keyword
MENU ▼
Read by QxMD icon Read
search

yarrowia

keyword
https://www.readbyqxmd.com/read/28634240/leucine-biosynthesis-is-involved-in-regulating-high-lipid-accumulation-in-yarrowia-lipolytica
#1
Eduard J Kerkhoven, Young-Mo Kim, Siwei Wei, Carrie D Nicora, Thomas L Fillmore, Samuel O Purvine, Bobbie-Jo Webb-Robertson, Richard D Smith, Scott E Baker, Thomas O Metz, Jens Nielsen
The yeast Yarrowia lipolytica is a potent accumulator of lipids, and lipogenesis in this organism can be influenced by a variety of factors, such as genetics and environmental conditions. Using a multifactorial study, we elucidated the effects of both genetic and environmental factors on regulation of lipogenesis in Y. lipolytica and identified how two opposite regulatory states both result in lipid accumulation. This study involved comparison of a strain overexpressing diacylglycerol acyltransferase (DGA1) with a control strain grown under either nitrogen or carbon limitation conditions...
June 20, 2017: MBio
https://www.readbyqxmd.com/read/28631058/expression-and-characterization-of-glucose-oxidase-from-aspergillus-niger-in-yarrowia-lipolytica
#2
Fatemeh Khadivi Derakshan, Farshad Darvishi, Mehrouz Dezfulian, Catherine Madzak
Glucose oxidase (GOX) is currently used in clinical, pharmaceutical, food and chemical industries. The aim of this study was expression and characterization of Aspergillus niger glucose oxidase gene in the yeast Yarrowia lipolytica. For the first time, the GOX gene of A. niger was successfully expressed in Y. lipolytica using a mono-integrative vector containing strong hybrid promoter and secretion signal. The highest total glucose oxidase activity was 370 U/L after 7 days of cultivation. An innovative method was used to cell wall disruption in current study, and it could be recommended to use for efficiently cell wall disruption of Y...
June 19, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/28630295/application-of-metabolic-controls-for-the-maximization-of-lipid-production-in-semicontinuous-fermentation
#3
Jingyang Xu, Nian Liu, Kangjian Qiao, Sebastian Vogg, Gregory Stephanopoulos
Acetic acid can be generated through syngas fermentation, lignocellulosic biomass degradation, and organic waste anaerobic digestion. Microbial conversion of acetate into triacylglycerols for biofuel production has many advantages, including low-cost or even negative-cost feedstock and environmental benefits. The main issue stems from the dilute nature of acetate produced in such systems, which is costly to be processed on an industrial scale. To tackle this problem, we established an efficient bioprocess for converting dilute acetate into lipids, using the oleaginous yeast Yarrowia lipolytica in a semicontinuous system...
June 19, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28627452/engineering-of-unconventional-yeast-yarrowia-lipolytica-for-efficient-succinic-acid-production-from-glycerol-at-low-ph
#4
Zhiyong Cui, Cuijuan Gao, Jiaojiao Li, Jin Hou, Carol Sze Ki Lin, Qingsheng Qi
Yarrowia lipolytica is considered as a potential candidate for succinic acid production because of its innate ability to accumulate citric acid cycle intermediates and its tolerance to acidic pH. Previously, a succinate-production strain was obtained through the deletion of succinate dehydrogenase subunit encoding gene Ylsdh5. However, the accumulation of by-product acetate limited further improvement of succinate production. Meanwhile, additional pH adjustment procedure increased the downstream cost in industrial application...
June 13, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28608278/identification-and-characterization-of-eyk1-a-key-gene-for-erythritol-catabolism-in-yarrowia-lipolytica
#5
F Carly, H Gamboa-Melendez, M Vandermies, C Damblon, J M Nicaud, P Fickers
Erythritol is a four-carbon sugar alcohol synthesized by osmophilic yeasts, such as Yarrowia lipolytica, in response to osmotic stress. This metabolite has application as food additive due to its sweetening properties. Although Y. lipolytica can produce erythritol at a high level from glycerol, it is also able to consume it as carbon source. This ability negatively affects erythritol productivity and represents a serious drawback for the development of an efficient erythritol production process. In this study, we have isolated by insertion mutagenesis a Y...
