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Mesenchymal cell transplantation

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https://www.readbyqxmd.com/read/28810264/mesenchymal-stem-cells-promote-diabetic-corneal-epithelial-wound-healing-through-tsg-6-dependent-stem-cell-activation-and-macrophage-switch
#1
Guohu Di, Xianli Du, Xia Qi, Xiaowen Zhao, Haoyun Duan, Suxia Li, Lixin Xie, Qingjun Zhou
Purpose: To explore the role and mechanism of bone marrow-derived mesenchymal stem cells (BM-MSCs) in corneal epithelial wound healing in type 1 diabetic mice. Methods: Diabetic mice were treated with subconjunctival injections of BM-MSCs or recombinant tumor necrosis factor-α-stimulated gene/protein-6 (TSG-6). The corneal epithelial wound healing rate was examined by fluorescein staining. The mRNA and protein expression levels of TSG-6 were measured by quantitative RT-PCR and Western blot...
August 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28809840/a-novel-clinical-grade-isolation-method-for-human-kidney-perivascular-stromal-cells
#2
Daniëlle G Leuning, Ellen Lievers, Marlies E J Reinders, Cees van Kooten, Marten A Engelse, Ton J Rabelink
Mesenchymal Stromal Cells (MSCs) are tissue homeostatic and immune modulatory cells that have shown beneficial effects in kidney diseases and transplantation. Perivascular Stromal Cells (PSCs) share characteristics with bone marrow MSCs (bmMSCs). However, they also possess, most likely due to local imprinting, tissue-specific properties and play a role in local tissue homeostasis. This tissue specificity may result in tissue specific repair, also within the human kidney. We previously showed that human kidney PSCs (kPSCs) have enhanced kidney epithelial wound healing whereas bmMSCs did not have this potential...
August 7, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28809824/creation-and-transplantation-of-an-adipose-derived-stem-cell-asc-sheet-in-a-diabetic-wound-healing-model
#3
Yuka Kato, Takanori Iwata, Kaoru Washio, Toshiyuki Yoshida, Hozue Kuroda, Shunichi Morikawa, Mariko Hamada, Kazuki Ikura, Nobuyuki Kaibuchi, Masayuki Yamato, Teruo Okano, Yasuko Uchigata
Artificial skin has achieved considerable therapeutic results in clinical practice. However, artificial skin treatments for wounds in diabetic patients with impeded blood flow or with large wounds might be prolonged. Cell-based therapies have appeared as a new technique for the treatment of diabetic ulcers, and cell-sheet engineering has improved the efficacy of cell transplantation. A number of reports have suggested that adipose-derived stem cells (ASCs), a type of mesenchymal stromal cell (MSC), exhibit therapeutic potential due to their relative abundance in adipose tissue and their accessibility for collection when compared to MSCs from other tissues...
August 4, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28807003/overexpression-of-mir-21-in-stem-cells-improves-ovarian-structure-and-function-in-rats-with-chemotherapy-induced-ovarian-damage-by-targeting-pdcd4-and-pten-to-inhibit-granulosa-cell-apoptosis
#4
Xiafei Fu, Yuanli He, Xuefeng Wang, Dongxian Peng, Xiaoying Chen, Xinran Li, Qing Wang
BACKGROUND: Chemotherapy-induced premature ovarian failure (POF) is a severe complication affecting tumor patients at a childbearing age. Mesenchymal stem cells (MSCs) can partially restore the ovarian structure and function damaged by chemotherapy. miR-21 is a microRNA that can regulate cell apoptosis. This study discusses the repair effect and mechanism of MSCs overexpressing miR-21 on chemotherapy-induced POF. METHODS: Rat MSCs and granulosa cells (GCs) were isolated in vitro...
August 14, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28805297/mesenchymal-stem-cells-deliver-exogenous-mir-21-via-exosomes-to-inhibit-nucleus-pulposus-cell-apoptosis-and-reduce-intervertebral-disc-degeneration
#5
Xiaofei Cheng, Guoying Zhang, Liang Zhang, Ying Hu, Kai Zhang, Xiaojiang Sun, Changqing Zhao, Hua Li, Yan Michael Li, Jie Zhao
Although mesenchymal stem cells (MSCs) transplantation into the IVD (intervertebral disc) may be beneficial in inhibiting apoptosis of nucleus pulposus cells (NPCs) and alleviating IVD degeneration, the underlying mechanism of this therapeutic process has not been fully explained. The purpose of this study was to explore the protective effect of MSC-derived exosomes (MSC-exosomes) on NPC apoptosis and IVD degeneration and investigate the regulatory effect of miRNAs in MSC-exosomes and associated mechanisms for NPC apoptosis...
