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Clean room

Jennifer L Cadnum, Aaron A Shaikh, Christina T Piedrahita, Annette L Jencson, Emily L Larkin, Mahmoud A Ghannoum, Curtis J Donskey
Mobile ultraviolet-C (UV-C) light room decontamination devices are frequently used as an adjunct to standard cleaning in healthcare facilities, but their efficacy in killing Candida species is not clear. In laboratory testing, the emerging multidrug-resistant Candida auris and 2 other Candida species were significantly less susceptible to killing by UV-C than methicillin-resistant Staphylococcus aureus. Infect Control Hosp Epidemiol 2017;1-3.
November 21, 2017: Infection Control and Hospital Epidemiology
Samuel Yui, Shanom Ali, Monika Muzslay, Annette Jeanes, A Peter R Wilson
OBJECTIVE To identify, using a novel enhanced method of recovery, environmental sites where spores of Clostridium difficile persist despite cleaning and hydrogen peroxide aerial decontamination. DESIGN Cohort study. SETTING Tertiary referral center teaching hospital. METHODS In total, 16 sites representing high-frequency contact or difficult-to-clean surfaces in a single-isolation room or bed area in patient bed bays were sampled before and after terminal or hydrogen peroxide disinfection using a sponge swab...
November 16, 2017: Infection Control and Hospital Epidemiology
Farah Adilah Jamaludin, Mohd Zainal Abidin Ab-Kadir, Mahdi Izadi, Norhafiz Azis, Jasronita Jasni, Muhammad Syahmi Abd-Rahman
Located near the equator, Malaysia is a country with one of the highest lightning densities in the world. Lightning contributes to 70% of the power outages in Malaysia and affects power equipment, automated network systems, causes data losses and monetary losses in the nation. Therefore, consideration of insulator evaluation under lightning impulses can be crucial to evaluate and attempt to overcome this issue. This paper presents a new approach to increase the electrical performance of polymer insulators using a Room Temperature Vulcanisation (RTV) coating...
2017: PloS One
Karen M Miernyk, Carolynn K DeByle, Karen M Rudolph
BACKGROUND: Culture-independent molecular analyses allow researchers to identify diverse microorganisms. This approach requires microbiological DNA repositories. The standard for DNA storage is liquid nitrogen or ultralow freezers. These use large amounts of space, are costly to operate, and could fail. Room temperature DNA storage is a viable alternative. In this study, we investigated storage of bacterial DNA using two ambient storage matrices, Biomatrica DNAstable(®) Plus and GenTegra(®) DNA...
November 13, 2017: Biopreservation and Biobanking
Jonas Rehn, Kai Schuster
Analogously to the medical placebo effect, people seem to anticipate the quality of treatments based on external stimuli. In order to gain insights on the effect the built environment can have on a person's judgments and behavior with a particular focus on health related issues, a quantitative survey (N = 851) with four groups before and after the renovation of a rehabilitation clinic has been conducted. In line with an overall modernization of the clinic, the entrance, the lobby, and some patient rooms have been changed...
November 9, 2017: Behavioral Sciences
Shuoguo Li, Gang Ji, Yang Shi, Lasse Hyldgaard Klausen, Tongxin Niu, Shengliu Wang, Xiaojun Huang, Wei Ding, Xiang Zhang, Mingdong Dong, Wei Xu, Fei Sun
Cryo-correlative light and electron microscopy (cryo-CLEM) offers a unique way to analyze the high-resolution structural information of cryo-vitrified specimen by cryo-electron microscopy (cryo-EM) with the guide of the search for unique events by cryo-fluorescence microscopy (cryo-FM). To achieve cryo-FM, a trade-off must be made between the temperature and performance of objective lens. The temperature of specimen should be kept below devitrification while the distance between the objective lens and specimen should be short enough for high resolution imaging...
November 4, 2017: Journal of Structural Biology
Arianne P Oriá, Ana Claudia S Raposo, Nayone L L C Araújo, Felipe B Lima, Ali M Masmali
PURPOSE: To evaluate and compare three tear sampling methods using two grading scales for administering the tear ferning test (TFT) to healthy dogs. METHODS: In total, 90 dogs (180 eyes) were subjected to tear sampling using millimetered strips, reused after the Schirmer tear test (STT) (Schirmer group, SG). Then, the dogs were subdivided into three groups according to sampling approach: micropipette (MPG), microcapillary (MCG), and Schirmer sample 2 (S2G). The collected tears were dried on a clean microscope glass slide at room temperature and humidity...
