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https://www.readbyqxmd.com/read/28169529/deacetylated-konjac-glucomannan-is-less-effective-in-reducing-dietary-induced-hyperlipidemia-and-hepatic-steatosis-in-c57bl-6-mice
#1
Meiying Li, Guanping Feng, Hong Wang, Ruili Yang, Zhenlin Xu, Yuanming Sun
Konjac gel foods that mainly consist of deacetylated konjac glucomannan (Da-KGM) are considered to have the same health benefits as native konjac glucomannan (KGM); however, no definitive data support this notion. The objective of this study was to compare the effects of Da-KGM and KGM on the hyperlipidemia and liver steatosis induced by high-fat diet feeding and to investigate the underlying molecular mechanisms. C57BL/6 mice were fed (1) normal chow diet; (2) high-fat diet; (3) HFD with KGM; (4) HFD with Da-KGM for 10 weeks...
February 7, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28130129/heparin-mimetic-polyurethane-hydrogels-with-anticoagulant-tunable-mechanical-property-and-controllable-drug-releasing-behavior
#2
Yuan Chen, Rui Wang, Yonghui Wang, Weifeng Zhao, Shudong Sun, Changsheng Zhao
In the present study, novel heparin-mimetic polyurethane hydrogels were prepared by introducing chemical crosslinked sulfated konjac glucomannan (SKGM). Scanning electron microscopy (SEM) results indicated that the introduction of SKGM and the increase of the molecular weight of diol segments could enlarge the pore sizes of the hydrogels. The swelling behavior corresponded with the SEM results, and the hydrogels could absorb more water after the modification. The modification also led to an improvement in the mechanical property...
January 24, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28105485/biochemical-characterization-of-thermostable-%C3%AE-1-4-mannanase-belonging-to-the-glycoside-hydrolase-family-134-from-aspergillus-oryzae
#3
Kiyota Sakai, Mai Mochizuki, Miyuki Yamada, Yuta Shinzawa, Miho Minezawa, Saran Kimoto, Shunsuke Murata, Yuhei Kaneko, Saaya Ishihara, Sadanari Jindou, Tetsuo Kobayashi, Masashi Kato, Motoyuki Shimizu
A β-1,4-mannanase, termed AoMan134A, that belongs to the GH 134 family was identified in the filamentous fungus Aspergillus oryzae. Recombinant AoMan134A was expressed in Pichia pastoris, and the purified enzyme produced mannobiose, mannotriose, mannotetraose, and mannopentaose from galactose-free β-mannan, with mannotriose being the predominant reaction product. The catalytic efficiency (k cat/K m ) of AoMan134A was 6.8-fold higher toward galactomannan from locust bean gum, than toward galactomannan from guar gum, but similar toward galactomannan from locust bean gum and glucomannan from konjac flour...
January 19, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28038754/molecular-weight-distribution-and-fermentation-of-mechanically-pre-treated-konjac-enzymatic-hydrolysates
#4
Jun Yang, Natale Vittori, Weiwei Wang, Yong-Cheng Shi, Jennifer L Hoeflinger, Michael J Miller, Yang Pan
There is interest in novel fibers as potential prebiotics for new and reformulated food products. Two konjac glucomannan (KGM) hydrolysates were developed by enzymatic hydrolysis with (KGMH I) or without (KGMH II) mechanical shear pre-treatment. These were characterized and evaluated as fermentation substrates using five lactobacilli and three bifidobacteria. Enzymatic treatment of native KGM reduced the average molecular weights of supernatant and pellet by ∼3-fold. Additional mechanical shear pre-treatment further reduced supernatant and pellet molecular weights by 5% and 35%, respectively...
March 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28017981/production-and-storage-stability-of-formulated-chicken-nuggets-using-konjac-flour-and-shiitake-mushrooms
#5
Adisak Akesowan
Formulated chicken nuggets which are low in fat and, high in dietary fiber and free from phosphate were developed by adding various levels of a konjac flour/xanthan gum (KF/XG) (3:1) mixture (0.2-1.5 %, w/w) and shiitake powder (SP) (1-4 %, w/w). A central composite rotatable design was used to investigate the influence of variables on the physical and sensory properties of nuggets and to optimize the formulated nugget formulation. The addition of the KF/XG mixture and SP was effective in improving nugget firmness and increasing hedonic scores for color, taste, flavor and overall acceptability...
