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Hiroya Iida, Masaki Kaibori, Shogo Tanaka, Shigekazu Takemura, Hiroshi Wada, Fumitoshi Hirokawa, Takuya Nakai, Michihiro Hayashi, Hidetoshi Eguchi, Shoji Kubo
BACKGROUND AND OBJECTIVES: We determined the rates of initial lymph node metastasis following curative resection of mass-forming type-intrahepatic cholangiocarcinoma (ICC) in patients with and without hepatitis virus infection. METHODS: We enrolled 87 patients between January 2000 and December 2013 with ICC without preoperative lymph node metastasis and without lymph node dissection. Patients included 32 who were seropositive for hepatitis B or C virus (virus group) and 55 who had no evidence of hepatitis virus infection (nonvirus group)...
November 28, 2016: World Journal of Surgery
Yoshihiro Inoue, Yoshiro Imai, Kensuke Fujii, Fumitoshi Hirokawa, Michihiro Hayashi, Kazuhisa Uchiyama
PURPOSE: The purpose of this retrospective study was to evaluate the utility of the new intraoperative bile leakage test as a preventive measure of postoperative bile leakage. METHOD: 737 patients were retrospectively analyzed with respect to the management of intra- and post-operative bile leakage. RESULTS: Nine (8.3%) of 109 patients evaluated using conventional white light fluorescent imaging were recognized as having intra-operative bile leakage...
October 8, 2016: American Journal of Surgery
M Higuchi, M Hirokawa, A Suzuki, N Takada, N Yamao, S Kuma, A Miyauchi
OBJECTIVE: Solid variants of papillary thyroid carcinoma (SV-PTC) are rare, and there have been few reports describing the cytological findings of such variants. METHODS: The cytological features of cellular specimens aspirated from 18 histologically confirmed SV-PTC cases were evaluated, retrospectively. RESULTS: Solid and small papillary clusters were observed in 14 (77.8%) and 13 (72.2%) cases, respectively. The incidences of large papillary clusters (11...
November 13, 2016: Cytopathology: Official Journal of the British Society for Clinical Cytology
Alyaksandr Nikitski, Tatiana Rogounovitch, Andrey Bychkov, Meiko Takahashi, Koh-Ichiro Yoshiura, Norisato Mitsutake, Takahisa Kawaguchi, Michiko Matsuse, Valentina M Drozd, Yuri E Demidchik, Eijun Nishihara, Mitsuyoshi Hirokawa, Akira Miyauchi, Alexander V Rubanovich, Fumihiko Matsuda, Shunichi Yamashita, Vladimir A Saenko
BACKGROUND: Several functional SNPs at the FOXE1 locus on chromosome 9q22.33 have been associated with the risk for papillary thyroid carcinoma (PTC). This study set out to elucidate whether their effects are independent, using genotyping results in populations of Asian and European descent. METHODS: Single-nucleotide polymorphisms (SNPs) rs965513 and rs1867277, and a polymorphic region determining the length of FOXE1 polyalanine (poly-Ala) tract were genotyped in 501 patients with PTC and 748 healthy individuals from Japan, and in 660 patients and 820 population controls from Belarus...
November 8, 2016: Thyroid: Official Journal of the American Thyroid Association
Yasuhiro Ito, Mitsuyoshi Hirokawa, Akira Miyauchi, Takuya Higashiyama, Minoru Kihara, Akihiro Miya
AIM: Tall cell variant (TCV) of papillary thyroid carcinoma (PTC) shows a poorer prognosis than conventional PTC. The World Health Organization (WHO) classification defines TCV as the tall cell component (TCC) in ≥50% of PTC lesions. We investigated whether and how the proportion of TCC affects the prognosis of patients with PTC with TCC. PATIENTS AND METHODS: Seventy patients with TCC in ≥30% of their PTC lesions and 210 age- and gender-matched controls with no TCC who underwent locally curative surgery at Kuma Hospital (2006-2014) were enrolled...
