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https://www.readbyqxmd.com/read/27917839/characterization-and-1-57%C3%A2-%C3%A3-resolution-structure-of-the-key-fire-blight-phosphatase-amsi-from-erwinia-amylovora
#1
Marco Salomone-Stagni, Francesco Musiani, Stefano Benini
AmsI is a low-molecular-weight protein tyrosine phosphatase that regulates the production of amylovoran in the Gram-negative bacterium Erwinia amylovora, a specific pathogen of rosaceous plants such as apple, pear and quince. Amylovoran is an exopolysaccharide that is necessary for successful infection. In order to shed light on AmsI, its structure was solved at 1.57 Å resolution at the same pH as its highest measured activity (pH 5.5). In the active site, a water molecule, bridging between the catalytic Arg15 and the reaction-product analogue sulfate, might be representative of the water molecule attacking the phospho-cysteine intermediate in the second step of the reaction mechanism...
December 1, 2016: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/27889932/interaction-of-hepes-buffer-with-glass-ceramic-scaffold-can-hepes-replace-tris-in-sbf
#2
Dana Rohanová, Diana Horkavcová, Laine Paidere, Aldo Roberto Boccaccini, Pavlína Bozděchová, Petr Bezdička
An international standard (ISO: 23317:2014) exists for the in vitro testing of inorganic biomaterials in simulated body fluid (SBF). This standard uses TRIS buffer to maintain neutral pH in SBF, but in our previous paper, we showed that the interaction of a tested glass-ceramic material with TRIS can produce false-positive results. In this study, we evaluated whether the HEPES buffer, which also belongs to the group of Good's buffers, would be more suitable for SBF. We compared its suitability in two media: SBF with HEPES and demineralized water with HEPES...
November 27, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/27886447/synthesis-of-novel-quaternary-silica-hybrid-bioactive-microspheres
#3
A Angelopoulou, E Κ Efthimiadou, G Kordas
PURPOSE: To survey the preparation of novel hybrid microspheres of quaternary silicate glassy composition (SiO2 P2 O5 CaONa2 O) and the prospect of using them as an osteogenic system with enhanced bioactive properties for the development of hydroxyapatite. METHOD: In line with our previous synthetic procedure a two-step process was followed, wherein polystyrene (PS) microspheres were prepared by the emulsifier free-emulsion polymerization method and constituted the core for the sol-gel coating of the silicate inorganic shell...
November 25, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/27882892/automated-online-solid-phase-extraction-liquid-chromatography-mass-spectrometric-analysis-of-dithianon-in-water
#4
Alice Passoni, Marco Vighi, Enrico Davoli, Roberto Fanelli, Renzo Bagnati
An automated online solid-phase extraction-liquid chromatography mass spectrometry (SPE-LC/MS) method was developed for the quantification of dithianon in surface water samples, using warfarin as internal standard. The method was developed on a liquid chromatography (LC) system with Flexible Cube interfaced to a quadrupole time-of-flight (Q-TOF) mass spectrometer. A small volume of acidified water (1 mL) was spiked with internal standard, pre-concentrated online on polymeric cartridges and analyzed by full-scan MS in high-resolution conditions...
2016: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/27882564/processing-black-mulberry-into-jam-effects-on-antioxidant-potential-and-in-vitro-bioaccessibility
#5
Merve Tomas, Gamze Toydemir, Dilek Boyacioglu, Robert D Hall, Jules Beekwilder, Esra Capanoglu
BACKGROUND: Black mulberries (Morus nigra) were processed into jam on an industrialized scale, including the major steps of: selection of frozen black mulberries, adding glucose-fructose syrup and water, cooking, adding citric acid and apple pectin, removing seeds, and pasteurization. Qualitative and quantitative determinations of antioxidants in black mulberry samples were performed using spectrophotometric methods, as well as HPLC- and LC-QTOF-MS-based measurements. These analyses included the determination of total polyphenolic content, percent polymeric color, total and individual anthocyanin contents, antioxidant capacity, and in vitro bioaccessibility in processing samples...
