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https://www.readbyqxmd.com/read/28443314/cutaneous-yarrowia-lipolytica-infection-in-an-immunocompetent-woman
#1
Alan S Boyd, Lee Wheless, Bobbi G Brady, Darrel Ellis
No abstract text is available yet for this article.
May 2017: JAAD Case Reports
https://www.readbyqxmd.com/read/28419226/sugar-versus-fat-elimination-of-glycogen-storage-improves-lipid-accumulation-in-yarrowia-lipolytica
#2
Govindprasad Bhutada, Martin Kavšcek, Rodrigo Ledesma-Amaro, Stéphane Thomas, Gerald N Rechberger, Jean-Marc Nicaud, Klaus Natter
Triacylglycerol (TAG) and glycogen are the two major metabolites for carbon storage in most eukaryotic organisms. We investigated the glycogen metabolism of the oleaginous Y. lipolytica and found that this yeast accumulates up to 16% glycogen in its biomass. Assuming that elimination of glycogen synthesis would result in an improvement of lipid accumulation, we characterized and deleted the single gene coding for glycogen synthase, YlGSY1. The mutant was grown under lipogenic conditions with glucose and glycerol as substrates and we obtained up to 60% improvement in TAG accumulation compared to the wild type strain...
April 17, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28414174/iterative-integration-of-multiple-copy-pathway-genes-in-yarrowia-lipolytica-for-heterologous-%C3%AE-carotene-production
#3
Shuliang Gao, Yangyang Tong, Li Zhu, Mei Ge, Yian Zhang, Daijie Chen, Yu Jiang, Sheng Yang
β-Carotene is a terpenoid molecule with high hydrophobicity that is often used as an additive in foods and feed. Previous work has demonstrated the heterologous biosynthesis of β-carotene from an intrinsic high flux of acetyl-CoA in 12 steps through 11 genes in Yarrowia lipolytica. Here, an efficient biosynthetic pathway capable of producing 100-fold more β-carotene than the baseline construct was generated using strong promoters and multiple gene copies for each of the 12 steps. Using fed-batch fermentation with an optimized medium, the engineered pathway could produce 4g/L β-carotene, which was stored in lipid droplets within engineered Y...
April 13, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28398122/investigating-proteome-and-transcriptome-defense-response-of-apples-induced-by-yarrowia-lipolytica
#4
Hongyin Zhang, Liangliang Chen, Yiwen Sun, Lina Zhao, Xiangfeng Zheng, Qiya Yang, Xiaoyun Zhang
A better understanding of the mode of action of postharvest biocontrol agents on fruit surfaces is critical for the advancement of successful implementation of postharvest biocontrol products. This is due to the increasing importance of biological control of postharvest diseases over chemical and other control methods. However, most of the mechanisms involved in biological control remain unknown and need to be explored. Yarrowia lipolytica significantly inhibited blue mold decay of apples caused by Penicillium expansum...
April 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28388696/the-use-of-serine-protease-from-yarrowia-lipolytica-yeast-in-the-production-of-biopeptides-from-denatured-egg-white-proteins
#5
Marta Pokora, Aleksandra Zambrowicz, Agnieszka Zabłocka, Anna Dąbrowska, Marek Szołtysik, Konrad Babij, Ewelina Eckert, Tadeusz Trziszka, Józefa Chrzanowska
Deriving non-conventional enzymes from cheaper sources than those used for commercially available enzymes may result in the production of hydrolysates with beneficial features, while drastically reducing the cost of hydrolysis. This is especially significant for enzymatic hydrolysis as a method of protein waste utilization. We have previously described the ability of non-commercial serine protease from Yarrowia lipolytica yeast to produce/release bioactive peptides from egg white protein by-products (EP). The enzymatic hydrolysis of EP was carried out for 24 h using the serine protease at an enzyme: substrate ratio of 1:30 (w/w)...
