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https://www.readbyqxmd.com/read/28940727/whole-genome-sequencing-and-antigenic-analysis-of-the-first-equine-influenza-virus-identified-in-turkey
#1
Jacinta Gahan, Marie Garvey, Sarah Gildea, Gildea Gür, Anil Kagankaya, Ann Cullinane
BACKGROUND: In 2013 there was an outbreak of acute respiratory disease in racehorses in Turkey. The clinical signs were consistent with equine influenza (EI). OBJECTIVE: The aim was to confirm the cause of the outbreak and characterise the causal virus. METHODS: A pan-reactive influenza type A real time RT-PCR and a rapid antigen detection kit were used for confirmatory diagnosis of equine influenza virus (EIV). Immunological susceptibility to EIV was examined using Single Radial Haemolysis and ELISA...
September 21, 2017: Influenza and Other Respiratory Viruses
https://www.readbyqxmd.com/read/28928478/biomimetically-engineered-amphotericin-b-nano-aggregates-circumvent-toxicity-constraints-and-treat-systemic-fungal-infection-in-experimental-animals
#2
Qamar Zia, Owais Mohammad, Mohd Ahmar Rauf, Wasi Khan, Swaleha Zubair
Biomimetic synthesis of nanoparticles offers a convenient and bio friendly approach to fabricate complex structures with sub-nanometer precision from simple precursor components. In the present study, we have synthesized nanoparticles of Amphotericin B (AmB), a potent antifungal agent, using Aloe vera leaf extract. The synthesis of AmB nano-assemblies (AmB-NAs) was established employing spectro-photometric and electron microscopic studies, while their crystalline nature was established by X-ray diffraction...
September 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28924148/investigation-of-the-inhibition-effect-and-mechanism-of-myricetin-to-suilysin-by-molecular-modeling
#3
Xiaodi Niu, Lin Sun, Guizhen Wang, Yawen Gao, Yanan Yang, Xiyan Wang, Hongsu Wang
In the present study, the inhibitory effect and mechanism of myricetin, a natural flavonoid compound, in relation to Suilysin (SLY) were investigated through molecular dynamics simulations, mutational analysis and fluorescence-quenching assays. Myricetin is a potential inhibitor that does not exhibit antimicrobial activity but has been shown to inhibit SLY cytotoxicity. Molecular dynamics simulations and mutational analysis revealed that myricetin binds directly to SLY in the gap between domains 2 and 3, an important region for oligomerization and pore formation...
September 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28919009/distinct-patterns-of-response-to-transfusion-therapy-for-different-chronic-complications-of-sickle-cell-disease-a-useful-insight
#4
Dimitris A Tsitsikas, Funmilayo Orebayo, Alexandra Agapidou, Roger J Amos
Two main sub-phenotypes have been described in sickle cell disease: one with higher baseline haemoglobin and a higher rate of painful crises and one with lower baseline haemoglobin, increased markers of haemolysis and a higher incidence of pulmonary hypertension, priapism and leg ulcers. We compared the patterns of response to regular automated red cell exchange transfusion over a five-year period of 21 patients with recurrent painful crises from the first group and 3 patients with pulmonary hypertension and 5 with recurrent severe stuttering priapism form the second and found them to be distinctly different...
August 18, 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28916313/virulence-of-thermolable-haemolysi-tlh-gastroenteritis-related-pathogenicity-tdh-and-trh-of-the-pathogens-vibrio-parahemolyticus-in-viable-but-non-culturable-vbnc-state
#5
Huamin Zhong, Yukui Zhong, Qiulian Deng, Zhenwen Zhou, Xiaoshan Guan, Muxia Yan, Tingting Hu, Mingyong Luo
In the Viable but Non-Culturable (VBNC) state, microorganisms may survive under severe external environment. In this study, the specificity and sensitivity of PMA-LAMP assay on the detection of Vibrio Parahemolyticus (V. parahemolyticus) has been developed and evaluated, with further application on a number of food-borne V. parahemolyticus strains. Six primers were designed for recognizing 8 distinct targeting on tlh, tdh and trh gene. Through specific penetration through the damaged cell membrane of dead cells and intercalating into DNA, PMA could prevent DNA amplification of dead bacteria from LAMP, which enabled the differentiation of bacteria between VBNC state and dead state...
