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collagen MMP valve heart immunohistochemistry

A Arcucci, M R Ruocco, F Albano, G Granato, V Romano, G Corso, C Bancone, E De Vendittis, A Della Corte, S Montagnani
Ascending aortic aneurysm (AsAA) is a consequence of medial degeneration (MD), deriving from apoptotic loss of smooth muscle cells (SMC) and fragmentation of elastin and collagen fibers. Alterations of extracellular matrix structure and protein composition, typical of medial degeneration, can modulate intracellular pathways. In this study we examined the relevance of superoxide dismutase (SOD3) and Akt in AsAA pathogenesis, evaluating their tissue distribution and protein levels in ascending aortic tissues from controls (n=6), patients affected by AsAA associated to tricuspid aortic valve (TAV, n=9) or bicuspid aortic valve (BAV, n=9)...
2014: European Journal of Histochemistry: EJH
Su Leyman Karakoyun, Ozan Mustafa Gu Rsoy, Macit Kalçık, Sermin Coban Kökten, Mehmet Ozkan
Pannus formation is an infrequent but serious complication of prosthetic heart valve surgery. The cause of pannus is recognized as a bioreaction to the prostheses; histological investigations have shown that pannus comprises collagen and elastic tissues containing endothelial cells, chronic inflammatory cells, and myofibroblasts. However, the detailed mechanism of its formation has not been fully demonstrated. We aimed to evaluate the potential role of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-2 (MMP-2) in the pathogenesis of pannus formation in three patients with mechanical prosthetic heart valves...
January 2014: Türk Kardiyoloji Derneği Arşivi: Türk Kardiyoloji Derneğinin Yayın Organıdır
Ida Perrotta, Emilio Russo, Caterina Camastra, Gemma Filice, Giulio Di Mizio, Federica Colosimo, Pietrantonio Ricci, Sandro Tripepi, Andrea Amorosi, Franco Triumbari, Giuseppe Donato
AIMS: Calcific aortic stenosis is a progressive disease characterized by massive fibrosis andmineralization of the valve leaflets. The aim of this study was to determine whether the onset of native calcific aortic stenosis is associated primarily with matrix remodelling events, and particularly with elastin degradation. METHODS AND RESULTS: The immunohistochemical expression profile of matrix degradating enzymes and tenascin-C was investigated in both healthy and native calcified aortic valves...
September 2011: Histopathology
Ge Tao, Agata K Levay, Thomas Gridley, Joy Lincoln
Cardiac valves originate from endocardial cushions (EC) formed by endothelial-to-mesenchymal transformation (EMT) during embryogenesis. The zinc-finger transcription factor Snai1 has previously been reported to be important for EMT during organogenesis, yet its role in early valve development has not been directly examined. In this study we show that Snai1 is highly expressed in endothelial, and newly transformed mesenchyme cells during EC development. Mice with targeted snai1 knockdown display hypocellular ECs at E10...
November 15, 2011: Developmental Biology
R Shetty, P Pibarot, A Audet, R Janvier, F Dagenais, J Perron, C Couture, P Voisine, J P Després, P Mathieu
BACKGROUND: The durability of bioprosthetic valves is limited by structural valve degeneration (SVD) leading to bioprostheses (BPs) stenosis or regurgitation. We hypothesized that a lipid-mediated inflammatory mechanism is involved in the SVD of BPs. MATERIAL AND METHODS: Eighteen Freestyle stentless BP valves were explanted for SVD at a mean time of 5.9 +/- 3 years after implantation and were analysed by immunohistochemistry and transmission electron microscopy (TEM)...
June 2009: European Journal of Clinical Investigation
Rajeev Mahimkar, Anita Nguyen, Michael Mann, Che-Chung Yeh, Bo-Qing Zhu, Joel S Karliner, David H Lovett
INTRODUCTION: Myxomatous mitral valve "degeneration" with prolapse (MVP) is the most frequent form of nonischemic mitral valve disease. In myxomatous valves, interstitial cells express extracellular matrix-degrading enzymes and it has been postulated that matrix metalloproteinases (MMPs) contribute to these changes. METHODS: We generated mice with cardiac-specific expression of constitutively active MMP-2 under the control of the alpha-myosin heavy chain promoter...
