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Rapid blood culture

Dao-Ming Zhu, Lei Wu, Meng Suo, Song Gao, Wei Xie, Ming-Hui Zan, Ao Liu, Bei Chen, Wen-Tao Wu, Li-Wei Ji, Li-Ben Chen, Hui-Ming Huang, Shi-Shang Guo, Wen-Feng Zhang, Xing-Zhong Zhao, Zhi-Jun Sun, Wei Liu
Filtration of circulating tumor cells (CTCs) in peripheral blood is of proven importance for early cancer diagnosis, treatment monitoring, metastasis diagnosis, and prognostic evaluation. However, currently available strategies for enriching CTCs, such as magnetic activated cell sorting (MACS), face serious problems with purity due to nonspecific interactions between beads and leukocytes in the process of capturing. In the present study, the tumor-targeting molecule folic acid (FA) and magnetic nanoparticles (MNPs) were coated on the surface of red blood cells (RBCs) by hydrophobic interaction and chemical conjugation, respectively...
March 15, 2018: Nanoscale
Nicole M Bzdyl, Nadezda Urosevic, Ben Payne, Ray Brockenshire, Michael McIntyre, Michael J Leung, Graham Weaire-Buchanan, Elizabeth Geelhoed, Timothy J J Inglis
PURPOSE: In this field trial of rapid blood culture identification (BCID), we aimed to determine whether the improved speed and accuracy of specific BCID predicted in our earlier pilot study could be obtained in regional hospitals by deploying a multiplex PCR FilmArray (Biomerieux, France) capability in their laboratories. METHODS: We trained local hospital laboratory staff to operate the FilmArray equipment and act on the results. To do this, we integrated the multiplex PCR into the standard laboratory blood culture workflow and reporting procedure...
March 13, 2018: Journal of Medical Microbiology
Valentina Coccè, Anna Brini, Aldo Bruno Giannì, Valeria Sordi, Angiola Berenzi, Giulio Alessandri, Carlo Tremolada, Silvia Versari, Antonio Bosetto, Augusto Pessina
The adipose tissue is a good source of mesenchymal stromal cells that requires minimally invasive isolation procedures. To ensure reproducibility, efficacy, and safety for clinical uses, these procedures have to be in compliant with good manufacturing practices. Techniques for harvesting and processing human adipose tissue have rapidly evolved in the last years, and Lipogems® represents an innovative approach to obtain microfragmented adipose tissue in a short time, without expansion and/or enzymatic treatment...
2018: Stem Cells International
Annie W T Lee, Johnson K S Lam, Ricky K W Lam, Wan H Ng, Ella N L Lee, Vicky T Y Lee, Po P Sze, Rahim Rajwani, Kitty S C Fung, Wing K To, Rodney A Lee, Dominic N C Tsang, Gilman K H Siu
Objective: This study evaluated the capability of a MALDI Biotyper system equipped with the newly introduced MBT STAR-BL module to simultaneously perform species identification and β-lactamase-mediated resistance detection in bacteremia -causing bacteria isolated from cultured isolates and patient-derived blood cultures (BCs). Methods: Two hundred retrospective cultured isolates and 153 prospective BCs containing Gram-negative rods (GNR) were collected and subjected to direct bacterial identification, followed by the measurement of β-lactamase activities against ampicillin, piperacillin, cefotaxime, ceftazidime, and meropenem using the MBT STAR-BL module...
2018: Frontiers in Microbiology
Shang-Yu Chen, Tzu-Hua Weng, Wen-Pin Tseng, Chia-Ming Fu, Hui-Wen Lin, Chun-Hsing Liao, Tai-Fen Lee, Po-Ren Hsueh, Cheng-Chung Fang, Shey-Ying Chen
Few studies analyzed the association between blood culture time to positivity (TTP) and risk of complicated nontyphoidal Salmonella (NTS) bacteremia. We conducted a retrospective study of 206 patients (aged 60.4 ± 17.4 years) with NTS bacteremia during a 30-month period. Complicated NTS bacteremia was defined as the presence of 30-day mortality, complicated infection requiring surgery or abscess drainage, or requirement of intensive care unit admission. Serogroup D (75.7%) was the predominant isolates. Malignancy (44...
