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https://www.readbyqxmd.com/read/29323530/prevalence-and-molecular-characterization-of-antimicrobial-resistance-in-escherichia-coli-isolated-from-raw-milk-and-raw-milk-cheese-in-egypt
#1
Rabee A Ombarak, Atsushi Hinenoya, Abdel-Rahman M Elbagory, Shinji Yamasaki
The goal of this study was to examine antimicrobial resistance and characterize the implicated genes in 222 isolates of Escherichia coli from 187 samples of raw milk and the two most popular cheeses in Egypt. E. coli isolates were tested for susceptibility to 12 antimicrobials by a disk diffusion method. Among the 222 E. coli isolates, 66 (29.7%) were resistant to one or more antimicrobials, and half of these resistant isolates showed a multidrug resistance phenotype (resistance to at least three different drug classes)...
January 11, 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29315571/salmonella-serotypes-isolated-in-geckos-kept-in-seven-collections-in-southern-italy
#2
T P Russo, L Varriale, L Borrelli, A Pace, M Latronico, L F Menna, A Fioretti, L Dipineto
OBJECTIVES: Reptiles are considered an important reservoir of Salmonella species. This study evaluated the prevalence of Salmonella species in different species of gecko kept as pets in Italy. MATERIALS AND METHODS: Faecal swab samples were collected from 70 clinically healthy geckos and examined for Salmonella species by culture that were then serotyped. RESULTS: Salmonella species were isolated from 24 of 70 (34·3%) samples. Eighteen isolates expressed resistance to ceftazidime and four isolates to ampicillin...
January 9, 2018: Journal of Small Animal Practice
https://www.readbyqxmd.com/read/29315029/correlations-among-resistances-to-different-antimicrobial-compounds-in-salmonella-strains-from-hen-eggshells
#3
Maria Luisa Fernández Márquez, María José Grande Burgos, Rubén Pérez Pulido, Antonio Gálvez, Rosario Lucas López
Persistence of antibiotic-resistant Salmonella in the food chain may depend on strain tolerance to other antimicrobials and also on biofilm formation capacity. Yet, there is limited information on sensitivity of antibiotic-resistant Salmonella to other antimicrobials, such as phenolic compounds, chemical preservatives, or antimicrobial peptides. This study aimed at correlating antimicrobial resistance and biofilm formation capacity in antibiotic-resistant, biocide-tolerant Salmonella strains from hen eggshells...
February 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29311196/non-touch-aseptic-technique-maintains-sterility-of-antibiotic-admixed-peritoneal-dialysis-fluid
#4
Louis L Huang, Ellen Ramas, Priti Prasad, Jenny Catania, Pauline Meade, Eamonn Butler, Lawrence P McMahon
There is a paucity of data on the sterility of peritoneal dialysis fluid (PDF) after drug admixture. International Society for Peritoneal Dialysis (ISPD) guidelines suggest using sterile technique when admixing antibiotics; however, the degree of sterility remains unclear. This issue is most pertinent when preparing take-home PDF for outpatient treatment of peritonitis. This study compares the sterility of PDF admixed with antibiotics using a non-touch aseptic technique (NTAT) versus sterile technique.Groups of 8 PDF mixtures (1...
January 2018: Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis
https://www.readbyqxmd.com/read/29302147/current-trends-of-drug-resistance-patterns-of-acinetobacter-baumannii-infection-in-blood-transfusion-dependent-thalassemia-patients
#5
Suhail Ahmed Almani, Ali Naseer, Sanjay Kumar Maheshwari, Pir Maroof, Raza Naseer, Haji Khan Khoharo
Objective: The present study aimed to evaluate the current trends of drug resistance patterns of Acinetobacter baumannii infection in blood transfusion-dependent thalassemia patients. Study Design: This study was a cross sectional study, conducted at the Liaquat University of Medical and Health Sciences, Jamshoro/Hyderabad, Sindh, Pakistan from October 2014 to January 2016. Subjects and Methods: Of 921 blood samples, A. baumannii strains were isolated from 100 blood samples...
