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Islet cell transplantation

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https://www.readbyqxmd.com/read/29661473/silk-fibroin-preserves-beta-cell-function-under-inflammatory-stress-while-stimulating-islet-cell-surface-glut2-expression
#1
N Ashari, H W Pang, T Simon, Y Xiong, J M Coburn, J S Bromberg, D L Kaplan, J McLenithan, M J Fontaine
Silk fibroin is a novel biomaterial for enhancing transplanted islet cell function and survival. This study investigated whether silk fibroin may have unique properties that improve islet function in the face of inflammatory-mediated stress during transplantation. Murine islet function was tested in vitro with either silk fibroin or alginate and challenged with inflammatory cytokines. The glucose-stimulated insulin secretion index for all conditions decreased with inflammatory cytokines, but was better preserved for islets exposed to silk compared to those exposed to alginate or medium...
April 9, 2018: Cellular Immunology
https://www.readbyqxmd.com/read/29622345/early-islets-and-mesenchyme-from-an-injured-adult-pancreas-improve-syngeneic-engraftments-and-islet-graft-function-in-diabetic-rats
#2
Venant Tchokonte-Nana, Juziel Kampando Manda
A decrease in mass of isografts and a decline in islet function are major challenges in islet transplantations. Despite this, transplantation of 84 h harvested pancreatic duct ligation (PDL) tissues have been shown to have the same functional ability to foetal pancreata, but there was only 40% success in reverting hyperglycaemia. We tested the potential of early islets with mesenchymal stromal cells (MSCs) to promote isogeneic grafts survival and to restore normoglycemia in diabetic rats, in comparison with late islets...
April 2, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29620617/pancreas-transplantation-from-pediatric-donors-a-single-center-experience
#3
Mario Spaggiari, Caterina Di Bella, Pierpaolo Di Cocco, Maya Campara, Kelly Galen, Federico Gheza, Jose Oberholzer, Enrico Benedetti, Ivo Tzvetanov
BACKGROUND: Pancreas allografts from pediatric donors are considered less suitable due to the increased risk of surgical complications and reduced islet cell mass that may compromise function. METHODS: All pancreatic transplants, procured from donors <18 years old, between January 2007 and March 2017, were included in the analysis. The grafts were subdivided into 3 groups by donor's weight: <30kg, 30-60kg, >60kg. Analysis of patient and graft survival was done between the groups, and subsequently between the pediatric cohort and the adult-donor control group...
April 3, 2018: Transplantation
https://www.readbyqxmd.com/read/29611212/design-of-bioartificial-pancreases-from-the-standpoint-of-oxygen-supply
#4
Hiroo Iwata, Yusuke Arima, Yusuke Tsutsui
A bioartificial pancreas (BAP), in which islets of Langerhans (islets) are enclosed in a semipermeable membrane, has been developed to realize islet transplantation without the use of immunosuppressive drugs. Although recent progress in induced pluripotent stem (iPS) and embryonic stem (ES) cells has attracted attention owing to the potential applications of these cells as insulin-releasing cells, concerns about the safety of implantation of these cells remain. The use of the BAP has the advantage of easy removal if insulin-releasing cells derived from iPS/ES cells undesirably proliferate and form tumors in the BAP...
April 2, 2018: Artificial Organs
https://www.readbyqxmd.com/read/29609688/pdx1-transfection-induces-human-adipose-derived-stem-cells-differentiation-into-islet-like-cells-what-is-the-benefit-for-diabetic-rats
#5
Jian Gao, Yue Yuan, Yuqing Chen
In this study, we observed the differentiation potential of human ADMSCs (hADMSCs) into functional islet-like cells and the therapeutic effect of hADMSCs transplantation in diabetic rats. Firstly, the PDX1 gene was transfected into hADMSCs by an adenovirus. Cell differentiation and insulin secretion were identified and detected by dithizone staining and ELISA, respectively. Twenty male Sprague-Dawley rats were randomly divided into control group (n=4), diabetes group (n=8) and transplantation group (n=8). Rats in the latter two groups were subjected to making diabetic models by 65 mg/kg streptozotocin injection...
