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https://www.readbyqxmd.com/read/28731568/a-strong-hybrid-fatty-acid-inducible-transcriptional-sensor-built-from-yarrowia-lipolytica-upstream-activating-and-regulatory-sequences
#1
Murtaza Shabbir-Hussain, Ian Wheeldon, Mark A Blenner
The engineering of Yarrowia lipolytica to accumulate lipids with high titers and productivities has been enabled with a handful of constitutive promoters for pathway engineering. However, the development of promoters that are both strong and lipid responsive could greatly benefit the bioproduction efficiency of lipid-derived oleochemicals in oleaginous yeast. in this study, we sought to engineer a fatty acid regulated hybrid promoter for use in Y. lipolytica. We identified a 200 bp upstream regulatory sequence in the peroxisomal acyl CoA oxidase 2 (POX2) promoter...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28714266/potential-biotechnological-capabilities-of-cultivable-mycobiota-from-carwash-effluents
#2
Timothy Sibanda, Ramganesh Selvarajan, Memory Tekere, Hlengilizwe Nyoni, Stephen Meddows-Taylor
Urban life has created man-made extreme environments like carwashes. These environments have, however, not been sufficiently explored for mycobiota that can be sources of biotechnologically useful products, as has been the case with natural extreme environments. Using a combination of culture and molecular techniques, fungi from carwash effluents was characterized for production of lipase and cellulase enzymes, nonpolar and polar biotechnologically relevant secondary metabolites and hydrocarbon utilization...
July 17, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28704940/effects-of-protein-iron-complex-concentrate-supplementation-on-iron-metabolism-oxidative-and-immune-status-in-preweaning-calves
#3
Robert Kupczyński, Michał Bednarski, Kinga Śpitalniak, Krystyna Pogoda-Sewerniak
The objective of this study was to determine the effects of feeding protein-iron complex (PIC) on productive performance and indicators of iron metabolism, hematology parameters, antioxidant and immune status during first 35 days of a calf's life. Preparation of the complex involved enzymatic hydrolysis of milk casein (serine protease from Yarrowia lipolytica yeast). Iron chloride was then added to the hydrolyzate and lyophilizate. Calves were divided into treated groups: LFe (low iron dose) 10 g/day calf of protein-iron complex, HFe (height iron dose) 20 g/day calf, and control group...
July 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28702791/erratum-to-identification-and-characterization-of-eyk1-a-key-gene-for-erythritol-catabolism-in-yarrowia-lipolytica
#4
F Carly, H Gamboa-Melendez, M Vandermies, C Damblon, J M Nicaud, P Fickers
No abstract text is available yet for this article.
July 12, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28694185/enhanced-production-of-nisin-by-co-culture-of-lactococcus-lactis-sub-sp-lactis-and-yarrowia-lipolytica-in-molasses-based-medium
#5
Mehdi Ariana, Javad Hamedi
Nisin is a safe, approved and commercial bacteriocin that is produced by Lactococcus lactis subsp. lactis. Since lactate accumulation in fermentation medium reduces L. lactis growth and nisin production, Yarrowia lipolytica, a lactate consuming yeast and L. lactis subsp. lactis, were simultaneously cultured in a molasses based medium. Y. lipolytica is not able to consume sucrose as carbon source, but rather consumes lactate and hence decrease lactic acid titer by 10% in the medium. Lactic acid consumption, 15% increased pH value and stimulated L...
July 8, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28693571/characterization-of-erythrose-reductase-from-yarrowia-lipolytica-and-its-influence-on-erythritol-synthesis
#6
Tomasz Janek, Adam Dobrowolski, Anna Biegalska, Aleksandra M Mirończuk
BACKGROUND: Erythritol is a natural sweetener that is used in the food industry. It is produced as an osmoprotectant by bacteria and yeast. Due to its chemical properties, it does not change the insulin level in the blood, and therefore it can be safely used by diabetics. Previously, it has been shown that erythrose reductase (ER), which catalyzes the final step, plays a crucial role in erythritol synthesis. ER reduces erythrose to erythritol with NAD(P)H as a cofactor. Despite many studies on erythritol synthesis by Yarrowia lipolytica, the enzymes involved in this metabolic pathway have ever been described...
