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https://www.readbyqxmd.com/read/28449118/polyphosphate-accumulating-bacterial-community-colonizing-the-calcium-bodies-of-terrestrial-isopod-crustaceans-titanethes-albus-and-hyloniscus-riparius
#1
Rok Kostanjšek, Vittori Miloš, Vesna Srot, Peter A van Aken, Štrus Jasna
Terrestrial isopods from the group Trichoniscidae accumulate calcium in specialized organs, known as the calcium bodies. These consist of two pairs of epithelial sacs located alongside the digestive system. These organs contain various forms of calcium and constantly present bacteria. To elucidate their origin and role, we analyzed the bacteria of the calcium bodies in the cave-dwelling isopod Titanethes albus and the epigean species Hyloniscus riparius, by microscopy, histochemistry, energy dispersive X-ray spectrometry, 16S rRNA analysis and in situ hybridization...
April 25, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28449074/fireprot-web-server-for-automated-design-of-thermostable-proteins
#2
Milos Musil, Jan Stourac, Jaroslav Bendl, Jan Brezovsky, Zbynek Prokop, Jaroslav Zendulka, Tomas Martinek, David Bednar, Jiri Damborsky
There is a continuous interest in increasing proteins stability to enhance their usability in numerous biomedical and biotechnological applications. A number of in silico tools for the prediction of the effect of mutations on protein stability have been developed recently. However, only single-point mutations with a small effect on protein stability are typically predicted with the existing tools and have to be followed by laborious protein expression, purification, and characterization. Here, we present FireProt, a web server for the automated design of multiple-point thermostable mutant proteins that combines structural and evolutionary information in its calculation core...
April 26, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28448830/biogenesis-of-secondary-mycogenic-minerals-related-to-wall-paintings-deterioration-process
#3
Nikola Unković, Suzana Erić, Kristina Šarić, Miloš Stupar, Željko Savković, Slaviša Stanković, Olja Stanojević, Ivica Dimkić, Jelena Vukojević, Milica Ljaljević Grbić
Present study addresses potential of fungal strains, isolated from deteriorated mural paintings and surrounding air environment of the Church of the Holy Ascension in Veliki Krčimir (Serbia), to precipitate mycogenic minerals, when cultivated on agarized B4 medium. Utilizing culture-based isolation methods, 38 filamentous fungi were obtained in total, 23 from mural paintings and 15 from air, respectively, mainly ascomycetes, while Bjerkandera adusta and Thanatephorus cucumeris were only basidiomycetes. A total of 31 of 38 fungal isolates, more than 80%, were able to form minerals of different morphologies and variable size, determined via SEM-EDS and XRPD, to be either calcite or calcite and weddellite association...
April 19, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28446177/the-susceptibility-to-fosfomycin-of-gram-negative-bacteria-isolates-from-urinary-tract-infection-in-the-czech-republic-data-from-a-unicentric-study
#4
Miroslav Fajfr, Miroslav Louda, Pavla Paterová, Lenka Ryšková, Jaroslav Pacovský, Josef Košina, Helena Žemličková, Miloš Broďák
BACKGROUND: Against a background of rapid increase of β-lactamase-producing or multi-resistant pathogenic bacteria and the resulting lack of effective antibiotic treatment, some older antibiotics have been tested for new therapeutic uses. One of these is fosfomycin, to which according to studies these resistant bacteria are very sensitive. Our study was designed because there is no data on the fosfomycin susceptibility rate in the Czech Republic. METHOD: In this study from January 2013 to June 2014 3295 unique isolates of Gram-negative bacteria which had caused urinary tract infections were examined...
April 26, 2017: BMC Urology
https://www.readbyqxmd.com/read/28445767/the-visualized-macrocycles-chemistry-and-application-of-fluorophore-tagged-cyclodextrins
#5
Gábor Benkovics, Milo Malanga, Éva Fenyvesi
Cyclodextrins are macrocyclic molecules able to form host-guest complexes due to their hydrophobic cavity. Because of their carbohydrate nature they do not absorb light in the UV-vis region (200-800nm), but they can be converted into spectroscopically active compounds via modification with a chromophore unit. Among the chromophores, the group of fluorophores can provide high sensitivity in analytical applications (chemosensing) and low detection limit in optical imaging methods (fluorescent microscopy). Fluorophore-tagged cyclodextrins therefore combine interesting spectroscopic properties with promising supramolecular features which make these conjugates widely applicable in various pharmaceutical fields...
