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immunohistochemistry heart valve

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https://www.readbyqxmd.com/read/27610947/characterization-of-immunogenic-neu5gc-in-bioprosthetic-heart-valves
#1
Eliran Moshe Reuven, Shani Leviatan Ben-Arye, Tal Marshanski, Michael E Breimer, Hai Yu, Imen Fellah-Hebia, Jean-Christian Roussel, Cristina Costa, Manuel Galiñanes, Rafael Mañez, Thierry Le Tourneau, Jean-Paul Soulillou, Emanuele Cozzi, Xi Chen, Vered Padler-Karavani
BACKGROUND: The two common sialic acids (Sias) in mammals are N-acetylneuraminic acid (Neu5Ac) and its hydroxylated form N-glycolylneuraminic acid (Neu5Gc). Unlike most mammals, humans cannot synthesize Neu5Gc that is considered foreign and recognized by circulating antibodies. Thus, Neu5Gc is a potential xenogenic carbohydrate antigen in bioprosthetic heart valves (BHV) that tend to deteriorate in time within human patients. METHODS: We investigated Neu5Gc expression in non-engineered animal-derived cardiac tissues and in clinically used commercial BHV, and evaluated Neu5Gc immunogenicity on BHV through recognition by human anti-Neu5Gc IgG...
September 2016: Xenotransplantation
https://www.readbyqxmd.com/read/27511979/occult-transthyretin-cardiac-amyloid-in-severe-calcific-aortic-stenosis-prevalence-and-prognosis-in-patients-undergoing-surgical-aortic-valve-replacement
#2
Thomas A Treibel, Marianna Fontana, Janet A Gilbertson, Silvia Castelletti, Steven K White, Paul R Scully, Neil Roberts, David F Hutt, Dorota M Rowczenio, Carol J Whelan, Michael A Ashworth, Julian D Gillmore, Philip N Hawkins, James C Moon
BACKGROUND: Calcific aortic stenosis (cAS) affects 3% of individuals aged >75 years, leading to heart failure and death unless the valve is replaced. Wild-type transthyretin cardiac amyloid is also a disorder of ageing individuals. Prevalence and clinical significance of dual pathology are unknown. This study explored the prevalence of wild-type transthyretin amyloid in cAS by myocardial biopsy, its imaging phenotype and prognostic significance. METHODS AND RESULTS: A total of 146 patients with severe AS requiring surgical valve replacement underwent cardiovascular magnetic resonance and intraoperative biopsies; 112 had cAS (75±6 years; 57% men)...
August 2016: Circulation. Cardiovascular Imaging
https://www.readbyqxmd.com/read/27353274/histopathological-assessment-of-calcification-and-inflammation-of-calcific-aortic-valves-from-patients-with-and-without-diabetes-mellitus
#3
Josephin Mosch, Christian A Gleissner, Simon Body, Elena Aikawa
BACKGROUND: Calcific aortic valve disease (CAVD) is the most common valvular heart disease and likely evolves from inflammatory pre-conditions in the valve. Type II diabetes mellitus (DMII) has been associated with pathogenesis of CAVD, however, the mechanism initiating CAVD in DMII is not well understood and the human valve pathology in DMII has not been described. We therefore performed quantitative histological analyses of aortic valves of CAVD patients with and without DMII. METHODS: CAVD human aortic valves (n=45) obtained after surgical valve replacement were examined macroscopically with gross measurements of calcified areas...
June 29, 2016: Histology and Histopathology
https://www.readbyqxmd.com/read/27344132/lymphangiogenesis-is-increased-in-heart-valve-endocarditis
#4
Eetu Niinimäki, Ari A Mennander, Timo Paavonen, Ivana Kholová
BACKGROUND: Inflammation-associated lymphangiogenesis (IAL) has been identified as part of several acute and chronic inflammation. Sparse data exist on lymphatics during endocarditis. MATERIALS AND METHODS: Fifty-two patients with surgically resected valves were included. Endocarditis was present in 18 aortic and 10 mitral valves. Controls consisted of 15 degenerative aortic and 9 degenerative mitral valves. There were 22 males with endocarditis and 17 males in controls...