February 13, 2018: Diagnostic Microbiology and Infectious Disease
Maria Cryskely Agra Batinga, Julia Teresa Ribeiro de Lima, Fabio Gregori, Jaqueline Assumpção Diniz, Kerstin Muner, Trícia M F S Oliveira, Helena Lage Ferreira, Rodrigo Martins Soares, Lara Borges Keid
Canine brucellosis is caused by Brucella canis, a gram negative and facultative intracellular bacterium that is commonly associated with reproductive failures in dogs. The accurate diagnosis of the infection relies on the use of serological tests associated with blood culturing to guarantee sensitivity. The polymerase chain reaction (PCR) can replace the culturing procedure for the direct diagnosis of the infection because of its speed, high specificity and sensitivity values; however, it depends on some laboratory infrastructure to be conducted...
March 7, 2018: Molecular and Cellular Probes
Laura M F Kuijpers, Panha Chung, Marjan Peeters, Marie-France Phoba, Chun Kham, Barbara Barbé, Octavie Lunguya, Jan Jacobs
BACKGROUND: In low resource settings, Salmonella serovars frequently cause bloodstream infections. This study investigated the diagnostic performance of immunochromatographic rapid diagnostic tests (RDTs), which detect Salmonella antigens, when applied to stored grown blood culture broth. MATERIAL/METHODS: The SD Bioline One Step Salmonella Typhi Ag Rapid Detection Kit (Standard Diagnostics, Republic of Korea), marketed for the detection of Salmonella enterica serovar Typhi (Salmonella Typhi) in stool and the Salmonella Ag Rapid Test (Creative Diagnostics, USA), marketed for the detection of all Salmonella serotypes in stool, were selected for evaluation based on a pre-test evaluation of six RDT products...
2018: PloS One
Hailian Wang, Wei Ge, Yong Zhuang, Jinqiu Fu, Dong Li, Xiuli Ju
Background: Cord blood transplantation (CBT) can be a life-saving procedure in the treatment of a broad variety of disorders, including hematologic, immune, and genetic diseases. However, delayed platelet recovery hinders the application of CBT. Purpose: The aim of this study was to determine the optimal combination of cytokines to amplify megakaryocyte (Mk). Methods: CB CD34+ cells were obtained by immunomagnetic isolation and amplified under four different cytokine combinations...
January 2018: Journal of Cancer Research and Therapeutics
Patricia M Spittal, Samuel S Malamba, Martin D Ogwang, Seggane Musisi, J Paul Ekwaru, Nelson K Sewankambo, Margo E Pearce, Kate Jongbloed, Sheetal H Patel, Achilles Katamba, Alden H Blair, Herbert Muyinda, Martin T Schechter Obc
BACKGROUND: As previously encamped resettle, potential for rapid HIV transmission in post-conflict Northern Uganda is concerning. Women in particular may be experiencing heightened vulnerability resulting from war-related sexual violence. SETTING: Cango Lyec (Healing the Elephant) Project is a cohort involving conflict-affected people in three districts in Northern Uganda. METHODS: Eight randomly selected communities were mapped and a census was conducted...
March 2, 2018: Journal of Acquired Immune Deficiency Syndromes: JAIDS
Brigitte Lamy, Martin Sundqvist
This editorial is aimed at presenting the theme issue entitled "Improving the Diagnosis of Bloodstream Infections - Advancing Technology and Quality for Better Care", and is designed to present several aspects of of blood culture improvement, from prevention of contaminants to quality assurance, rapid methods implementation and unsolved issues.
