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https://www.readbyqxmd.com/read/28208254/electrochemical-detection-of-escherichia-coli-from-aqueous-samples-using-engineered-phages
#1
Danhui Wang, Juhong Chen, Sam R Nugen
In this study, an enzyme-based electrochemical method was developed for the detection of Escherichia coli (E. coli) using the T7 bacteriophages engineered with lacZ operon encoding for beta-galactosidase (β-gal). The T7lacZ phages can infect E. coli, and have the ability to trigger the overexpression of β-gal during the infection of E. coli. The use of the engineered phages resulted in a more sensitive detection of E. coli by (1) overexpression of β-gal in E. coli during the specific infection and (2) release of the endogenous intracellular β-gal from E...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28195398/preparation-of-magnetic-mesoporous-silica-composite-for-the-solid-phase-microextraction-of-diazinon-and-malathion-before-their-determination-by-high-performance-liquid-chromatography
#2
Alireza Bazmandegan-Shamili, Ali Mohammad Haji Shabani, Shayessteh Dadfarnia, Masoud Rohani Moghadam, Mahboubeh Saeidi
A novel magnetic mesoporous silica material was synthesized and used as the sorbent for the magnetic solid-phase microextraction of diazinon and malathion before their quantification by high-performance liquid chromatography with UV detection. The sorbent was synthesized by a surfactant-templated one-pot sol-gel procedure using SiO2 -coated Fe3 O4 as the magnetic support, cetyltrimethylammonium bromide as the template and tetraethyl orthosilicate as the silicon source. The characteristics of the prepared sorbent were investigated using FTIR spectroscopy, scanning electron microscopy, and X-ray diffraction...
February 13, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28194931/influence-of-scaffold-design-on-3d-printed-cell-constructs
#3
Auryn Souness, Fernanda Zamboni, Gavin M Walker, Maurice N Collins
Additive manufacturing is currently receiving significant attention in the field of tissue engineering and biomaterial science. The development of precise, affordable 3D printing technologies has provided a new platform for novel research to be undertaken in 3D scaffold design and fabrication. In the past, a number of 3D scaffold designs have been fabricated to investigate the potential of a 3D printed scaffold as a construct which could support cellular life. These studies have shown promising results; however, few studies have utilized a low-cost desktop 3D printing technology as a potential rapid manufacturing route for different scaffold designs...
February 14, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28194874/preparation-and-characterization-of-a-silk-fibroin-calcium-sulfate-bone-cement
#4
Fuzhan Zhang, Hai Zhu, Genlin Wang, Jile Xie, Yunxia Tao, Wei Xia, Huilin Yang
This study aimed to prepare and characterize a silk fibroin/calcium sulfate (SF/CS) bone cement. SF solutions of the following concentrations 3, 4.5, 6, 7.5, and 9 g/L were used to prepare bone cement samples with SF-to-CS ratios of 0.35, 0.40, 0.45, 0.50, and 0.55 mL of SF solutions (or water as control) per g of CS. Compressive strength, setting time, degradation when immersed in phosphate-buffered saline, X-ray diffraction, Fourier infrared spectroscopy, and scanning electron microscopy were used to characterize the SF/CS bone cement...
February 14, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28174579/overexpression-of-mpcys4-a-phytocystatin-gene-from-malus-prunifolia-willd-borkh-enhances-stomatal-closure-to-confer-drought-tolerance-in-transgenic-arabidopsis-and-apple
#5
Yanxiao Tan, Mingjun Li, Yingli Yang, Xun Sun, Na Wang, Bowen Liang, Fengwang Ma
Phytocystatins (PhyCys) comprise a group of inhibitors for cysteine proteinases in plants. They play a wide range of important roles in regulating endogenous processes and protecting plants against various environmental stresses, but the underlying mechanisms remain largely unknown. Here, we detailed the biological functions of MpCYS4, a member of cystatin genes isolated from Malus prunifolia. This gene was activated under water deficit, heat (40°C), exogenous abscisic acid (ABA), or methyl viologen (MV) (Tan et al...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28159244/preparation-of-steppogenin-and-ascorbic-acid-vitamin-e-butylated-hydroxytoluene-oil-in-water-microemulsions-characterization-stability-and-antibrowning-effects-for-fresh-apple-juice
#6
Jing Tao, Qin Zhu, Fang Qin, Mingfu Wang, Jie Chen, Zong-Ping Zheng
Oil-in-water microemulsions (O/W MEs) allow the preparation of insoluble compounds into liquid. In this study, we prepared O/W MEs to improve the solubility and stability of steppogenin (S) in aqueous liquid, and studied their ability to inhibit fresh apple juice browning. The ME technique greatly increased steppogenin solubility up to 3000-fold higher than that in water. All SMEs demonstrated good stability after acceleration and long-term storage. In particular, 0.01% SME was associated with dramatic inhibition of fresh apple juice browning after 24h at room temperature and 7days at 4°C, and its antibrowning effects were further improved when combined with 0...
