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https://www.readbyqxmd.com/read/28533816/conferring-cellulose-degrading-ability-to-yarrowia-lipolytica-to-facilitate-a-consolidated-bioprocessing-approach
#1
Zhong-Peng Guo, Sophie Duquesne, Sophie Bozonnet, Gianluca Cioci, Jean-Marc Nicaud, Alain Marty, Michael Joseph O'Donohue
BACKGROUND: Yarrowia lipolytica, one of the most widely studied "nonconventional" oleaginous yeast species, is unable to grow on cellulose. Recently, we identified and overexpressed two endogenous β-glucosidases in Y. lipolytica, thus enabling this yeast to use cello-oligosaccharides as a carbon source for growth. Using this engineered yeast platform, we have now gone further toward building a fully cellulolytic Y. lipolytica for use in consolidated bioprocessing of cellulose. RESULTS: Initially, different essential enzyme components of a cellulase cocktail (i...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28528384/effects-of-salts-contained-in-lignocellulose-derived-sugar-streams-on-microbial-lipid-production
#2
Zhu Chen, Caixia Wan
This study aimed at developing low-cost, robust non-sterile fermentation processes for microbial lipid production from lignocellulose-derived sugars. Three representative oleaginous yeasts, Lipomyces tetrasporus (NRRL Y-11562), Rhodotorula toruloides (NRRL Y-1091), and Yarrowia lipolytica (NRRL YB-437), were tested for lipid production via non-sterile fermentation. Under optimal non-sterile conditions, all the tested strains had good performance on salt tolerance and lipid production. L. tetrasporus (NRRL Y-11562) gave the highest lipid titer of 12...
May 20, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28505129/assessment-of-antimicrobial-and-antioxidant-activities-of-nepeta-trachonitica-analysis-of-its-phenolic-compounds-using-hplc-ms-ms
#3
Ekrem Köksal, Hatice Tohma, Ömer Kılıç, Yusuf Alan, Abdülmelik Aras, İlhami Gülçin, Ercan Bursal
Continuing our work on the sources of natural bioactive compounds, we evaluated the antimicrobial and antioxidant activities of Nepeta trachonitica as well as its major phenolic content using the high-performance liquid chromatography-mass spectrometry/mass spectrometry (HPLC-MS/MS) technique. For antioxidant activity, ferric reducing antioxidant power (FRAP) and cupric ion reducing antioxidant capacity (CUPRAC) methods were performed to measure the reducing power and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay was employed to evaluate the radical scavenging activity of the sample...
May 15, 2017: Scientia Pharmaceutica
https://www.readbyqxmd.com/read/28497289/systematic-analysis-of-the-lysine-acetylome-reveals-diverse-functions-of-lysine-acetylation-in-the-oleaginous-yeast-yarrowia-lipolytica
#4
Guangyuan Wang, Lizhong Guo, Wenxing Liang, Zhenming Chi, Lin Liu
Lysine acetylation of proteins, a major post-translational modification, plays a critical regulatory role in almost every aspects in both eukaryotes and prokaryotes. Yarrowia lipolytica, an oleaginous yeast, is considered as a model for bio-oil production due to its ability to accumulate a large amount of lipids. However, the function of lysine acetylation in this organism is elusive. Here, we performed a global acetylproteome analysis of Y. lipolytica ACA-DC 50109. In total, 3163 lysine acetylation sites were identified in 1428 proteins, which account for 22...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28485370/the-digestive-and-defensive-basis-of-carcass-utilization-by-the-burying-beetle-and-its-microbiota
#5
Heiko Vogel, Shantanu P Shukla, Tobias Engl, Benjamin Weiss, Rainer Fischer, Sandra Steiger, David G Heckel, Martin Kaltenpoth, Andreas Vilcinskas
Insects that use ephemeral resources must rapidly digest nutrients and simultaneously protect them from competitors. Here we use burying beetles (Nicrophorus vespilloides), which feed their offspring on vertebrate carrion, to investigate the digestive and defensive basis of carrion utilization. We characterize gene expression and microbiota composition in the gut, anal secretions, and on carcasses used by the beetles. We find a strict functional compartmentalization of the gut involving differential expression of immune effectors (antimicrobial peptides and lysozymes), as well as digestive and detoxifying enzymes...
