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https://www.readbyqxmd.com/read/29231341/-effect-of-polycystic-ovary-syndrome-treated-with-the-periodic-therapy-of-acupuncture
#1
Danhong Yang, Mei Zhao, Jiahong Tan
OBJECTIVE: To observe the effects differences for polycystic ovary syndrome (PCOS) between periodic therapy of acupuncture and diane-35 and to explore its mechanism. METHODS: Sixty patients were randomly assigned into an acupuncture group and a western medication group, 30 cases in each one, 29 patients finally included with one case dropping out respectively. In the acupuncture group, periodic therapy was mainly used at bilateral Sanyinjiao (SP 6), Dahe (KI 12), Guilai (ST 29), Guanyuan (CV 4), Zhongji (CV 3), with different acupoints in the postmenstrual, intermenstrual, premenstrual periods, once every other day, 3 times a week...
August 12, 2017: Zhongguo Zhen Jiu, Chinese Acupuncture & Moxibustion
https://www.readbyqxmd.com/read/29230615/association-of-604g-a-and-501a-c-ghrelin-and-obestatin-prepropeptide-gene-polymorphisms-with-polycystic-ovary-syndrome
#2
Talaat Dabbaghi Ghaleh, Somayeh Saadat Skandari, Reza Najafipour, Zahra Rashvand, Masoud Darabi, Mehdi Sahmani
Ghrelin hormone has an important role in a wide range of metabolic and non-metabolic processes. Polymorphisms of ghrelin gene could be associated with a large number of diseases. The aim of this study was to evaluate the association of -604G/A and -501A/C polymorphisms in ghrelin and obestatin prepropeptide gene (GHRL) with polycystic ovary syndrome (PCOS) in a sample of Iranian women. One hundred and fifty-two women with PCOS and 162 age-matched apparently healthy women as control group were enrolled in this study...
December 11, 2017: Biochemical Genetics
https://www.readbyqxmd.com/read/29230582/a-new-monozoic-tapeworm-parabreviscolex-niepini-n-g-n-sp-cestoda-caryophyllidea-from-schizothoracine-fishes-cyprinidae-schizothoracinae-in-tibet-china
#3
Bing-Wen Xi, Mikuláš Oros, Kai Chen, Jun Xie
A new monozoic cestode, Parabreviscolex niepini n. gen. and n. sp. (Cestoda: Caryophyllidea), is described from the type-host Schizopygopsis younghusbandi Regan, 1905 (Cyprinidae: Schizothoracinae) and Schizothorax waltoni Regan, 1905 (Cyprinidae: Schizothoracinae) in the Yarlung Tsangpo River, the upper tributary of the Brahmaputra River on the Tibetan Plateau. The new genus is placed in the Capingentidae because the vitellarium is situated partly in the medullary and cortical parenchyma, i.e., neither completely external nor internal to inner longitudinal muscles...
December 11, 2017: Parasitology Research
https://www.readbyqxmd.com/read/29229882/igf1-stimulates-differentiation-of-primary-follicles-and-their-growth-in-ovarian-explants-of-zebrafish-danio-rerio-cultured-in-vitro
#4
Pancharatna A Katti, Sheetal S Narvekar, Basavaraj B Goundadkar, Prasad A Deshpande
The present study is an attempt to elucidate the involvement of insulin-like growth factor (IGF1) in the differentiation and growth of primary follicles in ovarian explant cultures of zebrafish. Ovaries from adult females were cultured in triplicate sets/treatment group for 15 days at 22° in the laboratory. Culture medium was supplemented with either insulin (1 ng/mL) or IGF1 (1 ng/mL) or insulin + IGF1 (Experiment 1) or 0.1 or 1.0 or 10 ng/mL of IGF1 (Experiment 2). Ovaries cultured in medium alone served as controls and those fixed at the beginning of the culture as initial controls...
December 2017: Journal of Biosciences
https://www.readbyqxmd.com/read/29228146/identification-and-characterization-of-a-specific-13-mirna-expression-signature-during-follicle-activation-in-the-zebrafish-ovary
#5
Queenie Wing-Lei Wong, Ming-An Sun, Shuk-Wa Lau, Chirag Parsania, Shaolong Zhou, Silin Zhong, Wei Ge
Ovarian folliculogenesis is always of great interest in reproductive biology. However, the molecular mechanisms that control follicle development, particularly the early phase of follicle activation or recruitment, still remain poorly understood. In an attempt to decipher the gene networks and signaling pathways involved in such transition, we conducted a transcriptomic analysis (RNA-seq) on zebrafish primary growth (PG, stage I; inactive) and pre-vitellogenic (PV, stage II; activated) follicles. A total of 118 unique microRNAs (miRNAs) (11 down-regulated and 83 up-regulated during PG/PV transition) and 56711 unique messenger RNAs (mRNAs) (1839 down-regulated and 7243 up-regulated during PG/PV transition) were identified...
