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https://www.readbyqxmd.com/read/28747771/different-potential-of-extracellular-vesicles-to-support-thrombin-generation-contributions-of-phosphatidylserine-tissue-factor-and-cellular-origin
#1
Carla Tripisciano, René Weiss, Tanja Eichhorn, Andreas Spittler, Thomas Heuser, Michael Bernhard Fischer, Viktoria Weber
Cells release diverse types of vesicles constitutively or in response to proliferation, injury, inflammation, or stress. Extracellular vesicles (EVs) are crucial in intercellular communication, and there is emerging evidence for their roles in inflammation, cancer, and thrombosis. We investigated the thrombogenicity of platelet-derived EVs, which constitute the majority of circulating EVs in human blood, and assessed the contributions of phosphatidylserine and tissue factor exposure on thrombin generation. Addition of platelet EVs to vesicle-free human plasma induced thrombin generation in a dose-dependent manner, which was efficiently inhibited by annexin V, but not by anti-tissue factor antibodies, indicating that it was primarily due to the exposure of phosphatidylserine on platelet EVs...
July 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28734155/activation-of-mtor-is-involved-in-anti-%C3%AE-2gpi-%C3%AE-2gpi-induced-expression-of-tissue-factor-and-il-8-in-monocytes
#2
Longfei Xia, Hong Zhou, Ting Wang, Yachao Xie, Ting Wang, Xiaoyan Wang, Jinchuan Yan
Previous study has demonstrated that activation of the mammalian target of rapamycin (mTOR) pathway in endothelial cells (ECs) results in the formation of chronic vascular lesions associated with antiphospholipid syndrome (APS). In addition, it has been shown that stimulation of monocytes and ECs by antiphospholipid antibodies (aPL) leads to a prothrombotic and proinflammatory state and up-regulated expression of tissue factor (TF) and inflammatory cytokines. However, the role of mTOR in pathogenic mechanisms of APS remains largely unexplored...
June 1, 2017: Thrombosis Research
https://www.readbyqxmd.com/read/28728011/anti-inflammatory-effect-of-sugar-amino-acid-maillard-reaction-products-on-intestinal-inflammation-model-in%C3%A2-vitro-and-in%C3%A2-vivo
#3
Jun-Gu Oh, Su-Hyun Chun, Da Hyun Kim, Jin Hye Kim, Hye Soo Shin, Yong Soo Cho, Yong Ki Kim, Hee-Don Choi, Kwang-Won Lee
The Maillard reaction is a nonenzymatic reaction between an amino acid and a reducing sugar that usually occurs upon heating. This reaction occurs routinely in cooking, generates numerous products, which are collectively referred to as Maillard reaction products (MRPs) contributing to aroma and color features. Advanced glycation end-products (AGEs) transformed from MRPs are participated in many types of inflammation reaction. In this study, various sugar-amino acid MRPs were prepared from three different amino acids (lysine, arginine, and glycine) and sugars (glucose, fructose, and galactose) for 1 h with heating at 121 °C...
July 11, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28713783/streptococcus-pyogenes-phospholipase-a2-induces-the-expression-of-adhesion-molecules-on-human-umbilical-vein-endothelial-cells-and-aorta-of-mice
#4
Masataka Oda, Hisanori Domon, Mie Kurosawa, Toshihito Isono, Tomoki Maekawa, Masaya Yamaguchi, Shigetada Kawabata, Yutaka Terao
The Streptococcus pyogenes phospholipase A2 (SlaA) gene is highly conserved in the M3 serotype of group A S. pyogenes, which often involves hypervirulent clones. However, the role of SlaA in S. pyogenes pathogenesis is unclear. Herein, we report that SlaA induces the expression of intercellular adhesion molecule 1 (ICAM1) and vascular cell adhesion molecule 1 (VCAM1) via the arachidonic acid signaling cascade. Notably, recombinant SlaA induced ICAM1 and VCAM1 expression in human umbilical vein endothelial cells (HUVECs), resulting in enhanced adhesion of human monocytic leukemia (THP-1) cells...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28709982/impact-of-zinc-oxide-nanoparticles-on-an-in-vitro-model-of-the-human-air-blood-barrier
#5
Rossella Bengalli, Maurizio Gualtieri, Laura Capasso, Chiara Urani, Marina Camatini
The inhalation of zinc oxide nanoparticles (nZnO) may induce systemic diseases, damages to the alveolar epithelium and inflammatory response to endothelial cells. In this work the use of an in vitro air-blood barrier (ABB) model provided a tool to elucidate the biological mechanisms underlying the potential effects of inhaled nanoparticles (NPs). The ABB model used is composed of a Transwell co-culture of a lung epithelial cell line (NCI-H441) and an immortalized pulmonary microvascular endothelial cell line (HPMEC-ST1...
