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https://www.readbyqxmd.com/read/28100701/self-cytoplasmic-dna-upregulates-the-mutator-enzyme-apobec3a-leading-to-chromosomal-dna-damage
#1
Rodolphe Suspène, Bianka Mussil, Hélène Laude, Vincent Caval, Noémie Berry, Mohamed S Bouzidi, Valérie Thiers, Simon Wain-Hobson, Jean-Pierre Vartanian
Foreign and self-cytoplasmic DNA are recognized by numerous DNA sensor molecules leading to the production of type I interferons. Such DNA agonists should be degraded otherwise cells would be chronically stressed. Most human APOBEC3 cytidine deaminases can initiate catabolism of cytoplasmic mitochondrial DNA. Using the human myeloid cell line THP-1 with an interferon inducible APOBEC3A gene, we show that cytoplasmic DNA triggers interferon α and β production through the RNA polymerase III transcription/RIG-I pathway leading to massive upregulation of APOBEC3A By catalyzing C→U editing in single stranded DNA fragments, the enzyme prevents them from re-annealing so attenuating the danger signal...
January 18, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28088611/lactobacillus-paracasei-modulates-lps-induced-inflammatory-cytokine-release-by-monocyte-macrophages-via-the-up-regulation-of-negative-regulators-of-nf-kappab-signaling-in-a-tlr2-dependent-manner
#2
Ke-Yi Sun, Dong-Hua Xu, Chao Xie, Susan Plummer, James Tang, Xiao Fan Yang, Xiao Hui Ji
The application of the probiotic lactobacillus is suggested in the treatment of some inflammatory diseases of intestines due to its potential ability to attenuate inflammation. However, the mechanism is not completely understood. In PBMCs, Lactobacillus paracasei (L. Paracasei) down-regulated the LPS-induced production of TNF-α and IL-6. Using a macrophage-like differentiated THP-1 cell line induced by PMA, we investigated the effect of L. paracasei on the production of pro-inflammatory cytokines by monocyte-macrophages...
January 12, 2017: Cytokine
https://www.readbyqxmd.com/read/28079123/trim22-mediated-apoptosis-is-associated-with-bak-oligomerization-in-monocytes
#3
Chi Chen, DongYan Zhao, Shu Fang, QiXing Chen, BaoLi Cheng, XiangMing Fang, Qiang Shu
Monocyte apoptosis is a key mechanism that orchestrates host immune responses during sepsis. TRIM22 is constitutively expressed at high levels in monocytes and plays important roles in the antiviral response and inflammation. Overexpression of TRIM22 interferes with the clonogenic growth of monocytic cells, suggesting that TRIM22 may regulate monocyte survival. However, the effect of TRIM22 on monocyte apoptosis remains unknown. In the present report, lipopolysaccharides (LPS)-primed human peripheral blood monocytes expressing higher levels of TRIM22 were more sensitive to apoptosis...
January 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28071973/collagen-cross-linking-biophysical-biochemical-and-biological-response-analysis
#4
L Delgado, Kieran Fuller, Dimitrios I Zeugolis
Extracted forms of collagen are subjected to chemical cross-linking to enhance their stability. However, traditional cross-linking approaches are associated with toxicity and inflammation. This work investigates the stabilisation capacity, cytotoxicity and inflammatory response of collagen scaffolds cross-linked with glutaraldehyde, carbodiimide, 4-arm polyethylene glycol succinimidyl glutarate, genipin and oleuropein. Although all cross-linking methods reduced free amine groups, variable data were obtained with respect to denaturation temperature, resistance to collagenase digestion and mechanical properties...
