keyword
MENU ▼
Read by QxMD icon Read
search

THP-1 + inflammation

keyword
https://www.readbyqxmd.com/read/28545809/macrophage-specific-nanotechnology-driven-cd163-overexpression-in-human-macrophages-results-in-an-m2-phenotype-under-inflammatory-conditions
#1
Perla Abigail Alvarado-Vazquez, Laura Bernal, Candler A Paige, Rachel L Grosick, Carolina Moracho Vilrriales, David Wilson Ferreira, Cristina Ulecia-Morón, E Alfonso Romero-Sandoval
M1 macrophages release proinflammatory factors during inflammation. They transit to an M2 phenotype and release anti-inflammatory factors to resolve inflammation. An imbalance in the transition from M1 to M2 phenotype in macrophages contributes to the development of persistent inflammation. CD163, a member of the scavenger receptor cysteine-rich family, is an M2 macrophage marker. The functional role of CD163 during the resolution of inflammation is not completely known. We postulate that CD163 contributes to the transition from M1 to M2 phenotype in macrophages...
May 16, 2017: Immunobiology
https://www.readbyqxmd.com/read/28542410/gla-rich-protein-function-as-an-anti-inflammatory-agent-in-monocytes-macrophages-implications-for-calcification-related-chronic-inflammatory-diseases
#2
Carla S B Viegas, Rúben M Costa, Lúcia Santos, Paula A Videira, Zélia Silva, Nuna Araújo, Anjos L Macedo, António P Matos, Cees Vermeer, Dina C Simes
Calcification-related chronic inflammatory diseases are multifactorial pathological processes, involving a complex interplay between inflammation and calcification events in a positive feed-back loop driving disease progression. Gla-rich protein (GRP) is a vitamin K dependent protein (VKDP) shown to function as a calcification inhibitor in cardiovascular and articular tissues, and proposed as an anti-inflammatory agent in chondrocytes and synoviocytes, acting as a new crosstalk factor between these two interconnected events in osteoarthritis...
2017: PloS One
https://www.readbyqxmd.com/read/28528362/budesonide-fluticasone-propionate-and-azithromycin-do-not-modulate-the-membrane-vesicle-release-by-thp-1-macrophages-and-respiratory-pathogens-during-macrophage-infection
#3
Charlotte Volgers, Gert E Grauls, Pauline H M Hellebrand, Paul H M Savelkoul, Frank R M Stassen
Patients with more severe chronic obstructive pulmonary disease frequently experience exacerbations and it is estimated that up to 50% of these exacerbations are associated with bacterial infections. The mainstay treatment for these infection-related exacerbations constitutes the administration of glucocorticoids, alone or in combination with antibiotics. A recent line of evidence demonstrates that many hormones including the steroid beclomethasone can also directly affect bacterial growth, virulence, and antibiotic resistance...
May 20, 2017: Inflammopharmacology
https://www.readbyqxmd.com/read/28527850/complement-activation-by-pulp-capping-materials-plays-a-significant-role-in-both-inflammatory-and-pulp-stem-cells-recruitment
#4
Thomas Giraud, Pierre Rufas, Fanny Chmilewsky, Charlotte Rombouts, Jacques Dejou, Charlotte Jeanneau, Imad About
INTRODUCTION: The role of complement, especially through the C5a fragment, is well-known for the initiation of inflammation. Its involvement in regeneration has been shown more recently by the recruitment of mesenchymal stem cells. C5a can be produced locally by the pulp fibroblasts in response to injury or infection. This work aims to investigate the effect of different pulp capping biomaterials on complement activation and its possible influence on inflammatory and pulp stem cell recruitment...
May 17, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28522896/shear-stress-counteracts-endothelial-cx3cl1-induction-and-monocytic-cell-adhesion
#5
Aaron Babendreyer, Lisa Molls, Daniela Dreymueller, Stefan Uhlig, Andreas Ludwig
Flow conditions critically regulate endothelial cell functions in the vasculature. Reduced shear stress resulting from disturbed blood flow can drive the development of vascular inflammatory lesions. On endothelial cells, the transmembrane chemokine CX3CL1/fractalkine promotes vascular inflammation by functioning as a surface-expressed adhesion molecule and by becoming released as soluble chemoattractant for monocytic cells expressing the receptor CX3CR1. Here, we report that endothelial cells from human artery, vein, or microvasculature constitutively express CX3CL1 when cultured under static conditions...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/28522271/medroxyprogesterone-acetate-drives-m2-macrophage-differentiation-toward-a-phenotype-of-decidual-macrophage
#6
Yung-Chieh Tsai, Joseph T Tseng, Chia-Yih Wang, Mei-Tsz Su, Jyun-Yuan Huang, Pao-Lin Kuo
M1 macrophage differentiation plays a crucial role in enhanced inflammation during pregnancy, which may lead to pregnancy complications. Therefore, modulation of macrophage differentiation toward the M2 phenotype is desirable to ensure a successful pregnancy. Medroxyprogesterone acetate (MPA) is a potent progestin with an anti-inflammatory property, but its effect on macrophage differentiation is unknown. This study aimed to examine whether MPA can induce an M2 macrophage differentiation by using the human monocytes cell line THP-1 or primary monocytes...
