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citrate longing solution

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https://www.readbyqxmd.com/read/27865859/solubilization-of-proteins-in-aqueous-two-phase-extraction-through-combinations-of-phase-formers-and-displacement-agents
#1
Christian Kress, Gabriele Sadowski, Christoph Brandenbusch
The aqueous two-phase extraction (ATPE) of therapeutic proteins is a promising separation alternative to cost-intensive chromatography, still being the workhorse of nowadays downstream processing. As shown in many publications, using NaCl as displacement agent in salt-polymer ATPE allows for a selective purification of the target protein immunoglobulin G (IgG) from human serum albumin (HSA, represents the impurity). However a high yield of the target protein is only achievable as long as the protein is stabilized in solution and not precipitated...
November 17, 2016: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/27844288/methylphenidate-decreases-atp-levels-and-impairs-glutamate-uptake-and-na-k-atpase-activity-in-juvenile-rat-hippocampus
#2
Felipe Schmitz, Paula Pierozan, André F Rodrigues, Helena Biasibetti, Mateus Grings, Bruna Zanotto, Daniella M Coelho, Carmen R Vargas, Guilhian Leipnitz, Angela T S Wyse
The study of the long-term neurological consequences of early exposure with methylphenidate (MPH) is very important since this psychostimulant has been widely misused by children and adolescents who do not meet full diagnostic criteria for ADHD. The aim of this study was to examine the effect of early chronic exposure with MPH on amino acids profile, glutamatergic and Na(+),K(+)-ATPase homeostasis, as well as redox and energy status in the hippocampus of juvenile rats. Wistar male rats received intraperitoneal injections of MPH (2...
November 14, 2016: Molecular Neurobiology
https://www.readbyqxmd.com/read/27823671/in-situ-hydrazine-reduced-silver-colloid-synthesis-enhancing-sers-reproducibility
#3
Vera Dugandžić, Izabella Jolan Hidi, Karina Weber, Dana Cialla-May, Jürgen Popp
Herein we report a novel strategy for the in situ synthesis of the silver colloids for LoC-SERS applications. Silver nanoparticles are obtained in a segmented flow based glass microfluidic chip by the reduction of silver ions with hydrazine in ammonium hydroxide solution. Citrate ions are used as protecting agents. The synthesized nanoparticles are characterized by UV-VIS spectroscopy, SEM and TEM imaging. The SERS performance of the in situ synthesized nanoparticles is tested by using adenine as a test analyte right after the colloid synthesis...
November 23, 2016: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27779114/novel-one-pot-and-facile-room-temperature-synthesis-of-gold-nanodots-and-application-as-highly-sensitive-and-selective-probes-for-cyanide-detection
#4
Nagamalai Vasimalai, Maria T Fernandez-Arguelles
Highly fluorescent gold nanodots have been synthesized through a novel rapid, facile and one-pot room temperature route using trithiocyanuric acid as mild reducing agent and surface ligand. The proposed synthesis overcomes limitations of other synthetic routes in terms of cost, time, complexity and environmental risks, and gives rise to highly fluorescent gold nanodots within 10 min at room temperature, with a maximum emission wavelength at 623 nm and a large Stokes shift (213 nm). Moreover, the synthesized gold nanodots showed a large emission QY (9...
November 25, 2016: Nanotechnology
https://www.readbyqxmd.com/read/27670267/the-clock-trial-a-double-blinded-randomized-controlled-trial-trisodium-citrate-30-and-minocycline-3-mg-ml-plus-edta-30-mg-ml-are-effective-and-safe-for-catheter-patency-maintenance-among-ckd-5d-patients-on-hemodialysis
#5
Marcus V S João Luiz, Cristoforo Scavone, Carmen Tzanno
Introduction Poor blood flow rate (PF) is highly prevalent among CKD 5D patients with long-term central venous catheters. Heparin catheter lock solutions are commonly used to maintain catheter patency, however the incidence of PF remains high. The purpose of the CLOCK Trial was to evaluate two catheter lock solutions on reduction of PF incidence. Methods Seventy-five CKD 5D patients on high-efficiency hemodialysis at the Integrated Centre of Nephrology (Guarulhos, Brazil) were randomized 1:1:1 to receive a lock solution combining minocycline 3 mg/mL with the anticoagulant/chelation agent EDTA 30 mg/mL (M-EDTA) or heparin 1000 IU/mL (H) or trisodium citrate 30% (TSC) vs...
