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won do heo

Jong S Kim, Eun-Jae Lee, Dae-Il Chang, Jong-Ho Park, Seong Hwan Ahn, Jae-Kwan Cha, Ji Hoe Heo, Sung-Il Sohn, Byung-Chul Lee, Dong-Eog Kim, Hahn Young Kim, Seongheon Kim, Do-Young Kwon, Jei Kim, Woo-Keun Seo, Jun Lee, Sang-Won Park, Seong-Ho Koh, Jin Young Kim, Smi Choi-Kwon
BACKGROUND: Mood and emotional disturbances are common in patients with stroke, and adversely affect the clinical outcome. We aimed to evaluate the efficacy of early administration of escitalopram to reduce moderate or severe depressive symptoms and improve emotional and neurological dysfunction in patients with stroke. METHODS: This was a placebo controlled, double-blind trial done at 17 centres in South Korea. Patients who had had an acute stroke within the past 21 days were randomly assigned in a 1:1 ratio to receive oral escitalopram (10 mg/day) or placebo for 3 months...
January 2017: Lancet Psychiatry
Do-Won Jeong, Sojeong Heo, Jong-Hoon Lee
We assessed the safety of 49 Tetragenococcus halophilus strains isolated from doenjang in Korea. Minimum inhibitory concentration assays showed that all strains can be considered as susceptible to ampicillin, erythromycin, penicillin G, tetracycline, and vancomycin, but resistant to ciprofloxacin based on the Enterococcus breakpoint values provided by the European Committee on Antimicrobial Susceptibility testing in 2015. Ciprofloxacin resistance was sufficiently high to consider the potential for acquisition of transmissible determinants...
April 2017: Food Microbiology
Trang Thi Thu Nguyen, Wei Sun Park, Byung Ouk Park, Cha Yeon Kim, Yohan Oh, Jin Man Kim, Hana Choi, Taeyoon Kyung, Cheol-Hee Kim, Gabsang Lee, Klaus M Hahn, Tobias Meyer, Won Do Heo
Cells migrate by directing Ras-related C3 botulinum toxin substrate 1 (Rac1) and cell division control protein 42 (Cdc42) activities and by polymerizing actin toward the leading edge of the cell. Previous studies have proposed that this polarization process requires a local positive feedback in the leading edge involving Rac small GTPase and actin polymerization with PI3K likely playing a coordinating role. Here, we show that the pleckstrin homology and RhoGEF domain containing G3 (PLEKHG3) is a PI3K-regulated Rho guanine nucleotide exchange factor (RhoGEF) for Rac1 and Cdc42 that selectively binds to newly polymerized actin at the leading edge of migrating fibroblasts...
September 6, 2016: Proceedings of the National Academy of Sciences of the United States of America
Nambin Yim, Seung-Wook Ryu, Kyungsun Choi, Kwang Ryeol Lee, Seunghee Lee, Hojun Choi, Jeongjin Kim, Mohammed R Shaker, Woong Sun, Ji-Ho Park, Daesoo Kim, Won Do Heo, Chulhee Choi
Nanoparticle-mediated delivery of functional macromolecules is a promising method for treating a variety of human diseases. Among nanoparticles, cell-derived exosomes have recently been highlighted as a new therapeutic strategy for the in vivo delivery of nucleotides and chemical drugs. Here we describe a new tool for intracellular delivery of target proteins, named 'exosomes for protein loading via optically reversible protein-protein interactions' (EXPLORs). By integrating a reversible protein-protein interaction module controlled by blue light with the endogenous process of exosome biogenesis, we are able to successfully load cargo proteins into newly generated exosomes...
