keyword
MENU ▼
Read by QxMD icon Read
search

Lactobacillus rhamnosus GG

keyword
https://www.readbyqxmd.com/read/28293233/the-soil-bacterium-methylococcus-capsulatus-bath-interacts-with-human-dendritic-cells-to-modulate-immune-function
#1
Stine Indrelid, Charlotte Kleiveland, René Holst, Morten Jacobsen, Tor Lea
The prevalence of inflammatory bowel disease (IBD) has increased in Western countries during the course of the twentieth century, and is evolving to be a global disease. Recently we showed that a bacterial meal of a non-commensal, non-pathogenic methanotrophic soil bacterium, Methylococcus capsulatus Bath prevents experimentally induced colitis in a murine model of IBD. The mechanism behind the effect has this far not been identified. Here, for the first time we show that M. capsulatus, a soil bacterium adheres specifically to human dendritic cells, influencing DC maturation, cytokine production, and subsequent T cell activation, proliferation and differentiation...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28287654/use-of-imaging-techniques-to-identify-efficient-controlled-release-systems-of-lactobacillus-rhamnosus-gg-during-in-vitro-digestion
#2
Justine Guerin, Jennifer Burgain, Frédéric Borges, Bhesh Bhandari, Stéphane Desobry, Joël Scher, Claire Gaiani
Matrix composition plays a crucial role in the controlled release of viable and functional bacteria in the intestine. Imaging tools such as electronic and confocal microscopies were used in this work to investigate the influence of matrix composition on matrix integrity and porosity, bacterial spatial distribution and viability during simulated in vitro digestion. L. rhamnosus GG was encapsulated in matrices having different casein/whey protein ratios. The formulation with a casein/whey ratio of 60/40 presented a porous weak gel structure that resulted in its fast disintegration in gastric media showing the presence of dead bacteria in the intestine...
March 13, 2017: Food & Function
https://www.readbyqxmd.com/read/28262688/lactobacillus-rhamnosus-gg-supernatant-enhance-neonatal-resistance-to-systemic-escherichia-coli-k1-infection-by-accelerating-development-of-intestinal-defense
#3
Xiaolong He, Qing Zeng, Santhosh Puthiyakunnon, Zhijie Zeng, Weijun Yang, Jiawen Qiu, Lei Du, Swapna Boddu, Tongwei Wu, Danxian Cai, Sheng-He Huang, Hong Cao
The objective of this study was to determine whether Lactobacillus rhamnosus GG culture supernatant (LCS) has a preventive effect against gut-derived systemic neonatal Escherichia coli (E. coli) K1 infection. The preventive effects were evaluated in human colonic carcinoma cell line Caco-2 and neonatal rat models. Our in vitro results showed that LCS could block adhesion, invasion and translocation of E. coli K1 to Caco-2 monolayer via up-regulating mucin production and maintaining intestinal integrity. In vivo experiments revealed that pre-treatment with LCS significantly decrease susceptibility of neonatal rats to oral E...
March 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28256070/selective-method-for-identification-and-quantification-of-bifidobacterium-animalis-subspecies-lactis-bb-12-bb-12-from-the-gastrointestinal-tract-of-healthy-volunteers-ingesting-a-combination-probiotic-of-bb-12-and-lactobacillus-rhamnosus-gg-lgg
#4
D D Poutsiaka, I J Mahoney, L A McDermott, L Lathrop Stern, C M Thorpe, A V Kane, C Baez-Giangreco, J McKinney, L E Davidson, R Leyva, B Goldin, D R Snydman
AIM: To develop a novel validated method for the isolation of Bifidobacterium animalis subsp lactis BB-12 (BB-12) from fecal specimens and apply it to studies of BB-12 and Lactobacillus rhamnosus GG (LGG) recovered from the healthy human gastrointestinal (GI) tract. METHODS AND RESULTS: A novel method for isolating and enumerating BB-12 was developed based on its morphologic features of growth on tetracycline-containing agar. The method identified BB-12 correctly from spiked stool close to100% of the time as validated by PCR confirmation of identity, and resulted in 97-104% recovery of BB-12...
March 3, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28256022/lactobacillus-rhamnosus-gg-probiotic-enteric-regimen-does-not-appreciably-alter-the-gut-microbiome-or-provide-protection-against-gvhd-after-allogeneic-hematopoietic-stem-cell-transplantation
#5
Elan Gorshein, Catherine Wei, Susan Ambrosy, Shanna Budney, Juliana Vivas, Angelika Shenkerman, Jacqueline Manago, Mary Kate McGrath, Anne Tyno, Yong Lin, Vimal Patel, Mecide Gharibo, Dale Schaar, Robert R Jenq, Hossein Khiabanian, Roger Strair
Graft-versus-host-disease (GVHD) is a major adverse effect associated with allogeneic stem cell transplant. Previous studies in mice indicated that administration of the probiotic Lactobacillus rhamnosus GG can reduce the incidence of GVHD after hematopoietic stem cell transplant. Here we report results from the first randomized probiotic enteric regimen trial in which allogenic hematopoietic stem cell patients were supplemented with Lactobacillus rhamnosus GG. Gut microbiome analysis confirmed a previously reported gut microbiome association with GVHD...