October 2017: Journal of Global Infectious Diseases
https://www.readbyqxmd.com/read/29291486/pharmacodynamic-interactions-of-amikacin-with-selected-%C3%AE-lactams-and-fluoroquinolones-against-canine-escherichia-coli-isolates
#6
Georgios A Delis, Victoria I Siarkou, Elpida I Vingopoulou, Maria Koutsoviti-Papadopoulou, Georgios C Batzias
Knowledge of in vitro antimicrobial interactions can serve as a guide for clinical application of combination antimicrobial regimens. The aim of the present study was to determine the pharmacodynamic interactions of amikacin with either amoxicillin/clavulanic acid, ceftazidime, enrofloxacin or marbofloxacin against clinical canine Escherichia coli isolates. Bactericidal activity of individual antimicrobials was assessed by use of static kill curves. Interactions between amikacin and each of the β-lactams or fluoroquinolones were subsequently analyzed by employing the fractional maximal effect method...
December 18, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29289744/synthesis-spectroscopic-biological-activity-and-thermal-characterization-of-ceftazidime-with-transition-metals
#7
Mamdouh S Masoud, Alaa E Ali, Gehan S Elasala, Sherif A Kolkaila
Synthesis, physicochemical characterization and thermal analysis of ceftazidime complexes with transition metals (Cr(III), Mn(II), Fe(III), Co(II), Ni(II), Cu(II), Zn(II), Cd(II) and Hg(II)) were discussed. It's obtained that ceftazidime act as bidentate ligand. From magnetic measurement and spectral data, octahedral structures were proposed for all complexes except for cobalt, nickel and mercury had tetrahedral structural. Hyper chemistry program confirmed binding sites of ceftazidime. Ceftazidime complexes show higher activity than ceftazidime for some strains...
December 24, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29282402/acute-endophthalmitis-caused-by-leuconostoc-spp-following-intravitreal-bevacizumab-injection
#8
Supreet Singh, Chirag V Patel, Kamal Kishore
We present a case of acute endophthalmitis caused by Leuconostoc spp. following intravitreal bevacizumab injection. An 86-year-old immunocompetent female developed acute endophthalmitis after intravitreal injection of bevacizumab for neovascular age-related macular degeneration. The patient presented with pain, visual acuity of hand motions, hypopyon, and dense vitritis 96 h after treatment. She was treated with vitreous and anterior chamber tap followed by intravitreal injections of 1 mg vancomycin, 2.25 mg ceftazidime, and 400 μg dexamethasone...
September 2017: Case Reports in Ophthalmology
https://www.readbyqxmd.com/read/29274818/population-pharmacokinetics-and-dosing-simulations-of-ceftazidime-in-chinese-neonates
#9
Honghong Wang, Xingang Li, Shusen Sun, Guifu Mao, Ping Xiao, Chan Fu, Zhuoxin Liang, Min Zheng, Yuling Huang, Haihong Tang, Renhao Ou, Ni Yang, Xi Ling, Zhigang Zhao
An accurate dosage determination is required in neonates when antibiotics are used. The adult data cannot be simply extrapolated to the pediatric population due to significant individual differences. We aimed to identify factors impacting ceftazidime exposure in neonates and to provide drug dosing guidance to clinicians. 43 neonates aged less than 60 days with proven or suspected infections were enrolled in this study. After intravenous administration, blood samples were collected, and plasma ceftazidime concentration was determined using a high-performance liquid chromatography method...
December 21, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29274456/molecular-characterization-of-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae-strains-isolated-from-a-tertiary-care-hospital
#10
Jayanti Jena, Nagen Kumar Debata, Rajesh Kumar Sahoo, Mahendra Gaur, Enketeswara Subudhi
Infectious diseases caused by ESBL producing Enterobacteriaceae are an emerging problem worldwide which increases the empirical treatment failure, hospital cost, rate of morbidity and mortality. This also leads to the Hospital infection outbreak. Present study was undertaken to determine the frequency of blaTEM, blaCTX-M and blaSHV genes among Enterobacteriaceae. A total of 751 non-repeated clinical isolates of Enterobacteriaceae family were included in this study. Antibiotic susceptibility test was done and minimal inhibitory concentration (MIC) against four antibiotics was carried out...
December 20, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29273244/bacterial-keratitis-treated-by-strengthened-antibiotic-eye-drops-an-18-months-review-of-clinical-cases-and-antibiotic-susceptibilities
#11
J Saillard, L Spiesser-Robelet, P Gohier, T Briot
PURPOSE: To describe, in patients treated for infectious keratitis, the microorganisms identified and their antibiotic susceptibility over a period of 18 months. METHOD: Retrospective, descriptive, non-comparative study. Medical and biological data were extracted from the patients' file treated with strengthened antibiotic eye drops at Angers University Hospital between January 2015 and June 2016. The main elements noted were the bacteria involved and their susceptibility to antibiotics...
