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https://www.readbyqxmd.com/read/28401089/rheological-differences-of-waxy-barley-flour-dispersions-mixed-with-various-gums
#1
Chong-Yeon Kim, Byoungseung Yoo
Rheological properties of waxy barley flour (WBF) dispersions mixed with various gums (carboxyl methyl celluleose, guar gum, gum arabic, konjac gum, locust bean gum, tara gum, and xanthan gum) at different gum concentrations were examined in steady and dynamic shear. WBF-gum mixture samples showed a clear trend of shear-thinning behavior and had a non-Newtonian nature with yield stress. Rheological tests indicated that the flow and dynamic rheological parameter (apparent viscosity, consistency index, yield stress, storage modulus, and loss modulus) values of WBF dispersions mixed with gums, except for gum arabic, were significantly higher than those of WBF with no gum, and also increased with an increase in gum concentration...
March 2017: Preventive Nutrition and Food Science
https://www.readbyqxmd.com/read/28372193/effects-of-konjac-glucomannan-on-heat-induced-changes-of-wheat-gluten-structure
#2
Yu Wang, Yiheng Chen, Yun Zhou, Satoru Nirasawa, Eizo Tatsumi, Xiuting Li, Yongqiang Cheng
Effects of konjac glucomannan on the structure of wheat gluten were investigated at variable temperatures in this study. Dynamic oscillatory rheology study showed that konjac glucomannan conferred stiffness on gluten with a higher tan δ data at 25°C and 55°C, but this parameter was lower at 75°C and 95°C. Konjac glucomannan decreased the content of thiol equivalent groups and increased the α-helix/β-sheet content ratio, respectively. The thicker layer of gluten protein with 5% konjac glucomannan was observed by scanning electron microscopy...
August 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28363561/a-kinetic-model-of-the-gelation-of-konjac-glucomannan-induced-by-deacetylation
#3
Ching-Feng Mao, Cheng-Ho Chen
The gelation of konjac glucomannan (KGM) often involves an induction period featured by an initial drop in modulus. The gelation kinetics recorded as a time-dependent modulus change is composed of an initial drop followed by a steady increase in modulus until an equilibrium stage is reached. In this work, the time-dependent modulus curve is described by a kinetic model which incorporates the cascade theory with the kinetic equations consisting of sequential reactions of deacetylation and cross-link formation...
June 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28356275/a-systematic-review-and-meta-analysis-of-randomized-controlled-trials-of-the-effect-of-konjac-glucomannan-a-viscous-soluble-fiber-on-ldl-cholesterol-and-the-new-lipid-targets-non-hdl-cholesterol-and-apolipoprotein-b
#4
Hoang Vi Thanh Ho, Elena Jovanovski, Andreea Zurbau, Sonia Blanco Mejia, John L Sievenpiper, Fei Au-Yeung, Alexandra L Jenkins, Lea Duvnjak, Lawrence Leiter, Vladimir Vuksan
Background: Evidence from randomized controlled trials (RCTs) suggests the consumption of konjac glucomannan (KJM), a viscous soluble fiber, for improving LDL-cholesterol concentrations. It has also been suggested that the cholesterol-lowering potential of KJM may be greater than that of other fibers. However, trials have been relatively scarce and limited in sample size and duration, and the effect estimates have been inconsistent. The effect of KJM on new lipid targets of cardiovascular disease (CVD) risk is also unknown...
March 29, 2017: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28330208/purification-and-characterization-of-%C3%AE-mannanase-from-aspergillus-terreus-and-its-applicability-in-depolymerization-of-mannans-and-saccharification-of-lignocellulosic-biomass
#5
Hemant Soni, Hemant Kumar Rawat, Brett I Pletschke, Naveen Kango
Aspergillus terreus FBCC 1369 was grown in solid-state culture under statistically optimized conditions. β-Mannanase was purified to apparent homogeneity by ultrafiltration, anion exchange and gel filtration chromatography. A purification factor of 10.3-fold was achieved, with the purified enzyme exhibiting specific activity of 53 U/mg protein. The purified β-mannanase was optimally active at pH 7.0 and 70 °C and displayed stability over a broad pH range of 4.0-8.0 and a 30 min half-life at 80 °C. The molecular weight of β-mannanase was calculated as ~49 kDa by SDS-PAGE...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28248105/manufacture-and-properties-of-glucomannans-and-glucomannooligosaccharides-derived-from-konjac-and-other-sources
#6
Belén Gómez, Beatriz Míguez, Remedios Yáñez, José L Alonso
Glucomannans (GM) are polymers that can be found in natural resources, such as tubers, bulbs, roots, and both hard- and softwoods. In fact, mannan-based polysaccharides represent the largest hemicellulose fraction in softwoods. In addition to their structural functions and their role as energy reserve, they have been assessed for their healthy applications, including their role as new source of prebiotics. This paper summarizes the scientific literature regarding the manufacture and functional properties of GM and their hydrolysis products with a special focus on their prebiotic activity...
