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https://www.readbyqxmd.com/read/27875768/high-efficiency-succinic-acid-production-from-glycerol-via-in-situ-fibrous-bed-bioreactor-with-an-engineered-yarrowia-lipolytica
#1
Chong Li, Xiaofeng Yang, Shi Gao, Huaimin Wang, Carol Sze Ki Lin
In this study, in situ fibrous bed bioreactor (isFBB) was developed at the first time for efficient succinic acid (SA) production by Yarrowia lipolytica. After optimization, SA titer, productivity and yield of 51.9g/L, 1.46g/L/h and 0.42g/g were obtained respectively via isFBB fermentation under conditions of 750cm(2) cotton towel, 120g/L initial glycerol and 3L/min aeration rate. By fed batch strategy, SA titer raised up to 198.2g/L was achieved, which was the highest value ever reported. In operation stability study, SA productivity showed no obvious decrease after 12 repeated batches of 460h fermentation, and cell viability even recovered within two repeated batches after intentional interruption...
November 12, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27871794/accessory-subunit-nuym-ndufs4-is-required-for-stability-of-the-electron-input-module-and-activity-of-mitochondrial-complex-i
#2
Flora Kahlhöfer, Katarzyna Kmita, Ilka Wittig, Klaus Zwicker, Volker Zickermann
Mitochondrial complex I is an intricate 1MDa membrane protein complex with a central role in aerobic energy metabolism. The minimal form of complex I consists of fourteen central subunits that are conserved from bacteria to man. In addition, eukaryotic complex I comprises some 30 accessory subunits of largely unknown function. The gene for the accessory NDUFS4 subunit of human complex I is a hot spot for fatal pathogenic mutations in humans. We have deleted the gene for the orthologous NUYM subunit in the aerobic yeast Yarrowia lipolytica, an established model system to study eukaryotic complex I and complex I linked diseases...
November 18, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27871377/enhancement-of-methanol-resistance-of-yarrowia-lipolytica-lipase-2-using-%C3%AE-cyclodextrin-as-an-additive-insights-from-experiments-and-molecular-dynamics-simulation
#3
Hao Cao, Yang Jiang, Haiyang Zhang, Kaili Nie, Ming Lei, Li Deng, Fang Wang, Tianwei Tan
The methanol resistance of lipase is a critical parameter in enzymatic biodiesel production. In the present work, the methanol resistance of Yarrowia lipolytica Lipase 2 (YLLIP2) was significantly improved using β-cyclodextrin (β-CD) as an additive. According to the results, YLLIP2 with β-CD exhibited approximately 7000U/mg specific activity in 30wt% methanol for 60min compared with no activity without β-CD under the same conditions. Molecular dynamics (MD) simulation results indicated that the β-CD molecules weakened the conformational change of YLLIP2 and maintained a semi-open state of the lid by overcoming the interference caused by methanol molecules...
January 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/27861744/omega-3-production-by-fermentation-of-yarrowia-lipolytica-from-fed-batch-to-continuous
#4
Dongming Xie, Edward Miller, Pamela Sharpe, Ethel Jackson, Quinn Zhu
The omega-3 fatty acid, cis-5,8,11,14,17-eicosapentaenoic acid (C20:5; EPA) has wide-ranging benefits in improving heart health, immune function, and mental health. A sustainable source of EPA production through fermentation of metabolically engineered Yarrowia lipolytica has been developed. In this paper, key fed-batch fermentation conditions were identified to achieve 25% EPA in the yeast biomass, which is so far the highest EPA titer reported in the literature. Dynamic models of the EPA fermentation process were established for analyzing, optimizing, and scaling up the fermentation process...
November 14, 2016: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/27831926/biodegradation-of-micropollutant-naproxen-with-a-selected-fungal-strain-and-identification-of-metabolites
#5
Y Doruk Aracagök, Hakan Göker, Nilüfer Cihangir
Pharmaceuticals are widely used for treating human and animal diseases. Naproxen [(S) 6-methoxy-α-methyl-2-naphthalene acetic acid] and its sodium salt are members of the α-arylpropionic acid group of nonsteroidal anti-inflammatory drugs. Due to excessive usage of naproxen, this drug has been determined even in drinking water. In this study, four fungal strains Phanerochaete chrysosporium, Funalia trogii, Aspergillus niger, and Yarrowia lipolytica were investigated in terms of naproxen removal abilities. According to LC/MS data, A...