May 9, 2017: Nature Communications
https://www.readbyqxmd.com/read/28483524/%C3%AE-12-fatty-acid-desaturase-is-involved-in-growth-at-low-temperature-in-yeast-yarrowia-lipolytica
#6
Satoshi Tezaki, Ryo Iwama, Satoshi Kobayashi, Yuh Shiwa, Hirofumi Yoshikawa, Akinori Ohta, Hiroyuki Horiuchi, Ryouichi Fukuda
We investigated the role of FAD2, which was predicted to encode a fatty acid desaturase of the n-alkane-assimilating yeast Yarrowia lipolytica. Northern blot analysis suggested that FAD2 transcription was upregulated at low temperature or in the presence of n-alkanes or oleic acid. The FAD2 deletion mutant grew as well as the wild-type strain on glucose, n-alkanes, or oleic acid at 30 °C, but grew at a slower rate at 12 °C, when compared to the wild-type strain. The growth of the FAD2 deletion mutant at 12 °C was restored by the addition of 18:2, but not 18:1, fatty acids...
June 17, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28482902/substrates-and-oxygen-dependent-citric-acid-production-by-yarrowia-lipolytica-insights-through-transcriptome-and-fluxome-analyses
#7
Wael Sabra, Rajesh Reddy Bommareddy, Garima Maheshwari, Seraphim Papanikolaou, An-Ping Zeng
BACKGROUND: Unlike the well-studied backer yeast where catabolite repression represents a burden for mixed substrate fermentation, Yarrowia lipolytica, an oleaginous yeast, is recognized for its potential to produce single cell oils and citric acid from different feedstocks. These versatilities of Y. lipolytica with regards to substrate utilization make it an attractive host for biorefinery application. However, to develop a commercial process for the production of citric acid by Y. lipolytica, it is necessary to better understand the primary metabolism and its regulation, especially for growth on mixed substrate...
May 8, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28479605/effect-of-oils-extracted-from-plant-seeds-on-the-growth-and-lipolytic-activity-of-yarrowia-lipolytica-yeast
#8
Jolanta Krzyczkowska, Mariola Kozłowska
This study was aimed at evaluating the capability of Yarrowia lipolytica W29 for the synthesis of lipolytic enzymes in a medium containing plant oils from non-conventional sources with some components displaying bioactivity. Oils from almond, hazelnut, and coriander seeds were obtained by using n-hexane (Soxhlet method) and a chloroform/methanol mixture of solvents (Folch method), and their effect on the growth and lipolytic activity of Y. lipolytica was compared. A comparison of these two extraction methods showed that the extraction with n-hexane was less effective regarding the oil extraction yields than the extraction conducted according to Folch's procedure...
2017: Journal of the American Oil Chemists' Society
https://www.readbyqxmd.com/read/28475761/sugar-versus-fat-elimination-of-glycogen-storage-improves-lipid-accumulation-in-yarrowia-lipolytica
#9
Govindprasad Bhutada, Martin Kavšcek, Rodrigo Ledesma-Amaro, Stéphane Thomas, Gerald N Rechberger, Jean-Marc Nicaud, Klaus Natter
Triacylglycerol (TAG) and glycogen are the two major metabolites for carbon storage in most eukaryotic organisms. We investigated the glycogen metabolism of the oleaginous Yarrowia lipolytica and found that this yeast accumulates up to 16% glycogen in its biomass. Assuming that elimination of glycogen synthesis would result in an improvement of lipid accumulation, we characterized and deleted the single gene coding for glycogen synthase, YlGSY1. The mutant was grown under lipogenic conditions with glucose and glycerol as substrates and we obtained up to 60% improvement in TAG accumulation compared to the wild-type strain...
May 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28474529/restoring-of-glucose-metabolism-of-engineered-yarrowia-lipolytica-for-succinic-acid-production-via-a-simple-and-efficient-adaptive-evolution-strategy
#10
Xiaofeng Yang, Huaimin Wang, Chong Li, Carol Sze Ki Lin
Succinate dehydrogenase inactivation in Yarrowia lipolytica has been demonstrated for robust succinic acid production, whereas the inefficient glucose metabolism has hindered its practical application. In this study, a simple and efficient adaptive evolution strategy via cell immobilization was conducted in shake flasks, with an aim to restore the glucose metabolism of Y. lipolytica mutant PGC01003. After 21 days with 14 generations evolution, glucose consumption rate increased to 0.30 g/L/h in YPD medium consisting of 150 g/L initial glucose concentration, while poor yeast growth was observed in the same medium using the initial strain without adaptive evolution...