September 12, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28915106/making-sense-of-a-haemolysis-monitoring-and-reporting-system-a-nationwide-longitudinal-multimethod-study-of-68-australian-laboratory-participant-organisations
#6
Ling Li, Elia Vecellio, Stephanie Gay, Rebecca Lake, Mark Mackay, Leslie Burnett, Douglas Chesher, Stephen Braye, Tony Badrick, Johanna I Westbrook, Andrew Georgiou
BACKGROUND: The key incident monitoring and management systems (KIMMS) quality assurance program monitors incidents in the pre- and postanalytical phases of testing in medical laboratories. Haemolysed specimens have been found to be the most frequent preanalytical error and have major implications for patient care. The aims of this study were to assess the suitability of KIMMS for quality reporting of haemolysis and to devise a meaningful method for reporting and monitoring haemolysis...
September 15, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28912721/physical-and-chemical-processes-and-the-morphofunctional-characteristics-of-human-erythrocytes-in-hyperglycaemia
#7
Victor V Revin, Natalia A Klenova, Natalia V Gromova, Igor P Grunyushkin, Ilia N Solomadin, Alexander Y Tychkov, Anastasia A Pestryakova, Anna V Sadykhova, Elvira S Revina, Ksenia V Prosnikova, Jean-Christophe Bourdon, Nikolai Zhelev
Background: This study examines the effect of graduated hyperglycaemia on the state and oxygen-binding ability of hemoglobin, the correlation of phospholipid fractions and their metabolites in the membrane, the activity of proteolytic enzymes and the morphofunctional state of erythrocytes. Methods: Conformational changes in the molecule of hemoglobin were determined by Raman spectroscopy. The structure of the erythrocytes was analyzed using laser interference microscopy (LIM). To determine the activity of NADN-methemoglobinreductase, we used the P...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28904689/severe-neonatal-cytomegalovirus-infection-about-a-case
#8
Brahim El Hasbaoui, Amal Bousselamti, Mohammed Amine Redouani, Amina Barkat
Maternofoetal infection with Cytomegalovirus (CMV) is the most common congenital infection and a leading cause of mental retardation and sensori-neural hearing loss. Population-based studies indicate that at least 0.5% of all infants born alive have CMV of whom approximately 10% have clinically evident symptomsat birth. The Justification of systematic screening for foetal CMV infection is still controversial and is not recommended in most developed countries. This is mainly justified by the paucity of antenatal prognostic factors and the lack of established intrauterine treatment when foetal infection has been diagnosed...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/28900369/the-local-clinical-validation-of-a-new-lithium-heparin-tube-with-a-barrier-bd-vacutainer%C3%A2-barricor-lh-plasma-tube
#9
Fatma Demet Arslan, Inanc Karakoyun, Banu Isbilen Basok, Merve Zeytinli Aksit, Anil Baysoy, Yasemin Kilic Ozturk, Yusuf Adnan Guclu, Can Duman
INTRODUCTION: Although serum-providing blood tubes with a barrier are still widely used due to their significant advantages, the use of blood tubes with a barrier to provide plasma is becoming widespread. We compared 22 analytes in a BD Vacutainer® Barricor LH Plasma tube for local clinical validation of this new lithium heparin tube with a barrier. MATERIALS AND METHODS: Samples from 44 volunteers were collected in different tubes (Becton Dickinson and Company): Z tube without additive (reference), clot-activator tube with gel (SST), lithium heparin tube without gel (LiH), and lithium heparin tube with barrier (Barricor)...
October 15, 2017: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/28893237/methods-for-the-field-evaluation-of-quantitative-g6pd-diagnostics-a-review
#10
REVIEW
Benedikt Ley, Germana Bancone, Lorenz von Seidlein, Kamala Thriemer, Jack S Richards, Gonzalo J Domingo, Ric N Price
Individuals with glucose-6-phosphate dehydrogenase (G6PD) deficiency are at risk of severe haemolysis following the administration of 8-aminoquinoline compounds. Primaquine is the only widely available 8-aminoquinoline for the radical cure of Plasmodium vivax. Tafenoquine is under development with the potential to simplify treatment regimens, but point-of-care (PoC) tests will be needed to provide quantitative measurement of G6PD activity prior to its administration. There is currently a lack of appropriate G6PD PoC tests, but a number of new tests are in development and are likely to enter the market in the coming years...
