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https://www.readbyqxmd.com/read/29166412/mir-195-inhibits-macrophages-pro-inflammatory-profile-and-impacts-the-crosstalk-with-smooth-muscle-cells
#1
Joao Paulo Bras, Andreia Machado Silva, George A Calin, Mario Adolfo Barbosa, Susana Gomes Santos, Maria Ines Almeida
Macrophages are a main component of atherosclerotic plaques. Recent studies suggest that pro-inflammatory M1 macrophages are pro-atherogenic while M2 macrophages promote plaque stability. Moreover, toll-like receptor signalling pathways are implicated in atherosclerotic plaque formation, evolution and regression. We propose microRNAs as key regulators of these processes. In this context, our goal is to promote inflammation resolution using miR-195 to reduce M1-like macrophage polarization and to evaluate the molecular mechanisms underlying such effect, as well as to explore the functional consequences for smooth muscle cell recruitment...
2017: PloS One
https://www.readbyqxmd.com/read/29149784/anti-inflammatory-property-of-%C3%AE-d-mannuronic-acid-m2000-on-expression-and-activity-of-matrix-metalloproteinase-2-and-9-through-cd147-molecule-in-phorbol-myristate-acetate-differentiated-thp-1-cells
#2
Mohadese M Farahani, Elahe Motevaseli, Faezeh Maghsood, Maryam Heidari-Kharaji, Abbas Mirshafiey
The aim of this study was to evaluate the effects of M2000, a novel non-steroidal anti-inflammatory drug (NSAID) with immunosuppressive property and without gastro-nephrotoxicitic effects on matrix metalloproteinases (MMP)-2 and (MMP)-9 in phorbol myristate acetate (PMA)-differentiated THP-1 cells. Gene expression and activity of MMP-2 and MMP-9 are inhibited respectively by the tissue inhibitor of matrix metalloproteinase (TIMP)-2 and (TIMP)-1 and are induced by extracellular matrix metalloproteinase inducer (CD147/EMMPRIN)...
October 2017: Iranian Journal of Allergy, Asthma, and Immunology
https://www.readbyqxmd.com/read/29145789/helicobacter-pylori-controls-nlrp3-expression-by-regulating-hsa-mir-223-3p-and-il-10-in-cultured-and-primary-human-immune-cells
#3
Suneesh Kumar Pachathundikandi, Steffen Backert
Inflammasome-mediated production of mature IL-1β and IL-18 cytokines represents an important innate immune response against infecting pathogens. Helicobacter pylori, one of the most successful and persistent human pathogens, induces severe inflammation leading to gastritis and more serious gastric diseases. H. pylori modulates different immune responses for its survival and inflammasome signaling is manipulated by the cag pathogenicity island ( cagPAI), urease and VacA cytotoxin. Here we report that H. pylori regulates NLRP3 expression, an inflammasome forming regulator, in infected THP-1 monocytes...
January 1, 2017: Innate Immunity
https://www.readbyqxmd.com/read/29137283/c1q-tumor-necrosis-factor-related-protein-3-protects-macrophages-against-lps-induced-lipid-accumulation-inflammation-and-phenotype-transition-via-ppar%C3%AE-and-tlr4-mediated-pathways
#4
Jiale Lin, Qi Liu, Hui Zhang, Xingtao Huang, Ruoxi Zhang, Shuyuan Chen, Xuedong Wang, Bo Yu, Jingbo Hou
Macrophage inflammation and foam cell formation are critical events during the initiation and development of atherosclerosis (AS). C1q/tumor necrosis factor-related protein-3 (CTRP3) is a novel adipokine with anti-inflammatory and cardioprotection properties; however, little is known regarding the influence of CTRP3 on AS. As macrophages play a key role in AS, this study investigated the effects of CTRP3 on macrophage lipid metabolism, inflammatory reactions, and phenotype transition, as well as underlying mechanisms, to reveal the relationship between CTRP3 and AS...
October 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29134442/endothelial-tnf-%C3%AE-induction-by-hsp60-secreted-from-thp-1-monocytes-exposed-to-hyperglycaemic-conditions
#5
Ryan Dennis Martinus, Julie Goldsbury
A non-resolving inflammation of the endothelium is recognised to be an important process leading to atherosclerosis. In diabetes, this process is thought to account for a significant number of cardiovascular disease-associated death and disability. However, the molecular mechanisms by which diabetes contributes to endothelial inflammation remain to be established. Whilst there is some evidence linking hyperglycaemia-induced reactive oxygen species (ROS) formation by the mitochondrial electron-transport chain to oxidative stress, cellular injury and apoptosis in the endothelium, a clear link to endothelium inflammation has not yet been established...