January 10, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28049625/regulation-of-the-macrophage-oxytocin-receptor-in-response-to-inflammation
#5
Angela Szeto, Ni Sun-Suslow, Armando J Mendez, Rosa I Hernandez, Klaus V Wagner, Philip M McCabe
It has been demonstrated that the neuropeptide oxytocin (OT) attenuates oxidative stress and inflammation in macrophages. In the current study, we examined the role of inflammation on the expression of the oxytocin receptor (OXTR). We hypothesized that OXTR expression is increased during the inflammation though a nuclear factor kappa B (NF-κB) mediated pathway, thus responding as an acute phase protein. Inflammation was induced by treating macrophages (human primary, THP-1, and murine) with lipopolysaccharide (LPS) and monitored by expression of IL-6...
January 3, 2017: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28011195/protective-role-of-klotho-protein-on-epithelial-cells-upon-co-culture-with-activated-or-senescent-monocytes
#6
Jennifer Mytych, Izabela Wos, Przemyslaw Solek, Marek Koziorowski
Monocytes ensure proper functioning and maintenance of epithelial cells, while good condition of monocytes is a key factor of these interactions. Although, it was shown that in some circumstances, a population of altered monocytes may appear, there is no data regarding their effect on epithelial cells. In this study, using direct co-culture model with LPS-activated and Dox-induced senescent THP-1 monocytes, we reported for the first time ROS-induced DNA damage, reduced metabolic activity, proliferation inhibition and cell cycle arrest followed by p16-, p21- and p27-mediated DNA damage response pathways activation, premature senescence and apoptosis induction in HeLa cells...
December 21, 2016: Experimental Cell Research
https://www.readbyqxmd.com/read/27988459/suppression-of-lps-induced-nf-%C3%AE%C2%BAb-activity-in-macrophages-by-the-synthetic-aurone-z-2-5-hydroxymethyl-furan-2-yl-methylene-benzofuran-3-2h-one
#7
Hyo S Park, David E Nelson, Zachary E Taylor, James B Hayes, Kirsten D Cunningham, Brock A Arivett, Rajarshi Ghosh, Larissa C Wolf, Kimberley M Taylor, Mary B Farone, Scott T Handy, Anthony L Farone
Suppressing cytokine responses has frequently been shown to have promising therapeutic effects for many chronic inflammatory and autoimmune diseases. However, the severe side effects associated with the long-term use of current treatments, such as allergic reactions and increased risk of stroke, have focused attention towards the targeting of intracellular signaling mechanisms, such as NF-κB, that regulate inflammation. We synthesized a series of non-natural aurone derivatives and investigated their ability to suppress pro-inflammatory signaling in human monocyte (THP-1) and murine macrophage-like (RAW 267...
February 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/27959437/traditional-medicine-sobokchukeo%C3%A2-tang-modulates-the-inflammatory-response-in-adipocytes-and-macrophages
#8
Hoyoung Lee, Eun Hyoung Shim, Myeong Soo Lee, Chang-Seon Myung
Sobokchukeo-Tang (ST) is a well-known formula that is used for treating primary dysmenorrhea caused by blood stasis syndrome (BSS) in Korea and China. The current study investigated the anti‑inflammatory and anti‑adipogenesis effects of ST on adipocytes and macrophages. The anti‑inflammatory efficacy of ST was evaluated in RAW 264.7 cells and differentiated THP‑1 cells. To induce inflammation, the cells were treated with lipopolysaccharide (LPS; 1 µg/ml). Following the induction of inflammation, the levels of proinflammatory cytokines, interleukin‑6 (IL‑6) and tumor necrosis factor‑α (TNF‑α) in the cell supernatant were detected using enzyme‑linked immunosorbent assay...
January 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27959409/galectin-3-inhibition-ameliorates-hypoxia-induced-pulmonary-artery-hypertension
#9
Mingwen Hao, Miaomiao Li, Wenjun Li
Galectin-3 (Gal-3) is a β-galactoside-binding lectin, which is important in inflammation, fibrosis and heart failure. The present study aimed to investigate the role and mechanism of Gal-3 in hypoxia-induced pulmonary arterial hypertension (PAH). Male C57BL/6J and Gal‑3‑/‑ mice were exposed to hypoxia, then the right ventricular systolic pressure (RVSP) and Fulton's index were measured, and Gal‑3 mRNA and protein expression in the pulmonary arteries was analyzed by reverse transcription‑quantitative polymerase chain reaction and western blotting...