February 28, 2018: Clinical Microbiology and Infection
Xinjie Zhang, Zhixian Zhu, Nan Xiang, Feifei Long, Zhonghua Ni
Microfluidic technologies for cell separation were reported frequently in recent years. However, compact microfluidic instrument enabling thoroughly automated cell separation is still rarely reported until today due to difficult hybrid between macro-sized fluidic control system and micro-sized microfluidic device. In this work, we propose a novel and automated microfluidic instrument to realize size-based separation of cancer cells in label-free and high-throughput manner. Briefly, the instrument is equipped with a fully integrated microfluidic device and a set of robust fluid-driven and control units, and the instrument functions of precise fluid infusion and high-throughput cell separation are guaranteed by a flow regulatory chip and two cell separation chips which are the key components of the microfluidic device...
March 1, 2018: Analytical Chemistry
Mridu Sinha, Julietta Jupe, Hannah Mack, Todd P Coleman, Shelley M Lawrence, Stephanie I Fraley
Rapid and accurate profiling of infection-causing pathogens remains a significant challenge in modern health care. Despite advances in molecular diagnostic techniques, blood culture analysis remains the gold standard for diagnosing sepsis. However, this method is too slow and cumbersome to significantly influence the initial management of patients. The swift initiation of precise and targeted antibiotic therapies depends on the ability of a sepsis diagnostic test to capture clinically relevant organisms along with antimicrobial resistance within 1 to 3 h...
April 2018: Clinical Microbiology Reviews
N D B Ehelepola, R K G M Rajapaksha, D M U B Dhanapala, T D K Thennekoon, S Ponnamperuma
BACKGROUND: Concurrent presence of dengue hemorrhagic fever (DHF), tropical pyomyositis and septicemia due to methicillin-resistant Staphylococcus aureus (MRSA) in a previously healthy person has never been reported. These three conditions even individually are potentially fatal. "Here we describe a case of a patient contracting dengue and developing DHF along with concurrent pyomyositis likely to be due to MRSA, leading to MRSA septicemia with abscesses formed by MRSA". CASE PRESENTATION: A 44-year old previously healthy Sinhalese man presented on day 3 of the illness with fever, headache, arthralgia and myalgia and watery loose stools...
February 27, 2018: BMC Infectious Diseases
Yan-Hong Yong, Shao-Feng Liu, Guo-Hong Hua, Ru-Min Jia, Ravi Gooneratne, Yun-Tao Zhao, Ming Liao, Xiang-Hong Ju
Induction of the innate immune pathways is critical for early anti-viral defense. How geese recognize viral molecules and activate these pathways is not well understood. In mammals, Toll-like receptor 3 (TLR3) recognizes double-stranded RNA. Activation of TLR3 induces the activation of NF-кB and the production of type-I interferon. In this study, the goose TLR3 gene was cloned using rapid amplification of cDNA ends. Goose TLR3 encoded an 896-amino-acid protein, containing a signal secretion peptide, 14 extracellular leucine-rich repeat domains, a transmembrane domain, a Toll/interleukin-1 receptor signaling domain, and shared 46...
March 2018: Veterinary Immunology and Immunopathology
Alix Pantel, Julien Monier, Jean-Philippe Lavigne
Objectives: To evaluate the performance of the Accelerate Pheno™ system for the identification and antimicrobial susceptibility testing (AST) of a panel of Gram-negative bacilli (GNB) with different resistance profiles (e.g. penicillinases, ESBLs, cephalosporinase overproduction, carbapenemases, impermeability) directly from positive blood cultures in <7 h. Methods: A panel of 105 clinical strains previously characterized for the presence of β-lactamase-encoding genes was tested...
February 20, 2018: Journal of Antimicrobial Chemotherapy
Julie Denis, Faezeh Forouzanfar, Raoul Herbrecht, Elise Toussaint, Romain Kessler, Marcela Sabou, Ermanno Candolfi, Valérie Letsher-Bru
Invasive pulmonary aspergillosis (IPA) is a common complication of immunosuppression. Rapid diagnosis using molecular techniques is essential to improve patient survival. PCR techniques are promising in enhancing Aspergillus detection in blood and respiratory samples. Here, we evaluate for the first time the performances of two commercial Real-Time PCR kits, the A. fumigatus® Bio-Evolution and the MycoGENIE® A. fumigatus for the detection of A. fumigatus DNA in broncho-alveolar lavage (BAL) from patients with and without IPA...
