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V Balugo-López, E Hernández García de la Barrera, J Sastre
No abstract text is available yet for this article.
October 2016: Journal of Investigational Allergology & Clinical Immunology
Laura Bouché, Maria Panico, Paul Hitchen, Daniel Binet, Federico Sastre, Alexandra Faulds-Pain, Esmeralda Valiente, Evgeny Vinogradov, Annie Aubry, Kelly Fulton, Susan Twine, Susan M Logan, Brendan W Wren, Anne Dell, Howard R Morris
Glycosylation of flagellins is a well recognized property of many bacterial species. In this study we describe the structural characterization of novel flagellar glycans from a number of hypervirulent strains of C. difficile. We used mass spectrometry (nano LC- MS and MS/MS analysis) to identify a number of putative glycopeptides which carried a variety of glycoform substitutions each of which was linked through an initial HexNAc residue to Ser or Thr. Detailed analysis of a LLDGSSTEIR glycopeptide released by tryptic digestion, which carried two variant structures, revealed that the glycopeptide contained, in addition to carbohydrate moieties, a novel structural entity...
October 7, 2016: Journal of Biological Chemistry
Sonia Fernández-Menéndez, María L Fernández-Sánchez, Héctor González-Iglesias, Belén Fernández-Colomer, José López-Sastre, Alfredo Sanz-Medel
PURPOSE: In this work, the absorption and/or bioavailability of iron from two chemical species, (57)Fe-Lf (apo-lactoferrin) complex and (57)FeSO4 at low and high dose, and in Lf excess were investigated in lactating wistar rats. METHODS: The methodology used is based on the use of stable isotopes in combination with the approach "isotope pattern deconvolution" and ICP-MS for detection. This approach provides quantitative information about exogenous ((57)Fe) and endogenous iron ((nat)Fe) distribution in fluids and tissues in the iron-supplemented rat groups...
October 18, 2016: European Journal of Nutrition
Emilio Yángüez, Alicia García-Culebras, Aldo Frau, Catalina Llompart, Klaus-Peter Knobeloch, Sylvia Gutierrez-Erlandsson, Adolfo García-Sastre, Mariano Esteban, Amelia Nieto, Susana Guerra
[This corrects the article DOI: 10.1371/journal.ppat.1003632.].
October 2016: PLoS Pathogens
J Feliu, I Díez de Corcuera, J L Manzano, M Valladares-Ayerbes, J Alcaide, T García García, R Vera, J Sastre
PURPOSE: In the VELOUR study, aflibercept + FOLFIRI regimen resulted in improved survival in metastatic colorectal cancer (mCRC) patients who progressed after oxaliplatin. The use of aflibercept outside the clinical trial framework needs to be further assessed in terms of effectiveness and tolerability. METHODS: Early access to aflibercept through a named patient programme (NPP) was provided to mCRC patients receiving FOLFIRI as second-line treatment in Spain...
October 7, 2016: Clinical & Translational Oncology
Laura Mourino-Alvarez, Montserrat Baldan-Martin, Laura Gonzalez-Calero, Carlos Martinez-Laborde, Tamara Sastre-Oliva, Rafael Moreno-Luna, Luis F Lopez-Almodovar, Pedro L Sanchez, Francisco Fernandez-Aviles, Fernando Vivanco, Luis R Padial, Finn Akerstrom, Gloria Alvarez-Llamas, Fernando de la Cuesta, María G Barderas
BACKGROUND: The most common valve diseases are calcific aortic stenosis (AS) and aortic regurgitation (AR). The former is characterized by thickening of valve leaflets followed by progressive calcification, which produces progressive aortic valve (AV) narrowing, increased pressure afterload on the left ventricle (LV) and subsequent LV hypertrophy. On the other hand, AR is due to malcoaptation of the valve leaflets with resultant diastolic reflux of blood from aorta back to the LV producing volume and pressure overload and progressive LV dilatation...
