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https://www.readbyqxmd.com/read/28821549/phosphate-catalyzed-hydrogen-peroxide-formation-from-agar-gellan-and-%C3%AE%C2%BA-carrageenan-and-recovery-of-microorganisms-cultivability-by-catalase-and-pyruvate
#1
Kosei Kawasaki, Yoichi Kamagata
Previously, we reported that when agar is autoclaved with phosphate buffer, H2O2 is formed in the resulting medium (PT) and the colony count on the medium inoculated with environmental samples becomes much lower than that on a medium in which agar and phosphate are autoclaved separately (PS) (Appl Environ Microbiol 80:7659-7666). However, the physicochemical mechanisms underlying this observation remain largely unknown. Here, we determined the factors affecting H2O2 formation in agar. The H2O2 formation was pH-dependent: H2O2 was formed at high concentrations in alkaline or neutral phosphate buffer, but not in acidic one...
August 18, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28805042/fabrication-and-characterization-of-electrospun-laminin-functionalized-silk-fibroin-poly-ethylene-oxide-nanofibrous-scaffolds-for-peripheral-nerve-regeneration
#2
Mina Rajabi, Masoumeh Firouzi, Zahra Hassannejad, Ismaeil Haririan, Payam Zahedi
The peripheral nerve regeneration is still one of the major clinical problems, which has received a great deal of attention. In this study, the electrospun silk fibroin (SF)/poly(ethylene oxide) (PEO) nanofibrous scaffolds were fabricated and functionalized their surfaces with laminin (LN) without chemical linkers for potential use in the peripheral nerve tissue engineering. The morphology, surface chemistry, thermal behavior and wettability of the scaffolds were examined to evaluate their performance by means of scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC) and water contact angle (WCA) measurements, respectively...
August 14, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28796917/single-base-methylome-analysis-reveals-dynamic-epigenomic-differences-associated-with-water-deficit-in-apple
#3
Jidi Xu, Shasha Zhou, Xiaoqing Gong, Yi Song, Steve van Nocker, Fengwang Ma, Qingmei Guan
Cytosine methylation is an essential feature of epigenetic regulation and is involved in various biological processes. Although cytosine methylation has been analyzed at the genomic scale for several plant species, there is a general lack of understanding of the dynamics of global and genic DNA methylation in plants growing in environments challenged with biotic and abiotic stresses. In this study, we mapped cytosine methylation at single-base resolution in the genome of commercial apple (Malus x domestica), and analyzed changes in methylation patterns associated with water deficit in representative drought-sensitive and drought-tolerant cultivars...
August 10, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28783899/development-of-a-phage-chemiluminescent-enzyme-immunoassay-with-high-sensitivity-for-the-determination-of-imidaclothiz-in-agricultural-and-environmental-samples
#4
Yuan Ding, Xiude Hua, Nana Sun, Jiachuan Yang, Jiaqi Deng, Haiyan Shi, Minghua Wang
In this study, we isolated six phage-displayed peptides by biopanning phage-displayed peptide libraries on an immobilized anti-imidaclothiz monoclonal antibody. After analyzing the relative sensitivity of the individual phage-displayed peptides, we subsequently developed and optimized both a phage enzyme immunoassay (P-ELISA) and a phage chemiluminescent enzyme immunoassay (P-CLEIA) to improve the sensitivity and linear range of imidaclothiz assays. The P-CLEIA (50% inhibition concentration (IC50) of 0.86ngmL(-1), linear range of 0...
July 31, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28777094/in-crystallo-activity-tests-with-latent-apple-tyrosinase-and-two-mutants-reveal-the-importance-of-the-mutated-sites-for-polyphenol-oxidase-activity
#5
Ioannis Kampatsikas, Aleksandar Bijelic, Matthias Pretzler, Annette Rompel
Tyrosinases are type 3 copper enzymes that belong to the polyphenol oxidase (PPO) family and are able to catalyze both the ortho-hydroxylation of monophenols and their subsequent oxidation to o-quinones, which are precursors for the biosynthesis of colouring substances such as melanin. The first plant pro-tyrosinase from Malus domestica (MdPPO1) was recombinantly expressed in its latent form (56.4 kDa) and mutated at four positions around the catalytic pocket which are believed to influence the activity of the enzyme...
