Nam Hee Kim, Ngoc Bich Pham, Ronald J Quinn, Joong Sup Shim, Hee Cho, Sung Min Cho, Sung Wook Park, Jeong Hun Kim, Seung Hyeok Seok, Jong-Won Oh, Ho Jeong Kwon
R-(-)-β-O-methylsynephrine (OMe-Syn) is a naturally occurring small molecule that was identified in a previous screen as an inhibitor of angiogenesis. In this study, we conducted two animal model experiments to investigate the in vivo antiangiogenic activity of OMe-Syn. OMe-Syn significantly inhibited angiogenesis in a transgenic zebrafish model as well as in a mouse retinopathy model. To elucidate the underlying mechanisms responsible for the antiangiogenic activity of OMe-Syn, we used phage display cloning to isolate potential OMe-Syn binding proteins from human cDNA libraries and identified nucleoporin 153 kDa (NUP153) as a primary binding partner of OMe-Syn...
2015: International Journal of Biological Sciences