June 12, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28591644/co-expression-of-glucose-6-phosphate-dehydrogenase-and-acyl-coa-binding-protein-enhances-lipid-accumulation-in-the-yeast-yarrowia-lipolytica
#6
Evgeniya Y Yuzbasheva, Elizaveta B Mostova, Natalia I Andreeva, Tigran V Yuzbashev, Ivan A Laptev, Tatiana I Sobolevskaya, Sergey P Sineoky
The oleaginous yeast Yarrowia lipolytica is a convenient model for investigating lipid biosynthesis and for engineering high lipid accumulated strains. In this organism, the pentose phosphate pathway is the major source of NADPH for lipid biosynthesis. Thus, we over-expressed gene encoding NADP(+)-dependent glucose-6-phosphate dehydrogenase (ZWF1) in a strain deficient in peroxisome biogenesis. However, this strategy suppressed growth during cultivation under lipogenic conditions and did not significantly increase lipid accumulation...
June 4, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28589225/characterization-of-the-newly-isolated-%C3%AF-oxidizing-yeast-candida-sorbophila-ds02-and-its-potential-applications-in-long-chain-dicarboxylic-acid-production
#7
Heeseok Lee, Yohanes Eko Chandra Sugiharto, Seunghoon Lee, Gyuyeon Park, Changpyo Han, Hyeran Jang, Wooyoung Jeon, Heejoon Park, Jungoh Ahn, Kyungbo Kang, Hongwoen Lee
α, ω-Dicarboxylic acids (DCAs) are multipurpose chemicals widely used in polymers, perfumes, plasticizers, lubricants, and adhesives. The biotransformation of DCAs from alkanes and fatty acids by microorganisms has attracted recent interest, since synthesis via chemical oxidation causes problems in terms of the environment and safety. We isolated an ω-oxidizing yeast from a wastewater disposal facility of a petrochemical factory by chemostat enrichment culture. The haploid strain identified as Candida sorbophila DS02 grew on glucose and dodecane, exhibiting greater cell shrinkage on the latter...
June 6, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28585817/production-of-medium-chain-fatty-acids-by-yarrowia-lipolytica-combining-molecular-design-and-talen-to-engineer-the-fatty-acid-synthase
#8
Coraline Rigouin, Marc Guéroult, Christian Croux, Gwendoline Dubois, Vinciane Borsenberger, Sophie Barbe, Alain Marty, Fayza Daboussi, Isabelle André, Florence Bordes
Yarrowia lipolytica is a promising organism for the production of lipids of biotechnological interest and particularly for biofuel. In this study, we engineered the key enzyme involved in lipid biosynthesis, the giant multifunctional Fatty Acid Synthase (FAS), to shorten chain length of the synthesized fatty acids. Taking as starting point that the Ketoacyl Synthase (KS) domain of Yarrowia lipolytica FAS is directly involved in chain length specificity, we used molecular modelling to investigate molecular recognition of palmitic acid (C16 fatty acid) by the KS...
June 6, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28583872/targeted-mutations-and-md-simulations-of-a-methanol-stable-lipase-ylip9-from-yarrowia-lipolytica-msr80-to-develop-a-biodiesel-enzyme
#9
Poonam Syal, Ved Vrat Verma, Rani Gupta
Biodiesel, an environment friendly alternative for fuels, contains methyl esters of long-chain fatty acids. Our group has reported a methanol-stable YLIP9 from Yarrowia lipolytica MSR80 that shows poor catalysis of long-chain fatty acids. To shift its substrate specificity, residues within lid and binding pocket were identified for sequential mutations using YLIP2 as the template. Of the two point mutations (Glu116Leu and Ser119Val) introduced in the lid, the former mutation (YLIP9L1) increased the catalytic rate by ∼2-fold without any change in substrate specificity...
June 2, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28559741/an-effective-method-of-continuous-production-of-erythritol-from-glycerol-by-yarrowia-lipolytica-mk1
#10
Magdalena Rakicka, Aleksandra M Mirończuk, Ludwika Tomaszewska-Hetman, Anita Rywińska, Waldemar Rymowicz
This study demonstrates the potential applicability of the UV mutant Yarrowia lipolytica MK1 for the valorisation of glycerol and erythritol production in a chemostat culture. The aim of this research is to investigate the optimal C:N ratio in the feeding medium in order to enhance erythritol production. The highest erythritol concentration, at 113.1 g/L with a volumetric erythritol production rate of 1.1 g/(L·h) and a yield of 0.57 g/g, was obtained in the feeding medium with a C:N ratio of 80:1. Moreover, no residual glycerol was observed in the culture broth during cultivation...