August 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/28758980/effects-of-pla-film-incorporated-with-zno-nanoparticle-on-the-quality-attributes-of-fresh-cut-apple
#6
Wenhui Li, Lin Li, Yun Cao, Tianqing Lan, Haiyan Chen, Yuyue Qin
A novel nanopackaging film was synthesized by incorporating ZnO nanoparticles into a poly-lactic acid (PLA) matrix, and its effect on the quality of fresh-cut apple during the period of preservation was investigated at 4 ± 1 °C for 14 days. Six wt % cinnamaldehyde was added into the nano-blend film. Scanning electron microscope (SEM) analysis showed a rougher cross-section of the nano-blend films and an X-ray diffraction (XRD) was carried out to determine the structure of the ZnO nanoparticles. Compared to the pure PLA film, the nano-blend film had a higher water vapor permeability (WVP) and lower oxygen permeability...
July 31, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28738607/novel-colorimetric-assay-for-paraquat-detection-on-silica-bead-using-negatively-charged-silver-nanoparticles
#7
Weena Siangproh, Thachkorn Somboonsuk, Orawan Chailapakul, Kriangsak Songsrirote
A simple, rapid, sensitive, and economical method based on colorimetry for the determination of paraquat, a widely used herbicide, was developed. Citrate-coated silver nanoparticles (AgNPs) were synthesized as the colorimetric probe. The mechanism of the assay is related to the aggregation of negatively charged AgNPs as induced by positively-charged paraquat resulting from coulombic attraction which causes the color to change from a deep greenish yellow to pale yellow in accordance with the concentrations of paraquat...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738556/development-of-a-new-sample-preparation-method-based-on-liquid-liquid-liquid-extraction-combined-with-dispersive-liquid-liquid-microextraction-and-its-application-on-unfiltered-samples-containing-high-content-of-solids
#8
Mir Ali Farajzadeh, Maryam Abbaspour
A new sample preparation method based on liquid-liquid-liquid extraction combined with dispersive liquid-liquid microextraction followed by gas chromatography-flame ionization detection has been reported for the extraction/preconcentration and determination of trace levels of twelve pesticide residues from different samples with high content of solids without filtration. This method consists of a three-phase system including an aqueous phase (sample solution), acetonitrile, and hexane. The extraction mechanism is based on different affinities of the substances from the sample matrices towards each of the involved phase, which provides a high selectivity to the process...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28710438/simulation-of-long-term-yield-and-soil-water-consumption-in-apple-orchards-on-the-loess-plateau-china-in-response-to-fertilization
#9
Xingxing Peng, Zheng Guo, Yujiao Zhang, Jun Li
The Loess Plateau, China, is the world's largest apple-producing region, and over 80% of the orchards are in rainfed (dryland) areas. Desiccation of the deep soil layer under dryland apple orchards is the main stressor of apple production in this region. Fertilization is a factor that causes soil desiccation in dryland apple orchards. Given its applicability and precision validations, the Environmental Policy Integrated Climate (EPIC) model was used to simulate the dynamics of fruit yield and deep soil desiccation in apple orchards under six fertilization treatments...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28707217/baseline-glucoregulatory-function-moderates-the-effect-of-dairy-milk-and-fruit-juice-on-postprandial-cognition-in-healthy-young-adults
#10
Jason R Anderson, Misty A W Hawkins, John Updegraff, John Gunstad, Mary Beth Spitznagel
PURPOSE: Few studies have examined acute cognitive effects of dairy products. Prior work suggests baseline glucoregulatory function may moderate the relationship between macronutrient profile and postprandial cognition. This study examined the role of glucoregulatory function in postprandial cognition after milk, fruit juice, and a water control. We hypothesized juice would improve cognition in those with lower fasting glucose, while milk would improve cognition in those with higher fasting glucose...