September 26, 2016: International Journal of Cardiology
Nathalie Zink-Lorre, Enrique Font-Sanchis, Ángela Sastre-Santos, Fernando Fernández-Lázaro
Herein, we report a simple fluoride-mediated reaction for the direct mono- and dialkylthio-functionalization of unsubstituted perylenediimides (PDIs) under very mild conditions. The aromatic substitution reaction offers the possibility to introduce primary, secondary and, even, tertiary alkanethiols either on the 1- or on the 1,6-bay positions of unsubstituted PDIs. 1,6-DialkylthioPDIs show that absorption and fluorescence spectra shifted to the red when compared with the unsubstituted PDI, with Stokes shifts around 70-80 nm...
October 4, 2016: Organic & Biomolecular Chemistry
Cristina Díaz Del Arco, J Ángel Díaz Pérez, Luis Ortega Medina, Javier Sastre Valera, M Jesús Fernández Aceñero
Neuroendocrine pancreatic tumors (PanNETs) are graded on the basis of their proliferative activity. Cytological samples are commonly the only samples available, but the determination of Ki-67 in cytology and its reliability as a measure of tumor mitotic activity is not well settled. We have retrospectively reviewed all the cases of FNA under EUS control of PanNETs in a 10-year period (2006-2016) in the Hospital Clínico San Carlos (Madrid). We identified 10 PanNET cases with histological correlation. Median age was 49...
September 29, 2016: Endocrine Pathology
Joaquín Sastre, Marina Sastre-Ibañez
PURPOSE OF REVIEW: To describe recent insights into how molecular diagnosis can improve indication and selection of suitable allergens for specific immunotherapy and increase the safety of this therapy. RECENT FINDINGS: As specific allergen immunotherapy targets specific allergens, identification of the disease-eliciting allergen is a prerequisite for accurate prescription of treatment. In areas of complex sensitization to aeroallergens or in cases of hymenoptera venom allergy, the use of molecular diagnosis has demonstrated that it may lead to a change in indication and selection of allergens for immunotherapy in a large proportion of patients when compared with diagnosis based on skin prick testing and/or specific IgE determination with commercial extracts...
September 28, 2016: Current Opinion in Allergy and Clinical Immunology
M Moreno-Sastre, M Pastor, A Esquisabel, E Sans, M Viñas, D Bachiller, J L Pedraz
In the last decades, the encapsulation of antibiotics into nanoparticulate carriers has gained increasing attention for the treatment of infectious diseases. Sodium colistimethate-loaded solid lipid nanoparticles (Colist-SLNs) and nanostructured lipid carriers (Colist-NLCs) were designed aiming to treat the pulmonary infection associated to cystic fibrosis patients. The nanoparticles were freeze-dried using trehalose as cryoprotectant. The stability of both nanoparticles was analysed over one year according to the International Conference of Harmonisation (ICH) guidelines by determining the minimum inhibitory concentration (MIC) against clinically isolated Pseudomonas aeruginosa strains and by studying their physico-chemical characteristics...
October 14, 2016: Journal of Microencapsulation
Juan Carlos Fernández-Domínguez, Albert Sesé-Abad, Jose Miguel Morales-Asencio, Pedro Sastre-Fullana, Sandra Pol-Castañeda, Joan Ernest de Pedro-Gómez
OBJECTIVE: To develop a tool for measuring evidence-based practice (EBP) and to evaluate its content validity by Delphi technique. A five-factor/dimensions latent structure for the EBP construct was defined a priori and operationalized. DESIGN: Online Delphi technique. SETTING AND PARTICIPANTS: A group of 32 national EBP experts from different health professions. INTERVENTION: The experts rated the initial questionnaire items according to adequacy and relevance criteria using four-point Likert scales and including open fields for suggestions, with basic and supplementary criteria consensus established a priori...
