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Michael wang

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https://www.readbyqxmd.com/read/28811266/expression-and-purification-of-a-difficult-sarcomeric-protein-telethonin
#1
Huanbo Tan, Wencheng Su, Pengju Wang, Wenyu Zhang, Michael Sattler, Peijian Zou
Telethonin anchors the N-terminal region of titin in the Z-disk of the sarcomere by binding to two immunoglobulin-like (Ig) domains (Z1 and Z2) of titin (Z1Z2). Thereby telethonin plays an important role in myofibril assembly and in muscle development and functional regulation. The expression and purification of recombinant telethonin is very challenging. In previous studies, recombinant telethonin expressed from E. coli was refolded in the presence of Z1Z2. Here, we report various strategies to establish a reliable and efficient protocol for the preparation of telethonin and titin Z1Z2 protein...
August 12, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28810831/application-of-a-bayesian-non-linear-model-hybrid-scheme-to-sequence-data-for-genomic-prediction-and-qtl-mapping
#2
Tingting Wang, Yi-Ping Phoebe Chen, Iona M MacLeod, Jennie E Pryce, Michael E Goddard, Ben J Hayes
BACKGROUND: Using whole genome sequence data might improve genomic prediction accuracy, when compared with high-density SNP arrays, and could lead to identification of casual mutations affecting complex traits. For some traits, the most accurate genomic predictions are achieved with non-linear Bayesian methods. However, as the number of variants and the size of the reference population increase, the computational time required to implement these Bayesian methods (typically with Monte Carlo Markov Chain sampling) becomes unfeasibly long...
August 15, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28810743/layer-by-layer-molecular-assemblies-for-dye-sensitized-pho-toelectrosynthesis-cells-prepared-by-atomic-layer-deposi-tion
#3
Degao Wang, Matthew V Sheridan, Bing Shan, Byron H Farnum, Seth L Marquard, Benjamin D Sherman, Michael S Eberhart, Animesh Nayak, Christopher Dares, Atanu Das, R Morris Bullock, Thomas J Meyer
In a Dye Sensitized Photoelectrosynthesis Cell (DSPEC) the relative orientation of the catalyst and chromophore plays an important role in determining the device efficiency. Here we introduce a new, robust atomic layer deposition (ALD) procedure for the preparation of molecular chromophore-catalyst assemblies on wide bandgap semiconductors. In this procedure, solution deposited, phosphonate derivatized metal complexes on metal oxide surfaces are treated with reactive metal reagents in the gas phase by ALD to form an outer metal ion bridging group which can bind a second phosphonate containing species from solution to establish a R1-PO2-O-M-O-PO2-R2 type surface assembly...
August 15, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28810731/the-impact-of-hydrogen-bonding-on-the-dynamics-and-structure-of-protic-ionic-liquid-water-binary-mixtures
#4
Heather E Bailey, Yong-Lei Wang, Michael D Fayer
The orientational dynamics and microscopic liquid structure of a protic ionic liquid, 1-ethylimidazolium bis(trifluoromethylsulfonyl)imide (EhimNTf2), and its aprotic analogue, 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (EmimNTf2), were studied at various water concentrations using optical heterodyne-detected optical Kerr Effect (OHD-OKE) spectroscopy, linear infrared spectroscopy, and atomistic simulations. The OHD-OKE experiments essentially measure the orientational relaxation of the Ehim+ and Emim+ cations...
August 15, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28809905/endoscopic-forward-viewing-optical-coherence-tomography-and-angiography-with-mhz-swept-source
#5
Kaicheng Liang, Osman O Ahsen, Zhao Wang, Hsiang-Chieh Lee, Wenxuan Liang, Benjamin M Potsaid, Tsung-Han Tsai, Michael G Giacomelli, Vijaysekhar Jayaraman, Hiroshi Mashimo, Xingde Li, James G Fujimoto
Endoscopic optical coherence tomography (OCT) instruments are mostly side viewing and rely on at least one proximal scan, thus limiting accuracy of volumetric imaging and en face visualization. Previous forward-viewing OCT devices had limited axial scan speeds. We report a forward-viewing fiber scanning 3D-OCT probe with 900 μm field of view and 5 μm transverse resolution, imaging at 1 MHz axial scan rate in the human gastrointestinal tract. The probe is 3.3 mm diameter and 20 mm rigid length, thus enabling passage through the endoscopic channel...
