keyword
MENU ▼
Read by QxMD icon Read
search

Rapid blood culture

keyword
https://www.readbyqxmd.com/read/28446575/evaluation-of-strepb-select-chromagar-and-the-fast-track-diagnostics-group-b-streptococcus-gbs-real-time-polymerase-chain-reaction-assay-compared-to-routine-culture-for-detection-of-gbs-during-antepartum-screening
#1
Deirdre L Church, Heather Baxter, Tracie Lloyd, Oscar Larios, Daniel B Gregson
Life-threatening infection in neonates due to Group B Streptococcus (GBS) is preventable by screening near-term pregnant women and treatment at delivery. 295 vaginal-rectal swabs were collected from women attending antepartum clinics in Calgary. GBS colonization was detected by the standard culture method (Strep B Carrot broth subbed to blood agar with a neomycin disk), and compared to recovery using Strep Group B Broth (Dalynn Biologicals) subbed to StrepB Select chromagar (CA) (Bio-Rad Laboratories), and the Fast Track Diagnostics GBS real-time PCR (qPCR) assay (Phoenix Airmid Biomedical Corp...
April 26, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28443247/evaluation-of-a-new-ifn-%C3%AE-release-assay-for-rapid-diagnosis-of-active-tuberculosis-in-a-high-incidence-setting
#2
Gen Li, Feng Li, Hui-Min Zhao, Han-Li Wen, Hai-Cong Li, Chun-Ling Li, Ping Ji, Peng Xu, Kang Wu, Zhi-Dong Hu, Shui-Hua Lu, Douglas B Lowrie, Jian-Xin Lv, Xiao-Yong Fan
Blood-based interferon-gamma (IFN-γ) release assays (IGRAs) have been proven to be useful in the diagnosis of Mycobacterium tuberculosis (Mtb) infection. However, IGRAs have not been recommended for clinical practice in most low-income settings due to cost-intensive limitations and shortage of clinical data available. The established T-SPOT. TB assay containing Mtb-specific antigens ESAT-6 and CFP10 are widely used for immunodiagonsis of Mtb infection, but the high cost is one of the restricting factors against its clinical application in the developing countries...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28442767/direct-rapid-antimicrobial-susceptibility-test-from-positive-blood-cultures-based-on-microscopic-imaging-analysis
#3
Jungil Choi, Hyun Yong Jeong, Gi Yoon Lee, Sangkwon Han, Shinhun Han, Bonghwan Jin, Taegeun Lim, Shin Kim, Dong Young Kim, Hee Chan Kim, Eui-Chong Kim, Sang Hoon Song, Taek Soo Kim, Sunghoon Kwon
For the timely treatment of patients with infections in bloodstream and cerebrospinal fluid, a rapid antimicrobial susceptibility test (AST) is urgently needed. Here, we describe a direct and rapid antimicrobial susceptibility testing (dRAST) system, which can determine the antimicrobial susceptibility of bacteria from a positive blood culture bottle (PBCB) in six hours. The positive blood culture sample is directly mixed with agarose and inoculated into a micropatterned plastic microchip with lyophilized antibiotic agents...
April 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28438610/hyperglycemia-increases-interstitial-cells-of-cajal-via-mapk1-and-mapk3-signaling-to-etv1-and-kit-leading-to-rapid-gastric-emptying
#4
Yujiro Hayashi, Yoshitaka Toyomasu, Siva Arumugam Saravanaperumal, Michael R Bardsley, John A Smestad, Andrea Lorincz, Seth T Eisenman, Gianluca Cipriani, Molly H Nelson Holte, Fatimah J Al Khazal, Sabriya A Syed, Gabriella B Gajdos, Kyoung Moo Choi, Gary J Stoltz, Katie E Miller, Michael L Kendrick, Brian P Rubin, Simon J Gibbons, Adil E Bharucha, David R Linden, Louis James Maher, Gianrico Farrugia, Tamas Ordog
BACKGROUND & AIMS: Depletion of interstitial cells of Cajal (ICCs) is common in diabetic gastroparesis. However, in approximately 20% of patients with diabetes, gastric emptying (GE) is accelerated. GE is also faster in obese individuals, and is associated with increased blood levels of glucose in patients with type 2 diabetes. To understand the fate of ICCs in hyperinsulinemic, hyperglycemic states characterized by rapid GE, we studied mice with mutation of the leptin receptor (Lepr(db/db)), which in our colony had accelerated GE...