January 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27940550/cox-2-dependent-and-independent-effects-of-cox-2-inhibitors-and-nsaids-on-proatherogenic-changes-in-human-monocytes-macrophages
#10
Iryna Voloshyna, Lora J Kasselman, Steven E Carsons, Michael J Littlefield, Irving H Gomolin, Joshua De Leon, Allison B Reiss
It is the second decade of controversy regarding the cardiovascular effects of cyclo-oxygenase-2 (COX-2) inhibitors. At this time, celecoxib is the only available COX-2-specific inhibitor for treatment of pain and inflammation. Therefore, the present study was designed primarily to determine the impact of celecoxib on cholesterol handling (uptake via scavenger receptors and efflux from the cells) and foam cell formation in human THP-1 macrophages, followed by comparison to rofecoxib and other non-steroidal anti-inflammatory drugs (NSAIDs)...
December 9, 2016: Journal of Investigative Medicine: the Official Publication of the American Federation for Clinical Research
https://www.readbyqxmd.com/read/27935022/antiatherosclerotic-effect-of-ang-1-7-non-peptide-mimetic-ave-0991-is-mediated-by-inhibition-of-perivascular-and-plaque-inflammation-in-early-atherosclerosis
#11
D S Skiba, R Nosalski, T P Mikolajczyk, M Siedlinski, F J Rios, A C Montezano, J Jawien, R Olszanecki, R Korbut, M Czesnikiewicz-Guzik, R M Touyz, T J Guzik
BACKGROUND AND PURPOSE: Inflammation plays a key role in atherosclerosis. A protective role of angiotensin-(1-7) in vascular pathologies opened a possibility for therapeutic use of small molecule non-peptide Ang-(1-7) mimetics, such as AVE0991. The mechanisms of these vaso-protective effects of a Mas receptor agonist, AVE0991, remain unclear. EXPERIMENTAL APPROACH: We investigated the effects of AVE0991 on the spontaneous atherosclerosis in ApoE-/- mice, in the context of vascular inflammation and plaque stability...
December 9, 2016: British Journal of Pharmacology
https://www.readbyqxmd.com/read/27919268/air-liquid-interface-exposure-to-aerosols-of-poorly-soluble-nanomaterials-induces-different-biological-activation-levels-compared-to-exposure-to-suspensions
#12
Thomas Loret, Emmanuel Peyret, Marielle Dubreuil, Olivier Aguerre-Chariol, Christophe Bressot, Olivier le Bihan, Tanguy Amodeo, Bénédicte Trouiller, Anne Braun, Christophe Egles, Ghislaine Lacroix
BACKGROUND: Recently, much progress has been made to develop more physiologic in vitro models of the respiratory system and improve in vitro simulation of particle exposure through inhalation. Nevertheless, the field of nanotoxicology still suffers from a lack of relevant in vitro models and exposure methods to predict accurately the effects observed in vivo, especially after respiratory exposure. In this context, the aim of our study was to evaluate if exposing pulmonary cells at the air-liquid interface to aerosols of inhalable and poorly soluble nanomaterials generates different toxicity patterns and/or biological activation levels compared to classic submerged exposures to suspensions...
