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https://www.readbyqxmd.com/read/27784157/structural-stability-of-intelectin-1
#1
John J Kozak, Harry B Gray, Roberto A Garza-López
We study the structural stability of helical and non-helical regions in chain A of human intelectin-1. Using a geometrical model introduced previously, a computational analysis based on the recently reported crystal structure of this protein by Kiessling et al. [Nature Struct. Mole. Bio. 22 (2015), 603] is carried out to quantify the resiliency of the native state to steric perturbations. Response to these perturbations is characterized by calculating, relative to the native state, the lateral, radial and angular displacements of n-residue segments of the polypeptide chain centered on each residue...
October 26, 2016: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/27638397/characterization-of-embryo-transcriptome-of-gynogenetic-olive-flounder-paralichthys-olivaceus
#2
Zhaofei Fan, Zhihao Wu, Lijuan Wang, Yuxia Zou, Peijun Zhang, Feng You
Olive flounder Paralichthys olivaceus is an important commercially cultured marine flatfish in China, Korea, and Japan. Gynogenesis, via meiogynogenesis and mitogynogenesis, shows advantages in breeding and sex control, but the low survival rate, especially for mitogynogenesis, limits its application. In this study, we sequenced the embryo transcriptomes of gynogenetic haploid, meiogynogenetic diploid, mitogynogenetic diploid, and common diploid flounder and investigated their respective genetic characteristics by analyzing differentiated expressed genes...
September 17, 2016: Marine Biotechnology
https://www.readbyqxmd.com/read/27329687/a-zebrafish-intelectin-ortholog-agglutinates-both-gram-negative-and-gram-positive-bacteria-with-binding-capacity-to-bacterial-polysaccharide
#3
Lei Chen, Jie Yan, Weiping Sun, Yan Zhang, Chao Sui, Jing Qi, Yijun Du, Lijun Feng
Intelectins are glycan-binding lectins found in various species including cephalochordates, urochordates, fish, amphibians and mammals. But their detailed functions are not well studied in zebrafish which is a good model to study native immunity. In this study, we cloned a zebrafish intelectin ortholog, zebrafish intelectin 2 (zITLN2), which contains a conserved fibrinogen-related domain (FReD) in the N-terminus and the unique intelectin domain in the C-terminus. We examined the tissue distribution of zITLN2 in adult zebrafish and found that zITLN2 was expressed in various organs with the highest level in intestine...
August 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/26934934/soluble-human-intestinal-lactoferrin-receptor-ca-2-dependent-binding-to-sepharose-based-matrices
#4
Yuta Oshima, Kohei Seki, Masataka Shibuya, Yuki Naka, Tatsuya Yokoyama, Atsushi Sato
A soluble form of human intestinal lactoferrin receptor (shLFR) is identical to human intelectin-1 (hITLN-1), a galactofuranose-binding protein that acts as a host defense against invading pathogenic microorganisms. We found that recombinant shLFR, expressed in mammalian cells (CHO DG44, COS-1, and RK13), binds tightly to Sepharose 4 Fast Flow (FF)-based matrices in a Ca(2+)-dependent manner. This binding of shLFR to Sepharose 4 FF-based matrices was inhibited by excess D-galactose, but not by D-glucose, suggesting that shLFR recognizes repeating units of α-1,6-linked D-galactose in Sepharose 4 FF...
2016: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/26900865/chemoproteomic-profiling-of-host-and-pathogen-enzymes-active-in-cholera
#5
Stavroula K Hatzios, Sören Abel, Julianne Martell, Troy Hubbard, Jumpei Sasabe, Diana Munera, Lars Clark, Daniel A Bachovchin, Firdausi Qadri, Edward T Ryan, Brigid M Davis, Eranthie Weerapana, Matthew K Waldor
Activity-based protein profiling (ABPP) is a chemoproteomic tool for detecting active enzymes in complex biological systems. We used ABPP to identify secreted bacterial and host serine hydrolases that are active in animals infected with the cholera pathogen Vibrio cholerae. Four V. cholerae proteases were consistently active in infected rabbits, and one, VC0157 (renamed IvaP), was also active in human choleric stool. Inactivation of IvaP influenced the activity of other secreted V. cholerae and rabbit enzymes in vivo, and genetic disruption of all four proteases increased the abundance of intelectin, an intestinal lectin, and its binding to V...
