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https://www.readbyqxmd.com/read/29907785/inhibitory-effect-of-slit2-on-granulosa-cell-proliferation-mediated-by-the-cdc42-paks-erk1-2-mapk-pathway-in-the-prehierarchical-follicles-of-the-chicken-ovary
#1
Rifu Xu, Ning Qin, Xiaoxing Xu, Xue Sun, Xiaoxia Chen, Jinghua Zhao
The SLIT2 ligand and ROBO receptors of the SLIT/ROBO pathway are expressed in hen ovarian follicles and have been shown to play critical roles in ovary development, cell proliferation and apoptosis in mammals. However, the exact roles of SLIT2 and the molecular mechanisms of chicken follicle development remain poorly understood. Here, we discovered that high levels of SLIT2 suppress FSHR, GDF9, STAR and CYP11A1 mRNA and protein expression in granulosa cells (GCs) and cell proliferation (p < 0.01). However, these inhibitory effects can be abolished by the siRNA-mediated knockdown of the ROBO1 and ROBO2 receptors...
June 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29902792/xy-sry-positive-ovarian-disorder-of-sex-development-in-cattle
#2
Lisa De Lorenzi, Silvana Arrighi, Elena Rossi, Pierangela Grignani, Carlo Previderè, Stefania Bonacina, Fausto Cremonesi, Pietro Parma
In mammals, the sex of the embryo depends on the SRY gene. In the presence of at least one intact and functional copy of this genetic factor (XY embryo) undifferentiated gonads will develop as testicles that subsequently determine the male phenotype. When this factor is not present, i.e., in subjects with 2 X chromosomes, an alternative pathway induces the development of ovaries, hence a female phenotype. In this case study, we describe a female cattle affected by a disorder of sex development (DSD). The subject, despite having a chromosomal XY constitution, did not develop testicles but ovaries, although they were underdeveloped...
June 13, 2018: Sexual Development: Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation
https://www.readbyqxmd.com/read/29902617/the-possible-ameliorative-effect-of-olea-europaea-l-oil-against-deltamethrin-induced-oxidative-stress-and-alterations-of-serum-concentrations-of-thyroid-and-reproductive-hormones-in-adult-female-rats
#3
Fatiha Mekircha, Samira Chebab, Rosita Gabbianelli, Essaid Leghouchi
This study aimed to verify whether Olea europaea L. (olive) oil (OEO) exerted a protective effect against oxidative stress induced by deltamethrin (DM) and alterations of pituitary, thyroid and gonadal hormones in adult female rats. DM (0,00256 g/kg body weight),OEO (0,6 g/kg body weight) and DM with OEO were administered to rats orally for 28 days. Volatile compounds present in olive oil were analysed by GC-MS. Estradiol (E2 ), Thyroxine (T4),Thyroid Stimulating Hormone (TSH), Triiodothyronine (T3), Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH), Progesterone (Pg) were measured in serum using Chemiluminescent Microparticle Immunoassay (CMIA)...
June 11, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29898482/-sonographic-imaging-of-physiological-ovaries-in-the-dog
#4
Theresa Conze, Axel Wehrend
Ultrasonography of the canine ovary is a non-invasive examination procedure, which is an important part of the gynaecological examination. For the correct visualisation of the canine ovary, practical experience as well as the correct equipment are important. This article describes the technical requirements, the examination procedure and the appearance of the physiological ovary. The examination can be performed on both a recumbent and standing dog. To find the ovaries, the kidneys can be used as a reference point...
June 2018: Tierärztliche Praxis. Ausgabe K, Kleintiere/Heimtiere
https://www.readbyqxmd.com/read/29897931/spatial-distribution-of-preantral-follicles-in-the-equine-ovary
#5
Benner G Alves, Kele A Alves, Gustavo D A Gastal, Melba O Gastal, José R Figueiredo, Eduardo L Gastal
Comprehensive studies on spatial distribution of preantral follicles in the ovary are scarce. Considering that preantral follicles represent the main ovarian reserve, harvesting of these follicles is crucial for the development/use of assisted reproductive techniques. Therefore, knowledge on follicle spatial distribution can be helpful for targeting areas with richer number of preantral follicles through biopsy procedures. The aim of this study was to assess the distribution and localization of equine preantral follicles according to: (i) age, (ii) ovarian portion (lateral and intermediary) and region (dorsal and ventral), (iii) distance from the geometric center, and (iv) follicular class...