October 2016: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/27988000/co-loading-and-intestine-specific-delivery-of-multiple-antioxidants-in-ph-responsive-microspheres-based-on-tempo-oxidized-polysaccharides
#6
Mengxuan Shi, Jie Bai, Liyun Zhao, Xinrui Yu, Jingjing Liang, Ying Liu, Willem Nord, Yuan Li
In this study, pH-responsive microspheres loaded with multiple antioxidants were developed for intestine-specific delivery and exhibited synergistic activity. They consist of chitosan (CS)-coated microspheres made of TEMPO-oxidized Konjac glucomannan (OKGM) polymers, of which the carboxyl (COO(-)) groups are cross-linked via ferric ions (Fe(3+)), allowing the hydrophobic (β-carotene) and hydrophilic (anthocyanins) antioxidants to be simultaneously incorporated. CLSM images showed successful co-encapsulation of β-carotene and anthocyanins...
February 10, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27868067/purification-and-characterization-of-a-thermostable-%C3%AE-mannanase-from-bacillus-subtilis-be-91-potential-application-in-inflammatory-diseases
#7
Lifeng Cheng, Shengwen Duan, Xiangyuan Feng, Ke Zheng, Qi Yang, Zhengchu Liu
β-mannanase has shown compelling biological functions because of its regulatory roles in metabolism, inflammation, and oxidation. This study separated and purified the β-mannanase from Bacillus subtilis BE-91, which is a powerful hemicellulose-degrading bacterium using a "two-step" method comprising ultrafiltration and gel chromatography. The purified β-mannanase (about 28.2 kDa) showed high specific activity (79, 859.2 IU/mg). The optimum temperature and pH were 65°C and 6.0, respectively. Moreover, the enzyme was highly stable at temperatures up to 70°C and pH 4...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27842852/characteristics-of-glucomannan-isolated-from-fresh-tuber-of-porang-amorphophallus-muelleri-blume
#8
Anny Yanuriati, Djagal Wiseso Marseno, Rochmadi, Eni Harmayani
Porang is a potential source of glucomannan. This research objective was to find a direct glucomannan isolation method from fresh porang corm to produce high purity glucomannan. Two isolation methods were performed. In first method, sample was water dissolved using Al2(SO4)3 as flocculant for 15 (AA15) or 30 (AA30) minutes with purification. In second method, sample was repeatedly milled using ethanol as solvent and filtered for 5 (EtOH5) or 7 (EtOH7) times without purification. The characteristics of obtained glucomannan were compared to those of commercial porang flour (CPF) and purified konjac glucomannan (PKG)...
January 20, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27835835/effect-of-natural-antioxidants-in-spanish-salchich%C3%A3-n-elaborated-with-encapsulated-n-3-long-chain-fatty-acids-in-konjac-glucomannan-matrix
#9
P E S Munekata, R Domínguez, D Franco, R Bermúdez, M A Trindade, Jose M Lorenzo
The effect of natural antioxidants on physicochemical properties, lipid and protein oxidation, volatile compounds and free fatty acids (FFA) were determined in Spanish salchichón enriched with n-3 fatty acids encapsulated and stabilized in konjac matrix. Phenolic compounds of beer residue extract (BRE), chestnut leaves extract (CLE) and peanut skin extract (PSE) were also identified and quantified. Five batches of salchichón were prepared: control (CON, without antioxidants), butylated hydroxytoluene (BHT), BRE, CLE and PSE...
February 2017: Meat Science
https://www.readbyqxmd.com/read/27819518/x-ray-induced-mutation-of-bacillus-sp-mr10-for-manno-oligosaccharides-production-from-copra-meal
#10
Siriporn Chaikaew, Apinun Kanpiengjai, Jenjira Intatep, Kridsada Unban, Pairote Wongputtisin, Goro Takata, Chartchai Khanongnuch
The present study demonstrates the effectiveness of X-ray radiation in strain improvement for defective lipase production by Bacillus sp. MR10 for further application in the fermentative production of manno-oligosaccharides (MOS) from agricultural by-product, defatted copra meal (DCM). The mutants obtained were screened based on their defective lipase activity together with their β-mannanase production performance. Among 10 selected mutants, the strain M7 was the highest promising mutant regarding the smallest lipase activity (0...