April 23, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28429797/sirtuins-in-the-phylum-basidiomycota-a-role-in-virulence-in-cryptococcus-neoformans
#6
Samantha D M Arras, Jessica L Chitty, Maha S I Wizrah, Paige E Erpf, Benjamin L Schulz, Milos Tanurdzic, James A Fraser
Virulence of Cryptococcus neoformans is regulated by a range of transcription factors, and is also influenced by the acquisition of adaptive mutations during infection. Beyond the temporal regulation of virulence factor production by transcription factors and these permanent microevolutionary changes, heritable epigenetic modifications such as histone deacetylation may also play a role during infection. Here we describe the first comprehensive analysis of the sirtuin class of NAD+ dependent histone deacetylases in the phylum Basidiomycota, identifying five sirtuins encoded in the C...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28428560/combination-of-high-density-microelectrode-array-and-patch-clamp-recordings-to-enable-studies-of-multisynaptic-integration
#7
David Jäckel, Douglas J Bakkum, Thomas L Russell, Jan Müller, Milos Radivojevic, Urs Frey, Felix Franke, Andreas Hierlemann
We present a novel, all-electric approach to record and to precisely control the activity of tens of individual presynaptic neurons. The method allows for parallel mapping of the efficacy of multiple synapses and of the resulting dynamics of postsynaptic neurons in a cortical culture. For the measurements, we combine an extracellular high-density microelectrode array, featuring 11'000 electrodes for extracellular recording and stimulation, with intracellular patch-clamp recording. We are able to identify the contributions of individual presynaptic neurons - including inhibitory and excitatory synaptic inputs - to postsynaptic potentials, which enables us to study dendritic integration...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28424417/mesenchymal-stem-cells-differentially-affect-the-invasion-of-distinct-glioblastoma-cell-lines
#8
Barbara Breznik, Helena Motaln, Miloš Vittori, Ana Rotter, Tamara Lah Turnšek
Glioblastoma multiforme are an aggressive form of brain tumors that are characterized by distinct invasion of single glioblastoma cells, which infiltrate the brain parenchyma. This appears to be stimulated by the communication between cancer and stromal cells. Mesenchymal stem cells (MSCs) are part of the glioblastoma microenvironment, and their 'cross-talk' with glioblastoma cells is still poorly understood. Here, we examined the effects of bone marrow-derived MSCs on two different established glioblastoma cell lines U87 and U373...
April 11, 2017: Oncotarget
https://www.readbyqxmd.com/read/28421519/calcite-precipitates-in-slovenian-bottled-waters
#9
Tamara Ferjan Stanič, Miloš Miler, Mihael Brenčič, Mateja Gosar
Storage of bottled waters in varying ambient conditions affects its characteristics. Different storage conditions cause changes in the initial chemical composition of bottled water which lead to the occurrence of precipitates with various morphologies. In order to assess the relationship between water composition, storage conditions and precipitate morphology, a study of four brands of Slovenian bottled water stored in PET bottles was carried out. Chemical analyses of the main ions and measurements of the physical properties of water samples were performed before and after storage of water samples at different ambient conditions...
April 18, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28421385/in-vitro-and-in-vivo-anti-tumor-effects-of-selected-platinum-iv-and-dinuclear-platinum-ii-complexes-against-lung-cancer-cells
#10
Milos Arsenijevic, Marija Milovanovic, Snezana Jovanovic, Natalija Arsenijevic, Bojana Simovic Markovic, Marina Gazdic, Vladislav Volarevic
In the present study, cytotoxic effects of cisplatin, the most usually used chemotherapeutic agent, were compared with new designed platinum(IV) ([PtCl4(en)] (en = ethylenediamine) and [PtCl4(dach)]) (dach = (±)-trans-1,2-diaminocyclohexane) and platinum(II) complexes ([{trans-Pt(NH3)2Cl}2(μ-pyrazine)](ClO4)2 (Pt1), [{trans-Pt(NH3)2Cl}2(μ-4,4'-bipyridyl)](ClO4)2DMF(Pt2),[{trans-Pt(NH3)2Cl}2(μ-1,2-bis(4pyridyl)ethane)](ClO4)2 (Pt3)), in vitro and in vivo against human and murine lung cancer cells, to determine anti-tumor potential of newly synthesized platinum-based drugs in the therapy of lung cancer...