November 2, 2016: World Journal of Surgery
Marc Pusztaszeri, Esther Diana Rossi, Manon Auger, Zubair Baloch, Justin Bishop, Massimo Bongiovanni, Ashish Chandra, Beatrix Cochand-Priollet, Guido Fadda, Mitsuyoshi Hirokawa, SoonWon Hong, Kennichi Kakudo, Jeffrey F Krane, Ritu Nayar, Sareh Parangi, Fernando Schmitt, William C Faquin
The Bethesda System for Reporting Thyroid Cytology (TBSRTC) was proposed in 2007 at the National Cancer Institute Thyroid Fine Needle Aspiration State of the Art and Science Conference held in Bethesda, Maryland. The aim was to address the inconsistent and sometimes confusing reporting terminologies used for thyroid FNA throughout the world. The TBSRTC consists of 6 diagnostic categories, each associated with an implied risk of malignancy that translates directly into a clinical management algorithm. Since the publication of the TBSRTC cytology Atlas in January 2010, considerable experience has been gained regarding its application in cytology practice, clinical impact, and limitations...
2016: Acta Cytologica
Yuuta Horioka, Ryuki Kusumoto, Kengo Yamane, Ryosuke Nomura, Takeshi Hirokawa, Kazuaki Ito
A fast and sensitive ion chromatographic method for the simultaneous determinations of six inorganic anions [iodate (IO3(-)), bromate (BrO3(-)), bromide (Br(-)), nitrite (NO2(-)), nitrate (NO3(-)), and iodide (I(-))] in seawater is described using dodecylammonium (DA(+))-coated monolithic octadecylsilyl (ODS) columns, a concentrated aqueous NaCl mobile phase containing dodecylammonium chloride (DAC), and a UV detector. The DA(+)-coated monolithic ODS columns show greater retention for iodate and weaker retention for iodide, compared to dilauryldimethylammonium (DDA(+))-coated monolithic ODS columns...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
Shogo Tanaka, Masaki Kaibori, Masaki Ueno, Hiroshi Wada, Fumitoshi Hirokawa, Takuya Nakai, Hiroya Iida, Hidetoshi Eguchi, Michihiro Hayashi, Shoji Kubo
BACKGROUND: While spontaneously ruptured hepatocellular carcinoma (HCC) has a poor prognosis, the true impact of a rupture on survival after hepatic resection is unclear. METHODS: Fifty-eight patients with ruptured HCC and 1922 with non-ruptured HCC underwent hepatic resection between 2000 and 2013. To correct the difference in the clinicopathological factors between the two groups, propensity score matching (PSM) was used at a 1:1 ratio, resulting in a comparison of 42 patients/group...
December 2016: Journal of Gastrointestinal Surgery: Official Journal of the Society for Surgery of the Alimentary Tract
Eijun Nishihara, Akira Hishinuma, Takahiko Kogai, Nami Takada, Mitsuyoshi Hirokawa, Shuji Fukata, Mitsuru Ito, Tomonori Yabuta, Mitsushige Nishikawa, Hirotoshi Nakamura, Nobuyuki Amino, Akira Miyauchi
BACKGROUND: A germline mutation of KEAP1 gene was reported as a novel genetic abnormality associated with familial multinodular goiter. That report was limited, and the pathogenic features were not well established. PATIENT FINDINGS: We report a 47-year-old Japanese woman who presented with hyperthyroidism and a large multinodular goiter. The family history was notable for a paternal history of goiter. Graves' disease was diagnosed based on positive TRAb, but scintiscan imaging showed that the patient's radioiodine uptake was restricted in the non-nodular areas, indicating largely cold nodules...
2016: Frontiers in Endocrinology
Kimiyo N Yamamoto, Masatsugu Ishii, Yoshihiro Inoue, Fumitoshi Hirokawa, Ben D MacArthur, Akira Nakamura, Hiroshi Haeno, Kazuhisa Uchiyama
Although the capacity of the liver to recover its size after resection has enabled extensive liver resection, post-hepatectomy liver failure remains one of the most lethal complications of liver resection. Therefore, it is clinically important to discover reliable predictive factors after resection. In this study, we established a novel mathematical framework which described post-hepatectomy liver regeneration in each patient by incorporating quantitative clinical data. Using the model fitting to the liver volumes in series of computed tomography of 123 patients, we estimated liver regeneration rates...