2018: PloS One
https://www.readbyqxmd.com/read/29896242/expression-profiling-of-lipocalin-2-and-24p3-receptor-in-murine-gonads-at-different-developmental-stages
#6
Elsa De La Chesnaye, Leticia Manuel-Apolinar, Leticia Damasio, Aleida Olivares, Miguel Angel Palomino, Isis Santos, Juan Pablo Méndez
Numerous clinical studies have reported the association between high circulating levels of lipocalin-2 (LCN2) and metabolic diseases. However, only few studies have addressed sexually dimorphic, either in its circulating concentration or in its expression in other organs. To the best of our knowledge, LCN2 and the 24p3 receptor (24p3R), have not been identified in gonads; therefore, the present study analyzed their mRNA expression profile and cellular localization in gonads collected from fetal rats at 21 days post coitum, as well as from neonatal rats at 0, 2, 4, 6, 12, 20 and 30 postnatal days...
July 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29893313/comparing-surgical-outcome-and-ovarian-reserve-after-laparoscopic-hysterectomy-between-two-methods-of-with-and-without-prophylactic-bilateral-salpingectomy-a-randomized-controlled-trial
#7
Zahra Asgari, Afsaneh Tehranian, Safoura Rouholamin, Reihaneh Hosseini, Mahdi Sepidarkish, Mahroo Rezainejad
Background: This study aimed to compare the surgical outcome and ovarian reserve in premenopausal women undergoing laparoscopic hysterectomy (without oophorectomy) for benign cause between two methods of with and without prophylactic bilateral salpingectomy. Materials and Methods: In a prospective randomized clinical trial, 62 premenopausal women with benign indication underwent a laparoscopic hysterectomy. Patients were then randomized to undergo hysterectomy with bilateral salpingectomy (Group 1; n = 15) or without bilateral salpingectomy (Group 2; n = 15)...
April 2018: Journal of Cancer Research and Therapeutics
https://www.readbyqxmd.com/read/29892563/prevalence-of-hypovitaminosis-d-and-its-association-with-hypoadiponectinemia-and-hyperfollistatinemia-in-saudi-women-with-na%C3%A3-ve-polycystic-ovary-syndrome
#8
Osama Adnan Kensara
Aims: The association between vitamin D and polycystic ovary syndrome (PCOS) is an active area of growing research. However, data in Saudi Arabia are scarce. This study aimed to define serum 25-hydroxyvitamin D (25(OH)D) levels among Saudi women with naïve PCOS, and to investigate the associations of their 25(OH)D status with their serum adiponectin and follistatin levels, along with indices of insulin resistance and hormonal deteriorations. Methods: In this case-control observational study, 63 women with PCOS and 65 age-and body mass index (BMI)-matched control women were assessed...
June 2018: Journal of Clinical & Translational Endocrinology
https://www.readbyqxmd.com/read/29891752/systematic-analysis-of-long-non-coding-rnas-and-mrnas-in-the-ovaries-of-duroc-pigs-during-different-follicular-stages-using-rna-sequencing
#9
Yi Liu, Mengxun Li, Xinwen Bo, Tao Li, Lipeng Ma, Tenjiao Zhai, Tao Huang
The dynamic process involving the selection and maturation of follicles is regulated and controlled by a highly synchronized and exquisitely timed cascade of gene expression. Studies have shown that long non-coding RNA (lncRNA) is essential for the normal maintenance of animal reproductive function and has an important regulatory function in ovarian development and hormone secretion. In this study, a total of 2076 lncRNAs (1362 known lncRNAs and 714 new lncRNAs) and 25,491 mRNAs were identified in libraries constructed from Duroc ovaries on days 0, 2 and 4 of follicle development...
June 11, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29889977/ovulation-induction-in-polycystic-ovary-syndrome
#10
Tom Tanbo, Jan Mellembakken, Sverre Bjercke, Eva Ring, Thomas Åbyholm, Peter Fedorcsak
The objective of this narrative review is to suggest a rational order of treatment choices in anovulatory women with polycystic ovary syndrome (PCOS), for whom a multitude of treatment options exist. In obese/overweight PCOS patients the importance of weight reduction should be stressed. Inositol, a dietary supplement with a documented effect on ovulation and without side effects in the doses recommended, may be suggested. Additional first line medical alternatives include insulin sensitizers, selective estrogen receptor modulators and aromatase inhibitors...