July 11, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28699129/shikonin-changes-the-lipopolysaccharide-induced-expression-of-inflammation-related-genes-in-macrophages
#6
Lucia Satiko Yoshida, Tomohito Kakegawa, Yasukatsu Yuda, Hiromi Takano-Ohmuro
We aimed to find candidate molecules possibly involved in the anti-inflammatory activity of shikonin (active compound of "Shikon") by analyzing its effects on gene expression of lipopolysaccharide (LPS)-treated THP-1 macrophages. Polysome-associated mRNAs (those expected to be under translation: translatome) from cells treated with LPS alone (LPS: 5 µg/mL), shikonin alone (S: 100 nM), or LPS plus shikonin (LPS&S) for 3 h were analyzed by DNA microarray followed by detection of enriched pathways/gene ontologies using the tools of the STRING database...
July 11, 2017: Journal of Natural Medicines
https://www.readbyqxmd.com/read/28697402/functional-biocompatibility-testing-of-silicone-breast-implants-and-a-novel-classification-system-based-on-surface-roughness
#7
S Barr, E W Hill, A Bayat
PURPOSE: Increasing numbers of women undergo breast implantation for cosmetic and reconstructive purposes. Contracture of the fibrous capsule, which encases the implant leads to significant pain and reoperation. Texture, wettability and the cellular reaction to implant surfaces are poorly understood determinants of implant biocompatibility. The aim of this study was to evaluate the in-vitro characteristics of a range of commercial available implants using a macrophage based assay of implant biocompatibility and a quantitative assessment of wettability and texture...
June 27, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28696794/the-effects-of-asthma-medications-on-reactive-oxygen-species-production-in-human-monocytes
#8
Ming-Kai Tsai, Yi-Ching Lin, Ming-Yii Huang, Min-Sheng Lee, Chang-Hung Kuo, Po-Lin Kuo, Ching-Hsiung Lin, Chih-Hsing Hung
OBJECTIVE: Asthma is a chronic inflammatory airway disease induced by many environmental factors. The inhalation of allergens and pollutants promote the reactive oxygen species (ROS) production leading to airway inflammation, hyper-responsiveness and remodeling in allergic asthma. The effects of asthma medications on ROS production are unclear. The present study investigated the anti-ROS effects of current asthma medications including inhaled corticosteroid (ICS; budesonide and fluticasone), leukotriene receptor antagonist (LTRA; montelukast), long acting β2 agonists (LABAs; salmeterol and formoterol) and a new extra-LABA (indacaterol)...
July 11, 2017: Journal of Asthma: Official Journal of the Association for the Care of Asthma
https://www.readbyqxmd.com/read/28691370/bioaccessibility-bioavailability-and-anti-inflammatory-effects-of-anthocyanins-from-purple-root-vegetables-using-mono-and-co-culture-cell-models
#9
Hua Zhang, Hassan Yousef, Justin Renaud, Ronghua Liu, Cheng Yang, Yong Sun, Rong Tsao
SCOPE: Immune-inflammatory, signalling and metabolic effects are the main pillars for bioactivity of anthocyanins derived from highly pigmented root vegetables. This study aims to assess the bioaccessibility and bioavailability of purple carrot and potato derived anthocyanins and the molecular mechanisms of their ability to ameliorate cellular inflammation in a mono- and co-culture cell models. METHODS AND RESULTS: An in vitro gastrointestinal model was used and demonstrated bioaccessibility of 44...
July 10, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28678312/identification-of-mirnas-as-atherosclerosis-biomarkers-and-functional-role-of-mir-126-in-atherosclerosis-progression-through-mapk-signalling-pathway
#10
X-Z Hao, H-M Fan
OBJECTIVE: Mounting evidence suggests the role of microRNAs (miRNAs) in regulating inflammatory responses in various vascular diseases. Inflammation is the key mechanism leading to atherosclerosis (AS) and various miRNAs are aberrantly expressed in response to AS pathophysiology. However, there are very limited studies that serve to elucidate the role of specific miRNA in in vivo or in vitro AS models. MATERIALS AND METHODS: Microarray analysis of blood plasma of apolipoprotein deficient (apoE-/-) mice was performed followed by the confirmation using qPCR...
