keyword
MENU ▼
Read by QxMD icon Read
search

itaconate

keyword
https://www.readbyqxmd.com/read/28087530/pgas-a-low-ph-induced-promoter-as-a-dynamic-gene-expression-control-tool-for-the-metabolic-engineering-of-aspergillus-niger
#1
Xian Yin, Hyun-Dong Shin, Jianghua Li, Guocheng Du, Long Liu, Jian Chen
: The dynamic control of gene expression is important for adjusting fluxes in order to obtain desired products and achieve appropriate cell growth, particularly when the synthesis of a desired product drains metabolites required for cell growth. For dynamic gene expression, a promoter responsive to a particular environmental stressor is vital. Here, we report a low pH-inducible promoter, Pgas, which promotes minimal gene expression at pH values of greater than 5.0, but functioned efficiently at low pH values, such as pH 2...
January 13, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28079476/mimiviruses-and-the-human-interferon-system-viral-evasion-of-classical-antiviral-activities-but-inhibition-by-a-novel-interferon-%C3%AE-regulated-immunomodulatory-pathway
#2
Gabriel Magno de Freitas Almeida, Lorena C Ferreira Silva, Philippe Colson, Jonatas Santos Abrahao
In this review we discuss the role of mimiviruses as potential human pathogens focusing on clinical and evolutionary evidence. We also propose a novel antiviral immunomodulatory pathway controlled by interferon-β (IFN-β) and mediated by immune-responsive gene 1 (IRG1) and itaconic acid, its product. Acanthamoeba polyphaga Mimivirus (APMV) was isolated from amoebae in a hospital while investigating a pneumonia outbreak. Mimivirus ubiquity and role as protist pathogens are well understood, and its putative status as a human pathogen has been gaining strength as more evidence is being found...
January 2017: Journal of Interferon & Cytokine Research
https://www.readbyqxmd.com/read/28062355/cerebrosides-extracellular-glycolipids-secreted-by-the-selective-lignin-degrading-fungus-ceriporiopsis-subvermispora
#3
Hiroshi Nishimura, Daisuke Yamaguchi, Takashi Watanabe
Ceriporiopsis subvermispora is a selective white-rot fungus that degrades lignin at a site far from the hyphae and extracellular enzymes, without intensive damage to the cellulose. In selective ligninolysis, low molecular mass metabolites play a principal role and amphipathic substances are involved to control the degradation and transport of hydrophobic aromatic molecules, including lignin and lipids; however, secretion of the amphipathic substances by this fungus has not been well understood, except for alk(en)yl itaconates called ceriporic acids, which have a weak amphiphilicity...
January 3, 2017: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/28051098/production-of-itaconate-by-whole-cell-bioconversion-of-citrate-mediated-by-expression-of-multiple-cis-aconitate-decarboxylase-cada-genes-in-escherichia-coli
#4
Junyoung Kim, Hyung-Min Seo, Shashi Kant Bhatia, Hun-Seok Song, Jung-Ho Kim, Jong-Min Jeon, Kwon-Young Choi, Wooseong Kim, Jeong-Jun Yoon, Yun-Gon Kim, Yung-Hun Yang
Itaconate, a C5 unsaturated dicarboxylic acid, is an important chemical building block that is used in manufacturing high-value products, such as latex and superabsorbent polymers. Itaconate is produced by fermentation of sugars by the filamentous fungus Aspergillus terreus. However, fermentation by A. terreus involves a long fermentation period and the formation of various byproducts, resulting in high production costs. E. coli has been developed as an alternative for producing itaconate. However, fermentation of glucose gives low conversion yields and low productivity...
January 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/27999394/nanofiber-ion-exchange-membranes-for-the-rapid-uptake-and-recovery-of-heavy-metals-from-water
#5
Nithinart Chitpong, Scott M Husson
An evaluation of the performance of polyelectrolyte-modified nanofiber membranes was undertaken to determine their efficacy in the rapid uptake and recovery of heavy metals from impaired waters. The membranes were prepared by grafting poly(acrylic acid) (PAA) and poly(itaconic acid) (PIA) to cellulose nanofiber mats. Performance measurements quantified the dynamic ion-exchange capacity for cadmium (Cd), productivity, and recovery of Cd(II) from the membranes by regeneration. The dynamic binding capacities of Cd(II) on both types of nanofiber membrane were independent of the linear flow velocity, with a residence time of as low as 2 s...