March 2017: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/28552427/eyk1-encoding-erythrulose-kinase-as-a-catabolic-selectable-marker-for-genome-editing-in-the-non-conventional-yeast-yarrowia-lipolytica
#11
Marie Vandermies, Olivia Denies, Jean-Marc Nicaud, Patrick Fickers
We report here on EYK1, encoding erythrulose kinase, as an efficient catabolic selectable marker for genome editing in Y. lipolytica. Compared to auxotrophic markers, EYK1 increases the growth rate of transformants and allows improved efficiency of transformation. The utility of the marker EYK1 in a replicative vector was also demonstrated.
May 26, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28545807/enhancing-erythritol-productivity-in-yarrowia-lipolytica-using-metabolic-engineering
#12
Frédéric Carly, Marie Vandermies, Samuel Telek, Sébastien Steels, Stéphane Thomas, Jean-Marc Nicaud, Patrick Fickers
Erythritol (1,2,3,4-butanetetrol) is a four-carbon sugar alcohol with sweetening properties that is used by the agrofood industry as a food additive. In this study, we demonstrated that metabolic engineering can be used to improve the production of erythritol from glycerol in the yeast Yarrowia lipolytica. The best results were obtained using a mutant that overexpressed GUT1 and TKL1, which encode a glycerol kinase and a transketolase, respectively, and in which EYK1, which encodes erythrulose kinase, was disrupted; the latter enzyme is involved in an early step of erythritol catabolism...
May 22, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28533816/conferring-cellulose-degrading-ability-to-yarrowia-lipolytica-to-facilitate-a-consolidated-bioprocessing-approach
#13
Zhong-Peng Guo, Sophie Duquesne, Sophie Bozonnet, Gianluca Cioci, Jean-Marc Nicaud, Alain Marty, Michael Joseph O'Donohue
BACKGROUND: Yarrowia lipolytica, one of the most widely studied "nonconventional" oleaginous yeast species, is unable to grow on cellulose. Recently, we identified and overexpressed two endogenous β-glucosidases in Y. lipolytica, thus enabling this yeast to use cello-oligosaccharides as a carbon source for growth. Using this engineered yeast platform, we have now gone further toward building a fully cellulolytic Y. lipolytica for use in consolidated bioprocessing of cellulose. RESULTS: Initially, different essential enzyme components of a cellulase cocktail (i...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28528384/effects-of-salts-contained-in-lignocellulose-derived-sugar-streams-on-microbial-lipid-production
#14
Zhu Chen, Caixia Wan
This study aimed at developing low-cost, robust non-sterile fermentation processes for microbial lipid production from lignocellulose-derived sugars. Three representative oleaginous yeasts, Lipomyces tetrasporus (NRRL Y-11562), Rhodotorula toruloides (NRRL Y-1091), and Yarrowia lipolytica (NRRL YB-437), were tested for lipid production via non-sterile fermentation. Under optimal non-sterile conditions, all the tested strains had good performance on salt tolerance and lipid production. L. tetrasporus (NRRL Y-11562) gave the highest lipid titer of 12...
May 20, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28505129/assessment-of-antimicrobial-and-antioxidant-activities-of-nepeta-trachonitica-analysis-of-its-phenolic-compounds-using-hplc-ms-ms
#15
Ekrem Köksal, Hatice Tohma, Ömer Kılıç, Yusuf Alan, Abdülmelik Aras, İlhami Gülçin, Ercan Bursal
Continuing our work on the sources of natural bioactive compounds, we evaluated the antimicrobial and antioxidant activities of Nepeta trachonitica as well as its major phenolic content using the high-performance liquid chromatography-mass spectrometry/mass spectrometry (HPLC-MS/MS) technique. For antioxidant activity, ferric reducing antioxidant power (FRAP) and cupric ion reducing antioxidant capacity (CUPRAC) methods were performed to measure the reducing power and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay was employed to evaluate the radical scavenging activity of the sample...
