Igor L Medintz, Kim E Sapsford, John H Konnert, Anju Chatterji, Tianwei Lin, John E Johnson, Hedi Mattoussi
This report describes two related methods for decorating cowpea mosaic virus (CPMV) with luminescent semiconductor nanocrystals (quantum dots, QDs). Variants of CPMV are immobilized on a substrate functionalized with NeutrAvidin using modifications of biotin-avidin binding chemistry in combination with metal affinity coordination. For example, using CPMV mutants expressing available 6-histidine sequences inserted at loops on the viral coat protein, we show that these virus particles can be specifically immobilized on NeutrAvidin functionalized substrates in a controlled fashion via metal-affinity coordination...
June 7, 2005: Langmuir: the ACS Journal of Surfaces and Colloids