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https://www.readbyqxmd.com/read/27868067/purification-and-characterization-of-a-thermostable-%C3%AE-mannanase-from-bacillus-subtilis-be-91-potential-application-in-inflammatory-diseases
#1
Lifeng Cheng, Shengwen Duan, Xiangyuan Feng, Ke Zheng, Qi Yang, Zhengchu Liu
β-mannanase has shown compelling biological functions because of its regulatory roles in metabolism, inflammation, and oxidation. This study separated and purified the β-mannanase from Bacillus subtilis BE-91, which is a powerful hemicellulose-degrading bacterium using a "two-step" method comprising ultrafiltration and gel chromatography. The purified β-mannanase (about 28.2 kDa) showed high specific activity (79, 859.2 IU/mg). The optimum temperature and pH were 65°C and 6.0, respectively. Moreover, the enzyme was highly stable at temperatures up to 70°C and pH 4...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27842852/characteristics-of-glucomannan-isolated-from-fresh-tuber-of-porang-amorphophallus-muelleri-blume
#2
Anny Yanuriati, Djagal Wiseso Marseno, Rochmadi, Eni Harmayani
Porang is a potential source of glucomannan. This research objective was to find a direct glucomannan isolation method from fresh porang corm to produce high purity glucomannan. Two isolation methods were performed. In first method, sample was water dissolved using Al2(SO4)3 as flocculant for 15 (AA15) or 30 (AA30) minutes with purification. In second method, sample was repeatedly milled using ethanol as solvent and filtered for 5 (EtOH5) or 7 (EtOH7) times without purification. The characteristics of obtained glucomannan were compared to those of commercial porang flour (CPF) and purified konjac glucomannan (PKG)...
January 20, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27835835/effect-of-natural-antioxidants-in-spanish-salchich%C3%A3-n-elaborated-with-encapsulated-n-3-long-chain-fatty-acids-in-konjac-glucomannan-matrix
#3
P E S Munekata, R Domínguez, D Franco, R Bermúdez, M A Trindade, Jose M Lorenzo
The effect of natural antioxidants on physicochemical properties, lipid and protein oxidation, volatile compounds and free fatty acids (FFA) were determined in Spanish salchichón enriched with n-3 fatty acids encapsulated and stabilized in konjac matrix. Phenolic compounds of beer residue extract (BRE), chestnut leaves extract (CLE) and peanut skin extract (PSE) were also identified and quantified. Five batches of salchichón were prepared: control (CON, without antioxidants), butylated hydroxytoluene (BHT), BRE, CLE and PSE...
November 5, 2016: Meat Science
https://www.readbyqxmd.com/read/27819518/x-ray-induced-mutation-of-bacillus-sp-mr10-for-manno-oligosaccharides-production-from-copra-meal
#4
Siriporn Chaikaew, Apinun Kanpiengjai, Jenjira Intatep, Kridsada Unban, Goro Takata, Chartchai Khanongnuch
The present study demonstrates the effectiveness of x-ray radiation in strain improvement for defective lipase production by Bacillus sp. MR10 for further application in the fermentative production of manno-oligosaccharides (MOS) from agricultural by-product, defatted copra meal (DCM). The mutants obtained were screened based on their defective lipase activity together with their β-mannanase production performance. Among ten selected mutants, the strain M7 was the highest promising mutant regarding the smallest lipase activity (0...
November 7, 2016: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/27773606/engineering-a-family-27-carbohydrate-binding-module-into-an-aspergillus-usamii-%C3%AE-mannanase-to-perfect-its-enzymatic-properties
#5
Jianfang Li, Chunjuan Wang, Die Hu, Fengjiao Yuan, Xueqing Li, Shihan Tang, Minchen Wu
A family 27 carbohydrate-binding module of a Thermotoga maritima β-mannanase (TmCBM27) was chosen from the carbohydrate-active enzyme database by computer-aided design, possessing the lowest binding free energy with mannopentaose. To improve the enzymatic properties of a glycoside hydrolase family 5 β-mannanase from Aspergillus usamii (AuMan5A), two fusion β-mannanases, AuMan5A-F-M and AuMan5A-R-M, were designed by fusing a TmCBM27 into its C-terminus linked with a flexible peptide F (GGGGS)3 and rigid peptide R (EAAAK)3...