November 7, 2016: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/27773606/engineering-a-family-27-carbohydrate-binding-module-into-an-aspergillus-usamii-%C3%AE-mannanase-to-perfect-its-enzymatic-properties
#11
Jianfang Li, Chunjuan Wang, Die Hu, Fengjiao Yuan, Xueqing Li, Shihan Tang, Minchen Wu
A family 27 carbohydrate-binding module of a Thermotoga maritima β-mannanase (TmCBM27) was chosen from the carbohydrate-active enzyme database by computer-aided design, possessing the lowest binding free energy with mannopentaose. To improve the enzymatic properties of a glycoside hydrolase family 5 β-mannanase from Aspergillus usamii (AuMan5A), two fusion β-mannanases, AuMan5A-F-M and AuMan5A-R-M, were designed by fusing a TmCBM27 into its C-terminus linked with a flexible peptide F (GGGGS)3 and rigid peptide R (EAAAK)3...
October 20, 2016: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/27771411/da-kgm-based-go-reinforced-fmbo-loaded-aerogels-for-efficient-arsenic-removal-in-aqueous-solution
#12
Shuxin Ye, Weiping Jin, Qing Huang, Ying Hu, Yan Li, Jing Li, Bin Li
Composites based on deacetylated konjac glucomannan (Da-KGM) and graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water. Da-KGM, which was used as supporting composite matrix here, were firstly treated with GO and loaded FMBO. The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM)...
January 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27709705/undisclosed-presence-of-methylisothiazolinone-in-100-natural-konjac%C3%A2-sponge
#13
Jakob T Madsen, Klaus E Andersen, Dorte T Nielsen, Lone Hvid, Rime B El-Houri, Lars P Christensen
No abstract text is available yet for this article.
November 2016: Contact Dermatitis
https://www.readbyqxmd.com/read/27706095/soluble-fiber-with-high-water-binding-capacity-swelling-capacity-and-fermentability-reduces-food-intake-by-promoting-satiety-rather-than-satiation-in-rats
#14
Chengquan Tan, Hongkui Wei, Xichen Zhao, Chuanhui Xu, Yuanfei Zhou, Jian Peng
To understand whether soluble fiber (SF) with high water-binding capacity (WBC), swelling capacity (SC) and fermentability reduces food intake and whether it does so by promoting satiety or satiation or both, we investigated the effects of different SFs with these properties on the food intake in rats. Thirty-two male Sprague-Dawley rats were randomized to four equal groups and fed the control diet or diet containing 2% konjac flour (KF), pregelatinized waxy maize starch (PWMS) plus guar gum (PG), and PWMS starch plus xanthan gum (PX) for three weeks, with the measured values of SF, WBC, and SC in the four diets following the order of PG > KF > PX > control...
October 2, 2016: Nutrients
https://www.readbyqxmd.com/read/27702490/genome-mining-and-motif-truncation-of-glycoside-hydrolase-family-5-endo-%C3%AE-1-4-mannanase-encoded-by-aspergillus-oryzae-rib40-for-potential-konjac-flour-hydrolysis-or-feed-additive
#15
Cun-Duo Tang, Hong-Ling Shi, Qing-Hai Tang, Jun-Shi Zhou, Lun-Guang Yao, Zhu-Jin Jiao, Yun-Chao Kan
Two novel glycosyl hydrolase family 5 (GH5) β-mannanases (AoMan5A and AoMan5B) were identified from Aspergillus oryzae RIB40 by genome mining. The AoMan5A contains a predicted family 1 carbohydrate binding module (CBM-1), located at its N-terminal. The AoMan5A, AoMan5B and truncated mutant AoMan5AΔCL (truncating the N-terminal CBM and linker of AoMan5A) were expressed retaining the N-terminus of the native protein in Pichia pastoris GS115 by pPIC9K(M). The specific enzyme activity of the purified reAoMan5A, reAoMan5B and reAoMan5AΔCL towards locust bean gum at pH 3...
