Lucas G Dent, Nathan Curry, Hugh Sparks, Vicky Bousgouni, Vincent Maioli, Sunil Kumar, Ian Munro, Francesca Butera, Ian Jones, Mar Arias-Garcia, Leo Rowe-Brown, Chris Dunsby, Chris Bakal
How cancer cells determine their shape in response to three-dimensional (3D) geometric and mechanical cues is unclear. We develop an approach to quantify the 3D cell shape of over 60,000 melanoma cells in collagen hydrogels using high-throughput stage-scanning oblique plane microscopy (ssOPM). We identify stereotypic and environmentally dependent changes in shape and protrusivity depending on whether a cell is proximal to a flat and rigid surface or is embedded in a soft environment. Environmental sensitivity metrics calculated for small molecules and gene knockdowns identify interactions between the environment and cellular factors that are important for morphogenesis...
April 16, 2024: Cell Reports