keyword
MENU ▼
Read by QxMD icon Read
search

Rapid blood culture

keyword
https://www.readbyqxmd.com/read/29142077/syndromic-panel-based-testing-in-clinical-microbiology
#1
REVIEW
Poornima Ramanan, Alexandra L Bryson, Matthew J Binnicker, Bobbi S Pritt, Robin Patel
The recent development of commercial panel-based molecular diagnostics for the rapid detection of pathogens in positive blood culture bottles, respiratory specimens, stool, and cerebrospinal fluid has resulted in a paradigm shift in clinical microbiology and clinical practice. This review focuses on U.S. Food and Drug Administration (FDA)-approved/cleared multiplex molecular panels with more than five targets designed to assist in the diagnosis of bloodstream, respiratory tract, gastrointestinal, or central nervous system infections...
January 2018: Clinical Microbiology Reviews
https://www.readbyqxmd.com/read/29140934/response-of-symptomatic-persistent-chronic-disseminated-candidiasis-to-corticosteroid-therapy-in-immunosuppressed-pediatric-patients-case-study-and-review-of-the-literature
#2
Vered Shkalim-Zemer, Itzhak Levi, Salvador Fischer, Hannah Tamary, Joanne Yakobovich, Gali Avrahami, Gil Gilad, Sara Elitzur, Isaac Yaniv, Ronit Elhasid, Michal Manistersky, Itamar Shalit
BACKGROUND: Chronic disseminated candidiasis (CDC) is a severe invasive fungal infection principally observed during neutrophil recovery in patients with acute leukemia treated with intensive chemotherapy. Its pathophysiology remains unclear. We describe the management of six children with symptomatic CDC who did not respond to antifungal therapy. METHODS: The databases of the hematology-oncology departments of two tertiary pediatric medical centers were searched for all patients diagnosed with CDC from 2003 to 2015 who responded to corticosteroids after failing antifungal therapy...
November 14, 2017: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/29126269/evaluation-of-the-kpc-k-set%C3%A2-immunochromatographic-assay-for-the-rapid-detection-of-kpc-carbapenemase-producers-from-positive-blood-cultures
#3
Eleonora Riccobono, Alberto Antonelli, Patrizia Pecile, Pierre Bogaerts, Marco Maria D'Andrea, Gian Maria Rossolini
No abstract text is available yet for this article.
November 8, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29122896/persistent-bacteraemia-caused-by-staphylococcus-aureus-in-the-gall-bladder
#4
Alexander Tin Han Yu, Tony Cun, Esther Benamu, Cybele Renault
Staphylococcus aureusbacteraemia (SAB) remains a complex disease with a high associated morbidity and mortality, especially when it is able to establish an occult nidus safe from antimicrobial eradication. Without rapid identification and intervention, the nidus can cause persistent relapse of disease, morbidity and mortality. Having a high clinical suspicion for the foci of occult S. aureus is important, and awareness of potential sites of infection is critical and can be life-saving.We present a unique case of a 65-year-old man with end-stage renal disease receiving haemodialysis who developed septic shock from SAB...
November 8, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/29119674/assessment-of-hydrogels-for-bioprinting-of-endothelial-cells
#5
Leo Benning, Ludwig Gutzweiler, Kevin Tröndle, Julian Riba, Roland Zengerle, Peter Koltay, Stefan Zimmermann, G Björn Stark, Günter Finkenzeller
In tissue engineering applications, vascularization can be accomplished by co-implantation of tissue forming cells and endothelial cells (ECs), whereby the latter are able to form functional blood vessels. The use of three-dimensional (3D) bioprinting technologies has the potential to improve the classical tissue engineering approach because these will allow the generation of scaffolds with high spatial control of endothelial cell allocation. This study focuses on a side by side comparisons of popular commercially available bioprinting hydrogels (matrigel, fibrin, collagen, gelatin, agarose, Pluronic F-127, alginate and alginate/gelatin) in the context of their physicochemical parameters, their swelling/degradation characteristics, their biological effects on vasculogenesis-related EC parameters and their printability...