May 15, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28512001/low-dose-inflammatory-potential-of-silica-particles-in-human-derived-thp-1-macrophage-cell-culture-studies-mechanism-and-effects-of-particle-size-and-iron
#7
Premshekharan Gayatri, Hongqiao Zhang, Kennedy Nguyen, Henry Jay Forman, Valerie Jean Leppert
Silica and iron are major constituents in ambient particulate matter, and iron is a common impurity in many engineered nanomaterials. The purpose of this work was to determine the pro-inflammatory and other biological effects and mechanism of particle size and iron presence under low dose, non-cytotoxic conditions that are likely to approximate actual exposure levels, in contrast with higher dose studies in which cytotoxicity occurs. Specifically, human-derived THP-1 macrophages were exposed to 1 μg/ml of pristine and iron-coated 50 nm and 2 μm engineered silica nanoparticles...
May 13, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28506804/chloroquine-enhances-the-antimycobacterial-activity-of-isoniazid-and-pyrazinamide-by-reversing-inflammation-induced-macrophage-efflux
#8
U Matt, P Selchow, M Dal Molin, S Strommer, O Sharif, K Schilcher, F Andreoni, A Stenzinger, A S Zinkernagel, M Zeitlinger, P Sander, J Nemeth
Mycobacterium tuberculosis (MTB) is notorious for persisting within host macrophages. Efflux pumps decrease intracellular drug levels, thus fostering persistence of MTB during therapy. Isoniazid (INH) and pyrazinamide (PZA) are substrates of the efflux pump breast cancer resistance protein-1 (BCRP-1), which is inhibited by chloroquine (CQ). In this study, BCRP-1 was found to be expressed on macrophages of human origin and on foamy giant cells at the site of MTB infection. In the current in vitro study, interferon-gamma (IFNγ) increased the expression of BCRP-1 in macrophages derived from the human monocytic leukaemia cell line THP-1...
May 12, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28494352/il-33-protects-murine-viral-fulminant-hepatitis-by-targeting-coagulation-hallmark-protein-fgl2-fibroleukin-expression
#9
Haijing Yu, Yang Liu, Jiaquan Huang, Hongwu Wang, Weiming Yan, Dong Xi, Guanxin Shen, Xiaoping Luo, Qin Ning
Fulminant hepatitis (FH) is characterized by rapid liver failure and high mortality. The pathogenesis of viral FH includes virus-induced immune activation, inflammation, and subsequent hepatic apoptosis and necrosis. However, the mechanisms that underlie FH progression are unclear. IL-33 is a member of the IL-1-related cytokines, considered to be an "alarmin" that participates in various diseases, but its precise role in the coagulation of FH is not very clear. In our study, we found that IL-33 is significantly elevated in mice infected with murine hepatitis virus strain 3 (MHV-3)...
May 8, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28493827/tlr2-activation-induces-antioxidant-defence-in-human-monocyte-macrophage-cell-line-models
#10
Iwona Karwaciak, Michal Gorzkiewicz, Grzegorz Bartosz, Lukasz Pulaski
When monocytes are recruited to inflammation/infection sites, extravasate and differentiate into macrophages, they encounter increasing levels of oxidative stress, both from exogenous and endogenous sources. In this study, we aimed to determine whether there are specific biochemical mechanisms responsible for an increase in oxidative stress resistance in differentiating macrophages. We performed experiments on in vitro cell line models of the monocyte-macrophage differentiation axis (less differentiated THP-1 cells and more differentiated Mono Mac 6 cells)...
April 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/28472719/exposure-to-porphyromonas-gingivalis-lps-during-macrophage-polarisation-leads-to-diminished-inflammatory-cytokine-production
#11
Louise A Belfield, Jon H Bennett, Wondwossen Abate, Simon K Jackson
OBJECTIVE: The objective of the present study was to determine the effects of concurrent LPS and cytokine priming, reflective of the in vivo milieu, on macrophage production of key periodontitis associated cytokines TNF, IL-1β and IL-6. DESIGN: THP-1 cells were pre-treated with combinations of Porphyromonas gingivalis and Escherichia coli lipopolysaccharide (LPS), concurrently with polarising cytokines IFNγ and IL-4, or PMA as a non-polarised control. Production of key periodontitis associated cytokines in response to subsequent LPS challenge were measured by enzyme - linked immunosorbent assay...