June 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28117157/direct-competitive-fluoroimmunoassays-for-detection-of-imidaclothiz-in-environmental-and-agricultural-samples-using-quantum-dots-and-europium-as-labels
#7
Xiude Hua, Yuan Ding, Jiachuan Yang, Ming Ma, Haiyan Shi, Minghua Wang
A direct quantum dots-based fluoroimmunoassay (QDFIA) and a time-resolved fluoroimmunoassay (TRFIA) for imidaclothiz (IMI) were developed by using the quantum dots (QDs)-labeled antibody and the europium (Eu(3+))-labeled antibody, respectively. After optimization, the half-maximal inhibition concentration (IC50) and the limit of detection (LOD, IC10) are 20.41 and 0.52μgL(-1) for the QDFIA, while 6.91 and 0.018μgL(-1) for the TRFIA, respectively. The cross-reactivities (CRs) with the analogues are negligible except for imidacloprid with CRs of 29...
January 20, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28101467/some-qualitative-and-rheological-properties-of-virgin-olive-oil-apple-vinegar-salad-dressing-stabilized-with-xanthan-gum
#8
Solmaz Abedinzadeh, Mohammadali Torbati, Sodeif Azadmard-Damirchi
Purpose: Lipid oxidation and rheological properties are the main qualitative parameters determined in food emulsions. Salad dressings are food emulsions important in our daily diet, but conventional salad dressings have high amounts of cholesterol and saturated fatty acids because of egg yolk in their formulations. There are many studies on the modification of salad dressing formulations to replace egg yolk and saturated fats. The present study describes new formulation of salad dressing with olive oil and apple vinegar to produce a functional food product...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28089203/carbon-footprint-and-energy-use-of-food-waste-management-options-for-fresh-fruit-and-vegetables-from-supermarkets
#9
Mattias Eriksson, Johanna Spångberg
Food waste is a problem with economic, environmental and social implications, making it both important and complex. Previous studies have addressed food waste management options at the less prioritised end of the waste hierarchy, but information on more prioritised levels is also needed when selecting the best available waste management options. Investigating the global warming potential and primary energy use of different waste management options offers a limited perspective, but is still important for validating impacts from the waste hierarchy in a local context...
January 11, 2017: Waste Management
https://www.readbyqxmd.com/read/28042104/laboratory-bioassays-with-three-different-substrates-to-test-the-efficacy-of-insecticides-against-various-stages-of-drosophila-suzukii-diptera-drosophilidae
#10
Aneliya Koleva Pavlova, Melanie Dahlmann, Mirjam Hauck, Annette Reineke
Rapid worldwide spread and polyphagous nature of the spotted wing Drosophila Drosophila suzukii Matsumura (Diptera: Drosophilidae) calls for efficient and selective control strategies to prevent severe economic losses in various fruit crops. The use of insecticides is one option for management of this invasive pest insect. Efficacy of insecticides is usually assessed first in laboratory bioassays, which are compounded by the cryptic nature of D. suzukii larvae and the fact that fruits used in bioassays often start to rot and dissolve before larvae have reached the adult stage...