December 6, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29228129/prenatal-exposure-to-di-2-ethylhexyl-phthalate-causes-long-term-transgenerational-effects-on-female-reproduction-in-mice
#6
Emily Brehm, Saniya Rattan, Liying Gao, Jodi A Flaws
Di(2-ethylhexyl) phthalate (DEHP) is a plasticizer in many consumer products. Although DEHP is a known endocrine disruptor, little is known about the effects of DEHP exposure on female reproduction. Thus, this study tested the hypothesis that prenatal DEHP exposure affects follicle numbers, estrous cyclicity, and hormone levels in multiple generations of mice. Pregnant CD-1 mice were orally dosed with corn oil (vehicle control) or DEHP (20 and 200 µg/kg/day, and 500 and 750 mg/kg/day) from gestation day 10...
December 7, 2017: Endocrinology
https://www.readbyqxmd.com/read/29224376/regulation-of-follicle-growth-through-hormonal-factors-and-mechanical-cues-mediated-by-hippo-signaling-pathway
#7
Ikko Kawashima, Kazuhiro Kawamura
The ovary is an interesting organ that shows major structural changes within a short period of time during each reproductive cycle. Follicle development is controlled by local paracrine and systemic endocrine factors. Many hormonal and molecular analyses have been conducted to find the mechanisms underlying structural changes in ovaries, However, exact mechanisms still remain to be determined. Recent development of mechanobiology facilitates the understanding on the contribution of physical forces and changes in the mechanical properties of cells and tissues to physiology and pathophysiology...
December 9, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29222841/evaluation-of-an-ovary-on-a-chip-in-large-mammalian-models-species-specificity-and-influence-of-follicle-isolation-status
#8
J Nagashima, R El Assal, N Songsasen, U Demirci
The ability to grow oocytes from immature ovarian follicles in vitro has significant potential for fertility preservation; yet, it has proved challenging in large mammalian species due to the complex metabolic needs and long-term culture requirements. Currently, follicular incubations are based on a 'static' system with manual exchange of medium. Despite the numerous advantages of conventional culturing approaches, recapitulating the native microenvironment and supporting the survival of ovarian follicles still represent challenges...
December 8, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29221447/disruption-of-postnatal-folliculogenesis-and-development-of-ovarian-tumor-in-a-mouse-model-with-aberrant-transforming-growth-factor-beta-signaling
#9
Yang Gao, Xin Fang, David F Vincent, David W Threadgill, Laurent Bartholin, Qinglei Li
BACKGROUND: Transforming growth factor beta (TGFB) superfamily signaling is implicated in the development of sex cord-stromal tumors, a category of poorly defined gonadal tumors. The aim of this study was to determine potential effects of dysregulated TGFB signaling in the ovary using Cre recombinase driven by growth differentiation factor 9 (Gdf9) promoter known to be expressed in oocytes. METHODS: A mouse model containing constitutively active TGFBR1 (TGFBR1CA) using Gdf9-iCre (termed TGFBR1-CAG9Cre) was generated...
December 8, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/29221424/effects-of-dehydroepiandrosterone-supplementation-on-mice-with-diminished-ovarian-reserve
#10
Xiufeng Lin, Jing Du, Yan Du, Riran Wu, Xiaowu Fang, Yuechan Liao, Song Quan
Dehydroepiandrosterone (DHEA) has been used to improve the pregnancy rate in women with diminished ovarian reserve(DOR) during in vitro fertilization. We aimed to validate the effects of DHEA and identify the possible mechanisms. We constructed a mice model with DOR and analyzed the hormone parameters and follicle counts. In vivo experiment, FSH and LH concentrations in the serum were significantly elevated in the DOR group. However, the FSH and LH concentrations were partially reversed in the DOR + DHEA group...