2016: Nature Communications
Kyung Eun Kim, Youngae Jung, Soonki Min, Miso Nam, Rok Won Heo, Byeong Tak Jeon, Dae Hyun Song, Chin-Ok Yi, Eun Ae Jeong, Hwajin Kim, Jeonghyun Kim, Seon-Yong Jeong, Woori Kwak, Do Hyun Ryu, Tamas L Horvath, Gu Seob Roh, Geum-Sook Hwang
Non-alcoholic fatty liver disease (NAFLD) is one of the most frequent causes of liver disease and its prevalence is a serious and growing clinical problem. Caloric restriction (CR) is commonly recommended for improvement of obesity-related diseases such as NAFLD. However, the effects of CR on hepatic metabolism remain unknown. We investigated the effects of CR on metabolic dysfunction in the liver of obese diabetic db/db mice. We found that CR of db/db mice reverted insulin resistance, hepatic steatosis, body weight and adiposity to those of db/m mice...
2016: Scientific Reports
Jang Won Heo, Bum-Joo Cho, Debra A Goldstein, Yasir J Sepah, Diana V Do, Quan Dong Nguyen
PURPOSE: To investigate the efficacy and safety of fluocinolone acetonide intravitreal implant in patients with Vogt-Koyanagi-Harada disease. METHODS: A post hoc, subgroup analysis on patients with Vogt-Koyanagi-Harada was performed using data sets from two multicenter randomized trials on fluocinolone acetonide implant. Each subject received fluocinolone acetonide implantation in one eye and standard-of-care treatment in the fellow eye and was followed for 3 years...
November 2016: Retina
Hyun Young Woo, Do Young Kim, Jeong Heo, Chang Won Kim, Suk Kim, Ki Tae Yoon, Won Lim, Young Mi Hong, Jong Yun Won, Sang Heon Lee, Kwang Hyub Han, Mong Cho
AIM: The aim of this study was to investigate the effect of yttrium-90 radioembolization on the outcome of Asian patients with early to advanced stage hepatocellular carcinoma (HCC). METHODS: Sixty-two patients were screened and 50 patients (80.6%) were eligible. Response was evaluated using the Response Evaluation Criteria in Solid Tumors (RECIST), and overall survival (OS) was estimated using the Kaplan-Meier method. RESULTS: The Barcelona Clinic Liver Cancer (BCLC) stage was A in 40% of patients, B in 24%, and C in 36%; 66% of patients had hepatitis B virus infections...
June 7, 2016: Hepatology Research: the Official Journal of the Japan Society of Hepatology
Jamel Mankouri, Cheryl Walter, Hazel Stewart, Matthew Bentham, Wei Sun Park, Won Do Heo, Mitsunori Fukuda, Stephen Griffin, Mark Harris
UNLABELLED: The release of infectious hepatitis C virus (HCV) particles from infected cells remains poorly characterized. We previously demonstrated that virus release is dependent on the endosomal sorting complex required for transport (ESCRT). Here, we show a critical role of trans-Golgi network (TGN)-endosome trafficking during the assembly, but principally the secretion, of infectious virus. This was demonstrated by both small interfering RNA (siRNA)-mediated silencing of TGN-associated adaptor proteins and a panel of dominant negative (DN) Rab GTPases involved in TGN-endosome trafficking steps...
August 15, 2016: Journal of Virology
Jin Man Kim, Minji Lee, Nury Kim, Won Do Heo
Cell migration is controlled by various Ca(2+) signals. Local Ca(2+) signals, in particular, have been identified as versatile modulators of cell migration because of their spatiotemporal diversity. However, little is known about how local Ca(2+) signals coordinate between the front and rear regions in directionally migrating cells. Here, we elucidate the spatial role of local Ca(2+) signals in directed cell migration through combinatorial application of an optogenetic toolkit. An optically guided cell migration approach revealed the existence of Ca(2+) sparklets mediated by L-type voltage-dependent Ca(2+) channels in the rear part of migrating cells...