June 11, 2018: Acta Obstetricia et Gynecologica Scandinavica
https://www.readbyqxmd.com/read/29887901/effect-of-chamomile-capsule-on-lipid-and-hormonal-related-parameters-among-women-of-reproductive-age-with-polycystic-ovary-syndrome
#11
Maryam Heidary, Zahra Yazdanpanahi, Mohammad Hossain Dabbaghmanesh, Mohammad Ebrahin Parsanezhad, Masoumeh Emamghoreishi, Marzieh Akbarzadeh
Background: According to traditional herbal medicine, chamomile has been considered as one of the herbal remedies for patients with polycystic ovary syndrome (PCOS). The study aimed to investigate the effect of chamomile on lipid and hormonal parameters in women of reproductive age with PCOS. Materials and Methods: This study is a randomized clinical trial which was conducted on 80 women (40 patients in each group) of childbearing age with PCO. The intervention group received 370 mg oral capsules of chamomile three times a day for 3 months...
2018: Journal of Research in Medical Sciences: the Official Journal of Isfahan University of Medical Sciences
https://www.readbyqxmd.com/read/29883426/a-functional-mutation-in-kiaa1462-promoter-decreases-glucocorticoid-receptor-affinity-and-affects-egg-laying-performance-in-yangzhou-geese
#12
Mengyuan Xia, Wei Wei, Zaohang Jiang, Dandan He, Zhen Li, Shigang Yu, Qiushi Wang, Honglin Liu, Jie Chen
The identification of genetic markers is valuable for improving the egg-laying performance in goose production. The single-nucleotide polymorphism (SNP) rs1714766362 in an intron of the goose KIAA1462 gene was found to be relevant to laying performance in our previous study. However, its function remains unclear. In this study, the full-length coding sequence of KIAA1462 gene was firstly characterized in Yangzhou geese. Q-PCR (Quantitative Real Time Polymerase Chain Reaction) results showed that KIAA1462 was highly expressed in the liver, ovary, and mature F1 follicles...
May 21, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29880879/reelin-and-aromatase-cooperate-in-ovarian-follicle-development
#13
Maurice Meseke, Felicitas Pröls, Camilla Schmahl, Katja Seebo, Claas Kruse, Nicola Brandt, Lars Fester, Lepu Zhou, Roland Bender, Gabriele M Rune
Reelin plays an important role in cerebral cortex development and synaptogenesis. In the hippocampus, the neurosteroid estrogen affects reelin expression. In this study we tested a potential crosstalk between estradiol and reelin, thus the possibility of a reelin-induced activation of the estradiol synthesizing enzyme aromatase. As a model system, we used ovaries, which express reelin and are a major source of estradiol. We found that in wild-type mice, reelin and aromatase are expressed in granulosa cells of growing follicles...
June 7, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29867282/changes-in-dna-methylation-of-oocytes-and-granulosa-cells-assessed-by-helmet-during-folliculogenesis-in-mouse-ovary
#14
Jin Liu, Wenchang Zhang, Zhiren Wu, Lei Dai, Takehiko Koji
For a better understanding of epigenetic regulation of cell differentiation, it is important to analyze DNA methylation at a specific site. In this study, we examined changes in the methylation level of CCGG and GATCG sites during mouse folliculogenesis in paraffin-embedded sections of mouse ovaries. For the purpose, we used a new method, histo endonuclease-linked detection of methylation sites of DNA (HELMET), designed to detect methylation sites of DNA with a specific sequence in a tissue section. Unlike the global level of DNA methylation, which was no change in immunohistochemical staining of 5-methylcytosine throughout folliculogenesis, we found that there were hypermethylation of CCGG and GATCG sites in most of the granulosa cells of tertiary follicles compared to that of primary and secondary follicles...
April 27, 2018: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/29863950/redescription-of-sciadocephalus-megalodiscus-diesing-1850-one-the-earliest-diverging-neotropical-fish-tapeworms-cestoda-proteocephalidae
#15
Tomáš Scholz, Alain de Chambrier
The tapeworm Sciadocephalus megalodiscus Diesing, 1850 (Cestoda: Proteocephalidae) is redescribed based on newly collected specimens parasitizing tucunare peacock bass, Cichla monoculus Agassiz, 1831 (Perciformes: Cichlidae), in the Peruvian Amazon. Even though this cestode was redescribed 2 decades ago by Rego et al. (1999), that redescription did not report some of the unique features of this species. The most unusual characteristics of the species are: (i) peculiar formation of the uterus, with fast formation of numerous, tightly packed diverticula protruding ventrally and dorsally, with simultaneous disintegration of the ovary and vitelline follicles in the first pregravid proglottids; (ii) inverted umbrella-shaped scolex with a well-developed apical sucker; (iii) a large-sized, follicular (grape cluster-like) ovary, which occupies most of the central (median) third of proglottids, with the ovarian isthmus situated almost equatorially; (iv) regular alternation of genital pores; (v) a well-developed internal seminal vesicle; and (vi) a small-sized strobila (shorter than 6 mm) consisting of few proglottids (15-20)...