December 19, 2017: Annales Pharmaceutiques Françaises
https://www.readbyqxmd.com/read/29272983/sub-inhibitory-concentration-of-essential-oils-induces-antibiotic-resistance-in-staphylococcus-aureus
#12
Barbara Turchi, Simone Mancini, Luisa Pistelli, Basma Najar, Filippo Fratini
Fourteen Staphylococcus aureus wild strains were stressed with sub-inhibitory concentration of five essential oils: Leptospermum scoparium (manuka), Origanum majorana (marjoram), Origanum vulgare (oregano), Satureja montana (winter savoury) and Thymus vulgaris (thyme). Antibiotics susceptibility profiles of the strains were determined by agar disk diffusion method before and after EOs treatment. The following antibiotics were employed: amoxicillin-clavulanic acid, amikacin, ampicillin, amoxicillin, aztreonam, ceftazidime, cephalothin, ciprofloxacin, colistin, cefotaxime, doxycycline, enrofloxacin, erythromycin, gentamicin, cephalexin, neomycin, piperacillin, rifampin, streptomycin, trimethoprim-sulphamethoxazole, tetracycline and tobramycin...
December 22, 2017: Natural Product Research
https://www.readbyqxmd.com/read/29249044/endogenous-fusarium-endophthalmitis-during-treatment-for-acute-myeloid-leukemia-successfully-treated-with-25-gauge-vitrectomy-and-antifungal-medications
#13
Masaaki Yoshida, Naoki Kiyota, Kazuichi Maruyama, Hiroshi Kunikata, Masahiro Toyokawa, Shigehiro Hagiwara, Koichi Makimura, Naomi Sato, Shinji Taniuchi, Toru Nakazawa
Endogenous fungal endophthalmitis (EFE) caused by disseminated fusariosis is a rare condition that generally has a poor outcome, even with intensive therapy. Here, we describe a case in which this type of EFE was diagnosed with vitreous sampling and was successfully treated with 25-gauge vitrectomy and antifungals, including liposomal amphotericin B and voriconazole. A 16-year-old male patient undergoing treatment for acute myeloid leukemia complained of eye pain and blurred vision in his right eye. Treatment was initiated for a vitreous opacity, possibly associated with herpetic retinitis, but the patient worsened and he was referred to us...
December 16, 2017: Mycopathologia
https://www.readbyqxmd.com/read/29247576/effect-of-non-%C3%AE-lactams-on-stable-variants-of-inhibitor-resistant-tem%C3%A2-%C3%AE-lactamase-in-uropathogenic%C3%A2-e-coli%C3%A2-implication-for-alternative-therapy
#14
Sandip Kumar Mukherjee, Rahul Shubhra Mandal, Santasabuj Das, Mandira Mukherjee
AIMS: β-lactamase inhibitor resistance(BLIR) among the uropathogenic E.coli (UPEC) minimizes treatment options. This study aims to identify inhibitor-resistant TEM (IRT) β-lactamase that impart BLIR phenotype and explore non-β-lactams as alterative therapeutics. METHODS AND RESULTS: 30 BLIR UPEC isolates was detected by Kirby-Bauer disk diffusion technique using β-lactam-β-lactamase inhibitor combination. Conjugal transfer of BLIR was successful from 17 isolates...
December 16, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29244831/antimicrobial-susceptibility-and-molecular-epidemiology-of-clinical-enterobacter-cloacae-bloodstream-isolates-in-shanghai-china
#15
Su Wang, Shu-Zhen Xiao, Fei-Fei Gu, Jin Tang, Xiao-Kui Guo, Yu-Xing Ni, Jie-Ming Qu, Li-Zhong Han
BACKGROUND: Enterobacter cloacae is a major nosocomial pathogen causing bloodstream infections. We retrospectively conducted a study to assess antimicrobial susceptibility and phylogenetic relationships of E. cloacae bloodstream isolates in two tertiary university-affiliated hospitals in Shanghai, in order to facilitate managements of E. cloacae bloodstream infections and highlight some unknowns for future prevention. METHODS: Fifty-three non-duplicate E. cloacae bloodstream isolates were consecutively collected from 2013 to 2016...