May 24, 2016: Proceedings of the National Academy of Sciences of the United States of America
Sang Kuon Lee, Yoonseok Heo, Joong Min Park, Yong Jin Kim, Seong Min Kim, Do Joong Park, Sang Moon Han, Kyung Won Shim, Yeon Ji Lee, Ja Youn Lee, Jin Won Kwon
PURPOSE: Bariatric surgery is relatively new in Korea, and studies comparing different bariatric procedures in Koreans are lacking. This study aimed to compare the clinical outcomes of laparoscopic adjustable gastric banding (LAGB), Roux-en-Y gastric bypass (RYGB), and sleeve gastrectomy (SG) for treating morbidly obese Korean adults. MATERIALS AND METHODS: In this multicenter retrospective cohort study, we reviewed the medical records of 261 obese patients who underwent different bariatric procedures...
July 2016: Yonsei Medical Journal
Kyung Ah Han, Doyeon Woo, Seungjoon Kim, Gayoung Choii, Sangmin Jeon, Seoung Youn Won, Ho Min Kim, Won Do Heo, Ji Won Um, Jaewon Ko
UNLABELLED: Neurotrophin-3 (NT-3) is a secreted neurotrophic factor that binds neurotrophin receptor tyrosine kinase C (TrkC), which in turn binds to presynaptic protein tyrosine phosphatase σ (PTPσ) to govern excitatory synapse development. However, whether and how NT-3 cooperates with the TrkC-PTPσ synaptic adhesion pathway and TrkC-mediated intracellular signaling pathways in rat cultured neurons has remained unclear. Here, we report that NT-3 enhances TrkC binding affinity for PTPσ...
April 27, 2016: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
Mai Khanh Nguyen, Cha Yeon Kim, Jin Man Kim, Byung Ouk Park, Sangkyu Lee, Hyerim Park, Won Do Heo
Intracellular membrane trafficking, which is involved in diverse cellular processes, is dynamic and difficult to study in a spatiotemporal manner. Here we report an optogenetic strategy, termed light-activated reversible inhibition by assembled trap of intracellular membranes (IM-LARIAT), that uses various Rab GTPases combined with blue-light-induced hetero-interaction between cryptochrome 2 and CIB1. In this system, illumination induces a rapid and reversible intracellular membrane aggregation that disrupts the dynamics and functions of the targeted membrane...
June 2016: Nature Chemical Biology
Zhong-Ji Qian, Won-Kyo Jung, Kyong-Hwa Kang, BoMi Ryu, Se-Kwon Kim, Jae-Young Je, Soo-Jin Heo, Chulhong Oh, Do-Hyung Kang, Won Sun Park, Il-Whan Choi
Microalgae are major primary producers of organic matter in aquatic environments through their photosynthetic activities. Fermented microalga (Pavlova lutheri Butcher) preparation (FMP) is the product of yeast fermentation by Hansenula polymorpha. It was tested for the antioxidant activities including lipid peroxidation inhibitory activity, free-radical-scavenging activity, inhibition of reactive oxygen species (ROS) on mouse macrophages (RAW264.7 cell), and inhibited myeloperoxidase (MPO) activity in human myeloid cells (HL60)...
April 2012: Journal of Phycology
Su-Jin Lee, Youngdeuk Lee, Gun-Hoo Park, Navaneethaiyer Umasuthan, Soo-Jin Heo, Mahanama De Zoysa, Won-Kyo Jung, Dae-Won Lee, Hanjun Kim, Do-Hyung Kang, Chulhong Oh
ʟ-Asparaginase (E.C. is an enzyme involved in asparagine hydrolysis and has the potential to effect leukemic cells and various other cancer cells. We identified the Lasparaginase gene (ʟ-ASPG86) in the genus Mesoflavibacter, which consists of a 1,035 bp open reading frame encoding 344 amino acids. Following phylogenetic analysis, the deduced amino acid sequence of ʟ-ASPG86 (ʟ-ASPG86) was grouped as a type I asparaginase with respective homologs in Escherichia coli and Yersinia pseudotuberculosis...
June 28, 2016: Journal of Microbiology and Biotechnology
Hyerim Park, Sangkyu Lee, Won Do Heo
Optogenetic modules that use genetically encoded elements to control protein function in response to light allow for precise spatiotemporal modulation of signaling pathways. As one of optical approaches, LARIAT (Light-Activated Reversible Inhibition by Assembled Trap) is a unique light-inducible inhibition system that reversibly sequesters target proteins into clusters, generated by multimeric proteins and a blue light-induced heterodimerization module. Here we present a method based on LARIAT for optical inhibition of targets in living mammalian cells...