July 13, 2017: European Journal of Nutrition
https://www.readbyqxmd.com/read/28690262/colorimetric-detection-of-mercury-ii-ion-in-aqueous-solution-using-silver-nanoparticles
#11
M Lutfi Firdaus, Ikka Fitriani, Santhy Wyantuti, Yeni W Hartati, Renat Khaydarov, Jason A McAlister, Hajime Obata, Toshitaka Gamo
Novel green-chemistry synthesis of silver nanoparticles (AgNPs) is introduced as a low-cost, rapid and easy-to-use analytical method for mercury ion detection. Aqueous fruit extract of water apple (Syzygium aqueum) was used for the first time as bioreductant to synthesize stable AgNPs. The prepared AgNPs have a yellowish-brown color with a surface plasmon resonance peak at 420 nm. The addition of Hg(II) ions then changes the AgNPs color to colorless. The color change was in proportion to the concentration of Hg(II) ions...
2017: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/28671837/activated-lignite-based-super-large-granular-slow-release-fertilizers-improve-apple-tree-growth-synthesis-characterizations-and-laboratory-and-field-evaluations
#12
Yafu Tang, Xinying Wang, Yuechao Yang, Bin Gao, Yongshan Wan, Yuncong C Li, Dongdong Cheng
In this work, lignite, a low-grade coal, was modified using the solid-phase activation method with the aid of a Pd/CeO2 nanoparticle catalyst to improve its pore structure and nutrient absorption. Results indicate that the adsorption ability of the activated lignite to NO3(-), NH4(+), H2PO4(-), and K(+) was significantly higher than that of raw lignite. The activated lignite was successfully combined with the polymeric slow-release fertilizer, which exhibits typical slow-release behavior, to prepare the super large granular activated lignite slow-release fertilizer (SAF)...
July 26, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28654874/aquatic-toxicity-of-ivermectin-in-cattle-dung-assessed-using-microcosms
#13
Leticia M Mesa, I Lindt, L Negro, M F Gutierrez, G Mayora, L Montalto, M Ballent, A Lifschitz
Ivermectin (IVM) is a parasiticide widely used for livestock. It is a semisynthetic derivative of avermectin, a macrocyclic lactone produced by Streptomyces avermitilis. This drug is only partly metabolized by livestock; considerable amounts of parent drug are excreted mostly via feces. To simulate exposure of aquatic invertebrates and macrophytes to direct excretion of cattle dung into surface waters, a microcosm experiment with IVM spiked in cattle dung was conducted. The objectives of this study were to characterize accumulation of IVM in water, sediment+dung, roots of the floating fern Salvinia and the zooplankton Ceriodaphnia dubia, the amphipod Hyalella and the apple snail Pomacea; to determine the effect of this drug spiked in cattle dung on life-history traits of these invertebrates; and to evaluate the influence of IVM on aquatic nutrient cycling...
June 24, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28653154/dna-persistence-of-bite-marks-on-food-and-its-relevance-for-str-typing
#14
Céline M Pfeifer, Anja Gass, Rachel Klein-Unseld, Peter Wiegand
In forensic DNA analysis, salivary traces at crime scenes are a promising way to identify a person. However, crime scenes are oftentimes investigated a while after the crime and recovered samples might have been degraded leading to poor PCR amplification. Probably due to decomposition and negative visual impression of spoiled food, bite mark samples make up only a small part of our casework routine. In this study, bite marks on apples and chocolate bars as well as on an inert surface (microscope slide) were stored up to 3 weeks indoors and outdoors during different seasons and analyzed for amylase activity and DNA quantity and quality...
June 26, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28648298/slightly-acidic-electrolyzed-water-combined-with-chemical-and-physical-treatments-to-decontaminate-bacteria-on-fresh-fruits
#15
Charles Nkufi Tango, Imran Khan, Paul-François Ngnitcho Kounkeu, Rubab Momna, Mohammad Shakhawat Hussain, Deog-Hwan Oh
Effect of sequential combination of slightly acidic electrolyzed water (SAEW) with chemical and physical treatments on bacterial decontamination on fruits was investigated in this study. Effect of treatments on microbial and sensory quality was also analyzed after subsequent storage at 4 °C and room temperature (RT, 23 ± 0.15 °C). Whole apple and tomato fruits were inoculated with cocktail strains of Escherichia coli O157:H7 and Listeria monocytogenes. Uninoculated and inoculated fruits were washed first with distilled water (DW), calcium oxide (CaO), fumaric acid (FA), and SAEW at RT for 3 min...