August 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/28807902/h2av-facilitates-h3s10-phosphorylation-but-is-not-required-for-heat-shock-induced-chromatin-decondensation-or-transcriptional-elongation
#6
Yeran Li, Chao Wang, Weili Cai, Saheli Sengupta, Michael Zavortink, Huai Deng, Jack Girton, Jørgen Johansen, Kristen M Johansen
A model has been proposed where JIL-1 kinase-mediated H3S10 and H2Av phosphorylation is required for transcriptional elongation and heat shock-induced chromatin decondensation to occur. However, here we show that while H3S10 phosphorylation is indeed compromised in the H2Av null mutant we find that chromatin decondensation at heat shock loci is unaffected both in the absence of JIL-1 as well as of H2Av and that there is no discernable decrease in the elongating form of Pol II in either mutant. Furthermore, mRNA for the major heat shock protein Hsp70 is transcribed at robust levels in both H2Av and JIL-1 null mutants...
August 14, 2017: Development
https://www.readbyqxmd.com/read/28806450/evaluation-of-combination-measles-mumps-rubella-varicella-vaccine-introduction-in-australia
#7
Kristine Macartney, Heather F Gidding, Lieu Trinh, Han Wang, Aditi Dey, Brynley Hull, Karen Orr, Jocelynne McRae, Peter Richmond, Michael Gold, Nigel Crawford, Jennifer A Kynaston, Peter McIntyre, Nicholas Wood
Importance: Incorporating combination vaccines, such as the measles-mumps-rubella-varicella (MMRV) vaccine, into immunization schedules should be evaluated from a benefit-risk perspective. Use of MMRV vaccine poses challenges due to a recognized increased risk of febrile seizures (FSs) when used as the first dose in the second year of life. Conversely, completion by age 2 years of measles, mumps, rubella, and varicella immunization may offer improved disease control. Objective: To evaluate the effect on safety and coverage of earlier (age 18 months) scheduling of MMRV vaccine as the second dose of measles-containing vaccine (MCV) in Australia...
August 14, 2017: JAMA Pediatrics
https://www.readbyqxmd.com/read/28806074/mechanical-bond-protected-air-stable-radicals
#8
Junling Sun, Zhichang Liu, Wei-Guang Liu, Yilei Wu, Yuping Wang, Jonathan C Barnes, Keith R Hermann, William A Goddard, Michael R Wasielewski, J Fraser Stoddart
Radical templation centered around a heterotrisradical tricationic inclusion complex DB(•+)⊂DAPQT(2(•+)), assembled from an equimolar mixture of a disubstituted 4,4'-bipyridinium radical cation (DB(•+)) and an asymmetric cyclophane bisradical dication (DAPQT(2(•+))), affords a symmetric [2]catenane (SC•7PF6) and an asymmetric [2]catenane (AC•7PF6) on reaction of the 1:1 complex with diazapyrene and bipyridine, respectively. Both these highly charged [2]catenanes have been isolated as air-stable mono-radicals and characterised by EPR spectroscopy...
August 14, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28805815/aav-mediated-direct-in-vivo-crispr-screen-identifies-functional-suppressors-in-glioblastoma
#9
Ryan D Chow, Christopher D Guzman, Guangchuan Wang, Florian Schmidt, Mark W Youngblood, Lupeng Ye, Youssef Errami, Matthew B Dong, Michael A Martinez, Sensen Zhang, Paul Renauer, Kaya Bilguvar, Murat Gunel, Phillip A Sharp, Feng Zhang, Randall J Platt, Sidi Chen
A causative understanding of genetic factors that regulate glioblastoma pathogenesis is of central importance. Here we developed an adeno-associated virus-mediated, autochthonous genetic CRISPR screen in glioblastoma. Stereotaxic delivery of a virus library targeting genes commonly mutated in human cancers into the brains of conditional-Cas9 mice resulted in tumors that recapitulate human glioblastoma. Capture sequencing revealed diverse mutational profiles across tumors. The mutation frequencies in mice correlated with those in two independent patient cohorts...