August 14, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28804540/restoration-of-bone-defects-using-modified-heterogeneous-deproteinized-bone-seeded-with-bone-marrow-mesenchymal-stem-cells
#6
Jun Li, Zeyu Huang, Liyan Chen, Xin Tang, Yue Fang, Lei Liu
The aim of the present study was to investigate the effect of modified heterogeneous deproteinized bone combined with bone marrow mesenchymal stem cells (BMSCs) in the restoration of a validated bone defect model. BMSCs were identified by flow cytometry and multilineage differentiation assay. The structural features of the modified heterogeneous deproteinized bone scaffold and biocompatibility between BMSCs and the scaffold were confirmed by scanning electron microscope (SEM) detection. The cytotoxicity of the modified heterogeneous deproteinized bone scaffolds were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenytetrazolium bromide (MTT) assay...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28801687/human-mesenchymal-stromal-cell-sheet-enhances-allograft-repair-in-a-mouse-model
#7
Xifu Shang, Bing Shu, Yongjun Wang, Zhengliang Luo, Guangxi Wang, Shane Barton, Massimo Max Morandi, Christopher Kevil, Yufeng Dong
To determine whether cell sheets generated with long-term passaged (P10) aging human mesenchymal stromal cells (MSCs) could be used for bone tissue regeneration as tissue engineered periosteum in a femoral allograft mouse model similar to fresh passaged (P3) young MSCs. At 3 weeks after transplantation of MSC sheets, results showed more bony callus formed between allograft and host bone ends in both young P3 MSC and aged P10 MSC sheet-wrapped groups when compared to allograft alone. At 6 weeks, while both MSC sheet-wrapped allografts showed more bony callus formation when compared to allograft alone groups, the bony callus size in aged P10 MSC sheet groups was significantly less than young P3 MSC sheet groups...
August 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28796340/application-of-differentiated-human-tonsil-derived-stem-cells-to-trembler-j-mice
#8
Saeyoung Park, Yoonyoung Choi, Geon Kwak, Young Bin Hong, Namhee Jung, Jieun Kim, Byung-Ok Choi, Sung-Chul Jung
INTRODUCTION: Mesenchymal stem cells (MSCs) can differentiate into various cell types. METHODS: We investigated the potential of human tonsil-derived MSCs (T-MSCs) for neuromuscular regeneration in trembler-J (Tr-J) mice: a model for Charcot-Marie-Tooth disease type 1A (CMT1A. RESULTS: T-MSCs differentiated toward skeletal myocytes with increased expression of skeletal muscle-related markers (including troponin I type 1, and myogenin), and the formation of myotubes in vitro...
August 10, 2017: Muscle & Nerve
https://www.readbyqxmd.com/read/28796260/clinical-grade-human-umbilical-cord-derived-mesenchymal-stem-cells-reverse-cognitive-aging-via-improving-synaptic-plasticity-and-endogenous-neurogenesis
#9
Ning Cao, Tuling Liao, Jiajing Liu, Zeng Fan, Quan Zeng, Junnian Zhou, Haiyun Pei, Jiafei Xi, Lijuan He, Lin Chen, Xue Nan, Yali Jia, Wen Yue, Xuetao Pei
Cognitive aging is a leading public health concern with the increasing aging population, but there is still lack of specific interventions directed against it. Recent studies have shown that cognitive function is intimately affected by systemic milieu in aging brain, and improvement of systemic environment in aging brain may be a promising approach for rejuvenating cognitive aging. Here, we sought to study the intervention effects of clinical-grade human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) on cognitive aging in a murine model of aging...
August 10, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28794794/sfrp2-enhances-the-osteogenic-differentiation-of-apical-papilla-stem-cells-by-antagonizing-the-canonical-wnt-pathway
#10
Luyuan Jin, Yu Cao, Guoxia Yu, Jinsong Wang, Xiao Lin, Lihua Ge, Juan Du, Liping Wang, Shu Diao, Xiaomeng Lian, Songlin Wang, Rui Dong, Zhaochen Shan
BACKGROUND: Exploring the molecular mechanisms underlying directed differentiation is helpful in the development of clinical applications of mesenchymal stem cells (MSCs). Our previous study on dental tissue-derived MSCs demonstrated that secreted frizzled-related protein 2 (SFRP2), a Wnt inhibitor, could enhance osteogenic differentiation in stem cells from the apical papilla (SCAPs). However, how SFRP2 promotes osteogenic differentiation of dental tissue-derived MSCs remains unclear...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28793833/synergistic-therapeutic-effect-of-three-dimensional-stem-cell-clusters-and-angiopoietin-1-on-promoting-vascular-regeneration-in-ischemic-region
#11
Jungmi Kang, Jeong-Kee Yoon, Seung Ja Oh, Byung-Soo Kim, Sang-Heon Kim
Peripheral artery disease (PAD) is an ischemic disease characterized by reduced blood flow to the legs, feet, and hands. Human mesenchymal stem cells (MSCs) are an attractive cell source to treat PAD in regenerative medicine. However, in clinical applications, the use of adult stem cells has several limitations, such as low cell viability and low therapeutic efficiency. In the present study, we described an innovative method of culturing three-dimensional stem cell clusters (AngioclusterTM, AC), demonstrated the potential for ACs to differentiate into vascular cells, and assessed the synergistic effects of ACs and angiopoietin-1 (Ang-1) on angiogenesis in ischemic animal models...
