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https://www.readbyqxmd.com/read/28419727/endotoxin-testing-of-a-wound-debridement-device-containing-medicinal-lucilia-sericata-larvae
#1
Samantha Pickles, David Pritchard
Alimentary products of medicinal Lucilia sericata larvae are studied to determine their mechanisms of action, particularly in the contexts of wound debridement and disinfection. Furthermore, the larvae can be applied to patients in contained medical devices (such as the BioBag; BioMonde). Here, we tested the materials and larval content of the most commonly used debridement device (the "BB-50") to explore the possibility that endotoxins may be contributing to the bio-activity of the product, given that endotoxins are potent stimulants of cellular activation...
April 17, 2017: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/28409386/nocturnal-oviposition-behavior-of-blowflies-diptera-calliphoridae-in-the-southern-hemisphere-south-africa-and-australia-and-its-forensic-implications
#2
Kirstin A Williams, James F Wallman, Bryan D Lessard, Christopher R J Kavazos, D Nkosinathi Mazungula, Martin H Villet
Published research has offered contradictory evidence of the occurrence of nocturnal oviposition by carrion-breeding blowflies, a behavior that can affect the interpretation of forensic estimates of a minimum post mortem interval (minPMI) by up to 12 hours, depending on latitude and season. The majority of published studies are from the northern hemisphere. Field experiments were conducted in South Africa and Australia that extend observations to species of the southern hemisphere. Various vertebrate carrion was exposed at night in summer under different lunar phases and/or artificial lighting, and in woodland and pasture areas...
April 13, 2017: Forensic Science, Medicine, and Pathology
https://www.readbyqxmd.com/read/28402593/a-new-method-for-the-production-of-sterile-colonies-of-lucilia-sericata
#3
N E Gasz, M L Harvey
Maggot debridement therapy (MDT) refers to the use of blowfly larvae to clean or debride an infected wound. Most commonly, larvae of Lucilia sericata (Meigen) (Diptera: Calliphoridae) are used, and are sterilized prior to use to ensure no further bacterial infections are introduced during treatment. Current methods sterilize eggs from laboratory-reared blowfly colonies, after which sterile early second instar maggots can be provided to hospitals for use in treatment. Maggots not required for treatment are used for colony regeneration, in which sterility is not maintained...
April 12, 2017: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/28398264/factors-affecting-species-identifications-of-blow-fly-pupae-based-upon-chemical-profiles-and-multivariate-statistics
#4
William Kranz, Clinton Carroll, Darren A Dixon, John V Goodpaster, Christine J Picard
Alternative methods for the identification of species of blow fly pupae have been developed over the years that consist of the analyses of chemical profiles. However, the effect of biotic and abiotic factors that could influence the predictive manner for the tests have not been evaluated. The lipids of blowfly pupae (Cochliomyia macellaria, Lucilia cuprina, Lucilia sericata, and Phormia regina) were extracted in pentane, derivatized, and analyzed by total-vaporization solid phase microextraction gas chromatography-mass spectrometry (TV-SPME GC-MS)...
April 11, 2017: Insects
https://www.readbyqxmd.com/read/28374542/to-eat-or-get-heat-behavioral-trade-offs-between-thermoregulation-and-feeding-in-gregarious-necrophagous-larvae
#5
Jana Podhorna, Cindy Aubernon, Marie Borkovcova, Julien Boulay, Valery Hedouin, Damien Charabidze
The thermoregulation behavior of Lucilia sericata larvae (Diptera: Calliphoridae), a necrophagous species that feeds on vertebrate cadavers, was investigated. These larvae require high heat incomes to develop, and can elevate temperatures by forming large aggregates. We hypothesized that L. sericata larvae should continue to feed at temperatures up to 38°C, which can be reached inside larval masses. Thermal regulation behavior such as movement between a hot food spot and colder areas was also postulated. The hypotheses were tested by tracking for 1h the activity of single, starved third instar larvae in a Petri dish containing one food spot (FS) that was heated to a constant temperature of 25°C, 34°C or 38°C with an ambient temperature of 25°C...
April 4, 2017: Insect Science
https://www.readbyqxmd.com/read/28364721/effects-of-environmental-temperature-on-oviposition-behavior-in-three-blow-fly-species-of-forensic-importance
#6
Helen Ody, Mark T Bulling, Kate M Barnes
A number of factors are known to affect blow fly behavior with respect to oviposition. Current research indicates that temperature is the most significant factor. However temperature thresholds for oviposition in forensically important blow flies have not been well studied. Here, the oviposition behavior of three species of forensically important blow fly species (Calliphora vicina, Calliphora vomitoria and Lucilia sericata,) was studied under controlled laboratory conditions over a range of temperatures (10-40°C)...