November 13, 2017: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/29132302/immunomodulatory-role-for-membrane-vesicles-released-by-thp-1-macrophages-and-respiratory-pathogens-during-macrophage-infection
#6
Charlotte Volgers, Birke J Benedikter, Gert E Grauls, Paul H M Savelkoul, Frank R M Stassen
BACKGROUND: During infection, inflammation is partially driven by the release of mediators which facilitate intercellular communication. Amongst these mediators are small membrane vesicles (MVs) that can be released by both host cells and Gram-negative and -positive bacteria. Bacterial membrane vesicles are known to exert immuno-modulatory and -stimulatory actions. Moreover, it has been proposed that host cell-derived vesicles, released during infection, also have immunostimulatory properties...
November 13, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/29129670/microalgae-derived-oxylipins-decrease-inflammatory-mediators-by-regulating-the-subcellular-location-of-nf%C3%AE%C2%BAb-and-ppar-%C3%AE
#7
Javier Ávila-Román, Elena Talero, Carolina de Los Reyes, Sofía García-Mauriño, Virginia Motilva
Oxylipins (OXLs) are bioactive molecules generated by the oxidation of fatty acids that promote the resolution of acute inflammation and prevent chronic inflammatory processes through molecular mechanisms that are not well known. We have previously reported the anti-inflammatory activity of microalgae-derived OXLs and OXL-containing biomass in two inflammatory bowel disease (IBD) models: 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute colitis and TNBS-induced recurrent colitis. In this study, we examined the in vitro anti-inflammatory mechanism of action of the most abundant OXLs isolated from Chlamydomonas debaryana (13S-HOTE and 13S-HODE) and Nannochloropsis gaditana (15S-HEPE)...
November 9, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/29122211/citrate-dialysate-does-not-induce-oxidative-stress-or-inflammation-in-vitro-as-compared-to-acetate-dialysate
#8
Rafael Pérez-García, Rafael Ramírez Chamond, Patricia de Sequera Ortiz, Marta Albalate, Marta Puerta Carretero, Mayra Ortega, M Caridad Ruiz Caro, Roberto Alcazar Arroyo
Increased acetataemia during haemodialysis sessions has been associated with a number of abnormalities, including increased oxidative stress, pro-inflammatory cytokines and nitric oxide synthesis. However, citric acid may play an alternative role to acetate as a dialysate stabiliser given that the effect of citrate on complement and leukocyte activation is different to that of acetate. The purpose of this study was to compare the inflammatory effect in immunocompetent blood cells of acetate dialysate and citrate dialysate...
November 2017: Nefrología: Publicación Oficial de la Sociedad Española Nefrologia
https://www.readbyqxmd.com/read/29109173/the-homolog-of-the-gene-bsta-of-the-btp1-phage-from-salmonella-typhimurium-st313-is-an-antivirulence-gene-in-s-dublin
#9
Ana Herrero-Fresno, Irene Cartas Espinel, Malene Roed Spiegelhauer, Priscila Regina Guerra, Karsten Wiber Andersen, John Elmerdahl Olsen
In a previous study, a novel virulence gene, bstA, identified in S Typhimurium ST313, was found to be conserved in all published S Dublin genomes. In order to analyze the role of this gene in host-pathogen interaction in S. Dublin, mutants where this gene was deleted (SDΔbstA) and further genetically complemented (SD3246-C) were constructed and tested in in vitro and in vivo infection models as well as during growth competition assays in M9, LB and cattle blood. In contrast to the results obtained for a strain of S Typhimrium ST313, the lack of bstA was found to be associated with increased virulence in S...
November 6, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/29106735/human-periodontal-ligament-fibroblasts-synthesize-c-reactive-protein-and-th-related-cytokines-in-response-to-interleukin-il-6-trans-signaling
#10
A Hernández-Caldera, R Vernal, R Paredes, P Veloso-Matta, J Astorga, M Hernández
AIM: To characterize the potential of human periodontal ligament fibroblasts (HPLF) to synthesize CRP and Th-related cytokines in response to IL-6 in periodontal health and apical inflammation. METHODOLOGY: Primary HPLF stimulated with IL-6, soluble(s) IL-6 receptor (R) and controls were assayed for CRP, Th1, Th2, Th17 and Treg-related cytokines by quantitative real time PCR and ELISA, respectively. IL-6R mRNA expression and its soluble protein levels were screened in HPLF cultures, and ex vivo samples of healthy periodontal ligaments (n=5) and apical lesions (n=13)...