November 3, 2016: Particle and Fibre Toxicology
https://www.readbyqxmd.com/read/27910925/thrombomodulin-regulates-monocye-differentiation-via-pkc%C3%AE-and-erk1-2-pathway-in-vitro-and-in-atherosclerotic-artery
#13
Chien-Sung Tsai, Yi-Wen Lin, Chun-Yao Huang, Chun-Min Shih, Yi-Ting Tsai, Nai-Wen Tsao, Chin-Sheng Lin, Chun-Che Shih, Hellen Jeng, Feng-Yen Lin
Thrombomodulin (TM) modulates the activation of protein C and coagulation. Additionally, TM regulates monocyte migration and inflammation. However, its role on monocyte differentiation is still unknown. We investigated the effects of TM on monocyte differentiation. First, we found that TM was increased when THP-1 cells were treated with phorbol-12-myristate-13-acetate (PMA). Overexpression of TM enhanced the macrophage markers, CD14 and CD68 expression in PMA-induced THP-1. TM siRNA depressed the PMA-induced increase of p21(Cip1/WAF1) via ERK1/2-NF-kB p65 signaling...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27903990/baicalein-reduces-angiogenesis-in-the-inflammatory-microenvironment-via-inhibiting-the-expression-of-ap-1
#14
Yujie Huang, Zhaorui Miao, Yang Hu, Yang Yuan, Yuxin Zhou, Libin Wei, Kai Zhao, Qinglong Guo, Na Lu
Increasing clinical and experimental studies have demonstrated that refractory chronic inflammation will result in malignant tumor and anti-angiogenic therapy may be an effective way to thwart the progression. Baicalein, one of the major active flavanoids found in Scutellaria baicalensis Georgi, has been exhibited potent anti-inflammation and anti-tumor effects by reducing angiogenesis. However, the exact mechanism of baicalein on endothelial cells in inflammatory microenvironment was not clear yet. Here, we investigated the anti-angiogenic effect of baicalein by incubating human umbilical vein endothelial cells (HUVECs) with THP-1 conditioned medium in vitro...
November 26, 2016: Oncotarget
https://www.readbyqxmd.com/read/27902980/gene-expression-profiling-and-functional-characterization-of-macrophages-in-response-to-circulatory-microparticles-produced-during-trypanosoma-cruzi-infection-and-chagas-disease
#15
Imran H Chowdhury, Sue-Jie Koo, Shivali Gupta, Lisa Yi Liang, Bojlul Bahar, Laura Silla, Julio Nuñez-Burgos, Natalia Barrientos, Maria Paola Zago, Nisha Jain Garg
BACKGROUND: Chronic inflammation and oxidative stress are hallmarks of chagasic cardiomyopathy (CCM). In this study, we determined if microparticles (MPs) generated during Trypanosoma cruzi (Tc) infection carry the host's signature of the inflammatory/oxidative state and provide information regarding the progression of clinical disease. METHODS: MPs were harvested from supernatants of human peripheral blood mononuclear cells in vitro incubated with Tc (control: LPS treated), plasma of seropositive humans with a clinically asymptomatic (CA) or symptomatic (CS) disease state (vs...
December 1, 2016: Journal of Innate Immunity
https://www.readbyqxmd.com/read/27888719/treponema-pallidum-flagellin-flaa2-induces-il-6-secretion-in-thp-1-cells-via-the-toll-like-receptor-2-signaling-pathway
#16
Yafeng Xie, Man Xu, Yongjian Xiao, Zhuoran Liu, Chuanhao Jiang, Xingxing Kuang, Chuan Wang, Haiying Wu, Jing Peng, Chun Li, Yu Wang, Huaming Liu, Bin Liu, Xiaotuan Zhang, Feijun Zhao, Tiebing Zeng, Shuangquan Liu, Yimou Wu
Treponema pallidum subsp. pallidum membrane proteins are considered as potent inducers in the initiation and development of inflammation. In the present study, the mechanism that leads to the production of interleukin 6 (IL-6), one of the key proinflammatory cytokines, by human monocytic THP-1 cells when these cells are treated with T. pallidum flagellin FlaA2 was investigated. Stimulation with flagellin FlaA2 can induce IL-6 expression in human monocytes and augment the phosphorylation of ERK, p38, and NF-κB, but has no effect on the phosphorylation of JNK...
