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Utpal Bose, Catharine A Ortori, Sarir Sarmad, David A Barrett, Amitha K Hewavitharana, Mark P Hodson, John A Fuerst, P Nicholas Shaw
The structures of acyl homoserine lactone (AHL) compounds and their quantification was accomplished using an integrated liquid chromatography-mass spectrometry approach. The precursor and product ions, along with retention times of peaks, were searched against an in-house database of AHLs and structures confirmed by accurate mass and by comparison with authentic AHL standards. The two compounds, N-(3-oxodecanoyl)-L-homoserine lactone and N-(3-oxododecanoyl)-L-homoserine lactone were characterised and quantified in Salinispora sp...
January 12, 2017: FEMS Microbiology Letters
Jonathan Ström, Johan Tham, Fredrik Månsson, Jonas Ahl, Tor C Savidge, Sara M Dann, Fredrik Resman
OBJECTIVE: Recently, metabolomics studies have suggested that the neurotransmitter γ-amino butyric acid (GABA) may modulate C. difficile infection (CDI) pathogenesis. In the present study, we investigated the association between GABA-modulating pharmaceuticals and CDI development. METHODS: In July-December 2013, we performed a matched, retrospective case-control study in Skåne county, Sweden, to assess the association between the use of GABA-modulators (defined as regular use of at least one of the following: zolpidem, zopiclone, benzodiazepines, gabapentin, pregabalin or baclofen) and CDI...
2017: PloS One
Nripendra N Biswas, Tsz Tin Yu, Önder Kimyon, Shashidhar Nizalapur, Christopher R Gardner, Mike Manefield, Renate Griffith, David StC Black, Naresh Kumar
Bacteria communicate with one another and regulate their pathogenicity through a phenomenon known as quorum sensing (QS). When the bacterial colony reaches a threshold density, the QS system induces the production of virulence factors and the formation of biofilms, a powerful defence system against the host's immune responses. The glucosamine monomer has been shown to disrupt the bacterial QS system by inhibiting autoinducer (AI) signalling molecules such as the acyl-homoserine lactones (AHLs). In this study, the synthesis of acetoxy-glucosamides 8, hydroxy-glucosamides 9 and 3-oxo-glucosamides 12 was performed via the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC·HCl) and N,N'-dicyclohexylcarbodiimide (DCC) coupling methods...
December 21, 2016: Bioorganic & Medicinal Chemistry
Michelle C Janelsins, Charles E Heckler, Luke J Peppone, Charles Kamen, Karen M Mustian, Supriya G Mohile, Allison Magnuson, Ian R Kleckner, Joseph J Guido, Kelley L Young, Alison K Conlin, Lora R Weiselberg, Jerry W Mitchell, Christine A Ambrosone, Tim A Ahles, Gary R Morrow
Purpose Cancer-related cognitive impairment is an important problem for patients with breast cancer, yet its trajectory is not fully understood. Some previous cancer-related cognitive impairment research is limited by heterogeneous populations, small samples, lack of prechemotherapy and longitudinal assessments, use of normative data, and lack of generalizability. We addressed these limitations in a large prospective, longitudinal, nationwide study. Patients and Methods Patients with breast cancer from community oncology clinics and age-matched noncancer controls completed the Functional Assessment of Cancer Therapy-Cognitive Function (FACT-Cog) at prechemotherapy and postchemotherapy and at a 6-month follow-up as an a priori exploratory aim...
December 28, 2016: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
Shuxin Zhou, An Zhang, Hongping Yin, Weihua Chu
Quorum sensing (QS) is a cell density dependent process that enables bacteria to communicate with each other based on the production, secretion and sensing of the auto-inducer molecules and then subsequently regulate virulence associated gene expression. Interrupting quorum sensing may represent a novel alternative approach to combat bacterial pathogen. Several bacteria can produce quorum quenching (QQ) enzymes. However, the role of QQ bacteria in shaping the microbiota and the level of N-acyl-homoserine lactones (AHLs, a prevalent type of QS molecules) producing bacteria remains largely unknown...
2016: Frontiers in Cellular and Infection Microbiology
Shengcai Han, Dan Li, Eva Trost, Klaus F Mayer, A Corina Vlot, Werner Heller, Michael Schmid, Anton Hartmann, Michael Rothballer
Quorum sensing auto-inducers of the N-acyl homoserine lactone (AHL) type produced by Gram-negative bacteria have different effects on plants including stimulation on root growth and/or priming or acquirement of systemic resistance in plants. In this communication the influence of AHL production of the plant growth promoting endophytic rhizosphere bacterium Acidovorax radicis N35 on barley seedlings was investigated. A. radicis N35 produces 3-hydroxy-C10-homoserine lactone (3-OH-C10-HSL) as the major AHL compound...