November 23, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27881759/effects-of-sea-ice-cover-on-satellite-detected-primary-production-in-the-arctic-ocean
#6
Mati Kahru, Zhongping Lee, B Greg Mitchell, Cynthia D Nevison
The influence of decreasing Arctic sea ice on net primary production (NPP) in the Arctic Ocean has been considered in multiple publications but is not well constrained owing to the potentially large errors in satellite algorithms. In particular, the Arctic Ocean is rich in coloured dissolved organic matter (CDOM) that interferes in the detection of chlorophyll a concentration of the standard algorithm, which is the primary input to NPP models. We used the quasi-analytic algorithm (Lee et al 2002 Appl. Opti...
November 2016: Biology Letters
https://www.readbyqxmd.com/read/27872628/proteomics-and-ssh-analyses-of-ala-promoted-fruit-coloration-and-evidence-for-the-involvement-of-a-mads-box-gene-mdmads1
#7
Xinxin Feng, Yuyan An, Jie Zheng, Miao Sun, Liangju Wang
Skin color is a key quality attribute of fruits and how to improve fruit coloration has long been a major concern. 5-Aminolevulinic acid (ALA), a natural plant growth regulator, can significantly increase anthocyanin accumulation in fruit skin and therefore effectively improve coloration of many fruits, including apple. However, the molecular mechanism how ALA stimulates anthocyanin accumulation in fruit skin remains unknown. Here, we investigated the impact of ALA on apple skin at the protein and mRNA levels...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27871132/aspergillus-penicillioides-differentiation-and-cell-division-at-0-585-water-activity
#8
Andrew Stevenson, Philip G Hamill, Callum J O'Kane, Gerhard Kminek, John D Rummel, Mary A Voytek, Jan Dijksterhuis, John E Hallsworth
Water availability acts as the most-stringent constraint for life on Earth. Thus, understanding the water relations of microbial extremophiles is imperative to our ability to increase agricultural productivity (e.g., by enhancing the processing and turnover of dead organic matter in soils of arid regions); reduce human exposure to mycotoxins in buildings and our food-supply chain; prevent the spoilage of foods/animal feeds, books, museum specimens and artworks; and better control microbiology of industrial fermentations...
November 21, 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/27813104/effect-of-surface-properties-on-colloid-retention-on-natural-and-surrogate-produce-surfaces
#9
Volha Lazouskaya, Taozhu Sun, Li Liu, Gang Wang, Yan Jin
Bacterial contamination of fresh produce is a growing concern in food industry. Pathogenic bacteria can attach to and colonize the surfaces of fresh produce and cause disease outbreaks among consumers. Surface properties of both bacteria and produce affect bacterial contamination; however, the effects of produce roughness, topography, and hydrophobicity on bacterial retention are still poorly understood. In this work, we used spherical polystyrene colloids as bacterial surrogates to investigate colloid retention on and removal (by rinsing) from fresh produce surfaces including tomato, orange, apple, lettuce, spinach, and cantaloupe, and from surrogate produce surface Sharklet (a micro-patterned polymer)...
November 3, 2016: Journal of Food Science
https://www.readbyqxmd.com/read/27807734/lipid-lowering-with-soluble-dietary-fiber
#10
REVIEW
Prasanth Surampudi, Byambaa Enkhmaa, Erdembileg Anuurad, Lars Berglund
Consumption of dietary soluble fibers has been associated with health benefits such as reduced lipid levels, lower blood pressure, improved blood glucose control, weight loss, improved immune function, and reduced inflammation. Many of these health benefits relate to a reduced risk of developing cardiovascular disease. In this paper, we have reviewed recent studies on the hypocholesterolemic effects of dietary soluble fibers as well as fiber-rich foods. Findings include the following: (a) consumption of water-soluble, viscous-forming fibers can reduce total and low-density lipoprotein cholesterol levels by about 5-10 %; (b) minimal changes of high-density lipoprotein cholesterol or triglyceride levels were observed; (c) cholesterol-lowering properties of soluble fibers depend on their physical and chemical properties; and (d) medium to high molecular weight fibers are more effective in reducing lipid levels...
