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https://www.readbyqxmd.com/read/28712327/genetic-diversity-and-phylogenetic-relationships-of-seven-amorphophallus-species-in-southwestern-china-revealed-by-chloroplast-dna-sequences
#1
Yong Gao, Si Yin, Huixiao Yang, Lifang Wu, Yuehui Yan
Plants species in the genus Amorphophallus are of great economic importance, as they are the only plants known to produce glucomannan. Although southwestern China has been recognized as one of the origin centres of Amorphophallus, only a few studies assessing its genetic diversity have been reported. To aid in the utilization and conservation of Amorphophallus species, we evaluated the genetic diversity and phylogenetic relationships among seven edible Amorphophallus species using three chloroplast DNA regions (rbcL, trnL and trnK-matK)...
July 15, 2017: Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
https://www.readbyqxmd.com/read/28687934/co-administration-of-a-konjac-based-fibre-blend-and-american-ginseng-panax-quinquefolius-l-on-glycaemic-control-and-serum-lipids-in-type-2-diabetes-a-randomized-controlled-cross-over-clinical-trial
#2
Alexandra L Jenkins, Linda M Morgan, Jacqueline Bishop, Elena Jovanovski, David J A Jenkins, Vladimir Vuksan
PURPOSE: Use of polypharmacy in the treatment of diabetes is the norm; nonetheless, optimal control is often not achieved. Konjac-glucomannan-based fibre blend (KGB) and American ginseng (AG) have individually been shown to improve glycaemia and CVD risk factors in type 2 diabetes. The aim of this study was to determine whether co-administration of KGB and AG could improve diabetes control beyond conventional treatment. METHOD: Thirty-nine participants with type 2 diabetes (6...
July 7, 2017: European Journal of Nutrition
https://www.readbyqxmd.com/read/28656314/suppression-of-gastric-cancer-by-extract-from-the-tuber-of-amorphophallus-konjac-via-induction-of-apoptosis-and-autophagy
#3
Xi Chen, Lin-Qing Yuan, Lin-Jie Li, Yao Lv, Pei-Feng Chen, Lei Pan
The tuber of amorphophallus konjac (TuAK) is an antitumor herb used in traditional Chinese medicine. The present study investigated the inhibitory effect of TuAK against gastric cancer and the underlying mechanisms associated with two programmed cell death pathways, apoptosis and autophagy. TuAK was extracted by organic solvents including ethanol and ligarine. The extract of TuAK, shortened as TuAKe, significantly inhibited the growth of cultured gastric cancer cell lines SGC-7901 and AGS, with IC50 of 35-45 µg/ml...
June 22, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28585167/analysis-of-bacterial-communities-in-rhizosphere-soil-of-continuously-cropped-healthy-and-diseased-konjac
#4
Jinping Wu, Zhenbiao Jiao, Jie Zhou, Fengling Guo, Zili Ding, Zhengming Qiu
The bacterial community and diversity in healthy and diseased konjac rhizosphere soils with different ages of continuous cropping were investigated using next-generation sequencing. The results demonstrated that the number of years of continuous cropping significantly altered soil bacterial community and diversity. Soil bacterial Shannon diversity index and Chao 1 index decreased with the increasing cropping years of konjac. After 1 year of cropping, the soil exhibited the highest bacterial relative abundance and diversity...
July 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28578975/ph-sensitive-drug-delivery-system-based-on-hydrophobic-modified-konjac-glucomannan
#5
Jinling Luan, Kao Wu, Cao Li, Jia Liu, Xuewen Ni, Man Xiao, Yanglin Xu, Ying Kuang, Fatang Jiang
Amphiphilic aliphatic amines grafted konjac glucomannan (KGM-g-AH8, KGM-g-AH12 and KGM-g-AH18) micelles were prepared via a simple two-step synthesis with Schiff's base as the "switch" to achieve intracellular acid-triggered curcumin release. The KGM-g-AH8 self-assembled into spherical nano-micelles (107.6±11.6nm) in an aqueous medium, and presented high curcumin loading capacity as well as good physical stability in 28 days. The in vitro curcumin release behaviors proved the controlled release property and the endosomal/lysosomal pH response of KGM-g-AH8 micelles...