August 14, 2017: Nature Neuroscience
https://www.readbyqxmd.com/read/28805812/t-bet-dependent-nkp46-innate-lymphoid-cells-regulate-the-onset-of-th17-induced-neuroinflammation
#10
Brandon Kwong, Rejane Rua, Yuanyuan Gao, John Flickinger, Yan Wang, Michael J Kruhlak, Jinfang Zhu, Eric Vivier, Dorian B McGavern, Vanja Lazarevic
The transcription factor T-bet has been associated with increased susceptibility to systemic and organ-specific autoimmunity, but the mechanism by which T-bet expression promotes neuroinflammation remains unknown. In this study, we demonstrate a cardinal role of T-bet-dependent NKp46(+) innate lymphoid cells (ILCs) in the initiation of CD4(+) TH17-mediated neuroinflammation. Loss of T-bet specifically in NKp46(+) ILCs profoundly impaired the ability of myelin-reactive TH17 cells to invade central nervous system (CNS) tissue and protected the mice from autoimmunity...
August 14, 2017: Nature Immunology
https://www.readbyqxmd.com/read/28805805/whole-tumor-section-quantitative-image-analysis-maximizes-between-pathologists-reproducibility-for-clinical-immunohistochemistry-based-biomarkers
#11
Michael Barnes, Chukka Srinivas, Isaac Bai, Judith Frederick, Wendy Liu, Anindya Sarkar, Xiuzhong Wang, Yao Nie, Bryce Portier, Monesh Kapadia, Olcay Sertel, Elizabeth Little, Bikash Sabata, Jim Ranger-Moore
Pathologists have had increasing responsibility for quantitating immunohistochemistry (IHC) biomarkers with the expectation of high between-reader reproducibility due to clinical decision-making especially for patient therapy. Digital imaging-based quantitation of IHC clinical slides offers a potential aid for improvement; however, its clinical adoption is limited potentially due to a conventional field-of-view annotation approach. In this study, we implemented a novel solely morphology-based whole tumor section annotation strategy to maximize image analysis quantitation results between readers...
August 14, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/28805803/bisphosphoglycerate-mutase-controls-serine-pathway-flux-via-3-phosphoglycerate
#12
Rob C Oslund, Xiaoyang Su, Michael Haugbro, Jung-Min Kee, Mark Esposito, Yael David, Boyuan Wang, Eva Ge, David H Perlman, Yibin Kang, Tom W Muir, Joshua D Rabinowitz
Lower glycolysis involves a series of reversible reactions, which interconvert intermediates that also feed anabolic pathways. 3-phosphoglycerate (3-PG) is an abundant lower glycolytic intermediate that feeds serine biosynthesis via the enzyme phosphoglycerate dehydrogenase, which is genomically amplified in several cancers. Phosphoglycerate mutase 1 (PGAM1) catalyzes the isomerization of 3-PG into the downstream glycolytic intermediate 2-phosphoglycerate (2-PG). PGAM1 needs to be histidine phosphorylated to become catalytically active...
August 7, 2017: Nature Chemical Biology
https://www.readbyqxmd.com/read/28805735/dietary-propolis-ameliorates-dextran-sulfate-sodium-induced-colitis-and-modulates-the-gut-microbiota-in-rats-fed-a-western-diet
#13
Kai Wang, Xiaolu Jin, Mengmeng You, Wenli Tian, Richard K Le Leu, David L Topping, Michael A Conlon, Liming Wu, Fuliang Hu
Propolis is an important hive product and considered beneficial to health. However, evidence of its potential for improving gut health is still lacking. Here we use rats to examine whether dietary supplementation with propolis could be used as a therapy for ulcerative colitis. Rats were fed with a Western style diet alone (controls) or supplemented with different amounts of Chinese propolis (0.1%, 0.2%, and 0.3%) to examine effects on acute colitis induced by 3% dextran sulphate sodium (DSS) in drinking water...
