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Wangbing Zhang, Mei Wei, Wei Song, Yi-Xuan Gong, Xin-An Yang
In this paper, we firstly report the development of a highly sensitive and economical method for accurate analysis of pyridaben residues on fruits based on dual-frequency ultrasonic treatment (DFUT) and flow injection chemiluminescence (CL) detection. The DFUT device is constituted by integrating an ultrasonic bath with an ultrasonic probe. Two quartz glass coils (QGC) with different structures have been designed and applied to evaluate the function of DFUT in the detection process. Recorded data showed that DFUT is an effective method for improving the pyridaben CL signal...
October 10, 2017: Journal of Agricultural and Food Chemistry
Melissa Morselli, Chiara Maria Vitale, Alessio Ippolito, Sara Villa, Roberto Giacchini, Marco Vighi, Antonio Di Guardo
The use of plant protection products (PPPs) in agricultural areas implies potential chemical loadings to surface waters, which can pose a risk to aquatic ecosystems and human health. Due to the spatio-temporal variability of PPP applications and of the processes regulating their transport to surface waters, aquatic organisms are typically exposed to pulses of contaminants. In small mountain watersheds, where runoff fluxes are more rapid due to the steep slopes, such exposure peaks are particularly likely to occur...
October 2, 2017: Science of the Total Environment
Fernanda R Assis, Rui M S C Morais, Alcina M M B Morais
The aim of the present work was to study the effect of the osmotic dehydration (OD) pre-treatment on the mass transfer kinetics and water activity (aw) of apple cubes during hot air drying. The adequacy of different mathematical models to describe the moisture content of the product during this process was also evaluated. Apple cubes were osmotically dehydrated with sucrose or sorbitol solutions at 60 °C, and then dried by air at 25-80 °C. Overall, the OD and rise of the air temperature resulted in an increased water loss rate and a reduction of the aw...
September 2017: Journal of Food Science and Technology
Josep Vicent Mercader, Antonio Abad-Somovilla, Consuelo Agulló, Antonio Abad-Fuentes
Fluxapyroxad is a new-generation carboxamide fungicide, with residues increasingly being found in food samples. Immunochemical assays have gained acceptance in food quality control as rapid, cost-effective, sensitive, and selective methods for large sample throughput and in situ applications. In the present study, immunoreagents to fluxapyroxad were obtained for the first time, and competitive immunoassays were developed for the sensitive and specific determination of fluxapyroxad residues in food samples. Two carboxyl-functionalized analogues of fluxapyroxad were prepared and antibodies with IC50 values in the low nanomolar range were generated from both haptens, though a dissimilar response was observed concerning specificity...
October 4, 2017: Journal of Agricultural and Food Chemistry
Janet van den Boer, Melanie Werts, Els Siebelink, Cees de Graaf, Monica Mars
Choosing foods that require more time to consume and have a low energy density might constitute an effective strategy to control energy intake, because of their satiating capacity. The current study assessed the eating rate of Dutch food, and investigated the associations between eating rate and other food properties. We also explored the opportunities for a diet with a low energy intake rate (kJ/min). Laboratory data on the eating rate of 240 foods-representing the whole Dutch diet-was obtained. The results show a wide variation in both eating rate (from 2 g/min for rice waffle to 641 g/min for apple juice) and energy intake rate (from 0 kJ/min (0 kcal/min) for water to 1766 kJ/min (422 kcal/min) for chocolate milk)...
October 2, 2017: Foods (Basel, Switzerland)
Paulina M Opyd, Adam Jurgoński, Jerzy Juśkiewicz, Joanna Milala, Zenon Zduńczyk, Bogusław Król
Apple pomace includes seeds that are rich in protein, fiber, and oil, which can be extracted from them. However, they can also contain a significant amount of toxigenic amygdalin. We hypothesized that amygdalin is a compound that significantly reduces the nutritional and health quality of defatted apple seeds. An experiment was conducted on rats that were distributed into three groups and fed with high-fructose diets. In the control (C) and amygdalin (AMG) groups, cellulose and casein were the source of dietary fiber and protein, respectively; in the apple seed meal (ASM) group, dietary fiber and protein originated from the endosperm of apple seeds, which were previously defatted and ground...
October 2, 2017: Nutrients
Wei Zheng, Meijuan Wen, Zhiyuan Zhao, Jie Liu, Zhaohui Wang, Bingnian Zhai, Ziyan Li
Water deficit significantly limits dryland rainfed fruit production, so increasing water conservation is crucial for improving fruit productivity in arid and semiarid areas. In this study, we tested two treatments in an apple orchard: 1) PC treatment comprising black plastic mulch (BPM) (in-row) with weed control (inter-row); 2) and PGC treatment comprising BPM (in-row) combined with a summer cover crop (inter-row) of rape (Brassica campestris L.), which was sown in mid-June and was living from July to September...