December 2016: Current Atherosclerosis Reports
https://www.readbyqxmd.com/read/27801972/development-of-a-chitosan-based-tissue-engineered-renal-proximal-tubule-conduit
#11
I-Ni Chiang, Wan-Chen Huang, Chao-Yuan Huang, Yeong-Shiau Pu, Tai-Horng Young
Renal proximal tubule cells (RPTCs) are responsible for glomerular filtration and maintenance of water/electrolyte balance. To regenerate a proximal tubule, sufficient cell numbers and normal cell function are requisite. Collagen has been routinely used as a substrate for culturing human RPTCs (HRPTCs); however, the role of biomaterials has not been thoroughly explored. In this study, RPTCs retrieved from human nephrectomy/nephroureterectomy specimens were cultivated on chitosan as a substrate in serum-free condition for up to 150 days...
November 1, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/27776536/postprandial-effect-of-breakfast-glycaemic-index-on-vascular-function-glycaemic-control-and-cognitive-performance-bgi-study-study-protocol-for-a-randomised-crossover-trial
#12
Natalia Sanchez-Aguadero, Luis Garcia-Ortiz, Maria C Patino-Alonso, Sara Mora-Simon, Manuel A Gomez-Marcos, Rosario Alonso-Dominguez, Benigna Sanchez-Salgado, Jose I Recio-Rodriguez
BACKGROUND: Postprandial glycaemic response affects cognitive and vascular function. The acute effect of breakfast glycaemic index on vascular parameters is not sufficiently known. Also, the influence of breakfasts with different glycaemic index on cognitive performance has been mostly studied in children and adolescents with varying results. Therefore, the purpose of this study is to analyse the postprandial effect of high and low glycaemic index breakfasts on vascular function and cognitive performance and their relationship with postprandial glycaemic response in healthy young adults...
October 24, 2016: Trials
https://www.readbyqxmd.com/read/27755580/an-apple-fruit-fermentation-aff-treatment-improves-the-composition-of-the-rhizosphere-microbial-community-and-growth-of-strawberry-fragaria-%C3%A3-ananassa-duch-benihoppe-seedlings
#13
Jie Zhang, Hui Pang, Mengxia Ma, Yufen Bu, Wei Shao, Weijing Huang, Qianlong Ji, Yuncong Yao
Plant growth can be promoted by the application of apple fruit fermentation (AFF), despite unclear of the underlying mechanisms, the effects involved in AFF on rhizosphere microorganisms have been hypothesized. We investigated the consequences of applying AFF alone or in combination with Bacillus licheniformis to strawberry tissue culture seedlings in vitro, the analyses of Denaturing Gradient Gel Electrophoresis (DGGE) and 16S rDNA were performed to determine AFF effects on rhizosphere. Moreover, the growth index and antioxidant enzyme activities were determined 30 days after treatments...
2016: PloS One
https://www.readbyqxmd.com/read/27701814/a-silicone-fiber-coating-as-approach-for-the-reduction-of-fibroblast-growth-on-implant-electrodes
#14
Folke Dencker, Lutz Dreyer, Dietrich Müller, Holger Zernetsch, Gerrit Paasche, Ralf Sindelar, Birgit Glasmacher
In cochlear implant (CI) patients, an increase in electrode impedance due to fibrotic encapsulation is frequently observed. Several attempts have been proposed to reduce fibroblast growth at the electrode contacts, but none proved to be satisfactory so far. Here, a silicone fiber coating of the electrode contacts is presented that provides a complex micro-scale surface topography and increases hydrophobicity to inhibit fibroblast growth and adhesion. A silicone fiber electrospinning process was developed to create a thin and porous fiber mesh...
October 4, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/27660288/soluble-dietary-fiber-ameliorates-radiation-induced-intestinal-epithelial-to-mesenchymal-transition-and-fibrosis
#15
Jianbo Yang, Chao Ding, Xujie Dai, Tengfei Lv, Tingbing Xie, Tenghui Zhang, Wen Gao, Jianfeng Gong, Weiming Zhu, Ning Li, Jieshou Li
BACKGROUND: Intestinal fibrosis is a late complication of pelvic radiotherapy. Epithelial-to-mesenchymal transition (EMT) plays an important role in tissue fibrosis. The aim of this study was to examine the effect of soluble dietary fiber on radiation-induced intestinal EMT and fibrosis in a mouse model. MATERIALS AND METHODS: Apple pectin (4% wt/wt in drinking water) was administered to wild-type and pVillin-Cre-EGFP transgenic mice with intestinal fibrosis induced by a single dose of abdominal irradiation of 10 Gy...
