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wheat Stripe rust

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https://www.readbyqxmd.com/read/29183031/novel-pcr-assays-for-the-detection-of-biological-agents-responsible-for-wheat-rust-diseases-puccinia-triticina-and-puccinia-striiformis-f-sp-tritici
#1
Adam Kuzdraliński, Anna Kot, Hubert Szczerba, Agnieszka Ostrowska, Michał Nowak, Marta Muszyńska, Michał Lechowski, Paweł Muzyka
The species Puccinia triticina (Pt) and Puccinia striiformis f. sp. tritici (Pst) are devastating cereal pathogens that cause leaf and stripe rust diseases. We developed PCR assays for the species-specific detection of Pt and Pst, 2 biological agents that cause wheat rust disease. For each pathogen, we validated 3 primer sets that target the second largest subunits of the RNA polymerase II (rpb2) and β-tubulin 1 (tub1) genes. The specificities of the primers were verified using naturally infected plant materials with visual symptoms of disease...
November 29, 2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29177504/pathogenomic-analysis-of-wheat-yellow-rust-lineages-detects-seasonal-variation-and-host-specificity
#2
Vanessa Bueno-Sancho, Antoine Persoons, Amelia Hubbard, Luis Enrique Cabrera-Quio, Clare M Lewis, Pilar Corredor-Moreno, Daniel C E Bunting, Sajid Ali, Soonie Chng, David P Hodson, Ricardo Madariaga Burrows, Rosie Bryson, Jane Thomas, Sarah Holdgate, Diane G O Saunders
Recent disease outbreaks caused by (re-)emerging plant pathogens have been associated with expansions in pathogen geographic distribution and increased virulence. For example, in the past two decades' wheat yellow (stripe) rust, Puccinia striiformis f. sp. tritici, has seen the emergence of new races that are adapted to warmer temperatures, have expanded virulence profiles, and are more aggressive than previous races, leading to wide-scale epidemics. Here, we used field-based genotyping to generate high-resolution data on P...
November 21, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/29137188/physical-localization-of-a-locus-from-agropyron-cristatum-conferring-resistance-to-stripe-rust-in-common-wheat
#3
Zhi Zhang, Liqiang Song, Haiming Han, Shenghui Zhou, Jinpeng Zhang, Xinming Yang, Xiuquan Li, Weihua Liu, Lihui Li
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat (Triticum aestivum L.) worldwide. Agropyron cristatum (L.) Gaertn. (2n = 28, PPPP), one of the wild relatives of wheat, exhibits resistance to stripe rust. In this study, wheat-A. cristatum 6P disomic addition line 4844-12 also exhibited resistance to stripe rust. To identify the stripe rust resistance locus from A. cristatum 6P, ten translocation lines, five deletion lines and the BC₂F₂ and BC₃F₂ populations of two wheat-A...
November 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29125778/isolation-and-molecular-cytogenetic-characterization-of-a-wheat-leymus-mollis-double-monosomic-addition-line-and-its-progenies-with-resistance-to-stripe-rust
#4
Xiaofei Yang, Xin Li, Changyou Wang, Chunhuan Chen, Zengrong Tian, Wanquan Ji
A common wheat - Leymus mollis (2n = 4x = 28, NsNsXmXm) double monosomic addition line, M11003-4-3-8/13/15 (2n = 44 = 42T.a + L.m2 + L.m3), with stripe rust resistance was developed (where T.a represents Triticum aestivum chromosome, L.m represents L. mollis chromosome, and L.m2/3 represents L. mollis chromosome of homoeologous groups 2 and 3). The progenies of line M11003-4-3-8/13/15 were characterized by cytological observation, specific molecular markers, fluorescence in situ hybridization (FISH), and genomic in situ hybridization (GISH)...
November 10, 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/29070517/host-induced-gene-silencing-of-the-mapkk-gene-psfuz7-confers-stable-resistance-to-wheat-stripe-rust
#5
Xiaoguo Zhu, Tuo Qi, Qian Yang, Fuxin He, Chenglong Tan, Wei Ma, Ralf Thomas Voegele, Zhensheng Kang, Jun Guo
RNA interference (RNAi) is a powerful genetic tool to accelerate research in plant biotechnology and to control biotic stresses by manipulating target gene expression. However, the potential of RNAi in wheat to efficiently and durably control the devastating stripe rust fungus Puccinia striiformis f. sp. tritici (Pst), remained largely under explored, so far. To address this issue, we generated transgenic wheat lines expressing double-stranded RNA targeting PsFUZ7 transcripts of Pst. We analyzed expression of PsFUZ7 and related genes, and resistance traits of the transgenic wheat lines...
