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https://www.readbyqxmd.com/read/29238402/expressing-accessory-proteins-in-cellulolytic-yarrowia-lipolytica-to-improve-the-conversion-yield-of-recalcitrant-cellulose
#1
Zhong-Peng Guo, Sophie Duquesne, Sophie Bozonnet, Jean-Marc Nicaud, Alain Marty, Michael Joseph O'Donohue
Background: A recently constructed cellulolytic Yarrowia lipolytica is able to grow efficiently on an industrial organosolv cellulose pulp, but shows limited ability to degrade crystalline cellulose. In this work, we have further engineered this strain, adding accessory proteins xylanase II (XYNII), lytic polysaccharide monooxygenase (LPMO), and swollenin (SWO) from Trichoderma reesei in order to enhance the degradation of recalcitrant substrate. Results: The production of EG I was enhanced using a promoter engineering strategy...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29224624/baeyer-villiger-monooxygenases-from-yarrowia-lipolytica-catalyze-preferentially-sulfoxidations
#2
Sven Bordewick, Andy Beier, Kathleen Balke, Uwe T Bornscheuer
Nine new putative Baeyer-Villiger monooxygenase encoding genes were identified in the eukaryote Yarrowia lipolytica and eight were subsequently cloned and expressed. These enzymes, Yarrowia monooxygenases A-H (YMOA-H), were used in biocatalysis reactions with ketones, sulfides and sulfoxides as substrates. YMOB converts ketones and sulfides, albeit with low activities. However, YMOA did not convert any of the tested ketone substrates, but showed activity towards sulfides and sulfoxides and also showed very high stereoselectivity...
February 2018: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/29198706/structural-insight-into-the-substrate-specificity-of-acyl-coa-oxidase1-from-yarrowia-lipolytica-for-short-chain-dicarboxylyl-coas
#3
Sangwoo Kim, Kyung-Jin Kim
Acyl-CoA oxidase (ACOX) plays an important role in fatty acid degradation. The enzyme catalyzes the first reaction in peroxisomal fatty acid β-oxidation by reducing acyl-CoA to 2-trans-enoyl-CoA. The yeast Yarrowia lipolytica is able to utilize fatty acids, fats, and oil as carbon sources to produce valuable bioproducts. We determined the crystal structure of ACOX1 from Y. lipolytica (YlACOX1) at a resolution of 2.5 Å. YlACOX1 forms a homodimer, and the monomeric structure is composed of four domains, the Nα, Nβ, Cα1, and Cα2...
November 30, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29195120/advances-in-cellulosic-conversion-to-fuels-engineering-yeasts-for-cellulosic-bioethanol-and-biodiesel-production
#4
REVIEW
Ja Kyong Ko, Sun-Mi Lee
Cellulosic fuels are expected to have great potential industrial applications in the near future, but they still face technical challenges to become cost-competitive fuels, thus presenting many opportunities for improvement. The economical production of viable biofuels requires metabolic engineering of microbial platforms to convert cellulosic biomass into biofuels with high titers and yields. Fortunately, integrating traditional and novel engineering strategies with advanced engineering toolboxes has allowed the development of more robust microbial platforms, thus expanding substrate ranges...
November 27, 2017: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/29189924/immobilization-in-polyvinyl-alcohol-hydrogel-enhances-yeast-storage-stability-and-reusability-of-recombinant-laccase-producing-s-cerevisiae
#5
Klára Herkommerová, Jana Zemančíková, Hana Sychrová, Zuzana Antošová
OBJECTIVES: To improve the storage stability and reusability of various yeast strains and species by immobilization in polyvinyl alcohol (PVA) hydrogel particles. RESULTS: Debaryomyces hansenii, Pichia sorbitophila, Saccharomyces cerevisiae, Yarrowia lipolytica, and Zygosaccharomyces rouxii were immobilized in PVA particles using LentiKats technology and stored in sterile water at 4 °C. The immobilization improved the survival of all species; however, the highest storage stability was achieved for S...