April 7, 2017: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/28384620/recovering-traditional-raw-milk-tetilla-cheese-flavour-and-sensory-attributes-by-using-kocuria-varians-and-yarrowia-lipolytica-adjunct-cultures
#6
J A Centeno, J I Garabal, F Docampo, J M Lorenzo, J Carballo
The rationale of the present study was to evaluate the potential of microbial adjunct cultures including Kocuria varians and/or Yarrowia lipolytica strains in the recovery of the typical sensory profile of traditional (raw-milk) Tetilla cheese. Four batches of Tetilla cheese, a short ripened cows' milk cheese produced in Galicia (NW Spain), were made in duplicate from pasteurized milk inoculated with different microbial cultures. A control batch was manufactured by adding a mesophilic commercial D-starter only...
March 22, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28376264/synthesis-and-structure-activity-relationships-of-novel-benzylamine-type-antifungals-as-butenafine-related-antimycotics
#7
Jürgen Krauss, Martina Stadler, Franz Bracher
Benzylamine-type antimycotics like naftifine, butenafine, or terbinafine are a well-known class of antimycotics since the 1980s. The following paper describes the synthesis and biological evaluation of a series of novel benzylamine-type antimycotics characterized by an isooctyl side chain and various substituents at the benzylamine moiety. The compounds were prepared from benzaldehyde derivatives and 2-amino-6-methylheptane by reductive amination with sodium triacetoxyborohydride and subsequent precipitation with hydrogen chloride...
April 4, 2017: Archiv der Pharmazie
https://www.readbyqxmd.com/read/28357621/improved-campesterol-production-in-engineered-yarrowia-lipolytica-strains
#8
Yu Zhang, Ying Wang, Mingdong Yao, Hong Liu, Xiao Zhou, Wenhai Xiao, Yingjin Yuan
OBJECTIVES: To engineer Yarrowia lipolytica for improving the heterologous production of campesterol (a key precursor to manufacture pharmaceutical steroids). RESULTS: By screening 7-dehydrocholesterol reductase (DHCR7) from diverse species, DHCR7 from Danio rerio was the best candidate for campesterol synthesis. Overexpression of ACL (ATP: citrate lyase) or POX2 (peroxisome acyl-CoA oxidase 2) were key to improving campesterol production. The highest yield of campesterol was 942 mg/l was with the strain overexpressing POX2 in a 5 l bioreactor via high cell density fermentation process with a restricted supply of carbon sourc, sunflower seed oil...
March 29, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28357546/a-metabolic-engineering-strategy-for-producing-conjugated-linoleic-acids-using-the-oleaginous-yeast-yarrowia-lipolytica
#9
Nabila Imatoukene, Jonathan Verbeke, Athanasios Beopoulos, Abdelghani Idrissi Taghki, Brigitte Thomasset, Claude-Olivier Sarde, Maurice Nonus, Jean-Marc Nicaud
Conjugated linoleic acids (CLAs) have been found to have beneficial effects on human health when used as dietary supplements. However, their availability is limited because pure, chemistry-based production is expensive, and biology-based fermentation methods can only create small quantities. In an effort to enhance microbial production of CLAs, four genetically modified strains of the oleaginous yeast Yarrowia lipolytica were generated. These mutants presented various genetic modifications, including the elimination of β-oxidation (pox1-6∆), the inability to store lipids as triglycerides (dga1∆ dga2∆ are1∆ lro1∆), and the overexpression of the Y...
March 29, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28352301/functional-overexpression-of-genes-involved-in-erythritol-synthesis-in-the-yeast-yarrowia-lipolytica
#10
Aleksandra M Mirończuk, Anna Biegalska, Adam Dobrowolski
BACKGROUND: Erythritol, a four-carbon polyol synthesized by microorganisms as an osmoprotectant, is a natural sweetener produced on an industrial scale for decades. Despite the fact that the yeast Yarrowia lipolytica has been reported since the 1970s as an erythritol producer, the metabolic pathway of this polyol has never been characterized. It was shown that erythritol synthesis in yeast occurs via the pentose phosphate pathway (PPP). The oleaginous yeast Y. lipolytica is a good host for converting inexpensive glycerol into a value-added product such as erythritol...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28346372/-bligh-and-dyer-and-folch-methods-for-solid-liquid-liquid-extraction-of-lipids-from-microorganisms-comprehension-of-solvatation-mechanisms-and-towards-substitution-with-alternative-solvents
#11
Cassandra Breil, Maryline Abert Vian, Thomas Zemb, Werner Kunz, Farid Chemat
Bligh and Dyer (B & D) or Folch procedures for the extraction and separation of lipids from microorganisms and biological tissues using chloroform/methanol/water have been used tens of thousands of times and are "gold standards" for the analysis of extracted lipids. Based on the Conductor-like Screening MOdel for realistic Solvatation (COSMO-RS), we select ethanol and ethyl acetate as being potentially suitable for the substitution of methanol and chloroform. We confirm this by performing solid-liquid extraction of yeast (Yarrowia lipolytica IFP29) and subsequent liquid-liquid partition-the two steps of routine extraction...
