Dan Yu, Michelle A Baird, John R Allen, Elizabeth S Howe, Matthew P Klassen, Anna Reade, Kalpana Makhijani, Yuanquan Song, Songmei Liu, Zehra Murthy, Shao-Qing Zhang, Orion D Weiner, Thomas B Kornberg, Yuh-Nung Jan, Michael W Davidson, Xiaokun Shu
Infrared fluorescent proteins (IFPs) provide an additional color to GFP and its homologs in protein labeling. Drawing on structural analysis of the dimer interface, we identified a bacteriophytochrome in the sequence database that is monomeric in truncated form and engineered it into a naturally monomeric IFP (mIFP). We demonstrate that mIFP correctly labels proteins in live cells, Drosophila and zebrafish. It should be useful in molecular, cell and developmental biology.
August 2015: Nature Methods