keyword
MENU ▼
Read by QxMD icon Read
search

AHLS

keyword
https://www.readbyqxmd.com/read/29130184/generation-of-high-sensitivity-monoclonal-antibodies-specific-for-homoserine-lactones
#1
Soumya Palliyil
A number of bacteria use a class of chemical compounds called acyl-homoserine lactones (AHLs) as quorum sensing (QS) signals to coordinate their behavior at the population level, including pathogens like Pseudomonas aeruginosa. Blocking QS using antibodies is an attractive strategy for infection control as this process takes a central role in P. aeruginosa infections. Here the methods involved in the generation of high sensitivity anti-QS monoclonal antibodies from an immunized sheep phage display antibody library are described...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29130182/a-culture-dependent-method-for-the-identification-of-quorum-quenching-enzymes-of-microbial-origin
#2
Kaihao Tang, Xiao-Hua Zhang
Although it has been more than a decade since the first discovery of AHL lactonase AiiA in Bacillus sp. 240B1, we are only beginning to understand the diversity of quorum quenching (QQ) enzymes. Most of the previously identified QQ enzymes are derived from nonmarine microorganisms. A novel marine-derived secretory AHL lactonase, MomL, was found in Muricauda olearia in our previous work and represents a novel type of AHL lactonase widespread in the ocean. Herein, we describe a culture-dependent method for the identification of microbial QQ enzymes, especially the high-throughput method for screening QQ bacteria from cultivable bacterial strains...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29130175/imaging-n-acyl-homoserine-lactone-quorum-sensing-in-vivo
#3
Louise Dahl Hultqvist, Maria Alhede, Tim Holm Jakobsen, Michael Givskov, Thomas Bjarnsholt
In order to study N-acyl homoserine lactone (AHL)-based quorum sensing in vivo, we present a protocol using an Escherichia coli strain equipped with a luxR-based monitor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice challenged intratracheally with alginate beads containing both a Pseudomonas aeruginosa strain together with the E. coli monitor strain can be investigated at different time points postinfection. Epifluorescent or confocal scanning laser microscopy (CSLM) is used to detect the GFP-expressing E...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29130174/identification-of-ahl-and-bdsf-controlled-proteins-in-burkholderia-cenocepacia-by-proteomics
#4
Yilei Liu, Gabriella Pessi, Katharina Riedel, Leo Eberl
We used comparative proteome analysis to determine the target genes of the two quorum sensing (QS) circuits in the opportunistic pathogen Burkholderia cenocepacia: the N-acyl homoserine lactone (AHL)-based CepIR system and the BDSF (B urkholderia diffusible signal factor, cis-2-dodecenoic acid)-based RpfFR system. In this book chapter, we focus on the description of the practical procedure we currently use in the laboratory to perform a sensitive GeLC-MS/MS shotgun proteomics experiment; we also briefly describe the downstream bioinformatic data analysis...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29130172/enzymatic-assays-to-investigate-acyl-homoserine-lactone-autoinducer-synthases
#5
Daniel Shin, Rajesh Nagarajan
Bacteria use chemical molecules called autoinducers as votes to poll their numerical strength in a colony. This polling mechanism, commonly referred to as quorum sensing, enables bacteria to build a social network and provide a collective response for fighting off common threats. In Gram-negative bacteria, AHL synthases synthesize acyl-homoserine lactone (AHL) autoinducers to turn on the expression of several virulent genes including biofilm formation, protease secretion, and toxin production. Therefore, inhibiting AHL signal synthase would limit quorum sensing and virulence...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29130163/liquid-chromatography-mass-spectrometry-lc-ms-for-the-detection-and-quantification-of-n-acyl-l-homoserine-lactones-ahls-and-4-hydroxy-2-alkylquinolines-haqs
#6
François Lépine, Sylvain Milot, Marie-Christine Groleau, Eric Déziel
High-performance liquid chromatography (HPLC) coupled in-line with mass spectrometry (MS) permits rapid and specific identification and quantification of N-acyl-L-homoserine lactones (AHLs) and 4-hydroxy-2-alkylquinolines (HAQs). We are presenting here methods for the analysis of these molecules directly from biological samples using LC/MS.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29130162/-hot-stuff-the-many-uses-of-a-radiolabel-assay-in-detecting-acyl-homoserine-lactone-quorum-sensing-signals
#7
Amy L Schaefer, Caroline S Harwood, E Peter Greenberg
Many Proteobacteria synthesize acyl-homoserine lactone (AHL) molecules for use as signals in cell density-dependent gene regulation known as quorum sensing (QS) and response. AHL detection protocols are essential to QS researchers and several techniques are available, including a (14)C-AHL radiolabel assay. This assay is based on the uptake of radiolabeled methionine by living cells and conversion of the radiolabel into S-adenosylmethionine (SAM). The radiolabeled SAM is then incorporated into AHL signal by an AHL synthase enzyme...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29130160/use-of-whole-cell-bioassays-for-screening-quorum-signaling-quorum-interference-and-biofilm-dispersion
#8
Starla G Thornhill, Robert J C McLean
In most bacteria, a global level of regulation, termed quorum sensing (QS), exists involving intercellular communication via the production and response to cell density-dependent signal molecules. QS has been associated with a number of important features in bacteria including virulence regulation and biofilm formation. Consequently, there is considerable interest in understanding, detecting, and inhibiting QS. N-acylated homoserine lactones (AHLs) are used as extracellular QS signals by a variety of Gram-negative bacteria...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29125368/societal-burden-of-cluster-headache-in-the-united-states-a-descriptive-economic-analysis
#9
Janet H Ford, Damion Nero, Gilwan Kim, Bong Chul Chu, Robert Fowler, Jonna Ahl, James M Martinez
AIM: To estimate direct and indirect costs in patients with a diagnosis of cluster headache in the US. METHODS: Adult patients (18 - 64 years of age) enrolled in the Marketscan Commercial and Medicare Databases with ≥ 2 non-diagnostic outpatient (≥ 30 days apart between the two outpatient claims) or ≥ 1 inpatient diagnoses of cluster headache (ICD-9-CM code 339.00, 339.01, or 339.02) between January 1, 2009 and June 30, 2014, were included in the analyses...