September 26, 2016: Hemodialysis International
https://www.readbyqxmd.com/read/27593160/isoexergonic-conformations-of-surface-bound-citrate-regulated-bioinspired-apatite-nanocrystal-growth
#6
Ziqiu Wang, Zhijun Xu, Weilong Zhao, Wei Chen, Toshikazu Miyoshi, Nita Sahai
The superior biomechanical properties of bone and dentin are dictated, in part, by the unique plate-like morphology of hydroxyapatite (HAP) nanocrysals within a hierarchically assembled collagen matrix. Understanding the mechanism of crystal growth and thus morphology is important to the rational design of bioinspired apatite nanocrystals for orthopedic and dental applications. Citrate has long been proposed to modulate apatite crystal growth, but major questions exist regarding the HAP-bound citrate conformations and the identities of the interacting functional groups and HAP surface sites...
October 5, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27500213/cyclic-helix-b-peptide-in-preservation-solution-and-autologous-blood-perfusate-ameliorates-ischemia-reperfusion-injury-in-isolated-porcine-kidneys
#7
Cheng Yang, Sarah A Hosgood, Patel Meeta, Yaqiu Long, Tongyu Zhu, Michael L Nicholson, Bin Yang
UNLABELLED: There is a critical need to better preserve isolated organs before transplantation. We developed a novel nonerythropoiesis cyclic helix B peptide (CHBP) derived from erythropoietin, which has potent tissue protection and prolonged serum stability. The renoprotection and potential mechanism of CHBP were evaluated in a kidney preservation model. MATERIALS AND METHODS: Porcine kidneys (n = 5) subjected to 20-minute warm ischemia were retrieved and flushed with hyperosmolar citrate to mimic deceased donation...
March 2015: Transplantation Direct
https://www.readbyqxmd.com/read/27222612/formulation-development-process-optimization-and-in-vitro-characterization-of-spray-dried-lansoprazole-enteric-microparticles
#8
Chintan Vora, Riddhish Patadia, Karan Mittal, Rajashree Mashru
This research focuses on the development of enteric microparticles of lansoprazole in a single step by employing the spray drying technique and studies the effects of variegated formulation/process variables on entrapment efficiency and in vitro gastric resistance. Preliminary trials were undertaken to optimize the type of Eudragit and its various levels. Further trials included the incorporation of plasticizer triethyl citrate and combinations of other polymers with Eudragit S 100. Finally, various process parameters were varied to investigate their effects on microparticle properties...
April 2016: Scientia Pharmaceutica
https://www.readbyqxmd.com/read/26971068/gene-delivery-using-calcium-phosphate-nanoparticles-optimization-of-the-transfection-process-and-the-effects-of-citrate-and-poly-l-lysine-as-additives
#9
Mohammed A Khan, Victoria M Wu, Shreya Ghosh, Vuk Uskoković
Despite the long history of nanoparticulate calcium phosphate (CaP) as a non-viral transfection agent, there has been limited success in attempts to optimize its properties for transfection comparable in efficiency to that of viral vectors. Here we focus on the optimization of: (a) CaP nanoparticle precipitation conditions, predominantly supersaturation and Ca/P molar ratios; (b) transfection conditions, mainly the concentrations of the carrier and plasmid DNA; (c) the presence of surface additives, including citrate anion and cationic poly(l-lysine) (PLL)...
June 1, 2016: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/26969510/a-method-to-preserve-low-parasitaemia-plasmodium-infected-avian-blood-for-host-and-vector-infectivity-assays
#10
Jenny S Carlson, Federico Giannitti, Gediminas Valkiūnas, Lisa A Tell, Joy Snipes, Stan Wright, Anthony J Cornel
BACKGROUND: Avian malaria vector competence studies are needed to understand more succinctly complex avian parasite-vector-relations. The lack of vector competence trials may be attributed to the difficulty of obtaining gametocytes for the majority of Plasmodium species and lineages. To conduct avian malaria infectivity assays for those Plasmodium spp. and lineages that are refractory to in vitro cultivation, it is necessary to obtain and preserve for short periods sufficient viable merozoites to infect naïve donor birds to be used as gametocyte donors to infect mosquitoes...