September 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28559619/evaluation-of-different-hydrocolloids-to-improve-dough-rheological-properties-and-bread-quality-of-potato-wheat-flour
#6
Xingli Liu, Taihua Mu, Karim Diego Yamul, Hongnan Sun, Miao Zhang, Jingwang Chen, Marie Laure Fauconnier, Perez Vanina Andrea
The aim of study was to investigate the effect of hydroxylpropylmethylcellulose (HPMC), arabic gum (AG), konjac glucomannan (KG) and apple pectin (AP) at 2% (w/w, potato-wheat flour basis) on the potato-wheat dough (the mass ratio was 1:1) rheological, fermentation and bread making properties. The tan δ of potato-wheat dough was significantly increased upon addition of adding HPMC which was close to wheat dough (0.531). Moreover, dough height during fermentation process was significantly improved on addition of hydrocolloids, with the order of HPMC (23...
May 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28469548/effects-of-dietary-fibers-with-high-water-binding-capacity-and-swelling-capacity-on-gastrointestinal-functions-food-intake-and-body-weight-in-male-rats
#7
Chengquan Tan, Hongkui Wei, Xichen Zhao, Chuanhui Xu, Jian Peng
Objective: The aim of this study was to investigate the effects of supplementation of dietary soluble fibers with high water-binding capacity (WBC) and swelling capacity (SC) on gastrointestinal tract mass, physicochemical properties of digesta, gastrointestinal mean retention time (MRT), body weight, and food intake in male rats. Methods: Thirty-two male Sprague-Dawley rats were randomized to four equal groups and fed the control diet or diet containing 2% konjac flour (KF), pregelatinized waxy maize starch plus guar gum (PWMS+GG), andPWMS plus xanthan gum (PWMS+XG) for three weeks...
2017: Food & Nutrition Research
https://www.readbyqxmd.com/read/28462052/cloning-and-evaluation-of-reference-genes-for-quantitative-real-time-pcr-analysis-in-amorphophallus
#8
Kai Wang, Yi Niu, Qijun Wang, Haili Liu, Yi Jin, Shenglin Zhang
Quantitative real-time reverse transcription PCR (RT-qPCR) has been widely used in the detection and quantification of gene expression levels because of its high accuracy, sensitivity, and reproducibility as well as its large dynamic range. However, the reliability and accuracy of RT-qPCR depends on accurate transcript normalization using stably expressed reference genes. Amorphophallus is a perennial plant with a high content of konjac glucomannan (KGM) in its corm. This crop has been used as a food source and as a traditional medicine for thousands of years...
2017: PeerJ
https://www.readbyqxmd.com/read/28460917/healthy-spanish-salchich%C3%A3-n-enriched-with-encapsulated-n-3-long-chain-fatty-acids-in-konjac-glucomannan-matrix
#9
Jose M Lorenzo, Paulo Eduardo Sichetti Munekata, Mirian Pateiro, Paulo Cezar Bastianello Campagnol, Ruben Domínguez
Reduction of fat content and improving fatty acid composition were the strategies used to reformulate Spanish salchichón with better lipid content by partial substitution of pork backfat by microencapsulated fish oil in konjac glucomannan matrix. For the present study, four different batches were manufactured: control (CO) with 100% of pork backfat and ME25, ME50 and ME75 where the pork backfat was replaced with 25, 50 and 75% by microencapsulated fish oil, respectively. The fat replacement by microencapsulated fish oil was accompanied by a decrease (P<0...
November 2016: Food Research International
https://www.readbyqxmd.com/read/28460335/a-macrophage-activating-injectable-hydrogel-to-sequester-endogenous-growth-factors-for-in-situ-angiogenesis
#10
Yanxian Feng, Qiu Li, Dang Wu, Yiming Niu, Cheng Yang, Lei Dong, Chunming Wang
Biomaterials scaffolds designed for many regenerative applications are expected to support neo-vascularisation, which is now being hampered by two limitations - the instability of exogenous growth factors (GFs) that are delivered to promote angiogenesis; and the loss of extracellular matrix components that bind and stabilise GFs. Here, we report the design and evaluation of an injectable hydrogel system aimed at restoring a GF-binding microenvironment to enhance the pro-angiogenic functions of endogenous GFs...