May 12, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28465213/metabolomic-changes-and-metabolic-responses-to-expression-of-heterologous-biosynthetic-genes-for-lycopene-production-in-yarrowia-lipolytica
#11
Chen Zhao, Qi Gao, Jun Chen, Liujing Wei, Tadayuki Imanaka, Qiang Hua
The metabolomic studies were conducted for both the engineered lycopene-producing Yarrowia lipolytica strain and the control strain. The changes in metabolome profiling and main pathway abundance of Y. lipolytica with the introduction of heterologous crt genes were investigated by multiple analytical and statistical methods More importantly, the metabolome data were transformed into pathway abundances visualized by a pathway relationship network using Cytoscape software. This network provided a simple way to present the statistical results of pathway abundance and interpret the effects of the crt genes in a metabolic view, and the results highlighted the up-regulation of eight pathways and the down-regulation of three pathways in the lycopene fast production phase...
April 29, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28443314/cutaneous-yarrowia-lipolytica-infection-in-an-immunocompetent-woman
#12
Alan S Boyd, Lee Wheless, Bobbi G Brady, Darrel Ellis
No abstract text is available yet for this article.
May 2017: JAAD Case Reports
https://www.readbyqxmd.com/read/28419226/sugar-versus-fat-elimination-of-glycogen-storage-improves-lipid-accumulation-in-yarrowia-lipolytica
#13
Govindprasad Bhutada, Martin Kavšcek, Rodrigo Ledesma-Amaro, Stéphane Thomas, Gerald N Rechberger, Jean-Marc Nicaud, Klaus Natter
Triacylglycerol (TAG) and glycogen are the two major metabolites for carbon storage in most eukaryotic organisms. We investigated the glycogen metabolism of the oleaginous Y. lipolytica and found that this yeast accumulates up to 16% glycogen in its biomass. Assuming that elimination of glycogen synthesis would result in an improvement of lipid accumulation, we characterized and deleted the single gene coding for glycogen synthase, YlGSY1. The mutant was grown under lipogenic conditions with glucose and glycerol as substrates and we obtained up to 60% improvement in TAG accumulation compared to the wild type strain...
April 17, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28414174/iterative-integration-of-multiple-copy-pathway-genes-in-yarrowia-lipolytica-for-heterologous-%C3%AE-carotene-production
#14
Shuliang Gao, Yangyang Tong, Li Zhu, Mei Ge, Yian Zhang, Daijie Chen, Yu Jiang, Sheng Yang
β-Carotene is a terpenoid molecule with high hydrophobicity that is often used as an additive in foods and feed. Previous work has demonstrated the heterologous biosynthesis of β-carotene from an intrinsic high flux of acetyl-CoA in 12 steps through 11 genes in Yarrowia lipolytica. Here, an efficient biosynthetic pathway capable of producing 100-fold more β-carotene than the baseline construct was generated using strong promoters and multiple gene copies for each of the 12 steps. Using fed-batch fermentation with an optimized medium, the engineered pathway could produce 4g/L β-carotene, which was stored in lipid droplets within engineered Y...
April 13, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28398122/investigating-proteome-and-transcriptome-defense-response-of-apples-induced-by-yarrowia-lipolytica
#15
Hongyin Zhang, Liangliang Chen, Yiwen Sun, Lina Zhao, Xiangfeng Zheng, Qiya Yang, Xiaoyun Zhang
A better understanding of the mode of action of postharvest biocontrol agents on fruit surfaces is critical for the advancement of successful implementation of postharvest biocontrol products. This is due to the increasing importance of biological control of postharvest diseases over chemical and other control methods. However, most of the mechanisms involved in biological control remain unknown and need to be explored. Yarrowia lipolytica significantly inhibited blue mold decay of apples caused by Penicillium expansum...
