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lucilia sericata

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https://www.readbyqxmd.com/read/28621210/effects-of-lucilia-sericata-on-wound-healing-in-streptozotocin-induced-diabetic-rats-and-analysis-of-its-secretome-at-the-proteome-level
#1
F K Tombulturk, M Kasap, M Tuncdemir, E Polat, S Sirekbasan, A Kanli, G Kanigur-Sultuybek
The use of Lucilia sericata larvae on the healing of wounds in diabetics has been reported. However, the role of the excretion/secretion (ES) products of the larvae in treatment of diabetic wounds remains unknown. This study investigated whether application of the ES products of L. sericata on the wound surface could improve the impaired wound healing in streptozotocin-induced diabetic rats. Additional analysis was performed to understand proteome content of L. sericata secretome to understand ES contribution at the molecular level...
January 1, 2017: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/28586212/species-identification-of-necrophagous-insect-eggs-based-on-amino-acid-profile-differences-revealed-by-direct-analysis-in-real-time-high-resolution-mass-spectrometry
#2
Justine E Giffen, Jennifer Y Rosati, Cameron M Longo, Rabi Ann Musah
The colonization of decomposing remains by necrophagous insects such as blow flies is of forensic importance because the progression through the various stages of insect development can be correlated to time of death. The ability to infer this information hinges on accurate determination of the fly species that are associated with the entomological evidence collected. This evidence can include eggs, larvae, pupae and puparial casings. Determination of the egg's identity is particularly challenging because the eggs of multiple species are morphologically very similar...
June 6, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28567525/forensic-entomotoxicology-revisited-towards-professional-standardisation-of-study-designs
#3
REVIEW
Erica I T da Silva, Brendan Wilhelmi, Martin H Villet
Forensic entomotoxicology is the use of insects as evidence of whether a toxicant is present in an environment such as a corpse, river or landscape. The earliest overtly forensic study was published in 1977, and since then, at least 63 papers have been published, most of them focused on the detection of toxicants in insects or on effects of toxicants on diverse insect indicator taxa. A comprehensive review of the published literature revealed various inconsistencies between studies that could be addressed by introducing standard protocols for such studies...
May 31, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28512294/the-potential-use-of-cuticular-hydrocarbons-and-multivariate-analysis-to-age-empty-puparial-cases-of-calliphora-vicina-and-lucilia-sericata
#4
Hannah E Moore, Jennifer L Pechal, M Eric Benbow, Falko P Drijfhout
Cuticular hydrocarbons (CHC) have been successfully used in the field of forensic entomology for identifying and ageing forensically important blowfly species, primarily in the larval stages. However in older scenes where all other entomological evidence is no longer present, Calliphoridae puparial cases can often be all that remains and therefore being able to establish the age could give an indication of the PMI. This paper examined the CHCs present in the lipid wax layer of insects, to determine the age of the cases over a period of nine months...
May 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28474172/age-estimation-during-the-blow-fly-intra-puparial-period-a-qualitative-and-quantitative-approach-using-micro-computed-tomography
#5
Daniel Martín-Vega, Thomas J Simonsen, Martina Wicklein, Martin J R Hall
Minimum post-mortem interval (minPMI) estimates often rely on the use of developmental data from blow flies (Diptera: Calliphoridae), which are generally the first colonisers of cadavers and, therefore, exemplar forensic indicators. Developmental data of the intra-puparial period are of particular importance, as it can account for more than half of the developmental duration of the blow fly life cycle. During this period, the insect undergoes metamorphosis inside the opaque, barrel-shaped puparium, formed by the hardening and darkening of the third instar larval cuticle, which shows virtually no external changes until adult emergence...
