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https://www.readbyqxmd.com/read/28916288/anti-apoptotic-and-moderate-anti-inflammatory-effects-of-berberine-in-sulfur-mustard-exposed-keratinocytes
#1
Simon Lang, Tanja Popp, Christian Silvester Kriegs, Annette Schmidt, Frank Balszuweit, Georg Menacher, Kai Kehe, Horst Thiermann, Thomas Gudermann, Dirk Steinritz
Skin affections after sulfur mustard (SM) exposure include erythema, blister formation and severe inflammation. An antidote or specific therapy does not exist. Anti-inflammatory compounds as well as substances counteracting SM-induced cell death are under investigation. In this study, we investigated the benzylisoquinoline alkaloide berberine (BER), a metabolite in plants like berberis vulgaris, which is used as herbal pharmaceutical in Asian countries, against SM toxicity using a well-established in vitro approach...
September 12, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28916287/effects-of-anti-inflammatory-compounds-on-sulfur-mustard-injured-cells-recommendations-and-caveats-suggested-by-in-vitro-cell-culture-models
#2
Georg Menacher, Dirk Steinritz, Annette Schmidt, Tanja Popp, Franz Worek, Thomas Gudermann, Horst Thiermann, Frank Balszuweit
Sulfur mustard (SM) is a vesicant agent who had its first military use 100 years ago, in Ypres. Since then it has been used in several conflicts like the Iran-Iraq war in the 1980s. The use of SM in Syria 2015 indicated the still existing threat. Despite decades of research no causal antidote against SM intoxication is available, so far. A SM intoxication is accompanied by necrosis, apoptosis and inflammation. To counteract the SM-induced inflammation, glucocorticoids and non-steroidal anti-inflammatory compounds (NSAIDs) are recommended...
September 12, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28903338/tlr2-activation-induces-antioxidant-defence-in-human-monocyte-macrophage-cell-line-models
#3
Iwona Karwaciak, Michal Gorzkiewicz, Grzegorz Bartosz, Lukasz Pulaski
When monocytes are recruited to inflammation/infection sites, extravasate and differentiate into macrophages, they encounter increasing levels of oxidative stress, both from exogenous and endogenous sources. In this study, we aimed to determine whether there are specific biochemical mechanisms responsible for an increase in oxidative stress resistance in differentiating macrophages. We performed experiments on in vitro cell line models of the monocyte-macrophage differentiation axis (less differentiated THP-1 cells and more differentiated Mono Mac 6 cells)...
August 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28890654/chondroitin-sulfate-inhibits-monocyte-chemoattractant-protein-1-release-from-3t3-l1-adipocytes-a-new-treatment-opportunity-for-obesity-related-inflammation
#4
Thomas V Stabler, Eulàlia Montell, Josep Vergés, Janet L Huebner, Virginia Byers Kraus
Monocyte chemoattractant protein-1 (MCP-1) overproduction from inflamed adipose tissue is a major contributor to obesity-related metabolic syndromes. 3T3-L1 embryonic fibroblasts were cultured and differentiated into adipocytes using an established protocol. Adipocytes were treated with lipopolysaccharide (LPS) to induce inflammation and thus MCP-1 release. At the same time, varying concentrations of chondroitin sulfate (CS) were added in a physiologically relevant range (10-200 µg/mL) to determine its impact on MCP-1 release...
2017: Biomarker Insights
https://www.readbyqxmd.com/read/28875605/fate-of-neutrophils-during-the-recovery-phase-of-ischemia-reperfusion-induced-acute-kidney-injury
#5
Wonyong Cho, Jie Young Song, Se Won Oh, Myung Gyu Kim, Yoon Sook Ko, Hee Yong Lee, Sang Kyung Jo
Effective clearance of inflammatory cells is required for resolution of inflammation. Here, we show in vivo evidence that apoptosis and reverse transendothelial migration (rTEM) are important mechanisms in eliminating neutrophils and facilitating recovery following ischemia/reperfusion injury (IRI) of the kidney. The clearance of neutrophils was delayed in the Bax knockout (KO)(BM) → wild-type (WT) chimera in which bone marrow derived cells are partially resistant to apoptosis, compared to WT(BM) → WT mice...
October 2017: Journal of Korean Medical Science
https://www.readbyqxmd.com/read/28875271/comparative-effects-of-schisandrin-a-b-and-c-on-acne-related-inflammation
#6
Miaomiao Guo, Faliang An, Xing Wei, Minhua Hong, Yanhua Lu
Inflammatory responses induced by Propionibacterium acnes are a major etiological factor in the pathogenesis of acne vulgaris. Schisandrin A, schisandrin B, and schisandrin C are the representative lignans of Schisandra chinensis (Turcz.) Baill. extract. Although anti-inflammatory effects of the lignans have been shown, their effects on acne-related inflammation caused by P. acnes have not been investigated and compared. We pretreated THP-1 human monocytic cells with 5, 10, and 20 μM schisandrin A, B, and C, and stimulated the cells with P...