October 3, 2016: Scientific Reports
Doudou Wang, Ryo Nitta, Manatsu Morikawa, Hiroaki Yajima, Shigeyuki Inoue, Hideki Shigematsu, Masahide Kikkawa, Nobutaka Hirokawa
The kinesin-8 motor, KIF19A, accumulates at cilia tips and controls cilium length. Defective KIF19A leads to hydrocephalus and female infertility because of abnormally elongated cilia. Uniquely among kinesins, KIF19A possesses the dual functions of motility along ciliary microtubules and depolymerization of microtubules. To elucidate the molecular mechanisms of these functions we solved the crystal structure of its motor domain and determined its cryo-electron microscopy structure complexed with a microtubule...
September 30, 2016: ELife
Togo Shimozawa, Erisa Hirokawa, Fuyu Kobirumaki-Shimozawa, Kotaro Oyama, Seine A Shintani, Takako Terui, Yasuharu Kushida, Seiichi Tsukamoto, Teruyuki Fujii, Shin'ichi Ishiwata, Norio Fukuda
The cardiac pump function is a result of a rise in intracellular Ca(2+) and the ensuing sarcomeric contractions [i.e., excitation-contraction (EC) coupling] in myocytes in various locations of the heart. In order to elucidate the heart's mechanical properties under various settings, cardiac imaging is widely performed in today's clinical as well as experimental cardiology by using echocardiogram, magnetic resonance imaging and computed tomography. However, because these common techniques detect local myocardial movements at a spatial resolution of ∼100 μm, our knowledge on the sub-cellular mechanisms of the physiology and pathophysiology of the heart in vivo is limited...
September 21, 2016: Progress in Biophysics and Molecular Biology
Shinya Uchino, Hideki Ishikawa, Akira Miyauchi, Mitsuyoshi Hirokawa, Shiro Noguchi, Mineko Ushiama, Teruhiko Yoshida, Masahito Michikura, Kokichi Sugano, Toshiyuki Sakai
CONTEXT: The cribriform-morula variant of papillary thyroid carcinoma (CMV-PTC) is a rare variant of papillary thyroid carcinoma (PTC) and is associated with familial adenomatous polyposis (FAP). However, the incidence and the nature of CMV-PTC among FAP patients have not been well characterized. OBJECTIVE: The aim of this study was to determine the incidence and characteristics of thyroid cancer screened by neck ultrasonography for FAP patients. Design, Patients and Intervention: A total of 129 FAP patients were included in this study...
September 13, 2016: Journal of Clinical Endocrinology and Metabolism
Yasutaka Hirokawa, Yudai Dempo, Eiichiro Fukusaki, Taizo Hanai
Cyanobacteria engineered for production of biofuels and biochemicals from carbon dioxide represent a promising area of research in relation to a sustainable economy. Previously, we have succeeded in producing isopropanol from cellular acetyl-CoA by means of Synechococcus elongatus PCC 7942 into which a synthetic metabolic pathway was introduced. The isopropanol production by this synthetic metabolic pathway requires acetate; therefore, the cells grown under photosynthetic conditions have to be transferred to a dark and anaerobic conditions to produce acetate...
September 6, 2016: Journal of Bioscience and Bioengineering
Katsuya Ohbuchi, Yoshikazu Mori, Kazuo Ogawa, Eiji Warabi, Masahiro Yamamoto, Takatsugu Hirokawa
Transient receptor potential vanilloid type 1 (TRPV1) is a non-selective cation channel and a multimodal sensor protein. Since the precise structure of TRPV1 was obtained by electron cryo-microscopy, the binding mode of representative agonists such as capsaicin and resiniferatoxin (RTX) has been extensively characterized; however, detailed information on the binding mode of other vanilloids remains lacking. In this study, mutational analysis of human TRPV1 was performed, and four agonists (capsaicin, RTX, [6]-shogaol and [6]-gingerol) were used to identify amino acid residues involved in ligand binding and/or modulation of proton sensitivity...