April 18, 2017: Journal of Biological Inorganic Chemistry: JBIC
https://www.readbyqxmd.com/read/28420971/dynamic-increase-in-corticomuscular-coherence-during-bilateral-cyclical-ankle-movements
#11
Takashi Yoshida, Kei Masani, Karl Zabjek, Robert Chen, Milos R Popovic
In humans, the midline primary motor cortex is active during walking. However, the exact role of such cortical participation is unknown. To delineate the role of the primary motor cortex in walking, we examined whether the primary motor cortex would activate leg muscles during movements that retained specific requirements of walking (i.e., locomotive actions). We recorded electroencephalographic and electromyographic signals from 15 healthy, young men while they sat and performed bilateral, cyclical ankle movements...
2017: Frontiers in Human Neuroscience
https://www.readbyqxmd.com/read/28417538/synthesis-of-long-oxahelicenes-by-polycyclization-in-a-flow-reactor
#12
Jindřich Nejedlý, Michal Šámal, Jiří Rybáček, Miroslava Tobrmanová, Florence Szydlo, Christophe Coudret, Maria Neumeier, Jaroslav Vacek, Jana Vacek Chocholoušová, Miloš Buděšínský, David Šaman, Lucie Bednárová, Ladislav Sieger, Irena G Stará, Ivo Starý
A series of oxahelicenes composed of ortho/meta-annulated benzene/pyridine and 2H-pyran rings were synthesized on the basis of the cobalt(I)-mediated (or rhodium(I)- or nickel(0)-mediated) double, triple, or quadruple [2+2+2] cycloisomerization of branched aromatic hexa-, nona-, or dodecaynes, thus allowing the construction of 6, 9, or 12 rings in a single operation. The use of a flow reactor was found to be beneficial for the multicyclization reactions. The stereogenic centers present in some of the oligoynes steered the helical folding in such a way that the final oxa[9]-, [13]-, [17]- and [19]helicenes were obtained in both enantiomerically and diastereomerically pure form...
April 18, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28416270/plasma-levels-and-leucocyte-rna-expression-of-adipokines-in-young-patients-with-coronary-artery-disease-in-metabolic-syndrome-and-healthy-controls
#13
Aleš Smékal, Jan Václavík, David Stejskal, Klára Benešová, Jiří Jarkovský, Gabriela Svobodová, Radmila Richterová, Marek Švesták, Miloš Táborský
OBJECTIVES: Little is known about the role of adipokines in the pathogenesis of coronary artery disease in young patients. The aims of this study were to compare serum levels of adipokines and expression of adipokines in peripheral blood leukocytes in patients with premature coronary artery disease (CAD), metabolic syndrome and healthy individuals. DESIGN AND METHODS: Sixty-five patients with premature CAD (men 18-45years old and women 18-55years old) formed the study group...
April 14, 2017: Cytokine
https://www.readbyqxmd.com/read/28415984/comparison-of-three-different-methods-for-risk-adjustment-in-neonatal-medicine
#14
Mark Adams, Julia Braun, Hans Ulrich Bucher, Milo Alan Puhan, Dirk Bassler, Viktor Von Wyl
BACKGROUND: Quality improvement in health care requires identification of areas in need of improvement by comparing processes and patient outcomes within and between health care providers. It is critical to adjust for different case-mix and outcome risks of patient populations but it is currently unclear which approach has higher validity and how limitations need to be dealt with. Our aim was to compare 3 approaches towards risk adjustment for 7 different major quality indicators in neonatal intensive care (21 models)...
April 17, 2017: BMC Pediatrics
https://www.readbyqxmd.com/read/28415016/an-lc-ms-ms-method-for-the-determination-of-antibiotic-residues-in-distillers-grains
#15
Kaleb J Duelge, Upul Nishshanka, Hemakanthi G De Alwis
Antibiotics are used in ethanol production to discourage the growth of bacteria that would result in lower ethanol content and a lower quality product. A survey conducted by the FDA (FY 2010 Nationwide Survey of Distillers Grains for Antibiotic Residues, 2009 [1]) revealed that the residues of these antibiotics can remain in the distillers grains (DG) by-product, which is used as an animal feed ingredient. The low levels of antibiotic residues in DG could be a public health concern, as they could lead to antimicrobial resistance...