September 15, 2016: International Journal of Cardiology
https://www.readbyqxmd.com/read/27141955/associations-of-mmp-2-bax-and-bcl-2-mrna-and-protein-expressions-with-development-of-atrial-fibrillation
#5
Shu-Ling Diao, Hui-Pu Xu, Bei Zhang, Bao-Xin Ma, Xian-Liang Liu
BACKGROUND To examine changes of mRNA and protein expressions of MMP-2, Bcl-2, and BAX in atrial fibrillation (AF) patients, and investigate the correlations among these 3 biomarkers. MATERIAL AND METHODS Rheumatic heart disease patients (n=158) undergoing cardiac surgical procedures for mitral valve repair or replacement were included as the AF group (n=123), containing paroxysmal AF (n=42), persistent AF (n=36), and permanent AF (n=45). Rheumatic heart disease patients with sinus rhythm (SR) (n=35) were enrolled as the SR group (control group)...
2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/27117366/-preliminary-study-of-the-expression-of-connective-tissue-growth-factor-in-papillary-muscles-of-the-patients-with-rheumatic-heart-disease
#6
Y N Wang, T Li, J R Gu, B Y Yu
OBJECTIVE: To investigate the expression and the effect of connective tissue growth factor (CTGF) on rheumatic myocardial fibrosis of rheumatic heart disease (RHD). METHODS: The papillary muscles samples were obtained from patients with RHD during mitral valve replacement.The expression of TGF-β1, CTGF mRNA and CTGF protein were detected with semiquantitative RT-PCR technique and immunohistochemistry technologyin the papillary muscles cell from 41RHD patients and 20 normal papillary muscles samples...
April 19, 2016: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/26897823/the-role-of-interleukin-13-in-patients-with-rheumatic-valvular-fibrosis-a-clinical-and-histological-study
#7
Qi Liu, Wei-Hua Qiao, Fei-fei Li, Peg Deng, Zhi-Wei Hu
BACKGROUND AND AIM OF THE STUDY: Interleukin (IL)-13 is a major inducer of fibrosis in many chronic infectious diseases, yet few studies have reported its role in valvular fibrosis in patients with rheumatic heart disease (RHD). The study aim was to investigate the role of IL-13 in mitral valvular fibrosis in patients with RHD. METHODS: Peripheral blood samples were collected from surgical patients with RHD (n = 18) and from healthy controls (n = 9). Serum levels of IL-13 and interferon (IFN)-gamma were analyzed using ELISA...
July 2015: Journal of Heart Valve Disease
https://www.readbyqxmd.com/read/26897815/c4b-binding-protein-deposition-is-induced-in-diseased-aortic-heart-valves-coinciding-with-c3d
#8
Maria Jose Dikhoff, Mimi ter Weeme, Alexander B A Vonk, Koba Kupreishvili, Anna M Blom, Paul A J Krijnen, Wim Stooker, Hans W M Niessen
BACKGROUND AND AIM OF THE STUDY: It has been found recently that activated complement is more widespread in diseased aortic valves compared to the endogenous complement inhibitors C1-inhibitor and clusterin. Previously, another endogenous inhibitor of complement, C4b-binding protein (C4BP) has been described in atherosclerotic diseased coronary arteries. The study aim was to analyze C4BP levels in diseased aortic valves. METHODS: Aortic valve tissue was derived from surgical procedures and classified as 'degenerative', 'atherosclerotic' or 'atherosclerotic with bacterial infection'...
July 2015: Journal of Heart Valve Disease
https://www.readbyqxmd.com/read/26885055/growth-differentiation-factor-15-gdf-15-novel-biomarker-for-assessing-atrial-fibrosis-in-patients-with-atrial-fibrillation-and-rheumatic-heart-disease
#9
Yong-Ming Zhou, Ming-Jiang Li, Yan-Li Zhou, Le-Le Ma, Xin Yi
OBJECTIVE: Growth differentiation factor-15 (GDF-15) has been identified as a strong biomarker of cardiovascular diseases; however, no evidence are available concerning the relationship of GDF-15 and atrial fibrosis in patients with atrial fibrillation (AF) and rheumatic heart disease (RHD). METHODS: Twenty patients with rheumatic heart disease were divided into two groups, 10 cases with AF and 10 cases with sinus rhythm (SR). Clinical data and blood samples were collected; left atrial appendage was taken by the surgeon in the process of valve replacement...
2015: International Journal of Clinical and Experimental Medicine
https://www.readbyqxmd.com/read/26823820/fibroblast-growth-factor-21-is-positively-associated-with-atrial-fibrosis-in-atrial-fibrillation-patients-with-rheumatic-heart-disease
#10
Rui Wang, Xin Yi, Xiaoyan Li, Xuejun Jiang
OBJECTIVE: Fibroblast growth factor-21 (FGF-21) has been discovered as a strong hormone, plays an important role in lipid metabolism, glucose metabolism, associated with several diseases such as obesity, metabolic syndrome, diabetes mellitus, and cardiovascular events; however, no evidence is available concerning the relationship of FGF-21 and atrial fibrosis in patients with atrial fibrillation (AF) and rheumatic heart disease (RHD). METHODS: Twenty-four rheumatic heart disease patients were divided into two groups, 12 cases with AF and 12 cases with sinus rhythm (SR)...
