B S Bharath Kumar, Smrutirekha Mallick, H V Manjunathachar, C G Shashank, Ankur Sharma, Dudekula Nagoorvali, Simson Soren, Vyankat Gangadhar Jadhav, Sujata Pandita
Uncarboxylated osteocalcin (UcOCN), a bone derived circulating protein, has been demonstrated to influence steroidogenesis in testicular Leydig cells of murine and human species. However, the role of UcOCN in testosterone biosynthesis remains unexplored in domestic animals. The present study aimed to investigate the impact of UcOCN on the expressions of steroidogenic genes (HSD3β1, HSD3β6, CYP17A1, CYP11A1), testosterone production and GPRC6A receptor localization in buffalo Leydig cells. Leydig cells from the testes of adult Murrah buffalo were isolated, with an average cell count and viability after digestion and Percoll enrichment of 1...
February 2, 2024: Veterinary Research Communications