April 2, 2018: Die Pharmazie
https://www.readbyqxmd.com/read/29606260/-transplantation-strategy-in-type-1-diabetic-patients
#6
Fanny Buron, Lionel Badet, Emmanuel Morelon
Beta cell replacement by pancreas or Langerhans islets transplantation is the only way to restore glucose homeostasis in type 1 diabetic patients. The counterpart is the need for long-term immunosuppression. These transplantations are therefore mainly indicated for patients candidates for kidney transplantation and for patients with poor quality of life due to unstable diabetes with life-threatening hypoglycemic events. Both beta cell replacement techniques have different benefits and risks and should be adapted to each type 1 diabetic patient...
April 2018: Néphrologie & Thérapeutique
https://www.readbyqxmd.com/read/29601985/mechanical-characterization-and-numerical-simulation-of-a-subcutaneous-implantable-3d-printed-cell-encapsulation-system
#7
Federica Adamo, Marco Farina, Usha R Thekkedath, Alessandro Grattoni, Raffaella Sesana
Cell transplantation in bioengineered scaffolds and encapsulation systems has shown great promise in regenerative medicine. Depending on the site of implantation, type of cells and their expected function, these systems are designed to provide cells with a physiological-like environment while providing mechanical support and promoting long-term viability and function of the graft. A minimally invasive 3D printed system termed neovascularized implantable cell homing and encapsulation (NICHE) was developed in polylactic acid for subcutaneous transplantation of endocrine cells, including pancreatic islets...
March 21, 2018: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/29601863/culturing-functional-pancreatic-islets-on-%C3%AE-5-laminins-and-curative-transplantation-to-diabetic-mice
#8
Kristmundur Sigmundsson, Juha R M Ojala, Miina K Öhman, Anne-May Österholm, Aida Moreno-Moral, Anna Domogatskaya, Li Yen Chong, Yang Sun, Xiaoran Chai, Joseph A M Steele, Benjamin George, Manuel Patarroyo, Ann-Sofie Nilsson, Sergey Rodin, Sujoy Ghosh, Molly M Stevens, Enrico Petretto, Karl Tryggvason
The efficacy of islet transplantation for diabetes treatment suffers from lack of cadaver-derived islets, islet necrosis and long transfer times prior to transplantation. Here, we developed a method for culturing mouse and human islets in vitro on α5-laminins, which are natural components of islet basement membranes. Adhering islets spread to form layers of 1-3 cells in thickness and remained normoxic and functional for at least 7 days in culture. In contrast, spherical islets kept in suspension developed hypoxia and central necrosis within 16 h...
March 27, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/29599125/establishment-of-human-pluripotent-stem-cell-derived-pancreatic-%C3%AE-like-cells-in-the-mouse-pancreas
#9
Haiting Ma, Katherine J Wert, Dmitry Shvartsman, Douglas A Melton, Rudolf Jaenisch
Type 1 diabetes is characterized by autoimmune destruction of β cells located in pancreatic islets. However, tractable in vivo models of human pancreatic β cells have been limited. Here, we generated xenogeneic human pancreatic β-like cells in the mouse pancreas by orthotopic transplantation of stem cell-derived β (SC-β) cells into the pancreas of neonatal mice. The engrafted β-like cells expressed β cell transcription factors and markers associated with functional maturity. Engrafted human cells recruited mouse endothelial cells, suggesting functional integration...
March 29, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29597032/current-advanced-therapy-cell-based-medicinal-products-for-type-1-diabetes-treatment
#10
REVIEW
Alberto Cañibano-Hernández, Laura Sáenz Del Burgo, Albert Espona-Noguera, Jesús Ciriza, Jose Luis Pedraz
In the XXI century diabetes mellitus has become one of the main threats to human health with higher incidence in regions such as Europe and North America. Type 1 diabetes mellitus (T1DM) occurs as a consequence of the immune-mediated destruction of insulin producing β-cells located in the endocrine part of the pancreas, the islets of Langerhans. The administration of exogenous insulin through daily injections is the most prominent treatment for T1DM but its administration is frequently associated to failure in glucose metabolism control, finally leading to hyperglycemia episodes...