November 7, 2017: Veterinary Ophthalmology
Xue Li, Imad A Haidar Ahmad, James Tam, Yan Wang, Gina Dao, Andrei Blasko
A Total Organic Carbon (TOC) based analytical method to quantitate trace residues of clean-in-place (CIP) detergents CIP100(®) and CIP200(®) on the surfaces of pharmaceutical manufacturing equipment was developed and validated. Five factors affecting the development and validation of the method were identified: diluent composition, diluent volume, extraction method, location for TOC sample preparation, and oxidant flow rate. Key experimental parameters were optimized to minimize contamination and to improve the sensitivity, recovery, and reliability of the method...
October 26, 2017: Journal of Pharmaceutical and Biomedical Analysis
Soran Jahangiri, Nicholas J Mosey
Nickel hydroxide is a material composed of two-dimensional layers that can be rolled up to form cylindrical nanotubes belonging to a class of inorganic metal hydroxide nanotubes that are candidates for applications in catalysis, energy storage, and microelectronics. The stabilities and other properties of this class of inorganic nanotubes have not yet been investigated in detail. The present study uses self-consistent-charge density-functional tight-binding calculations to examine the stabilities, mechanical properties, and electronic properties of nickel hydroxide nanotubes along with the energetics associated with the adsorption of water by these systems...
November 3, 2017: Nanotechnology
J-J Gallet, M G Silly, M El Kazzi, F Bournel, F Sirotti, F Rochet
Despite thermal silicon oxide desorption is a basic operation in semiconductor nanotechnology, its detailed chemical analysis has not been yet realized via time-resolved photoemission. Using an advanced acquisition system and synchrotron radiation, heating schedules with velocities as high as 100 K.s(-1) were implemented and highly resolved Si 2p spectra in the tens of millisecond range were obtained. Starting from a Si(111)-7 × 7 surface oxidized in O2 at room temperature (1.4 monolayer of oxygen), changes in the Si 2p spectral shape enabled a detailed chemical analysis of the oxygen redistribution at the surface and of the nucleation, growth and reconstruction of the clean silicon areas...
October 27, 2017: Scientific Reports
Jorge Vera, Jorge Ochoa, Monica Romero, Marino Vazquez-Carcaño, Cesar Omar Ramos-Gregorio, Ruben Rosas Aguilar, Alvaro Cruz, Philippe Sleiman, Ana Arias
INTRODUCTION: A prospective, multicentered, randomized clinical trial was designed to assess if controlled irrigation with cold saline could result in less incidence and intensity of postoperative pain in patients presenting with pulp necrosis and symptomatic apical periodontitis. METHODS: A total of 210 patients (presenting with necrotic uniradicular teeth with a diagnosis of symptomatic apical periodontitis and a preoperative visual analog scale (VAS) score higher than 7) were randomly allocated in the control or experimental group after the completion of shaping and cleaning procedures...
October 24, 2017: Journal of Endodontics
Kaisa Koskinen, Petra Rettberg, Rüdiger Pukall, Anna Auerbach, Lisa Wink, Simon Barczyk, Alexandra Perras, Alexander Mahnert, Diana Margheritis, Gerhard Kminek, Christine Moissl-Eichinger
BACKGROUND: The ExoMars 2016 mission, consisting of the Trace Gas Orbiter and the Schiaparelli lander, was launched on March 14 2016 from Baikonur, Kazakhstan and reached its destination in October 2016. The Schiaparelli lander was subject to strict requirements for microbial cleanliness according to the obligatory planetary protection policy. To reach the required cleanliness, the ExoMars 2016 flight hardware was assembled in a newly built, biocontrolled cleanroom complex at Thales Alenia Space in Turin, Italy...
October 25, 2017: Microbiome
Xuezhi Ma, Qiushi Liu, Da Xu, Yangzhi Zhu, Sanggon Kim, Yongtao Cui, Lanlan Zhong, Ming Liu
A simple and clean method of transferring two-dimensional (2D) materials plays a critical role in the fabrication of 2D electronics, particularly the heterostructure devices based on the artificial vertical stacking of various 2D crystals. Currently, clean transfer techniques rely on sacrificial layers or bulky crystal flakes (e.g., hexagonal boron nitride) to pick up the 2D materials. Here, we develop a capillary-force-assisted clean-stamp technique that uses a thin layer of evaporative liquid (e.g., water) as an instant glue to increase the adhesion energy between 2D crystals and polydimethylsiloxane (PDMS) for the pick-up step...