April 21, 2017: Gastroenterology
https://www.readbyqxmd.com/read/28435765/chronic-nephropathy-from-dietary-hyperoxaluria-sustained-improvement-of-renal-function-after-dietary-intervention
#5
Yijuan Sun, Bruce L Horowitz, Karen S Servilla, Joanna R Fair, Darlene Vigil, Kavitha Ganta, Larry Massie, Antonios H Tzamaloukas
A 56-year-old man with stable chronic kidney disease (CKD) for two years following a single episode of calcium oxalate urolithiasis developed progressive elevation of his serum creatinine concentration. Urinalysis revealed pyuria and white cell casts, a few red blood cells, minimal proteinuria, and no crystals. Urine culture was sterile. Gallium scintigraphy was consistent with interstitial nephritis. Proton pump inhibitor intake was discontinued, and a short course of oral corticosteroids was initiated. Percutaneous kidney biopsy, performed because of the continued deterioration of renal function to a minimum estimated glomerular filtration rate (eGFR) value of 15 mL/min per 1...
March 20, 2017: Curēus
https://www.readbyqxmd.com/read/28433719/isolation-and-characterization-of-monocyte-macrophage-from-peripheral-blood-of-half-smooth-tongue-sole-cynoglossus-semilaevis
#6
Zhenxia Sha, Linqing Wang, Luming Sun, Yadong Chen, Yuan Zheng, Mei Xin, Chen Li, Songlin Chen
In the present study, the peripheral blood cells of half smooth tongue sole (Cynoglossus semilaevis) were examined by blood smear under the light microscopy. The proportion of main types of blood cells are as following: erythrocyte occupied the majority (92.3%), followed by thrombocyte (4.15%), granulocyte (1.7%), lymphocyte (1.5%) and monocyte (0.3%), respectively. Meanwhile, the isolation method of monocytes was established, by density gradient centrifugation to isolate mononuclear leukocytes of peripheral blood...
April 19, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28429575/recovery-from-talaromyces-marneffei-involving-the-kidney-in-a-renal-transplant-recipient-a-case-report-and-literature-review
#7
Juan Peng, Zhiyong Chen, Ruiming Cai, Xianen Huang, Liying Lin, Weixiang Liang, Zhongtang Xiong, Juan Chen, Hui Chen, Yuexin Yang, Shaoyan Liu, Qingping Jiang
Talaromyces marneffei is an emerging opportunistic infection among immunocompromised patients. We observe the first native case of disseminated T. marneffei involving the kidney in a renal transplant recipient in mainland China. We describe the comprehensive clinical course, and ultrasound imaging of renal transplant biopsy, pathologic images, and electron microscopy observation of the biopsy specimen, highlighting the relevance of biopsy findings and the blood culture. We also focus on the treatment and good outcome of the patient...
April 21, 2017: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/28427796/molecular-methods-for-septicemia-diagnosis
#8
Francesc Marco
Septicemia remains a major cause of hospital mortality. Blood culture remains the best approach to identify the etiological microorganisms when a bloodstream infection is suspected but it takes long time because it relies on bacterial or fungal growth. The introduction in clinical microbiology laboratories of the matrix-assisted laser desorption ionization time-of-flight mass spectrometry technology, DNA hybridization, microarrays or rapid PCR-based test significantly reduce the time to results. Tests for direct detection in whole blood samples are highly desirable because of their potential to identify bloodstream pathogens without waiting for blood cultures to become positive...
April 17, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/28425643/chemical-activation-in-blood-serum-and-human-cell-culture-improved-ruthenium-complex-for-catalytic-uncaging-of-alloc-protected-amines
#9
Timo Völker, Eric Meggers
Chemical as opposed to light-induced activation of caged molecules is a rapidly advancing approach to trigger biological processes. We recently introduced a ruthenium-catalyzed release of alloc-protected amines in human cells (Angew. Chem. Int. Ed. 2006, 45, 5645 and 2014, 53, 10536). A restriction of this and all other methods reported to date is a limited lifetime of the catalyst which hampers meaningful applications. In this study we address this problem with the development of a new generation of ruthenium complexes for the uncaging of alloc-protected amines with superior catalytic activity...
April 20, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28423355/mesenchymal-stem-cells-release-exosomes-that-transfer-mirnas-to-endothelial-cells-and-promote-angiogenesis
#10
Min Gong, Bin Yu, Jingcai Wang, Yigang Wang, Min Liu, Christian Paul, Ronald W Millard, De-Sheng Xiao, Muhammad Ashraf, Meifeng Xu
Mesenchymal stem cells (MSCs) have been found to benefit patients with a variety of ischemic diseases via promoting angiogenesis. It is also well established that exosomes secreted from MSCs deliver bioactive molecules, including microRNAs (miRs) to recipient cells. Therefore, we hypothesized that exosomes secreted from MSCs deliver miRs into endothelial cells and mediate angiogenesis. The pro-angiogenic stimulatory capacity of exosomes was investigated using tube-like structure formation and spheroid-based sprouting of human umbilical vein endothelial cells (HUVECs), and in vivo Matrigel plug assay...