June 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28665518/increased-expression-of-tipe2-in-alternatively-activated-macrophages-is-associated-with-eosinophilic-inflammation-and-disease-severity-in-chronic-rhinosinusitis-with-nasal-polyps
#11
Yin Yao, Zhi-Chao Wang, Jin-Xin Liu, Jin Ma, Cai-Ling Chen, Yi-Ke Deng, Bo Liao, Nan Wang, Heng Wang, Qin Ning, Zheng Liu
BACKGROUND: Chronic rhinosinusitis with nasal polyps (CRSwNP) is a multifactorial disorder characterized by exaggerated local immune responses. Tumor necrosis factor-α-induced protein 8-like 2 (TIPE2) is a novel protein with potential immune modulating function. The expression and function of TIPE2 in human airway diseases are unclear. METHODS: The expression of TIPE2 in sinonasal mucosal samples was assessed by means of quantitative reverse transcript-polymerse chain reaction, immunohistochemistry, and Western blotting...
June 30, 2017: International Forum of Allergy & Rhinology
https://www.readbyqxmd.com/read/28662496/macrophage-inflammatory-protein-2-in-high-mobility-group-box-1-secretion-of-macrophage-cells-exposed-to-lipopolysaccharide
#12
Qin Chaochao, Guohua Lou, Ying Yang, Yanning Liu, Ying Hu, Zheng Min, Ping Chen, Jiliang He, Zhi Chen
BACKGROUND/AIMS: Macrophage inflammatory protein-2 (MIP-2), a type of leukocyte chemokine, is primarily produced by macrophages, and levels increase significantly in early inflammation. However, the precise biological functions and mechanisms of MIP-2 in the development of inflammation remain unclear. The purposes of the present study were to investigate the role of MIP-2 in inflammation induced by lipopolysaccharide (LPS) in vitro and to determine the possibility of blocking the high mobility group box 1 (HMGB1) signalling pathway via MIP-2 inhibition...
June 26, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28662100/mir-146a-mir-146b-and-mir-155-increase-expression-of-il-6-and-il-8-and-support-hsp10-in-an-in-vitro-sepsis-model
#13
Dagmar Pfeiffer, Eva Roßmanith, Ingrid Lang, Dieter Falkenhagen
microRNAs (miRNAs) play an essential role in inflammation processes including sepsis. This study aimed to identify miRNAs as candidates for therapies that are involved in the innate immune response and to assess their potential functions in the activation of the endothelium. We stimulated THP-1 monocytes with 10 ng/ml LPS for 4 h and used the supernatant for the stimulation of human umbilical vein endothelial cells (HUVEC) or human pulmonary microvascular endothelial cells (HPMEC) for 16 h. miRNA array analysis (of 1,891 miRNAs) identified a 1...
2017: PloS One
https://www.readbyqxmd.com/read/28661045/sodium-salicylate-modulates-inflammatory-responses-through-amp-activated-protein-kinase-activation-in-lps-stimulated-thp-1-cells
#14
Weiwei Bao, Yaru Luo, Dan Wang, Jian Li, Xi Wu, Wei Mei
Sodium salicylate (NaSal) is a nonsteroidal anti-inflammatory drug. The putative mechanisms for NaSal's pharmacologic actions include the inhibition of cyclooxygenases, platelet-derived thromboxane A2 and NF-κB signaling. Recent studies demonstrated that salicylate could activate AMP-activated protein kinase (AMPK), an energy sensor that maintains the balance between ATP production and consumption. The anti-inflammatory action of AMPK has been reported to be mediated by promoting mitochondrial biogenesis and fatty acid oxidation...
June 29, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28659229/homeopathic-potencies-of-arnica-montana-l-change-gene-expression-in-a-tamm-horsfall-protein-1-cell-line-in-vitro-model-the-role-of-ethanol-as-a-possible-confounder-and-statistical-bias
#15
Salvatore Chirumbolo, Geir Bjørklund
Marzotto et al. showed that homeopathic preparations of Arnica montana L. acted directly on gene expression of Tamm-Horsfall protein-1 (THP-1) monocyte/macrophage cell lines activated with phorbol12-myristate13-acetate and interleukin-4 (IL-4). A. montana homeopathic dilutions are used in complementary and alternative medicine to treat inflammation disorders and post-traumatic events as well as for wound repair. The French Pharmacopoeia of these remedies uses 0.3% ethanol in each centesimal dilution. In this paper, we discuss how ethanol-containing A...
