keyword
MENU ▼
Read by QxMD icon Read
search

Mycobacterium complex

keyword
https://www.readbyqxmd.com/read/27913648/cd36-mediated-uptake-of-surfactant-lipids-by-human-macrophages-promotes-intracellular-growth-of-mycobacterium-tuberculosis
#1
Claire E Dodd, Charlie J Pyle, Rebecca Glowinski, Murugesan V S Rajaram, Larry S Schlesinger
Mycobacterium tuberculosis imposes a large global health burden as the airborne agent of tuberculosis. Mycobacterium tuberculosis has been flourishing in human populations for millennia and is therefore highly adapted to the lung environment. Alveolar macrophages, a major host cell niche for M. tuberculosis, are not only phagocytose inhaled microbes and particulate matter but are also crucial in catabolizing lung surfactant, a lipid-protein complex that lines the alveolar spaces. Because macrophage host defense properties can be regulated by surfactant and M...
December 15, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27913043/evaluation-of-the-genexpert-mtb-rif-assay-on-extrapulmonary-and-respiratory-samples-other-than%C3%A2-sputum-a-low-burden-country-experience
#2
Sushil Pandey, Jacob Congdon, Bradley McInnes, Alina Pop, Christopher Coulter
The aim of this study was to assess the performance of the GeneXpert MTB/RIF assay on extrapulmonary (EP) and respiratory (non-sputum) clinical samples of patients suspected of having tuberculosis (TB) from Queensland, Australia. A total of 269 EP and respiratory (non-sputum) clinical samples collected from Qld patients who were suspected of having TB were subjected to the GeneXpert MTB/RIF analysis, Ziehl-Neelsen (ZN) staining, Mycobacterium tuberculosis (MTB) culture and drug susceptibility testing. Phenotypic and genotypic data were compared...
November 29, 2016: Pathology
https://www.readbyqxmd.com/read/27911922/nucleic-acid-amplification-testing-and-sequencing-combined-with-acid-fast-staining-in-needle-biopsy-lung-tissues-for-the-diagnosis-of-smear-negative-pulmonary-tuberculosis
#3
Faming Jiang, Weiwei Huang, Ye Wang, Panwen Tian, Xuerong Chen, Zongan Liang
BACKGROUND: Smear-negative pulmonary tuberculosis (PTB) is common and difficult to diagnose. In this study, we investigated the diagnostic value of nucleic acid amplification testing and sequencing combined with acid-fast bacteria (AFB) staining of needle biopsy lung tissues for patients with suspected smear-negative PTB. METHODS: Patients with suspected smear-negative PTB who underwent percutaneous transthoracic needle biopsy between May 1, 2012, and June 30, 2015, were enrolled in this retrospective study...
2016: PloS One
https://www.readbyqxmd.com/read/27908271/host-directed-therapeutics-against-mycobacterial-infections
#4
Haruaki Tomioka, Chiaki Sano, Yutaka Tatano
The high incidence of tuberculosis (TB) in the world, especially in developing countries, the resurgence of TB in industrialized countries, and the global increase in the prevalence of Mycobacterium avium complex infections in immunocompromised hosts have prompted the quest for novel antimycobacterial drugs. However, the development of such antimicrobial chemotherapeutics is currently making very slow progress even with using the bioinformatics-based methodology for drug design. It thus appears that devising improved administration protocols for clinical treatment against intractable mycobacterial infections using existing chemotherapeutics is more practical than awaiting the development of new antimycobacterial drugs...
December 2, 2016: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/27902391/comparative-evaluation-of-the-advansure-mycobacteria-genoblot-assay-and-the-genotype-mycobacterium-cm-as-assay-for-the-identification-of-nontuberculous-mycobacteria
#5
Mina Yang, Hee Jae Huh, Hyeon Jeong Kwon, Ji-Youn Kim, Dong Joon Song, Won-Jung Koh, Chang-Seok Ki, Nam Yong Lee
In this study, to assess the performance of the AdvanSure Mycobacteria GenoBlot Assay (AdvanSure assay), we compared its performance with that of the GenoType Mycobacterium CM/AS assay (GenoType assay) for the identification of nontuberculous mycobacteria (NTM). Twenty-four reference strains and 103 consecutive clinical NTM isolates were analyzed. The accuracy rates for the 24 reference strains were 87.5% and 95.8% for the AdvanSure and GenoType assays respectively. For the 103 clinical isolates, a 91.3% (94/103) concordance rate was observed between the two assays...