May 4, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28472319/evaluation-of-sterilized-artificial-diets-for-mass-rearing-the-lucilia-sericata-diptera-calliphoridae
#6
Le Zheng, Tawni L Crippen, Alan Dabney, Alex Gordy, Jeffery K Tomberlin
The impact of six sterilized diets (blood-yeast agar diet, decomposed beef liver diet, powdered beef liver diet, powdered fish diet, milk-based diet, and a chemically defined diet) on Lucilia sericata(Meigen) larvae reared at three densities (10 larvae, 20 larvae, and 40 larvae on 20 g diet) was determined in comparison to fresh beef liver as a control. Specifically, the effects of these diets on the following traits of L. sericatawere measured: 1) pupal weight, 2) pupation percentage, 3) eclosion percentage, as well as 4) adult longevity...
May 2, 2017: Journal of Medical Entomology
https://www.readbyqxmd.com/read/28454623/effects-of-different-storage-and-measuring-methods-on-larval-length-values-for-the-blow-flies-diptera-calliphoridae-lucilia-sericata-and-calliphora-vicina
#7
Valentina Bugelli, Carlo Pietro Campobasso, Marcel A Verhoff, Jens Amendt
In forensic entomology, the methods of sampling, killing, and storing entomological samples can affect larval age estimation, and, hence, the estimation of the minimum post-mortem interval. In the existing manuals, there is a certain amount of heterogeneity regarding methods and the recommendations for best practice in forensic entomology are insufficiently validated. This study evaluated three different length-measurement methods for larval stages and examined the influence of different killing and storing methods on the larval length of two forensically important blow flies, Lucilia sericata and Calliphora vicina...
May 2017: Science & Justice: Journal of the Forensic Science Society
https://www.readbyqxmd.com/read/28450516/genome-sequence-of-a-providencia-stuartii-strain-isolated-from-luciliasericata-salivary-glands
#8
Ye Yuan, Yu Zhang, Shuhua Fu, Tawni L Crippen, David K Visi, M Eric Benbow, Michael S Allen, Jeffery K Tomberlin, Sing-Hoi Sze, Aaron M Tarone
We present here the draft genome sequence of a Providencia stuartii strain, derived from the salivary glands of larval Lucilia sericata, a common blow fly important to forensic, medical, and veterinary science. The genome sequence will help dissect coinfections involving P. stuartii and Proteus mirabilis, as well as blow fly-bacteria interactions.
April 27, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28419727/endotoxin-testing-of-a-wound-debridement-device-containing-medicinal-lucilia-sericata-larvae
#9
Samantha Pickles, David Pritchard
Alimentary products of medicinal Lucilia sericata larvae are studied to determine their mechanisms of action, particularly in the contexts of wound debridement and disinfection. Furthermore, the larvae can be applied to patients in contained medical devices (such as the BioBag; BioMonde). Here, we tested the materials and larval content of the most commonly used debridement device (the "BB-50") to explore the possibility that endotoxins may be contributing to the bio-activity of the product, given that endotoxins are potent stimulants of cellular activation...
April 17, 2017: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/28409386/nocturnal-oviposition-behavior-of-blowflies-diptera-calliphoridae-in-the-southern-hemisphere-south-africa-and-australia-and-its-forensic-implications
#10
Kirstin A Williams, James F Wallman, Bryan D Lessard, Christopher R J Kavazos, D Nkosinathi Mazungula, Martin H Villet
Published research has offered contradictory evidence of the occurrence of nocturnal oviposition by carrion-breeding blowflies, a behavior that can affect the interpretation of forensic estimates of a minimum post mortem interval (minPMI) by up to 12 hours, depending on latitude and season. The majority of published studies are from the northern hemisphere. Field experiments were conducted in South Africa and Australia that extend observations to species of the southern hemisphere. Various vertebrate carrion was exposed at night in summer under different lunar phases and/or artificial lighting, and in woodland and pasture areas...