September 5, 2017: Inflammation
https://www.readbyqxmd.com/read/28872362/ferulic-acid-but-not-alpha-lipoic-acid-effectively-protects-thp-1-derived-macrophages-from-oxidant-and-pro-inflammatory-response-to-lps
#7
Izabela Szulc-Kielbik, Michal Kielbik, Magdalena Klink
OBJECTIVE: The diet supplementation with antioxidants-rich products is a way to protect people from free radical-induced diseases. In this study, we compare the anti-inflammatory/antioxidant activity of two compounds available as supplements: alpha lipoic acid (ALA) and ferulic acid (FA). MATERIALS AND METHODS: The free radical scavenging capacity of ALA and FA in the cell free system was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Anti-inflammatory activity of both compounds was determined, in vitro, on THP-1 derived macrophages model, both resting (not stimulated) and inflammatory (lipopolysaccharide- or tumor-necrosis factor α-stimulated)...
September 5, 2017: Immunopharmacology and Immunotoxicology
https://www.readbyqxmd.com/read/28867672/phagocytosis-and-inflammation-exploring-the-effects-of-the-components-of-e-cigarette-vapor-on-macrophages
#8
Miranda P Ween, Jonathan J Whittall, Rhys Hamon, Paul N Reynolds, Sandra J Hodge
E-cigarettes are perceived as harmless; however, evidence of their safety is lacking. New data suggests E-cigarettes discharge a range of compounds capable of physiological damage to users. We previously established that cigarette smoke caused defective alveolar macrophage phagocytosis. The present study compared the effect E-cigarette of components; E-liquid flavors, nicotine, vegetable glycerine, and propylene glycol on phagocytosis, proinflammatory cytokine secretion, and phagocytic recognition molecule expression using differentiated THP-1 macrophages...
August 2017: Physiological Reports
https://www.readbyqxmd.com/read/28855441/microrna-182-promotes-lipoprotein-lipase-expression-and-atherogenesisby-targeting-histone-deacetylase-9-in-apolipoprotein-e-knockout-mice
#9
Hai-Peng Cheng, Duo Gong, Zhen-Wang Zhao, Ping-Ping He, Xiao-Hua Yu, Qiong Ye, Chong Huang, Xin Zhang, Ling-Yan Chen, Wei Xie, Min Zhang, Liang Li, Xiao-Dan Xia, Xin-Ping Ouyang, Yu-Lin Tan, Zong-Bao Wang, Guo-Ping Tian, Xi-Long Zheng, Wei-Dong Yin, Chao-Ke Tang
BACKGROUND: Lipoprotein lipase (LPL) expressed in macrophages plays an important role in promoting the development of atherosclerosis or atherogenesis. MicroRNA-182 (miR-182) is involved in the regulation of lipid metabolism and inflammation. However, it remains unclear how miR-182 regulates LPL and atherogenesis.Methods and Results:Using bioinformatics analyses and a dual-luciferase reporter assay, we identified histone deacetylase 9 (HDAC9) as a target gene of miR-182. Moreover, miR-182 upregulated LPL expression by directly targetingHDAC9in THP-1 macrophages...
August 29, 2017: Circulation Journal: Official Journal of the Japanese Circulation Society
https://www.readbyqxmd.com/read/28854426/autophagy-inhibition-contributes-to-ros-producing-nlrp3-dependent-inflammasome-activation-and-cytokine-secretion-in-high-glucose-induced-macrophages
#10
Jiezhi Dai, Xiaotian Zhang, Li Li, Hua Chen, Yimin Chai
BACKGROUND: Type 2 diabetes is a persistent inflammatory response that impairs the healing process. We hypothesized that stimulation with high glucose following a pro-inflammatory signal would lead to autophagy inhibition, reactive oxygen species (ROS) production and eventually to the activation of the Nod-like receptor protein (NLRP) -3. METHODS: Macrophages were isolated from human diabetic wound. We measured the expression of NLRP3, caspase1 and interleukin-1 beta (IL-1β) by western blot and real-time PCR, and the surface markers on cells by flow cytometry...