October 20, 2016: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/27771411/da-kgm-based-go-reinforced-fmbo-loaded-aerogels-for-efficient-arsenic-removal-in-aqueous-solution
#6
Shuxin Ye, Weiping Jin, Qing Huang, Ying Hu, Yan Li, Jing Li, Bin Li
Composites based on deacetylated konjac glucomannan (Da-KGM) and graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water. Da-KGM, which was used as supporting composite matrix here, were firstly treated with GO and loaded FMBO. The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM)...
October 19, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27709705/undisclosed-presence-of-methylisothiazolinone-in-100-natural-konjac%C3%A2-sponge
#7
Jakob T Madsen, Klaus E Andersen, Dorte T Nielsen, Lone Hvid, Rime B El-Houri, Lars P Christensen
No abstract text is available yet for this article.
November 2016: Contact Dermatitis
https://www.readbyqxmd.com/read/27706095/soluble-fiber-with-high-water-binding-capacity-swelling-capacity-and-fermentability-reduces-food-intake-by-promoting-satiety-rather-than-satiation-in-rats
#8
Chengquan Tan, Hongkui Wei, Xichen Zhao, Chuanhui Xu, Yuanfei Zhou, Jian Peng
To understand whether soluble fiber (SF) with high water-binding capacity (WBC), swelling capacity (SC) and fermentability reduces food intake and whether it does so by promoting satiety or satiation or both, we investigated the effects of different SFs with these properties on the food intake in rats. Thirty-two male Sprague-Dawley rats were randomized to four equal groups and fed the control diet or diet containing 2% konjac flour (KF), pregelatinized waxy maize starch (PWMS) plus guar gum (PG), and PWMS starch plus xanthan gum (PX) for three weeks, with the measured values of SF, WBC, and SC in the four diets following the order of PG > KF > PX > control...
October 2, 2016: Nutrients
https://www.readbyqxmd.com/read/27702490/genome-mining-and-motif-truncation-of-glycoside-hydrolase-family-5-endo-%C3%AE-1-4-mannanase-encoded-by-aspergillus-oryzae-rib40-for-potential-konjac-flour-hydrolysis-or-feed-additive
#9
Cun-Duo Tang, Hong-Ling Shi, Qing-Hai Tang, Jun-Shi Zhou, Lun-Guang Yao, Zhu-Jin Jiao, Yun-Chao Kan
Two novel glycosyl hydrolase family 5 (GH5) β-mannanases (AoMan5A and AoMan5B) were identified from Aspergillus oryzae RIB40 by genome mining. The AoMan5A contains a predicted family 1 carbohydrate binding module (CBM-1), located at its N-terminal. The AoMan5A, AoMan5B and truncated mutant AoMan5AΔCL (truncating the N-terminal CBM and linker of AoMan5A) were expressed retaining the N-terminus of the native protein in Pichia pastoris GS115 by pPIC9K(M). The specific enzyme activity of the purified reAoMan5A, reAoMan5B and reAoMan5AΔCL towards locust bean gum at pH 3...
November 2016: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/27694039/the-effects-of-konjac-oligosaccharide-on-tnbs-induced-colitis-in-rats
#10
Ruixue Liu, Yongchao Li, Bo Zhang
The purpose of the study was to assess the effects and the protective mechanism of konjac oligosaccharide (KOS) on the ulcerative colitis (UC) model induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) in rats. KOS (1.0 and 4.0g/kg/day) was administered for 14days after the induction of colitis with TNBS. The status of the rats was assessed by morphological and biochemical methods. The effect of KOS on the colonic microflora was also assessed by studying the bacteria profile and short chain fatty acids (SCFAs) production in feces by standard culture techniques and gas chromatography, respectively...