April 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28419136/occurrence-of-leishmania-infantum-in-the-central-nervous-system-of-naturally-infected-dogs-parasite-load-viability-co-infections-and-histological-alterations
#11
Valéria da Costa Oliveira, Viviane Cardoso Boechat, Artur Augusto Velho Mendes Junior, Maria de Fátima Madeira, Luiz Claudio Ferreira, Fabiano Borges Figueiredo, Monique Paiva Campos, Francisco das Chagas de Carvalho Rodrigues, Raquel de Vasconcellos Carvalhaes de Oliveira, Maria Regina Reis Amendoeira, Rodrigo Caldas Menezes
Zoonotic visceral leishmaniasis is caused by the protozoan Leishmania infantum and little is known about the occurrence and pathogenesis of this parasite in the CNS. The aims of this study were to evaluate the occurrence, viability and load of L. infantum in the CNS, and to identify the neurological histological alterations associated with this protozoan and its co-infections in naturally infected dogs. Forty-eight Leishmania-seropositive dogs from which L. infantum was isolated after necropsy were examined...
2017: PloS One
https://www.readbyqxmd.com/read/28410232/vacquinol-1-inducible-cell-death-in-glioblastoma-multiforme-is-counter-regulated-by-trpm7-activity-induced-by-exogenous-atp
#12
Philip Sander, Haouraa Mostafa, Ayman Soboh, Julian M Schneider, Andrej Pala, Ann-Kathrin Baron, Barbara Moepps, C Rainer Wirtz, Michael Georgieff, E Marion Schneider
Glioblastomas (GBM) are the most malignant brain tumors in humans and have a very poor prognosis. New therapeutic options are urgently needed. A novel drug, Vacquinol-1 (Vac), a quinolone derivative, displays promising properties by inducing rapid cell death in GBM but not in non-transformed tissues. Features of this type of cell death are compatible with a process termed methuosis. Here we tested Vac on a highly malignant glioma cell line observed by long-term video microscopy. Human dental-pulp stem cells (DPSCs) served as controls...
March 30, 2017: Oncotarget
https://www.readbyqxmd.com/read/28406949/antimicrobial-resistant-infections-among-postpartum-women-at-a-ugandan-referral-hospital
#13
Lisa M Bebell, Joseph Ngonzi, Joel Bazira, Yarine Fajardo, Adeline A Boatin, Mark J Siedner, Ingrid V Bassett, Dan Nyehangane, Deborah Nanjebe, Yves Jacquemyn, Jean-Pierre van Geertruyden, Juliet Mwanga-Amumpaire, David R Bangsberg, Laura E Riley, Yap Boum
INTRODUCTION: Puerperal sepsis causes 10% of maternal deaths in Africa, but prospective studies on incidence, microbiology and antimicrobial resistance are lacking. METHODS: We performed a prospective cohort study of 4,231 Ugandan women presenting to a regional referral hospital for delivery or postpartum care, measured vital signs after delivery, performed structured physical exam, symptom questionnaire, and microbiologic evaluation of febrile and hypothermic women...
2017: PloS One
https://www.readbyqxmd.com/read/28402318/memory-enhancing-effects-of-gebr-32a-a-new-pde4d-inhibitor-holding-promise-for-the-treatment-of-alzheimer-s-disease
#14
Roberta Ricciarelli, Chiara Brullo, Jos Prickaerts, Ottavio Arancio, Carla Villa, Claudia Rebosio, Elisa Calcagno, Matilde Balbi, Britt T J van Hagen, Elentina K Argyrousi, Hong Zhang, Maria Adelaide Pronzato, Olga Bruno, Ernesto Fedele
Memory loss characterizes several neurodegenerative disorders, including Alzheimer's disease (AD). Inhibition of type 4 phosphodiesterase (PDE4) and elevation of cyclic adenosine monophosphate (cAMP) has emerged as a promising therapeutic approach to treat cognitive deficits. However, PDE4 exists in several isoforms and pan inhibitors cannot be used in humans due to severe emesis. Here, we present GEBR-32a, a new PDE4D full inhibitor that has been characterized both in vitro and in vivo using biochemical, electrophysiological and behavioural analyses...