August 30, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28842601/a-neuropeptide-substance-p-directly-induces-tissue-repairing-m2-like-macrophages-by-activating-the-pi3k-akt-mtor-pathway-even-in-the-presence-of-ifn%C3%AE
#11
Ji Eun Lim, Eunkyung Chung, Youngsook Son
Macrophage polarization plays an important role in tissue damage and repair. In this study, we show that Substance-P (SP) can directly induce M2 polarization of inflammatory macrophages. SP induced the differentiation of GM-CSF-differentiated pro-inflammatory macrophages into alternatively activated phagocytic M2 like macrophages (M2(SP)) through direct activation of the PI3K/Akt/mTOR/S6kinase pathway and induction of Arginase-1, CD163, and CD206, all of which were nullified by pretreatment with the neurokinin-1 receptor (NK-1R) antagonist RP67580 and specific signaling pathway inhibitors...
August 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28837884/macrophages-induce-emt-to-promote-invasion-of-lung-cancer-cells-through-the-il-6-mediated-cox-2-pge2-%C3%AE-catenin-signalling-pathway
#12
Dehai Che, Shuai Zhang, Zihan Jing, Lihua Shang, Shi Jin, Fang Liu, Jing Shen, Yue Li, Jing Hu, Qingwei Meng, Yan Yu
Infiltration of macrophages plays a critical role in the connection between inflammation and cancer invasion; however, the molecular mechanism that enables this crosstalk remains unclear. This paper investigates a molecular link between infiltration of macrophages and metastasis of lung cancer cells. In this study, the macrophage density and cyclooxygenase-2 (COX-2) protein were examined in surgical specimens by immunohistochemistry (IHC), and the prostaglandin E2 (PGE2) levels were determined in the blood of 30 non-small cell lung cancer (NSCLC) patients using enzyme-linked immunosorbent assay (ELISA)...
August 21, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28837623/multidimensional-pooled-shrna-screens-in-human-thp-1-cells-identify-candidate-modulators-of-macrophage-polarization
#13
Ewa Surdziel, Ieuan Clay, Florian Nigsch, Anke Thiemeyer, Cyril Allard, Gregory Hoffman, John S Reece-Hoyes, Tanushree Phadke, Romain Gambert, Caroline Gubser Keller, Marie-Gabrielle Ludwig, Birgit Baumgarten, Mathias Frederiksen, Dirk Schübeler, Klaus Seuwen, Tewis Bouwmeester, Barna D Fodor
Macrophages are key cell types of the innate immune system regulating host defense, inflammation, tissue homeostasis and cancer. Within this functional spectrum diverse and often opposing phenotypes are displayed which are dictated by environmental clues and depend on highly plastic transcriptional programs. Among these the 'classical' (M1) and 'alternative' (M2) macrophage polarization phenotypes are the best characterized. Understanding macrophage polarization in humans may reveal novel therapeutic intervention possibilities for chronic inflammation, wound healing and cancer...
2017: PloS One
https://www.readbyqxmd.com/read/28822766/pseudomonas-aeruginosa-increases-muc1-expression-in-macrophages-through-the-tlr4-p38-pathway
#14
Kosuke Kato, Alec D Hanss, Marina A Zemskova, Nicole E Morgan, Marianne Kim, Kenneth S Knox, Yong Lin, Erik P Lillehoj, Kwang Chul Kim
Alveolar macrophages (AMs) play a critical role in the clearance of Pseudomonas aeruginosa (Pa) from the airways. However, hyper-activation of macrophages can impair bacterial clearance and contribute to morbidity and mortality. MUC1 mucin is a membrane-tethered, high molecular mass glycoprotein expressed on the apical surface of mucosal epithelial cells and some hematopoietic cells, including macrophages, where it counter-regulates inflammation. We recently reported that Pa up-regulates the expression of MUC1 in primary human AMs and THP-1 macrophages, and that increased MUC1 expression in these cells prevents hyper-activation of macrophages that appears to be important for host defense against severe pathology of Pa lung infection...
October 14, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28815730/loss-of-bmi-1-dampens-migration-and-emt-of-colorectal-cancer-in-inflammatory-microenvironment-through-tlr4-md-2-myd88-mediated-nf-%C3%AE%C2%BAb-signaling
#15
Kai Ye, Qi-Wei Chen, Ya-Feng Sun, Jian-An Lin, Jian-Hua Xu
Increasing evidence from various clinical and experimental studies has demonstrated that the inflammatory microenvironment created by immune cells facilitates tumor migration. Epithelial-mesenchymal transition (EMT) is involved in the progression of cancer invasion and metastasis in an inflammatory microenvironment. B-lymphoma Moloney murine leukemia virus insertion region 1 (BMI-1) acts as an oncogene in various tumors. Ectopic expression of Bmi-1 have an effect on EMT and invasiveness. The purpose of this study was to investigate the efficacy of BMI-1 on inflammation-induced tumor migration and EMT and the underlying mechanism...