October 24, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27901128/heterozygote-advantage-of-the-rs3794624-polymorphism-in-cyba-for-resistance-to-tuberculosis-in-two-chinese-populations
#6
Qianqian Liu, Shouquan Wu, Miao Xue, Andrew J Sandford, Jingcan Wu, Yu Wang, Guo Chen, Chuanmin Tao, Yin Tang, Yulin Feng, Jun Luo, Jian-Qing He
Phagocyte Nicotinamide Adenine Dinucleotide Phosphate (NADPH) oxidase complex is a key enzyme that catalyzes the production of reactive oxygen species, which mediate oxygen-dependent killing of microorganisms, such as Mycobacterium tuberculosis. P22phox, encoded by CYBA, is the key regulatory subunit of NADPH oxidase. Our study aimed to investigate the association of CYBA polymorphisms with susceptibility to tuberculosis. Three SNPs (rs9932581, rs3794624 and rs4673) were genotyped in the discovery cohort composed of Chinese Han individuals...
November 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27901051/mycobacterium-avium-mav2054-protein-induces-macrophage-apoptosis-by-targeting-mitochondria-and-reduces-intracellular-bacterial-growth
#7
Kang-In Lee, Jake Whang, Han-Gyu Choi, Yeo-Jin Son, Haet Sal Jeon, Yong Woo Back, Hye-Soo Park, Seungwha Paik, Jeong-Kyu Park, Chul Hee Choi, Hwa-Jung Kim
Mycobacterium avium complex induces macrophage apoptosis. However, the M. avium components that inhibit or trigger apoptosis and their regulating mechanisms remain unclear. We recently identified the immunodominant MAV2054 protein by fractionating M. avium culture filtrate protein by multistep chromatography; this protein showed strong immuno-reactivity in M. avium complex pulmonary disease and in patients with tuberculosis. Here, we investigated the biological effects of MAV2054 on murine macrophages. Recombinant MAV2054 induced caspase-dependent macrophage apoptosis...
November 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27894266/tuberculosis-drug-resistance-in-bamako-mali-from-2006-to-2014
#8
B Diarra, D Goita, S Tounkara, M Sanogo, B Baya, A C G Togo, M Maiga, Y S Sarro, A Kone, B Kone, O M'Baye, N Coulibaly, H Kassambara, A Cisse, M Belson, M A Polis, J Otu, F Gehre, M Antonio, S Dao, S Siddiqui, R L Murphy, B C de Jong, S Diallo
BACKGROUND: Although Drug resistance tuberculosis is not a new phenomenon, Mali remains one of the "blank" countries without systematic data. METHODS: Between 2006 and 2014, we enrolled pulmonary TB patients from local TB diagnostics centers and a university referral hospital in several observational cohort studies. These consecutive patients had first line drug susceptibility testing (DST) performed on their isolates. A subset of MDR was subsequently tested for second line drug resistance...
November 28, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27892960/new-genome-wide-algorithm-identifies-novel-in-vivo-expressed-mycobacterium-tuberculosis-antigens-inducing-human-t-cell-responses-with-classical-and-unconventional-cytokine-profiles
#9
Mariateresa Coppola, Krista E van Meijgaarden, Kees L M C Franken, Susanna Commandeur, Gregory Dolganov, Igor Kramnik, Gary K Schoolnik, Inaki Comas, Ole Lund, Corine Prins, Susan J F van den Eeden, Gro E Korsvold, Fredrik Oftung, Annemieke Geluk, Tom H M Ottenhoff
New strategies are needed to develop better tools to control TB, including identification of novel antigens for vaccination. Such Mtb antigens must be expressed during Mtb infection in the major target organ, the lung, and must be capable of eliciting human immune responses. Using genome-wide transcriptomics of Mtb infected lungs we developed data sets and methods to identify IVE-TB (in-vivo expressed Mtb) antigens expressed in the lung. Quantitative expression analysis of 2,068 Mtb genes from the predicted first operons identified the most upregulated IVE-TB genes during in-vivo pulmonary infection...
November 28, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27892856/co-infection-with-mycobacterium-bovis-does-not-alter-the-response-to-bovine-leukemia-virus-in-bola-drb3-0902-genetically-resistant-cattle
#10
Claudia M Lützelschwab, Agustina Forletti, Rosana Cepeda, Eduardo N Esteban, Omar Confalonieri, Silvina E Gutiérrez
High proviral load (HPL) profile in bovine leukemia virus infected animals poses increased risk of transmission, and development of HPL or low proviral load (LPL) profile may be attributed to host genetics. Genetic resistance and susceptibility has been mapped to the Major Histocompatibility Complex class II DRB3 gene (BoLA DRB3). The aim of this work was to determine the effect of Mycobacterium bovis infection on certain virological and host immunological parameters of BLV experimental infection. Twenty-six Argentinian Holstein calves carrying the resistance-associated marker allele BoLA DRB3*0902, susceptibility-associated marker allele BoLA DRB3*1501, or neutral BoLA DRB3 alleles, exposed to M...