November 10, 2017: Journal of Medical Economics
https://www.readbyqxmd.com/read/29098138/antiquorum-sensing-activity-of-silver-nanoparticles-in-p-aeruginosa-an-in-silico-study
#10
Syed Ghazanfar Ali, Mohammad Azam Ansari, Qazi Mohd Sajid Jamal, Haris M Khan, Mohammad Jalal, Hilal Ahmad, Abbas Ali Mahdi
Pseudomonas aeruginosa an opportunistic pathogen regulates its virulence through Quorum sensing (QS) mechanism comprising of Las and Rhl system. Targeting of QS mechanism could be an ideal strategy to combat infection caused by P. aeruginosa. Silver nanoparticles (AgNPs) have been broadly applied as antimicrobial agents against a number of pathogenic bacterial and fungal strains, but have not been reported as an anti-QS agent. Therefore, the aim of present work was to show the computational analysis for the interaction of AgNPs with the QS system using an In silico approach...
2017: In Silico Pharmacology
https://www.readbyqxmd.com/read/29073163/the-usefulness-of-appetite-and-energy-intake-based-algorithms-to-assess-treatment-effect-of-a-bacterial-infection-an-observational-prospective-study
#11
Viktor Peny, Fredrik Månsson, Fredrik Resman, Jonas Ahl, Johan Tham
BACKGROUND: The diagnosis of infectious diseases and the duration of antibiotic therapies are generally based on empirical rules. Studies implicate that the use biological markers can be used as a reliable method to shorten antibiotic therapies. The return of appetite is a clinical aspect of recovery from an infection that may be used to guide antibiotic therapies. OBJECTIVE: To compare changes in appetite and daily energy intake with changes in CRP-levels in patients recovering from an infection...
2017: PloS One
https://www.readbyqxmd.com/read/29071963/-effect-of-electroacupuncture-on-expression-of-catechol-o-methyltransferase-in-the-inferior-colliculus-and-auditory-cortex-in-age-related-hearing-loss-guinea-pigs
#12
Shu-Yun Liu, Li-Qiang Deng, Ye Yang, Ze-Deng Yin
OBJECTIVE: To observe the expression of catechol-O-methyltransferase (COMT) in inferior colliculus and auditory cortex of guinea pigs with age-related hearing loss(AHL) induced by D-galactose, so as to explore the possible mechanism of electroacupuncture(EA) underlying preventing AHL. METHODS: Thirty 3-month-old guinea pigs were randomly divided into control group, model group and EA group(n=10 in each group), and ten 18-month-old guinea pigs were allocated as elderly group...
April 25, 2017: Zhen Ci Yan Jiu, Acupuncture Research
https://www.readbyqxmd.com/read/29066541/pseudomonas-syringae-pv-syringae-b728a-regulates-multiple-stages-of-plant-colonization-via-the-bacteriophytochrome-bphp1
#13
Regina McGrane, Gwyn A Beattie
Light may be an important environmental signal for plant-associated bacteria, particularly those that live on leaves. An integrated network of red/far-red- and blue-light-responsive photosensory proteins is known to inhibit swarming motility in the foliar plant pathogen Pseudomonas syringae pv. syringae B728a. Here we elucidated factors in the red/far-red-light-sensing bacteriophytochrome BphP1 signal transduction pathway and report evidence for a role of BphP1 in multiple stages of the Psyringae B728a life cycle...