March 1, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28224896/carboxylmethyl-konjac-glucomannan-conjugated-polydopamine-composites-for-pb-ii-removal
#7
Pan-Pan Chen, Hong-Ping Zhang, Jianping Ding, Xiao-Yan Lin, Xiong Lu, Changsheng Liu, Youhong Tang
Carboxylmethyl konjac glucomannan conjugated polydopamine (CMKGM-PDA) composite was successfully prepared using a cost-effective method. CMKGM-PDA exhibited excellent adsorption performance for the removal of Pb(II) and could be a convenient agent for recovery. The Langmuir linear model was suitable for describing the adsorption process of Pb(II). The maximum adsorption capacity was 95.24mgg(-1) at 298K, showing a high absorption capacity in comparison to similar absorbents. The pseudo-second order equation and intra-particle diffusion model exhibited good correlation with the adsorption kinetic...
April 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28220352/purification-characterization-and-overexpression-of-an-endo-1-4-%C3%AE-mannanase-from-thermotolerant-bacillus-sp-swu60
#8
Weeranuch Seesom, Polphet Thongket, Tomohiro Yamamoto, Shigeo Takenaka, Tatsuji Sakamoto, Wasana Sukhumsirichart
Endo-β-1,4-mannanases are important catalytic agents in several industries. The enzymes randomly cleave the β-1,4-linkage in the mannan backbone and release short β-1,4-mannooligosaccharides and mannose. In the present study, mannanase (ManS2) from thermotolerant Bacillus sp. SWU60 was purified, characterized, and its gene was cloned and overexpressed in Escherichia coli. ManS2 was purified from culture filtrate (300 ml) by using hydrophobic, ion-exchange, and size-exclusive liquid chromatography. The apparent molecular mass was 38 kDa...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28169529/deacetylated-konjac-glucomannan-is-less-effective-in-reducing-dietary-induced-hyperlipidemia-and-hepatic-steatosis-in-c57bl-6-mice
#9
Mei-Ying Li, Guan-Ping Feng, Hong Wang, Rui-Li Yang, Zhenlin Xu, Yuan-Ming Sun
Konjac gel foods that mainly consist of deacetylated konjac glucomannan (Da-KGM) are considered to have the same health benefits as native konjac glucomannan (KGM); however, no definitive data support this notion. The objective of this study was to compare the effects of Da-KGM and KGM on the hyperlipidemia and liver steatosis induced by high-fat diet feeding and to investigate the underlying molecular mechanisms. C57BL/6 mice were fed (1) normal chow diet, (2) high-fat diet, (3) HFD with KGM, or (4) HFD with Da-KGM for 10 weeks...
February 17, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28130129/heparin-mimetic-polyurethane-hydrogels-with-anticoagulant-tunable-mechanical-property-and-controllable-drug-releasing-behavior
#10
Yuan Chen, Rui Wang, Yonghui Wang, Weifeng Zhao, Shudong Sun, Changsheng Zhao
In the present study, novel heparin-mimetic polyurethane hydrogels were prepared by introducing chemical crosslinked sulfated konjac glucomannan (SKGM). Scanning electron microscopy (SEM) results indicated that the introduction of SKGM and the increase of the molecular weight of diol segments could enlarge the pore sizes of the hydrogels. The swelling behavior corresponded with the SEM results, and the hydrogels could absorb more water after the modification. The modification also led to an improvement in the mechanical property...