2016: Methods in Molecular Biology
Nury Kim, Jin Man Kim, Won Do Heo
FGFR1 is a member of the fibroblast growth factor family, which controls diverse cellular functions such as cell proliferation, migration, and differentiation. OptoFGFR1, an optogenetic method to modulate the FGFR signaling pathway with light by utilizing PHR domain of cryptochrome2 and cytoplasmic region of FGFR1, enabled light-guided activation of FGFR to study its effects on downstream signaling pathway and during diverse biological processes such as cell migration. Here, we describe about optogenetic and microscopic methods to spatiotemporally manipulate FGFR signaling in a single cell or group of cells using confocal microscope and LED array...
2016: Methods in Molecular Biology
Se-Hoon Hong, Won Jae Lee, Young Doo Kim, Hyunjoo Kim, Young-Jun Jeon, Bitna Lim, Dong-Hyung Cho, Won Do Heo, Doo-Hyun Yang, Chan-Young Kim, Han-Kwang Yang, Jin Kuk Yang, Yong-Keun Jung
Despite the fact that the epidermal growth factor (EGF) family member ERBB3 (HER3) is deregulated in many cancers, the list of ERBB3-interacting partners remains limited. Here, we report that the Apaf-1-interacting protein (APIP) stimulates heregulin-β1 (HRG-β1)/ERBB3-driven cell proliferation and tumorigenesis. APIP levels are frequently increased in human gastric cancers and gastric cancer-derived cells. Cell proliferation and tumor formation are repressed by APIP downregulation and stimulated by its overexpression...
April 19, 2016: Oncotarget
Hee-Yeon Kim, Joong-jae Lee, Nury Kim, Won Do Heo, Hak-Sung Kim
Probing protein-protein interactions in living cells is crucial for understanding the protein functions and developing drugs. Small-sized protein binders are considered effective and useful for such analysis. Here we describe the development and use of a repebody, which is a protein binder composed of LRR (Leucine-rich repeat) modules, for tracking protein-protein interaction and localization in real-time through live-cell imaging. A repebody with high affinity for a red fluorescent protein was selected through a phage display, fused with a green fluorescent protein, and applied for tracing a red fluorescent protein-fused target protein in mammalian cells...
February 19, 2016: Biochemical and Biophysical Research Communications
Jeong-Hyun Ryou, Yoo-Kyoung Sohn, Da-Eun Hwang, Woo-Yong Park, Nury Kim, Won-Do Heo, Mi-Young Kim, Hak-Sung Kim
The intracellular delivery of proteins with high efficiency in a receptor-specific manner is of great significance in molecular medicine and biotechnology, but remains a challenge. Herein, we present the development of a highly efficient and receptor-specific delivery platform for protein cargos by combining the receptor binding domain of Escherichia coli Shiga-like toxin and the translocation domain of Pseudomonas aeruginosa exotoxin A. We demonstrated the utility and efficiency of the delivery platform by showing a cytosolic delivery of diverse proteins both in vitro and in vivo in a receptor-specific manner...
August 2016: Biotechnology and Bioengineering
Abdulkadhar Mohamed Jalaludeen, Woo Tae Ha, Ran Lee, Jin Hoi Kim, Jeong Tae Do, Chankyu Park, Young Tae Heo, Won Young Lee, Hyuk Song
Biochanin A (BCA) is a natural organic compound of the phytoestrogenic isoflavone class that has antioxidant and metal chelator properties in the presence of transition metal ions, however, its efficacy in animal models is still obscure. Therefore, the objective of this study was to investigate the protective effects of BCA against arsenic-induced hepatic injury and hematotoxicity in rats. The results suggest that arsenic intoxicated rats showed significantly higher levels of plasma hepatic markers than normal control rats...
January 9, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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