August 14, 2017: Nutrients
https://www.readbyqxmd.com/read/28804955/3m-052-as-an-adjuvant-for-a-plga-microparticle-based-leishmania-donovani-recombinant-protein-vaccine
#14
Qian Wang, Meagan A Barry, Christopher A Seid, Elissa M Hudspeth, C Patrick McAtee, Michael J Heffernan
It is believed that an effective vaccine against leishmaniasis will require a T helper type 1 (TH 1) immune response. In this study, we investigated the adjuvanticity of the Toll-like receptor (TLR) 7/8 agonist 3M-052 in combination with the Leishmania donovani 36-kDa nucleoside hydrolase recombinant protein antigen (NH36). NH36 and 3M-052 were encapsulated in separate batches of poly(lactic-co-glycolic acid) (PLGA) microparticles (MPs). The loading efficiency for NH36 was 83% and for 3M-052 was above 95%. In vitro stimulation of bone marrow-derived dendritic cells, measured by IL-12 secretion, demonstrated that 3M-052 (free or MP-formulated) had a concentration-dependent immunostimulatory effect with an optimum concentration of 2 µg/mL...
August 14, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28803854/dissociation-between-the-neural-correlates-of-recollection-and-familiarity-in-the-striatum-and-hippocampus-across-study-convergence
#15
Danielle R King, Marianne de Chastelaine, Rachael L Elward, Tracy H Wang, Michael D Rugg
In tests of recognition memory, neural activity in the striatum has consistently been reported to differ according to the study status of the test item. A full understanding of the functional significance of striatal 'retrieval success' effects is impeded by a paucity of evidence concerning whether the effects differ according to the nature of the memory signal supporting the recognition judgment (recollection vs. familiarity). Here, we address this issue through an analysis of retrieval-related striatal activity in three independent fMRI studies (total N=88)...
August 10, 2017: Behavioural Brain Research
https://www.readbyqxmd.com/read/28801450/genomic-analysis-of-hairy-cell-leukemia-identifies-novel-recurrent-genetic-alterations
#16
Benjamin H Durham, Bartlomiej Getta, Sascha Dietrich, Justin Taylor, Helen Won, James M Bogenberger, Sasinya Scott, Eunhee Kim, Young Rock Chung, Stephen S Chung, Jennifer Hüllein, Tatjana Walther, Lu Wang, Sydney X Lu, Christopher C Oakes, Raoul Tibes, Torsten Haferlach, Barry S Taylor, Martin S Tallman, Michael F Berger, Jae H Park, Thorsten Zenz, Omar Abdel-Wahab
Classical hairy cell leukemia (cHCL) is characterized by a near 100% frequency of the BRAFV600E mutation while ~30% of variant HCL (vHCL) have MAP2K1 mutations. However, recurrent genetic alterations cooperating with BRAFV600E or MAP2K1 mutations in HCL, as well as those in MAP2K1- wildtype vHCL, are not well defined. We therefore performed deep targeted mutational and copy number analysis of cHCL (n=53) and vHCL (n=8). The most common genetic alteration in cHCL outside of BRAFV600E was heterozygous loss of chromosome 7q, the minimally deleted region of which targeted wildtype BRAF, subdividing cHCL into those hemizygous versus heterozygous for the BRAFV600E mutation...