September 21, 2016: International Journal for Quality in Health Care
Étori Aguiar Moreira, Anna Weber, Hardin Bolte, Larissa Kolesnikova, Sebastian Giese, Seema Lakdawala, Martin Beer, Gert Zimmer, Adolfo García-Sastre, Martin Schwemmle, Mindaugas Juozapaitis
Packaging of the eight genomic RNA segments of influenza A viruses (IAV) into viral particles is coordinated by segment-specific packaging sequences. How the packaging signals regulate the specific incorporation of each RNA segment into virions and whether other viral or host factors are involved in this process is unknown. Here, we show that distinct amino acids of the viral nucleoprotein (NP) are required for packaging of specific RNA segments. This was determined by studying the NP of a bat influenza A-like virus, HL17NL10, in the context of a conventional IAV (SC35M)...
September 21, 2016: Nature Communications
Leire Iparraguirre, Angela Gutierrez-Camino, Maitane Umerez, Idoia Martin-Guerrero, Itziar Astigarraga, Aurora Navajas, Ana Sastre, Nagore Garcia de Andoin, Africa Garcia-Orad
OBJECTIVES: Methotrexate (MTX), the key drug in childhood B-cell acute lymphoblastic leukemia (B-ALL) therapy, often causes toxicity. An association between genetic variants in MTX transport genes and toxicity has been found. It is known that these transporters are regulated by microRNAs (miRNAs), and miRNA single nucleotide polymorphisms (SNPs) interfere with miRNA levels or function. With regard to B-cell ALL, we have previously found rs56103835 in miR-323b that targets ABCC4 associated with MTX plasma levels...
November 2016: Pharmacogenetics and Genomics
Jose María González-Santos, María Elena Arnáiz-García, María José Dalmau-Sorlí, Jose Alfonso Sastre-Rincón, Jesús Hernández-Hernández, María Elena Pérez-Losada, Víctor Sagredo-Meneses, Javier López-Rodríguez
A patient who underwent previous implantation of a mitral valve replacement with a Björk-Shiley Delrin (BSD) mitral valve prosthesis during infancy was admitted to our institution 43 years later after an episode of syncope and cardiac arrest. Under extreme hemodynamic instability, a mitral valve prosthetic dysfunction causing massive mitral regurgitation was identified. The patient underwent an emergent cardiac operation, and a complete disc fracture with partial disc migration was found. Exceptional cases of mechanical prosthetic heart valve fracture exist...
October 2016: Annals of Thoracic Surgery
Javier Santabárbara, Patricia Gracia-García, Guillermo Pírez, Raúl López-Antón, Concepcion De La Cámara, Tirso Ventura, Marina Pérez-Sastre, Elena Lobo, Pedro Saz, Guillermo Marcos, Antonio Lobo
OBJECTIVE: To explore the possibility that the mortality risk of mild cognitive impairment (MCI) as diagnosed using Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition (DSM-5) criteria (DSM-5-MCI) will be higher than using Petersen's criteria (P-MCI) and to report the population-attributable fraction (PAF) of mortality due to MCI. METHODS: A representative community sample of 4,803 individuals aged 55 or more years was interviewed and then followed for 17 years...
November 2016: American Journal of Geriatric Psychiatry
Ana Hidalgo-Sastre, Roxanne L Brodylo, Clara Lubeseder-Martellato, Bence Sipos, Katja Steiger, Marcel Lee, Guido von Figura, Barbara Grünwald, Suyang Zhong, Marija Trajkovic-Arsic, Florian Neff, Roland M Schmid, Jens T Siveke
Perturbation of the pancreatic acinar cell state can lead to acinar-to-ductal metaplasia (ADM), which is a precursor lesion to the development of pancreatic ductal adenocarcinoma (PDAC). In the pancreas, Notch signaling is active both during development and in adult cellular differentiation processes. Hes1, a key downstream target of the Notch signaling pathway, is expressed in the centroacinar compartment of the adult pancreas as well as in both preneoplastic and malignant lesions. In this study, we used a murine genetic in vivo approach to ablate Hes1 in pancreatic progenitor cells (Ptf1a(+/Cre); Hes1(fl/fl))...