April 2016: Nature Chemical Biology
https://www.readbyqxmd.com/read/26862554/coronary-patients-with-high-plasma-omentin-are-at-a-higher-cardiovascular-risk
#6
Christoph H Saely, Andreas Leiherer, Axel Muendlein, Alexander Vonbank, Philipp Rein, Kathrin Geiger, Cornelia Malin, Heinz Drexel
The adipokine omentin, also known as intelectin, is a secretory protein, expressed in visceral adipose tissue and is highly abundant in plasma. It is involved in the development of chronic inflammatory diseases, but nothing is known about its impact on the cardiovascular event risk. Here, plasma omentin was measured in 295 patients undergoing coronary angiography for the evaluation of established or suspected stable coronary artery disease (CAD). Patients were separated according to the median plasma omentin concentrations into a high and low omentin group and cardiovascular events occurring during a period of 3...
March 2016: Data in Brief
https://www.readbyqxmd.com/read/26855011/identification-and-characterization-of-a-novel-intelectin-in-the-digestive-tract-of-xenopus-laevis
#7
Saburo Nagata
The intelectin (Intl) family is a group of secretory lectins in chordates that serve multiple functions, including innate immunity, through Ca(2+)-dependent recognition of carbohydrate chains. Although six Intl family lectins have so far been reported in Xenopus laevis, none have been identified in the intestine. Using a monoclonal antibody to the Xenopus embryonic epidermal lectin (XEEL or Intl-1), I identified cross-reactive proteins in the intestines. The proteins were purified by affinity chromatography on a galactose-Sepharose column and found to be oligomers consisting of N-glycosylated 39 kDa and 40...
June 2016: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/26819645/expression-of-tmem207-in-colorectal-cancer-relation-between-tmem207-and-intelectin-1
#8
Kenichi Maeda, Chiemi Saigo, Yusuke Kito, Takuji Sakuratani, Kazuhiro Yoshida, Tamotsu Takeuchi
Recent research advances highlighted an intestinal goblet cell-produced lectin, intelectin-1 (also known as omentin-1), as a tumor suppressor. One study indicated that downregulation of intelectin-1 may be related to the unfavorable prognosis among patients with colorectal carcinoma at an advanced stage. The present study was aimed at analyzing the expression of a hitherto uncharacterized transmembrane protein TMEM207 in colorectal carcinoma, and we found that the TMEM207 function is linked to intelectin-1 processing...
2016: Journal of Cancer
https://www.readbyqxmd.com/read/26755729/structures-of-xenopus-embryonic-epidermal-lectin-reveal-a-conserved-mechanism-of-microbial-glycan-recognition
#9
Kittikhun Wangkanont, Darryl A Wesener, Jack A Vidani, Laura L Kiessling, Katrina T Forest
Intelectins (X-type lectins), broadly distributed throughout chordates, have been implicated in innate immunity. Xenopus laevis embryonic epidermal lectin (XEEL), an intelectin secreted into environmental water by the X. laevis embryo, is postulated to function as a defense against microbes. XEEL is homologous (64% identical) to human intelectin-1 (hIntL-1), which is also implicated in innate immune defense. We showed previously that hIntL-1 binds microbial glycans bearing exocyclic vicinal diol groups. It is unknown whether XEEL has the same ligand specificity...
March 11, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26619101/omentin-serum-concentration-and-hepatic-expression-in-chronic-hepatitis-c-patients-together-or-apart
#10
Michał Kukla, Marek Waluga, Brygida Adamek, Marzena Zalewska-Ziob, Janusz Kasperczyk, Aandrzej Gabriel, Rafał J Bułdak, Barbara Sobala-Szczygieł, Lucjan Kępa, Katarzyna Ziora, Krystyna Żwirska-Korczala, Edward Surma, Tomasz Sawczyn, Marek Hartleb
Chronic hepatitis C (CHC) is accompanied by numerous metabolic disorders, partially associated with altered adipokine system regulation. Omentin (intelectin-1) is a novel adipokine known to play a pivotal role in metabolic regulation in CHC. In a group of 63 CHC patients (29 men/34 women) infected with genotype 1b, aged 6.6 ± 14.6 years, serum omentin levels and its gene expression in liver tissue were examined and their association with metabolic and histopathological features was assessed. Serum omentin levels were significantly higher in CHC patients compared to controls (p < 0...
