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Mathiazhakan Kuttiraja, Ayed Dhouha, Rajeshwar Dayal Tyagi
The objective of this research was to investigate the kinetics of lipid production by Yarrowia lipolytica SKY7 in the crude glycerol-supplemented media with and without the control of pH. Lipid and citric acid production were improved with the pH control condition. There was no significant difference observed in the biomass concentration with or without the pH control. In the pH-controlled experiments, the biomass and lipid concentration reached 18 and 7.78 g/L, (45.5% w/w), respectively, with lipid yield (Yp/s) of 0...
October 12, 2017: Applied Biochemistry and Biotechnology
Macarena Larroude, Ewelina Celinska, Alexandre Back, Stephan Thomas, Jean-Marc Nicaud, Rodrigo Ledesma-Amaro
The increasing market demands of β-carotene as colorant, antioxidant and vitamin precursor, requires novel biotechnological production platforms. Yarrowia lipolytica, is an industrial organism unable to naturally synthesize carotenoids but with the ability to produce high amounts of the precursor Acetyl-CoA. We first found that a lipid overproducer strain was capable of producing more β-carotene than a wild type after expressing the heterologous pathway. Thereafter, we developed a combinatorial synthetic biology approach base on Golden Gate DNA assembly to screen the optimum promoter-gene pairs for each transcriptional unit expressed...
October 7, 2017: Biotechnology and Bioengineering
Andrea Lubbe, Benjamin P Bowen, Trent Northen
Microbial consortia have the potential to perform complex, industrially important tasks. The design of microbial consortia requires knowledge of the substrate preferences and metabolic outputs of each member, to allow understanding of potential interactions such as competition and beneficial metabolic exchange. Here, we used exometabolite profiling to follow the resource processing by a microbial co-culture of two biotechnologically relevant microbes, the bacterial cellulose degrader Cellulomonas fimi, and the oleaginous yeast Yarrowia lipolytica...
October 4, 2017: Metabolites
Igor G Morgunov, Olga V Karpukhina, Svetlana V Kamzolova, Vladimir A Samoilenko, Anatoly N Inozemtsev
The effect of biologically active form (threo-Ds-) of isocitric acid on oxidative stress was studied using the infusorian Paramecium caudatum stressed by hydrogen peroxide and salts of some heavy metals (Cu, Pb, Zn, and Cd). Isocitric acid at concentrations between 0.5 and 10 mM favorably influenced the infusorian cells with oxidative stress induced by the toxicants studied. The maximal antioxidant effect of isocitric acid was observed at its concentration 10 mM irrespective of the toxicant used (either H2O2 or heavy metal ions)...
October 4, 2017: Preparative Biochemistry & Biotechnology
Dorota A Rzechonek, Cécile Neuvéglise, Hugo Devillers, Waldemar Rymowicz, Aleksandra M Mirończuk
The gene YALI0F01562g was identified as an important factor involved in erythritol catabolism of the unconventional yeast Yarrowia lipolytica. Its putative role was identified for the first time by comparative analysis of four Y. lipolytica strains: A-101.1.31, Wratislavia K1, MK1 and AMM. The presence of a mutation that seriously damaged the gene corresponded to inability of the strain Wratislavia K1 to utilize erythritol. RT-PCR analysis of the strain MK1 demonstrated a significant increase in YALI0F01562g expression during growth on erythritol...
October 2, 2017: Scientific Reports
Ines Lačanin, Jérôme Mounier, Audrey Pawtowski, Marta Dušková, Josef Kameník, Renáta Karpíšková
Fungi are commonly involved in dairy product spoilage and the use of bioprotective cultures can be a complementary approach to reduce food waste and economic losses. In this study, the antifungal activity of 89 Lactobacillus and 23 Pediococcus spp. isolates against three spoilage species, e.g., Yarrowia lipolytica, Rhodotorula mucilaginosa and Penicillium brevicompactum, was first evaluated in milk agar. None of the tested pediococci showed antifungal activity while 3, 23 and 43 lactobacilli isolates showed strong antifungal activity or total inhibition against Y...
September 30, 2017: World Journal of Microbiology & Biotechnology
Pauline Trébulle, Jean-Marc Nicaud, Christophe Leplat, Mohamed Elati
Complex phenotypes, such as lipid accumulation, result from cooperativity between regulators and the integration of multiscale information. However, the elucidation of such regulatory programs by experimental approaches may be challenging, particularly in context-specific conditions. In particular, we know very little about the regulators of lipid accumulation in the oleaginous yeast of industrial interest Yarrowia lipolytica. This lack of knowledge limits the development of this yeast as an industrial platform, due to the time-consuming and costly laboratory efforts required to design strains with the desired phenotypes...