March 3, 2017: Clinical Transplantation
https://www.readbyqxmd.com/read/28244112/in-vitro-fermentation-of-oat-%C3%AE-glucan-and-hydrolysates-by-fecal-microbiota-and-selected-probiotic-strains
#6
Dong Ji-Lin, Yu-Xiao, Dong-Lianger, Shen Rui-Ling
BACKGROUND: Emerging evidence suggested that the prebiotic ability of β-glucan was intimately related to its molecular weight (Mw ). However, the effect of oat β-glucan with different Mw on gut homeostasis was inconsistent. Importantly, the understanding of the fermentation properties of oat β-glucan fractions was still limited. The aim of this study was to evaluate the prebiotic potential of raw and hydrolyzed oat β-glucan during in vitro fermentation by fecal microbiota and selected probiotic strains...
February 28, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28236643/selection-of-indigenous-lactobacillus-paracasei-cd4-and-lactobacillus-gastricus-btm-7-as-probiotic-assessment-of-traits-combined-with-principal-component-analysis
#7
Kavita Sharma, Rishi Mahajan, Sampan Attri, Gunjan Goel
AIMS: The population of Himalayan region is known to consume variety of fermented and non fermented foods and as a result they have been benefited in terms of overall health, because of the associated beneficial microbes. Therefore, the focus of the present study was to identify new strains of Lactic Acid Bacteria (LAB) from dairy products such as milk (cow, goat, buffalo) and fermented products (curd and buttermilk) with properties suitable for use as probiotic cultures. METHODS AND RESULTS: A total of 75 isolates tentatively identified as LAB from 100 samples were initially screened for production of β-hemolysin as indicators of virulence which resulted in 38 isolates with no haemolytic activity...
February 25, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28193434/the-effect-of-non-standard-heat-treatment-of-sheep-s-milk-on-physico-chemical-properties-sensory-characteristics-and-the-bacterial-viability-of-classical-and-probiotic-yogurt
#8
Šimun Zamberlin, Dubravka Samaržija
Classical and probiotic set yogurt were made using non-standard heat treatment of sheep's milk at 60°C/5min. Physico-chemical properties, sensory characteristics, and the viability of bacteria that originated from cultures in classical and probiotic yogurt were analysed during 21days of storage at 4°C. For the production of yogurt, a standard yogurt culture and a probiotic strain Lactobacillus rhamnosus GG were used. At the end of storage time of the classical and probiotic yogurt the totals of non-denatured whey proteins were 92...
June 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28132503/effect-of-drying-methods-on-protein-and-dna-conformation-changes-in-lactobacillus-rhamnosus-gg-cells-by-fourier-transform-infrared-spectroscopy
#9
Mya M Hlaing, Bayden R Wood, Don McNaughton, DanYang Ying, Geoff Dumsday, Mary Ann Augustin
Microencapsulation protects cells against environmental stress encountered during the production of probiotics, which are used as live microbial food ingredients. Freeze-drying and spray-drying are used in the preparation of powdered microencapsulated probiotics. This study examines the ability of Fourier transform infrared (FTIR) spectroscopy to detect differences in cells exposed to freeze-drying and spray-drying of encapsulated Lactobacillus rhamnosus GG cells. The FTIR analysis clearly demonstrated there were more significant molecular changes in lipid, fatty acid content, protein, and DNA conformation of nonencapsulated compared to encapsulated bacterial cells...
February 20, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28109094/-lactobacillus-rhamnosus-gg-conditioned-medium-prevents-e-coli-meningitis-by-inhibiting-nuclear-factor-%C3%AE%C2%BAb-pathway
#10
Qing Zeng, Xiao-Long He, Han-Sheng Xiao, Lei DU, Yu-Jing Li, Le-Cheng Chen, Hui-Wen Tian, Sheng-He Huang, Hong Cao
OBJECTIVE: To investigate whether Lactobacillus rhamnosus GG conditioned medium(LGG-CM)has preventive effect against E. coli K1-induced neuropathogenicity in vitro by inhibiting nuclear factor-κB (NF-κB) signaling pathway. METHODS: An in vitro blood-brain barrier (BBB) model was constructed using human brain microvascular endothelial cells (HBMECs). The effect of LGG-CM on E. coli-actived NF-κB signaling pathway was assayed using Western blotting. Invasion assay and polymorphonuclear leukocyte (PMN) transmigration assay were performed to explore whether LGG-CM could inhibit E...