December 20, 2016: Membranes
https://www.readbyqxmd.com/read/27979931/draft-genome-sequences-of-itaconate-producing-ustilaginaceae
#6
Elena Geiser, Florian Ludwig, Thiemo Zambanini, Nick Wierckx, Lars M Blank
Some smut fungi of the family Ustilaginaceae produce itaconate from glucose. De novo genome sequencing of nine itaconate-producing Ustilaginaceae revealed genome sizes between 19 and 25 Mbp. Comparison to the itaconate cluster of U. maydis MB215 revealed all essential genes for itaconate production contributing to metabolic engineering for improving itaconate production.
December 15, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27974733/enhanced-production-of-itaconic-acid-through-development-of-transformed-fungal-strains-of-aspergillus-terreus
#7
Woo-Shik Shin, Boonyoung Park, Dohoon Lee, Min-Kyu Oh, Gie-Taek Chun, Sangyong Kim
Metabolic engineering with a high-yielding mutant, A. terreus AN37 was performed to enhance the production of itaconic acid (IA). Reportedly, the gene cluster for IA biosynthesis is composed of four genes: reg (regulator), mtt (mitochondrial transporter), cad (cis-aconitate decarboxylase), and mfs (membrane transporter). By overexpressing each gene of the IA gene cluster in A. terreus AN37 transformed by REMI method, several transformants showing high productivity of IA were successfully obtained. One of the AN37/cad transformants could produce very high amount of IA (75 g/l) in shake-flask cultivations, showing an average of 5% higher IA titer compared to the high-yielding control strain...
December 14, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27933436/emerging-biotechnologies-for-production-of-itaconic-acid-and-its-applications-as-a-platform-chemical
#8
REVIEW
Badal C Saha
Recently, itaconic acid (IA), an unsaturated C5-dicarboxylic acid, has attracted much attention as a biobased building block chemical. It is produced industrially (>80 g L(-1)) from glucose by fermentation with Aspergillus terreus. The titer is low compared with citric acid production (>200 g L(-1)). This review summarizes the latest progress on enhancing the yield and productivity of IA production. IA biosynthesis involves the decarboxylation of the TCA cycle intermediate cis-aconitate through the action of cis-aconitate decarboxylase (CAD) enzyme encoded by the CadA gene in A...
December 8, 2016: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27873475/electrochemical-coupling-of-biomass-derived-acids-new-c8-platforms-for-renewable-polymers-and-fuels
#9
Linglin Wu, Mark Mascal, Thomas J Farmer, Sacha Pérocheau Arnaud, Maria-Angelica Wong Chang
Electrolysis of biomass-derived carbonyl compounds is an alternative to condensation chemistry for supplying products with chain length >C6 for biofuels and renewable materials production. Kolbe coupling of biomass-derived levulinic acid is used to obtain 2,7-octanedione, a new platform molecule only two low process-intensity steps removed from raw biomass. Hydrogenation to 2,7-octanediol provides a chiral secondary diol largely unknown to polymer chemistry, whereas intramolecular aldol condensation followed by hydrogenation yields branched cycloalkanes suitable for use as high-octane, cellulosic gasoline...
November 21, 2016: ChemSusChem
https://www.readbyqxmd.com/read/27847989/downstream-process-development-in-biotechnological-itaconic-acid-manufacturing
#10
REVIEW
Antonio Irineudo Magalhães, Júlio Cesar de Carvalho, Jesus David Coral Medina, Carlos Ricardo Soccol
Itaconic acid is a promising chemical that has a wide range of applications and can be obtained in large scale using fermentation processes. One of the most important uses of this biomonomer is the environmentally sustainable production of biopolymers. Separation of itaconic acid from the fermented broth has a considerable impact in the total production cost. Therefore, optimization and high efficiency downstream processes are technological challenges to make biorefineries sustainable and economically viable...