2017: NPJ Systems Biology and Applications
Dorota A Rzechonek, Adam Dobrowolski, Waldemar Rymowicz, Aleksandra M Mirończuk
Erythritol is a natural sweetener commonly used in the food and pharmaceutical industries. Produced by microorganisms as an osmoprotectant, it is an ideal sucrose substitute for diabetics or overweight persons due to its almost zero calorie content. Currently, erythritol is produced on an industrial scale through the fermentation of sugars by some yeasts, such as Moniliella sp. However, the popularity of erythritol as a sweetener is still small because of its high retail price. This creates an opportunity for further process improvement...
September 27, 2017: Critical Reviews in Biotechnology
Chandrika Bendigiri, Smita Zinjarde, Ameeta RaviKumar
Recalcitrant environmental pollutants, like bromoorganics and epoxides are hydrolysed with limited substrate specificities by microbial oxygenases, reductases, hydrolases and dehalogenases. Here, we report the identification and characterisation of a protein (XP_504164) from the tropical marine yeast Yarrowia lipolytica NCIM 3589, known to degrade bromoorganics and epoxides. Multiple sequence alignment suggests it belongs to α/β superfamily with conservation of catalytic triad and oxyanion hole motifs. The corresponding gene cloned and protein (Ylehd) expressed in E...
September 19, 2017: Scientific Reports
Jeffrey Czajka, Qinhong Wang, Yechun Wang, Yinjie J Tang
Genetically modified microbes have had much industrial success producing protein-based products (such as antibodies and enzymes). However, engineering microbial workhorses for biomanufacturing of commodity compounds remains challenging. First, microbes cannot afford burdens with both overexpression of multiple enzymes and metabolite drainage for product synthesis. Second, synthetic circuits and introduced heterologous pathways are not yet as "robust and reliable" as native pathways due to hosts' innate regulations, especially under suboptimal fermentation conditions...
September 7, 2017: Applied Microbiology and Biotechnology
Asma Timoumi, Carine Bideaux, Stéphane E Guillouet, Yohan Allouche, Carole Molina-Jouve, Luc Fillaudeau, Nathalie Gorret
Dynamic behavior of Yarrowia lipolytica W29 strain under conditions of fluctuating, low, and limited oxygen supply was characterized in batch and glucose-limited chemostat cultures. In batch cultures, transient oscillations between oxygen-rich and -deprived environments induced a slight citric acid accumulation (lower than 29 mg L(-1)). By contrast, no citric acid was detected in continuous fermentations for all stress conditions: full anoxia (zero pO2 value, 100% N2), limited (zero pO2 value, 75% of cell needs), and low (pO2 close to 2%) dissolved oxygen (DO) levels...
September 6, 2017: Applied Microbiology and Biotechnology
Dayana Pereira de Andrade, Beatriz Ferreira Carvalho, Rosane Freitas Schwan, Disney Ribeiro Dias
γ-Decalactone is a flavour compound that when obtained by biotechnological production using microorganisms is classified as natural. The aim of this study is to evaluate various conditions for γ-decalactone production by tropical yeast strains Yarrowia lipolytica CCMA 0242 and Lindnera saturnus CCMA 0243. The growth of and γ-decalactone production by Y. lipolytica CCMA 0242 were higher in castor oil than in glycerol. γ-Decalactone production in single batch or fed-batch fermentation did not differ significantly...
June 2017: Food Technology and Biotechnology
Erika Alamillo, Martha Reyes-Becerril, Alberto Cuesta, Carlos Angulo
The climatic conditions in saltern saline environments allows the growth of microorganisms adapted to these peculiar ambient and could represent a promising source of new bioactive compounds that could have applications on as animal food supplements, including aquaculture. In this study, we evaluated the role of Yarrowia lipolytica N-6 isolate, from a hypersaline natural environment (Guerrero Negro, Baja California Sur, Mexico), as immunostimulant of the non-specific immune response of head-kidney and spleen Pacific red snapper (Lutjanus peru) leukocytes after challenge with Vibrio parahaemolyticus...
August 30, 2017: Fish & Shellfish Immunology
Faseela Hamza, Amogh Vaidya, Mugdha Apte, Ameeta Ravi Kumar, Smita Zinjarde
Controlling disease outbreaks is a major challenge in aquaculture farms and conventional methods are often ineffective. Nutritional supplementation and probiotic preparations help in reducing severity of such infections. The generally regarded as safe yeast (Yarrowia lipolytica) was used in the current study. A marine strain of Y. lipolytica exhibited tolerance towards sodium selenite and formed cell associated selenium nanoparticles (SeNPs). The synthesized nanoparticles were characterized by UV-vis spectroscopy, X-ray diffraction (XRD) and Field Emission Scanning Electron Microscope (FE-SEM) observations...