October 25, 2017: Plant Physiology
https://www.readbyqxmd.com/read/29067018/molecular-characterization-of-novel-totivirus-like-double-stranded-rnas-from-puccinia-striiformis-f-sp-tritici-the-causal-agent-of-wheat-stripe-rust
#6
Li Zheng, Xia Lu, Xiaofei Liang, Shuchang Jiang, Jing Zhao, Gangming Zhan, Peng Liu, Jianhui Wu, Zhensheng Kang
Characterization of newly isolated mycoviruses may contribute to understanding of the evolution and diversity of viruses. Here, a deep sequencing approach was used to analyze the double-stranded RNA (dsRNA) mycoviruses isolated from field-collected P. striiformis samples in China. Database searches showed the presence of at least four totivirus-like sequences, termed Puccinia striiformis virus 1 to 4 (PsV1 to 4). All of these identified sequences contained two overlapping open reading frames (ORFs) which encode a putative coat protein (CP) and an RNA-dependent RNA polymerase (RdRp) showing similar structures to members of the genus Totivirus...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28965125/rapid-identification-of-an-adult-plant-stripe-rust-resistance-gene-in-hexaploid-wheat-by-high-throughput-snp-array-genotyping-of-pooled-extremes
#7
Jianhui Wu, Shengjie Liu, Qilin Wang, Qingdong Zeng, Jingmei Mu, Shuo Huang, Shizhou Yu, Dejun Han, Zhensheng Kang
High-throughput SNP array analysis of pooled extreme phenotypes in a segregating population by KASP marker genotyping permitted rapid, cost-effective location of a stripe rust resistance QTL in wheat. German wheat cultivar "Friedrichswerther" has exhibited high levels of adult plant resistance (APR) to stripe rust in field environments for many years. F2:3 lines and F6 recombinant inbred line (RILs) populations derived from a cross between Friedrichswerther and susceptible landrace Mingxian 169 were evaluated in the field in 2013, 2016 and 2017...
September 30, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/28952421/genome-wide-association-mapping-of-loci-for-resistance-to-stripe-rust-in-north-american-elite-spring-wheat-germplasm
#8
Jayfred Gaham Godoy, Sheri Rynearson, Xianming Chen, Michael Pumphrey
Stripe rust, caused by Puccinia striiformis Westend. f. sp. tritici Erikss. (Pst), is a major yield-limiting foliar disease of wheat (Triticum aestivum L.) worldwide. In this study, the genetic variability of elite spring wheat germplasm from North America was investigated to characterize the genetic basis of effective all-stage and adult plant resistance (APR) to stripe rust. A genome-wide association study (GWAS) was conducted using 237 elite spring wheat lines genotyped with an Illumina Infinium 90K SNP array...
September 27, 2017: Phytopathology
https://www.readbyqxmd.com/read/28913655/quantitative-trait-loci-for-resistance-to-stripe-rust-of-wheat-revealed-using-global-field-nurseries-and-opportunities-for-stacking-resistance-genes
#9
Firdissa E Bokore, Richard D Cuthbert, Ron E Knox, Harpinder S Randhawa, Colin W Hiebert, Ron M DePauw, Asheesh K Singh, Arti Singh, Andrew G Sharpe, Amidou N'Diaye, Curtis J Pozniak, Curt McCartney, Yuefeng Ruan, Samia Berraies, Brad Meyer, Catherine Munro, Andy Hay, Karim Ammar, Julio Huerta-Espino, Sridhar Bhavani
Quantitative trait loci controlling stripe rust resistance were identified in adapted Canadian spring wheat cultivars providing opportunity for breeders to stack loci using marker-assisted breeding. Stripe rust or yellow rust, caused by Puccinia striiformis Westend. f. sp. tritici Erikss., is a devastating disease of common wheat (Triticum aestivum L.) in many regions of the world. The objectives of this research were to identify and map quantitative trait loci (QTL) associated with stripe rust resistance in adapted Canadian spring wheat cultivars that are effective globally, and investigate opportunities for stacking resistance...