January 2017: Journal of Insect Science
https://www.readbyqxmd.com/read/28008718/binary-solvent-based-ionic-liquid-assisted-surfactant-enhanced-emulsification-microextraction-for-the-determination-of-four-fungicides-in-apple-juice-and-apple-vinegar
#11
Xiaochu Chen, Xu Zhang, Fengmao Liu, Fan Hou
A binary-solvent-based ionic-liquid-assisted surfactant-enhanced emulsification microextraction method was developed for the separation/preconcentration and determination of four fungicides (pyrimethanil, fludioxonil, cyprodynil, pyraclostrobin) in apple juice and apple vinegar. A nonchlorinated solvent amyl acetate, which has a lower density than water, was used as the extraction solvent, and an ionic liquid 1-hexyl-3-methylimidazolium hexafluorophosphate, which has a high density and low toxicity, was used as a secondary solvent mixed with the extraction solvent...
December 23, 2016: Journal of Separation Science
https://www.readbyqxmd.com/read/27993542/modification-of-citrus-and-apple-pectin-by-power-ultrasound-effects-of-acid-and-enzymatic-treatment
#12
Nerea Muñoz-Almagro, Antonia Montilla, F Javier Moreno, Mar Villamiel
Pectin-derived oligosaccharides are emerging as a new generation of functional ingredients with new or improved technological and/or bioactive properties as compared to pectin. This work addresses the impact of power ultrasound (US) on the structure of citrus and apple pectin under different experimental conditions of power, amplitude and pectin concentration in aqueous and acid media, as well as in the presence of a pectinase. Results indicated that depolymerisation of both pectin increased with time and intensity of US in aqueous media and their polydispersity decreased...
December 1, 2016: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/27987275/self-assembled-magnetic-nanoparticle-supported-zeolitic-imidazolate-framework-8-an-efficient-adsorbent-for-the-enrichment-of-triazine-herbicides-from-fruit-vegetables-and-water
#13
Lian Zhou, Ping Su, Yulan Deng, Yi Yang
Zeolitic imidazolate frameworks have positive surface charges and high adsorption capabilities. In this work, zeolitic imidazolate frameworks-8 and negatively charged magnetic nanoparticles were self-assembled by electrostatic attraction under sonication. The extraction performance of the synthesized hybrid material was evaluated by using it as a magnetic adsorbent for the enrichment of triazine herbicides in various sample matrices prior to analysis using ultrafast liquid chromatography. The main parameters, that is, extraction time, adsorbent dosage, salt concentration, and desorption conditions, were evaluated...
December 16, 2016: Journal of Separation Science
https://www.readbyqxmd.com/read/27977925/atomic-layer-deposition-of-in2o3-h-from-incp-and-h2o-o2-microstructure-and-isotope-labeling-studies
#14
Yizhi Wu, Bart Macco, Dries Vanhemel, Sebastian Kölling, Marcel A Verheijen, Paul M Koenraad, Wilhelmus M M Kessels, Fred Roozeboom
The atomic layer deposition (ALD) process of hydrogen-doped indium oxide (In2O3:H) using indium cyclopentadienyl (InCp) and both O2 and H2O as precursors is highly promising for the preparation of transparent conductive oxides. It yields a high growth per cycle (>0.1 nm), is viable at temperatures as low as 100 °C, and provides a record optoelectronic quality after postdeposition crystallization of the films ( ACS Appl. Mat. Interfaces , 2015 , 7 , 16723 - 16729 , DOI: 10.1021/acsami.5b04420 ) . Since both the dopant incorporation and the film microstructure play a key role in determining the optoelectronic properties, both the crystal growth and the incorporation of the hydrogen dopant during this ALD process are studied in this work...
December 27, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27928532/collagen-extracted-from-persian-gulf-squid-exhibits-anti-cytotoxic-properties-on-apple-pectic-treated-cells-assessment-in-an-in-vitro-bioassay-model
#15
Ladan Delphi, Houri Sepehri, Elaheh Motevaseli, Mohammad Reza Khorramizadeh
BACKGROUND: Collagen-based three-dimensional (3D) in vitro systems have been introduced to study the physiological states of cells. As a biomolecule, collagen is usually extracted from terrestrial animals whilst aquatic animals like squid contain large amounts of collagen. METHODS: In order to make effective use of marine organisms, we selected Persian Gulf squid in 2015 to extract the required collagen. Then, a 3D culture system based on the extracted collagen was applied to investigate cellular mechanisms in a native microenvironment...