September 2015: Polish Journal of Pathology: Official Journal of the Polish Society of Pathologists
https://www.readbyqxmd.com/read/26552364/vvpe-mediates-the-intestinal-colonization-of-vibrio-vulnificus-by-the-disruption-of-tight-junctions
#11
Sei-Jung Lee, Young Hyun Jung, Jung Min Ryu, Kyung Ku Jang, Sang Ho Choi, Ho Jae Han
The disruption of gastrointestinal tight junctions and their colonization evoked by enteric pathogens are hallmarks of the pathogenesis. Vibrio (V.) vulnificus, VvpE, is an elastase which is responsible for host surface adherence and vascular permeability; however, the functional roles of VvpE in the pathogenesis of V. vulnificus (WT) are poorly understood. In the present study, we have investigated the role of VvpE in regulation of intestinal tight junctions and the colonization of WT. We found that mutation of the vvpE gene from V...
January 2016: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/26316585/the-impact-of-acute-aerobic-exercise-on-chitinase-3-like-protein-1-and-intelectin-1-expression-in-obesity
#12
Chun-Jung Huang, Aaron L Slusher, Michael Whitehurst, Marie Wells, Arun Maharaj, Yoshimi Shibata
Chitinase 3-like 1 (CHI3L1) and intelectin 1 (ITLN-1) recognize microbial N-acetylglucosamine polymer and galactofuranosyl carbohydrates, respectively. Both lectins are highly abundant in plasma and seem to play pro- and anti-inflammatory roles, respectively, in obesity and inflammatory-related illnesses. The aim of this study was to examine whether plasma levels of these lectins in obese subjects are useful for monitoring inflammatory conditions immediately influenced by acute aerobic exercise. Plasma interleukin-6, a pro-inflammatory cytokine, was also examined...
January 2016: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/26149685/autophagy-protects-against-colitis-by-the-maintenance-of-normal-gut-microflora-and-secretion-of-mucus
#13
Koichiro Tsuboi, Mayo Nishitani, Atsushi Takakura, Yasuyuki Imai, Masaaki Komatsu, Hiroto Kawashima
Genome-wide association studies of inflammatory bowel diseases identified susceptible loci containing an autophagy-related gene. However, the role of autophagy in the colon, a major affected area in inflammatory bowel diseases, is not clear. Here, we show that colonic epithelial cell-specific autophagy-related gene 7 (Atg7) conditional knock-out (cKO) mice showed exacerbation of experimental colitis with more abundant bacterial invasion into the colonic epithelium. Quantitative PCR analysis revealed that cKO mice had abnormal microflora with an increase of some genera...
August 14, 2015: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26148048/recognition-of-microbial-glycans-by-human-intelectin-1
#14
Darryl A Wesener, Kittikhun Wangkanont, Ryan McBride, Xuezheng Song, Matthew B Kraft, Heather L Hodges, Lucas C Zarling, Rebecca A Splain, David F Smith, Richard D Cummings, James C Paulson, Katrina T Forest, Laura L Kiessling
The glycans displayed on mammalian cells can differ markedly from those on microbes. Such differences could, in principle, be 'read' by carbohydrate-binding proteins, or lectins. We used glycan microarrays to show that human intelectin-1 (hIntL-1) does not bind known human glycan epitopes but does interact with multiple glycan epitopes found exclusively on microbes: β-linked D-galactofuranose (β-Galf), D-phosphoglycerol-modified glycans, heptoses, D-glycero-D-talo-oct-2-ulosonic acid (KO) and 3-deoxy-D-manno-oct-2-ulosonic acid (KDO)...
August 2015: Nature Structural & Molecular Biology
https://www.readbyqxmd.com/read/26086960/vibrio-vulnificus-vvpe-inhibits-mucin-2-expression-by-hypermethylation-via-lipid-raft-mediated-ros-signaling-in-intestinal-epithelial-cells
#15
S-J Lee, Y H Jung, S Y Oh, K K Jang, H S Lee, S H Choi, H J Han
Mucin is an important physical barrier against enteric pathogens. VvpE is an elastase encoded by Gram-negative bacterium Vibrio vulnificus; however, the functional role of VvpE in intestinal mucin (Muc) production is yet to be elucidated. The recombinant protein (r) VvpE significantly reduced the level of Muc2 in human mucus-secreting HT29-MTX cells. The repression of Muc2 induced by rVvpE was highly susceptible to the knockdown of intelectin-1b (ITLN) and sequestration of cholesterol by methyl-β-cyclodextrin...