November 2017: Enzyme and Microbial Technology
Michael Dare Asemoloye, Rafiq Ahmad, Segun Gbolagade Jonathan
Fungi are usually involved in degradation/deterioration of many anthropogenic wastes due to their verse enzyme secretions and adaptive capabilities. In this study, five dominant fungal strains were isolated from an aged lindane polluted site, they were all mixed (100 mg each) together with pent mushroom compost (SMC) and applied to lindane polluted soil (5 kg) at 10, 20, 30, 40% and control 0% (soil with no treatment), these were used to grow M. maximus Jacq for 3 months. To establish lindane degradation, deductions such as Degradation rate (K1), Half-life (t1/2) and Degradation efficiency (DE) were made based on the analyzed lindane concentrations before and after the experiment...
2017: PloS One
Xiaoyan Liu, Xinjun Yu, Jun Xia, Jinshun Lv, Jiaxing Xu, Benlin Dai, Xiangqian Xu, Jiming Xu
Okara (soybean residue) is an ideal erythritol feedstock due to its low price and high-nutrient content. Fungal-fermentations were carried out in okara using in-house enzyme pools generated by Mucor flavus or Trichoderma reesei to make okara more accessibility in the subsequent erythritol production using Yarrowia lipolytica. Mucor-fermented okara produced a high erythritol yield because of its special component and micromorphology. Five days is the optimal period for Mucor fermentation. Different fermentation modes were compared in terms of erythritol production and yield...
November 2017: Bioresource Technology
Evgeniya Y Yuzbasheva, Elizaveta B Mostova, Natalia I Andreeva, Tigran V Yuzbashev, Alexander S Fedorov, Irina A Konova, Sergey P Sineoky
In recent years, bio-based production of free fatty acids from renewable resources has attracted attention for their potential as precursors for the production of biofuels and biochemicals. In this study, the oleaginous yeast Yarrowia lipolytica was engineered to produce free fatty acids by eliminating glycerol metabolism. Free fatty acid production was monitored under lipogenic conditions with glycerol as a limiting factor. Firstly, the strain W29 (Δgpd1), which is deficient in glycerol synthesis, was obtained...
August 19, 2017: Biotechnology and Bioengineering
Cory Schwartz, Keith Frogue, Adithya Ramesh, Joshua Misa, Ian Wheeldon
In many organisms of biotechnological importance precise genome editing is limited by inherently low homologous recombination (HR) efficiencies. A number of strategies exist to increase the effectiveness of this native DNA repair pathway; however, most strategies rely on permanently disabling competing repair pathways, thus reducing an organism's capacity to repair naturally occurring double strand breaks. Here, we describe a CRISPR interference (CRISPRi) system for gene repression in the oleochemical-producing yeast Yarrowia lipolytica...
August 19, 2017: Biotechnology and Bioengineering
Lifeng Ping, Xiaoli Yuan, Min Zhang, Yanjun Chai, Shengdao Shan
Aarrowia lipolytica Wt-11 producing an extracellular lipase was isolated and identified. To improve the lipase production, Y. lipolytica Wt-11 was subjected to low-energy ion implantation mutation breeding, and a best mutant, Y. lipolytica Mut-96, was obtained after screening. Under the optimal cultivation conditions, the scaled-up production of lipases were performed, and the lipase activity of Y. lipolytica Mut-96 was enhanced nearly 5.5-fold compared with that of Y. lipolytica Wt-11. After fermentation, the lipases were purified, and the characteristics of the purified lipases were studied...
August 4, 2017: International Journal of Biological Macromolecules
Marion Trassaert, Marie Vandermies, Fréderic Carly, Olivia Denies, Stéphane Thomas, Patrick Fickers, Jean-Marc Nicaud
BACKGROUND: The oleaginous yeast Yarrowia lipolytica is increasingly used as alternative cell factory for the production of recombinant proteins. At present, several promoters with different strengths have been developed based either on the constitutive pTEF promoter or on oleic acid inducible promoters such as pPOX2 and pLIP2. Although these promoters are highly efficient, there is still a lack of versatile inducible promoters for gene expression in Y. lipolytica. RESULTS: We have isolated and characterized the promoter of the EYK1 gene coding for an erythrulose kinase...
August 15, 2017: Microbial Cell Factories
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