January 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28106205/synbiotic-amazonian-palm-berry-a%C3%A3-ai-euterpe-oleracea-mart-ice-cream-improved-lactobacillus-rhamnosus-gg-survival-to-simulated-gastrointestinal-stress
#11
Mayra Garcia Maia Costa, Gabriela Namur Ooki, Antônio Diogo Silva Vieira, Raquel Bedani, Susana Marta Isay Saad
The effect of açai pulp ice cream and of its supplementation with inulin (I), whey protein concentrate (WC), and/or whey protein isolate (WI) on the viability and resistance to simulated gastrointestinal stress of the probiotic Lactobacillus (Lb.) rhamnosus GG strain throughout storage at -18 °C for up to 112 days was evaluated and morphological changes during stress were monitored. Lb. rhamnosus GG viability was stable in all formulations for up to 112 days of storage, preserving populations around 9 log CFU g(-1)...
January 20, 2017: Food & Function
https://www.readbyqxmd.com/read/28100014/mg-2-improves-the-thermotolerance-of-probiotic-lactobacillus-rhamnosus-gg-lactobacillus-casei-zhang-and-lactobacillus-plantarum-p-8
#12
Y Yang, S Huang, J Wang, G Jan, R Jeantet, X D Chen
Food-related carbohydrates and proteins are often used as thermoprotectants for probiotic lactobacilli during industrial production and processing. However, the effect of inorganic salts is rarely reported. Magnesium is the second-most abundant cation in bacteria, and commonly found in various foods. Mg(2+) homeostasis is important in Salmonella and has been reported to play a critical role in their thermotolerance. However, the role of Mg(2+) in thermotolerance of other bacteria, in particular probiotic bacteria, still remains a hypothesis...
January 18, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28067041/utility-of-thermostable-xylanases-of-mycothermus-thermophilus-in-generating-prebiotic-xylooligosaccharides
#13
Rui Ma, Yingguo Bai, Huoqing Huang, Huiying Luo, Sanfeng Chen, Yunliu Fan, Lei Cai, Bin Yao
Xylooligosaccharides as emerging prebiotics are able to promote the growth of probiotic bacteria. In the present study, four neutral, thermostable xylanases (MtXyn11A, MtXyn11At, MtXyn11B, and MtXyn11C) from compost fungus Mycothermus thermophilus CGMCC3.18119 were overexpressed in Pichia pastoris GS115 and used to produce xylooligosaccharides from beechwood xylan. The enzymes showed similar enzymatic properties (maximal activities at pH 6.0-6.5 and 65 °C) but varied in catalytic efficiency and cleaving actions...
February 3, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28008787/a-review-of-dose-responses-of-probiotics-in-human-studies
#14
A C Ouwehand
The probiotic definition requires the administration of an 'adequate amount' in order to obtain a health benefit. What that amount should be is not indicated. Here, an overview is given of studies that investigated the dose-response relation of probiotics in human interventions. Studies were divided in; meta-analyses, meta-analyses on specific probiotic strains, and studies testing two or more doses of a probiotic (combination) in the same study. Meta-analyses on the effect of probiotics on antibiotic associated diarrhoea (AAD) suggest a dose-response effect; for Clostridium difficile-associated diarrhoea on the other hand no dose-response was observed...
December 23, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/28008783/lactobacillus-rhamnosus-ncdc17-ameliorates-type-2-diabetes-by-improving-gut-function-oxidative-stress-and-inflammation-in-high-fat-diet-fed-and-streptozotocintreated-rats
#15
S Singh, R K Sharma, S Malhotra, R Pothuraju, U K Shandilya
Restoration of dysbiosed gut microbiota through probiotic may have profound effect on type 2 diabetes. In the present study, rats were fed high fat diet (HFD) for 3 weeks and injected with low dose streptozotocin to induce type 2 diabetes. Diabetic rats were then fed Lactobacillus rhamnosus NCDC 17 and L. rhamnosus GG with HFD for six weeks. L. rhamnosus NCDC 17 improved oral glucose tolerance test, biochemical parameters (fasting blood glucose, plasma insulin, glycosylated haemoglobin, free fatty acids, triglycerides, total cholesterol, low-density lipoprotein cholesterol and high-density lipoprotein cholesterol), oxidative stress (thiobarbituric acid reactive substance and activities of catalase, superoxide dismutase and glutathione peroxidase in blood and liver), bifidobacteria and lactobacilli in cecum, expression of glucagon like peptide-1 producing genes in cecum, and adiponection in epididymal fat, while decreased propionate proportions (%) in caecum, and expression of tumour necrosis factor-α and interlukin-6 in epididymal fat of diabetic rats as compared to diabetes control group...