April 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28388696/the-use-of-serine-protease-from-yarrowia-lipolytica-yeast-in-the-production-of-biopeptides-from-denatured-egg-white-proteins
#16
Marta Pokora, Aleksandra Zambrowicz, Agnieszka Zabłocka, Anna Dąbrowska, Marek Szołtysik, Konrad Babij, Ewelina Eckert, Tadeusz Trziszka, Józefa Chrzanowska
Deriving non-conventional enzymes from cheaper sources than those used for commercially available enzymes may result in the production of hydrolysates with beneficial features, while drastically reducing the cost of hydrolysis. This is especially significant for enzymatic hydrolysis as a method of protein waste utilization. We have previously described the ability of non-commercial serine protease from Yarrowia lipolytica yeast to produce/release bioactive peptides from egg white protein by-products (EP). The enzymatic hydrolysis of EP was carried out for 24 h using the serine protease at an enzyme: substrate ratio of 1:30 (w/w)...
April 7, 2017: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/28384620/recovering-traditional-raw-milk-tetilla-cheese-flavour-and-sensory-attributes-by-using-kocuria-varians-and-yarrowia-lipolytica-adjunct-cultures
#17
J A Centeno, J I Garabal, F Docampo, J M Lorenzo, J Carballo
The rationale of the present study was to evaluate the potential of microbial adjunct cultures including Kocuria varians and/or Yarrowia lipolytica strains in the recovery of the typical sensory profile of traditional (raw-milk) Tetilla cheese. Four batches of Tetilla cheese, a short ripened cows' milk cheese produced in Galicia (NW Spain), were made in duplicate from pasteurized milk inoculated with different microbial cultures. A control batch was manufactured by adding a mesophilic commercial D-starter only...
March 22, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28376264/synthesis-and-structure-activity-relationships-of-novel-benzylamine-type-antifungals-as-butenafine-related-antimycotics
#18
Jürgen Krauss, Martina Stadler, Franz Bracher
Benzylamine-type antimycotics like naftifine, butenafine, or terbinafine are a well-known class of antimycotics since the 1980s. The following paper describes the synthesis and biological evaluation of a series of novel benzylamine-type antimycotics characterized by an isooctyl side chain and various substituents at the benzylamine moiety. The compounds were prepared from benzaldehyde derivatives and 2-amino-6-methylheptane by reductive amination with sodium triacetoxyborohydride and subsequent precipitation with hydrogen chloride...
April 4, 2017: Archiv der Pharmazie
https://www.readbyqxmd.com/read/28357621/improved-campesterol-production-in-engineered-yarrowia-lipolytica-strains
#19
Yu Zhang, Ying Wang, Mingdong Yao, Hong Liu, Xiao Zhou, Wenhai Xiao, Yingjin Yuan
OBJECTIVES: To engineer Yarrowia lipolytica for improving the heterologous production of campesterol (a key precursor to manufacture pharmaceutical steroids). RESULTS: By screening 7-dehydrocholesterol reductase (DHCR7) from diverse species, DHCR7 from Danio rerio was the best candidate for campesterol synthesis. Overexpression of ACL (ATP: citrate lyase) or POX2 (peroxisome acyl-CoA oxidase 2) were key to improving campesterol production. The highest yield of campesterol was 942 mg/l was with the strain overexpressing POX2 in a 5 l bioreactor via high cell density fermentation process with a restricted supply of carbon sourc, sunflower seed oil...
March 29, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28357546/a-metabolic-engineering-strategy-for-producing-conjugated-linoleic-acids-using-the-oleaginous-yeast-yarrowia-lipolytica
#20
Nabila Imatoukene, Jonathan Verbeke, Athanasios Beopoulos, Abdelghani Idrissi Taghki, Brigitte Thomasset, Claude-Olivier Sarde, Maurice Nonus, Jean-Marc Nicaud
Conjugated linoleic acids (CLAs) have been found to have beneficial effects on human health when used as dietary supplements. However, their availability is limited because pure, chemistry-based production is expensive, and biology-based fermentation methods can only create small quantities. In an effort to enhance microbial production of CLAs, four genetically modified strains of the oleaginous yeast Yarrowia lipolytica were generated. These mutants presented various genetic modifications, including the elimination of β-oxidation (pox1-6∆), the inability to store lipids as triglycerides (dga1∆ dga2∆ are1∆ lro1∆), and the overexpression of the Y...
March 29, 2017: Applied Microbiology and Biotechnology
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