March 27, 2018: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29593968/engineering-a-tumor-microenvironment-mimetic-niche-for-tissue-regeneration-with-xenogeneic-cancer-cells
#11
Zhenzhen Wang, Chunming Wang, Ayipaxia Abudukeremu, Xiaying Rui, Shang Liu, Xiaoyi Zhang, Min Zhang, Junfeng Zhang, Lei Dong
The insufficient number of cells suitable for transplantation is a long-standing problem to cell-based therapies aimed at tissue regeneration. Xenogeneic cancer cells (XCC) may be an alternative source of therapeutic cells, but their transplantation risks both immune rejection and unwanted spreading. In this study, a strategy to facilitate XCC transplantation is reported and their spreading in vivo is confined by constructing an engineering matrix that mimics the characteristics of tumor microenvironment. The data show that this matrix, a tumor homogenate-containing hydrogel (THAG), successfully creates an immunosuppressive enclave after transplantation into immunocompetent mice...
March 2018: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://www.readbyqxmd.com/read/29575787/sequential-coating-of-insulin-secreting-beta-cells-within-multilayers-of-polysaccharide-nanogels
#12
Tugba Bal, Dilem Ceren Oran, Yoshihiro Sasaki, Kazunari Akiyoshi, Seda Kizilel
Pancreatic islet transplantation has emerged as a promising treatment for type-1 diabetes (T1D); however, its clinical application is still limited by the life-long use of immunosuppressive drugs, insufficient number of islets to achieve normoglycemia, and large transplantation volume. This paper reports a unique approach for nanothin coating of insulin secreting beta cell aggregates. The coating is based on hydrophobic and covalent interactions between natural acrylate modified cholesterol bearing pullulan (CHPOA) nanogels and MIN6 beta cell aggregates...
March 25, 2018: Macromolecular Bioscience
https://www.readbyqxmd.com/read/29569832/bone-marrow-derived-mesenchymal-stem-cells-protect-islet-grafts-against-endoplasmic-reticulum-stress-induced-apoptosis-during-the-early-stage-after-transplantation
#13
Ying He, Dongmei Zhang, Yi Zeng, Junlei Ma, Jing Wang, Hui Guo, Ji Zhang, Mengqin Wang, Weijie Zhang, Nianqiao Gong
Early loss of grafted islets is the main obstacle to achieve favorable outcomes of islet transplantation. Mesenchymal stem cells are known to have a protective effect; however, its mechanism remains unclear. We hypothesized that bone marrow-derived mesenchymal stem cells (BMSCs) can protect grafted islets against endoplasmic reticulum stress (ERS)-induced apoptosis. In syngeneic streptozocin-induced diabetic BALB/c mice, islet grafts decreased blood glucose levels; however, the effect was not fully functional from the immediate post-transplant phase...
March 23, 2018: Stem Cells
https://www.readbyqxmd.com/read/29568862/overexpression-of-appoptosin-promotes-mitochondrial-damage-in-min6-cells
#14
Tianxi Wang, Wenjing Wei, Hussen Amir Ahmed Mansai, Caoxin Huang, Long Li, Qiuhong Ye, Hongyan Yin, Chen Yang, Xuejun Li, Suhuan Liu, Shuyu Yang
Damage to pancreatic β‑cells is closely associated with diabetes. However, the mechanism underlying injury to pancreatic β‑cells remains unclear, although hypoxia is considered as one of the leading causes. Appoptosin is a mitochondrial protein that promotes neuronal apoptosis. Studies conducted on appoptosin thus far have primarily focused on Alzheimer's disease, and have demonstrated that the expression of appoptosin is significantly increased in ischemic‑reperfused rat brains, which indicates its close association with hypoxia...
March 16, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29568778/integration-of-routine-parameters-of-glycemic-variability-in-a-simple-screening-method-for-partial-remission-in-children-with-type-1-diabetes
#15
Nina Nielens, Olivier Pollé, Annie Robert, Philippe A Lysy
Although different criteria were used to define partial remission in type 1 diabetes, the IDAA1C formula has prevailed as it correlates with stimulated C-peptide levels. Our retrospective study evaluated clinical variables associated with the occurrence of IDAA1C-defined partial remission in a series of 239 pediatric patients. Diabetic ketoacidosis and age at diagnosis, but no other clinical feature, influenced the occurrence of remission. We then evaluated whether parameters of glycemic variability used in clinical routine may reliably define partial remission, as these would alleviate confounding factors related to insulin treatment...