November 30, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/29188186/yalibricks-a-versatile-genetic-toolkit-for-streamlined-and-rapid-pathway-engineering-in-yarrowia-lipolytica
#6
Lynn Wong, Jake Engel, Erqing Jin, Benjamin Holdridge, Peng Xu
Effective metabolic engineering of microorganisms relies on balanced expression of both heterologous and endogenous genes to channel metabolic flux towards products of interest while achieving reasonable biomass buildup. To facilitate combinatorial pathway engineering and facile genetic operation, we engineered a set of modular cloning vectors compatible with BioBrick standards, called YaliBricks, to allow for rapid assembly of multigene pathways with customized genetic control elements (promoters, intronic sequences and terminators) in the oleaginous yeast Yarrowia lipolytica...
December 2017: Metabolic Engineering Communications
https://www.readbyqxmd.com/read/29184240/essential-oils-as-antibacterial-agents-against-food-borne-pathogens-are-they-really-as-useful-as-they-are-claimed-to-be
#7
M I S Santos, S R Martins, C S C Veríssimo, M J C Nunes, A I G Lima, R M S B Ferreira, L Pedroso, I Sousa, M A S S Ferreira
Most studies evaluating the use of essential oils (EO) as antibacterial agents focus mainly on minimal inhibitory concentrations (MIC) rather than minimal bactericidal concentrations (MBC). In this work, we compared MICs and MBCs of EO from condiment plants commonly used in Mediterranean Europe, namely Origanum vulgare, Salvia lavandulaefolia, Salvia officinalis, Salvia sclarea and Rosmarinus officinalis, aiming to evaluate their application as disinfecting agents in minimally processed produce. Outbreaks-related pathogens such as Listeria monocytogenes, Pseudomonas aeruginosa and Yarrowia lipolytica were used...
December 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29167664/metabolic-engineering-of-oleaginous-yeasts-for-production-of-fuels-and-chemicals
#8
REVIEW
Shuobo Shi, Huimin Zhao
Oleaginous yeasts have been increasingly explored for production of chemicals and fuels via metabolic engineering. Particularly, there is a growing interest in using oleaginous yeasts for the synthesis of lipid-related products due to their high lipogenesis capability, robustness, and ability to utilize a variety of substrates. Most of the metabolic engineering studies in oleaginous yeasts focused on Yarrowia that already has plenty of genetic engineering tools. However, recent advances in systems biology and synthetic biology have provided new strategies and tools to engineer those oleaginous yeasts that have naturally high lipid accumulation but lack genetic tools, such as Rhodosporidium, Trichosporon, and Lipomyces...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29164392/maturation-and-cytokine-pattern-of-human-dendritic-cells-in-response-to-different-yeasts
#9
Silvia Boschi Bazan, Barbara Walch-Rückheim, Manfred J Schmitt, Frank Breinig
Activated dendritic cells (DC) induce and polarize T-cell responses by expression of distinct maturation markers and cytokines. This study systematically investigated the capacity of different biotechnically relevant yeast species and strains including Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces lactis, Pichia pastoris, Hansenula polymorpha, Yarrowia lipolytica, and Candida glabrata to initiate maturation of human DC. As important prerequisite for T-cell activation, all yeasts were shown to effectively induce, though to a different extent, the expression of the activation marker CD83, the co-stimulatory molecules CD80, CD86, CD54, CD58, and CD40, as well as the antigen-presenting molecules MHCs I and II...
November 21, 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/29150774/co-expression-of-exo-inulinase-and-endo-inulinase-genes-in-the-oleaginous-yeast-yarrowia-lipolytica-for-efficient-single-cell-oil-production-from-inulin
#10
Nianci Shi, Weian Mao, Xiaoxia He, Zhe Chi, Zhenming Chi, Guanglei Liu
Yarrowia lipolytica is a promising platform for the single cell oil (SCO) production. In this study, a transformant X+N8 in which exo- and endo-inulinase genes were co-expressed could produce an inulinase activity of 124.33 U/mL within 72 h. However, the inulinase activity of a transformant X2 carrying a single exo-inulinase gene was only 47.33 U/mL within 72 h. Moreover, the transformant X+N8 could accumulate 48.13% (w/w) SCO from inulin and the cell dry weight reached 13.63 g/L within 78 h, which were significantly higher than those of the transformant X2 (41...