January 24, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28105485/biochemical-characterization-of-thermostable-%C3%AE-1-4-mannanase-belonging-to-the-glycoside-hydrolase-family-134-from-aspergillus-oryzae
#11
Kiyota Sakai, Mai Mochizuki, Miyuki Yamada, Yuta Shinzawa, Miho Minezawa, Saran Kimoto, Shunsuke Murata, Yuhei Kaneko, Saaya Ishihara, Sadanari Jindou, Tetsuo Kobayashi, Masashi Kato, Motoyuki Shimizu
A β-1,4-mannanase, termed AoMan134A, that belongs to the GH 134 family was identified in the filamentous fungus Aspergillus oryzae. Recombinant AoMan134A was expressed in Pichia pastoris, and the purified enzyme produced mannobiose, mannotriose, mannotetraose, and mannopentaose from galactose-free β-mannan, with mannotriose being the predominant reaction product. The catalytic efficiency (k cat/K m ) of AoMan134A was 6.8-fold higher toward galactomannan from locust bean gum, than toward galactomannan from guar gum, but similar toward galactomannan from locust bean gum and glucomannan from konjac flour...
January 19, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28038754/molecular-weight-distribution-and-fermentation-of-mechanically-pre-treated-konjac-enzymatic-hydrolysates
#12
Jun Yang, Natale Vittori, Weiwei Wang, Yong-Cheng Shi, Jennifer L Hoeflinger, Michael J Miller, Yang Pan
There is interest in novel fibers as potential prebiotics for new and reformulated food products. Two konjac glucomannan (KGM) hydrolysates were developed by enzymatic hydrolysis with (KGMH I) or without (KGMH II) mechanical shear pre-treatment. These were characterized and evaluated as fermentation substrates using five lactobacilli and three bifidobacteria. Enzymatic treatment of native KGM reduced the average molecular weights of supernatant and pellet by ∼3-fold. Additional mechanical shear pre-treatment further reduced supernatant and pellet molecular weights by 5% and 35%, respectively...
March 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28017981/production-and-storage-stability-of-formulated-chicken-nuggets-using-konjac-flour-and-shiitake-mushrooms
#13
Adisak Akesowan
Formulated chicken nuggets which are low in fat and, high in dietary fiber and free from phosphate were developed by adding various levels of a konjac flour/xanthan gum (KF/XG) (3:1) mixture (0.2-1.5 %, w/w) and shiitake powder (SP) (1-4 %, w/w). A central composite rotatable design was used to investigate the influence of variables on the physical and sensory properties of nuggets and to optimize the formulated nugget formulation. The addition of the KF/XG mixture and SP was effective in improving nugget firmness and increasing hedonic scores for color, taste, flavor and overall acceptability...
October 2016: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/27988000/co-loading-and-intestine-specific-delivery-of-multiple-antioxidants-in-ph-responsive-microspheres-based-on-tempo-oxidized-polysaccharides
#14
Mengxuan Shi, Jie Bai, Liyun Zhao, Xinrui Yu, Jingjing Liang, Ying Liu, Willem Nord, Yuan Li
In this study, pH-responsive microspheres loaded with multiple antioxidants were developed for intestine-specific delivery and exhibited synergistic activity. They consist of chitosan (CS)-coated microspheres made of TEMPO-oxidized Konjac glucomannan (OKGM) polymers, of which the carboxyl (COO(-)) groups are cross-linked via ferric ions (Fe(3+)), allowing the hydrophobic (β-carotene) and hydrophilic (anthocyanins) antioxidants to be simultaneously incorporated. CLSM images showed successful co-encapsulation of β-carotene and anthocyanins...