April 20, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28465217/antidepressants-induce-autophagy-dependent-nlrp3-inflammasome-inhibition-in-major-depressive-disorder
#12
Elísabet Alcocer-Gómez, Nieves Casas-Barquero, Matthew R Williams, Samuel L Romero-Guillena, Diego Cañadas-Lozano, Pedro Bullón, José Antonio Sánchez-Alcazar, José M Navarro-Pando, Mario D Cordero
Major Depressive Disorder (MDD, ICD-10: F-33) is a prevalent illness in which the pathogenic mechanism remains elusive. Recently an important role has been attributed to neuro-inflammation, and specifically the NLRP3-inflammasome complex, in the pathogenesis of MDD. This suggests a key role for immunomodulation as a key pathway in the treatment of this disorder. This study evaluates the involvement of nine common antidepressants in the NLRP3-inflammasome complex (fluoxetine, paroxetine, mianserin, mirtazapine, venlafaxine, desvenlafaxine, amitriptyline, imipramine and agomelatine), both in in vitro THP-1 cells stimulated by ATP, and in a stress-induced depressive animal or MDD patients...
May 2, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/28458470/intrinsic-properties-of-brown-and-white-adipocytes-have-differential-effects-on-macrophage-inflammatory-responses
#13
Louisa Dowal, Pooja Parameswaran, Sarah Phat, Syamala Akella, Ishita Deb Majumdar, Jyoti Ranjan, Chahan Shah, Saie Mogre, Kalyani Guntur, Khampaseuth Thapa, Stephane Gesta, Vivek K Vishnudas, Niven R Narain, Rangaprasad Sarangarajan
Obesity is marked by chronic, low-grade inflammation. Here, we examined whether intrinsic differences between white and brown adipocytes influence the inflammatory status of macrophages. White and brown adipocytes were characterized by transcriptional regulation of UCP-1, PGC1α, PGC1β, and CIDEA and their level of IL-6 secretion. The inflammatory profile of PMA-differentiated U937 and THP-1 macrophages, in resting state and after stimulation with LPS/IFN-gamma and IL-4, was assessed by measuring IL-6 secretion and transcriptional regulation of a panel of inflammatory genes after mono- or indirect coculture with white and brown adipocytes...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/28451522/ureaplasma-species-multiple-banded-antigen-mba-variation-is-associated-with-the-severity-of-inflammation-in-vivo-and-in-vitro-in-human-placentae
#14
Emma L Sweeney, Suhas G Kallapur, Simone Meawad, Tate Gisslen, Sally-Anne Stephenson, Alan H Jobe, Christine L Knox
Background: The multiple banded antigen (MBA), a surface-exposed lipoprotein, is a proposed virulence factor of Ureaplasma spp. We previously demonstrated that the number of Ureaplasma parvum MBA size variants in amniotic fluid was inversely proportional to the severity of chorioamnionitis in experimentally infected pregnant sheep. However, the effect of ureaplasma MBA size variation on inflammation in human pregnancies has not been reported. Methods: Ureaplasmas isolated from the chorioamnion of pregnant women from a previous study (n = 42) were speciated/serotyped and MBA size variation was demonstrated by PCR and western blot...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28444107/apelin-13-inhibits-lipoprotein-lipase-expression-via-the-apj-pkc%C3%AE-mir-361-5p-signaling-pathway-in-thp-1-macrophage-derived-foam-cells
#15
Xin Zhang, Qiong Ye, Duo Gong, Yuan Lv, Haipeng Cheng, Chong Huang, Lingyan Chen, Zhenwang Zhao, Liang Li, Xie Wei, Min Zhang, Xiaodan Xia, Xiaohua Yu, Xilong Zheng, Shuzhi Wang, Zongbao Wang, Chaoke Tang
Atherosclerotic lesions are characterized by the accumulation of abundant lipids and chronic inflammation. Previous researches have indicated that macrophage-derived lipoprotein lipase (LPL) promotes atherosclerosis progression by accelerating lipid accumulation and pro-inflammatory cytokine secretion. Although apelin-13 has been regarded as an atheroprotective factor, it remains unclear whether it can regulate the expression of LPL. The aim of this study was to explore the effects of apelin-13 on the expression of LPL and the underlying mechanism in THP-1 macrophage-derived foam cells...