April 13, 2017: Forensic Science, Medicine, and Pathology
https://www.readbyqxmd.com/read/28402593/a-new-method-for-the-production-of-sterile-colonies-of-lucilia-sericata
#11
N E Gasz, M L Harvey
Maggot debridement therapy (MDT) refers to the use of blowfly larvae to clean or debride an infected wound. Most commonly, larvae of Lucilia sericata (Meigen) (Diptera: Calliphoridae) are used, and are sterilized prior to use to ensure no further bacterial infections are introduced during treatment. Current methods sterilize eggs from laboratory-reared blowfly colonies, after which sterile early second instar maggots can be provided to hospitals for use in treatment. Maggots not required for treatment are used for colony regeneration, in which sterility is not maintained...
April 12, 2017: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/28398264/factors-affecting-species-identifications-of-blow-fly-pupae-based-upon-chemical-profiles-and-multivariate-statistics
#12
William Kranz, Clinton Carroll, Darren A Dixon, John V Goodpaster, Christine J Picard
Alternative methods for the identification of species of blow fly pupae have been developed over the years that consist of the analyses of chemical profiles. However, the effect of biotic and abiotic factors that could influence the predictive manner for the tests have not been evaluated. The lipids of blowfly pupae (Cochliomyia macellaria, Lucilia cuprina, Lucilia sericata, and Phormia regina) were extracted in pentane, derivatized, and analyzed by total-vaporization solid phase microextraction gas chromatography-mass spectrometry (TV-SPME GC-MS)...
April 11, 2017: Insects
https://www.readbyqxmd.com/read/28374542/to-eat-or-get-heat-behavioral-trade-offs-between-thermoregulation-and-feeding-in-gregarious-necrophagous-larvae
#13
Jana Podhorna, Cindy Aubernon, Marie Borkovcova, Julien Boulay, Valery Hedouin, Damien Charabidze
The thermoregulation behavior of Lucilia sericata larvae (Diptera: Calliphoridae), a necrophagous species that feeds on vertebrate cadavers, was investigated. These larvae require high heat incomes to develop, and can elevate temperatures by forming large aggregates. We hypothesized that L. sericata larvae should continue to feed at temperatures up to 38°C, which can be reached inside larval masses. Thermal regulation behavior such as movement between a hot food spot and colder areas was also postulated. The hypotheses were tested by tracking for 1h the activity of single, starved third instar larvae in a Petri dish containing one food spot (FS) that was heated to a constant temperature of 25°C, 34°C or 38°C with an ambient temperature of 25°C...
April 4, 2017: Insect Science
https://www.readbyqxmd.com/read/28364721/effects-of-environmental-temperature-on-oviposition-behavior-in-three-blow-fly-species-of-forensic-importance
#14
Helen Ody, Mark T Bulling, Kate M Barnes
A number of factors are known to affect blow fly behavior with respect to oviposition. Current research indicates that temperature is the most significant factor. However temperature thresholds for oviposition in forensically important blow flies have not been well studied. Here, the oviposition behavior of three species of forensically important blow fly species (Calliphora vicina, Calliphora vomitoria and Lucilia sericata,) was studied under controlled laboratory conditions over a range of temperatures (10-40°C)...
March 14, 2017: Forensic Science International
https://www.readbyqxmd.com/read/28333104/spectral-signatures-of-immature-lucilia-sericata-meigen-diptera-calliphoridae
#15
Jodie-A Warren, T D Pulindu Ratnasekera, David A Campbell, Gail S Anderson
Hyperspectral remote sensing is an innovative technology with applications in many sciences and is a non-destructive method that may offer more precise aging within development stages. Hyperspectral reflectance measurements from the anterior, midsection, and posterior of Lucilia sericata (Meigen) larvae and pupae were conducted daily from samples of the developing insects beginning at second instar. Only midsection measurements were conducted on second instar larvae due to their size, to ensure that the measurement was not of reflective surroundings...