2017: PloS One
Yun-Peng Bai, Xiao-Jing Luo, Yu-Lian Zhao, Chun-Xiu Li, Dian-Sheng Xu, Jian-He Xu
The biodegradation of pesticides by organophosphorus hydrolases (OPHs) requires an efficient enzyme production technology in industry. Herein, a Pichia pastoris strain was constructed for the extracellular expression of PoOPHM9, an engineered malathion-degrading enzyme. After optimization, the maximum titer and yield of fermentation reached 50.8 kU/L and 4.1 gprotein/L after 3 days, with the highest space-time yield (STY) reported so far, 640 U L(-1) h(-1). PoOPHM9 displayed its high activity and stability in the presence of 0...
October 9, 2017: Journal of Agricultural and Food Chemistry
Weiwei Yu, Min Huang, Jiaojiao Chen, Sizhuo Wu, Kunming Zheng, Song Zeng, Kankan Zhang, Deyu Hu
Residues of dinotefuran and its metabolites, 1-methyl-3-(tetrahydro-3-furylmethyl)urea (UF) and 1-methyl-3-(tetrahydro-3-furylmethyl)guanidine (DN), in apple were investigated using a "QuEChERS" (quick, easy, cheap, effective, rugged, safe) pretreatment and liquid chromatography-tandem mass spectrometry. Limits of detection (LODs) and quantification (LOQs) of dinotefuran, UF, and DN in apples were 0.011-0.960 and 0.037-3.200 μg/kg, respectively. The average recoveries of dinotefuran, UF, and DN in apple ranged from 70...
September 26, 2017: Environmental Monitoring and Assessment
Yirong Guo, Rui Liu, Ying Liu, Dandan Xiang, Yihua Liu, Wenjun Gui, Mingyu Li, Guonian Zhu
The wide application of an organophosphate pesticide triazophos raises concern on the environmental pollution and the potential risk to human health. Thus, it is crucial to regularly monitor triazophos residue in the environment and agro-products. Herein we described a non-competitive immunoassay for trace detection of triazophos using a direct surface plasmon resonance (SPR) biosensor. Two anti-triazophos monoclonal antibodies (mAbs) were immobilized on the sensor chip and characterized by SPR-based kinetic analysis...
September 21, 2017: Science of the Total Environment
Yanqi Qu, Chen Tan, Zhiyun Zhang, Lili He
Herein, a facile solvent mediated silver nanoparticle (AgNP) mirror was introduced as a reproducible and sensitive surface-enhanced Raman scattering (SERS) substrate. The AgNP mirror was fabricated based on commercially available citrate coated AgNPs via a facile and unique water-solvent interface assembly method without using any electrical equipment. By adding 0.2 mg ml(-1) citrate coated AgNPs dropwise into a mediating solvent, self-assembled AgNP mirrors were formed instantly and gradually settled down at the bottom...
September 22, 2017: Analyst
Michael Samsom, Myrto Korogiannaki, Lakshman N Subbaraman, Heather Sheardown, Tannin A Schmidt
Contact lens friction significantly correlates with subjective comfort. Hyaluronan (HA) and proteoglycan 4 (PRG4) are natural boundary lubricants present in the body. The objective of this study was to assess the effect of crosslinked HA into the bulk of model contact lens materials pHEMA, pHEMA/TRIS, and DMAA/TRIS on surface wettability, protein sorption, and boundary lubricating properties at a material-cornea biointerface, both alone and synergistically with PRG4 in solution. Surface wettability was assessed by water contact angle measurement, protein sorption by lysozyme sorption assay, and boundary lubricating properties using an in vitro friction test method...
September 15, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Raha Pourbaghi, Mohammad Zarrebini, Dariush Semnani, Abbasali Pourazar, Nahid Akbari, Reihaneh Shamsfar
Removal of leukocytes from blood products is the most effective means for elimination of undesirable side effects and prevention of possible reactions in recipients. Micro-fibrous mats are currently used for removal of leukocytes from blood. In this study, samples of electrospun nano-fibrous mats were produced. The performance of the produced electrospun nano-fibrous mats as means of leukocytes removal from fresh whole blood was both evaluated and compared with that of commercially available micro-fibrous mats...
September 13, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Huan-San Zhao, Xi-Cun Zhu, Cheng Li, Yu Wei, Geng-Xing Zhao, Yuan-Mao Jiang
The influence of the equidistant sampling method was explored in a hyperspectral model for the accurate prediction of the water content of apple tree canopy. The relationship between spectral reflectance and water content was explored using the sample partition methods of equidistant sampling and random sampling, and a stepwise regression model of the apple canopy water content was established. The results showed that the random sampling model was Y = 0.4797 - 721787.3883 × Z3 - 766567.1103 × Z5 - 771392...
