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Laurie A Dempsey
No abstract text is available yet for this article.
May 18, 2018: Nature Immunology
Luyao Ma, Shaolong Feng, Cesar de la Fuente-Nunez, Robert E W Hancock, Xiaonan Lu
Bacterial biofilms are responsible for most clinical infections and show increased antimicrobial resistance. In this study, molecularly imprinted polymers (MIPs) were developed to specifically capture prototypical quorum sensing autoinducers [i.e., N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12AHL)], interrupt quorum sensing, and subsequently inhibit biofilm formation of Pseudomonas aeruginosa, an important human nosocomial pathogen. The synthesis of MIPs was optimized by considering the amount and type of the functional monomers itaconic acid (IA) and 2-hydroxyethyl methacrylate (HEMA)...
May 16, 2018: ACS Applied Materials & Interfaces
Giacomo Musile, Lucia Cenci, Elena Piletska, Rossella Gottardo, Alessandra M Bossi, Federica Bortolotti
The aim of the present work was to develop a novel in-house mixed-mode SPE sorbent to be used for the HPLC-Ion TrapMS determination of 16 basic drugs in urine. By using a computational modelling, a virtual monomer library was screened identifying three suitable functional monomers, methacrylic acid (MAA), itaconic acid (IA) and 2-acrylamide-2-methylpropane sulfonic acid (AMPSA), respectively. Three different sorbents were then synthetized based on these monomers, and using as cross-linker trimethylolpropane trimethacrylate (TMPTMA)...
May 9, 2018: Journal of Chromatography. A
Eric M Young, Zheng Zhao, Bianca E M Gielesen, Liang Wu, D Benjamin Gordon, Johannes A Roubos, Christopher A Voigt
Metabolic engineering requires multiple rounds of strain construction to evaluate alternative pathways and enzyme concentrations. Optimizing multigene pathways stepwise or by randomly selecting enzymes and expression levels is inefficient. Here, we apply methods from design of experiments (DOE) to guide the construction of strain libraries from which the maximum information can be extracted without sampling every possible combination. We use Saccharomyces cerevisiae as a host for a novel six-gene pathway to itaconic acid, selected by comparing alternative shunt pathways that bypass the mitochondrial TCA cycle...
May 9, 2018: Metabolic Engineering
Vittoria Infantino, Ciro Leonardo Pierri, Vito Iacobazzi
Significant metabolic changes occur in inflammation to respond to the new energetic needs of cells. Mitochondria are addressed not only to produce ATP, but also to supply substrates, such citrate, to produce pro-inflammatory molecules. In this context most of citrate is diverted from Krebs cycle and channeled into the "citrate pathway" consisting in the increase of the export of citrate into cytosol by the mitochondrial citrate carrier followed by its cleavage into acetyl-CoA and oxaloacetate by ATP citrate lyase...
May 10, 2018: Current Medicinal Chemistry
Zhongwei Yang, Hongling Wang, Yuxiao Wang, Yuhong Ren, Dong-Zhi Wei
The self-assembly of multi-enzyme into bioreactors is of extensive interest to spatially regulate valuable reactions. Despite the important progresses achieved, methods to precisely manufacture multi-enzymatic complex reactors (MECRs) are still poorly proposed both in vivo and in vitro, particularly for more than three bio-catalytically relevant enzymes. Here, we developed a sequential self-assembly system to form multitude MECRs involving three enzymes in the itaconic acid (IA) pathway with two pairs of protein-peptide interactions...
May 4, 2018: ACS Synthetic Biology
Yvonne Bordon
No abstract text is available yet for this article.
May 3, 2018: Nature Reviews. Immunology
Mieke Nieder-Heitmann, Kathleen F Haigh, Johann F Görgens
Itaconic acid has economic potential as a commodity biochemical for the sugar industry, but its production is limited due to high production costs. Using cheaper and alternative lignocellulosic feedstocks together with achieving higher product titres have been identified as potential strategies for viable IA production. Consequently the use of sugarcane bagasse and trash for the production of itaconic acid (IA) and electricity have been investigated for an integrated biorefinery, where the production facility is annexed to an existing sugar mill and new combined heat and power (CHP) plant...