September 29, 2016: International Immunopharmacology
https://www.readbyqxmd.com/read/27615677/ultralight-biomass-derived-carbonaceous-nanofibrous-aerogels-with-superelasticity-and-high-pressure-sensitivity
#11
Yang Si, Xueqin Wang, Chengcheng Yan, Liu Yang, Jianyong Yu, Bin Ding
Superelastic and pressure-sensitive carbonaceous nanofibrous aerogels with a honeycomb-like structure are fabricated through the combination of sustainable konjac glucomannan biomass and flexible SiO2 nanofibers. The aerogels can detect dynamic pressure with a wide pressure range and high sensitivity, which enables real pressure signals such as human blood pulses to be monitored in real time and in situ.
September 12, 2016: Advanced Materials
https://www.readbyqxmd.com/read/27521845/the-control-of-ice-crystal-growth-and-effect-on-porous-structure-of-konjac-glucomannan-based-aerogels
#12
Xuewen Ni, Fan Ke, Man Xiao, Kao Wu, Ying Kuang, Harold Corke, Fatang Jiang
Konjac glucomannan (KGM)-based aerogels were prepared using a combination of sol-gel and freeze-drying methods. Preparation conditions were chosen to control ice crystal growth and aerogel structure formation. The ice crystals formed during pre-freezing were observed by low temperature polarizing microscopy, and images of aerogel pores were obtained by scanning electron microscopy. The size of ice crystals were calculated and size distribution maps were drawn, and similarly for aerogel pores. Results showed that ice crystal growth and aerogel pore sizes may be controlled by varying pre-freezing temperatures, KGM concentration and glyceryl monostearate concentration...
November 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27492560/kgm-based-magnetic-carbon-aerogels-matrix-for-the-uptake-of-methylene-blue-and-methyl-orange
#13
Shuxin Ye, Weiping Jin, Qing Huang, Ying Hu, Yan Li, Bin Li
In this study, the preparation of magnetic Fe and Mn oxides (Mag-FMBO) loaded carbon aerogels (CA) based on konjac glucomannan (KGM) and their performance for dyes adsorption were investigated. The prepared magnetic carbon aerogels (Mag-CA) materials were characterized by various methods, including BET surface area analysis, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and vibrating sample magnetometer (VSM). What's more, the Mag-CA materials were used as adsorbents to remove dyes [anionic methyl orange (MO) and cationic methylene blue (MB)] from aqueous solutions...
November 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27481345/konjac-glucomannan-a-promising-polysaccharide-of-amorphophallus-konjac-k-koch-in-health-care
#14
Sudhanshu S Behera, Ramesh C Ray
In recent year, konjac glucomannan (KGM) has attracted more attention due to its non-harmful and non-toxic properties, good biocompatibility, biodegradability and hydrophilic ability. Moreover, KGM and their derivatives have several importances in the multidirectional research areas such as nutritional, biotechnological and fine chemical fields. In the previous article, we have reviewed the nutritional aspects of KGM covering the various aspects of functional foods, food additives and their derivatives. This review aims at highlighting the diverse biomedical research conducted on KGM in the past ten years, covering therapies for anti-obesity, regulation in lipid metabolism, laxative effect, anti-diabetic, anti-inflammatory, prebiotic to wound dressing applications...
November 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27474722/inclusion-of-konjac-flour-in-the-gestation-diet-changes-the-gut-microbiota-alleviates-oxidative-stress-and-improves-insulin-sensitivity-in-sows
#15
Chengquan Tan, Hongkui Wei, Jiangtao Ao, Guang Long, Jian Peng
UNLABELLED: Although dietary fibers contribute to health and physiology primarily via the fermentative actions of the gut microbiota of the hosts, few studies have focused on how these interactions influence the metabolic status of sows. Here, the effects of inclusion of konjac flour (KF) in a gestation diet on oxidative stress status, insulin sensitivity, and gut microbiota were investigated to elucidate the correlation between the microbiota and metabolic changes in sows. Sows were assigned to either control or 2...
