groups...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28729638/inhibition-of-microrna-214-promotes-epithelial-mesenchymal-transition-process-and-induces-interstitial-cystitis-in-postmenopausal-women-by-upregulating-mfn2
#4
Jian-Wei Lv, Wei Wen, Chen Jiang, Qi-Bo Fu, Yin-Jun Gu, Ting-Ting Lv, Zhen-Dong Li, Wei Xue
Our study aims to investigate the roles that microRNA-214 (miR-214) plays in the epithelial mesenchymal transition (EMT) process and the development of interstitial cystitis (IC) in postmenopausal women by targeting Mitofusin 2 (Mfn2). IC bladder tissues and adjacent normal bladder tissues were collected from postmenopausal women. Immunohistochemistry (IHC) staining was conducted. The target relationship between miR-214 and Mfn2 was determined by a dual luciferase reporter gene assay. Adipose-derived mesenchymal stem cells (ADMSCs) were extracted from postmenopausal rats and assigned to the blank, mimics, miR-214 inhibitors, mimics negative control (NC), inhibitors NC, Mfn2 siRNA, miR-214 inhibitors and Mfn2 siRNA groups...
July 21, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28699870/microrna-mir-214-inhibits-snakehead-vesiculovirus-replication-by-targeting-the-coding-regions-of-viral-n-and-p
#5
Chi Zhang, Lizhu Yi, Shuangshuang Feng, Xueqin Liu, Jianguo Su, Li Lin, Jiagang Tu
Snakeheadvesiculovirus (SHVV), a new member of the family Rhabdoviridae, has caused enormous economic losses in snakehead fish culture during the past years in China; however, little is known about the molecular mechanisms of its pathogenicity. MicroRNAs (miRNAs) are small non-coding RNAs that play important roles in virus infection. In this study, we identified that SHVV infection downregulated miR-214 in striped snakehead (SSN-1) cells in a time- and dose-dependent manner. Notably, transfecting SSN-1 cells with miR-214 mimic significantly inhibitedSHVV replication, whereas miR-214 inhibitor promoted it, suggesting that miR-214 acted as a negative regulator of SHVV replication...
July 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28684904/dysregulation-of-microrna-214-and-pten-contributes-to-the-pathogenesis-of-hypoxic-pulmonary-hypertension
#6
HaiTao Liu, Tao Yin, Wenjun Yan, Rui Si, Bo Wang, Mai Chen, Fei Li, Qiong Wang, Ling Tao
Hypoxia-induced pulmonary hypertension, which is characterized by vascular remodeling of blood vessels, is an important complication in COPD. In this study, we found that the expression of miR-214 was differentially expressed by screening 13 candidate miRNAs in pulmonary artery smooth muscle cells (PASMCs). Additionally, using luciferase assay in PASMCs, we found that phosphatase-and-tensin homolog (PTEN) was a target of miR-214. Furthermore, the expression of PTEN was found to be substantially downregulated in PASMCs from COPD patients with pulmonary hypertension (PH) compared with normal controls by using real-time polymerase chain reaction (PCR), immunohistochemistry, and Western blot...
2017: International Journal of Chronic Obstructive Pulmonary Disease
https://www.readbyqxmd.com/read/28641614/-effect-of-microrna-214-on-migration-of-cord-blood-cd34-cells-induced-by-bone-marrow-mesenchymal-stem-cells
#7
Jing Ming, Jian Wang, Xu Ye, Hui Xu, Xing-Bing Wang
OBJECTIVE: To investigate the possible effect of MicroRNA-214 (miR-214) on migration of umbilical cord blood CD34(+) hematopoietic stem/progenitor cells induced by BM-MSC. METHODS: After transfection of the inhibitor or mimic of miR-214, the cultured supernatant of BM-MSC were collected respectively. The expression of miR-214 in BM-MSC was detected by real time quantitative PCR, the effect of supernatant on CD34(+) cell migration was evaluated by chemotaxis assays...