May 15, 2017: Scientia Pharmaceutica
https://www.readbyqxmd.com/read/28497289/systematic-analysis-of-the-lysine-acetylome-reveals-diverse-functions-of-lysine-acetylation-in-the-oleaginous-yeast-yarrowia-lipolytica
#16
Guangyuan Wang, Lizhong Guo, Wenxing Liang, Zhenming Chi, Lin Liu
Lysine acetylation of proteins, a major post-translational modification, plays a critical regulatory role in almost every aspects in both eukaryotes and prokaryotes. Yarrowia lipolytica, an oleaginous yeast, is considered as a model for bio-oil production due to its ability to accumulate a large amount of lipids. However, the function of lysine acetylation in this organism is elusive. Here, we performed a global acetylproteome analysis of Y. lipolytica ACA-DC 50109. In total, 3163 lysine acetylation sites were identified in 1428 proteins, which account for 22...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28485370/the-digestive-and-defensive-basis-of-carcass-utilization-by-the-burying-beetle-and-its-microbiota
#17
Heiko Vogel, Shantanu P Shukla, Tobias Engl, Benjamin Weiss, Rainer Fischer, Sandra Steiger, David G Heckel, Martin Kaltenpoth, Andreas Vilcinskas
Insects that use ephemeral resources must rapidly digest nutrients and simultaneously protect them from competitors. Here we use burying beetles (Nicrophorus vespilloides), which feed their offspring on vertebrate carrion, to investigate the digestive and defensive basis of carrion utilization. We characterize gene expression and microbiota composition in the gut, anal secretions, and on carcasses used by the beetles. We find a strict functional compartmentalization of the gut involving differential expression of immune effectors (antimicrobial peptides and lysozymes), as well as digestive and detoxifying enzymes...
May 9, 2017: Nature Communications
https://www.readbyqxmd.com/read/28483524/%C3%AE-12-fatty-acid-desaturase-is-involved-in-growth-at-low-temperature-in-yeast-yarrowia-lipolytica
#18
Satoshi Tezaki, Ryo Iwama, Satoshi Kobayashi, Yuh Shiwa, Hirofumi Yoshikawa, Akinori Ohta, Hiroyuki Horiuchi, Ryouichi Fukuda
We investigated the role of FAD2, which was predicted to encode a fatty acid desaturase of the n-alkane-assimilating yeast Yarrowia lipolytica. Northern blot analysis suggested that FAD2 transcription was upregulated at low temperature or in the presence of n-alkanes or oleic acid. The FAD2 deletion mutant grew as well as the wild-type strain on glucose, n-alkanes, or oleic acid at 30 °C, but grew at a slower rate at 12 °C, when compared to the wild-type strain. The growth of the FAD2 deletion mutant at 12 °C was restored by the addition of 18:2, but not 18:1, fatty acids...
June 17, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28482902/substrates-and-oxygen-dependent-citric-acid-production-by-yarrowia-lipolytica-insights-through-transcriptome-and-fluxome-analyses
#19
Wael Sabra, Rajesh Reddy Bommareddy, Garima Maheshwari, Seraphim Papanikolaou, An-Ping Zeng
BACKGROUND: Unlike the well-studied backer yeast where catabolite repression represents a burden for mixed substrate fermentation, Yarrowia lipolytica, an oleaginous yeast, is recognized for its potential to produce single cell oils and citric acid from different feedstocks. These versatilities of Y. lipolytica with regards to substrate utilization make it an attractive host for biorefinery application. However, to develop a commercial process for the production of citric acid by Y. lipolytica, it is necessary to better understand the primary metabolism and its regulation, especially for growth on mixed substrate...
May 8, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28479605/effect-of-oils-extracted-from-plant-seeds-on-the-growth-and-lipolytic-activity-of-yarrowia-lipolytica-yeast
#20
Jolanta Krzyczkowska, Mariola Kozłowska
This study was aimed at evaluating the capability of Yarrowia lipolytica W29 for the synthesis of lipolytic enzymes in a medium containing plant oils from non-conventional sources with some components displaying bioactivity. Oils from almond, hazelnut, and coriander seeds were obtained by using n-hexane (Soxhlet method) and a chloroform/methanol mixture of solvents (Folch method), and their effect on the growth and lipolytic activity of Y. lipolytica was compared. A comparison of these two extraction methods showed that the extraction with n-hexane was less effective regarding the oil extraction yields than the extraction conducted according to Folch's procedure...
2017: Journal of the American Oil Chemists' Society
keyword
keyword
119245
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"