November 8, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29118168/use-of-accelerate-pheno%C3%A2-system-for-identification-and-antimicrobial-susceptibility-testing-id-ast-of-pathogens-in-positive-blood-cultures-and-impact-on-time-to-results-and-workflow
#6
Angella Charnot-Katsikas, Vera Tesic, Nedra Love, Brandy Hill, Cindy Bethel, Sue Boonlayangoor, Kathleen G Beavis
The Accelerate Pheno™ system uses automated fluorescence in situ hybridization technology with morphokinetic cellular analysis to provide rapid species identification (ID) and antimicrobial susceptibility testing (AST) results for the most commonly identified organisms in bloodstream infections.Objective was to evaluate the accuracy and workflow of bacterial and yeast ID, and bacterial AST using the Accelerate Pheno™ system in the clinical microbiology laboratory.Consecutive fresh blood cultures received in the laboratory were analyzed by the Accelerate Pheno™ system within 0 - 8 h of growth detection...
November 8, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29117261/diagnostic-accuracy-of-the-roche-septifast-pcr-system-for-the-rapid-detection-of-blood-pathogens-in-neonatal-sepsis-a-prospective-clinical-trial
#7
Julia Straub, Helga Paula, Michaela Mayr, David Kasper, Ojan Assadian, Angelika Berger, Judith Rittenschober-Böhm
INTRODUCTION: Diagnosis of neonatal sepsis remains a major challenge in neonatology. Most molecular-based methods are not customized for neonatal requirements. The aim of the present study was to assess the diagnostic accuracy of a modified multiplex PCR protocol for the detection of neonatal sepsis using small blood volumes. METHODS: 212 episodes of suspected neonatal late onset sepsis were analyzed prospectively using the Roche SeptiFast® MGRADE PCR with a modified DNA extraction protocol and software-handling tool...
2017: PloS One
https://www.readbyqxmd.com/read/29111432/direct-determination-of-carbapenem-resistant-enterobacteriaceae-and-pseudomonas-aeruginosa-from-positive-blood-cultures-using-laser-scattering-technology
#8
Evgeny A Idelevich, Matthias Hoy, Dennis Knaack, Dennis Görlich, Georg Peters, Matthias Borowski, Karsten Becker
OBJECTIVE: Delays in appropriate antimicrobial treatment contribute to increased mortality of septic patients. We aimed to develop a methodology for detection of carbapenem resistance in Gram-negative bacteria directly from positive blood cultures (BCs). METHODS: Initially, meropenem-resistant Enterobacteriaceae (n=13) and Pseudomonas aeruginosa (n=32) isolates as well as the same numbers of meropenem-susceptible isolates were used to establish the detection of carbapenem resistance from agar cultures...
October 27, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29110928/direct-bacterial-identification-from-positive-blood-cultures-using-matrix-assisted-laser-desorption-ionization-time-of-flight-maldi-tof-mass-spectrometry-a-systematic-review-and-meta-analysis
#9
Jesús Ruiz-Aragón, Mónica Ballestero-Téllez, Belén Gutiérrez-Gutiérrez, Marina de Cueto, Jesús Rodríguez-Baño, Álvaro Pascual
INTRODUCTION: The rapid identification of bacteraemia-causing pathogens could assist clinicians in the timely prescription of targeted therapy, thereby reducing the morbidity and mortality of this infection. In recent years, numerous techniques that rapidly and directly identify positive blood cultures have been marketed, with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) being one of the most commonly used. METHODS: The aim of this systematic review and meta-analysis was to evaluate the accuracy of MALDI-TOF (Bruker(®)) for the direct identification of positive blood culture bottles...
October 27, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/29105868/successful-treatment-of-disseminated-nocardiosis-caused-by-nocardia-veterana-in-a-dog
#10
S Yaemsiri, J E Sykes
A 5-year-old male castrated Lhasa Apso cross was evaluated for a 1-month history of inappetence, lethargy, gagging, and progressive right thoracic limb lameness. Synovial fluid analysis revealed nonseptic suppurative inflammation, and a diagnosis of immune-mediated polyarthritis (IMPA) was made. After 3 months of treatment with prednisone and later cyclosporine, the dog developed multiple firm cutaneous and subcutaneous masses and a focal mass within the jejunum. Cultures of blood, urine, skin lesions, and the jejunal mass identified Nocardia veterana by matrix-absorption laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and allowed for earlier identification of the organism compared to more traditional secA1 gene sequencing...