September 9, 2016: Research in Veterinary Science
https://www.readbyqxmd.com/read/27891500/interspecies-communication-between-pathogens-and-immune-cells-via-bacterial-membrane-vesicles
#11
Katerina S Jurkoshek, Ying Wang, Jaffre J Athman, Marian R Barton, Pamela A Wearsch
The production of extracellular vesicles is a universal mechanism for intercellular communication that is conserved across kingdoms. Prokaryotes secrete 50-250 nm membrane vesicles (MVs) in a manner that is regulated by environmental stress and is thought to promote survival. Since many types of host-derived stress are encountered during infection, this implies an important role for MV secretion in bacterial pathogenesis. Accordingly, MVs produced by gram-positive and gram-negative pathogens contain toxins, virulence factors, and other molecules that promote survival in the host...
2016: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/27888587/tuberculosis-caused-by-mycobacterium-orygis-in-dairy-cattle-and-captured-monkeys-in-bangladesh-a-new-scenario-of-tuberculosis-in-south-asia
#12
Z Rahim, J Thapa, Y Fukushima, A G M van der Zanden, S V Gordon, Y Suzuki, C Nakajima
Mycobacterium orygis, commonly known as the oryx bacillus and a newly proposed Mycobacterium tuberculosis complex subspecies, was isolated from 18 cattle in a dairy farm and two captured rhesus monkeys in a zoo in Bangladesh. All the infected animals had tuberculosis lesions in their lungs, suggesting transmission and infection with M. orygis by an airborne route. The 20 isolates were analysed using a range of conventional and molecular typing methods, and RD-deletion typing and sequencing of selected genes confirmed the isolates as M...
November 25, 2016: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/27888200/effect-of-principal-component-analysis-centering-and-scaling-on-classification-of-mycobacteria-from-raman-spectra
#13
Cynthia Hanson, Michael Sieverts, Elizabeth Vargis
Raman spectroscopy has been used for decades to detect and identify biological substances as it provides specific molecular information. Spectra collected from biological samples are often complex, requiring the aid of data truncation techniques such as principal component analysis (PCA) and multivariate classification methods. Classification results depend on the proper selection of principal components (PCs) and how PCA is performed (scaling and/or centering). There are also guidelines for choosing the optimal number of PCs such as a scree plot, Kaiser criterion, or cumulative percent variance...
November 25, 2016: Applied Spectroscopy
https://www.readbyqxmd.com/read/27882275/a-case-of-an-enigmatic-pulmonary-infiltrate
#14
Uroosa Ibrahim, Amina Saqib, Michel Chalhoub, Jean Paul Atallah
The differential diagnosis of a pulmonary mass in an immunosuppressed host with a history of cancer is broad and includes malignant, infectious and inflammatory etiologies. Mycobacterium avium complex (MAC) is a rare cause of opportunistic infection in susceptible individuals that can present as either localized or disseminated disease. On radiologic studies, the pulmonary disease can manifest as heterogeneous linear or nodular densities, a mass-like lesion, or thin-walled cavitary lesions. We present the case of pulmonary MAC in a patient with a history of lung cancer requiring lobectomy, and splenic lymphoma being treated with chemotherapy, presenting with extreme fatigue and a fludeoxyglucose (FDG)-avid mass on positron emission tomography-computed tomography (PET-CT)...
October 13, 2016: Curēus
https://www.readbyqxmd.com/read/27876856/the-trend-and-the-disease-prediction-of-vascular-endothelial-growth-factor-and-placenta-growth-factor-in-nontuberculous-mycobacterial-lung-disease
#15
Chou-Han Lin, Chin-Chung Shu, Chia-Lin Hsu, Shih-Lung Cheng, Jann-Yuan Wang, Chong-Jen Yu, Li-Na Lee
Nontuberculous mycobacteria (NTM)-lung disease (LD) is an increasing health problem worldwide. The diagnosis of this disease remains difficult, however the application of placenta growth factor (PlGF) and vascular endothelial growth factor (VEGF) has not yet been studied. We screened patients with Mycobacterium avium complex or M. abscessus isolated from sputum, and enrolled 32 patients with NTM-LD and 93 with NTM pulmonary colonization. The NTM-LD group had a lower body mass index, higher proportion of bronchiectasis, more respiratory symptoms and pulmonary lesions, and higher titers of sputum acid-fast stain than the NTM pulmonary colonization group...