October 27, 2017: Nano Letters
A Inuwa, A Lunt, C Czuprynski, G Miller, S A Rankin
Although frozen dairy desserts have a strong record of safety, recent outbreaks of foodborne disease linked to ice creams have brought new attention to this industry. There is concern that small-scale frozen dessert equipment may not comply with or be reviewed against published comprehensive design and construction sanitation specifications (National Sanitation Foundation or 3-A sanitary standards). Equipment sanitary design issues may result in reduced efficacy of cleaning and sanitation, thus increasing the likelihood of postprocess contamination with pathogenic bacteria...
October 19, 2017: Journal of Food Protection
Flavien Blary, Julien Chabé, Aziz Ziad, Julien Borgnino, Yan Fanteï-Caujolle, Arnaud Liotard, Frédéric Falzon
Optical system performances can be affected by local optical turbulence created by its surrounding environment (telescope dome, clean room, or atmospheric layer). This paper follows a previous one introducing the INdoor TurbulENce SEnsor (INTENSE) instrument for optical turbulence characterization in a local area by exploitation of laser beam angle-of-arrival fluctuations. After a brief summary of the theoretical background, we present in this part results obtained using the INTENSE instrument in various optical integration testing clean rooms and telescope domes, each with specific air behavior conditions...
August 1, 2017: Applied Optics
Chang Liu, Ya-Huei Chang, Jianan Chen, Shien-Ping Feng
High-index-faceted nano-/microcrystals exhibit enhanced catalytic activity and can thus serve as new-generation catalysts owing to their high density of low-coordinated atoms, leading to significantly enhanced catalytic activity. In this study, an effective electrochemical approach termed cyclic scanning electrodeposition (CSE) was developed to convert a thin Cu film into Cu2O concave octahedrons enclosed by 24 {344} high-index facets at room temperature with high yield and high throughput. The formation mechanism and the role of each ion in the electrolyte were systematically studied, which facilitated the design of a high-index-faceted metal/metal oxide through CSE...
November 8, 2017: ACS Applied Materials & Interfaces
Jemere Bekele Harito, Andrew T Campbell, Kristoffer R Tysnes, Lucy J Robertson
Proof-of-principle of lectin-magnetic separation (LMS) for isolating Toxoplasma oocysts (pre-treated with 0.5% acidified pepsin (AP)) from water for subsequent detection by microscopy or molecular methods has been shown. However, application of this technique in the routine water-analysis laboratory requires that the method is tested, modified, and optimized. The current study describes attempts to apply the LMS technique on supernatants from water samples previously analyzed for contamination with Cryptosporidium and Giardia using standard methods, and the supernatant following immunomagnetic separation (IMS) retained...
October 7, 2017: Water Research
Lauren P Knelson, Gemila K Ramadanovic, Luke F Chen, Rebekah W Moehring, Sarah S Lewis, William A Rutala, David J Weber, Daniel J Sexton, Deverick J Anderson
No abstract text is available yet for this article.
October 13, 2017: Infection Control and Hospital Epidemiology
Lynn El Haddad, Shashank S Ghantoji, Mark Stibich, Jason B Fleming, Cindy Segal, Kathy M Ware, Roy F Chemaly
BACKGROUND: Environmental cleanliness is one of the contributing factors for surgical site infections in the operating rooms (ORs). To decrease environmental contamination, pulsed xenon ultraviolet (PX-UV), an easy and safe no-touch disinfection system, is employed in several hospital environments. The positive effect of this technology on environmental decontamination has been observed in patient rooms and ORs during the end-of-day cleaning but so far, no study explored its feasibility between surgical cases in the OR...
October 10, 2017: BMC Infectious Diseases
Richard Bruch, André Kling, Gerald A Urban, Can Dincer
In recent years, biomarker diagnostics became an indispensable tool for the diagnosis of human disease, especially for the point-of-care diagnostics. An easy-to-use and low-cost sensor platform is highly desired to measure various types of analytes (e.g., biomarkers, hormones, and drugs) quantitatively and specifically. For this reason, dry film photoresist technology - enabling cheap, facile, and high-throughput fabrication - was used to manufacture the microfluidic biosensor presented here. Depending on the bioassay used afterwards, the versatile platform is capable of detecting various types of biomolecules...
September 19, 2017: Journal of Visualized Experiments: JoVE
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