June 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28558818/microrna-101a-regulates-microglial-morphology-and-inflammation
#8
Reiko Saika, Hiroshi Sakuma, Daisuke Noto, Shuhei Yamaguchi, Takashi Yamamura, Sachiko Miyake
BACKGROUND: Microglia, as well as other tissue-resident macrophages, arise from yolk sac progenitors. Thus, it is likely that the central nervous system environment is critical for the acquisition of a distinct microglial phenotype. Several microRNAs that are enriched in the brain play crucial roles in brain development and may also play a role in the differentiation of microglia. METHODS: To track the differentiation of hematopoietic cells into microglia, lineage-negative bone marrow cells were co-cultured with astrocytes in the absence or presence of microRNAs or their inhibitors...
May 30, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28534937/the-inhibitory-effect-of-meg3-mir-214-aifm2-axis-on-the-growth-of-t-cell-lymphoblastic-lymphoma
#9
Fang-Yi Fan, Rui Deng, Hai Yi, Hao-Ping Sun, Yan Zeng, Guang-Cui He, Yi Su
T-cell lymphoblastic lymphoma (T-LBL) is an aggressive malignancy with poor prognosis and high recurrence rate. Long non-coding RNA (lncRNA)-MEG3 is an important tumor suppressor in various cancers. The present study investigated the potential role of maternally expressed gene 3 (MEG3) in the progression of T-LBL. Suppressed expression of MEG3 was detected in T-LBL tissues compared with adjacent histologically normal tissues. Down-regulated level of MEG3 was also found in three T-LBL cell lines (CCRF-CEM, Jurkat and SUP-T1) compared with human T-cell line H9...
May 17, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28428333/the-microrna-199a-214-cluster-targets-e-cadherin-and-claudin-2-and-promotes-high-glucose-induced-peritoneal-fibrosis
#10
Mingwen Che, Tiantian Shi, Shidong Feng, Huan Li, Xiaomin Zhang, Ning Feng, Weijuan Lou, Jianhua Dou, Guangbo Tang, Chen Huang, Guoshuang Xu, Qi Qian, Shiren Sun, Lijie He, Hanmin Wang
Serum response factor (SRF) was found to be involved in the phenotypic transition and fibrosis of the peritoneal membrane during treatment with peritoneal dialysis (PD), but the exact mechanism remains unclear. SRF regulates microRNAs (miRNAs) that contain the SRF-binding consensus (CArG) element in the promoter region. Therefore, we investigated whether the miR-199a/214 gene cluster, which contains a CArG element in its promoter, is directly regulated by SRF. High-glucose (HG) treatment significantly unregulated the expression of the miR-199a-5p/214-3p gene cluster in human peritoneal mesothelial cells (HPMCs)...
August 2017: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/28109866/microrna-214-inhibits-the-osteogenic-differentiation-of-human-osteoblasts-through-the-direct-regulation-of-baculoviral-iap-repeat-containing-7
#11
Jianmin Liu, Yan Li, Ming Luo, Zhi Yuan, Jian Liu
Osteoblasts and osteoclasts coordinate to monitor the dynamic osteogenic balance between bone formation and bone resorption. Hence, an investigation of the regulatory mechanisms underlying osteogenic osteoblast differentiation will provide more methods for bone repair and bone regeneration. In the present study, human osteoblast hFOB 1.19 cells were cultured. MicroRNA-214 (miR-214) expression significantly down-regulated during the osteogenic differentiation of hFOB 1.19 cells. In addition, miR-214 overexpression by miR-214 precursor transfection markedly inhibited the expression of alkaline phosphatase (ALP), collagen type I α1 (col1α1) and runt-related transcription factor 2 (Runx2), which concomitantly decreased ALP activity and the number of mineralized nodules but promoted the expression of signal transducer and activator of transcription 1 (STAT1), an osteogenesis blocker...
February 15, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/27959394/mir-214-suppresses-the-osteogenic-differentiation-of-bone-marrow-derived-mesenchymal-stem-cells-and-these-effects-are-mediated-through-the-inhibition-of-the-jnk-and-p38-pathways
#12
Yongzhi Guo, Lianhua Li, Jie Gao, Xiaobin Chen, Qinghua Sang
In this study, we sought to investigate the expression of microRNA (miR)-214 on the osteogenic differentiation of bone marrow‑derived mesenchymal stem cells (BMSCs) and explore the possible underlying mechanisms. We found that the overexpression of miR‑214 effectively promoted the adipocyte differentiation of BMSCs in vitro, reduced alkaline phosphatase (ALP) activity and the gene expression of collagen type I (Col I), osteocalcin (OCN) and osteopontin (OPN) in the BMSCs. We further found that the overexpression of miR‑214 suppressed the protein expression of fibroblast growth factor (FGF), phosphorylated c‑Jun N-terminal kinase (p-JNK) and phosphorylated p38 (p-p38) in the BMSCs...