August 11, 2017: Blood
https://www.readbyqxmd.com/read/28800197/%C3%AE-functionalized-push-pull-opp-dibenzoporphyrins-as-sensitizers-for-dye-sensitized-solar-cells
#17
Hong Wang, Yi Hu, Shivaraj Yellappa, Michael B Thomas, R G Waruna Jinadasa, Alex Matus, Max Shulman, Francis D'souza
A novel class of β-functionalized push-pull zinc opp-dibenzoporphyrins were designed, synthesized and utilized as sensitizers for dye-sensitized solar cells. Spectral, electrochemical and computational studies were systematically performed to evaluate their spectral coverage, redox behavior and electronic structures. These porphyrins displayed much broader spectral coverage and more facile oxidation upon extension of π-conjugation. Free-energy calculations and femtosecond transient absorption studies (charge injection rate in the range of 1011 s-1) suggest efficient charge injection from the excited singlet state of porphyrin to the conduction band of the TiO2...
August 11, 2017: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/28800141/effects-of-strong-cyp3a-inhibition-and-induction-on-the-pharmacokinetics-of-ixazomib-an-oral-proteasome-inhibitor-results-of-drug-drug-interaction-studies-in-patients-with-advanced-solid-tumors-or-lymphoma-and-a-physiologically-based-pharmacokinetic-analysis
#18
Neeraj Gupta, Michael J Hanley, Karthik Venkatakrishnan, Alberto Bessudo, Drew W Rasco, Sunil Sharma, Bert H O'Neil, Bingxia Wang, Guohui Liu, Alice Ke, Chirag Patel, Karen Rowland Yeo, Cindy Xia, Xiaoquan Zhang, Dixie-Lee Esseltine, John Nemunaitis
At clinically relevant ixazomib concentrations, in vitro studies demonstrated that no specific cytochrome P450 (CYP) enzyme predominantly contributes to ixazomib metabolism. However, at higher than clinical concentrations, ixazomib was metabolized by multiple CYP isoforms, with the estimated relative contribution being highest for CYP3A at 42%. This multiarm phase 1 study (Clinicaltrials.gov identifier: NCT01454076) investigated the effect of the strong CYP3A inhibitors ketoconazole and clarithromycin and the strong CYP3A inducer rifampin on the pharmacokinetics of ixazomib...
August 11, 2017: Journal of Clinical Pharmacology
https://www.readbyqxmd.com/read/28799402/adsorption-of-dysprosium-on-the-graphite-0001-surface-nucleation-and-growth-at-300-k
#19
Emma J Kwolek, Huaping Lei, Ann Lii-Rosales, Mark Wallingford, Yinghui Zhou, Cai-Zhuang Wang, Michael C Tringides, James W Evans, Patricia A Thiel
We have studied nucleation and growth of Dy islands on the basal plane of graphite at 300 K using scanning tunneling microscopy, density functional theory (DFT) in a form that includes van der Waals interactions, and analytic theory. The interaction of atomic Dy with graphite is strong, while the diffusion barrier is small. Experiment shows that at 300 K, the density of nucleated islands is close to the value predicted for homogeneous nucleation, using critical nucleus size of 1 and the DFT-derived diffusion barrier...
December 7, 2016: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28799222/progression-retinal-ganglion-cell-loss-in-multiple-sclerosis-is-associated-with-new-lesions-in-the-optic-radiations
#20
Alexander Klistorner, Elizabeth C Graham, Con Yiannikas, Michael Barnett, John Parratt, Ray Garrick, Chenyu Wang, Yuyi You, Stuart L Graham
BACKGROUND: The mechanism of retinal ganglion cell (RGC) and retinal nerve fibre layer (RNFL) loss in multiple sclerosis (MS) remains unknown. This study aims to investigate the association between temporal RNFL (tRNFL) thinning and disease activity in the brain determined by T2 lesions on MRI. METHODS: 55 consecutive relapsing-remitting MS (RRMS) patients and 25 controls were enrolled. All patients underwent annual optical coherence tomography (OCT) and high-resolution MRI scans for tRNFL thickness and brain lesion volume analysis, respectively...
August 10, 2017: European Journal of Neurology: the Official Journal of the European Federation of Neurological Societies
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