April 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28405233/novel-%C3%AE-cyclodextrin-eosin-conjugates
#16
Gábor Benkovics, Damien Afonso, András Darcsi, Szabolcs Béni, Sabrina Conoci, Éva Fenyvesi, Lajos Szente, Milo Malanga, Salvatore Sortino
Eosin B (EoB) and eosin Y (EoY), two xanthene dye derivatives with photosensitizing ability were prepared in high purity through an improved synthetic route. The dyes were grafted to a 6-monoamino-β-cyclodextrin scaffold under mild reaction conditions through a stable amide linkage using the coupling agent 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride. The molecular conjugates, well soluble in aqueous medium, were extensively characterized by 1D and 2D NMR spectroscopy and mass spectrometry...
2017: Beilstein Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28402650/comprehensive-analytical-approach-toward-glycomic-characterization-and-profiling-in-urinary-exosomes
#17
Guozhang Zou, John D Benktander, Solomon T Gizaw, Stefan Gaunitz, Milos V Novotny
Exosomes are extracellular nano-sized vesicles with lipid bilayers encapsulating nucleic acids and proteins, both with and without glycosylation. While exosomal nucleic acids and proteins have previously been explored to identify cancer biomarkers with some promising results, little information has been available concerning their glycoconjugate content. Herein, we developed analytical methods to investigate glycosylation of urinary exosomes for future diagnostic and prognostic measurements pertaining to the malignancies of genitourinary tract...
April 12, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28402644/investigation-of-the-halogenate-hydrogen-peroxide-reactions-using-the-electron-paramagnetic-resonance-spin-trapping-technique
#18
Maja C Pagnacco, Miloš D Mojović, Ana D Popović-Bijelić, Attila K Horváth
The differences in the mechanism of the halogenate reactions with the same oxidizing/reducing agent, such as H2O2 contribute to the better understanding of versatile halogen chemistry. The reaction between iodate, bromate, and chlorate with hydrogen peroxide in acidic medium at 60 °C is investigated by using the electron paramagnetic resonance (EPR) spin trapping technique. Essential differences in the chemistry of iodate, bromate, and chlorate in their reactions with hydrogen peroxide have been evidenced by finding different radicals as governing intermediates...
April 20, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28399493/increasing-strength-of-a-biomedical-ti-nb-ta-zr-alloy-by-alloying-with-fe-si-and-o
#19
Josef Stráský, Petr Harcuba, Kristína Václavová, Klaudia Horváth, Michal Landa, Ondřej Srba, Miloš Janeček
Low-modulus biomedical beta titanium alloys often suffer from low strength which limits their use as load-bearing orthopaedic implants. In this study, twelve different Ti-Nb-Zr-Ta based alloys alloyed with Fe, Si and O additions were prepared by arc melting and hot forging. The lowest elastic modulus (65GPa) was achieved in the benchmark TNTZ alloy consisting only of pure β phase with low stability due to the 'proximity' to the β to α'' martensitic transformation. Alloying by Fe and O significantly increased elastic modulus, which correlates with the electrons per atom ratio (e/a)...
April 2, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28398520/injury-leads-to-the-appearance-of-cells-with-characteristics-of-both-microglia-and-astrocytes-in-mouse-and-human-brain
#20
Ulrika Wilhelmsson, Daniel Andersson, Yolanda de Pablo, Roy Pekny, Anders Ståhlberg, Jan Mulder, Nicholas Mitsios, Tibor Hortobágyi, Milos Pekny, Marcela Pekna
Microglia and astrocytes have been considered until now as cells with very distinct identities. Here, we assessed the heterogeneity within microglia/monocyte cell population in mouse hippocampus and determined their response to injury, by using single-cell gene expression profiling of cells isolated from uninjured and deafferented hippocampus. We found that in individual cells, microglial markers Cx3cr1, Aif1, Itgam, and Cd68 were co-expressed. Interestingly, injury led to the co-expression of the astrocyte marker Gfap in a subpopulation of Cx3cr1-expressing cells from both the injured and contralesional hippocampus...
April 7, 2017: Cerebral Cortex
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