August 10, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28777501/liposome-indocyanine-green-nanoprobes-for-optical-labeling-and-tracking-of-human-mesenchymal-stem-cells-post-transplantation-in-vivo
#12
Mariarosa Mazza, Neus Lozano, Debora Braga Vieira, Maurizio Buggio, Cay Kielty, Kostas Kostarelos
Direct labeling of human mesenchymal stem cells (hMSC) prior to transplantation provides a means to track cells after administration and it is a powerful tool for the assessment of new cell-based therapies. Biocompatible nanoprobes consisting of liposome-indocyanine green hybrid vesicles (liposome-ICG) are used to safely label hMSC. Labeled hMSC recapitulating a 3D cellular environment is transplanted as spheroids subcutaneously and intracranially in athymic nude mice. Cells emit a strong NIR signal used for tracking post-transplantation with the IVIS imaging system up to 2 weeks (subcutaneous) and 1 week (intracranial)...
August 4, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28776332/sirolimus-and-metformin-synergistically-inhibits-colon-cancer-in-vitro-and-in-vivo
#13
Nadiar Mussin, Seung Cheol Oh, Kwang Woong Lee, Min Young Park, Sooin Seo, Nam Joon Yi, Hyeyoung Kim, Kyung Chul Yoon, Sung Woo Ahn, Hyo Sin Kim, Suk Kyun Hong, Dong Kyu Oh, Kyung Suk Suh
We estimated the effect of various immunosuppressants (ISs) and metformin (M) to provide theoretical background of optimal therapeutic strategy for de novo colon cancer after liver transplantation (LT). Three colon cancer cell lines (HT29, SW620, and HCT116) were used in in vitro studies. HT29 was also used in BALB/c-nude mice animal models. Following groups were used in both in vitro and in vivo studies: sirolimus (S), tacrolimus (T), cyclosporin A (CsA), M, metformin/sirolimus (Met/S), metformin/tacrolimus (Met/T), and metformin/cyclosporin A (Met/CsA)...
September 2017: Journal of Korean Medical Science
https://www.readbyqxmd.com/read/28774590/human-adipose-mesenchymal-cells-inhibit-melanocyte-differentiation-and-the-pigmentation-of-human-skin-via-increased-expression-of-tgf-%C3%AE-1
#14
Agnes S Klar, Thomas Biedermann, Katarzyna Michalak, Teresa Michalczyk, Claudia Meuli-Simmen, Arnaud Scherberich, Martin Meuli, Ernst Reichmann
There is accumulating evidence that interactions between epidermal melanocytes and stromal cells play an important role in the regulation of skin pigmentation. In this study we established a pigmented dermo-epidermal skin model (melDESS) of human origin to investigate the effects of distinct stromal cells on melanogenesis. melDESS is a complex, clinically relevant skin equivalent composed of an epidermis containing both melanocytes and keratinocytes. Its dermal compartment consisted either of adipose tissue-derived stromal cells (ASC), dermal fibroblasts, or a mixture of both cell types...
July 31, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28774569/the-condition-medium-of-mesenchymal-stem-cells-promotes-proliferation-adhesion-and-neuronal-differentiation-of-retinal-progenitor-cells
#15
Mingqi Zhang, Fenglei Zhang, Jin Sun, Yan Sun, Ling Xu, Donglei Zhang, Zhuoshi Wang, Wei He
Retinal progenitor cell is a promising candidate in the treatment of retinal pigmentosa diseases. The limiting factors of stem cell transplantation are the proliferation and differentiation capacities of hRPCs, which may be governed by culture conditions. Previous studies have proved that the secretome of human Umbilical Cord Mesenchymal stem cells (hUCMSCs) and human Adipose derived stem cells (hADSCs), including more active cytokines and neurotrophic factors, have the paracrine potential of enhancing proliferation and differentiation in several cell types...