September 11, 2017: Scientific Reports
Xingli Liu, Taihua Mu, Hongnan Sun, Miao Zhang, Jingwang Chen, Marie Laure Fauconnier
The effects of hydrocolloids (hydroxypropylmethylcellulose (HPMC), Carboxymethylcellulose (CMC), xanthan gum (XG), and apple pectin (AP)) at different concentrations on dough thermo-mechanical properties and in vitro starch digestibility of gluten-free potato steamed bread were investigated. Results showed that hydrocolloids addition significantly increased the gelatinization temperature (from 52.0 to 64.2°C) and water absorption (from 56.22 to 66.50%) of potato dough. Moreover, hydrocolloids may be interacted with protein and starch, the density of potato protein bands was decreased by hydrocolloids addition, the reason might be that higher molecular weight complexes might be formed between proteins-hydrocolloids or proteins-proteins, thus change the protein solubility...
January 15, 2018: Food Chemistry
Eugene G Novikov, Victor V Skakun, Jan Willem Borst, Antonie J W G Visser
The Maximum Entropy Method (MEM) was used for the analysis of polarized fluorescence decays of enhanced green fluorescent protein (EGFP) in buffered water/glycerol mixtures, obtained with time-correlated single-photon counting (Visser et al., Methods Appl. Fluoresc. 4 (2016) 035002). To this end, we used a general-purpose software module of MEM that was earlier developed to analyze (complex) laser photolysis kinetics of ligand rebinding reactions in oxygen binding proteins. We demonstrate that the MEM software provides reliable results and is easy to use for the analysis of both total fluorescence decay and fluorescence anisotropy decay of aqueous solutions of EGFP...
August 31, 2017: Methods and Applications in Fluorescence
Robin Guibal, Sophie Lissalde, Julie Leblanc, Karine Cleries, Adeline Charriau, Gaëlle Poulier, Nicolas Mazzella, Jean-Pierre Rebillard, Yoann Brizard, Gilles Guibaud
Two headwaters located in southwest France were monitored for 3 and 2 years (Auvézère and Aixette watershed, respectively) with two sampling strategies: grab and passive sampling with polar organic chemical integrative sampler (POCIS). These watersheds are rural and characterized by agricultural areas with similar breeding practices, except that the Auvézère watershed contains apple production for agricultural diversification and the downstream portion of the Aixette watershed is in a peri-urban area. The agricultural activities of both are extensive, i...
August 26, 2017: Environmental Science and Pollution Research International
Nasrin Taghipour Birgani, Shahla Elhami
A simple and sensitive method was proposed for the preconcentration of trace levels of Al(III) prior to its determination by spectrophotometry, based on dispersive liquid-liquid microextraction. The complexation of the Al(III) was performed by chelation with Eriochrome Cyanine R (ECR). In this method, cetyltrimethyl ammonium bromide (CTAB) as a dispersant was dissolved in chloroform as an extractant solvent, and then the solution was rapidly injected by a syringe into the samples containing Al(III), which had already been complexed by ECR at optimized pH...
January 1, 2017: Journal of AOAC International
Kosei Kawasaki, Yoichi Kamagata
Previously, we reported that when agar is autoclaved with phosphate buffer, H2O2 is formed in the resulting medium (PT) and the colony count on the medium inoculated with environmental samples becomes much lower than that on a medium in which agar and phosphate are autoclaved separately (PS) (Appl Environ Microbiol 80:7659-7666). However, the physicochemical mechanisms underlying this observation remain largely unknown. Here, we determined the factors affecting H2O2 formation in agar. The H2O2 formation was pH-dependent: H2O2 was formed at high concentrations in alkaline or neutral phosphate buffer, but not in acidic one...
August 18, 2017: Applied and Environmental Microbiology
Mina Rajabi, Masoumeh Firouzi, Zahra Hassannejad, Ismaeil Haririan, Payam Zahedi
The peripheral nerve regeneration is still one of the major clinical problems, which has received a great deal of attention. In this study, the electrospun silk fibroin (SF)/poly(ethylene oxide) (PEO) nanofibrous scaffolds were fabricated and functionalized their surfaces with laminin (LN) without chemical linkers for potential use in the peripheral nerve tissue engineering. The morphology, surface chemistry, thermal behavior and wettability of the scaffolds were examined to evaluate their performance by means of scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC) and water contact angle (WCA) measurements, respectively...
August 14, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
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