2015: International Journal of Clinical and Experimental Pathology
https://www.readbyqxmd.com/read/26823728/proteomic-analysis-of-mitral-valve-in-lewis-rat-with-acute-rheumatic-heart-disease
#11
Wenting Li, Zhiyu Zeng, Chun Gui, Huilei Zheng, Weiqiang Huang, Heng Wei, Danping Gong
Rheumatic heart disease (RHD) makes a heavy burden in human lives and economy. The proteomic analysis of acute rheumatic heart disease (ARHD) can provide precious data to study RHD at the early stages, but no one has looked into. So based on our early research we applied the method of continuous GAS stimulation on Lewis rats to duplicate the animal model of ARHD. And the mitral valves of rats in control group (n=10) and ARHD group (n=10) were selected for proteomic analysis of ARHD with the iTRAQ labeling based 2D LC-ESI-MS/MS quantitative technology...
2015: International Journal of Clinical and Experimental Pathology
https://www.readbyqxmd.com/read/26822476/common-arterial-trunk-and-ventricular-non-compaction-in-lrp2-knockout-mice-indicate-a-crucial-role-of-lrp2-in-cardiac-development
#12
Maria E Baardman, Mathijs V Zwier, Lambertus J Wisse, Adriana C Gittenberger-de Groot, Wilhelmina S Kerstjens-Frederikse, Robert M W Hofstra, Angelika Jurdzinski, Beerend P Hierck, Monique R M Jongbloed, Rolf M F Berger, Torsten Plösch, Marco C DeRuiter
Lipoprotein-related receptor protein 2 (LRP2) is important for development of the embryonic neural crest and brain in both mice and humans. Although a role in cardiovascular development can be expected, the hearts ofLrp2knockout (KO) mice have not yet been investigated. We studied the cardiovascular development ofLrp2KO mice between embryonic day 10.5 (E10.5) and E15.5, applying morphometry and immunohistochemistry, using antibodies against Tfap2α (neural crest cells), Nkx2.5 (second heart field), WT1 (epicardium derived cells), tropomyosin (myocardium) and LRP2...
April 2016: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/26472242/muscleblind-like-1-is-required-for-normal-heart-valve-development-in-vivo
#13
Ryan J Coram, Samantha J Stillwagon, Anuradha Guggilam, Michael W Jenkins, Maurice S Swanson, Andrea N Ladd
BACKGROUND: Development of the valves and septa of the heart depends on the formation and remodeling of the endocardial cushions in the atrioventricular canal and outflow tract. These cushions are populated by mesenchyme produced from the endocardium by epithelial-mesenchymal transition (EMT). The endocardial cushions are remodeled into the valves at post-EMT stages via differentiation of the mesenchyme and changes in the extracellular matrix (ECM). Transforming growth factor β (TGFβ) signaling has been implicated in both the induction of EMT in the endocardial cushions and the remodeling of the valves at post-EMT stages...
October 15, 2015: BMC Developmental Biology
https://www.readbyqxmd.com/read/26371175/pregnancy-induced-remodeling-of-heart-valves
#14
COMPARATIVE STUDY
Caitlin M Pierlot, Andrew D Moeller, J Michael Lee, Sarah M Wells
Recent studies have demonstrated remodeling of aortic and mitral valves leaflets under the volume loading and cardiac expansion of pregnancy. Those valves' leaflets enlarge with altered collagen fiber architecture, content, and cross-linking and biphasic changes (decreases, then increases) in extensibility during gestation. This study extends our analyses to right-sided valves, with additional compositional measurements for all valves. Valve leaflets were harvested from nonpregnant heifers and pregnant cows...
November 2015: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/26341225/characterization-of-cd133-antibody-directed-recellularized-heart-valves
#15
J Koudy Williams, Elizabeth S Miller, Magan R Lane, Anthony Atala, James J Yoo, James E Jordan
CD133mAb conjugation (CD133-C) hastens in vivo recellularization of decellularized porcine heart valve scaffolds when placed in the pulmonary position of sheep. We now characterize this early cellularization process 4 h, 3, 7, 14, 30, or 90 days post-implantation. Quantitative immunohistochemistry identified cell types as well as changes in cell markers and developmental cues. CD133(+)/CD31(-) cells adhered to the leaflet surface of CD133-C leaflets by 3 days and transitioned to native leaflet-like CD133(-)/CD31(+) cells by 30 days...