August 2016: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/27917839/characterization-and-1-57%C3%A2-%C3%A3-resolution-structure-of-the-key-fire-blight-phosphatase-amsi-from-erwinia-amylovora
#16
Marco Salomone-Stagni, Francesco Musiani, Stefano Benini
AmsI is a low-molecular-weight protein tyrosine phosphatase that regulates the production of amylovoran in the Gram-negative bacterium Erwinia amylovora, a specific pathogen of rosaceous plants such as apple, pear and quince. Amylovoran is an exopolysaccharide that is necessary for successful infection. In order to shed light on AmsI, its structure was solved at 1.57 Å resolution at the same pH as its highest measured activity (pH 5.5). In the active site, a water molecule, bridging between the catalytic Arg15 and the reaction-product analogue sulfate, might be representative of the water molecule attacking the phospho-cysteine intermediate in the second step of the reaction mechanism...
December 1, 2016: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/27889932/interaction-of-hepes-buffer-with-glass-ceramic-scaffold-can-hepes-replace-tris-in-sbf
#17
Dana Rohanová, Diana Horkavcová, Laine Paidere, Aldo Roberto Boccaccini, Pavlína Bozděchová, Petr Bezdička
An international standard (ISO: 23317:2014) exists for the in vitro testing of inorganic biomaterials in simulated body fluid (SBF). This standard uses TRIS buffer to maintain neutral pH in SBF, but in our previous paper, we showed that the interaction of a tested glass-ceramic material with TRIS can produce false-positive results. In this study, we evaluated whether the HEPES buffer, which also belongs to the group of Good's buffers, would be more suitable for SBF. We compared its suitability in two media: SBF with HEPES and demineralized water with HEPES...
November 27, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/27886447/synthesis-of-novel-quaternary-silica-hybrid-bioactive-microspheres
#18
A Angelopoulou, E Κ Efthimiadou, G Kordas
PURPOSE: To survey the preparation of novel hybrid microspheres of quaternary silicate glassy composition (SiO2 P2 O5 CaONa2 O) and the prospect of using them as an osteogenic system with enhanced bioactive properties for the development of hydroxyapatite. METHOD: In line with our previous synthetic procedure a two-step process was followed, wherein polystyrene (PS) microspheres were prepared by the emulsifier free-emulsion polymerization method and constituted the core for the sol-gel coating of the silicate inorganic shell...
November 25, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/27882892/automated-online-solid-phase-extraction-liquid-chromatography-mass-spectrometric-analysis-of-dithianon-in-water
#19
Alice Passoni, Marco Vighi, Enrico Davoli, Roberto Fanelli, Renzo Bagnati
An automated online solid-phase extraction-liquid chromatography mass spectrometry (SPE-LC/MS) method was developed for the quantification of dithianon in surface water samples, using warfarin as internal standard. The method was developed on a liquid chromatography (LC) system with Flexible Cube interfaced to a quadrupole time-of-flight (Q-TOF) mass spectrometer. A small volume of acidified water (1 mL) was spiked with internal standard, pre-concentrated online on polymeric cartridges and analyzed by full-scan MS in high-resolution conditions...
2016: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/27882564/processing-black-mulberry-into-jam-effects-on-antioxidant-potential-and-in-vitro-bioaccessibility
#20
Merve Tomas, Gamze Toydemir, Dilek Boyacioglu, Robert D Hall, Jules Beekwilder, Esra Capanoglu
BACKGROUND: Black mulberries (Morus nigra) were processed into jam on an industrialized scale, including the major steps of: selection of frozen black mulberries, adding glucose-fructose syrup and water, cooking, adding citric acid and apple pectin, removing seeds, and pasteurization. Qualitative and quantitative determinations of antioxidants in black mulberry samples were performed using spectrophotometric methods, as well as HPLC- and LC-QTOF-MS-based measurements. These analyses included the determination of total polyphenolic content, percent polymeric color, total and individual anthocyanin contents, antioxidant capacity, and in vitro bioaccessibility in processing samples...
November 23, 2016: Journal of the Science of Food and Agriculture
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