December 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/28881438/host-induced-gene-silencing-of-an-important-pathogenicity-factor-pscpk1-in-puccinia-striiformis-f-sp-tritici-enhances-resistance-of-wheat-to-stripe-rust
#10
Tuo Qi, Xiaoguo Zhu, Chenlong Tan, Peng Liu, Jia Guo, Zhensheng Kang, Jun Guo
Rust fungi are devastating plant pathogens and cause a large economic impact on wheat production worldwide. To overcome this rapid loss of resistance in varieties, we generated stable transgenic wheat plants expressing short interfering RNAs (siRNAs) targeting potentially vital genes of Puccinia striiformis f. sp. tritici (Pst). Protein kinase A (PKA) has been proved to play important roles in regulating the virulence of phytopathogenic fungi. PsCPK1, a PKA catalytic subunit gene from Pst, is highly induced at the early infection stage of Pst...
September 7, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28878791/characterization-and-mapping-of-leaf-rust-and-stripe-rust-resistance-loci-in-hexaploid-wheat-lines-uc1110-and-pi610750-under-mexican-environments
#11
Caixia Lan, Iago L Hale, Sybil A Herrera-Foessel, Bhoja R Basnet, Mandeep S Randhawa, Julio Huerta-Espino, Jorge Dubcovsky, Ravi P Singh
Growing resistant wheat varieties is a key method of minimizing the extent of yield losses caused by the globally important wheat leaf rust (LR) and stripe rust (YR) diseases. In this study, a population of 186 F8 recombinant inbred lines (RILs) derived from a cross between a synthetic wheat derivative (PI610750) and an adapted common wheat line (cv. "UC1110") were phenotyped for LR and YR response at both seedling and adult plant stages over multiple seasons. Using a genetic linkage map consisting of single sequence repeats and diversity arrays technology markers, in combination with inclusive composite interval mapping analysis, we detected a new LR adult plant resistance (APR) locus, QLr...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28876207/inheritance-of-virulence-construction-of-a-linkage-map-and-mapping-dominant-virulence-genes-in-puccinia-striiformis-f-sp-tritici-through-characterization-of-a-sexual-population-with-genotyping-by-sequencing
#12
Congying Yuan, Meinan Wang, Daniel Z Skinner, Deven R See, Chongjing Xia, Xinhong Guo, Xianming Chen
Puccinia striiformis f. sp. tritici (Pst), the wheat stripe rust pathogen, is a dikaryotic, biotrophic, and macrocyclic fungus. Genetic study of Pst virulence was not possible until the recent discovery of Berberis spp. and Mahonia spp. as alternate hosts. To determine inheritance of virulence and map virulence genes, a segregating population of 119 isolates was developed by self-fertilizing Pst isolate 08-220 (race PSTv-11) on barberry leaves under controlled greenhouse conditions. The progeny isolates were phenotyped on a set of 29 wheat lines with single genes for race-specific resistance and genotyped with simple sequence repeat (SSR) markers, simple sequence repeat derived from secreted protein genes (SP-SNP), and SNP markers from genotyping- by-sequencing (GBS)...
September 6, 2017: Phytopathology
https://www.readbyqxmd.com/read/28856657/bsmv-induced-gene-silencing-assay-for-functional-analysis-of-wheat-rust-resistance
#13
Li Huang
Virus-induced gene silencing (VIGS) is a widely used reverse genetics tool to knock down genes in plants transiently without transformation. The assay has been successfully used to downregulate the transcript abundance of a target gene at almost any plant developmental stages in any tissues. Here, we describe the VIGS assay using a barley stripe mosaic virus (BSMV) for functional genomics analysis in wheat with the focus on genes involved in rust resistance.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28856647/genetic-analysis-of-resistance-to-wheat-rusts
#14
Caixia Lan, Mandeep Singh Randhawa, Julio Huerta-Espino, Ravi P Singh
Leaf rust, stripe rust, and stem rust pose a significant threat to global wheat production. Growing rust resistant cultivars is the most efficient and environment friendly method to reduce yield losses. Genetic analysis is undertaken to identify genes and study their roles in conferring rust resistance in a given wheat background. This chapter summarizes the protocol for genetic analysis of rust resistance at both seedling and adult plant stages. Additionally, it examines statistical analysis and related software to characterize quantitative trait loci (QTL) linked with rust resistance...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28856645/investigating-gene-function-in-cereal-rust-fungi-by-plant-mediated-virus-induced-gene-silencing
#15
Vinay Panwar, Guus Bakkeren