October 2017: Food Microbiology
https://www.readbyqxmd.com/read/28633837/synthesis-and-characterization-of-zns-ni-nps-loaded-on-ac-derived-from-apple-tree-wood-and-their-applicability-for-the-ultrasound-assisted-comparative-adsorption-of-cationic-dyes-based-on-the-experimental-design
#16
Hossein Zare Khafri, Mehrorang Ghaedi, Arash Asfaram, Mohammad Safarpoor
The applicability of ZnS:Ni nanoparticles loaded on activated carbon derived from apple tree wood (ZnS:Ni-NPs-ACATW) for the adsorption of Methylene Blue (MB) and Janus Green B (JGB) dyes in single system from water solution has been described. The synthesized adsorbent characterized and identified by UV-Vis, FE-SEM, EDX, TEM, FTIR and XRD. The influences of operation parameters including initial MB or JGB concentration (9.0-33.0mgL(-1)), pH (4.0-10.0), extent of adsorbent (0.08-0.12g) and sonication time (4...
September 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28632272/interfacial-synthesis-of-a-three-dimensional-hierarchical-mos2-ns-ag-np-nanocomposite-as-a-sers-nanosensor-for-ultrasensitive-thiram-detection
#17
Xiu Liang, Yi-Shuo Wang, Ting-Ting You, Xiao-Juan Zhang, Nan Yang, Guang-Sheng Wang, Peng-Gang Yin
Interfacial self-assembly of ordered nanostructures at oil-water interfaces towards the fabrication of nanofilms has attracted the interest of plenty of scientists, since its discovery in 2004. Herein, further developments have been achieved, and we report a new strategy for the synthesis of a three-dimensional (3D) hierarchical nanostructure, through an interfacial synthesis driven microemulsion process. Thus, the synthesis route has been simplified, with the rigorous experimental conditions of traditional compositing technology...
June 29, 2017: Nanoscale
https://www.readbyqxmd.com/read/28621381/the-methodology-applied-in-dpph-abts-and-folin-ciocalteau-assays-has-a-large-influence-on-the-determined-antioxidant-potential
#18
Helena Abramovič, Blaž Grobin, Nataša Poklar, Blaž Cigić
Antioxidant potential (AOP) is not only the property of the matrix analyzed but also depends greatly on the methodology used. The chromogenic radicals 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+), 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and Folin-Ciocalteu (FC) assay were applied to estimate how the method and the composition of the assay solvent influence the AOP determined for coffee, tea, beer, apple juice and dietary supplements. Large differences between the AOP values depending on the reaction medium were observed, with the highest AOP determined mostly in the FC assay...
June 2017: Acta Chimica Slovenica
https://www.readbyqxmd.com/read/28617325/development-of-biopolymer-composite-films-using-a-microfluidization-technique-for-carboxymethylcellulose-and-apple-skin-particles
#19
Inyoung Choi, Yoonjee Chang, So-Hyang Shin, Eunmi Joo, Hyun Ju Song, Haeyoung Eom, Jaejoon Han
Biopolymer films based on apple skin powder (ASP) and carboxymethylcellulose (CMC) were developed with the addition of apple skin extract (ASE) and tartaric acid (TA). ASP/CMC composite films were prepared by mixing CMC with ASP solution using a microfluidization technique to reduce particle size. Then, various concentrations of ASE and TA were incorporated into the film solution as an antioxidant and an antimicrobial agent, respectively. Fourier transform infrared (FTIR), optical, mechanical, water barrier, and solubility properties of the developed films were then evaluated to determine the effects of ASE and TA on physicochemical properties...
June 15, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28613078/the-impact-of-textile-wastewater-irrigation-on-growth-and-development-of-apple-plant
#20
Hamdi Zenginbal, Gamze Dogdu Okcu, Arda Yalcuk
In this work, the effect of irrigation with textile wastewaters on the growth and development of 'Golden Delicious' apple sapling was examined over a one-year period. Municipal water prepared as a control sample (T0), 1/3 diluted (T1) and undiluted (T2) raw textile wastewater was used as the three different irrigation water samples. Two replications of each test were performed on three random samples each time. When examining the effects of T0, T1 and T2 irrigation water on plant growth, it was found that T1 irrigation water significantly increased the weight, the shoot length and the diameter of the sapling...
June 14, 2017: International Journal of Phytoremediation
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