September 22, 2016: JPEN. Journal of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/27653246/genome-wide-identification-and-expression-analysis-of-the-apple-asr-gene-family-in-response-to-alternaria-alternata-f-sp-mali
#16
Kaihui Huang, Yan Zhong, Yingjun Li, Dan Zheng, Zong-Ming Cheng
The ABA/water stress/ripening-induced (ASR) gene family exists universally in higher plants, and many ASR genes are up-regulated during periods of environmental stress and fruit ripening. Although a considerable amount of research has been performed investigating ASR gene response to abiotic stresses, relatively little is known about their roles in response to biotic stresses. In this report, we identified five ASR genes in apple (Malus × domestica) and explored their phylogenetic relationship, duplication events, and selective pressure...
October 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27652928/voice-care-knowledge-by-dysphonic-and-healthy-individuals-of-different-generations
#17
Felipe Moreti, Fabiana Zambon, Mara Behlau
The purpose of this study was to identify the opinions of both dysphonic and vocally healthy individuals regarding the factors that affect their voices positively and negatively, analyzing them according to the generation to which the participants belong. Eight hundred sixty-six individuals (304 dysphonic and 562 vocally healthy; 196 men and 670 women) categorized by generation: 22 individuals in Silent Generation (1926/-/1945), 180 in Baby Boomers (1946/-/1964), 285 in Generation X (1965/-/1981), and 379 in Generation Y (1982/-/2003) responded to two open questions: "Cite five things that you believe are good/bad to your voice"...
July 2016: CoDAS
https://www.readbyqxmd.com/read/27649517/deodorization-of-garlic-breath-by-foods-and-the-role-of-polyphenol-oxidase-and-phenolic-compounds
#18
Rita Mirondo, Sheryl Barringer
Garlic causes a strong garlic breath that may persist for almost a day. Therefore, it is important to study deodorization techniques for garlic breath. The volatiles responsible for garlic breath include diallyl disulfide, allyl mercaptan, allyl methyl disulfide, and allyl methyl sulfide. After eating garlic, water (control), raw, juiced or heated apple, raw or heated lettuce, raw or juiced mint leaves, or green tea were consumed immediately. The levels of the garlic volatiles on the breath were analyzed from 1 to 60 min by selected ion flow tube mass spectrometry (SIFT-MS)...
October 2016: Journal of Food Science
https://www.readbyqxmd.com/read/27615442/apple-pomace-polysaccharides-improves-mitochondrial-function-and-reduces-oxidative-stress-in-the-liver-of-high-fat-diet-induced-obese-mice
#19
Lei Chen, Lei Liu, Caixia Li, ChengLi Hu, Fan Su, Run Liu, Mengqi Zeng, Daina Zhao, Jiankang Liu, Yurong Guo, Jiangang Long
SCOPE: Apple pomace polysaccharide (APP), a free radical scavenger, is one of the major compounds derived from apple pomace. However, whether APP has beneficial effects on metabolic disorders is still unknown. METHODS AND RESULTS: In the present study, water-soluble APP was isolated from the pomace of the locally abundant 'Qinguan' apple and chemically characterized. Then APP was orally administrated to high-fat diet (HFD)-induced obese mice. We found that APP significantly reduced HFD-induced body weight gain and ameliorated HFD-induced hepatic metabolic disorders and oxidative stress...
September 12, 2016: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/27595296/fruit-vinegars-attenuate-cardiac-injury-via-anti-inflammatory-and-anti-adiposity-actions-in-high-fat-diet-induced-obese-rats
#20
Abdenour Bounihi, Arezki Bitam, Asma Bouazza, Lyece Yargui, Elhadj Ahmed Koceir
CONTEXT: Fruit vinegars (FVs) are used in Mediterranean folk medicine for their hypolipidemic and weight-reducing properties. OBJECTIVE: To investigate the preventive effects of three types of FV, commonly available in Algeria, namely prickly pear [Opuntia ficus-indica (L.) Mill (Cectaceae)], pomegranate [Punica granatum L. (Punicaceae)], and apple [Malus domestica Borkh. (Rosaceae)], against obesity-induced cardiomyopathy and its underlying mechanisms. MATERIALS AND METHODS: Seventy-two male Wistar rats were equally divided into 12 groups...
September 5, 2016: Pharmaceutical Biology
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