2016: Frontiers in Plant Science
Rebecka Ahl, Herb A Phelan, Sinan Dogan, Yang Cao, Allyson C Cook, Shahin Mohseni
BACKGROUND: The Geriatric Trauma Outcome Score, GTOS (= [age] + [Injury Severity Score (ISS)x2.5] + 22 [if packed red blood cells (PRBC) transfused ≤24hrs of admission]), was developed and validated as a prognostic indicator for in-hospital mortality in elderly trauma patients. However, GTOS neither provides information regarding post-discharge outcomes, nor discriminates between patients dying with and without care restrictions. Isolating the latter, GTOS prediction performance was examined during admission and 1-year post-discharge in a mature European trauma registry...
December 22, 2016: Journal of the American College of Surgeons
Jinfeng Wang, Lili Ding, Kan Li, Wilhelm Schmieder, Jinju Geng, Ke Xu, Yan Zhang, Hongqiang Ren
N-Acylated-l-homoserine lactones (AHLs) play a vital role in Gram-negative bacteria communication by promoting the formation of extracellular polymeric substances (EPS) and biofilms. However, the low concentration of these AHL signals makes the process difficult to understand. A robust and sensitive pretreatment method for AHL detection was developed in this work. Compared with eight different solid-phase extraction (SPE) columns and three various solid extraction method, we found that the UE (ultrasonic extraction) and an Oasis hydrophilic-lipophilic-balanced (HLB) sorbent in column format combined with ultra-performance liquid chromatography linked to tandem mass spectrometry (UPLC-MS/MS) can be successfully used for systematic pretreating moving bed biofilm reactor (MBBR) biological samples to extract AHLs and determine concentration of AHLs in wastewater treatment biofilms...
January 15, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Heather M Snyder, Tim Ahles, Stuart Calderwood, Maria C Carrillo, Honglei Chen, Chung-Chou H Chang, Suzanne Craft, Philip De Jager, Jane A Driver, Howard Fillit, David Knopman, Michael Lotze, Mary C Tierney, Suzana Petanceska, Andrew Saykin, Sudha Seshadri, Diana Shineman, Mary Ganguli
Recent population studies suggest an intriguing inverse relationship between several types of cancer and neurodegenerative diseases, including Alzheimer's disease. Understanding the intersection of the underlying biology for these two distinct families of diseases with one another may offer novel approaches to identify new therapeutic approaches and possible opportunities to repurpose existing drug candidates. The Alzheimer's Association and the Alzheimer's Drug Discovery Foundation convened a one-day workshop to delve into this discussion...
December 18, 2016: Alzheimer's & Dementia: the Journal of the Alzheimer's Association
Munmun Nandi, Carrie Selin, Gabriel Brawerman, W G Dilantha Fernando, Teresa R de Kievit
Pseudomonas chlororaphis PA23 is a biocontrol agent capable of protecting canola from stem rot disease caused by the fungus Sclerotinia sclerotiorum. The focus of the current study was to elucidate the role of the transcriptional regulator ANR in the biocontrol capabilities of this bacterium. An anr mutant was created, PA23anr, that was devoid antifungal activity. In other pseudomonads, ANR is essential for regulating HCN production. Characterization of PA23anr revealed that, in addition to HCN, ANR controls phenazine (PHZ), pyrrolnitrin (PRN), protease and autoinducer (AHL) signal molecule production...
December 2016: Microbiology
Zhi P Ma, Yong M Lao, Hui Jin, Guang H Lin, Zhong H Cai, Jin Zhou
Mangrove rhizosphere environment harbors diverse populations of microbes, and some evidence showed that rhizobacteria behavior was regulated by quorum sensing (QS). Investigating the diverse profiles of QS molecules in mangrove ecosystems may shed light on the bacterial roles and lead to a better understanding of the symbiotic interactions between plants and microbes. The aims of the current study focus on identifying AI-1 type QS signals, i.e., acyl homoserine lactones (AHLs), in Kandelia obovata rhizosphere environment...
2016: Frontiers in Microbiology
Shen Shen Kong, Rong Quan Zheng, Qi Peng Zhang
The genus Quasipaa (Family Dicroglossidae) is currently composed of 11 species distributed in China and Southeast Asia: Quasipaa acanthophora (Dubois & Ohler 2009), Q. boulengeri (Günther 1889), Q. courtoisi (Angel 1922), Q. delacouri (Angel 1928), Q. exilispinosa (Liu & Hu, 1975), Q. fasciculispina (Inger 1970), Q. jiulongensis (Huang & Liu, 1985), Q. shini (Ahl 1930), Q. spinosa (David 1875), Q. verrucospinosa (Bourret 1937), Q. yei (Chen, Qu & Jiang 2002) (Frost 2016). These species are morphologically similar, and their taxonomy is subject to controversy (Che et al...
December 4, 2016: Zootaxa
L I Ahl, H L Pedersen, Wgt Willats, N Rønsted, O M Grace
No abstract text is available yet for this article.
December 2016: Planta Medica
Simone Rankl, Benet Gunsé, Tina Sieper, Christoph Schmid, Charlotte Poschenrieder, Peter Schröder
While colonizing the rhizosphere, bacterial intra- and inter-specific communication is accomplished by N-Acyl-homoserine-lactones (AHLs) in a density-dependent manner. Moreover, plants are naturally exposed to AHLs and respond with tissue-specificity. In the present study, we investigated the influence of N-hexanoyl- (C6-HSL), N-octanoyl- (C8-HSL) and N-dodecanoyl-d/l-homoserine lactone (C12-HSL) on growth and root development in barley (Hordeum vulgare L.), and identified initial reactions in root cells after AHL exposures using physiological, staining, and electrophysiological methods...