Cereal rust fungi are destructive pathogens, threatening grain production worldwide. Targeted breeding for resistance utilizing host resistance genes has been effective. However, breakdown of resistance occurs frequently and continued efforts are needed to understand how these fungi overcome resistance and to expand the range of available resistance genes. Whole genome sequencing, transcriptomic and proteomic studies followed by genome-wide computational and comparative analyses have identified large repertoire of genes in rust fungi among which are candidates predicted to code for pathogenicity and virulence factors...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28856638/race-typing-of-puccinia-striiformis-on-wheat
#16
Mogens S Hovmøller, Julian Rodriguez-Algaba, Tine Thach, Chris K Sørensen
A procedure for virulence phenotyping of isolates of yellow (stripe) rust using spray inoculation of wheat seedlings by spores suspended in an engineered fluid, Novec™ 7100, is presented. Differential sets consisting of near-isogenic Avocet lines, selected lines from the "World" and "European" sets, and additional varieties showing race-specificity facilitate a robust assessment of race, irrespectively of geographical and evolutionary origin of isolates. A simple procedure for purification of samples consisting of multiple races is also presented...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28832276/combining-snp-genotyping-array-with-bulked-segregant-analysis-to-map-a-gene-controlling-adult-plant-resistance-to-stripe-rust-in-wheat-line-03031-1-5-h62
#17
Jianhui Wu, Qilin Wang, Liangsheng Xu, Xianming Chen, Bei Li, Jingmei Mu, Qingdong Zeng, Lili Huang, Dejun Han, Zhensheng Kang
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating diseases of wheat worldwide. Growing resistant cultivars is considered the best approach to manage this disease. In order to identify the resistance gene(s) in wheat line 03031-1-5 H62, which displayed high resistance to stripe rust at adult plant stage, a cross was made between 03031-1-5 H62 and susceptible cultivar Avocet S. The mapping population was tested with Chinese Pst race CYR32 through artificial inoculation in a field in Yangling, Shaanxi Province and in Tianshui, Gansu Province under natural infection...
August 23, 2017: Phytopathology
https://www.readbyqxmd.com/read/28798752/qtl-analysis-and-nested-association-mapping-for-adult-plant-resistance-to-powdery-mildew-in-two-bread-wheat-populations
#18
Yan Ren, Weixiu Hou, Caixia Lan, Bhoja R Basnet, Ravi P Singh, Wei Zhu, Xiyong Cheng, Dangqun Cui, Feng Chen
CIMMYT wheat (Triticum aestivum L.) lines Francolin#1 and Quaiu#3 displayed effective and stable adult plant resistance (APR) to Chinese Blumeria graminis f. sp. tritici isolates in the field. To elucidate their genetic basis of resistance, two recombinant inbred line (RIL) populations of their crosses with Avocet, the susceptible parent, were phenotyped in Zhengzhou and Shangqiu in the 2014-2015 and 2015-2016 cropping seasons. These populations were also genotyped with SSR (simple sequence repeat markers) and DArT (diversity arrays technology) markers...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28770301/genome-wide-association-mapping-reveals-a-rich-genetic-architecture-of-stripe-rust-resistance-loci-in-emmer-wheat-triticum-turgidum-ssp-dicoccum
#19
Weizhen Liu, Marco Maccaferri, Xianming Chen, Gaetano Laghetti, Domenico Pignone, Michael Pumphrey, Roberto Tuberosa
SNP-based genome scanning in worldwide domesticated emmer germplasm showed high genetic diversity, rapid linkage disequilibrium decay and 51 loci for stripe rust resistance, a large proportion of which were novel. Cultivated emmer wheat (Triticum turgidum ssp. dicoccum), one of the oldest domesticated crops in the world, is a potentially rich reservoir of variation for improvement of resistance/tolerance to biotic and abiotic stresses in wheat. Resistance to stripe rust (Puccinia striiformis f. sp. tritici) in emmer wheat has been under-investigated...
November 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/28769954/taarpc3-contributes-to-wheat-resistance-against-the-stripe-rust-fungus
#20
Tuo Qi, Juan Wang, Qixiong Sun, Brad Day, Jun Guo, Qing Ma
The actin cytoskeleton participates in numerous cellular processes, including less-characterized processes, such as nuclear organization, chromatin remodeling, transcription, and signal transduction. As a key regulator of actin cytoskeletal dynamics, the actin related protein 2/3 complex (Arp2/3 complex) controls multiple developmental processes in a variety of tissues and cell types. To date, the role of the Arp2/3 complex in plant disease resistance signaling is largely unknown. Herein, we identified and characterized wheat ARPC3, TaARPC3, which encodes the C3 subunit of the Arp2/3 complex...
2017: Frontiers in Plant Science
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