April 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28400735/a-novel-stopped-flow-assay-for-quantitating-carbonic-anhydrase-activity-and-assessing-red-blood-cell-hemolysis
#15
Pan Zhao, R Ryan Geyer, Walter F Boron
We report a novel carbonic-anhydrase (CA) assay and its use for quantitating red-blood-cell (RBC) lysis during stopped-flow (SF) experiments. We combine two saline solutions, one containing HEPES/pH 7.03 and the other, ~1% CO2/44 mM [Formula: see text]/pH 8.41, to generate an out-of-equilibrium CO2/[Formula: see text] solution containing ~0.5% CO2/22 [Formula: see text]/pH ~7.25 (10°C) in the SF reaction cell. CA catalyzes relaxation of extracellular pH to ~7.50: [Formula: see text] + H(+) → CO2 + H2O. Proof-of-concept studies (no intact RBCs) show that the pH-relaxation rate constant (kΔpH)-measured via pyranine fluorescence-rises linearly with increases in [bovine CAII] or [murine-RBC lysate]...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28400691/metastatic-endogenous-endophthalmitis-a-rare-presentation-with-methicillin-resistant-staphylococcus-aureus-prostatic-abscess
#16
Mrugank M Bhavsar, T V Devarajan, P Senthur Nembi, Nagarajan Ramakrishnan, Ashwin K Mani
A 62-year-old male with the previous history of uncontrolled diabetes and hypertension on irregular treatment presented with a history of fever, dysuria, and urinary retention with progressive painful loss of vision over a period of 2 days. His eye examination showed hypopyon, and he was diagnosed to have rapidly progressive endogenous endophthalmitis. He was started on vancomycin and piperacillin-tazobactam empirically. His blood and urine cultures grew methicillin-resistant Staphylococcus aureus (MRSA). Transesophageal echocardiography ruled out infective endocarditis...
March 2017: Indian Journal of Critical Care Medicine
https://www.readbyqxmd.com/read/28397475/evaluation-of-maldi-tof-ms-for-the-identification-of-yeast-isolates-causing-bloodstream-infection
#17
Ozge Turhan, Betil Ozhak-Baysan, Oscar Zaragoza Hernandez, Halil Er, Gozde Ongut, Dilara Ogunc, Dilek Colak, Manuel Cuenca-Estrella
BACKGROUND: Infections due to Candida species are major causes of morbidity and mortality in humans, causing a diverse spectrum of clinical disease ranging from superficial and mucosal infections to invasive disease. Several authors have demonstrated that mortality is closely linked to both timing of therapy and/or source control. The rapid identification of pathogenic species is helpful to start timely and effective antifungal therapy. The aim of this study was to assess the performance of the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system for the correct and rapid identification of yeast isolates causing bloodstream infection...
April 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28397470/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms-for-early-identification-of-septic-patients
#18
Ali O Sekercioglu, Yesim Cekin, Dilara Ogunc, Gozde Ongut, Betil O Baysan, Dilek Colak, Filiz Gunseren, Levent Donmez
BACKGROUND: Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a powerful technique for the rapid identification of bacteria from growing colonies in routine cultures. In this study, we evaluated the feasibility of a 5-hour incubation on solid medium after sub-cultivation of positive blood culture broth without any preparation steps in order to speed up the identification of bacteria. METHODS: In addition to standard laboratory protocols, a Columbia agar plate with 5% sheep blood was inoculated with 1 drop from the blood culture broth...
April 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28397101/direct-detection-of-extended-spectrum-beta-lactamases-ctx-m-from-blood-cultures-by-lc-ms-ms-bottom-up-proteomics
#19
F Fleurbaaij, W Goessens, H C van Leeuwen, M E M Kraakman, S T Bernards, P J Hensbergen, E J Kuijper
Rapid bacterial species identification and antibiotic susceptibility testing in positive blood cultures have an important impact on the antibiotic treatment for patients. To identify extended-spectrum beta-lactamases (ESBL) directly in positive blood culture bottles, we developed a workflow of saponin extraction followed by a bottom-up proteomics approach using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The workflow was applied to positive blood cultures with Escherichia coli and Klebsiella pneumoniae collected prospectively in two academic hospitals over a 4-month period...
April 10, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28392378/transplantation-of-ex-vivo-expanded-umbilical-cord-blood-nicord-decreases-early-infection-and-hospitalization
#20
Sarah Anand, Samantha Thomas, Terry Hyslop, Janet Adcock, Kelly Corbet, Cristina Gasparetto, Richard Lopez, Gwynn D Long, Ashley K Morris, David A Rizzieri, Keith M Sullivan, Anthony D Sung, Stefanie Sarantopoulos, Nelson J Chao, Mitchell E Horwitz
Delayed hematopoietic recovery contributes to increased infection risk following umbilical cord blood (UCB) transplantation. In a Phase 1 study, adult recipients of UCB stem cells cultured ex vivo for 3 weeks with nicotinamide (NiCord) had earlier median neutrophil recovery compared to historical controls. To evaluate the impact of faster neutrophil recovery on clinically relevant early outcomes, we reviewed infection episodes and hospitalization during the first 100 days in an enlarged cohort of 18 NiCord recipients compared to 86 standard UCB recipients at our institution...
April 6, 2017: Biology of Blood and Marrow Transplantation
keyword
keyword
119135
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"