November 23, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27876506/fungal-peptides-from-pneumonitis-hypersensitivity-etiologic-agents-are-able-to-induce-specific-cellular-immune-response
#16
Anne-Pauline Bellanger, Thibaud Lignon, Yann Godet, Bénédicte Rognon, Gabriel Reboux, Houssein Gbaguidi-Haore, Christophe Borg, Laurence Millon
PURPOSE: Hypersensitivity pneumonitis (HP) is an immunoallergic disease due to chronic exposure to high quantities of different microorganisms such as Mycobacterium immunogenum (Mi), a mycobacterium, and Lichtheimia corymbifera (Lc), a filamentous fungus. It has recently been demonstrated that the protein DLDH (dihydrolipoyl dehydrogenase), is common to these microorganisms. This study aimed to investigate the immune potential of overlapping peptide pools covering the MiDLDH and LcDLDH...
November 19, 2016: Journal of Immunological Methods
https://www.readbyqxmd.com/read/27874159/evaluation-of-genexpert-mtb-rif-for-detection-of-mycobacterium-tuberculosis-complex-and-rpo-b-gene-in-respiratory-and-non-respiratory-clinical-specimens-at-a-tertiary-care-teaching-hospital-in-saudi-arabia
#17
Ali M Somily, Mazin A Barry, Hanan A Habib, Fawzia E Alotaibi, Fahad A Al-Zamil, Mohammed A Khan, Mohammed S Sarwar, Nawab D Bakhash, Abdulkarim A Alrabiaah, Zahid A Shakoor, Abiola C Senok
To assess the performance of Xpert MTB/RIF, an automated molecular test for Mycobacterium tuberculosis (MTB) and resistance to rifampin (RIF), against smear microscopy and culture method for diagnosis of MTB infection. Methods: This is a retrospective analysis of 103 respiratory and 137 non-respiratory patient specimens suspected of tuberculosis at King Khalid University Hospital, Riyadh, Kingdom of Saudi Arabia performed between April 2014 and March 2015. Each sample underwent smear microscopy, mycobacterial culture, and GeneXpert MTB/RIF test...
December 2016: Saudi Medical Journal
https://www.readbyqxmd.com/read/27872068/missense-mutations-in-the-unfoldase-clpc1-of-the-caseinolytic-protease-complex-are-associated-with-pyrazinamide-resistance-in-mycobacterium-tuberculosis
#18
Michelle Yee, Pooja Gopal, Thomas Dick
Previously we showed that mutations in Mycobacterium tuberculosis panD, involved in coenzyme A biosynthesis, cause resistance against pyrazinoic acid, the bioactive component of the prodrug pyrazinamide. To determine whether additional resistance mechanisms can be identified, we isolated mutants resistant against pyrazinoic acid and subjected panD wildtype strains to whole genome sequencing. 8 out of 9 resistant strains harbored missense mutations in the unfoldase ClpC1 associated with the caseinolytic protease complex...
November 21, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27872066/clinical-characteristics-and-treatment-outcomes-of-patients-with-macrolide-resistant-mycobacterium-massiliense-lung-disease
#19
Hayoung Choi, Su-Young Kim, Hyun Lee, Byung Woo Jhun, Hye Yun Park, Kyeongman Jeon, Dae Hun Kim, Hee Jae Huh, Chang-Seok Ki, Nam Yong Lee, Seung-Heon Lee, Sung Jae Shin, Charles L Daley, Won-Jung Koh
Macrolide antibiotics are cornerstones in the treatment of Mycobacterium massiliense lung disease. Despite the emergence of resistance, limited data are available on macrolide-resistant M massiliense lung disease. This study evaluated the clinical features and treatment outcomes of patients and the molecular characteristics of macrolide-resistant M massiliense isolates. We performed a retrospective review of medical records and genetic analysis of clinical isolates from 15 patients with macrolide-resistant M massiliense lung disease between September 2005 and February 2015...
November 21, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27872065/in-vitro-susceptibility-testing-of-bedaquiline-against-mycobacterium-avium-complex
#20
Barbara A Brown-Elliott, Julie V Philley, David E Griffith, Foram Thakkar, Richard J Wallace
We performed bedaquiline broth microdilution susceptibilities using Clinical Laboratory Standards Institute (CLSI) guidelines on 103 respiratory isolates of Mycobacterium avium complex (MAC), including multidrug resistant isolates. Approximately 90% of isolates had bedaquiline minimal inhibitory concentrations (MICs) ≤0.008 μg/mL and 102/103 isolates had MICs ≤0.015 μg/mL. Bedaquiline has excellent potential for use in patients with MAC, although for reasons of its metabolism by the cytochrome P450 system, it should not be given with rifampin...
November 21, 2016: Antimicrobial Agents and Chemotherapy
keyword
keyword
119369
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"