2017: PloS One
https://www.readbyqxmd.com/read/29237206/-piperacillin-tazobactam-shortage-central-restriction-and-alternative-recommendations-as-effective-antibiotic-stewardship-intervention-at-a-maximal-care-hospital
#16
Johanna Kessel, Barbara Dolff, Thomas Wichelhaus, Nils Keiner, Michael Hogardt, Claudia Reinheimer, Imke Wieters, Sebastian Harder, Volkhard A J Kempf, Christoph Stephan
Background Drug supply bottleneck is a worldwide challenge, e. g. the antibiotics Piperacillin/Tazobactam shortage in 2016/2017. The efficacy of an appropriate replacement management was evaluated at the University Hospital Frankfurt (UHF). Methods The Antibiotic-Stewardship (ABS)-Team at UHF decreed a restriction of PIP/TAZ and provided alternative antibiotic therapy recommendations during the shortage period. Consequences of this intervention on antibiotic consumption and overall costs were investigated...
December 13, 2017: Deutsche Medizinische Wochenschrift
https://www.readbyqxmd.com/read/29232103/game-changers-new-%C3%AE-lactamase-inhibitor-combinations-targeting-antibiotic-resistance-in-gram-negative-bacteria
#17
Karen Bush
Recent regulatory approvals for the β-lactam inhibitor combinations of ceftazidime-avibactam and meropenem-vaborbactam have provided two novel therapeutic options for the treatment of multidrug-resistant infections caused by Gram-negative bacteria. Most importantly, these combination agents have satisfied an important medical need related to antibiotic-resistant Klebsiella pneumoniae that produce serine carbapenemases, especially the Klebsiella pneumoniae carbapenemase (KPC) enzymes. Both combinations contain non-β-lactam β-lactamase inhibitors of novel chemical classes not previously developed as antibacterial agents, the diazabicyclooctanes and cyclic boronic acid derivatives...
December 12, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/29228202/activity-of-ceftazidime-avibactam-against-problem-enterobacteriaceae-and-pseudomonas-aeruginosa-in-the-uk-2015-16
#18
David M Livermore, Danièle Meunier, Katie L Hopkins, Michel Doumith, Robert Hill, Rachel Pike, Peter Staves, Neil Woodford
Background: Ceftazidime/avibactam combines an established oxyimino-cephalosporin with the first diazabicyclooctane β-lactamase inhibitor to enter clinical use. We reviewed its activity against Gram-negative isolates, predominantly from the UK, referred for resistance investigation in the first year of routine testing, beginning in July 2015. Methods: Isolates were as received from referring laboratories; there is a bias to submit those with suspected carbapenem resistance...
December 8, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29228127/antibiotic-resistance-and-the-risk-of-recurrent-bacteremia
#19
Sjoukje H S Woudt, Sabine C de Greeff, Annelot F Schoffelen, Anne L M Vlek, Marc J M Bonten
Background: Direct health effects of antibiotic resistance are difficult to assess. We quantified the risk of recurrent bacteremia associated with antibiotic resistance. Methods: We extracted antimicrobial susceptibility testing data on blood isolates of Enterobacteriaceae, Pseudomonas spp., Enterococcus spp. and Staphylococcus aureus from the Dutch surveillance system for antimicrobial resistance between 2008 and 2017. First and first recurrent (4-30days) bacteremia episodes were categorized as susceptible, single non-susceptible or co-non-susceptible (I/R), to 3rd generation cephalosporins -/+ carbapenems (Enterobacteriaceae), ceftazidime -/+ carbapenems (Pseudomonas spp...
December 7, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29216906/detection-of-metallo-%C3%AE-lactamases-encoding-genes-among-clinical-isolates-of-pseudomonas-aeruginosa-in-a-tertiary-care-hospital-kathmandu-nepal
#20
Mahesh Acharya, Prabhu R Joshi, Kamal Thapa, Rajan Aryal, Trishna Kakshapati, Supriya Sharma
OBJECTIVES: This study was carried out to determine the prevalence of metallo-β-lactamases (MBLs) producing Pseudomonas aeruginosa in imipenem-nonsusceptible isolates and to detect MBL-encoding genes among MBLs-positive isolates. RESULTS: Metallo-β-lactamases production was detected in 68.6% isolates of P. aeruginosa with reduced susceptibility to imipenem. The bla VIM-2 gene was detected in 75% isolates and bla IMP-1 was detected in 25% isolates. All MBLs-positive isolates were multidrug resistant with a high level of resistance to imipenem (MIC 16 to ≥ 32 µg/ml), meropenem (MIC 16 to ≥ 32 µg/ml), and ceftazidime (MIC 64 to ≥ 512 µg/ml)...
December 8, 2017: BMC Research Notes
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