March 23, 2017: Insects
https://www.readbyqxmd.com/read/28235562/urate-oxidase-produced-by-lucilia-sericata-medical-maggots-is-localized-in-malpighian-tubes-and-facilitates-allantoin-production
#16
Andre Baumann, Marisa Skaljac, Rüdiger Lehmann, Andreas Vilcinskas, Zdenӗk Franta
Lucilia sericata maggots are the only species currently approved for maggot debridement therapy (MDT), an alternative treatment for chronic and recalcitrant wounds. Maggots promote wound debridement, disinfection and healing by producing a complex mixture of proteins, peptides and low-molecular-weight compounds in their secretions and excretions, but the individual components are not well characterized at the molecular level. Here we investigated the purine catabolism pathway in L. sericata, focusing on the production of allantoin by Urate Oxidase (UO), which is thought to promote wound healing...
February 22, 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28193269/how-flies-are-flirting-on-the-fly
#17
Courtney Eichorn, Michael Hrabar, Emma C Van Ryn, Bekka S Brodie, Adam J Blake, Gerhard Gries
BACKGROUND: Flies have some of the most elaborate visual systems in the Insecta, often featuring large, sexually dimorphic eyes with specialized "bright zones" that may have a functional role during mate-seeking behavior. The fast visual system of flies is considered to be an adaptation in support of their advanced flight abilities. Here, we show that the immense processing speed of the flies' photoreceptors plays a crucial role in mate recognition. RESULTS: Video-recording wing movements of abdomen-mounted common green bottle flies, Lucilia sericata, under direct light at 15,000 frames per second revealed that wing movements produce a single, reflected light flash per wing beat...
February 14, 2017: BMC Biology
https://www.readbyqxmd.com/read/28162132/wohlfahrtiimonas-chitiniclastica-current-insights-into-an-emerging-human-pathogen
#18
REVIEW
P Schröttner, W W Rudolph, U Damme, C Lotz, E Jacobs, F Gunzer
Since the first description of Wohlfahrtiimonas chitiniclastica in 2008, a number of well described case reports demonstrating its pathogenic role in humans have been published. Infections may be closely linked to flies, such as Wohlfahrtia magnifica, Lucilia sericata, Chrysomya megacephala or Musca domestica. These insects are potent vectors for the distribution of W. chitiniclastica causing local or systemic infections originating from wounds infested with fly larvae. However, other potential sources of transmission of W...
May 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28117913/quality-control-of-a-medicinal-larval-lucilia-sericata-debridement-device-based-on-released-gelatinase-activity
#19
S F Pickles, D I Pritchard
Lucilia sericata Meigen (Diptera: Calliphoridae) larvae are manufactured worldwide for the treatment of chronic wounds. Published research has confirmed that the primary clinical effect of the product, debridement (the degradation of non-viable wound tissue), is accomplished by a range of enzymes released by larvae during feeding. The quality assessment of larval activity is currently achieved during production using meat-based assays, which monitor insect growth and/or the reduction in substrate mass. To support this, the present authors developed a complementary radial diffusion enzymatic assay to produce a visual and measureable indication of the activity of larval alimentary products (LAP) collected under standardized conditions, against a gelatin substrate...
January 24, 2017: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/28019718/excretions-secretions-from-medicinal-larvae-lucilia-sericata-inhibit-complement-activation-by-two-mechanisms
#20
Tetsuro Tamura, Gwendolyn Cazander, Suzan H M Rooijakkers, Leendert A Trouw, Peter H Nibbering
Larvae of the blowfly Lucilia sericata facilitate wound healing by removing dead tissue and biofilms from non-healing and necrotic wounds. Another beneficial action of larvae and their excretions/secretions (ES) is down-regulation of excessive inflammation. As prolonged complement activation is key to excessive inflammation, the aim of this study was to elucidate the mechanisms underlying the anti-complement activities of ES. Results revealed that heat sensitive serine proteases in ES degrade multiple complement proteins in all steps of the three complement activation pathways...
December 26, 2016: Wound Repair and Regeneration
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