November 6, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/29097054/age-albumin-enhances-abca1-degradation-by-ubiquitin-proteasome-and-lysosomal-pathways-in-macrophages
#11
Rodrigo Tallada Iborra, Adriana Machado-Lima, Ligia Shimabukuro Okuda, Paula Ramos Pinto, Edna Regina Nakandakare, Ubiratan Fabres Machado, Maria Lucia Correa-Giannella, Russell Pickford, Tom Woods, Margaret A Brimble, Kerry-Anne Rye, Rui Lu, Shinji Yokoyama, Marisa Passarelli
BACKGROUND AND AIMS: Advanced glycation end products (AGEs) induce cellular oxidative/endoplasmic reticulum stress and inflammation. We investigated its underlying mechanisms for atherogenesis focusing on regulation of ABCA1 protein decay in macrophages. METHODS: The ABCA1 decay rate was evaluated in macrophages after treatment with LXR agonist and by incubation with control (C) or AGE-albumin concomitant or not with cycloheximide, MG-132, ammonium chloride and calpain inhibitors were utilized to inhibit, respectively, proteasome, lysosome and ABCA1 proteolysis at cell surface...
September 23, 2017: Journal of Diabetes and its Complications
https://www.readbyqxmd.com/read/29096169/lunasin-abrogates-monocytes-to-endothelial-cells
#12
Yongjun Zhu, Hui Li, Xuezhen Wang
The adherence of monocytes to endothelial cells plays a causal role in the early development of atherosclerosis and is driven by several inflammatory stimuli, which includes oxidized low-density lipoprotein (ox-LDL). Lunasin, a natural peptide identified in soybean seeds, soy-derived food products, other grains and herbal plants, has been found to exert numerous biological activities, including anti-inflammatory and antioxidant properties. However, little is known regarding the mechanism of action of lunasin in ox-LDL-induced endothelial inflammation...
October 30, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/29094619/acute-in-vitro-and-in-vivo-toxicity-of-a-commercial-grade-boron-nitride-nanotube-mixture
#13
Vamsi K Kodali, Jenny R Roberts, Mohammad Shoeb, Michael G Wolfarth, Lindsey Bishop, Tracy Eye, Mark Barger, Katherine A Roach, Sherri Friend, Diane Schwegler-Berry, Bean T Chen, Aleksandr Stefaniak, Kevin C Jordan, Roy R Whitney, Dale W Porter, Aaron D Erdely
Boron nitride nanotubes (BNNTs) are an emerging engineered nanomaterial attracting significant attention due to superior electrical, chemical and thermal properties. Currently, the toxicity profile of this material is largely unknown. Commercial grade BNNTs are composed of a mixture (BNNT-M) of ∼50-60% BNNTs, and ∼40-50% impurities of boron and hexagonal boron nitride. We performed acute in vitro and in vivo studies with commercial grade BNNT-M, dispersed by sonication in vehicle, in comparison to the extensively studied multiwalled carbon nanotube-7 (MWCNT-7)...
October 2017: Nanotoxicology
https://www.readbyqxmd.com/read/29090713/pepsin-pancreatin-hydrolysis-reduced-the-ability-of-lunasin-enriched-material-to-inhibit-activation-of-the-inflammasomes-in-thp-1-human-macrophages
#14
Samuel James Price, Philipus Pangloli, Vermont P Dia
Inflammation caused by the NLRP3 inflammasome has been linked to many diseases. Lunasin is a bioactive peptide from soybeans with reported anti-inflammatory properties. The objective of this work was to determine the effect of pepsin-pancreatin hydrolysis (PPH) on the ability of lunasin-enriched preparation (LEP) to inhibit inflammasome activation in differentiated THP-1 human macrophages. THP-1 macrophages were treated with different concentrations of LEP (0.0625 to 0.25 mg mL(-1)), primed with 1 μg mL(-1) lipopolysaccharide for 6 h and activated by 5 mM adenosine triphosphate for 1 h...
November 1, 2017: Food & Function
https://www.readbyqxmd.com/read/29083095/-shi-han-quan-natural-soda-water-prevents-development-of%C3%A2-gout
#15
Qingsong Zhao, Lipei Wang, Xiongbin Wu, Tingting Liu, Jinmei Xu, Ming Li, Bo Li, Min Guo, Zhifeng Cheng
PURPOSE: This study aimed to explore the regulatory mechanism of the natural soda Shi Han Quan (SHQ) in the development of gout. METHODS: Human umbilical vein endothelial cells (HUVECs) were stimulated with monosodium urate (MSU) for 24 h to induce acute gouty inflammation in vitro. HUVECs were divided into four groups: ddH2 O group, ddH2 O + MSU group, 1/2 ddH2 O +1/2 SHQ + MSU group, and SHQ + MSU group. The effects of SHQ on cell viability, concentration and expression of intercellular adhesion molecule-1 (ICAM-1), and MSU-induced release of interleukin (IL)-1β and IL-6 were investigated...
