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https://www.readbyqxmd.com/read/28101294/erratum-to-risk-and-safety-requirements-for-diagnostic-and-therapeutic-procedures-in-allergology-world-allergy-organization-statement
#1
Marek L Kowalski, Ignacio Ansotegui, Werner Aberer, Mona Al-Ahmad, Mubeccel Akdis, Barbara K Ballmer-Weber, Kirsten Beyer, Miguel Blanca, Simon Brown, Chaweewan Bunnag, Arnaldo Capriles Hulett, Mariana Castells, Hiok Hee Chng, Frederic De Blay, Motohiro Ebisawa, Stanley Fineman, David B K Golden, Tari Haahtela, Michael Kaliner, Connie Katelaris, Bee Wah Lee, Joanna Makowska, Ulrich Muller, Joaquim Mullol, John Oppenheimer, Hae-Sim Park, James Parkerson, Giovanni Passalacqua, Ruby Pawankar, Harald Renz, Franziska Rueff, Mario Sanchez-Borges, Joaquin Sastre, Glenis Scadding, Scott Sicherer, Pongsakorn Tantilipikorn, James Tracy, Vera van Kampen, Barbara Bohle, G Walter Canonica, Luis Caraballo, Maximiliano Gomez, Komei Ito, Erika Jensen-Jarolim, Mark Larche, Giovanni Melioli, Lars K Poulsen, Rudolf Valenta, Torsten Zuberbier
[This corrects the article DOI: 10.1186/s40413-016-0122-3.].
2017: World Allergy Organization Journal
https://www.readbyqxmd.com/read/28096299/exosomes-from-eosinophils-autoregulate-and-promote-eosinophil-functions
#2
José Antonio Cañas, Beatriz Sastre, Carla Mazzeo, Mar Fernández-Nieto, José Manuel Rodrigo-Muñoz, Andrés González-Guerra, Manuel Izquierdo, Pilar Barranco, Santiago Quirce, Joaquín Sastre, Victoria Del Pozo
Eosinophils are able to secrete exosomes that have an undefined role in asthma pathogenesis. We hypothesized that exosomes released by eosinophils autoregulate and promote eosinophil function. Eosinophils of patients with asthma (n = 58) and healthy volunteers (n = 16) were purified from peripheral blood, and exosomes were isolated and quantified from eosinophils of the asthmatic and healthy populations. Apoptosis, adhesion, adhesion molecules expression, and migration assays were performed with eosinophils in the presence or absence of exosomes from healthy and asthmatic individuals...
January 17, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28089234/oncogenic-kras-induced-increase-in-fluid-phase-endocytosis-is-dependent-on-n-wasp-and-is-required-for-the-formation-of-pancreatic-preneoplastic-lesions
#3
Clara Lubeseder-Martellato, Katharina Alexandrow, Ana Hidalgo-Sastre, Irina Heid, Sophie Luise Boos, Thomas Briel, Roland M Schmid, Jens T Siveke
Fluid-phase endocytosis is a homeostatic process with an unknown role in tumor initiation. The driver mutation in pancreatic ductal adenocarcinoma (PDAC) is constitutively active KRas(G12D), which induces neoplastic transformation of acinar cells through acinar-to-ductal metaplasia (ADM). We have previously shown that KRas(G12D)-induced ADM is dependent on RAC1 and EGF receptor (EGFR) by a not fully clarified mechanism. Using three-dimensional mouse and human acinar tissue cultures and genetically engineered mouse models, we provide evidence that (i) KRas(G12D) leads to EGFR-dependent sustained fluid-phase endocytosis (FPE) during acinar metaplasia; (ii) variations in plasma membrane tension increase FPE and lead to ADM in vitro independently of EGFR; and (iii) that RAC1 regulates ADM formation partially through actin-dependent regulation of FPE...
December 24, 2016: EBioMedicine
https://www.readbyqxmd.com/read/28089097/a-systematic-approach-for-the-chromatographic-fractionation-and-purification-of-steroid-saponins-in-commercial-extracts-of-yucca-schidigera-roezl
#4
F Sastre, F Ferreira, F Pedreschi
Yucca schidigera Roezl. (yucca) is one of the major industrial sources of steroid saponins used as animal and human food additives. This work describes a new, systematic and reproducible three-step method by medium and high-pressure liquid chromatography (under RP, NP and RP conditions), for the isolation and purification of three groups of saponins, which were further purified in sub-fractions, and finally into twelve individual steroid saponins previously reported in Y. schidigera. In accordance to the increasing applications of yucca extracts, further analytical, biological and physicochemical studies are still required...