April 21, 2018: Bioresource Technology
Delong Kong, Ning Qiao, Nian Wang, Zhuo Wang, Qi Wang, Zhiyong Zhou, Zhongqi Ren
A novel nanostructured magnetic ion-imprinted polymer (IIP) was synthesized for the selective adsorption of Pb(ii) from aqueous solution. The IIP was prepared on functional Fe3O4@SiO2 core/shell nanoparticles as a support. Monomer units in the polymer featured the typical bidentate ligand itaconic acid. We used ethylene glycol dimethacrylate and 2,2-azoisobisbutyronitrile as a cross-linker and an initiator, respectively. Monomers with different acid-base properties and different proportions of cross-linker were investigated to obtain high-performance adsorbents...
May 9, 2018: Physical Chemistry Chemical Physics: PCCP
C-C Chao, B O Ingram, W Lurchachaiwong, W-M Ching
Scrub typhus is an acute zoonosis caused by the obligate intracellular Gram-negative bacterium Orientia tsutsugamushi. To better understand the host response elicited by natural infection by chigger feeding, ICR mice were infected by Leptotrombidium chiangraiensis (Lc1) chiggers, and the metabolic profiles of their serum were examined over several time points after initiation of feeding. ICR mice were infected by either naive Lc1 chiggers (i.e. not infected by O. tsutsugamushi, NLc1) or O. tsutsugamushi -infected Lc1 chiggers (OLc1)...
May 2018: New Microbes and New Infections
Xin Li, Xiaoguo Ma, Renfeng Huang, Xiaowen Xie, Lihui Guo, Menyuan Zhang
Atrazine contamination of water is of considerable concern because of the potential hazard to human health. In this study, a magnetic molecularly imprinted polymer for atrazine was prepared by the surface imprinting technique using Fe3 O4 as the core, mesoporous silica as the carrier, atrazine as the template and itaconic acid as the functional monomer. The magnetic molecularly imprinted polymer was characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and vibration sample magnetometry...
April 20, 2018: Journal of Separation Science
Monika Bambouskova, Laurent Gorvel, Vicky Lampropoulou, Alexey Sergushichev, Ekaterina Loginicheva, Kendall Johnson, Daniel Korenfeld, Mary Elizabeth Mathyer, Hyeryun Kim, Li-Hao Huang, Dustin Duncan, Howard Bregman, Abdurrahman Keskin, Andrea Santeford, Rajendra S Apte, Raghav Sehgal, Britney Johnson, Gaya K Amarasinghe, Miguel P Soares, Takashi Satoh, Shizuo Akira, Tsonwin Hai, Cristina de Guzman Strong, Karine Auclair, Thomas P Roddy, Scott A Biller, Marko Jovanovic, Eynav Klechevsky, Kelly M Stewart, Gwendalyn J Randolph, Maxim N Artyomov
Metabolic regulation has been recognized as a powerful principle guiding immune responses. Inflammatory macrophages undergo extensive metabolic rewiring 1 marked by the production of substantial amounts of itaconate, which has recently been described as an immunoregulatory metabolite 2 . Itaconate and its membrane-permeable derivative dimethyl itaconate (DI) selectively inhibit a subset of cytokines 2 , including IL-6 and IL-12 but not TNF. The major effects of itaconate on cellular metabolism during macrophage activation have been attributed to the inhibition of succinate dehydrogenase2,3 , yet this inhibition alone is not sufficient to account for the pronounced immunoregulatory effects observed in the case of DI...
April 18, 2018: Nature
Grant Otto
No abstract text is available yet for this article.
April 9, 2018: Nature Reviews. Immunology
Rong Wang, Wenqing Li, Zilin Chen
Polymer monolithic column based on a green deep eutectic solvent (DES) was prepared for in-tube solid phase microextraction of non-steroidal anti-inflammatory drugs (NSAIDs) in aqueous samples. DES composed of choline chloride and itaconic acid was adopted as functional monomer to synthesize a polymeric monolith inside polydopamine-functionalized poly(ether ether ketone) (PEEK) tube. The obtained monolithic column was characterized with fourier transform infrared spectrometry, X-ray photoelectron spectrometer and field emission scanning electron microscope...
August 14, 2018: Analytica Chimica Acta
Nafiseh Shekari, Maryam Vosough, Kourosh Tabar Heidar
In the current study, gas chromatography-mass spectrometry (GC-MS) fingerprinting of herbal slimming pills assisted by chemometric methods has been presented. Deconvolution of two-way chromatographic signals of nine herbal slimming pills into pure chromatographic and spectral patterns was performed. The peak clusters were resolved using multivariate curve resolution-alternating least squares (MCR-ALS) by employing appropriate constraints. It was revealed that more useful chemical information about the composition of the slimming pills can be obtained by employing sophisticated GC-MS method coupled with proper chemometric tools yielding the extended number of identified constituents...