July 31, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28771731/repairing-the-vibratory-vocal-fold
#16
Jennifer L Long
OBJECTIVE: A vibratory vocal fold replacement would introduce a new treatment paradigm for structural vocal fold diseases such as scarring and lamina propria loss. This work implants a tissue-engineered replacement for vocal fold lamina propria and epithelium in rabbits and compares histology and function to injured controls and orthotopic transplants. Hypotheses were that the cell-based implant would engraft and control the wound response, reducing fibrosis and restoring vibration. STUDY DESIGN: Translational research...
August 3, 2017: Laryngoscope
https://www.readbyqxmd.com/read/28769981/human-suprapatellar-fat-pad-derived-mesenchymal-stem-cells-induce-chondrogenesis-and-cartilage-repair-in-a-model-of-severe-osteoarthritis
#17
Ignacio Muñoz-Criado, Jose Meseguer-Ripolles, Maravillas Mellado-López, Ana Alastrue-Agudo, Richard J Griffeth, Jerónimo Forteza-Vila, Ramón Cugat, Montserrat García, Victoria Moreno-Manzano
Cartilage degeneration is associated with degenerative bone and joint processes in severe osteoarthritis (OA). Spontaneous cartilage regeneration is extremely limited. Often the treatment consists of a partial or complete joint implant. Adipose-derived stem cell (ASC) transplantation has been shown to restore degenerated cartilage; however, regenerative differences of ASC would depend on the source of adipose tissue. The infra- and suprapatellar fat pads surrounding the knee offer a potential autologous source of ASC for patients after complete joint substitution...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28769980/galectin-3-knockdown-impairs-survival-migration-and-immunomodulatory-actions-of-mesenchymal-stromal-cells-in-a-mouse-model-of-chagas-disease-cardiomyopathy
#18
Bruno Solano de Freitas Souza, Kátia Nunes da Silva, Daniela Nascimento Silva, Vinícius Pinto Costa Rocha, Bruno Diaz Paredes, Carine Machado Azevedo, Carolina Kymie Nonaka, Gisele Batista Carvalho, Juliana Fraga Vasconcelos, Ricardo Ribeiro Dos Santos, Milena Botelho Pereira Soares
Therapies based on transplantation of mesenchymal stromal cells (MSC) hold promise for the management of inflammatory disorders. In chronic Chagas disease cardiomyopathy (CCC), caused by chronic infection with Trypanosoma cruzi, the exacerbated immune response plays a critical pathophysiological role and can be modulated by MSC. Here, we investigated the role of galectin-3 (Gal-3), a beta-galactoside-binding lectin with several actions on immune responses and repair process, on the immunomodulatory potential of MSC...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28769083/human-adipose-derived-mesenchymal-stem-cells-seeded-into-a-collagen-hydroxyapatite-scaffold-promote-bone-augmentation-after-implantation-in-the-mouse
#19
Giovanna Calabrese, Raffaella Giuffrida, Stefano Forte, Claudia Fabbi, Elisa Figallo, Lucia Salvatorelli, Lorenzo Memeo, Rosalba Parenti, Massimo Gulisano, Rosario Gulino
Traumatic injury or surgical excision of diseased bone tissue usually require the reconstruction of large bone defects unable to heal spontaneously, especially in older individuals. This is a big challenge requiring the development of biomaterials mimicking the bone structure and capable of inducing the right commitment of cells seeded within the scaffold. In particular, given their properties and large availability, the human adipose-derived stem cells are considered as the better candidate for autologous cell transplantation...
August 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28768234/human-mesenchymal-stem-cells-ameliorate-experimental-pulmonary-hypertension-induced-by-maternal-inflammation-and-neonatal-hyperoxia-in-rats
#20
Chung-Ming Chen, Willie Lin, Liang-Ti Huang, Hsiu-Chu Chou
Pulmonary hypertension is a critical problem in infants with bronchopulmonary dysplasia. This study determined the therapeutic effects of human mesenchymal stem cells (MSCs) on pulmonary hypertension in an animal model. Pregnant Sprague-Dawley rats were intraperitoneally injected with lipopolysaccharide (LPS, 0.5 mg/kg/day) on gestational days 20 and 21. The pups were randomly assigned to two treatment conditions: room air (RA) or an O2-enriched atmosphere. On postnatal day 5, they were intratracheally transplanted with human MSCs (3 × 105 and 1 × 106 cells) in 0...
July 19, 2017: Oncotarget
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