July 11, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28690604/phenol-is-the-initial-product-formed-during-growth-and-degradation-of-bromobenzene-by-tropical-marine-yeast-yarrowia-lipolytica-ncim-3589-via-an-early-dehalogenation-step
#7
Aakanksha A Vatsal, Smita S Zinjarde, Ameeta RaviKumar
Bromobenzene (BrB), a hydrophobic, recalcitrant organic compound, is listed by the environmental protection agencies as an environmental and marine pollutant having hepatotoxic, mutagenic, teratogenic, and carcinogenic effects. The tropical marine yeast Yarrowia lipolytica 3589 was seen to grow aerobically on BrB and displayed a maximum growth rate (μmax) of 0.04 h(-1). Furthermore, we also observed an increase in cell size and sedimentation velocity for the cells grown on BrB as compared to the glucose grown cells...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28688326/metabolic-peculiarities-of-the-citric-acid-overproduction-from-glucose-in-yeasts-yarrowia-lipolytica
#8
Svetlana V Kamzolova, Igor G Morgunov
Comparative study of 43 natural yeast strains belonging to 20 species for their capability for overproduction of citric acid (CA) from glucose under nitrogen limitation of cell growth was carried out. As a result, natural strain Yarrowia lipolytica VKM Y-2373 was selected. The effect of growth limitation by biogenic macroelements (nitrogen, phosphorus, or sulfur) on the CA production by the selected strain was studied. It was shown that yeasts Y. lipolytica grown under deficiency of nitrogen, phosphorus, or sulfur were able to excrete CA in industrially sufficient amounts (80-85g/L with the product yield (YCA) of 0...
June 28, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28686929/polyol-production-from-waste-materials-by-genetically-modified-yarrowia-lipolytica
#9
Magdalena Rakicka, Anna Biegalska, Waldemar Rymowicz, Adam Dobrowolski, Aleksandra M Mirończuk
Sugar alcohols (polyols) are sweeteners with many industrial applications. In this study, a fermentation process of polyol production based on waste substrates - raw industrial molasses and crude glycerol - was tested. The yeast strain Yarrowia lipolytica Wratislavia K1 was genetically modified by overexpression of the Saccharomyces cerevisiae SUC2 gene and overexpression of the native GUT1 gene. This process allowed for sucrose utilization and rapid glycerol assimilation by the engineered strain. In this study, the obtained strain AIB pAD-UTGut1 produced 100...
June 27, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28684180/enhancing-linalool-production-by-engineering-oleaginous-yeast-yarrowia-lipolytica
#10
Xuan Cao, Liu-Jing Wei, Jia-Yu Lin, Qiang Hua
In this study, stepwise increases in linalool production were obtained by combining metabolic engineering and process optimization of an unconventional oleaginous yeast Yarrowia lipolytica. The linalool synthetic pathway was successfully constructed by heterologously expressing a codon-optimized linalool synthase gene from Actinidia arguta in Y. lipolytica. To enhance linalool productivity, key genes involved in the mevalonate pathway were overexpressed in different combinations. Moreover, the overexpression of mutant ERG20(F88W-N119W) gene resulted in further linalool production...
June 23, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28662427/oral-mycoses-in-avian-scavengers-exposed-to-antibiotics-from-livestock-farming
#11
Aida Pitarch, Concha Gil, Guillermo Blanco
The exposure to antimicrobial pharmaceuticals as environmental contaminants can exert direct and indirect detrimental effects on health of wildlife. Fungal infections pose a major threat to domestic, captive-housed wild and free-ranging wild animals worldwide. However, little is known about their role in disease in birds in the wild. Here, we evaluated the incidence of thrush-like lesions in the oral cavity of wild nestling cinereous vultures (Aegypius monachus), griffon vultures (Gyps fulvus), Egyptian vultures (Neophron percnopterus) and golden eagles (Aquila chrysaetos) exposed to veterinary antibiotics via the consumption of medicated livestock carcasses...