September 11, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28883887/indices-of-immune-function-used-by-ecologists-are-mostly-unaffected-by-repeated-freeze-thaw-cycles-and-methodological-deviations
#11
Arne Hegemann, Sara Pardal, Kevin D Matson
BACKGROUND: Over the past couple of decades, measuring immunological parameters has become widespread in studies of ecology and evolution. A combination of different immunological indices is useful for quantifying different parts of the immune system and comprehensively assessing immune function. Running multiple immune assays usually requires samples to be repeatedly thawed and re-frozen. There is some evidence that repeated freezing and thawing can affect assay results, but this has never been comprehensively studied in some common ecological immunology assays...
2017: Frontiers in Zoology
https://www.readbyqxmd.com/read/28875577/biocompatibility-assessment-of-cyclic-olefin-copolymers-impact-of-two-additives-on-cytotoxicity-oxidative-stress-inflammatory-reactions-and-hemocompatibility
#12
Mélisande Bernard, Emile Jubeli, Joudi Bakar, Lionel Tortolano, Johanna Saunier, Najet Yagoubi
This work reports the biocompatibility evaluation of cyclic olefin copolymers (COC) as candidates for implantable medical devices. The focus was to establish the influence of two major additives (antioxidant and lubricant) on the overall biocompatibility. The cytotoxicity was evaluated according to ISO 10993-5 guidelines using L929 fibroblasts, HUVEC and THP-1-derived macrophages. Oxidative stress (ROS, GSH/GSSG and SOD analysis) and pro-inflammatory cytokines (Il-6 and TNF-α secretion) were quantified using THP-1 cells in direct contact with films...
September 5, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28873341/unsaturated-nitrogen-rich-polymer-poly-l-histidine-gated-reversibly-switchable-mesoporous-silica-nanoparticles-using-graft-to-strategy-for-drug-controlled-release
#13
Shengjun Mu, Yongjun Liu, Tianqi Wang, Jing Zhang, Dandan Jiang, Xiaoyue Yu, Na Zhang
A novel and intelligent pH-controlled system having an "on-off" switch based on poly(L-histidine) (PLH) and poly(ethylene glycol) (PEG) coated mesoporous silica nanoparticles (MSNs) (MSNs-PLH-PEG) was designed and evaluated for tumor specific drug release. The unsaturated nitrogen-rich polymer, PLH, which can change its solubility at different pH values, was employed for establishing the reversible "on-off" switch. In vitro drug release results demonstrated that MSNs-PLH-PEG has a pH-controlled "on-off" profile with the change of pH value between pH 7...
September 2, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28870262/virulence-factors-antifungal-susceptibility-and-molecular-mechanisms-of-azole-resistance-among-candida-parapsilosis-complex-isolates-recovered-from-clinical-specimens
#14
Sourour Neji, Ines Hadrich, Houaida Trabelsi, Salma Abbes, Fatma Cheikhrouhou, Hayet Sellami, Fattouma Makni, Ali Ayadi
BACKGROUND: The aim of this study was to determine the biofilm formation, the extracellular enzymatic activities of 182 clinical isolates of the Candida parapsilosis complex. METHODS: Molecular identification of the C. parapsilosis species complex was performed using PCR RFLP of SADH gene and PCR sequencing of ITS region. The susceptibility of ours isolates to antifungal agents and molecular mechanisms underlying azole resistance were evaluated. RESULTS: 63...
September 4, 2017: Journal of Biomedical Science
https://www.readbyqxmd.com/read/28869623/on-influence-of-anodic-oxidation-on-thrombogenicity-and-bioactivity-of-the-ti-13nb-13zr-alloy
#15
Maria Szymonowicz, Alicja Kazek-Kęsik, Maciej Sowa, Bogusława Żywicka, Zbigniew Rybak, Wojciech Simka
PURPOSE: This paper reports on the results of investigations on the blood response to the modified of surfaces titanium alloys. METHODS: To enhance biocompatibility of the Ti-13Nb-13Zr alloy, anodisation was performed at 80 and 150 V. The oxidation process was carried out in a solution containing 4 mol dm-3 H3PO4 and 0.59 mol dm-3 Ca(H2PO2)2. RESULTS: The haemolytic activity of the titanium alloy surface was not altered much by the anodisation...