October 2015: Journal of Cardiovascular Translational Research
https://www.readbyqxmd.com/read/26318798/changes-in-the-expression-of-th17-cell-associated-cytokines-in-the-development-of-rheumatic-heart-disease
#16
Yun Wen, Zhiyu Zeng, Chun Gui, Lang Li, Wenting Li
BACKGROUND: Autoimmunity plays a critical role in the development of rheumatic heart disease (RHD). Recent studies have linked Th17 cells to the autoimmune mechanism associated with RHD. This study aimed to investigate changes in Th17 cell-related cytokine expression in acute and chronic RHD. METHODS: We established a Lewis rat model of experimental RHD, which was induced by inactivated Group A streptococci and complete Freund's adjuvant. After 7- and 24-week intervention treatments, we measured serum levels of interleukin-17 (IL-17) and IL-6, key cytokines associated with Th17 cells, using a Luminex liquichip method, and levels of IL-17 and IL-6 in heart tissues using immunohistochemical assays...
November 2015: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
https://www.readbyqxmd.com/read/26268418/histology-and-immunohistochemistry-of-the-cardiac-ventricular-structure-in-the-green-turtle-chelonia-mydas
#17
J K F S Braz, M L Freitas, M S Magalhães, M F Oliveira, M S M O Costa, N S Resende, N K Clebis, N B Silva, C E B Moura
This study describes the implications of cardiac ventricular microscopy in Chelonia mydas relating to its ability to dive. For this work, 11 specimens of the marine turtle species C. mydas found dead on the coast of Rio Grande do Norte (Northeast Brazil) were used. After necropsy, fragments of the cardiac ventricular wall were fixed in 10% buffered formaldehyde solution for 24 h and then subjected to routine processing for light and scanning electron microscopy (SEM). The ventricle in this species is formed by the epicardium, myocardium and endocardium...
August 2016: Anatomia, Histologia, Embryologia
https://www.readbyqxmd.com/read/26186298/heart-fat-infiltration-in-subjects-with-and-without-coronary-artery-disease
#18
Gloria Mazzali, Francesco Fantin, Elena Zoico, Anna Sepe, Clara Bambace, Silvia Faccioli, Martina Pedrotti, Francesca Corzato, Vanni Rizzatti, Giuseppe Faggian, Rocco Micciolo, Saverio Cinti, Francesco Santini, Mauro Zamboni
CONTEXT: Fat may accumulate around the heart in epicardial adipose tissue or inside the heart as lipid droplets (LDs). OBJECTIVE: To compare myocardial steatosis between subjects with and without coronary artery disease (CAD and non-CAD) and to identify which cells contain LDs. DESIGN: Body mass index, waist circumference, glucose, insulin, homeostasis model assessment index, leptin, adiponectin, and high-sensitivity C-reactive protein were evaluated in CAD and non-CAD subjects...
September 2015: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/26182623/cryoprotective-effect-and-optimal-concentration-of-trehalose-on-aortic-valve-homografts
#19
COMPARATIVE STUDY
Qing Chang, C Chen Cheng, Hui Jing, C Jian Sheng, T Yi Wang
BACKGROUND AND AIM OF THE STUDY: Cryopreservation allows for the long-term storage of aortic homografts, but a high risk of calcification and degeneration is often observed following their transplantation. The study aim was to investigate the cryoprotective effect of trehalose on aortic valve homografts preserved in liquid nitrogen, and to determine the optimal trehalose concentration for such purpose. METHODS: Aortic valve homografts obtained from New Zealand White rabbits were processed using different protectants...
January 2015: Journal of Heart Valve Disease
https://www.readbyqxmd.com/read/26138278/de-endothelialized-aortic-homografts-a-promising-scaffold-material-for-tissue-engineered-heart-valves
#20
Hui Jing, Zhenzhen Wang, Qing Chang
This study was designed to investigate the feasibility of de-endothelialized aortic homografts as a scaffold for tissue-engineered heart valves. Aortic homografts obtained from donor rabbits were treated either with collagenase to eliminate endotheliocytes or with the enzyme-detergent-nuclease method to remove all cell components. Then biomechanical properties of fresh, de-endothelialized and acellular homografts were investigated comparatively. The inflammation potential and immunogenicity were also assessed after allogenic transplantation...
2014: Cells, Tissues, Organs
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