June 26, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28651139/valorization-of-raw-glycerol-and-crustacean-waste-into-value-added-products-by-yarrowia-lipolytica
#12
Sara Magdouli, Tayssir Guedri, Rouissi Tarek, Satinder Kaur Brar, Jean François Blais
Crude glycerol has been widely investigated as a renewable carbon source for biodiesel production. In the present study, this feedstock was supplemented by various inducers: surfactants and oils to enhance lipid and lipase production by the newly isolated yeast, Yarrowia lipolytica. Results have shown that a culture medium composed of olive oil could enhance lipase activity at 25U/mL and lipid content up to 35% (w/w). The fortification of the medium with crustacean waste increased the lipase activity up to 38U/mL...
June 16, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28634240/leucine-biosynthesis-is-involved-in-regulating-high-lipid-accumulation-in-yarrowia-lipolytica
#13
Eduard J Kerkhoven, Young-Mo Kim, Siwei Wei, Carrie D Nicora, Thomas L Fillmore, Samuel O Purvine, Bobbie-Jo Webb-Robertson, Richard D Smith, Scott E Baker, Thomas O Metz, Jens Nielsen
The yeast Yarrowia lipolytica is a potent accumulator of lipids, and lipogenesis in this organism can be influenced by a variety of factors, such as genetics and environmental conditions. Using a multifactorial study, we elucidated the effects of both genetic and environmental factors on regulation of lipogenesis in Y. lipolytica and identified how two opposite regulatory states both result in lipid accumulation. This study involved comparison of a strain overexpressing diacylglycerol acyltransferase (DGA1) with a control strain grown under either nitrogen or carbon limitation conditions...
June 20, 2017: MBio
https://www.readbyqxmd.com/read/28631058/expression-and-characterization-of-glucose-oxidase-from-aspergillus-niger-in-yarrowia-lipolytica
#14
Fatemeh Khadivi Derakshan, Farshad Darvishi, Mehrouz Dezfulian, Catherine Madzak
Glucose oxidase (GOX) is currently used in clinical, pharmaceutical, food and chemical industries. The aim of this study was expression and characterization of Aspergillus niger glucose oxidase gene in the yeast Yarrowia lipolytica. For the first time, the GOX gene of A. niger was successfully expressed in Y. lipolytica using a mono-integrative vector containing strong hybrid promoter and secretion signal. The highest total glucose oxidase activity was 370 U/L after 7 days of cultivation. An innovative method was used to cell wall disruption in current study, and it could be recommended to use for efficiently cell wall disruption of Y...
August 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/28630295/application-of-metabolic-controls-for-the-maximization-of-lipid-production-in-semicontinuous-fermentation
#15
Jingyang Xu, Nian Liu, Kangjian Qiao, Sebastian Vogg, Gregory Stephanopoulos
Acetic acid can be generated through syngas fermentation, lignocellulosic biomass degradation, and organic waste anaerobic digestion. Microbial conversion of acetate into triacylglycerols for biofuel production has many advantages, including low-cost or even negative-cost feedstock and environmental benefits. The main issue stems from the dilute nature of acetate produced in such systems, which is costly to be processed on an industrial scale. To tackle this problem, we established an efficient bioprocess for converting dilute acetate into lipids, using the oleaginous yeast Yarrowia lipolytica in a semicontinuous system...
July 3, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28627452/engineering-of-unconventional-yeast-yarrowia-lipolytica-for-efficient-succinic-acid-production-from-glycerol-at-low-ph
#16
Zhiyong Cui, Cuijuan Gao, Jiaojiao Li, Jin Hou, Carol Sze Ki Lin, Qingsheng Qi
Yarrowia lipolytica is considered as a potential candidate for succinic acid production because of its innate ability to accumulate citric acid cycle intermediates and its tolerance to acidic pH. Previously, a succinate-production strain was obtained through the deletion of succinate dehydrogenase subunit encoding gene Ylsdh5. However, the accumulation of by-product acetate limited further improvement of succinate production. Meanwhile, additional pH adjustment procedure increased the downstream cost in industrial application...