October 30, 2017: International Journal of Rheumatic Diseases
https://www.readbyqxmd.com/read/29080328/clinical-and-functional-significance-of-steap4-splice-variant-in-cd14-monocytes-in-patients-with-rheumatoid-arthritis
#16
Hiroshi Ebe, Isao Matsumoto, Hoshimi Kawaguchi, Izumi Kurata, Yuki Tanaka, Asuka Inoue, Yuya Kondo, Hiroto Tsuboi, Takayuki Sumida
Tumor necrosis factor alpha (TNFα)-induced adipose-related protein (TIARP) is a negative regulator of inflammation in arthritis model mice. In humans, six transmembrane epithelial antigen of prostate 4 (STEAP4) (human counterpart of TIARP) is also expressed in CD14(+) monocytes from patients with rheumatoid arthritis (RA). Recently, highly levels of exon3-spliced variant STEAP4 (v-STEAP4) expression have been observed in porcine lung. The aim of this study is to elucidate the expression and functional role of v-STEAP4, comparing it with that of STEAP4, in the pathogenesis of arthritis...
October 28, 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/29078913/naloxone-inhibits-nod-like-receptor-protein-3-inflammasome
#17
Han-Yu Lin, Ya-Ying Chang, Ming-Chang Kao, Chun-Jen Huang
BACKGROUND: Naloxone, an opioid receptor antagonist, possesses potent anti-inflammation effects. We previously confirmed the effects of naloxone on inhibiting upregulation of inflammatory cytokine interleukin-1β (IL-1β). Production of mature form IL-1β is mediated by the nod-like receptor protein 3 (NLRP3) inflammasome, a multiprotein complex composed of NLRP3, and the adaptor protein apoptosis-associated speck-like protein contains a caspase recruitment domain (ASC). We elucidated whether naloxone could inhibit the activation of NLRP3 inflammasome...
November 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/29078765/strain-dependent-interactions-of-streptococcus-gallolyticus-subsp-gallolyticus-with-human-blood-cells
#18
Imke Grimm, Melanie Weinstock, Ingvild Birschmann, Jens Dreier, Cornelius Knabbe, Tanja Vollmer
BACKGROUND: Streptococcus gallolyticus subsp. gallolyticus (S. gallolyticus) is the causative pathogen in up to 20% of streptococcal-induced infective endocarditis (IE) cases. However, the underlying mechanisms of pathogenesis in S. gallolyticus have not yet been solved. Pathogens causing IE need to employ virulent strategies to initiate and establish infections, such as escape the bloodstream, invade the host-cell, and persist intracellularly. In this study, we examined the induction of inflammation by different S...
October 27, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/29067554/extracellular-hsp70-induces-inflammation-and-modulates-lps-lta-stimulated-inflammatory-response-in-thp-1-cells
#19
Andrea Hulina, Marija Grdić Rajković, Daniela Jakšić Despot, Dubravko Jelić, Ana Dojder, Ivana Čepelak, Lada Rumora
Extracellular Hsp70 (eHsp70) can act as damage-associated molecular pattern (DAMP) via Toll-like receptors TLR2 and TLR4, and stimulate immune and inflammatory responses leading to sterile inflammation and propagation of already existing inflammation. It was found elevated in the blood of patients with chronic obstructive pulmonary disease (COPD), who might suffer occasional bacterial colonizations and infections. We used a monocytic THP-1 cell line as a cellular model of systemic compartment of COPD to assess inflammatory effects of eHsp70 when present alone or together with bacterial products lypopolysaccharide (LPS) and lypoteichoic acid (LTA)...
October 24, 2017: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/29062588/tlr4-ligands-selectively-synergize-to-induce-expression-of-il-8
#20
Lauren M Valenty, Christine M Longo, Carol Horzempa, Anthony Ambesi, Paula J McKeown-Longo
Objective: Dysfunctional remodeling of the extracellular matrix contributes to the formation of TLR-dependent feed forward loops that drive chronic inflammation. We have previously shown that two Type III domains of Fibronectin, FnEDA and FnIII-1c, cooperate to induce the synergistic release of interleukin 8 (IL-8) from dermal fibroblasts. We now identify steps in the TLR4 pathway where synergy can be demonstrated as well as additional kinases functioning in fibronectin activation of TLR4 signaling. We also evaluate the ligand and cell-type specificity of this synergistic response...
October 1, 2017: Advances in Wound Care
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