January 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27823670/a-novel-poly-deep-eutectic-solvent-based-magnetic-silica-composite-for-solid-phase-extraction-of-trypsin
#11
Kaijia Xu, Yuzhi Wang, Yixue Li, Yunxuan Lin, Haibao Zhang, Yigang Zhou
Novel poly(deep eutectic solvent) grafted silica-coated magnetic microspheres (Fe3O4@SiO2-MPS@PDES) were prepared by polymerization of choline chloride-itaconic acid (ChCl-IA) and γ-MPS-modified magnetic silica composites, and were characterized by vibrating sample magnetometer (VSM), Fourier transform infrared spectrometry (FT-IR), X-ray photoelectron spectra (XPS), thermal gravimetric analysis (TGA) and transmission electron microscope (TEM). Then the synthetic Fe3O4@SiO2-MPS@PDES microspheres were applied for the magnetic solid-phase extraction (MSPE) of trypsin for the first time...
November 23, 2016: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27793682/preparation-and-characterization-of-poly-itaconic-acid-grafted-crosslinked-chitosan-nanoadsorbent-for-high-uptake-of-hg-2-and-pb-2
#12
Huacai Ge, Tingting Hua, Jincui Wang
A novel nanoadsorbent (PIACS) with average size of 52.6nm was prepared by controlling grafted polymerization of itaconic acid on chitosan to emulsion stage and then crosslinking with glutaraldehyde. The preparation conditions were optimized and PIACS was characterized by FT-IR, XRD, SEM, TEM and TGA. The adsorption of PIACS for some metal ions was studied. The adsorption results showed that PIACS for adsorption of Hg(2+) and Pb(2+) had higher uptakes than some reported adsorbents. Various variables for adsorption of Hg(2+) and Pb(2+) were systematically researched...
February 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27792186/study-on-dicyandiamide-imprinted-polymers-with-computer-aided-design
#13
Dadong Liang, Yan Wang, Songyang Li, Yuqing Li, Miliang Zhang, Yang Li, Weishuai Tian, Junbo Liu, Shanshan Tang, Bo Li, Ruifa Jin
With the aid of theoretical calculations, a series of molecularly imprinted polymers (MIPs) were designed and prepared for the recognition of dicyandiamide (DCD) via precipitation polymerization using acetonitrile as the solvent at 333 K. On the basis of the long-range correction method of M062X/6-31G(d,p), we simulated the bonding sites, bonding situations, binding energies, imprinted molar ratios, and the mechanisms of interaction between DCD and the functional monomers. Among acrylamide (AM), N,N'-methylenebisacrylamide (MBA), itaconic acid (IA), and methacrylic acid (MAA), MAA was confirmed as the best functional monomer, because the strongest interaction (the maximum number of hydrogen bonds and the lowest binding energy) occurs between DCD and MAA, when the optimal molar ratios for DCD to the functional monomers were used, respectively...
October 26, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27776860/multi-responsive-tough-and-reversible-hydrogels-with-tunable-swelling-property
#14
Man Zhang, Rui Wang, Zhenqiang Shi, Xuelian Huang, Weifeng Zhao, Changsheng Zhao
A novel family of multi-responsive, tough, and reversible hydrogels were prepared by the combination of dipole-dipole interaction, hydrogen bonding interaction and slightly chemical cross-linking, using monomers of acrylonitrile, sodium allylsulfonate and itaconic acid. Reversible gel-sol transition was achieved by the flexible conversion of the dipole-dipole interactions between acrylonitrile-acrylonitrile and acrylonitrile-sodium thiocyanate, and the hydrogels could freely form desired shapes. The dipole-dipole and hydrogen bonding interactions improved the mechanical strength of the hydrogels with a compressive stress of 2...
October 11, 2016: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/27750034/genetic-and-biochemical-insights-into-the-itaconate-pathway-of-ustilago-maydis-enable-enhanced-production
#15
Elena Geiser, Sandra K Przybilla, Meike Engel, Wiebke Kleineberg, Linda Büttner, Eda Sarikaya, Tim den Hartog, Jürgen Klankermayer, Walter Leitner, Michael Bölker, Lars M Blank, Nick Wierckx
The Ustilaginaceae family of smut fungi, especially Ustilago maydis, gained biotechnological interest over the last years, amongst others due to its ability to naturally produce the versatile bio-based building block itaconate. Along with itaconate, U. maydis also produces 2-hydroxyparaconate. The latter was proposed to be derived from itaconate, but the underlying biochemistry and associated genes were thus far unknown. Here, we confirm that 2-hydroxyparaconate is a secondary metabolite of U. maydis and propose an extension of U...