September 14, 2016: American Journal of Pathology
P Sastre, C Gallardo, A Monedero, T Ruiz, M Arias, A Sanz, P Rueda
BACKGROUND: African swine fever (ASF) is a viral infectious disease of domestic and wild suids of all breeds and ages, causing a wide range of hemorrhagic syndromes and frequently characterized by high mortality. The disease is endemic in Sub-Saharan Africa and Sardinia. Since 2007, it has also been present in different countries of Eastern Europe, where control measures have not been effective so far. The continued spread poses a serious threat to the swine industry worldwide. In the absence of vaccine, early detection of infected animals is of paramount importance for control of the outbreak, to prevent the transmission of the virus to healthy animals and subsequent spreading of the disease...
2016: BMC Veterinary Research
Javier Coloma, Rinku Jain, Kanagalaghatta R Rajashankar, Adolfo García-Sastre, Aneel K Aggarwal
The Zika virus (ZIKV) poses a major public health emergency. To aid in the development of antivirals, we present two high-resolution crystal structures of the ZIKV NS5 methyltransferase: one bound to S-adenosylmethionine (SAM) and the other bound to SAM and 7-methyl guanosine diphosphate (7-MeGpp). We identify features of ZIKV NS5 methyltransferase that lend to structure-based antiviral drug discovery. Specifically, SAM analogs with functionalities on the Cβ atom of the methionine portion of the molecules that occupy the RNA binding tunnel may provide better specificity relative to human RNA methyltransferases...
September 20, 2016: Cell Reports
Maude Guillot-Delost, Lia Guilleré, Frédérique Berger, Aurore Ventre, Paula Michea, Philémon Sirven, Lucia Pattarini, Alix Scholer-Dahirel, Fatima-Zahra Kebir, Michel Huerre, Olfa Chouchane-Mlik, Emmanuelle Lappartient, José Rodriguez, Thomas Jouffroy, Jerzy Klijanienko, André Nicolas, Xavier Sastre-Garau, Sofia Honorio, Véronique Mosseri, Nelly Le Peltier, Marie-Paule Sablin, Christophe Le Tourneau, Éric Tartour, Cécile Badoual, Vassili Soumelis
Thymic stromal lymphopoietin (TSLP) is an interleukin (IL)-7-like cytokine expressed by epithelial cells during allergic inflammation, and activating dendritic cells (DC). Its expression and functional role in cancer remain controversial. We conducted retrospective (n = 89), and prospective studies including patients with untreated primary head and neck squamous cell carcinoma (HNSCC). We found that TSLP was overexpressed by HNSCC tumor cells, and associated with a highly differentiated status. However, no significant difference in overall and recurrence-free survival was found between patients bearing a tumor with high and low TSLP levels, respectively...
July 2016: Oncoimmunology
Christine Sedlik, Adèle Heitzmann, Sophie Viel, Rafik Ait Sarkouh, Cornélie Batisse, Frédéric Schmidt, Philippe De La Rochere, Nathalie Amzallag, Eduardo Osinaga, Pablo Oppezzo, Otto Pritsch, Xavier Sastre-Garau, Pascale Hubert, Sebastian Amigorena, Eliane Piaggio
Antibody-drug conjugates (ADC), combining the specificity of tumor recognition by monoclonal antibodies (mAb) and the powerful cytotoxicity of anticancer drugs, are currently under growing interest and development. Here, we studied the potential of Chi-Tn, a mAb directed to a glyco-peptidic tumor-associated antigen, to be used as an ADC for cancer treatment. First, we demonstrated that Chi-Tn specifically targeted tumor cells in vivo. Also, using flow cytometry and deconvolution microscopy, we showed that the Chi-Tn mAb is rapidly internalized - condition necessary to ensure the delivery of conjugated cytotoxic drugs in an active form, and targeted to early and recycling endosomes...
July 2016: Oncoimmunology
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