October 1, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27394968/effects-of-dietary-oxidized-konjac-glucomannan-sulfates-okgms-and-acidolysis-oxidized-konjac-glucomannan-a-okgm-on-the-immunity-and-expression-of-immune-related-genes-of-schizothorax-prenanti
#16
Mingrui Chen, Hongjie Wang, Qiuping Yan, Qiaoran Zheng, Min Yang, Zhenzhen Lv, Mei He, Limei Feng, Jiaqi Zhao, Tingting Tang, Yinglong Wu
In the present study, konjac glucomannan (KGM) was degraded by H2O2, and then used trisulfonated sodium amine and HCl, individually, to obtain two kinds of derivatives: oxidized konjac glucomannan sulfates (OKGMS) and acidolysis-oxidized konjac glucomannan (A-OKGM). The effects of two OKGM modified products on the immune parameters and expressions of toll-like receptor 22 (TLR22), myeloid differentiation factor 88 (MyD88) and interferon regulatory factors 7 (IRF7) genes in Schizothorax prenanti were determined...
September 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27392771/physical-stability-and-rheological-properties-of-konjac-glucomannan-ethyl-cellulose-mixed-emulsions
#17
Xuewen Ni, Wenjie Chen, Man Xiao, Kao Wu, Ying Kuang, Harold Corke, Fatang Jiang
Konjac glucomannan (KGM)/ethyl cellulose (EC) could form a stable homogeneous emulsion with appropriate mixing formula. This study aimed to investigate the stable mechanism of KGM/EC emulsion at different mixing ratio through its rheological properties, and droplet size and morphology changes with up to 6days storage time. Though emulsions samples with high KGM content had larger droplet size and worse uniformity at the fresh stage, they appeared to be more stable and droplets growth rate were slow during storage...
July 5, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27374531/the-textural-properties-and-microstructure-of-konjac-glucomannan-tungsten-gels-induced-by-dc-electric-fields
#18
Lixia Wang, Yuanhong Zhuang, Jingliang Li, Jie Pang, Xiangyang Liu
Konjac glucomannan - tungsten (KGM-T) gels were successfully prepared under DC electric fields, in the presence of sodium tungstate. The textural properties and microstructure of the gels were investigated by Texture Analyzer, Rheometer and SEM. Based on the response surface methodology (RSM) results, the optimum conditions for KGM-T gel springiness is 0.32% sodium tungstate concentration, 0.54% KGM concentration, 24.66V voltage and 12.37min treatment time. Under these conditions, the maximum springiness value of KGM-T gel is 1...
December 1, 2016: Food Chemistry
https://www.readbyqxmd.com/read/27372590/utilization-of-konjac-glucomannan-as-a-fat-replacer-in-low-fat-and-skimmed-yogurt
#19
Shuhong Dai, Harold Corke, Nagendra P Shah
Konjac glucomannan (KGM) has been reported to be beneficial to human health, as well as having potential functional properties as a fat replacer in dairy products. In this study, 0.5% KGM solution was added to prepare low-fat (LFKGM) and skimmed (SKKGM) yogurts, and their physicochemical properties were compared with those of full-fat yogurt control (FFC), low-fat yogurt control (LFC), and skimmed yogurt control (SKC). Properties and composition were determined and the microscopic structures of all yogurts were observed during storage at 4°C for 21d...
September 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27324141/efficacy-of-different-fibres-and-flour-mixes-in-south-asian-flatbreads-for-reducing-post-prandial-glucose-responses-in-healthy-adults
#20
Hanny M Boers, Katrina MacAulay, Peter Murray, Jack Seijen Ten Hoorn, Anne-Roos Hoogenraad, Harry P F Peters, Maria A M Vente-Spreeuwenberg, David J Mela
PURPOSE: Type 2 diabetes (T2DM) is increasing, particularly in South-East Asia. Intake of high-glycaemic foods has been positively associated with T2DM, and feasible routes to reduce the glycaemic response to carbohydrate-rich staple foods are needed. The research question was whether different fibre and legume flour mixes in flatbreads lower postprandial glucose (PPG) responses. METHODS: Using a balanced incomplete block design, we tested the inclusion of guar gum (GG), konjac mannan (KM) and chickpea flour (CPF) in 10 combinations (2/4/6 g GG; 2/4 g KM; 15 g CPF, and 10 or 15 g CPF plus 2 or 4 g GG) in 100 g total of a control commercial high-fibre flatbread flour mix ("atta") on PPG in 38 normal-weight adults...
June 21, 2016: European Journal of Nutrition
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