November 4, 2017: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/29100993/hepatitis-c-virus-induced-natural-killer-cell-proliferation-involves-monocyte-derived-cells-and-the-ox40-ox40l-axis
#11
Julia Pollmann, Jana-Julia Götz, Daniel Rupp, Otto Strauss, Markus Granzin, Oliver Grünvogel, Pascal Mutz, Catharina Kramer, Felix Lasitschka, Volker Lohmann, Niklas K Björkström, Robert Thimme, Ralf Bartenschlager, Adelheid Cerwenka
BACKGROUND AND AIMS: Natural killer (NK) cells are found at increased frequencies in hepatitis C virus (HCV)-infected patients. NK cell activation was shown to correlate with HCV clearance and to predict favourable treatment response. The aim of our study was to dissect mechanisms leading to NK cell activation and proliferation in response to HCV. METHODS: NK cell phenotype, proliferation, and function were assessed after six-day co-culture of human peripheral blood mononuclear cells (PBMCs) with HCV-replicon containing Huh6 hepatoblastoma cells or HCV-infected Huh7...
October 31, 2017: Journal of Hepatology
https://www.readbyqxmd.com/read/29100506/long-term-in-vitro-culture-of-plasmodium-vivax-isolates-from-madagascar-maintained-in-saimiri-boliviensis-blood
#12
Rajeev K Mehlotra, D'Arbra Blankenship, Rosalind E Howes, Tovonahary A Rakotomanga, Brune Ramiranirina, Stephanie Ramboarina, Thierry Franchard, Marlin H Linger, Melinda Zikursh-Blood, Arsène C Ratsimbasoa, Peter A Zimmerman, Brian T Grimberg
BACKGROUND: Plasmodium vivax is the most prevalent human malaria parasite and is likely to increase proportionally as malaria control efforts more rapidly impact the prevalence of Plasmodium falciparum. Despite the prominence of P. vivax as a major human pathogen, vivax malaria qualifies as a neglected and under-studied tropical disease. Significant challenges bringing P. vivax into the laboratory, particularly the capacity for long-term propagation of well-characterized strains, have limited the study of this parasite's red blood cell (RBC) invasion mechanism, blood-stage development, gene expression, and genetic manipulation...
November 3, 2017: Malaria Journal
https://www.readbyqxmd.com/read/29091139/glutaraldehyde-test-for-the-rapid-diagnosis-of-pulmonary-and-extra-pulmonary-tuberculosis-in-an-area-with-high-tuberculosis-incidence
#13
Ben Hadj Hassine Ahmed, Marzouk Manel, Dhaou Mohamed, Boukadida Jalel
BACKGROUND: Tuberculosis (TB) remains one of the leading causes of morbidity and mortality worldwide. The primary method for controlling TB is the rapid and accurate identification of infected individuals. Immune response exploitation represents one of the main methods used for early TB diagnosis; however, few studies have reported that whole blood originating from TB-infected patients gels faster in the presence of aldehyde than blood originating from healthy subjects, which is the focus of the current study...
November 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/29070795/ecobody-technology-rapid-monoclonal-antibody-screening-method-from-single-b-cells-using-cell-free-protein-synthesis-for-antigen-binding-fragment-formation
#14
Teruyo Ojima-Kato, Satomi Nagai, Hideo Nakano
We report a rapid and cost-effective monoclonal antibody screening method from single animal B cells using reverse transcription (RT)-PCR and Escherichia coli cell-free protein synthesis (CFPS), which allows evaluation of antibodies within 2 working days. This process is named "Ecobody technology". The method includes strategies to isolate B cells that specifically bind an antigen from the peripheral blood of immunised animals, and single-cell RT-PCR to generate DNA fragments of the VH and VL genes, followed by CFPS for production of fragments of antigen binding (Fab)...
October 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29067121/effect-of-testosterone-and-hypoxia-on-the-expansion-of-umbilical-cord-blood-cd34-cells-in-vitro
#15
Liping Zhou, Xiaowei Zhang, Panpan Zhou, Xue Li, Xuejing Xu, Qing Shi, Dong Li, Xiuli Ju
Successfully expanding hematopoietic stem cells (HSCs) is advantageous for clinical HSC transplantation. The present study investigated the influence of testosterone on the proliferation, antigen phenotype and expression of hematopoiesis-related genes in umbilical cord blood-derived cluster of differentiation (CD)34(+) cells under normoxic or hypoxia conditions. Cord blood (CB) CD34(+) cells were separated using magnetic activated cell sorting. A cytokine cocktail and feeder cells were used to stimulate the expansion of CD34(+) cells under normoxic (20% O2) and hypoxic (1% O2) conditions for 7 days and testosterone was added accordingly...