January 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/27693451/crosstalk-between-the-vitamin-d-receptor-vdr-and-mir-214-in-regulating-sufu-a-hedgehog-pathway-inhibitor-in-breast-cancer-cells
#13
Fatouma Alimirah, Xinjian Peng, Akash Gupta, Liang Yuan, JoEllen Welsh, Michele Cleary, Rajendra G Mehta
The vitamin D receptor (VDR), and its ligand 1α,25-dihydroxyvitamin D3 (1,25D3) prevent breast cancer development and progression, yet the molecular mechanisms governing this are unclear. MicroRNAs (miRNAs) on the other hand, promote or inhibit breast cancer growth. To understand how VDR regulates miRNAs, we compared miRNA expression of wild-type (WT) and VDR knockout (VDRKO) breast cancer cells by a Mouse Breast Cancer miRNA PCR array. Compared to VDR WT cells, expressions of miR-214, miR-199a-3p and miR-199a-5p of the miR-199a/miR-214 cluster were 42, 15, and 10 fold higher in VDRKO cells respectively...
November 15, 2016: Experimental Cell Research
https://www.readbyqxmd.com/read/27357906/microrna-214-exerts-a-cardio-protective-effect-by-inhibition-of-fibrosis
#14
Hua Dong, Shimin Dong, Lisi Zhang, Xueping Gao, Guangwei Lv, Wei Chen, Suxia Shao
The miRNAs play important roles in regulating myocardial fibrosis. The purpose of this study was to determine the potential roles of microRNA-214 (miR-214) in cardiac fibrosis in vitro and in vivo. In vitro experiment, Ang II-induced cardiac fibroblasts (CFBs) are transfected with pre-miR-214, anti-miR-214 and their oligo controls. Gene expression was checked by Quantitative realtime-PCR (qRT-PCR) and western blotting. In the present experiment, compared with controls, expressions of collagen type I (COL I), collagen type III (COL III), transforming growth factor (TGF)-β1, and tissue inhibitors of metalloproteinase (TIMP)-1 were all increased, but matrix metalloproteinase (MMP)-1 was reduced in CFB by Ang II treatment at both mRNA and protein levels, and these alterations were found reversed by miR-214 transfection...
October 2016: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/27339596/microrna-214-promotes-peritoneal-metastasis-through-regulating-pten-negatively-in-gastric-cancer
#15
Ruijuan Xin, Feihu Bai, Yaning Feng, Mengna Jiu, Xiaogang Liu, Fangyun Bai, Yongzhan Nie, Daiming Fan
BACKGROUND AND OBJECTIVE: We aimed to investigate the effects of microRNA-214 (miR-214) on peritoneal metastasis as well as to elucidate its regulatory mechanism in gastric cancer (GC). METHODS: The expression levels of miR-214 in human GC cell lines MKN-28NM, MKN-28M, GC9811 and GC9811-P were analyzed by quantitative real-time PCR. Lentiviral miR-214, lentiviral miR-214 inhibitor, and empty lentiviral vector were transfected to GC cell lines, respectively. The roles of miR-214 in cell invasion, migration, proliferation and colony-forming ability were then analyzed...
December 2016: Clinics and Research in Hepatology and Gastroenterology
https://www.readbyqxmd.com/read/26973267/microrna-214-inhibits-left-ventricular-remodeling-in-an-acute-myocardial-infarction-rat-model-by-suppressing-cellular-apoptosis-via-the-phosphatase-and-tensin-homolog-pten
#16
Xingwei Yang, Yanjun Qin, Suxia Shao, Yueqing Yu, Chongyang Zhang, Hua Dong, Guangwei Lv, Shimin Dong
The aims of the present study were to determine the role of miR-214 on left ventricular remodeling of rat heart with acute myocardial infarction (AMI) and to further investigate the underlying mechanism of miR-214-mediated myocardial protection. AMI was induced in which adenovirus-expressing miR-214 (Ad-miR-214), anti-miR-214, or Ad-GFP had been delivered into rats hearts 4 days prior, while a phosphatase and tensin homolog (PTEN) inhibitor was administered via intra-peritoneal injection 30 minutes prior to AMI...