2016: PloS One
K Watanabe, C Hirokawa, T Tazawa
Human parechoviruses (HPeVs) mainly infect young children, causing mild gastrointestinal and respiratory diseases; however, HPeV type 3 (HPeV3) causes severe systemic diseases in young infants. To clarify the characteristics of HPeV infections from the aspects of seropositivity and epidemiology, we measured neutralizing antibody titres against HPeVs in individuals of in different age groups and isolated HPeVs from various clinical specimens in Niigata, Japan. The seropositivity to HPeV1, 3, and 6 was higher in older age group...
August 30, 2016: Epidemiology and Infection
Fumihiro Ishikawa, Masato Shirahashi, Hiroshi Hayakawa, Asako Yamaguchi, Takatsugu Hirokawa, Takeshi Tsumuraya, Ikuo Fujii
Taking advantage of antibody molecules to generate tailor-made binding sites, we propose a new class of protein modifications, termed as "site-directed chemical mutation." In this modification, chemically synthesized catalytic components with a variety of steric and electronic properties can be noncovalently and nongenetically incorporated into specific sites in antibody molecules to induce enzymatic activity. Two catalytic antibodies, 25E2 and 27C1, possess antigen-combining sites which bind catalytic components and act as apoproteins in catalytic reactions...
October 21, 2016: ACS Chemical Biology
Yumiko Yamauchi, Shigeharu Ueki, Yasunori Konno, Wataru Ito, Masahide Takeda, Yuka Nakamura, Junko Nishikawa, Yuki Moritoki, Ayumi Omokawa, Tomoo Saga, Makoto Hirokawa
Hepatocyte growth factor (HGF), originally identified as a potent mitogen for mature hepatocytes, is now recognized as a humoral mediator in inflammatory and immune responses. Previous studies indicated that HGF negatively regulated allergic airway inflammation. In view of eosinophils playing a role in the pathogenesis of asthma, especially in airway remodeling as a rich source of pro-fibrogenic mediators, the effects of HGF on the different types of eosinophil secretory functions were examined in this study...
December 2016: Cytokine
Junya Ito, Kiyotaka Nakagawa, Shunji Kato, Takafumi Hirokawa, Shigefumi Kuwahara, Toshiharu Nagai, Teruo Miyazawa
To elucidate the role of enzymatic lipid peroxidation in disease pathogenesis and in food deterioration, we recently achieved stereoselective analysis of phosphatidylcholine hydroperoxide (PCOOH) possessing 13S-hydroperoxy-9Z,11E-octadecadienoic acid (13(S)-9Z,11E-HPODE) using HPLC-MS/MS with a CHIRALPAK OP (+) column. Because enzymatic oxidation progresses concurrently with auto-oxidation, we need to distinguish them further. Here, we attempted such an analysis. First, we used lipoxygenase, linoleic acid, and lysophosphatidylcholine (LPC) to synthesize the enzymatic oxidation product 13(S)-9Z,11E-HPODE PC, and the auto-oxidation products 13(RS)-9Z,11E-HPODE PC and 13(RS)-9E,11E-HPODE PC, which were used as standards to test the ability of various columns to separate the enzymatic oxidation product from auto-oxidation products...
November 2016: Analytical and Bioanalytical Chemistry
Katsuya Ohbuchi, Chika Miyagi, Yasuyuki Suzuki, Yasuharu Mizuhara, Keita Mizuno, Yuji Omiya, Masahiro Yamamoto, Eiji Warabi, Yuka Sudo, Akinobu Yokoyama, Kanako Miyano, Takatsugu Hirokawa, Yasuhito Uezono
Processed Aconiti tuber (PAT) is used to treat pain associated with various disorders. Although it has been demonstrated that the κ opioid receptor (KOR) signaling pathway is a mediator of the analgesic effect of PAT, active components affecting opioid signaling have not yet been identified. In this study, we explored candidate components of PAT by pharmacokinetic analysis and identified ignavine, which is a different structure from aconitine alkaloids. A receptor binding assay of opioid receptors showed that ignavine specifically binds the μ opioid receptor (MOR), not the KOR...
2016: Scientific Reports
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