2016: Malaria Journal
https://www.readbyqxmd.com/read/26966916/three-dimensional-alginate-bead-culture-of-human-pituitary-adenoma-cells
#11
Dulce Avila-Rodríguez, Karina Paisano-Cerón, Irene Valdovinos-Ramírez, Carmen Solano-Agama, Alma Ortiz-Plata, María E Mendoza-Garrido
A three-dimensional culture method is described in which primary pituitary adenoma cells are grown in alginate beads. Alginate is a polymer derived from brown sea algae. Briefly, the tumor tissue is cut into small pieces and submitted to an enzymatic digestion with collagenase and trypsin. Next, a cell suspension is obtained. The tumor cell suspension is mixed with 1.2% sodium alginate and dropped into a CaCl2 solution, and the alginate/cell suspension is gelled on contact with the CaCl2 to form spherical beads...
2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/26963496/electrophoretic-separation-of-single-particles-using-nanoscale-thermoplastic-columns
#12
Kumuditha M Weerakoon-Ratnayake, Franklin I Uba, Nyoté J Oliver-Calixte, Steven A Soper
Phenomena associated with microscale electrophoresis separations cannot, in many cases, be applied to the nanoscale. Thus, understanding the electrophoretic characteristics associated with the nanoscale will help formulate relevant strategies that can optimize the performance of separations carried out on columns with at least one dimension below 150 nm. Electric double layer (EDL) overlap, diffusion, and adsorption/desorption properties and/or dielectrophoretic effects giving rise to stick/slip motion are some of the processes that can play a role in determining the efficiency of nanoscale electrophoretic separations...
April 5, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/26895948/analysis-of-silver-and-gold-nanoparticles-in-environmental-water-using-single-particle-inductively-coupled-plasma-mass-spectrometry
#13
Yuan Yang, Chen-Lu Long, Hai-Pu Li, Qiang Wang, Zhao-Guang Yang
The production and use of engineering nanomaterials (ENMs) leads to the release of manufactured or engineered nanoparticles into environment. The quantification and characterization of ENMs are crucial for the assessment of their environmental fate, transport behavior and health risks to humans. To analyze the size distribution and particle number concentration of AgNPs and AuNPs in environmental water and track their stability at low number concentration, a systematic study on SP-ICPMS was presented. The Poisson statistics was used to discuss the effect of dwell time and particle number concentration theoretically on the detection of NPs in solution by SP-ICPMS...
September 1, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/26855862/immunohistochemistry-for-the-detection-of-neural-and-inflammatory-cells-in-equine-brain-tissue
#14
Gretchen H Delcambre, Junjie Liu, Jenna M Herrington, Kelsey Vallario, Maureen T Long
Phenotypic characterization of cellular responses in equine infectious encephalitides has had limited description of both peripheral and resident cell populations in central nervous system (CNS) tissues due to limited species-specific reagents that react with formalin-fixed, paraffin embedded tissue (FFPE). This study identified a set of antibodies for investigating the immunopathology of infectious CNS diseases in horses. Multiple commercially available staining reagents and antibodies derived from antigens of various species for manual immunohistochemistry (IHC) were screened...
2016: PeerJ
https://www.readbyqxmd.com/read/26656229/comparisons-of-aluminum-and-silica-elution-from-various-glass-vials
#15
Toru Ogawa, Makoto Miyajima, Norihiro Nishimoto, Hidemi Minami, Katsuhide Terada
To understand the risk of particle formation in glass vials, we investigated the correlation between vial surface condition and alminum (Al) or silicon (Si) elution using various suppliers' vials with or without surface treatment. The elution of Si, which can also be an indicator of Al elution, consists of two phases; the first phase is influenced by roughness of the glass surface at the time of filling, and the second phase is dependent on the fundamental elution rate from the glass tube. When vials were filled with citrate buffer at pH 7, vials with varied surface conditions showed the most obvious differences in Al and Si elution...