March 27, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28341907/utilization-of-inulin-containing-waste-in-industrial-fermentations-to-produce-biofuels-and-bio-based-chemicals
#12
REVIEW
Stephen R Hughes, Nasib Qureshi, Juan Carlos López-Núñez, Marjorie A Jones, Joshua M Jarodsky, Luz Ángela Galindo-Leva, Mitchell R Lindquist
Inulins are polysaccharides that belong to an important class of carbohydrates known as fructans and are used by many plants as a means of storing energy. Inulins contain 20 to several thousand fructose units joined by β-2,1 glycosidic bonds, typically with a terminal glucose unit. Plants with high concentrations of inulin include: agave, asparagus, coffee, chicory, dahlia, dandelion, garlic, globe artichoke, Jerusalem artichoke, jicama, onion, wild yam, and yacón. To utilize inulin as its carbon and energy source directly, a microorganism requires an extracellular inulinase to hydrolyze the glycosidic bonds to release fermentable monosaccharides...
April 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28303649/transforming-sugars-into-fat-lipid-biosynthesis-using-different-sugars-in-yarrowia-lipolytica
#13
Piotr Hapeta, Magdalena Rakicka, Remi Dulermo, Heber Gamboa-Meléndez, Anne-Marie Cruz-Le Coq, Jean-Marc Nicaud, Zbigniew Lazar
In an era of ever-increasing energy demands, a promising technology is being developed: the use of oleaginous microorganisms such as Yarrowia lipolytica to convert waste materials into biofuels. Here, we constructed two Y. lipolytica strains that displayed both increased lipid accumulation and more efficient use of biomass-derived sugars, including glucose, fructose, galactose and inulin. The first strain, Y. lipolytica YLZ150, was derived from the French wild-type strain W29. It had inhibited triacylglycerol mobilization (∆tgl4) and β-oxidation (∆pox1-6), and it overexpressed GPD1, DGA2, HXK1, the native Leloir pathway, SUC2 from Saccharomyces cerevisiae and INU1 from Kluyveromyces marxianus...
March 17, 2017: Yeast
https://www.readbyqxmd.com/read/28295166/engineering-oxidative-stress-defense-pathways-to-build-a-robust-lipid-production-platform-in-yarrowia-lipolytica
#14
Peng Xu, Kangjian Qiao, Gregory Stephanopoulos
Microbially derived lipids have recently attracted renewed interests due to their broad applications in production of green diesels, cosmetic additives, and oleochemicals. Metabolic engineering efforts have targeted a large portfolio of biosynthetic pathways to efficiently convert sugar to lipids in oleaginous yeast. In the engineered overproducing strains, endogenous cell metabolism typically generates harmful electrophilic molecules that compromise cell fitness and productivity. Lipids, particularly unsaturated fatty acids, are highly susceptible to oxygen radical attack and the resulting oxidative species are detrimental to cell metabolism and limit lipid productivity...
March 14, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28289912/production-of-%C3%AE-carotene-by-expressing-a-heterologous-multifunctional-carotene-synthase-in-yarrowia-lipolytica
#15
Shuliang Gao, Yangyang Tong, Li Zhu, Mei Ge, Yu Jiang, Daijie Chen, Sheng Yang
OBJECTIVES: To obtain functional expression of a heterologous multifunctional carotene synthase containing phytoene synthase, phytoene dehydrogenase, and lycopene β-cyclase activities encoded by carS from Schizochytrium sp. in order to allow Yarrowia lipolytica to produce β-carotene. RESULTS: To increase the integration efficiency of a 3.8 kb carS under the control of P GPD promoter with a 2 kb selection marker, ura3, along with a geranylgeranyl diphosphate synthase (GGS1) expression cassette (~10 kb in total), was inserted into the Y...