November 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29063883/standardization-of-a-two-step-real-time-polymerase-chain-reaction-based-method-for-species-specific-detection-of-medically-important-aspergillus-species
#16
P Das, P Pandey, A Harishankar, M Chandy, S Bhattacharya, A Chakrabarti
PURPOSE: Standardization of Aspergillus polymerase chain reaction (PCR) poses two technical challenges (a) standardization of DNA extraction, (b) optimization of PCR against various medically important Aspergillus species. Many cases of aspergillosis go undiagnosed because of relative insensitivity of conventional diagnostic methods such as microscopy, culture or antigen detection. The present study is an attempt to standardize real-time PCR assay for rapid sensitive and specific detection of Aspergillus DNA in EDTA whole blood...
July 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29062709/encapsulated-pleural-effusion-due-to-haemophilus-influenzae-biotype-ii-in-a-child-with-trisomy-21-a-case-report-and-literature-review
#17
Masanori Kaneko, Yuki Bando, Tomohiro Fujita, Yoneji Hirose, Eisuke Suganuma, Masahiro Ishii, Takashi Takahashi
Haemophilus influenzae (Hi) can colonize in the upper respiratory tract and cause severe pulmonary infections, especially among immunocompromised children. Herein, we report a case of left encapsulated pleural effusion (EPE) due to Hi in a 24-month-old girl with trisomy 21. She was already vaccinated against Hi type b. The Hi biotype II was isolated from both the blood and aspirated sputum obtained upon admission. Ampicillin/sulbactam 180 mg/kg/day was administered intravenously for 34 days with oxygen supplementation for 4 days...
2017: IDCases
https://www.readbyqxmd.com/read/29059213/endothelial-camkii-as-a-regulator-of-enos-activity-and-no-mediated-vasoreactivity
#18
Shubha Murthy, Olha M Koval, Juan M Ramiro Diaz, Santosh Kumar, Daniel Nuno, Jason A Scott, Chantal Allamargot, Linda J Zhu, Kim Broadhurst, Velarchana Santhana, William J Kutschke, Kaikobad Irani, Kathryn G Lamping, Isabella M Grumbach
The multifunctional Ca2+/calmodulin-dependent protein kinase II (CaMKII) is a serine/threonine kinase important in transducing intracellular Ca2+ signals. While in vitro data regarding the role of CaMKII in the regulation of endothelial nitric oxide synthase (eNOS) are contradictory, its role in endothelial function in vivo remains unknown. Using two novel transgenic models to express CaMKII inhibitor peptides selectively in endothelium, we examined the effect of CaMKII on eNOS activation, NO production, vasomotor tone and blood pressure...
2017: PloS One
https://www.readbyqxmd.com/read/29055117/can-neutrophil-to-lymphocyte-ratio-predict-late-onset-sepsis-in-preterm-infants
#19
Senem Alkan Ozdemir, Esra Arun Ozer, Ozkan Ilhan, Sumer Sutcuoglu
BACKGROUND: The neutrophil to lymphocyte ratio (NLR) is an easily accessible biomarker that has been reported to represent disease severity in adult trials. The aim of this study was to evaluate the relationship between culture positiveness and NLR in cases where the reason of sepsis was considered, and to foresight an idea about the active agents. METHODS: Preterm infants with birth weights ≤1500 g and/or ≤32 gestational weeks were eligible for this study...
October 21, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29054893/purpura-fulminans-a-rare-presentation-of-streptococcus-pneumoniae-infection
#20
Hock Gin Teo, Jun Yet Wong, Tracy Lee Lyee Ting
A previously healthy man presented with fever for 2 days and rapidly progressive purpuric rash for 1 day. He progressed into hypotension, disseminated intravascular coagulation and refractory shock despite resuscitation and early antibiotic commencement. Blood culture grew Streptococcus pneumoniae This case report highlights the fact that purpura fulminans can be a rare presentation of S. pneumoniae infection as well.
October 20, 2017: BMJ Case Reports
keyword
keyword
119135
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"