June 13, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28608278/identification-and-characterization-of-eyk1-a-key-gene-for-erythritol-catabolism-in-yarrowia-lipolytica
#17
F Carly, H Gamboa-Melendez, M Vandermies, C Damblon, J M Nicaud, P Fickers
Erythritol is a four-carbon sugar alcohol synthesized by osmophilic yeasts, such as Yarrowia lipolytica, in response to osmotic stress. This metabolite has application as food additive due to its sweetening properties. Although Y. lipolytica can produce erythritol at a high level from glycerol, it is also able to consume it as carbon source. This ability negatively affects erythritol productivity and represents a serious drawback for the development of an efficient erythritol production process. In this study, we have isolated by insertion mutagenesis a Y...
June 12, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28591644/co-expression-of-glucose-6-phosphate-dehydrogenase-and-acyl-coa-binding-protein-enhances-lipid-accumulation-in-the-yeast-yarrowia-lipolytica
#18
Evgeniya Y Yuzbasheva, Elizaveta B Mostova, Natalia I Andreeva, Tigran V Yuzbashev, Ivan A Laptev, Tatiana I Sobolevskaya, Sergey P Sineoky
The oleaginous yeast Yarrowia lipolytica is a convenient model for investigating lipid biosynthesis and for engineering high lipid accumulated strains. In this organism, the pentose phosphate pathway is the major source of NADPH for lipid biosynthesis. Thus, we over-expressed gene encoding NADP(+)-dependent glucose-6-phosphate dehydrogenase (ZWF1) in a strain deficient in peroxisome biogenesis. However, this strategy suppressed growth during cultivation under lipogenic conditions and did not significantly increase lipid accumulation...
June 4, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28589225/characterization-of-the-newly-isolated-%C3%AF-oxidizing-yeast-candida-sorbophila-ds02-and-its-potential-applications-in-long-chain-dicarboxylic-acid-production
#19
Heeseok Lee, Yohanes Eko Chandra Sugiharto, Seunghoon Lee, Gyuyeon Park, Changpyo Han, Hyeran Jang, Wooyoung Jeon, Heejoon Park, Jungoh Ahn, Kyungbo Kang, Hongwoen Lee
α, ω-Dicarboxylic acids (DCAs) are multipurpose chemicals widely used in polymers, perfumes, plasticizers, lubricants, and adhesives. The biotransformation of DCAs from alkanes and fatty acids by microorganisms has attracted recent interest, since synthesis via chemical oxidation causes problems in terms of the environment and safety. We isolated an ω-oxidizing yeast from a wastewater disposal facility of a petrochemical factory by chemostat enrichment culture. The haploid strain identified as Candida sorbophila DS02 grew on glucose and dodecane, exhibiting greater cell shrinkage on the latter...
June 6, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28585817/production-of-medium-chain-fatty-acids-by-yarrowia-lipolytica-combining-molecular-design-and-talen-to-engineer-the-fatty-acid-synthase
#20
Coraline Rigouin, Marc Gueroult, Christian Croux, Gwendoline Dubois, Vinciane Borsenberger, Sophie Barbe, Alain Marty, Fayza Daboussi, Isabelle André, Florence Bordes
Yarrowia lipolytica is a promising organism for the production of lipids of biotechnological interest and particularly for biofuel. In this study, we engineered the key enzyme involved in lipid biosynthesis, the giant multifunctional fatty acid synthase (FAS), to shorten chain length of the synthesized fatty acids. Taking as starting point that the ketoacyl synthase (KS) domain of Yarrowia lipolytica FAS is directly involved in chain length specificity, we used molecular modeling to investigate molecular recognition of palmitic acid (C16 fatty acid) by the KS...
June 21, 2017: ACS Synthetic Biology
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