October 24, 2017: MBio
https://www.readbyqxmd.com/read/29049387/quantifying-the-strength-of-quorum-sensing-crosstalk-within-microbial-communities
#14
Kalinga Pavan T Silva, Prithiviraj Chellamuthu, James Q Boedicker
In multispecies microbial communities, the exchange of signals such as acyl-homoserine lactones (AHL) enables communication within and between species of Gram-negative bacteria. This process, commonly known as quorum sensing, aids in the regulation of genes crucial for the survival of species within heterogeneous populations of microbes. Although signal exchange was studied extensively in well-mixed environments, less is known about the consequences of crosstalk in spatially distributed mixtures of species...
October 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/29048177/the-role-of-a-palliative-care-intervention-in-moderating-the-relationship-between-depression-and-survival-among-individuals-with-advanced-cancer
#15
Anna T Prescott, Jay G Hull, J Nicholas Dionne-Odom, Tor D Tosteson, Kathleen Doyle Lyons, Zhigang Li, Zhongze Li, Konstantin H Dragnev, Mark T Hegel, Karen E Steinhauser, Tim A Ahles, Marie A Bakitas
OBJECTIVE: Randomized controlled trials (RCTs) of early palliative care interventions in advanced cancer have positively impacted patient survival, yet the mechanisms remain unknown. This secondary analysis of 2 RCTs assessed whether an early palliative care intervention moderates the relationship between depressive symptoms and survival. METHOD: The relationships among mood, survival, and early palliative care intervention were studied among 529 advanced cancer patients who participated in 2 RCTs...
October 19, 2017: Health Psychology: Official Journal of the Division of Health Psychology, American Psychological Association
https://www.readbyqxmd.com/read/29044730/cognition-and-cancer-conceptual-and-methodological-issues-and-future-directions
#16
Tim A Ahles, Arti Hurria
No abstract text is available yet for this article.
October 17, 2017: Psycho-oncology
https://www.readbyqxmd.com/read/29035934/dextrometorphan-paroxetine-but-not-dronabinol-effective-for-treatment-resistant-aggression-and-agitation-in-an-elderly-patient-with-lewy-body-dementia
#17
Robert Wilhelm, Björn Ahl, Ion-George Anghelescu
No abstract text is available yet for this article.
October 13, 2017: Journal of Clinical Psychopharmacology
https://www.readbyqxmd.com/read/28993333/antibiotics-promote-escherichia-coli-pseudomonas-aeruginosa-conjugation-through-inhibiting-quorum-sensing
#18
Yang Lu, Jianming Zeng, Linjing Wang, Kai Lan, E Shunmei, Lina Wang, Qian Xiao, Qiang Luo, Xianzhang Huang, Bin Huang, Cha Chen
The effect of antibiotics on horizontal gene transfer is controversial and the underlying mechanism remains poorly understood. Here, using E. coli SM10λπ which carries a chromosomally integrated RP4 plasmid as donor strain, we investigated the effect of antibiotics on conjugational transfer of a mobilizable gentamicin (Gm) resistance plasmid. The results showed that exposure to gentamicin that may restrict the survival of recipient cells significantly enhanced SM10λπ-P. aeruginosa (PAO1) conjugation, which was attenuated by deficiency of lasI/rhlI, genes associated with generation of the quorum sensing signals N-acyl homoserine lactones (AHLs) in PAO1, or deletion of the AHLs receptor SdiA in SM10λπ...
October 9, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28984356/a-real-world-analysis-of-migraine-a-cross-sectional-study-of-disease-burden-and-treatment-patterns
#19
Janet H Ford, James Jackson, Gary Milligan, Sarah Cotton, Jonna Ahl, Sheena K Aurora
OBJECTIVE: The purpose of this cross-sectional study was to assess the sociodemographics, disease burden, and treatment patterns of patients with episodic and chronic migraine in the United States. BACKGROUND: Migraine is a disabling neurological disease that places an enormous burden on patients. METHODS: Data were drawn from the Adelphi Migraine United States Disease Specific Programme (index period: January to March 2014). Physicians (N = 150) completed a patient report form on 10 consulting patients with migraine...
October 6, 2017: Headache
https://www.readbyqxmd.com/read/28973119/optic-nerve-infiltration-in-primary-central-nervous-system-lymphoma
#20
Guido Ahle, Valérie Touitou, Nathalie Cassoux, Marie Bouyon, Catherine Humbrecht, Hélène Oesterlé, Alexander Baraniskin, Carole Soussain, Ludovic Nguyen-Them, Claude Gaultier, Khê Hoang-Xuan, Caroline Houillier
Importance: Visual impairment in primary central nervous system lymphoma (PCNSL) is caused mostly by intraocular lymphomatous involvement (vitritis and retinal infiltration), whereas optic nerve infiltration (ONI) is a rare condition. Objective: To describe the clinical presentation of ONI, its imaging characteristics, and outcome. Design, Setting and Participants: A total of 752 patients diagnosed with PCNSL were retrospectively identified from the databases of 3 French hospitals from January 1, 1998, through December 31, 2014...
November 1, 2017: JAMA Neurology
keyword
keyword
119067
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"