December 2016: Plant Science: An International Journal of Experimental Plant Biology
Yong-Heng Huang, Jin-Shui Lin, Jin-Cheng Ma, Hai-Hong Wang
Pseudomonas aeruginosa is extremely resistant to triclosan. Previous studies have shown that P. aeruginosa encodes a triclosan-resistant enoyl-acyl-carrier protein reductase (ENR), FabV, and that deletion of fabV causes P. aeruginosa to be extremely sensitive to triclosan. In this report, we complemented a P. aeruginosa fabV deletion strain with several triclosan-resistant ENR encoding genes, including Vibrio cholerae fabV, Bacillus subtilis fabL and Enterococcus faecalis fabK. All complemented strains restored triclosan resistance to the level of the wild-type strain, which confirmed that triclosan-resistant ENR allows P...
2016: Frontiers in Microbiology
Richard J Hodes, Felipe Sierra, Steven N Austad, Elissa Epel, Gretchen N Neigh, Kristine M Erlandson, Marissa J Schafer, Nathan K LeBrasseur, Christopher Wiley, Judith Campisi, Mary E Sehl, Rosario Scalia, Satoru Eguchi, Balakuntalam S Kasinath, Jeffrey B Halter, Harvey Jay Cohen, Wendy Demark-Wahnefried, Tim A Ahles, Nir Barzilai, Arti Hurria, Peter W Hunt
It has long been known that aging, at both the cellular and organismal levels, contributes to the development and progression of the pathology of many chronic diseases. However, much less research has examined the inverse relationship-the contribution of chronic diseases and their treatments to the progression of aging-related phenotypes. Here, we discuss the impact of three chronic diseases (cancer, HIV/AIDS, and diabetes) and their treatments on aging, putative mechanisms by which these effects are mediated, and the open questions and future research directions required to understand the relationships between these diseases and aging...
December 2016: Annals of the New York Academy of Sciences
Shanshan Zhang, Ningning Liu, Weikang Liang, Qingxi Han, Weiwei Zhang, Chenghua Li
In the present study, the effects of an environmental friendly natural reagent coumarin, on the growth and potential virulence factors, as well as its ability to interfere the infection of Vibrio splendidus (Vs), were determined. Coumarin showed no effects on the maximal growth of Vs, and biofilm formation of Vs, while it significantly decreased protease activity and hemolytic activity by 43 and 80%, respectively. Correspondingly, coumarin exhibited an obviously protective effect, with a relative percent survival of 60% upon Apostichopus japonicus from infection by Vs...
December 9, 2016: Applied Microbiology and Biotechnology
Avinash Vellore Sunder, Putri Dwi Utari, Sureshkumar Ramasamy, Ronald van Merkerk, Wim Quax, Archana Pundle
Virulence pathways in gram-negative pathogenic bacteria are regulated by quorum sensing mechanisms, through the production and sensing of N-acylhomoserine lactone (AHL) signal molecules. Enzymatic degradation of AHLs leading to attenuation of virulence (quorum quenching) could pave the way for the development of new antibacterials. Penicillin V acylases (PVAs) belong to the Ntn hydrolase superfamily, together with AHL acylases. PVAs are exploited widely in the pharmaceutical industry, but their role in the natural physiology of their native microbes is not clearly understood...
December 8, 2016: Applied Microbiology and Biotechnology
Felipe Alves de Almeida, Natan de Jesus Pimentel-Filho, Lanna Clícia Carrijo, Cláudia Braga Pereira Bento, Maria Cristina Baracat-Pereira, Uelinton Manoel Pinto, Leandro Licursi de Oliveira, Maria Cristina Dantas Vanetti
Quorum sensing (QS) is cell-cell communication mechanism mediated by signaling molecules known as autoinducers (AIs) that lead to differential gene expression. Salmonella is unable to synthesize the AI-1 acyl homoserine lactone (AHL), but is able to recognize AHLs produced by other microorganisms through SdiA protein. Our study aimed to evaluate the influence of AI-1 on the abundance of proteins and the levels of organic acids of Salmonella Enteritidis. The presence of N-dodecyl-homoserine lactone (C12-HSL) did not interfere on the growth or the total amount of extracted proteins of Salmonella...
January 2017: Microbial Pathogenesis
Sneha S Garge, Anuradha S Nerurkar
Quorum sensing (QS) is a mechanism in which Gram negative bacterial pathogens sense their population density through acyl homoserine lactones (AHLs) and regulate the expression of virulence factors. Enzymatic degradation of AHLs by lactonases, known as quorum quenching (QQ), is thus a potential strategy for attenuating QS regulated bacterial infections. We characterised the QQ activity of soil isolate Lysinibacillus sp. Gs50 and explored its potential for controlling bacterial soft rot of crop plants. Lysinibacillus sp...
2016: PloS One
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