2018: Journal of Diabetes Research
https://www.readbyqxmd.com/read/29565508/islet-transplantation-30-years-after-the-first-transplants
#16
B Antonioli, M Galuzzi
First clinical islet allotransplantation in patients affected by type 1 diabetes mellitus was performed about 30 years ago. Despite the progressive improvement of the success rate, the clinical indication to the islet allotransplantation remains limited to selected patients affected by brittle type 1 diabetes mellitus. The burden of the immunosuppression therapy still represents the main critical issue but other areas might be subject to further improvements, such as the islet production, islet engraftment and long-term function...
March 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29563123/murine-cmv-induces-type-1-ifn-that-impairs-differentiation-of-mdscs-critical-for-transplantation-tolerance
#17
Anil Dangi, Lei Zhang, Xiaomin Zhang, Xunrong Luo
Clinical tolerance without immunosuppression has now been achieved for organ transplantation, and its scope will likely continue to expand. In this context, a previously understudied and now increasingly relevant area is how microbial infections might affect the efficacy of tolerance. A highly prevalent and clinically relevant posttransplant pathogen is cytomegalovirus (CMV). Its impact on transplantation tolerance and graft outcomes is not well defined. Employing a mouse model of CMV (MCMV) infection and allogeneic pancreatic islet transplantation in which donor-specific tolerance was induced by infusing donor splenocytes rendered apoptotic by treatment with ethylenecarbodiimide, we investigated the effect of CMV infection on transplantation tolerance induction...
March 27, 2018: Blood Advances
https://www.readbyqxmd.com/read/29555660/the-potential-and-challenges-of-alternative-sources-of-%C3%AE-cells-for-the-cure-of-type-1-diabetes
#18
REVIEW
Monia Cito, Silvia Pellegrini, Lorenzo Piemonti, Valeria Sordi
The experience in the field of islet transplantation shows that it is possible to replace β cells in a patient with type 1 diabetes (T1D), but this cell therapy is limited by the scarcity of organ donors and by the danger associated to the immunosuppressive drugs. Stem cell therapy is becoming a concrete opportunity to treat various diseases. In particular, for a disease like T1D, caused by the loss of a single specific cell type that does not need to be transplanted back in its originating site to perform its function, a stem cell-based cell replacement therapy seems to be the ideal cure...
March 2018: Endocrine Connections
https://www.readbyqxmd.com/read/29546813/endothelial-and-beta-cell-composite-aggregates-for-improved-function-of-a-bioartificial-pancreas-encapsulation-device
#19
Katarzyna Skrzypek, Yazmin Brito Barrera, Thomas Groth, Dimitrios Stamatialis
INTRODUCTION: Encapsulation of pancreatic islets or beta cells is a promising strategy for treatment of type 1 diabetes by providing an immune isolated environment and allowing for transplantation in a different location than the liver. However, islets used for encapsulation often show lower functionality due to the damaging of islet endothelial cells during the isolation procedure. Factors produced by endothelial cells have great impact on beta cell insulin secretion. Therefore, mutual signaling between endothelial cells and beta cells should be considered for the development of encapsulation systems to achieve high insulin secretion and maintain beta cell viability...
March 2018: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/29534555/improved-labeling-of-pancreatic-islets-using-cationic-magnetoliposomes
#20
Rita Sofia Garcia Ribeiro, Ashwini Ketkar-Atre, Ting Yin, Karim Louchami, Tom Struys, Ivo Lambrichts, Abdullah Sener, Willy Jean Malaisse, Marcel De Cuyper, Uwe Himmelreich
Pancreatic islets (PIs) transplantation is an alternative approach for the treatment of severe forms of type 1 diabetes (T1D). To monitor the success of transplantation, it is desirable to follow the location of engrafted PIs non-invasively. In vivo magnetic resonance imaging (MRI) of transplanted PIs is a feasible cell tracking method; however, this requires labeling with a suitable contrast agent prior to transplantation. We have tested the feasibility of cationic magnetoliposomes (MLs), compared to commercial contrast agents (Endorem and Resovist), by labeling insulinoma cells and freshly isolated rat PIs...
March 12, 2018: Journal of Personalized Medicine
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