2016: Chemical & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/26353696/quantitative-replacement-of-citrate-by-phosphane-on-silver-nanoparticle-surfaces-monitored-by-surface-enhanced-raman-spectroscopy-sers
#16
Stefan Grass, Jörg Diendorf, Julia S Gebauer, Matthias Epple, Lennart Treuel
Chemical approaches to metal NP synthesis commonly use capping agents to achieve a desired NP size and shape. Frequently, such NPs require chemically different surface ligands after synthesis to generate desired NP properties (e.g., charge or hydrophilicity) and to increase their long term colloidal stability. Here, we prepared SERS active citrate-stabilized silver NPs (d = 38±4 nm), purified them from remaining reactants by ultracentrifugation and redispersion, and immersed them into solutions containing different concentrations of Tris(sodium-m-sulfonatophenyl)phosphine (TPPTS), which is often used in such ligand replacement approaches to increase colloidal stability...
February 2015: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/26302922/an-approach-for-manganese-biomonitoring-using-a-manganese-carrier-switch-in-serum-from-transferrin-to-citrate-at-slightly-elevated-manganese-concentration
#17
B Michalke, L Aslanoglou, M Ochsenkühn-Petropoulou, B Bergström, A Berthele, M Vinceti, M Lucio, G Lidén
After high-dose-short-term exposure (usually from occupational exposure) and even more under low-dose long term exposure (mainly environmental) manganese (Mn) biomonitoring is still problematic since these exposure scenarios are not necessarily reflected by a significant increase of total Mn in blood or serum. Usually, Mn concentrations of exposed and unexposed persons overlap and individual differentiation is often not possible. In this paper Mn speciation on a large sample size (n=180) was used in order to be able to differentiate between highly Mn-exposed or low or unexposed individuals at low total Mn concentration in serum (Mn(S))...
October 2015: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/26282899/irrigation-with-n-n-dichloro-2-2-dimethyltaurine-nvc-422-in-a-citrate-buffer-maintains-urinary-catheter-patency-in-vitro-and-prevents-encrustation-by-proteus-mirabilis
#18
Suriani Abdul Rani, Chris Celeri, Ron Najafi, Keith Bley, Dmitri Debabov
Long-term use of indwelling urinary catheters can lead to urinary tract infections and loss of catheter patency due to encrustation and blockage. Encrustation of urinary catheters is due to formation of crystalline biofilms by urease-producing microorganisms such as Proteus mirabilis. An in vitro catheter biofilm model (CBM) was used to evaluate current methods for maintaining urinary catheter patency. We compared antimicrobial-coated urinary Foley catheters, with both available catheter irrigation solutions and investigational solutions containing NVC-422 (N,N-dichloro-2,2-dimethyltaurine; a novel broad-spectrum antimicrobial)...
June 2016: Urolithiasis
https://www.readbyqxmd.com/read/26069766/regional-citrate-anticoagulation-for-slow-continuous-ultrafiltration-risk-of-severe-metabolic-alkalosis
#19
Mourad M Alsabbagh, A Ahsan Ejaz, Daniel L Purich, Edward A Ross
BACKGROUND: Slow continuous ultrafiltration (SCUF) is a safe and efficient treatment for fluid overload in patients who are hemodynamically unstable, have low urine output, and are not in need of dialysis or hemofiltration for solute clearance. Sustained anticoagulation is required for these long treatments, thus posing clinically challenges for patients having contraindications to systemic anticoagulation with heparin. Regional citrate anticoagulation would be an alternative option; however, we believed that this would be problematic due to citrate kinetics that predicted the development of metabolic alkalosis...
June 2012: Clinical Kidney Journal
https://www.readbyqxmd.com/read/26067896/synthetic-wastewaters-treatment-by-electrocoagulation-to-remove-silver-nanoparticles-produced-by-different-routes
#20
M S Matias, S P Melegari, D S Vicentini, W G Matias, C Ricordel, D Hauchard
Nanoscience is a field that has stood out in recent years. The accurate long-term health and environmental risks associated with these emerging materials are unknown. Therefore, this work investigated how to eliminate silver nanoparticles (AgNPs) from synthetic effluents by electrocoagulation (EC) due to the widespread use of this type of nanoparticle (NP) in industry and its potential inhibition power over microorganisms responsible for biological treatment in effluent treatment plants. Synthesized AgNPs were studied via four different routes by chemical reduction in aqueous solutions to simulate the chemical variations of a hypothetical industrial effluent, and efficiency conditions of the EC treatment were determined...
August 15, 2015: Journal of Environmental Management
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