Tahoura Torkzadeh, Zahra Asadi, Mohammad Jafari Atrabi, Farideh Eivazkhani, Maryam Khodadi, Samira Hajiaghalou, Vahid Akbarinejad, Rouhollah Fathi
CONTEXT: Base medium containing knock-out serum replacement (KSR) has been found to support formation and maintenance of follicles in one-day-old mice ovaries, but has not been shown to properly support activation and growth of primordial follicles. AIMS: The present study was conducted to tailor the hormonal content of base medium containing KSR to enhance development of primordial follicles in neonatal ovaries. METHODS: One-day-old mice ovaries were initially cultured with base medium for four days, and then, different hormonal treatments were added to the culture media and the culture was proceeded for four additional days until day eight...
November 24, 2023: Reproduction, Fertility, and Development