July 2017: Journal of Integrative Medicine
https://www.readbyqxmd.com/read/28659178/azithromycin-decreases-nalp3-mrna-stability-in-monocytes-to-limit-inflammasome-dependent-inflammation
#16
Elizabeth A Lendermon, Tiffany A Coon, Joseph S Bednash, Nathaniel M Weathington, John F McDyer, Rama K Mallampalli
BACKGROUND: Azithromycin, an antibiotic used for multiple infectious disorders, exhibits anti-inflammatory effects, but the molecular basis for this activity is not well characterized. Azithromycin inhibits IL-1β-mediated inflammation that is dependent, in part, on inflammasome activity. Here, we investigated the effects of azithromycin on the NACHT, LRR, and PYD domains-containing protein 3 (NALP3) protein, which is the sensing component of the NALP3 inflammasome, in human monocytes...
June 28, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28653238/ampk-nf-%C3%AE%C2%BAb-signaling-pathway-regulated-by-ghrelin-participates-in-the-regulation-of-huvec-and-thp1-inflammation
#17
Min Zhang, Shuping Wang, Zhicheng Pan, Tiantong Ou, Jianwei Ma, Hua Liu, Ruogu Li, Ping Yang, Wenzheng Han, Shaofeng Guan, Xumin Hou, Weiyi Fang, Xinkai Qu
Endothelial inflammation and monocyte plays an essential role in the initiation and progression of atherosclerosis. Ghrelin is beneficial for atherosclerosis progression. However, the detailed and precise molecular mechanisms of how ghrelin regulates endothelial inflammation are not clear. In this study, we investigated the regulation mechanism of ghrelin on TNF-α-activated endothelial inflammation and monocyte adhesion. It was found that TNF-α-induced monocyte adhesion on HUVEC was significantly attenuated by ghrelin...
June 26, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28646516/tofacitinib-restores-the-inhibition-of-reverse-cholesterol-transport-induced-by-inflammation-understanding-the-lipid-paradox-associated-with-rheumatoid-arthritis
#18
S Pérez-Baos, J I Barrasa, P Gratal, A Larrañaga-Vera, I Prieto-Potin, G Herrero-Beaumont, R Largo
BACKGROUND AND PURPOSE: Patients with active rheumatoid arthritis (RA) have increased cardiovascular mortality, paradoxically associated with reduced circulating lipid levels. The Janus kinase (Jak) inhibitor tofacitinib ameliorates systemic and joint inflammation in RA with a concomitant increase in serum lipids. We analyzed the effect of tofacitinib on the lipid profile of hyperlipidemic rabbits with chronic arthritis (CA) and on the regulation of reverse cholesterol transport (RCT) during chronic inflammation...
June 23, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28643827/anti-oxidant-and-immune-modulatory-properties-of-sulfated-alginate-derivatives-on-human-chondrocytes-and-macrophages
#19
Anne Kerschenmeyer, Øystein Arlov, Vera Malheiro, Matthias Steinwachs, Markus Rottmar, Katharina Maniura-Weber, Gemma Palazzolo, Marcy Zenobi-Wong
Degeneration of articular cartilage represents one of the most common causes of pain and disability in our aging society. Current treatments only address the symptoms of joint disease, but not their underlying causes which include oxidative stress and inflammation in cartilage and surrounding tissues. Sulfated biopolymers that mimic aspects of the native extracellular environment of cartilage are recently gaining interest as a means to slow the inflammatory events responsible for tissue degeneration. Here we show that the natural polysaccharide alginate and particularly its sulfated derivatives have potent anti-oxidant, anti-inflammatory and anti-immunogenic properties in vitro...
June 23, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28642135/light-is-increased-in-patients-with-coronary-disease-and-regulates-inflammatory-response-and-lipid-metabolism-in-oxldl-induced-thp-1-macrophages
#20
Xiaomei Yuan, Yonglin Gu, Xiaoyu Lai, Qing Gu
Inflammation is critical for the progression of hyperlipidemia. Although the exact mechanism through which inflammation affects hyperlipidemia is not very clear, evidence suggests that the tumor necrosis factor superfamily member 14 (TNFSF14/LIGHT)LIGHT might regulate lipid metabolism. In this study we investigated the expression of LIGHT in patients with different stages of coronary disease. The expression of lipid metabolism-related enzymes and inflammation-related proteins were further explored in oxidized low-density lipoproteins (oxLDL)-induced THP-1 macrophages...
June 19, 2017: Biochemical and Biophysical Research Communications
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