December 23, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/28004480/a-metagenomic-study-of-the-preventive-effect-of-lactobacillus-rhamnosus-gg-on-intestinal-polyp-formation-in-apc-min-mice
#16
Y Ni, V H Y Wong, W C S Tai, J Li, W Y Wong, M M L Lee, F L Y Fong, H El-Nezami, G Panagiotou
AIMS: To investigate the in vivo effects of Lactobacillus rhamnosus GG (LGG) on intestinal polyp development and the interaction between this single-organism probiotic and the gut microbiota therein. METHODS AND RESULTS: The Apc(Min/+) mouse model was used to study the potential preventive effect of LGG on intestinal polyposis, while shotgun metagenomic sequencing was employed to characterize both taxonomic and functional changes within the gut microbial community...
December 22, 2016: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/27992668/extensively-hydrolyzed-casein-formula-alone-or-with-l-rhamnosus-gg-reduces-%C3%AE-lactoglobulin-sensitization-in-mice
#17
Rosita Aitoro, Raffaele Simeoli, Antonio Amoroso, Lorella Paparo, Rita Nocerino, Claudio Pirozzi, Margherita di Costanzo, Rosaria Meli, Carmen De Caro, Gianluca Picariello, Gianfranco Mamone, Antonio Calignano, Cathryn R Nagler, Roberto Berni Canani
BACKGROUND: Extensively hydrolyzed casein formula (EHCF) has been proposed for the prevention and is commonly used for the treatment of cow's milk allergy (CMA). The addition of the probiotic Lactobacillus rhamnosus GG (LGG) to EHCF may induce faster acquisition of tolerance to cow's milk. The mechanisms underlying this effect are largely unexplored. We investigated the effects of EHCF alone or in combination with LGG on β-lactoglobulin (BLG) sensitization in mice. METHODS: Three-week-old C3H/HeOuJ mice were sensitized by oral administration of BLG using cholera toxin as adjuvant at weekly intervals for 5 weeks (sensitization period)...
December 19, 2016: Pediatric Allergy and Immunology
https://www.readbyqxmd.com/read/27988389/probiotics-and-prevention-of-clostridium-difficile-infection
#18
E J C Goldstein, S J Johnson, P-J Maziade, C T Evans, J C Sniffen, M Millette, L V McFarland
The role of probiotics as adjunctive measures in the prevention of Clostridium difficile infection (CDI) has been controversial. However, a growing body of evidence has suggested that they have a role in primary prevention of CDI. Elements of this controversy are reviewed and the proposed mechanisms of action, the value and cost effectiveness of probiotics are addressed with a focus on three agents, Saccharomyces boulardii, Lactobacillus rhamnosus GG and the combination of Lactobacillus acidophilus CL1285, Lactobacillus casei LBC80R, Lactobacillus rhamnosus CLR2 (Bio-K+)...
December 14, 2016: Anaerobe
https://www.readbyqxmd.com/read/27975116/long-term-safety-assessment-in-children-who-received-hydrolyzed-protein-formulas-with-lactobacillus-rhamnosus-gg-a-5-year-follow-up
#19
Dmf Scalabrin, C Harris, W H Johnston, C L Berseth
Extensively hydrolyzed (EH) formula with Lactobacillus rhamnosus GG (LGG) was demonstrated to alleviate cow's milk allergy (CMA) symptoms and promote faster acquisition of tolerance to cow's milk protein. We previously demonstrated that partially hydrolyzed (PH) and EH formulas with LGG supported normal growth in healthy-term infants through 120 days of age. The objective of the current study was to evaluate growth, development, and specific adverse events through 5 years of age in participants from that cohort who continued receiving study formula...
February 2017: European Journal of Pediatrics
https://www.readbyqxmd.com/read/27939550/invited-review-microbe-mediated-aflatoxin-decontamination-of-dairy-products-and-feeds
#20
Sejeong Kim, Heeyoung Lee, Soomin Lee, Jeeyeon Lee, Jimyeong Ha, Yukyung Choi, Yohan Yoon, Kyoung-Hee Choi
Aspergillus flavus, Aspergillus parasiticus, and Aspergillus nomius contaminate corn, sorghum, rice, peanuts, tree nuts, figs, ginger, nutmeg, and milk. They produce aflatoxins, especially aflatoxin B1, which is classified as a Group 1 carcinogen by the International Agency for Research on Cancer. Many studies have focused on aflatoxin removal from food or feed, especially via microbe-mediated mechanisms-either adsorption or degradation. Of the lactic acid bacteria, Lactobacillus rhamnosus GG efficiently binds aflatoxin B1, and a peptidoglycan in the bacterium cell wall plays an important role...
February 2017: Journal of Dairy Science
keyword
keyword
119444
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"