February 19, 2018: Journal of Molecular Diagnostics: JMD
Filip Roudnicky, Sun Young Yoon, Susanna Poghosyan, Simon Schwager, Cedric Poyet, Giorgia Vella, Samia B Bachmann, Sinem Karaman, Jay W Shin, Vivianne I Otto, Michael Detmar
Thrombospondin-2 (TSP2) is an anti-angiogenic matricellular protein that inhibits tumor growth and angiogenesis. Tumor-associated blood vascular endothelial cells (BECs) were isolated from human invasive bladder cancers and from matched normal bladder tissue by immuno-laser capture microdissection. Exon expression profiling analyses revealed a particularly high expression of a short TSP2 transcript containing only the last 9 (3') exons of the full-length TSP2 transcript. Using 5' and 3' RACE (rapid amplification of cDNA ends) and Sanger sequencing, we confirmed the existence of the shorter transcript of TSP2 (sTSP2) and determined its sequence which completely lacked the anti-angiogenic thrombospondin type 1 repeats domain...
February 22, 2018: Oncogene
Xuyang Shi, Usha Kadiyala, J Scott VanEpps, Siu-Tung Yau
The current culture-based approach for the diagnosis of bloodstreams infection is incommensurate with timely treatment and curbing the prevalence of multi-drug resistant organisms (MDROs) due to its long time-to-result. Bloodstream infections typically involve extremely low (e.g., <10 colony-forming unit (CFU)/mL) bacterial concentrations that require a labor-intensive process and as much as 72 hours to yield a diagnosis. Here, we demonstrate a culture-free approach to achieve rapid diagnosis of bloodstream infections...
February 21, 2018: Scientific Reports
Luke W Anson, Kevin Chau, Nicholas Sanderson, Sarah Hoosdally, Phelim Bradley, Zamin Iqbal, Hang Phan, Dona Foster, Sarah Oakley, Marcus Morgan, Tim E A Peto, Modernizing Medical Microbiology Informatics Group Mmmig, Derrick W Crook, Louise J Pankhurst
PURPOSE: Speed of bloodstream infection diagnosis is vital to reduce morbidity and mortality. Whole genome sequencing (WGS) performed directly from liquid blood culture could provide single-assay species and antibiotic susceptibility prediction; however, high inhibitor and human cell/DNA concentrations limit pathogen recovery. We develop a method for the preparation of bacterial DNA for WGS-based diagnostics direct from liquid blood culture. METHODOLOGY: We evaluate three commercial DNA extraction kits: BiOstic Bacteraemia, Amplex Hyplex and MolYsis Plus...
March 2018: Journal of Medical Microbiology
Jeong-Han Kim, Taek Soo Kim, Sang Hoon Song, Jungil Choi, Sangkwon Han, Dong Young Kim, Sunghoon Kwon, Eunyoung Lee, Kyoung-Ho Song, Pyeong Gyun Choe, Ji Hwan Bang, Eu Suk Kim, Sang Won Park, Hong Bin Kim, Nam Joong Kim, Wan Beom Park, Myoung-Don Oh
PURPOSE: The direct rapid antibiotic susceptibility test (dRAST), based on analysing changes in bacterial micro-colonies under antibiotic conditions, detects antibiotic resistance within 6 h of direct smear examination results. This study aimed to assess the accuracy of dRAST and evaluate its potential usefulness for improving selection of appropriate antibiotic in real clinical practice settings. METHODOLOGY: We evaluated the accuracy of dRAST by comparing the antibiotic treatments that should have been administered based on dRAST results and the broth microdilution (BMD) test and its potential usefulness via simulation...
January 12, 2018: Journal of Medical Microbiology
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