November 23, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28067239/corrigendum-influenza-virus-mrna-trafficking-through-host-nuclear-speckles
#5
Amir Mor, Alexander White, Ke Zhang, Matthew Thompson, Matthew Esparza, Raquel Muñoz-Moreno, Kazunori Koide, Kristen W Lynch, Adolfo García-Sastre, Beatriz M A Fontoura
No abstract text is available yet for this article.
January 9, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/28062361/redox-signaling-in-the-gastrointestinal-tract
#6
REVIEW
Salvador Pérez, Raquel Taléns-Visconti, Sergio Rius-Pérez, Isabela Finamor, Juan Sastre
Redox signaling regulates physiological self-renewal, proliferation, migration and differentiation in gastrointestinal epithelium by modulating Wnt/β-catenin and Notch signaling pathways mainly through NADPH oxidases (NOXs). In the intestine, intracellular and extracellular thiol redox status modulates the proliferative potential of epithelial cells. Furthermore, commensal bacteria contribute to intestine epithelial homeostasis through NOX1- and dual oxidase 2-derived reactive oxygen species (ROS). The loss of redox homeostasis is involved in the pathogenesis and development of a wide diversity of gastrointestinal disorders, such as Barrett's esophagus, esophageal adenocarcinoma, peptic ulcer, gastric cancer, ischemic intestinal injury, celiac disease, inflammatory bowel disease and colorectal cancer...
January 3, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28059146/influenza-virus-exploits-tunneling-nanotubes-for-cell-to-cell-spread
#7
Amrita Kumar, Jin Hyang Kim, Priya Ranjan, Maureen G Metcalfe, Weiping Cao, Margarita Mishina, Shivaprakash Gangappa, Zhu Guo, Edward S Boyden, Sherif Zaki, Ian York, Adolfo García-Sastre, Michael Shaw, Suryaprakash Sambhara
Tunneling nanotubes (TNTs) represent a novel route of intercellular communication. While previous work has shown that TNTs facilitate the exchange of viral or prion proteins from infected to naïve cells, it is not clear whether the viral genome is also transferred via this mechanism and further, whether transfer via this route can result in productive replication of the infectious agents in the recipient cell. Here we present evidence that lung epithelial cells are connected by TNTs, and in spite of the presence of neutralizing antibodies and an antiviral agent, Oseltamivir, influenza virus can exploit these networks to transfer viral proteins and genome from the infected to naïve cell, resulting in productive viral replication in the naïve cells...
January 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28057438/oncogenic-kras-induced-increase-in-fluid-phase-endocytosis-is-dependent-on-n-wasp-and-is-required-for-the-formation-of-pancreatic-preneoplastic-lesions
#8
Clara Lubeseder-Martellato, Katharina Alexandrow, Ana Hidalgo-Sastre, Irina Heid, Sophie Luise Boos, Thomas Briel, Roland M Schmid, Jens T Siveke
Fluid-phase endocytosis is a homeostatic process with an unknown role in tumor initiation. The driver mutation in pancreatic ductal adenocarcinoma (PDAC) is constitutively active KRas(G12D), which induces neoplastic transformation of acinar cells through acinar-to-ductal metaplasia (ADM). We have previously shown that KRas(G12D)-induced ADM is dependent on RAC1 and EGF receptor (EGFR) by a not fully clarified mechanism. Using three-dimensional mouse and human acinar tissue cultures and genetically engineered mouse models, we provide evidence that (i) KRas(G12D) leads to EGFR-dependent sustained fluid-phase endocytosis (FPE) during acinar metaplasia; (ii) variations in plasma membrane tension increase FPE and lead to ADM in vitro independently of EGFR; and (iii) that RAC1 regulates ADM formation partially through actin-dependent regulation of FPE...
February 2017: EBioMedicine
https://www.readbyqxmd.com/read/28054016/data-set-on-a-study-of-gene-expression-in-peripheral-samples-to-identify-biomarkers-of-severity-of-allergic-and-nonallergic-asthma
#9
Selene Baos, David Calzada, Lucía Cremades, Joaquín Sastre, Joaquín Quiralte, Fernando Florido, Carlos Lahoz, Blanca Cárdaba
This article contains information related to the research article entitled "Biomarkers associated with disease severity in allergic and nonallergic asthma" (S. Baos, D. Calzada, L. Cremades, J. Sastre, J. Quiralte, F. Florido, C. Lahoz, B. Cárdaba, In press). Specifically, the clinical criteria stablished for selecting the study population (n=104 subjects) are described. Moreover, this article describes the criteria for selecting the 94 genes to be analyzed in PBMCs (peripheral blood mononuclear cells), it is provided a description of these genes and a Table with the genes most differentially expressed by clinical phenotypes and, finally it is detailed the experimental methodology followed for studying the protein expression of MSR1 (macrophage scavenger receptor 1), one of the genes evaluated in the research...