2015: Cell Death & Disease
https://www.readbyqxmd.com/read/25965823/intelectin-1-suppresses-tumor-progression-and-is-associated-with-improved-survival-in-gastric-cancer
#16
Dan Li, Xiang Zhao, Yong Xiao, Hong Mei, Jiarui Pu, Xuan Xiang, Wanju Jiao, Huajie Song, Hongxia Qu, Kai Huang, Liduan Zheng, Qiangsong Tong
Recent evidence shows the emerging roles of intelectin 1 (ITLN1), a secretory lectin, in human cancers. Our previous studies have implicated the potential roles of ITLN1 in the aggressiveness of gastric cancer. Herein, we investigated the functions, downstream targets, and clinical significance of ITLN1 in the progression of gastric cancer. We demonstrated that ITLN1 increased the levels of hepatocyte nuclear factor 4 alpha (HNF4α), resulting in suppression of nuclear translocation and transcriptional activity of β-catenin in gastric cancer cells...
June 30, 2015: Oncotarget
https://www.readbyqxmd.com/read/25889839/intelectin-1-suppresses-the-growth-invasion-and-metastasis-of-neuroblastoma-cells-through-up-regulation-of-n-myc-downstream-regulated-gene-2
#17
Dan Li, Hong Mei, Jiarui Pu, Xuan Xiang, Xiang Zhao, Hongxia Qu, Kai Huang, Liduan Zheng, Qiangsong Tong
BACKGROUND: Recent studies have revealed the potential roles of intelectin 1 (ITLN1) in tumorigenesis. However, its functions and underlying mechanisms in neuroblastoma (NB), the most common extracranial solid tumor in childhood, still remain largely unknown. METHODS: Human neuroblastoma cell lines were treated with recombinant ITLN1 protein or stably transfected with ITLN1 expression and short hairpin RNA vectors. Gene expression and signaling pathway were detected by western blot and real-time quantitative RT-PCR...
2015: Molecular Cancer
https://www.readbyqxmd.com/read/25793526/colorectal-mucus-binds-dc-sign-and-inhibits-hiv-1-trans-infection-of-cd4-t-lymphocytes
#18
Martijn J Stax, Emily E I M Mouser, Thijs van Montfort, Rogier W Sanders, Henry J C de Vries, Henk L Dekker, Carolina Herrera, Dave Speijer, Georgios Pollakis, William A Paxton
Bodily secretions, including breast milk and semen, contain factors that modulate HIV-1 infection. Since anal intercourse caries one of the highest risks for HIV-1 transmission, our aim was to determine whether colorectal mucus (CM) also contains factors interfering with HIV-1 infection and replication. CM from a number of individuals was collected and tested for the capacity to bind DC-SIGN and inhibit HIV-1 cis- or trans-infection of CD4+ T-lymphocytes. To this end, a DC-SIGN binding ELISA, a gp140 trimer competition ELISA and HIV-1 capture/ transfer assays were utilized...
2015: PloS One
https://www.readbyqxmd.com/read/25777564/intelectin-1-suppresses-the-growth-invasion-and-metastasis-of-neuroblastoma-cells-through-up-regulation-of-n-myc-downstream-regulated-gene-2
#19
Dan Li, Hong Mei, Jiarui Pu, Xuan Xiang, Xiang Zhao, Hongxia Qu, Kai Huang, Liduan Zheng, Qiangsong Tong
BACKGROUND: Recent studies have revealed the potential roles of intelectin 1 (ITLN1) in tumorigenesis. However, its functions and underlying mechanisms in neuroblastoma (NB), the most common extracranial solid tumor in childhood, still remain largely unknown. METHODS: Human neuroblastoma cell lines were treated with recombinant ITLN1 protein or stably transfected with ITLN1 expression and short hairpin RNA vectors. Gene expression and signaling pathway were detected by western blot and real-time quantitative RT-PCR...
December 2015: Molecular Cancer
https://www.readbyqxmd.com/read/25652857/-inactivated-vaccine-for-hemorrhage-of-grass-carp-up-regulates-the-expressions-of-major-immune-related-genes-in-spleen-of-grass-carp
#20
Zhijuan Yi, Guijie Hao, Xuemei Yuan, Xiaoyi Pan, Yang Xu, Jiayun Yao, Lingyun Lin, Wenlin Yin, Jinyu Shen
OBJECTIVE: To investigate the impact of the injection of inactivated vaccine for hemorrhage of Grass carp on the expressions of main immune-related genes in spleen including immunoglobulin M (IgM), major histocompatibility complexI(MHC I), interferon I(IFN1), complement 3 (C3), interleukin-1β (IL-1β) and intelectin genes. METHODS: Ctenopharyngoden idellus kidney (CIK) cells were inoculated with Grass carp reovirus (GCRV). Then inactivated vaccine was prepared by inactivating virus suspension with formaldehyde...
February 2015: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
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