April 21, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28441337/a-study-of-the-differential-effects-of-eicosapentaenoic-acid-epa-and-docosahexaenoic-acid-dha-on-gene-expression-profiles-of-stimulated-thp-1-macrophages
#16
Bénédicte Allam-Ndoul, Frédéric Guénard, Olivier Barbier, Marie-Claude Vohl
Background: An appropriate intake of omega-3 (n-3) fatty acids (FAs) such as eicosapentaenoic and docosahexaenoic acid (EPA/DHA) from marine sources is known to have anti-inflammatory effects. However, molecular mechanisms underlying their beneficial effects on health are not fully understood. The aim of the present study was to characterize gene expression profiles of THP-1 macrophages, incubated in either EPA or DHA and stimulated with lipopolysaccharide (LPS), a pro-inflammatory agent. Methods: THP-1 macrophages were incubated into 10, 50 and 75 µM of EPA or DHA for 24 h, and 100 nM of LPS was added to the culture media for 18 h...
April 25, 2017: Nutrients
https://www.readbyqxmd.com/read/28440395/microrna-mediated-inflammatory-responses-induced-by-cryptococcus-neoformans-are-dependent-on-the-nf-%C3%AE%C2%BAb-pathway-in-human-monocytes
#17
Hong Chen, Yi Jin, Huan Chen, Ningxin Liao, Yan Wang, Jianghan Chen
Cryptococcosis is a significant invasive fungal infection with noteworthy morbidity and mortality that is usually caused by either Cryptococcus neoformans (C. neoformans) or Cryptococcus gattii (C. gattii). Epidemiological studies have indicated that C. neoformans are more often reported in immunocompromised and immunocompetent patients. It has been well established that the cytokine profile of the host markedly affects the outcome of cryptococcal disease, and the negative regulators of microRNAs(miRs or miRNAs) are critically important for immunomodulation...
April 12, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28431555/reduction-of-pulmonary-toxicity-of-metal-oxide-nanoparticles-by-phosphonate-based-surface-passivation
#18
Xiaoming Cai, Anson Lee, Zhaoxia Ji, Cynthia Huang, Chong Hyun Chang, Xiang Wang, Yu-Pei Liao, Tian Xia, Ruibin Li
BACKGROUND: The wide application of engineered nanoparticles has induced increasing exposure to humans and environment, which led to substantial concerns on their biosafety. Some metal oxides (MOx) have shown severe toxicity in cells and animals, thus safe designs of MOx with reduced hazard potential are desired. Currently, there is a lack of a simple yet effective safe design approach for the toxic MOx. In this study, we determined the key physicochemical properties of MOx that lead to cytotoxicity and explored a safe design approach for toxic MOx by modifying their hazard properties...
April 21, 2017: Particle and Fibre Toxicology
https://www.readbyqxmd.com/read/28429235/regulation-of-human-brain-microvascular-endothelial-cell-adhesion-and-barrier-functions-by-memantine
#19
Fei Wang, Zhirong Zou, Yi Gong, Dong Yuan, Xun Chen, Tao Sun
Vascular risk factors have been linked to cognitive decline and dementia in the elderly. Microvascular inflammation, especially of the endothelium, may contribute to the progression of neurodegenerative events in Alzheimer's disease (AD). Memantine, an uncompetitive N-methyl-D-aspartate (NMDA) receptor antagonist, is a licensed drug used for the treatment of moderate to severe AD. However, little information is available regarding its anti-inflammatory effects on the endothelium. In this study, we investigated the effects of memantine on human brain microvascular endothelial dysfunction induced by the pro-inflammatory cytokine tumor necrosis factor-α (TNF-α)...
April 20, 2017: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/28421819/il-4-induces-the-il17rb-gene-transcription-in-monocytic-cells-with-coordinate-autocrine-il-25-signaling
#20
Nathaniel M Weathington, Shreya S Kanth, Qiaoke Gong, James Londino, Akihiko Hoji, Mauricio Rojas, John Trudeau, Sally Wenzel, Rama K Mallampalli
IL-25 and IL-4 signaling in the setting of infection or allergic responses can drive Type 2 inflammation. IL-25 requires the IL-17 receptor B (IL-17Rb) to mediate signaling through nuclear factor kappa B transcriptional activation. Despite the known coexistence of these two cytokines in the Type 2 inflammatory environment, collaborative signaling between the IL-4 and IL-25 axes is poorly explored. Here we demonstrate IL-4 induction of both IL-25 and IL-17Rb protein in human lung tissue culture, primary alveolar macrophages, and the THP-1 monocytic cell line...
April 19, 2017: American Journal of Respiratory Cell and Molecular Biology
keyword
keyword
119090
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"