Tao Wu, Chenlan Shen, Zhenzhen Zhao, Mengyuan Lyu, Hao Bai, Xuejiao Hu, Junwei Zhao, Ru Zhang, Kun Qian, Gaolian Xu, Binwu Ying
Efficient diagnosis of mycobacterial infections can effectively manage and prevent the transmission of infectious diseases. Unfortunately, existing diagnostic strategies are challenged by long assay times, high costs, and highly specialized expertise to distinguish between pulmonary tuberculosis (PTB) and nontuberculous mycobacterial pulmonary diseases (NTM-PDs). Herein, in this study, an optimized 3D paper-based analytical device (µPAD) is incorporated with a closed lateral flow (LF) strip into a loop-mediated isothermal amplification (LAMP) device (3D-µPAD-LF-LAMP) for rapid, low-cost, and visual detection of pathogenic mycobacteria...
March 11, 2024: Small Methods