November 18, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29150499/understanding-functional-roles-of-native-pentose-specific-transporters-for-activating-dormant-pentose-metabolism-in-yarrowia-lipolytica
#11
Seunghyun Ryu, Cong T Trinh
Pentoses including xylose and arabinose are the second-most prevalent sugars of lignocellulosic biomass that can be harnessed for biological conversion. Although Yarrowia lipolytica has emerged as a promising industrial microorganism for production of high-value chemicals and biofuels, its native pentose metabolism is poorly understood. Our previous study demonstrated that Y. lipolytica (ATCC MYA-2613) has endogenous enzymes for D-xylose assimilation, but inefficient xylitol dehydrogenase causes Y. lipolytica to assimilate xylose poorly...
November 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29141191/microbial-lipids-and-added-value-metabolites-production-by-yarrowia-lipolytica-from-pork-lard
#12
Marlene Lopes, Andreia S Gomes, Carla M Silva, Isabel Belo
Yarrowia lipolytica is a suitable microbial platform to convert low-value hydrophobic substrates into microbial oils and other important metabolites. In this work, this yeast species was used to simultaneously synthetize ex novo lipids and produce citric acid and lipase from animal fat (pork lard) adding higher value to the low-cost fatty substrate. The effect of pH, lard concentration, arabic gum concentration and oxygen mass transfer rate (OTR) on lipids accumulation on Y. lipolytica batch cultures was assessed by an experimental design based on Taguchi method...
November 12, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/29129976/using-odd-alkanes-as-a-carbon-source-to-increase-the-content-of-nutritionally-important-fatty-acids-in-candida-krusei-trichosporon-cutaneum-and-yarrowia-lipolytica
#13
Olga Matatkova, Lucia Gharwalova, Michal Zimola, Tomas Rezanka, Jan Masak, Irena Kolouchova
We investigated the possibility of utilizing unusual carbon sources by three yeast strains: Candida krusei DBM 2136, Trichosporon cutaneum CCY 30-5-10, and Yarrowia lipolytica CCY 30-26-36. These strains are characterized by high biomass yield, ability to accumulate high amounts of lipids, and their potential as producers of dietetically important fatty acids. The aim of this work was the production of nutritionally important fatty acids by utilization of n-alkanes with an odd number of carbon atoms, alone and in combination with glucose and subsequent analysis of microbial lipids accumulation and fatty acid profile...
2017: International Journal of Analytical Chemistry
https://www.readbyqxmd.com/read/29127883/metabolic-engineering-of-yeast-for-lignocellulosic-biofuel-production
#14
REVIEW
Yong-Su Jin, Jamie Hd Cate
Production of biofuels from lignocellulosic biomass remains an unsolved challenge in industrial biotechnology. Efforts to use yeast for conversion face the question of which host organism to use, counterbalancing the ease of genetic manipulation with the promise of robust industrial phenotypes. Saccharomyces cerevisiae remains the premier host for metabolic engineering of biofuel pathways, due to its many genetic, systems and synthetic biology tools. Numerous engineering strategies for expanding substrate ranges and diversifying products of S...
November 8, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/29127027/a-mitochondrial-manganese-superoxide-dismutase-involved-in-innate-immunity-is-essential-for-the-survival-of-chlamys-farreri
#15
Mengqiang Wang, Baojie Wang, Keyong Jiang, Mei Liu, Xiaowei Shi, Lei Wang
Superoxide dismutase (SOD) ubiquitously found in both prokaryotes and eukaryotes functions as the first and essential enzyme in the antioxidant system. In the present study, a manganese SOD (designated as CfmtMnSOD) was cloned from Zhikong scallop Chlamys farreri. The complete cDNA sequence of CfmtMnSOD contained a 681 bp open reading frame (ORF), encoding a peptide of 226 amino acids. A SOD_Fe_N domain and a SOD_Fe_C domain were found in the deduced amino acid sequence of CfmtMnSOD. The mRNA transcripts of CfmtMnSOD were constitutively expressed in all the tested tissues, including gill, gonad, hepatopancreas, hemocytes, mantle and muscle, with the highest expression level in hemocytes...