November 10, 2016: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/27826725/engineering-yarrowia-lipolytica-for-poly-3-hydroxybutyrate-production
#6
Zheng-Jun Li, Kangjian Qiao, Nian Liu, Gregory Stephanopoulos
Strains of Yarrowia lipolytica were engineered to express the poly-3-hydroxybutyrate (PHB) biosynthetic pathway. The genes for β-ketothiolase, NADPH-dependent acetoacetyl-CoA reductase, and PHB synthase were cloned and inserted into the chromosome of Y. lipolytica. In shake flasks, the engineered strain accumulated PHB to 1.50 and 3.84% of cell dry weight in complex medium supplemented with glucose and acetate as carbon source, respectively. In fed-batch fermentation using acetate as sole carbon source, 7...
November 8, 2016: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27817013/novel-efficient-promoter-of-the-mitochondrial-porin-voltage-dependent-anion-channel-vdac-in-the-genome-of-the-yarrowia-lipolytica-yeast
#7
F F Kulanbaewa, V Yu Sekova, E P Isakova, Y I Deryabina, A V Nikolaev
This article presents the characteristics of the highly inducible promoter of the gene encoding the mitochondrial porin, the voltage-dependent anion channel (VDAC). This promoter is recommended for use in new genetic constructs both in basic research for assessing the adaptive strategy of lower eukaryotes under adverse conditions and in designing new highly competitive transformants producing economically important compounds (proteins, lipids, and organic acids) on its basis.
September 2016: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27809258/biotransformation-of-bicyclic-halolactones-with-a-methyl-group-in-the-cyclohexane-ring-into-hydroxylactones-and-their-biological-activity
#8
Katarzyna Wińska, Małgorzata Grabarczyk, Wanda Mączka, Barbara Żarowska, Gabriela Maciejewska, Katarzyna Dancewicz, Beata Gabryś, Antoni Szumny, Mirosław Anioł
The aim of this study was the chemical synthesis of a series of halo- and unsaturated lactones, as well as their microbial transformation products. Finally some of their biological activities were assessed. Three bicyclic halolactones with a methyl group in the cyclohexane ring were obtained from the corresponding γ,δ-unsaturated ester during a two-step synthesis. These lactones were subjected to screening biotransformation using twenty two fungal strains. These strains were tested on their ability to transform halolactones into new hydroxylactones...
October 31, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27800013/fatty-alcohol-production-in-lipomyces-starkeyi-and-yarrowia-lipolytica
#9
Wei Wang, Hui Wei, Eric Knoshaug, Stefanie Van Wychen, Qi Xu, Michael E Himmel, Min Zhang
BACKGROUND: Current biological pathways to produce biofuel intermediates amenable to separations and catalytic upgrading to hydrocarbon fuels are not cost effective. Previously, oleaginous yeasts have been investigated primarily for lipid production. However, yeasts store neutral lipids intracellularly making recovery difficult and expensive. In addition, once recovered from the cells, lipids are difficult to blend directly with the existing fuels without upgrading. We have, therefore, begun to investigate secreted fatty acid-derived products which can be easily recovered and upgraded to fuels...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27796439/applying-pathway-engineering-to-enhance-production-of-alpha-ketoglutarate-in-yarrowia-lipolytica
#10
REVIEW
Hongwei Guo, Shaojie Su, Catherine Madzak, Jingwen Zhou, Hongwen Chen, Guo Chen
α-Ketoglutarate (α-KG), one of short-chain carboxylates of high commercial relevance, has been widely used in food, medicine, chemical, and cosmetic fields. Compared to other carboxylates, α-KG occupies key positions in the tricarboxylate cycle (TCA cycle) and amino acid metabolic pathway, the over-accumulation of α-KG is restricted both by tighter carbon and nitrogen regulation process. Biotechnology production of α-KG on large industrial level has been impeded by many obstacles. This review aims at highlighting and stating recent efforts toward improving the yield and titer of α-KG in the strains of Yarrowia lipolytica to reach industrial relevance...