December 8, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/29217212/ovarian-morphology-by-transabdominal-ultrasound-correlates-with-reproductive-and-metabolic-disturbance-in-adolescents-with-pcos
#11
Beth W Rackow, Heidi Vanden Brink, Lynwood Hammers, Clare A Flannery, Marla E Lujan, Tania S Burgert
PURPOSE: To determine whether ovarian morphology imaged using transabdominal ultrasonography reflects clinical and metabolic features in adolescents with polycystic ovary syndrome (PCOS). METHODS: A retrospective pilot study was conducted in 33 adolescents (12-18 years) with PCOS as defined by hyperandrogenism and irregular cycles. Adolescents underwent the following assessments at a random time during the menstrual cycle: transabdominal ultrasonography, physical examination (height, weight, and systolic and diastolic blood pressure), fasting hormonal tests (free, percent free, and total testosterone, androstenedione, follicle stimulating hormone, luteinizing hormone), and metabolic tests (including an oral glucose tolerance test, fasting and 2-hour insulin and glucose, homeostatic model assessment of insulin resistance, and whole-body insulin sensitivity index)...
December 5, 2017: Journal of Adolescent Health: Official Publication of the Society for Adolescent Medicine
https://www.readbyqxmd.com/read/29217167/potential-protective-effect-of-pistacia-lentiscus-oil-against-chlorpyrifos-induced-hormonal-changes-and-oxidative-damage-in-ovaries-and-thyroid-of-female-rats
#12
Samira Chebab, Fatiha Mekircha, Essaid Leghouchi
The purpose of this study was to evaluate the protective effect of Pistacia lentiscus oil (PLO), known for its antioxidant properties, on chlorpyrifos (CPF)-induced alterations in the thyroid, reproductive hormone levels, and oxidative damage in the ovaries and thyroid of adult Wistar rats. The animals were treated with orally administered PLO (2 mL/kg), CPF (6.75 mg/kg), and a combination of CPF and PLO for 30 days. Serum levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), progesterone (Pg), estradiol (E2), triiodothyronine (T3), thyroxine (T4), and thyroid-stimulating hormone (TSH) were assessed using chemiluminescence assay...
December 4, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29215516/clomiphene-stair-step-protocol-for-women-with-polycystic-ovary-syndrome
#13
Tiffanny Jones, Jacqueline R Ho, Marc Gualtieri, Janet Bruno-Gaston, Karine Chung, Richard J Paulson, Kristin A Bendikson
OBJECTIVE: To compare time to ovulation, ovulation rates, and side effect profile of traditional and the stair-step protocol for ovulation induction using clomiphene citrate in women with polycystic ovary syndrome (PCOS). METHODS: We performed a retrospective study of women seeking care for infertility with a diagnosis of PCOS at a university-based infertility clinic from July 2012 to July 2014. We included patients who were resistant to the initial starting dose of 50 mg clomiphene...
December 4, 2017: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/29214681/thyroid-hormones-alter-estrous-cyclicity-and-antioxidative-status-in-the-ovaries-of-rats
#14
Quanwei Wei, Jaafar Sulieman Fedail, Lingfa Kong, Kaizhi Zheng, Chunhua Meng, Mohamed Babo Fadlalla, Fangxiong Shi
To expand our understanding of the roles of thyroid hormones on female reproduction, we induced hypo- and hyper-T rat models to investigate the roles of thyroid hormones on estrous cyclicity, as well as the antioxidative status in the ovaries of rats. In the current study, our data show that hypothyroidism (hypo-T) and hyperthyroidism (hyper-T) led to significantly reduced body weights and ovarain weights and delayed vaginal opening day. For hyper-T, thyroxine (T4), tri-iodothyronine (T3), progesterone (P4) and follicle-stimulating hormone (FSH) were significantly increased, while estradiol (E2) and luteinizing hormone (LH) were significantly decreased...
December 7, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/29207291/transcriptome-analysis-of-ovine-granulosa-cells-reveals-differences-between-small-antral-follicles-collected-during-the-follicular-and-luteal-phases
#15
Reza Talebi, Ahmad Ahmadi, Fazlollah Afraz, Julien Sarry, Florence Plisson-Petit, Carine Genêt, Stéphane Fabre
Ovarian follicular growth occurs in both the follicular and luteal phases of the estrous cycle but in very different endocrine contexts. In both phases, many small antral follicles with similar morphologic and histologic characteristics are present within the ovaries as a reserve for the terminal folliculogenesis. However, there are several gaps in our molecular knowledge of the gene expression profiles of small antral follicles in the follicular and luteal phases. The aim of the present study was to use RNA sequencing to compare and analyze the global transcriptional profile of ovine granulosa cells collected from small antral follicles (1-3 mm) either during the follicular or the luteal phase of the estrous cycle, with the hypothesis that they should be differential...