June 4, 2018: Journal of Parasitology
https://www.readbyqxmd.com/read/29862276/the-protective-effects-of-glycyrrhetinic-acid-and-chrysin-against-ischemia-reperfusion-injury-in-rat-ovaries
#16
Rauf Melekoglu, Osman Ciftci, Sevil Eraslan, Saadet Alan, Nese Basak
Objective: To evaluate the protective effects of glycyrrhetinic acid (GA) and chrysin (CH) on experimental ischemia-reperfusion (I/R) injury in rat ovaries using tissue oxidative stress marker levels, hormone levels, and histopathological scores. Methods: Sixty healthy rats were randomly divided into six equal groups: control, I/R, I/R + CH (50 mg/kg/day), I/R + GA (100 mg/kg/day), CH (50 mg/kg/day), and GA (100 mg/kg/day). Biochemical, hormonal, and histopathological evaluations were performed on blood and tissue samples 14 days after CH and GA treatment...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29860415/follicle-dynamics-and-granulosa-cell-differentiation-in-the-turkey-hen-ovary
#17
Kahina Ghanem, A L Johnson
Similar to the domestic hen ovary, entry of a follicle into the preovulatory hierarchy in the turkey hen represents a process in which a single follicle initiates rapid growth and final maturation prior to ovulation. Published data derived from the laying hen support the proposal that differentiation of the follicle granulosa cell (GC) layer begins coincident with entry into the rapid growth phase and is characterized by the initial capacity for follicle stimulating hormone (FSH)-mediated cell signaling. The present studies were conducted with photostimulated B...
June 1, 2018: Poultry Science
https://www.readbyqxmd.com/read/29860321/premature-ovarian-insufficiency-and-early-depletion-of-the-ovarian-reserve-in-the-monogenic-mulibrey-nanism-disorder
#18
Susann Karlberg, Aila Tiitinen, Henrik Alfthan, Marita Lipsanen-Nyman
STUDY QUESTION: What is the timing of onset and clinical course of premature ovarian insufficiency (POI) in patients with Mulibrey nanism (MUL), a monogenic disorder caused by mutations of the peroxisomal TRIM37 gene? SUMMARY ANSWER: The number of ovarian follicles is highly reduced already in infant and young MUL girls and the majority of them will have early depletion of follicles resulting in clinical and biochemical signs of POI. WHAT IS KNOWN ALREADY: Both female and male patients with MUL show failure of sexual maturation, signs of hypogonadism and infertility...
June 1, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29857911/exercise-and-chemotherapy-induced-amenorrhea
#19
Katlynn M Mathis, Kathleen M Sturgeon, Renate M Winkels, Joachim Wiskemann, Nancy I Williams, Kathryn Schmitz
Chemotherapy-induced amenorrhea (CIA) is the temporary or permanent loss of menses experienced by premenopausal women undergoing chemotherapy treatment for cancer. Two possible mechanisms through which chemotherapy induces CIA have been identified: systemic endothelial dysfunction, resulting in decreased blood flow to the ovaries, and increased oxidative stress within the ovaries, both of which are proposed to lead to apoptosis of follicles. Endothelial dysfunction in ovarian arteries in women undergoing or who have undergone chemotherapy treatment is characterized by prothrombotic changes and thickening of the vascular wall...
July 2018: Medical Hypotheses
https://www.readbyqxmd.com/read/29851234/mitochondrial-unfolded-protein-response-gene-clpp-is-required-to-maintain-ovarian-follicular-reserve-during-aging-for-oocyte-competence-and-development-of-pre-implantation-embryos
#20
Tianren Wang, Elnur Babayev, Zongliang Jiang, Guangxin Li, Man Zhang, Ecem Esencan, Tamas Horvath, Emre Seli
Caseinolytic peptidase P mediates degradation of unfolded mitochondrial proteins and activates mitochondrial unfolded protein response (mtUPR) to maintain protein homeostasis. Clpp-/- female mice generate a lower number of mature oocytes and two-cell embryos, and no blastocysts. Clpp-/- oocytes have smaller mitochondria, with lower aspect ratio (length/width), and decreased expression of genes that promote fusion. A 4-fold increase in atretic follicles at 3 months, and reduced number of primordial follicles at 6-12 months are observed in Clpp-/- ovaries...
May 30, 2018: Aging Cell
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