October 29, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27795258/draft-genome-sequence-of-yarrowia-lipolytica-strain-a-101-isolated-from-polluted-soil-in-poland
#11
Hugo Devillers, François Brunel, Xymena Połomska, Véronique Sarilar, Zbigniew Lazar, Małgorzata Robak, Cécile Neuvéglise
Yarrowia lipolytica is an early diverging species of the Saccharomycotina subphylum, which is recognized as a valuable host for many biotechnological applications exploiting its oleaginous capacities. The 20.5-Mb genome of the Polish Y. lipolytica strain A-101 will greatly help decipher the genetic basis of the regulation of its lipid metabolism.
October 6, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27794247/diversity-of-spoilage-fungi-associated-with-various-french-dairy-products
#12
Lucille Garnier, Florence Valence, Audrey Pawtowski, Lizaveta Auhustsinava-Galerne, Nicolas Frotté, Riccardo Baroncelli, Franck Deniel, Emmanuel Coton, Jérôme Mounier
Yeasts and molds are responsible for dairy product spoilage, resulting in significant food waste and economic losses. Yet, few studies have investigated the diversity of spoilage fungi encountered in dairy products. In the present study, 175 isolates corresponding to 105 from various spoiled dairy products and 70 originating from dairy production environments, were identified using sequencing of the ITS region, the partial β-tubulin, calmodulin and/or EFα genes, and the D1-D2 domain of the 26S rRNA gene for filamentous fungi and yeasts, respectively...
October 21, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27790411/protective-yeasts-control-v-anguillarum-pathogenicity-and-modulate-the-innate-immune-response-of-challenged-zebrafish-danio-rerio-larvae
#13
Mario Caruffo, Natalie C Navarrete, Oscar A Salgado, Nelly B Faúndez, Miguel C Gajardo, Carmen G Feijóo, Angélica Reyes-Jara, Katherine García, Paola Navarrete
We investigated mechanisms involved in the protection of zebrafish (Danio rerio) larvae by two probiotic candidate yeasts, Debaryomyces hansenii 97 (Dh97) and Yarrowia lypolitica 242 (Yl242), against a Vibrio anguillarum challenge. We determined the effect of different yeast concentrations (10(4)-10(7) CFU/mL) to: (i) protect larvae from the challenge, (ii) reduce the in vivo pathogen concentration and (iii) modulate the innate immune response of the host. To evaluate the role of zebrafish microbiota in protection, the experiments were performed in conventionally raised and germ-free larvae...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/27780240/expression-of-soluble-forms-of-yeast-diacylglycerol-acyltransferase-2-that-integrate-a-broad-range-of-saturated-fatty-acids-in-triacylglycerols
#14
Nawel Haïli, Julien Louap, Michel Canonge, Franjo Jagic, Christelle Louis-Mondésir, Thierry Chardot, Pierre Briozzo
The membrane proteins acyl-CoA:diacylglycerol acyltransferases (DGAT) are essential actors for triglycerides (TG) biosynthesis in eukaryotic organisms. Microbial production of TG is of interest for producing biofuel and value-added novel oils. In the oleaginous yeast Yarrowia lipolytica, Dga1p enzyme from the DGAT2 family plays a major role in TG biosynthesis. Producing recombinant DGAT enzymes pure and catalytically active is difficult, hampering their detailed functional characterization. In this report, we expressed in Escherichia coli and purified two soluble and active forms of Y...
2016: PloS One
https://www.readbyqxmd.com/read/27777617/metabolic-engineering-of-oleaginous-yeast-yarrowia-lipolytica-for-limonene-overproduction
#15
Xuan Cao, Yu-Bei Lv, Jun Chen, Tadayuki Imanaka, Liu-Jing Wei, Qiang Hua
BACKGROUND: Limonene, a monocyclic monoterpene, is known for its using as an important precursor of many flavoring, pharmaceutical, and biodiesel products. Currently, d-limonene has been produced via fractionation from essential oils or as a byproduct of orange juice production, however, considering the increasing need for limonene and a certain amount of pesticides may exist in the limonene obtained from the citrus industry, some other methods should be explored to produce limonene. RESULTS: To construct the limonene synthetic pathway in Yarrowia lipolytica, two genes encoding neryl diphosphate synthase 1 (NDPS1) and limonene synthase (LS) were codon-optimized and heterologously expressed in Y...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27775845/heterologous-expression-of-lipases-ylip4-ylip5-ylip7-ylip13-and-ylip15-from-yarrowia-lipolytica-msr80-in-e-coli-substrate-specificity-kinetic-comparison-and-enantioselectivity
#16
Poonam Syal, Rani Gupta
Five lipase genes, ylip4, ylip5, ylip7, ylip13 and ylip15, from Yarrowia lipolytica MSR80 were cloned and expressed in pEZZ18-HB101 system. The lipases shared maximum sequence identity with Candida galli lipase, while they shared structural similarity with YLIP2 of Y. lipolytica CLIB122. The enzymes, purified using IgG Sepharose, had specific activities in the range of 7-25 U/mg. Biochemical characteristics of all the lipases varied with respect to thermostability, substrate specificity and enantioselectivity...