2016: International Heart Journal
https://www.readbyqxmd.com/read/26951965/microrna-214-acts-as-a-potential-oncogene-in-breast-cancer-by-targeting-the-pten-pi3k-akt-signaling-pathway
#17
Fang Wang, Lin Li, Zhuo Chen, Mingzhi Zhu, Yuanting Gu
Breast cancer ranks as the leading cause of cancer-related mortality in females worldwide. It has been proven that microRNAs (miRNAs or miRs), a type of non‑coding RNA, are involved in tumorigenesis. An increasing number of studies has confirmed the critical role of miR‑214 in certain types of cancer. Nevertheless, the biological function of miR‑214, as well as its underlying mechanisms of action in breast cancer remain largely unknown. In the present study, the expression of miR‑214 was found to be upregulated in four human breast cancer cell lines in contrast to its expression level in the non‑malignant breast epithelial cell line, MCF‑10A...
May 2016: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/26918939/microrna-199a-and-214-as-potential-therapeutic-targets-in-pancreatic-stellate-cells-in-pancreatic-tumor
#18
Praneeth R Kuninty, Linda Bojmar, Vegard Tjomsland, Marie Larsson, Gert Storm, Arne Östman, Per Sandström, Jai Prakash
Pancreatic stellate cells (PSCs) are the key precursor cells for cancer-associated fibroblasts (CAFs) in pancreatic tumor stroma. In this study, we explored miRNA as therapeutic targets in tumor stroma and found miR-199a-3p and miR-214-3p induced in patient-derived pancreatic CAFs and TGF-β-activated human PSCs (hPSCs). Inhibition of miR-199a/-214 using hairpin inhibitors significantly inhibited TGFβ-induced differentiation markers (e.g. α-SMA, collagen, PDGFβR), migration and proliferation. Furthermore, heterospheroids of Panc-1 and hPSCs attained smaller size with hPSCs transfected with anti-miR-199a/-214 compared to control anti-miR...
March 29, 2016: Oncotarget
https://www.readbyqxmd.com/read/26821019/identification-and-characterization-of-micrornas-in-snakehead-fish-cell-line-upon-snakehead-fish-vesiculovirus-infection
#19
Xiaodan Liu, Jiagang Tu, Junfa Yuan, Xueqin Liu, Lijuan Zhao, Farman Ullah Dawar, Muhammad Nasir Khan Khattak, Abeer M Hegazy, Nan Chen, Vikram N Vakharia, Li Lin
MicroRNAs (miRNAs) play important roles in mediating multiple biological processes in eukaryotes and are being increasingly studied to evaluate their roles associated with cellular changes following viral infection. Snakehead fish Vesiculovirus (SHVV) has caused mass mortality in snakehead fish during the past few years. To identify specific miRNAs involved in SHVV infection, we performed microRNA deep sequencing on a snakehead fish cell line (SSN-1) with or without SHVV infection. A total of 205 known miRNAs were identified when they were aligned with the known zebrafish miRNAs, and nine novel miRNAs were identified using MiRDeep2 software...
January 26, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/26507872/candidate-gene-and-microrna-expression-in-fetal-membranes-and-preterm-delivery-risk
#20
Daniel A Enquobahrie, Mark Hensley, Chunfang Qiu, Dejene F Abetew, Karin Hevner, Mahlet G Tadesse, Michelle A Williams
We investigated candidate gene and microRNA (miRNA) expression in amnion and chorion in relation to risk of preterm delivery (PTD). Amnion and chorion were separated from placenta and collected at delivery from participants who delivered at term (N = 10) and from participants who delivered preterm following spontaneous labor (sPTL-PTD; N = 10), premature rupture of membranes (PPROM-PTD; N = 10), and preeclampsia (PE-PTD; N = 10). Expression of genes (metalloproteinase [MMP] 2, MMP-9, and tissue inhibitors of MMP-1) and miRNAs (miR-199a*, -202*, -210, -214, -223, and -338) was profiled using quantitative real-time polymerase chain reaction approaches...
June 2016: Reproductive Sciences
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