April 25, 2017: Biomaterials
https://www.readbyqxmd.com/read/28450022/physicochemical-properties-and-cellular-protection-against-oxidation-of-degraded-konjac-glucomannan-prepared-by-%C3%AE-irradiation
#11
Wenjie Jian, Liying Tu, Lanlan Wu, Hejian Xiong, Jie Pang, Yuan-Ming Sun
Konjac glucomannan (KGM) is an important functional polysaccharide in food research. However, unstable dispersibility of KGM inhibits its in-depth study and wide application. In this study, a degraded KGM (100kGy-KGM), which showed excellent dispersibility and specific physicochemical properties, were obtained by γ-irradiation in a dosage of 100kGy. We investigated the protective effect of 100kGy-KGM against H2O2 induced oxidative damage in LO2 cells. Our results demonstrated that pretreatment of LO2 cells with 100kGy-KGM not only significantly increased cellular survivals and activities of GSH-Px and CAT, but also reduced levels of LDH, MDA and intracellular accumulation of ROS...
September 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28401089/rheological-differences-of-waxy-barley-flour-dispersions-mixed-with-various-gums
#12
Chong-Yeon Kim, Byoungseung Yoo
Rheological properties of waxy barley flour (WBF) dispersions mixed with various gums (carboxyl methyl celluleose, guar gum, gum arabic, konjac gum, locust bean gum, tara gum, and xanthan gum) at different gum concentrations were examined in steady and dynamic shear. WBF-gum mixture samples showed a clear trend of shear-thinning behavior and had a non-Newtonian nature with yield stress. Rheological tests indicated that the flow and dynamic rheological parameter (apparent viscosity, consistency index, yield stress, storage modulus, and loss modulus) values of WBF dispersions mixed with gums, except for gum arabic, were significantly higher than those of WBF with no gum, and also increased with an increase in gum concentration...
March 2017: Preventive Nutrition and Food Science
https://www.readbyqxmd.com/read/28372193/effects-of-konjac-glucomannan-on-heat-induced-changes-of-wheat-gluten-structure
#13
Yu Wang, Yiheng Chen, Yun Zhou, Satoru Nirasawa, Eizo Tatsumi, Xiuting Li, Yongqiang Cheng
Effects of konjac glucomannan on the structure of wheat gluten were investigated at variable temperatures in this study. Dynamic oscillatory rheology study showed that konjac glucomannan conferred stiffness on gluten with a higher tan δ data at 25°C and 55°C, but this parameter was lower at 75°C and 95°C. Konjac glucomannan decreased the content of thiol equivalent groups and increased the α-helix/β-sheet content ratio, respectively. The thicker layer of gluten protein with 5% konjac glucomannan was observed by scanning electron microscopy...
August 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28363561/a-kinetic-model-of-the-gelation-of-konjac-glucomannan-induced-by-deacetylation
#14
Ching-Feng Mao, Cheng-Ho Chen
The gelation of konjac glucomannan (KGM) often involves an induction period featured by an initial drop in modulus. The gelation kinetics recorded as a time-dependent modulus change is composed of an initial drop followed by a steady increase in modulus until an equilibrium stage is reached. In this work, the time-dependent modulus curve is described by a kinetic model which incorporates the cascade theory with the kinetic equations consisting of sequential reactions of deacetylation and cross-link formation...
June 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28356275/a-systematic-review-and-meta-analysis-of-randomized-controlled-trials-of-the-effect-of-konjac-glucomannan-a-viscous-soluble-fiber-on-ldl-cholesterol-and-the-new-lipid-targets-non-hdl-cholesterol-and-apolipoprotein-b
#15
REVIEW
Hoang Vi Thanh Ho, Elena Jovanovski, Andreea Zurbau, Sonia Blanco Mejia, John L Sievenpiper, Fei Au-Yeung, Alexandra L Jenkins, Lea Duvnjak, Lawrence Leiter, Vladimir Vuksan
Background: Evidence from randomized controlled trials (RCTs) suggests the consumption of konjac glucomannan (KJM), a viscous soluble fiber, for improving LDL-cholesterol concentrations. It has also been suggested that the cholesterol-lowering potential of KJM may be greater than that of other fibers. However, trials have been relatively scarce and limited in sample size and duration, and the effect estimates have been inconsistent. The effect of KJM on new lipid targets of cardiovascular disease (CVD) risk is also unknown...