February 2017: Data in Brief
https://www.readbyqxmd.com/read/28038922/oct-angiography-in-retinal-arterial-macroaneurysm
#10
P Gili Manzanaro, H Santiago-Balsera, I Velasco Sastre
No abstract text is available yet for this article.
December 27, 2016: Archivos de la Sociedad Española de Oftalmología
https://www.readbyqxmd.com/read/28032871/debate-multidisciplinar-de-expertos-asma-no-controlada-causas-consecuencias-y-posibles-soluciones
#11
V Plaza Moral, T Carrillo, J Delgado, L M Entrenas, E Martinez Moragón, J M Olaguibel, L Pérez de Llano, M Perpiñá, J Sastre, A Valero, S Quirce
  Fundamento. Numerosos estudios constatan que aproximadamente la mitad de la población asmática no está bien controlada. El objetivo de este trabajo fue analizar entre expertos las causas, consecuencias y las posibles soluciones del asma no controlada (ANC).  Material y métodos. Debate entre expertos en asma de las especialidades de Neumología, Alergología y Atención Primaria, estructurado en tres fases 1) Cuestionario para pulsar la opinión de los participantes en los diferentes elementos implicados del ANC; 2) Reunión presencial de los expertos, en la que se discutieron los resultados de la encuesta y se presentaron y debatieron ponencias sobre el diagnóstico, tratamiento y seguimiento del ANC; 3) Formulación de 83 ítems que fueron sometidos a consenso entre todos los participantes mediante el método Delphi...
November 21, 2016: Anales del Sistema Sanitario de Navarra
https://www.readbyqxmd.com/read/28029407/management-of-chronic-spontaneous-urticaria-in-routine-clinical-practice-a-delphi-method-questionnaire-among-specialists-to-test-agreement-with-current-european-guidelines-statements
#12
A Giménez-Arnau, M Ferrer, J Bartra, I Jáuregui, M Labrador-Horrillo, J Ortiz de Frutos, J F Silvestre, J Sastre, M Velasco, A Valero
BACKGROUND: Chronic spontaneous urticaria (CSU) is a frequent clinical entity that often presents a diagnostic and therapeutic challenge. OBJECTIVE: To explore the degree of agreement that exists among the experts caring for patients with CSU diagnosis, evaluation, and management. METHODS: An online survey was conducted to explore the opinions of experts in CSU, address controversial issues, and provide recommendations regarding its definition, natural history, diagnosis, and treatment...
October 28, 2016: Allergologia et Immunopathologia
https://www.readbyqxmd.com/read/28019644/allergic-rhinitis-severity-can-be-assessed-using-a-visual-analogue-scale-in-mild-moderate-and-severe
#13
A Del Cuvillo, V Santos, J Montoro, J Bartra, I Davila, M Ferrer, I Jauregui, J Sastre, J Mullol, A Valero
BACKGROUND: Allergic rhinitis is a global healthcare problem due to its high prevalence, impact on individuals and socioeconomic burden for the nations. Allergic rhinitis severity evaluation is the key to a correct treatment, prevention of comorbidities and improving the quality of life of patients. This evaluation should be made with a simple, easy, fast but accurate and reliable methodology, both in a primary care and specialist setting. The visual analogue scale (VAS) meets all requirements to be the ideal tool to assess allergic rhinitis severity and has already been validated by using a single cut-off point, but this classification in two degrees of severity suffer from not allocating the patients uniformly and from giving a blind interval to classify the patients when the score is between 5 to 6 cm...
December 26, 2016: Rhinology
https://www.readbyqxmd.com/read/28011367/biomarkers-associated-with-disease-severity-in-allergic-and-nonallergic-asthma
#14
Selene Baos, David Calzada, Lucía Cremades, Joaquín Sastre, Joaquín Quiralte, Fernando Florido, Carlos Lahoz, Blanca Cárdaba
Asthma is a complex, chronic respiratory disease with a wide clinical spectrum. Use of high-throughput technologies has generated a great deal of data that require validation. In this work the objective was to validate molecular biomarkers related to asthmatic disease types in peripheral blood samples and define their relationship with disease severity. With this purpose, ninety-four previously described genes were analyzed by qRT-PCR in 30 healthy control (HC) subjects, 30 patients with nonallergic asthma (NA), 30 with allergic asthma (AA), and 14 patients with allergy (rhinitis) but without asthma (AR)...