March 13, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28282268/construction-of-recombinant-yarrowia-lipolytica-and-its-application-in-bio-transformation-of-lignocellulose
#16
Hui-Ting Song, Yi-Min Yang, Ding-Kang Liu, Xiao-Qing Xu, Wen-Jing Xiao, Zi-Lu Liu, Wu-Cheng Xia, Chao-Ying Wang, Xiao Yu, Zheng-Bing Jiang
Lignocellulose is a polysaccharide and an abundant biomass resource that widely exists in grains, beans, rice, and their by-products. Over 10 million tons of lignocellulose resources and processing products are produced every year in China. Three recombinant Y. lipolytica strains with cellulase (β-glucosidase, endoglucanase and cellobiohydrolase) were constructed. The enzymatic activities of these enzymes were 14.181 U/mL, 16.307 U/mL, and 17.391 U/mL, respectively. The whole cell cellulases were used for a stover bio-transformation...
February 17, 2017: Bioengineered
https://www.readbyqxmd.com/read/28244372/action-mechanisms-involved-in-the-bioprotective-effect-of-lactobacillus-harbinensis-k-v9-3-1-np-against-yarrowia-lipolytica-in-fermented-milk
#17
Sophie Mieszkin, Nolwenn Hymery, Stella Debaets, Emmanuel Coton, Gwenaelle Le Blay, Florence Valence, Jérôme Mounier
The use of lactic acid bacteria (LAB) as bioprotective cultures can be an alternative to chemical preservatives or antibiotic to prevent fungal spoilage in dairy products. Among antifungal LAB, Lactobacillus harbinensis K.V9.3.1Np showed a remarkable antifungal activity for the bioprotection of fermented milk without modifying their organoleptic properties (Delavenne et al., 2015). The aim of the present study was to elucidate the action mechanism of this bioprotective strain against the spoilage yeast Yarrowia lipolytica...
May 2, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28239417/erratum-to-a-molecular-genetic-toolbox-for-yarrowia-lipolytica
#18
Erin L Bredeweg, Kyle R Pomraning, Ziyu Dai, Jens Nielsen, Eduard J Kerkhoven, Scott E Baker
[This corrects the article DOI: 10.1186/s13068-016-0687-7.].
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28217858/golden-gate-assembly-system-dedicated-to-complex-pathway-manipulation-in-yarrowia-lipolytica
#19
Ewelina Celińska, Rodrigo Ledesma-Amaro, Macarena Larroude, Tristan Rossignol, Cyrille Pauthenier, Jean-Marc Nicaud
In this study, we have adopted Golden Gate modular cloning strategy to develop a robust and versatile DNA assembly platform for the nonconventional yeast Yarrowia lipolytica. To this end, a broad set of destination vectors and interchangeable building blocks have been constructed. The DNA modules were assembled on a scaffold of predesigned 4 nt overhangs covering three transcription units (each bearing promoter, gene and terminator), selection marker gene and genomic integration targeting sequences, constituting altogether thirteen elements...
March 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28217743/regulation-of-nitrogen-metabolism-by-gata-zinc-finger-transcription-factors-in-yarrowia-lipolytica
#20
Kyle R Pomraning, Erin L Bredeweg, Scott E Baker
Fungi accumulate lipids in a manner dependent on the quantity and quality of the nitrogen source on which they are growing. In the oleaginous yeast Yarrowia lipolytica, growth on a complex source of nitrogen enables rapid growth and limited accumulation of neutral lipids, while growth on a simple nitrogen source promotes lipid accumulation in large lipid droplets. Here we examined the roles of nitrogen catabolite repression and its regulation by GATA zinc finger transcription factors on lipid metabolism in Y...
January 2017: MSphere
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