November 2016: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/27694039/the-effects-of-konjac-oligosaccharide-on-tnbs-induced-colitis-in-rats
#16
Ruixue Liu, Yongchao Li, Bo Zhang
The purpose of the study was to assess the effects and the protective mechanism of konjac oligosaccharide (KOS) on the ulcerative colitis (UC) model induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) in rats. KOS (1.0 and 4.0g/kg/day) was administered for 14days after the induction of colitis with TNBS. The status of the rats was assessed by morphological and biochemical methods. The effect of KOS on the colonic microflora was also assessed by studying the bacteria profile and short chain fatty acids (SCFAs) production in feces by standard culture techniques and gas chromatography, respectively...
November 2016: International Immunopharmacology
https://www.readbyqxmd.com/read/27615677/ultralight-biomass-derived-carbonaceous-nanofibrous-aerogels-with-superelasticity-and-high-pressure-sensitivity
#17
Yang Si, Xueqin Wang, Chengcheng Yan, Liu Yang, Jianyong Yu, Bin Ding
Superelastic and pressure-sensitive carbonaceous nanofibrous aerogels with a honeycomb-like structure are fabricated through the combination of sustainable konjac glucomannan biomass and flexible SiO2 nanofibers. The aerogels can detect dynamic pressure with a wide pressure range and high sensitivity, which enables real pressure signals such as human blood pulses to be monitored in real time and in situ.
September 12, 2016: Advanced Materials
https://www.readbyqxmd.com/read/27521845/the-control-of-ice-crystal-growth-and-effect-on-porous-structure-of-konjac-glucomannan-based-aerogels
#18
Xuewen Ni, Fan Ke, Man Xiao, Kao Wu, Ying Kuang, Harold Corke, Fatang Jiang
Konjac glucomannan (KGM)-based aerogels were prepared using a combination of sol-gel and freeze-drying methods. Preparation conditions were chosen to control ice crystal growth and aerogel structure formation. The ice crystals formed during pre-freezing were observed by low temperature polarizing microscopy, and images of aerogel pores were obtained by scanning electron microscopy. The size of ice crystals were calculated and size distribution maps were drawn, and similarly for aerogel pores. Results showed that ice crystal growth and aerogel pore sizes may be controlled by varying pre-freezing temperatures, KGM concentration and glyceryl monostearate concentration...
November 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27492560/kgm-based-magnetic-carbon-aerogels-matrix-for-the-uptake-of-methylene-blue-and-methyl-orange
#19
Shuxin Ye, Weiping Jin, Qing Huang, Ying Hu, Yan Li, Bin Li
In this study, the preparation of magnetic Fe and Mn oxides (Mag-FMBO) loaded carbon aerogels (CA) based on konjac glucomannan (KGM) and their performance for dyes adsorption were investigated. The prepared magnetic carbon aerogels (Mag-CA) materials were characterized by various methods, including BET surface area analysis, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and vibrating sample magnetometer (VSM). What's more, the Mag-CA materials were used as adsorbents to remove dyes [anionic methyl orange (MO) and cationic methylene blue (MB)] from aqueous solutions...
November 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27481345/konjac-glucomannan-a-promising-polysaccharide-of-amorphophallus-konjac-k-koch-in-health-care
#20
Sudhanshu S Behera, Ramesh C Ray
In recent year, konjac glucomannan (KGM) has attracted more attention due to its non-harmful and non-toxic properties, good biocompatibility, biodegradability and hydrophilic ability. Moreover, KGM and their derivatives have several importances in the multidirectional research areas such as nutritional, biotechnological and fine chemical fields. In the previous article, we have reviewed the nutritional aspects of KGM covering the various aspects of functional foods, food additives and their derivatives. This review aims at highlighting the diverse biomedical research conducted on KGM in the past ten years, covering therapies for anti-obesity, regulation in lipid metabolism, laxative effect, anti-diabetic, anti-inflammatory, prebiotic to wound dressing applications...
November 2016: International Journal of Biological Macromolecules
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