February 10, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27868067/purification-and-characterization-of-a-thermostable-%C3%AE-mannanase-from-bacillus-subtilis-be-91-potential-application-in-inflammatory-diseases
#15
Lifeng Cheng, Shengwen Duan, Xiangyuan Feng, Ke Zheng, Qi Yang, Zhengchu Liu
β-mannanase has shown compelling biological functions because of its regulatory roles in metabolism, inflammation, and oxidation. This study separated and purified the β-mannanase from Bacillus subtilis BE-91, which is a powerful hemicellulose-degrading bacterium using a "two-step" method comprising ultrafiltration and gel chromatography. The purified β-mannanase (about 28.2 kDa) showed high specific activity (79, 859.2 IU/mg). The optimum temperature and pH were 65°C and 6.0, respectively. Moreover, the enzyme was highly stable at temperatures up to 70°C and pH 4...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27842852/characteristics-of-glucomannan-isolated-from-fresh-tuber-of-porang-amorphophallus-muelleri-blume
#16
Anny Yanuriati, Djagal Wiseso Marseno, Rochmadi, Eni Harmayani
Porang is a potential source of glucomannan. This research objective was to find a direct glucomannan isolation method from fresh porang corm to produce high purity glucomannan. Two isolation methods were performed. In first method, sample was water dissolved using Al2(SO4)3 as flocculant for 15 (AA15) or 30 (AA30) minutes with purification. In second method, sample was repeatedly milled using ethanol as solvent and filtered for 5 (EtOH5) or 7 (EtOH7) times without purification. The characteristics of obtained glucomannan were compared to those of commercial porang flour (CPF) and purified konjac glucomannan (PKG)...
January 20, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27835835/effect-of-natural-antioxidants-in-spanish-salchich%C3%A3-n-elaborated-with-encapsulated-n-3-long-chain-fatty-acids-in-konjac-glucomannan-matrix
#17
P E S Munekata, R Domínguez, D Franco, R Bermúdez, M A Trindade, Jose M Lorenzo
The effect of natural antioxidants on physicochemical properties, lipid and protein oxidation, volatile compounds and free fatty acids (FFA) were determined in Spanish salchichón enriched with n-3 fatty acids encapsulated and stabilized in konjac matrix. Phenolic compounds of beer residue extract (BRE), chestnut leaves extract (CLE) and peanut skin extract (PSE) were also identified and quantified. Five batches of salchichón were prepared: control (CON, without antioxidants), butylated hydroxytoluene (BHT), BRE, CLE and PSE...
February 2017: Meat Science
https://www.readbyqxmd.com/read/27819518/x-ray-induced-mutation-of-bacillus-sp-mr10-for-manno-oligosaccharides-production-from-copra-meal
#18
Siriporn Chaikaew, Apinun Kanpiengjai, Jenjira Intatep, Kridsada Unban, Pairote Wongputtisin, Goro Takata, Chartchai Khanongnuch
The present study demonstrates the effectiveness of X-ray radiation in strain improvement for defective lipase production by Bacillus sp. MR10 for further application in the fermentative production of manno-oligosaccharides (MOS) from agricultural by-product, defatted copra meal (DCM). The mutants obtained were screened based on their defective lipase activity together with their β-mannanase production performance. Among 10 selected mutants, the strain M7 was the highest promising mutant regarding the smallest lipase activity (0...
April 21, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/27773606/engineering-a-family-27-carbohydrate-binding-module-into-an-aspergillus-usamii-%C3%AE-mannanase-to-perfect-its-enzymatic-properties
#19
Jianfang Li, Chunjuan Wang, Die Hu, Fengjiao Yuan, Xueqing Li, Shihan Tang, Minchen Wu
A family 27 carbohydrate-binding module of a Thermotoga maritima β-mannanase (TmCBM27) was chosen from the carbohydrate-active enzyme database by computer-aided design, possessing the lowest binding free energy with mannopentaose. To improve the enzymatic properties of a glycoside hydrolase family 5 β-mannanase from Aspergillus usamii (AuMan5A), two fusion β-mannanases, AuMan5A-F-M and AuMan5A-R-M, were designed by fusing a TmCBM27 into its C-terminus linked with a flexible peptide F (GGGGS)3 and rigid peptide R (EAAAK)3...
March 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/27771411/da-kgm-based-go-reinforced-fmbo-loaded-aerogels-for-efficient-arsenic-removal-in-aqueous-solution
#20
Shuxin Ye, Weiping Jin, Qing Huang, Ying Hu, Yan Li, Jing Li, Bin Li
Composites based on deacetylated konjac glucomannan (Da-KGM) and graphene oxide (GO) aerogels with iron and manganese oxides (FMBO) for effective removal of arsenic from contaminated water. Da-KGM, which was used as supporting composite matrix here, were firstly treated with GO and loaded FMBO. The obtained Da-KGM/GO/FMBO composite aerogels were characterized by compression test, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM)...
January 2017: International Journal of Biological Macromolecules
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