October 14, 2016: Metabolic Engineering
https://www.readbyqxmd.com/read/27737709/genome-wide-escherichia-coli-stress-response-and-improved-tolerance-towards-industrially-relevant-chemicals
#16
Martin Holm Rau, Patricia Calero, Rebecca M Lennen, Katherine S Long, Alex T Nielsen
BACKGROUND: Economically viable biobased production of bulk chemicals and biofuels typically requires high product titers. During microbial bioconversion this often leads to product toxicity, and tolerance is therefore a critical element in the engineering of production strains. RESULTS: Here, a systems biology approach was employed to understand the chemical stress response of Escherichia coli, including a genome-wide screen for mutants with increased fitness during chemical stress...
October 13, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27725270/multifunctional-nanogels-with-dual-temperature-and-ph-responsiveness
#17
Loredana Elena Nita, Aurica P Chiriac, Alina Diaconu, Nita Tudorachi, Liliana Mititelu-Tartau
Over the last 10 years, the development of intelligent biomaterials for medical and pharmaceutical applications has attracted growing interest by combining interdisciplinary efforts. Between them nanogels represent one of the most attractive carriers for innovative drug delivery systems. In the present investigation new variants of multi-responsive nanogels have been synthesized by crosslinking poly(itaconic anhydride-co-3,9-divinyl-2,4,8,10-tetraoxaspiro [5.5] undecane) copolymer (having different molar ratios between comonomers) with 1,12-dodecandiol...
December 30, 2016: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/27704840/targeted-metabolic-profiling-of-the-tg197-mouse-model-reveals-itaconic-acid-as-a-marker-of-rheumatoid-arthritis
#18
Filippos Michopoulos, Niki Karagianni, Nichola M Whalley, Mike A Firth, Christoforos Nikolaou, Ian D Wilson, Susan E Critchlow, George Kollias, Georgios A Theodoridis
Rheumatoid arthritis is a progressive, highly debilitating disease where early diagnosis, enabling rapid clinical intervention, would provide obvious benefits to patients, healthcare systems, and society. Novel biomarkers that enable noninvasive early diagnosis of the onset and progression of the disease provide one route to achieving this goal. Here a metabolic profiling method has been applied to investigate disease development in the Tg197 arthritis mouse model. Hind limb extract profiling demonstrated clear differences in metabolic phenotypes between control (wild type) and Tg197 transgenic mice and highlighted raised concentrations of itaconic acid as a potential marker of the disease...
December 2, 2016: Journal of Proteome Research
https://www.readbyqxmd.com/read/27702509/redox-ph-dual-stimuli-responsive-degradable-salecan-g-ss-poly-ia-co-hema-hydrogel-for-release-of-doxorubicin
#19
Xinyu Hu, Yongmei Wang, Liangliang Zhang, Man Xu, Wei Dong, Jianfa Zhang
Salecan is a novel water-soluble extracellular β-glucan and possesses excellent physicochemical and biological properties. Here, redox/pH dual stimuli-responsive hydrogel based on Salecan grafted with itaconic acid (IA) and 2-hydroxyethyl methacrylate (HEMA) were prepared using disulfide-functionalized crosslinker N,N-bis(acryloyl)cystamine (BAC) for controlled drug delivery. The introduction of carboxylic groups endows the system with pH-sensitive character, swelling behavior of the hydrogel was conducted by changing the pH and Salecan content...
January 2, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27682234/butyrolactone-i-quantification-from-lovastatin-producing-aspergillus-terreus-using-tandem-mass-spectrometry-evidence-of-signalling-functions
#20
Elina K Palonen, Milla-Riina Neffling, Sheetal Raina, Annika Brandt, Tajalli Keshavarz, Jussi Meriluoto, Juhani Soini
Aspergillus terreus is an industrially important filamentous fungus producing a wide spectrum of secondary metabolites, including lovastatin and itaconic acid. It also produces butyrolactone I which has shown potential as an antitumour agent. Additionally, butyrolactone I has been implicated to have a regulating role in the secondary metabolism and morphology of A. terreus. In this study, a quantitative time-course liquid chromatography-electrospray ionisation-tandem mass spectrometry (LC-ESI-MS-MS) analysis of butyrolactone I is reported for the first time in nine-day long submerged cultures of A...
June 4, 2014: Microorganisms
keyword
keyword
119439
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"