May 2017: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28330208/purification-and-characterization-of-%C3%AE-mannanase-from-aspergillus-terreus-and-its-applicability-in-depolymerization-of-mannans-and-saccharification-of-lignocellulosic-biomass
#16
Hemant Soni, Hemant Kumar Rawat, Brett I Pletschke, Naveen Kango
Aspergillus terreus FBCC 1369 was grown in solid-state culture under statistically optimized conditions. β-Mannanase was purified to apparent homogeneity by ultrafiltration, anion exchange and gel filtration chromatography. A purification factor of 10.3-fold was achieved, with the purified enzyme exhibiting specific activity of 53 U/mg protein. The purified β-mannanase was optimally active at pH 7.0 and 70 °C and displayed stability over a broad pH range of 4.0-8.0 and a 30 min half-life at 80 °C. The molecular weight of β-mannanase was calculated as ~49 kDa by SDS-PAGE...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28248105/manufacture-and-properties-of-glucomannans-and-glucomannooligosaccharides-derived-from-konjac-and-other-sources
#17
REVIEW
Belén Gómez, Beatriz Míguez, Remedios Yáñez, José L Alonso
Glucomannans (GM) are polymers that can be found in natural resources, such as tubers, bulbs, roots, and both hard- and softwoods. In fact, mannan-based polysaccharides represent the largest hemicellulose fraction in softwoods. In addition to their structural functions and their role as energy reserve, they have been assessed for their healthy applications, including their role as new source of prebiotics. This paper summarizes the scientific literature regarding the manufacture and functional properties of GM and their hydrolysis products with a special focus on their prebiotic activity...
March 15, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28224896/carboxylmethyl-konjac-glucomannan-conjugated-polydopamine-composites-for-pb-ii-removal
#18
Pan-Pan Chen, Hong-Ping Zhang, Jianping Ding, Xiao-Yan Lin, Xiong Lu, Changsheng Liu, Youhong Tang
Carboxylmethyl konjac glucomannan conjugated polydopamine (CMKGM-PDA) composite was successfully prepared using a cost-effective method. CMKGM-PDA exhibited excellent adsorption performance for the removal of Pb(II) and could be a convenient agent for recovery. The Langmuir linear model was suitable for describing the adsorption process of Pb(II). The maximum adsorption capacity was 95.24mgg(-1) at 298K, showing a high absorption capacity in comparison to similar absorbents. The pseudo-second order equation and intra-particle diffusion model exhibited good correlation with the adsorption kinetic...
April 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28220352/purification-characterization-and-overexpression-of-an-endo-1-4-%C3%AE-mannanase-from-thermotolerant-bacillus-sp-swu60
#19
Weeranuch Seesom, Polphet Thongket, Tomohiro Yamamoto, Shigeo Takenaka, Tatsuji Sakamoto, Wasana Sukhumsirichart
Endo-β-1,4-mannanases are important catalytic agents in several industries. The enzymes randomly cleave the β-1,4-linkage in the mannan backbone and release short β-1,4-mannooligosaccharides and mannose. In the present study, mannanase (ManS2) from thermotolerant Bacillus sp. SWU60 was purified, characterized, and its gene was cloned and overexpressed in Escherichia coli. ManS2 was purified from culture filtrate (300 ml) by using hydrophobic, ion-exchange, and size-exclusive liquid chromatography. The apparent molecular mass was 38 kDa...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28169529/deacetylated-konjac-glucomannan-is-less-effective-in-reducing-dietary-induced-hyperlipidemia-and-hepatic-steatosis-in-c57bl-6-mice
#20
COMPARATIVE STUDY
Mei-Ying Li, Guan-Ping Feng, Hong Wang, Rui-Li Yang, Zhenlin Xu, Yuan-Ming Sun
Konjac gel foods that mainly consist of deacetylated konjac glucomannan (Da-KGM) are considered to have the same health benefits as native konjac glucomannan (KGM); however, no definitive data support this notion. The objective of this study was to compare the effects of Da-KGM and KGM on the hyperlipidemia and liver steatosis induced by high-fat diet feeding and to investigate the underlying molecular mechanisms. C57BL/6 mice were fed (1) normal chow diet, (2) high-fat diet, (3) HFD with KGM, or (4) HFD with Da-KGM for 10 weeks...
March 1, 2017: Journal of Agricultural and Food Chemistry
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