November 7, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29112152/yarrowia-lipolytica-extracellular-lipase-lip2-as-biocatalyst-for-the-ring-opening-polymerization-of-%C3%AE%C2%B5-caprolactone
#16
Karla A Barrera-Rivera, Antonio Martínez-Richa
Yarrowia lipolytica (YL) is a "non-conventional" yeast that is capable of producing important metabolites. One of the most important products that is secreted by this microorganism is lipase, a ubiquitous enzyme that has considerable industrial potential and can be used as a biocatalyst in the pharmaceutical, food, and environmental industries. In this work, Yarrowia lipolytica lipase (YLL) was immobilized on Lewatit and Amberlite beads and is used in the enzymatic ring-opening polymerization (ROP) of cyclic esters in the presence of different organic solvents...
November 7, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29111498/transcriptomic-response-of-debaryomyces-hansenii-during-mixed-culture-in-a-liquid-model-cheese-medium-with-yarrowia-lipolytica
#17
Reine Malek, Pascal Bonnarme, Françoise Irlinger, Pascale Frey-Klett, Djamila Onésime, Julie Aubert, Valentin Loux, Jean-Marie Beckerich
Yeasts play a crucial role in cheese ripening. They contribute to the curd deacidification, the establishment of acid-sensitive bacterial communities, and flavour compounds production via proteolysis and catabolism of amino acids (AA). Negative yeast-yeast interaction was observed between the yeast Yarrowia lipolytica 1E07 (YL1E07) and the yeast Debaryomyces hansenii 1L25 (DH1L25) in a model cheese but need elucidation. YL1E07 and DH1L25 were cultivated in mono and co-cultures in a liquid synthetic medium (SM) mimicking the cheese environment and the growth inhibition of DH1L25 in the presence of YL1E07 was reproduced...
October 24, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29106510/yarrowialipolytica-a-model-yeast-for-citric-acid-production
#18
Ema Cavallo, Hernán Charreau, Patricia Cerrutti, María Laura Foresti
Every year more than 2 million tons of citric acid (CA) are produced around the world for industrial uses. Although initially extracted from citrus, the low profitability of the process and the increasing demand soon stimulated the search for more efficient methods to produce CA. Currently, most world CA demand (99%) is satisfied by fermentations with microorganisms, especially filamentous fungi and yeasts. CA production with yeasts has certain advantages over molds (e.g. higher productivity and easier cultivation), which in the last two decades have triggered a clear increase in publications and patents devoted to the use of yeasts in this field...
November 2, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/29102548/modulation-of-the-glycerol-phosphate-availability-led-to-concomitant-reduction-in-the-citric-acid-excretion-and-increase-in-lipid-content-and-yield-in-yarrowia-lipolytica
#19
Rana Sagnak, Sandrine Cochot, Carole Molina-Jouve, Jean-Marc Nicaud, Stéphane E Guillouet
In order to improve TriAcylGycerol (TAG) lipids accumulation in the yeast Yarrowia lipolytica on glucose, double over-expression of the major acyl-CoA:diacylglycerol acyltransferase encoding gene (ylDGA2) and of the glycerol-phosphate dehydrogenase encoding gene (ylGPD1) was carried out. The genes were over-expressed in a strain impaired for the mobilization of the accumulated lipids, through the deletion of the genes encoding acyl-coenzyme A oxidases (POX1-6 genes) and the deletion of the very efficient lipase attached to the lipid bodies, encoded by ylTGL4...
November 2, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/29095200/emerging-multidrug-resistant-candida-species
#20
Arnaldo L Colombo, João N de Almeida Júnior, Jesus Guinea
PURPOSE OF REVIEW: To describe the epidemiology, strategies for early detection, and clinical management of infections caused by the most commonly found multidrug-resistant (MDR) Candida spp. RECENT FINDINGS: Increasing numbers of reports describing invasive infections by MDR Candida auris and Candida glabrata has been reported in medical centers worldwide. SUMMARY: We checked all papers published along the last 10 years describing epidemiological, diagnostic, and clinical aspects of infections by MDR Candida spp...
December 2017: Current Opinion in Infectious Diseases
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