2017: Acta of Bioengineering and Biomechanics
https://www.readbyqxmd.com/read/28865373/evaluation-of-risk-assessment-of-new-industrial-pollutant-ionic-liquids-on-environmental-living-systems
#16
Viresh R Thamke, Savita R Tapase, Kisan M Kodam
Ionic liquids (ILs) are much known for their promising alternative for volatile solvents in industries and gained popularity as a greener solvent, however industrial effluent discharge containing ILs are also increasing. There is a scarcity of information on the toxicity of ILs; the present study will explore different facts about their harmfulness. The toxic effects of five different ILs: [C4MIM]Br, [Hx3PC14]N(CN)2, [C10MIM]BF4, [BTDA]Cl and [C4MPY]Cl were analysed on bacteria, fungi, plant and animal cells...
August 24, 2017: Water Research
https://www.readbyqxmd.com/read/28857064/implementation-of-g6pd-testing-and-primaquine-for-p-vivax-radical-cure-operational-perspectives-from-thailand-and-cambodia
#17
Suravadee Kitchakarn, Dysoley Lek, Sea Thol, Chantheasy Hok, Aungkana Saejeng, Rekol Huy, Nipon Chinanonwait, Krongthong Thimasarn, Chansuda Wongsrichanalai
Following progressive success in reducing the burden of malaria over the past two decades, countries of the Asia Pacific are now aiming for elimination of malaria by 2030. Plasmodium falciparum and Plasmodium vivax are the two main malaria species that are endemic in the region. P. vivax is generally perceived to be less severe but will be harder to eliminate, owing partly to its dormant liver stage (known as a hypnozoite) that can cause multiple relapses following an initial clinical episode caused by a mosquito-borne infection...
September 2017: WHO South-East Asia Journal of Public Health
https://www.readbyqxmd.com/read/28852484/sickle-cell-disease-and-albuminuria-recent-advances-in-our-understanding-of-sickle-cell-nephropathy
#18
Vincent Audard, Pablo Bartolucci, Thomas Stehlé
Albuminuria is considered to be a relevant biomarker for the detection of early glomerular damage in patients with sickle cell disease (SCD). Improvements in our understanding of the pathophysiological processes and molecular mechanisms underlying albuminuria are required, because increasing numbers of patients with SCD are developing chronic kidney disease. The early recognition of sickle cell nephropathy (SCN) and studies of the natural course of this emerging renal disease are therefore crucial, together with identification of the associated clinical and biological risk factors, to make it possible to initiate kidney-protective therapy at early stages of renal impairment...
August 2017: Clinical Kidney Journal
https://www.readbyqxmd.com/read/28852037/the-g6pd-flow-cytometric-assay-is-a-reliable-tool-for-diagnosis-of-g6pd-deficiency-in-women-and-anaemic-subjects
#19
Germana Bancone, Michael Kalnoky, Cindy S Chu, Nongnud Chowwiwat, Maria Kahn, Benoit Malleret, Pornpimon Wilaisrisak, Laurent Rénia, Gonzalo J Domingo, Francois Nosten
Glucose-6-phosphate dehydrogenase (G6PD) activity is essential for redox equilibrium of red blood cells (RBCs) and, when compromised, the RBCs are more susceptible to haemolysis. 8-aminoquinolines (primaquine and tafenoquine) are used for the radical curative treatment of Plasmodium vivax malaria and can cause haemolysis in G6PD deficient subjects. Haemolytic risk is dependent on treatment dose and patient G6PD status but ultimately it correlates with the number of G6PD deficient RBCs. The G6PD spectrophotometric assay reliably identifies deficient subjects but is less reliable in heterozygous females, especially when other blood conditions are present...
August 29, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28840776/naturally-occurring-antibodies-in-cats-against-dog-erythrocyte-antigens-and-vice-versa
#20
Vito Priolo, Marisa Masucci, Eva Spada, Daniela Proverbio, Maria Grazia Pennisi
Objectives The aim of this study was to investigate the presence of naturally occurring antibodies against canine erythrocyte antigens in cats and vice versa. The influence of canine and feline blood type on cross-match results was also studied. Methods Blood samples from 34 cats and 42 dogs were used to perform test-tube major and minor cross-match tests and blood typing. Blood from each cat was cross-matched with blood from 2-6 dogs, for a total of 111 cross-match tests. Haemolysis, macro- and/or micro-agglutination were considered markers of a positive cross-match...
August 1, 2017: Journal of Feline Medicine and Surgery
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