October 24, 2016: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/27765697/comparative-study-of-two-single-crd-c-type-lectins-cgclec-4-and-cgclec-5-from-pacific-oyster-crassostrea-gigas
#17
Zhihao Jia, Huan Zhang, Shuai Jiang, Mengqiang Wang, Lingling Wang, Linsheng Song
C-type lectins (CTLs), a superfamily of Ca(2+)-dependent carbohydrate-recognition proteins, are involved in nonself-recognition and pathogen elimination, and play crucial roles in the innate immunity. In the present study, two single CRD C-type lectins, CgCLec-4 and CgCLec-5, were identified from oyster Crassostrea gigas. The open reading frame (ORF) of CgCLec-4 and CgCLec-5 encoded polypeptides of 152 and 150 amino acids, respectively. Both CgCLec-4 and CgCLec-5 contained one CRD with six conserved cysteines to form three disulfide bridges...
December 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27761435/-13-c-metabolic-flux-analysis-for-systematic-metabolic-engineering-of-s-cerevisiae-for-overproduction-of-fatty-acids
#18
Amit Ghosh, David Ando, Jennifer Gin, Weerawat Runguphan, Charles Denby, George Wang, Edward E K Baidoo, Chris Shymansky, Jay D Keasling, Héctor García Martín
Efficient redirection of microbial metabolism into the abundant production of desired bioproducts remains non-trivial. Here, we used flux-based modeling approaches to improve yields of fatty acids in Saccharomyces cerevisiae. We combined (13)C labeling data with comprehensive genome-scale models to shed light onto microbial metabolism and improve metabolic engineering efforts. We concentrated on studying the balance of acetyl-CoA, a precursor metabolite for the biosynthesis of fatty acids. A genome-wide acetyl-CoA balance study showed ATP citrate lyase from Yarrowia lipolytica as a robust source of cytoplasmic acetyl-CoA and malate synthase as a desirable target for downregulation in terms of acetyl-CoA consumption...
2016: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/27744524/production-of-laccase-by-recombinant-yarrowia-lipolytica-from-molasses-bioprocess-development-using-statistical-modeling-and-increase-productivity-in-shake-flask-and-bioreactor-cultures
#19
Farshad Darvishi, Marzieh Moradi, Catherine Madzak, Claude Jolivalt
Laccases are used in numerous applications, from green degradation of various xenobiotic compounds, waste detoxification, textile dye bleaching, and delignification of lignocellulose materials to biofuel production. In this study, the recombinant Yarrowia lipolytica YL4 strain carrying the white-rot fungus Trametes versicolor laccase IIIb gene was used for laccase production from beet molasses as an agro-industrial residue. Response surface methodology was used to statistical optimization of the production of laccase by Y...
October 15, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27743491/characterization-of-phosphatidic-acid-phosphatase-activity-in-the-oleaginous-yeast-yarrowia-lipolytica-and-its-role-in-lipid-biosynthesis
#20
Derell Hardman, Daniel McFalls, Stylianos Fakas
Phosphatidic acid phosphatase (PAP) catalyses the committed step of triacylglycerol (TAG) biosynthesis and thus regulates the amounts of TAG produced by the cell. TAG is the target of biotechnological processes developed for the production of food lipids or biofuels. These processes are using oleaginous microorganisms like the yeast Yarrowia lipolytica as the TAG producers. Thus manipulating key enzymatic activities like PAP in Y. lipolytica could drive lipid biosynthesis towards TAG production and increase TAG yields...
October 15, 2016: Yeast
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