September 2009: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
Elizabeth H Stephens, K Jane Grande-Allen
BACKGROUND AND AIM OF THE STUDY: Although the six-month-old pig is commonly used as a model to study human heart valve biology and various age-specific valve diseases, the correlation of porcine valve biology and development with that of humans has not been thoroughly assessed. Given the important role of the matrix in valve function, the aim of this study was to characterize porcine valve matrix structure and collagen turnover during development and aging. METHODS: Porcine aortic valves (AV) and mitral valves (MV) were examined throughout fetal development and postnatally at six weeks, six months and six years, using Movat pentachrome staining and immunohistochemistry for collagen III, markers of collagen synthesis (molecular chaperone HSP47, hydroxylating enzyme prolyl-4-hydroxylase (P4H), cross-linking enzyme lysyl oxidase (LOX)) and collagen degradation (matrix metalloproteinase (MMP)-13), and a marker of an 'activated' cellular phenotype, smooth muscle alpha-actin (SMalphaA)...
November 2007: Journal of Heart Valve Disease
E Herpel, M Pritsch, A Koch, T J Dengler, P Schirmacher, Ph A Schnabel
AIMS: To investigate whether or not there are differences in the distribution of extracellular matrix (ECM) proteins and matrix metalloproteinases (MMPs) in end-stage heart failure underlying different cardiomyopathies. METHODS AND RESULTS: Thirty-nine explanted human hearts were investigated: 15 with dilated cardiomyopathy (DCM), 17 with ischaemic cardiomyopathy (ICM) and seven with valvular cardiomyopathy (VCM). Transmural samples from four different sites were investigated...
May 2006: Histopathology
George J Koullias, Dimitris P Korkolis, Pars Ravichandran, Amanda Psyrri, Ioannis Hatzaras, John A Elefteriades
OBJECTIVE: The degeneration of bicuspid aortic valve and its frequent association with ascending aortic pathology, point to a still unidentified genetic tissue defect with unknown mediators. Metalloproteinases (MMPs) are lytic enzymes that have been strongly implicated in aneurysm formation. The purpose of this study was to detect the presence of these enzymes in aortic valvular tissue in healthy and diseased aortic valves with or without the presence of synchronous ascending aortic pathology...
December 2004: European Journal of Cardio-thoracic Surgery
Koichi Tamura, Yuichi Sugisaki, Shunichi Ogawa, Hitoshi Yamauchi, Ryozo Okada
A case with tumorous deformity of the posterior mitral valve leaflet after spontaneous chordal rupture in a child is described. A partial rupture in the chordae tendineae of the posterior mitral leaflet was found by echocardiography in a 9-year-old Japanese boy. Tumorous bulging was gradually developed in the leaflet and was surgically excised 5 years later. Multiple nodular tumors were found on the atrial surface of the posterior mitral leaflet. Histological examination revealed that the tumorous bulging consisted of myxomatous materials in which collagen fibrils and very fine elastic fibers were distributed loosely and irregularly...
January 2003: Pathology International
Elena Rabkin, Simon P Hoerstrup, Masanori Aikawa, John E Mayer, Frederick J Schoen
BACKGROUND AND AIM OF THE STUDY: Contemporary tissue valves are non-viable, and unable to grow, repair or remodel. It was postulated that tissue-engineered heart valves (TEHV) fabricated from autologous cells and a biodegradable scaffold could yield a dynamic progression of cell phenotype and extracellular matrix (ECM), in vitro and in vivo, and ultimately recapitulate native valve microscopic architecture. METHODS: Trileaflet valve constructs were fabricated from poly-4-hydroxybutyrate-coated polyglycolic acid seeded with ovine endothelial and carotid artery medial cells, cultured in vitro for 4-14 days in a pulse duplicator, implanted as pulmonary valves in five lambs, and explanted at 4-20 weeks...
May 2002: Journal of Heart Valve Disease
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