January 2017: Molecular Immunology
https://www.readbyqxmd.com/read/27881852/macrophage-derived-exosomes-induce-inflammatory-factors-in-endothelial-cells-under-hypertensive-conditions
#17
Mayuko Osada-Oka, Masayuki Shiota, Yasukatsu Izumi, Masaki Nishiyama, Masako Tanaka, Takehiro Yamaguchi, Emi Sakurai, Katsuyuki Miura, Hiroshi Iwao
Hypertension is one of the most important cardiovascular risk factors and results in macrophage infiltration of blood vessels. However, how macrophages coordinate inflammatory responses with endothelial cells (ECs) remains unclear. In this study, we investigated whether exosomes upregulate the expression of inflammatory factors in ECs under hypertensive conditions. Hypertension was induced in rats by continuous infusion of angiotensin II (Ang II). Exosomes were purified from rat serum by density gradient and ultracentrifugation and used to stimulate human coronary artery ECs (HCAECs)...
November 24, 2016: Hypertension Research: Official Journal of the Japanese Society of Hypertension
https://www.readbyqxmd.com/read/27878240/sirt1-activator-represses-the-transcription-of-tnf%C3%A2-%C3%AE-in-thp%C3%A2-1-cells-of-a-sepsis-model-via-deacetylation-of-h4k16
#18
Guo-Dong Chen, Wei-Dong Yu, Xiao-Ping Chen
Sepsis is a systemic inflammatory response resulting from the excessive production of pro-inflammatory cytokines, including tumor necrosis factor (TNF)‑α. Sirtuin 1 (SirT1) actively deacetylates histone proteins, and facilitates chromatin compaction and gene silencing. In the present study, a cell model of sepsis, comprising lipopolysaccharide (LPS)‑tolerant THP‑1 cells, was used to investigate whether the SirT1 activator, resveratrol, repressed the transcription of TNF‑α. Chromatin immunoprecipitation and real‑time PCR were used to determine the transcription of the TNF‑α promoter...
December 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27863506/interleukin-37-limits-monosodium-urate-crystal-induced-innate-immune-responses-in-human-and-murine-models-of-gout
#19
Lei Liu, Yu Xue, Yingfeng Zhu, Dandan Xuan, Xue Yang, Minrui Liang, Juan Wang, Xiaoxia Zhu, Jiong Zhang, Hejian Zou
BACKGROUND: Interleukin (IL)-37 has emerged as a fundamental inhibitor of innate immunity. Acute gout is a self-limiting inflammatory response to monosodium urate (MSU) crystals. In the current study, we assessed the preventive and therapeutic effect of recombinant human IL-37 (rhIL-37) in human and murine gout models. METHODS: We investigated the expression of IL-37 in patients with active and inactive gouty arthritis and assessed the effect of rhIL-37 in human and murine gout models: a human monocyte cell line (THP-1) and human synovial cells (containing macrophage-like and fibroblast-like synoviocytes) exposed to MSU crystals, a peritoneal murine model of gout and a murine gouty arthritis model...
November 18, 2016: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/27862064/cytotoxicity-oxidative-stress-and-inflammation-induced-by-zno-nanoparticles-in-endothelial-cells-interaction-with-palmitate-or-lipopolysaccharide
#20
Yu Gong, Yuejia Ji, Fang Liu, Juan Li, Yi Cao
Recent studies showed that ZnO nanoparticles (NPs) might induce the toxicity to human endothelial cells. However, little is known about the interaction between ZnO NPs and circulatory components, which is likely to occur when NPs enter the blood. In this study, we evaluated ZnO NP-induced cytotoxicity, oxidative stress and inflammation in human umbilical vein endothelial cells (HUVECs), with the emphasis on the interaction with palmitate (PA) or lipopolysaccharide (LPS), because PA and LPS are normal components in human blood that increase in metabolic diseases...
November 15, 2016: Journal of Applied Toxicology: JAT
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