August 16, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28807632/development-of-an-in-vitro-co-culture-model-to-mimic-the-human-intestine-in-healthy-and-diseased-state
#16
Angela A M Kämpfer, Patricia Urbán, Sabrina Gioria, Nilesh Kanase, Vicki Stone, Agnieszka Kinsner-Ovaskainen
The intestine forms the largest interface between the environment and the human organism. Its integrity and functioning are crucial for the uptake of nutrients while preventing access of harmful antigens. Inflammatory conditions can significantly change the normal functioning of the intestine. In vitro models that adequately reproduce both healthy and inflamed intestinal tissue could provide a useful tool for studying the mechanisms of intestinal inflammation and investigating new therapeutic drugs. We established a co-culture of Caco-2 and PMA-differentiated THP-1 cells that mimics the intestine in healthy and controlled inflamed states...
August 12, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28801727/inflammation-induced-by-candida-parapsilosis-in-thp-1-cells-and-human-peripheral-blood-mononuclear-cells-pbmcs
#17
Zhimin Duan, Xu Chen, Leilei Du, Caixia Liu, Rong Zeng, Qing Chen, Min Li
Candida parapsilosis is one of the most prevalent Candida species; however, the inflammation response induced by C. parapsilosis and related mechanism received few studies. In this study, we analyzed the pro-inflammatory cytokine responses evoked by C. parapsilosis in human peripheral blood mononuclear cells (PBMCs) and THP-1 cells, determined the signal pathways related to the inflammation response and investigated the expression of dectin-1 modified with C. parapsilosis. Exposure of PBMCs and THP-1 cells to C...
August 11, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28801561/inflammation-mediated-sod-2-upregulation-contributes-to-epithelial-mesenchymal-transition-and-migration-of-tumor-cells-in-aflatoxin-g1-induced-lung-adenocarcinoma
#18
Li Yi, Haitao Shen, Mei Zhao, Peilu Shao, Chunping Liu, Jinfeng Cui, Juan Wang, Can Wang, Ningfei Guo, Lifei Kang, Ping Lv, Lingxiao Xing, Xianghong Zhang
Tumor-associated inflammation plays a critical role in facilitating tumor growth, invasion and metastasis. Our previous study showed Aflatoxin G1 (AFG1) could induce lung adenocarcinoma in mice. Chronic lung inflammation associated with superoxide dismutase (SOD)-2 upregulation was found in the lung carcinogenesis. However, it is unclear whether tumor-associated inflammation mediates SOD-2 to contribute to cell invasion in AFG1-induced lung adenocarcinoma. Here, we found increased SOD-2 expression associated with vimentin, α-SMA, Twist1, and MMP upregulation in AFG1-induced lung adenocarcinoma...
August 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28782184/integrin-mediated-interactions-control-macrophage-polarization-in-3d-hydrogels
#19
Byung-Hyun Cha, Su Ryon Shin, Jeroen Leijten, Yi-Chen Li, Sonali Singh, Julie C Liu, Nasim Annabi, Reza Abdi, Mehmet R Dokmeci, Nihal Engin Vrana, Amir M Ghaemmaghami, Ali Khademhosseini
Adverse immune reactions prevent clinical translation of numerous implantable devices and materials. Although inflammation is an essential part of tissue regeneration, chronic inflammation ultimately leads to implant failure. In particular, macrophage polarity steers the microenvironment toward inflammation or wound healing via the induction of M1 and M2 macrophages, respectively. Here, this paper demonstrates that macrophage polarity within biomaterials can be controlled through integrin-mediated interactions between human monocytic THP-1 cells and collagen-derived matrix...
August 7, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28775237/c1q-tumor-necrosis-factor-related-protein-3-protects-macrophages-against-lps-induced-lipid-accumulation-inflammation-and-phenotype-transition-via-ppar%C3%AE-and-tlr4-mediated-pathways
#20
Jiale Lin, Qi Liu, Hui Zhang, Xingtao Huang, Ruoxi Zhang, Shuyuan Chen, Xuedong Wang, Bo Yu, Jingbo Hou
Macrophage inflammation and foam cell formation are critical events during the initiation and development of atherosclerosis (AS). C1q/tumor necrosis factor-related protein-3 (CTRP3) is a novel adipokine with anti-inflammatory and cardioprotection properties; however, little is known regarding the influence of CTRP3 on AS. As macrophages play a key role in AS, this study investigated the effects of CTRP3 on macrophage lipid metabolism, inflammatory reactions, and phenotype transition, as well as underlying mechanisms, to reveal the relationship between CTRP3 and AS...
July 28, 2017: Oncotarget
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