December 20, 2016: Molecular Immunology
https://www.readbyqxmd.com/read/28002670/chemoenzymatic-approach-for-the-preparation-of-asymmetric-bi-tri-and-tetra-antennary-n-glycans-from-a-common-precursor
#15
Ivan A Gagarinov, Tiehai Li, Javier Sastre Toraño, Tomislav Caval, Apoorva D Srivastava, John A W Kruijtzer, Albert J R Heck, Geert-Jan Boons
Progress in glycoscience is hampered by a lack of well-defined complex oligosaccharide standards that are needed to fabricate the next generation of microarrays, to develop analytical protocols to determine exact structures of isolated glycans, and to elucidate pathways of glycan biosynthesis. We describe here a chemoenzymatic methodology that makes it possible, for the first time, to prepare any bi-, tri-, and tetra-antennary asymmetric N-glycan from a single precursor. It is based on the chemical synthesis of a tetra-antennary glycan that has N-acetylglucosamine (GlcNAc), N-acetyllactosamine (LacNAc), and unnatural Galα(1,4)-GlcNAc and Manβ(1,4)-GlcNAc appendages...
January 18, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/27996959/reply-to-liccardi-et-al
#16
LETTER
J Sastre
No abstract text is available yet for this article.
2016: Journal of Investigational Allergology & Clinical Immunology
https://www.readbyqxmd.com/read/27983954/a-lateral-flow-assay-lfa-for-the-rapid-detection-of-extraparenchymal-neurocysticercosis-using-cerebrospinal-fluid
#17
Agnes Fleury, Patricia Sastre, Edda Sciutto, Silvia Correia, Alejandro Monedero, Andrea Toledo, Maricela Hernandez, Leslie J S Harrison, R Michael E Parkhouse
A lateral flow assay (LFA) for the diagnosis and monitoring of extraparenchymal neurocysticercosis, has been developed. The assay is based on the use of the monoclonal antibody HP10, and when applied to cerebrospinal fluid, correctly identified 34 cases of active extraparenchymal neurocysticercosis, but was negative with 26 samples from treated and cured neurocysticercosis patients and with 20 samples from unrelated neurological diseases. There was complete agreement between the HP10 Ag-ELISA results and the HP10-LFA...
October 27, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27955849/-gallbladder-volvulus-diagnostic-and-surgical-challenges
#18
Pedro Abadía-Barnó, Magdalena Coll-Sastre, Carmen Picón-Serrano, Alfonso Sanjuanbenito-Dehesa, Jacobo Cabañas-Montero
BACKGROUND: The gallbladder volvulus is defined as the rotation of the gallbladder on its mesentery along the axis of the cystic duct and cystic artery. It is an extremely rare surgical disease and definitive diagnosis is usually made during surgery. CASE REPORT: A 78 year old woman presented with upper right quadrant abdominal pain, with no comorbidities and no other accompanying symptoms. Analysis revealed haemodynamic instability and leukocytosis. Computed tomography of abdomen showed an acute cholecystitis...
December 9, 2016: Cirugia y Cirujanos
https://www.readbyqxmd.com/read/27939406/safety-of-immunotherapy-with-glutaraldehyde-modified-allergen-extracts-in-children-and-adults
#19
M Guzmán-Fulgencio, R Caballero, B Lara, M Mena, M Tejera, A Sastre, J-L Subiza, E Fernández-Caldas, M Casanovas
BACKGROUND: Glutaraldehyde-modified natural allergen extracts show significant reduction in the IgE-binding capacity and proteolytic activity. This allows the administration of higher doses in a shorter period of time, and to mix different allergen extracts. OBJECTIVE: Evaluate the safety of different concentrations and mixtures of glutaraldehyde-modified allergen extracts in a large group of paediatric and adult patients undergoing specific immunotherapy treatment...
December 6, 2016: Allergologia et Immunopathologia
https://www.readbyqxmd.com/read/27917295/circulating-human-papillomavirus-dna-detected-using-droplet-digital-pcr-in-the-serum-of-patients-diagnosed-with-early-stage-human-papillomavirus-associated-invasive-carcinoma
#20
Emmanuelle Jeannot, Véronique Becette, Maura Campitelli, Marie-Ange Calméjane, Emmanuelle Lappartient, Evelyne Ruff, Stéphanie Saada, Allyson Holmes, Dominique Bellet, Xavier Sastre-Garau
Specific human papillomavirus genotypes are associated with most ano-genital carcinomas and a large subset of oro-pharyngeal carcinomas. Human papillomavirus DNA is thus a tumour marker that can be detected in the blood of patients for clinical monitoring. However, data concerning circulating human papillomavirus DNA in cervical cancer patients has provided little clinical value, due to insufficient sensitivity of the assays used for the detection of small sized tumours. Here we took advantage of the sensitive droplet digital PCR method to identify circulating human papillomavirus DNA in patients with human papillomavirus-associated carcinomas...
October 2016: Journal of Pathology. Clinical Research
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