March 19, 2018: Forensic Science International
David Boschert, Alexandra Schneider-Chaabane, Andreas Himmelsbach, Alice Eickenscheidt, Karen Lienkamp
A series of asymmetrically disubstituted diitaconate monomers is presented. Starting from itaconic anhydride, functional groups could be placed selectively at the two non-equivalent carbonyl groups. Using 2D NMR, it was shown that the first functionalization step occurred at the carbonyl in -position to the double bond. These monomers were copolymerized with dimethylacrylamide (DMAA) to yield polymer-based synthetic mimics of antimicrobial peptides (SMAMPs). They were obtained by free radical polymerization, a metal free process, and still maintained the facial amphiphilicity at the repeat unit level...
March 30, 2018: Chemistry: a European Journal
Taher Alizadeh, Negin Hamidi, Mohamad Reza Ganjali, Faride Rafiei
Mercury ion-imprinted polymer nanoparticles (Hg-IP-NPs) were synthesized via precipitation polymerization by using itaconic acid as a functional monomer. A carbon paste electrode was impregnated with the synthesized Hg-IP-NPs and MWCNTs to obtain a highly sensitive and selective electrode for determination of Hg(II). Mercury ion is first accumulated on the electrode surface via an open circuit procedure. After reduction of Hg(II) ions to its metallic form at a negative pre-potential, square wave anodic stripping voltammetry was applied to generate the electrochemical signal...
December 5, 2017: Mikrochimica Acta
Evanna L Mills, Dylan G Ryan, Hiran A Prag, Dina Dikovskaya, Deepthi Menon, Zbigniew Zaslona, Mark P Jedrychowski, Ana S H Costa, Maureen Higgins, Emily Hams, John Szpyt, Marah C Runtsch, Martin S King, Joanna F McGouran, Roman Fischer, Benedikt M Kessler, Anne F McGettrick, Mark M Hughes, Richard G Carroll, Lee M Booty, Elena V Knatko, Paul J Meakin, Michael L J Ashford, Louise K Modis, Gino Brunori, Daniel C Sévin, Padraic G Fallon, Stuart T Caldwell, Edmund R S Kunji, Edward T Chouchani, Christian Frezza, Albena T Dinkova-Kostova, Richard C Hartley, Michael P Murphy, Luke A O'Neill
The endogenous metabolite itaconate has recently emerged as a regulator of macrophage function, but its precise mechanism of action remains poorly understood. Here we show that itaconate is required for the activation of the anti-inflammatory transcription factor Nrf2 (also known as NFE2L2) by lipopolysaccharide in mouse and human macrophages. We find that itaconate directly modifies proteins via alkylation of cysteine residues. Itaconate alkylates cysteine residues 151, 257, 288, 273 and 297 on the protein KEAP1, enabling Nrf2 to increase the expression of downstream genes with anti-oxidant and anti-inflammatory capacities...
March 28, 2018: Nature
Roberto E Flores, Ashley K Brown, Luke Taus, Julianna Khoury, Frank Glover, Kenjiro Kami, Rangaprasad Sarangarajan, Tony Walshe, Niven R Narain, Michael A Kiebish, Laura Shelton, Christos Chinopoulos, Thomas N Seyfried
Succinate is known to act as an inflammatory signal in classically activated macrophages through stabilization of HIF-1α leading to IL-1β production. Relevant to this, hypoxia is known to drive succinate accumulation and release into the extracellular milieu. The metabolic alterations associated with succinate release during inflammation and under hypoxia are poorly understood. Data are presented showing that Mycoplasma arginini infection of VM-M3 cancer cells enhances the Warburg effect associated with succinate production in mitochondria and eventual release into the extracellular milieu...
March 23, 2018: Biochimica et Biophysica Acta
Svetlana V Kamzolova, Roman V Shamin, Nadezda N Stepanova, Grigorii I Morgunov, Julia N Lunina, Ramil K Allayarov, Vladimir A Samoilenko, Igor G Morgunov
Isocitric acid exists in the form of four stereoisomers, of which only the threo -Ds-form (ICA) is a natural active compound, an intermediate of Krebs cycle, and suitable for nutritional and pharmaceutical use. In this paper, we propose a method for ICA production from ethanol by yeast Yarrowia lipolytica . The effects of temperature, pH of the medium, and aeration on the growth of the producer Y. lipolytica VKM Y-2373 and synthesis of ICA were studied. An optimal fermentation regime, which ensures a good growth of the producer and directed synthesis of the target product, was determined...
2018: BioMed Research International
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