November 27, 2017: Theriogenology
https://www.readbyqxmd.com/read/29207252/role-of-activin-c-in-normal-ovaries-and-granulosa-cell-tumours-of-mice-and-humans
#16
Karen L Reader, Francesco E Marino, Helen D Nicholson, Gail P Risbridger, Elspeth J Gold
Activins and inhibins play important roles in the development, growth and function of the ovary. Mice lacking inhibin develop granulosa cell tumours in their ovaries that secrete activin A, and these tumours are modulated by increased activin C expression. The aim of the present study was to identify where activin C is expressed in mouse and human ovaries and whether overexpression of activin C modulates normal follicular development in mice. Immunohistochemical staining for the activin βC subunit was performed on sections from mouse and human ovaries and human adult granulosa cell tumours...
December 6, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29206944/the-direct-and-indirect-effects-of-kisspeptin-54-on-granulosa-lutein-cell-function
#17
L A Owens, A Abbara, A Lerner, S O'floinn, G Christopoulos, S Khanjani, R Islam, K Hardy, A C Hanyaloglu, S A Lavery, W S Dhillo, S Franks
STUDY QUESTION: What are the in vivo and in vitro actions of kisspeptin-54 on the expression of genes involved in ovarian reproductive function, steroidogenesis and ovarian hyperstimulation syndrome (OHSS) in granulosa lutein (GL) cells when compared with traditional triggers of oocyte maturation? SUMMARY ANSWER: The use of kisspeptin-54 as an oocyte maturation trigger augmented expression of genes involved in ovarian steroidogenesis in human GL cells including, FSH receptor (FSHR), LH/hCG receptor (LHCGR), steroid acute regulatory protein (STAR), aromatase, estrogen receptors alpha and beta (ESR1, ESR2), 3-beta-hydroxysteroid dehydrogenase type 2 (3BHSD2) and inhibin A (INHBA), when compared to traditional maturation triggers, but did not alter markers of OHSS...
December 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29205453/localization-of-thrombospondin-1-and-its-receptor-cd36-in-the-ovary-of-the-ostrich-struthio-camelus
#18
D Rodler, F Sinowatz
Angiogenesis, the formation of new blood vessels from pre-existing vasculature, plays a decisive role for the rapid growth of avian follicles. Compared to mammals, few data on the angiogenesis in the avian ovary are available. However, whereas several pro-angiogenic factors in the avian ovary have been recently studied in detail, little information is available on the localization of anti-angiogenic factors. The aim of this study was to determine the localization and possible function of the anti-angiogenic factor thrombospondin-1 (TSP-1) and its receptor CD36 in the ovary of the ostrich using immunohistochemistry and to correlate the results with ultrastructural data...
December 4, 2017: Anatomia, Histologia, Embryologia
https://www.readbyqxmd.com/read/29201665/the-effect-of-curcumin-on-tnf-%C3%AE-il-6-and-crp-expression-in-a-model-of-polycystic-ovary-syndrome-as-an-inflammation-state
#19
Shima Mohammadi, Parvin Kayedpoor, Latifeh Karimzadeh-Bardei, Mohammad Nabiuni
Background: Having low-grade chronic inflammation such as elevated C-reactive protein (CRP), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) plays a crucial role in polycystic ovary syndrome (PCOS). This study aimed at investigating the therapeutic effects of curcumin on IL-6, CRP and TNF-α and symptoms of polycystic ovary syndrome. Methods: In this research, 72 female adult Wistar rats were divided into control (n=12), PCOS (n=12) and curcumin-treated PCOS groups (n=48)...
October 2017: Journal of Reproduction & Infertility
https://www.readbyqxmd.com/read/29196492/high-fat-diet-exposure-from-pre-pubertal-age-induces-pcos-in-rats
#20
Roshni S Patel, Gaurang B Shah
Polycystic ovary syndrome (PCOS) is associated with hyperandrogenism, oligo-anovulation, polycystic ovaries and metabolic syndrome. Many researchers reported that PCOS often starts with menarche in adolescents. Presently available animal models focuses on ovarian but not metabolic features of PCOS. Therefore, we hypothesized that high fat diet feeding to pre-pubertal female rats results in both reproductive and metabolic features of PCOS. Pre-pubertal female rats were divided into two groups: group I received normal pellet diet and group II received high fat diet (HFD)...
December 1, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
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