March 14, 2017: Forensic Science International
https://www.readbyqxmd.com/read/28333104/spectral-signatures-of-immature-lucilia-sericata-meigen-diptera-calliphoridae
#7
Jodie-A Warren, T D Pulindu Ratnasekera, David A Campbell, Gail S Anderson
Hyperspectral remote sensing is an innovative technology with applications in many sciences and is a non-destructive method that may offer more precise aging within development stages. Hyperspectral reflectance measurements from the anterior, midsection, and posterior of Lucilia sericata (Meigen) larvae and pupae were conducted daily from samples of the developing insects beginning at second instar. Only midsection measurements were conducted on second instar larvae due to their size, to ensure that the measurement was not of reflective surroundings...
March 23, 2017: Insects
https://www.readbyqxmd.com/read/28235562/urate-oxidase-produced-by-lucilia-sericata-medical-maggots-is-localized-in-malpighian-tubes-and-facilitates-allantoin-production
#8
Andre Baumann, Marisa Skaljac, Rüdiger Lehmann, Andreas Vilcinskas, Zdenӗk Franta
Lucilia sericata maggots are the only species currently approved for maggot debridement therapy (MDT), an alternative treatment for chronic and recalcitrant wounds. Maggots promote wound debridement, disinfection and healing by producing a complex mixture of proteins, peptides and low-molecular-weight compounds in their secretions and excretions, but the individual components are not well characterized at the molecular level. Here we investigated the purine catabolism pathway in L. sericata, focusing on the production of allantoin by Urate Oxidase (UO), which is thought to promote wound healing...
February 22, 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28193269/how-flies-are-flirting-on-the-fly
#9
Courtney Eichorn, Michael Hrabar, Emma C Van Ryn, Bekka S Brodie, Adam J Blake, Gerhard Gries
BACKGROUND: Flies have some of the most elaborate visual systems in the Insecta, often featuring large, sexually dimorphic eyes with specialized "bright zones" that may have a functional role during mate-seeking behavior. The fast visual system of flies is considered to be an adaptation in support of their advanced flight abilities. Here, we show that the immense processing speed of the flies' photoreceptors plays a crucial role in mate recognition. RESULTS: Video-recording wing movements of abdomen-mounted common green bottle flies, Lucilia sericata, under direct light at 15,000 frames per second revealed that wing movements produce a single, reflected light flash per wing beat...
February 14, 2017: BMC Biology
https://www.readbyqxmd.com/read/28162132/wohlfahrtiimonas-chitiniclastica-current-insights-into-an-emerging-human-pathogen
#10
P Schröttner, W W Rudolph, U Damme, C Lotz, E Jacobs, F Gunzer
Since the first description of Wohlfahrtiimonas chitiniclastica in 2008, a number of well described case reports demonstrating its pathogenic role in humans have been published. Infections may be closely linked to flies, such as Wohlfahrtia magnifica, Lucilia sericata, Chrysomya megacephala or Musca domestica. These insects are potent vectors for the distribution of W. chitiniclastica causing local or systemic infections originating from wounds infested with fly larvae. However, other potential sources of transmission of W...
May 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28117913/quality-control-of-a-medicinal-larval-lucilia-sericata-debridement-device-based-on-released-gelatinase-activity
#11
S F Pickles, D I Pritchard
Lucilia sericata Meigen (Diptera: Calliphoridae) larvae are manufactured worldwide for the treatment of chronic wounds. Published research has confirmed that the primary clinical effect of the product, debridement (the degradation of non-viable wound tissue), is accomplished by a range of enzymes released by larvae during feeding. The quality assessment of larval activity is currently achieved during production using meat-based assays, which monitor insect growth and/or the reduction in substrate mass. To support this, the present authors developed a complementary radial diffusion enzymatic assay to produce a visual and measureable indication of the activity of larval alimentary products (LAP) collected under standardized conditions, against a gelatin substrate...
January 24, 2017: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/28019718/excretions-secretions-from-medicinal-larvae-lucilia-sericata-inhibit-complement-activation-by-two-mechanisms
#12
Tetsuro Tamura, Gwendolyn Cazander, Suzan H M Rooijakkers, Leendert A Trouw, Peter H Nibbering
Larvae of the blowfly Lucilia sericata facilitate wound healing by removing dead tissue and biofilms from non-healing and necrotic wounds. Another beneficial action of larvae and their excretions/secretions (ES) is down-regulation of excessive inflammation. As prolonged complement activation is key to excessive inflammation, the aim of this study was to elucidate the mechanisms underlying the anti-complement activities of ES. Results revealed that heat sensitive serine proteases in ES degrade multiple complement proteins in all steps of the three complement activation pathways...
December 26, 2016: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/27917052/the-blowflies-of-the-madeira-archipelago-species-diversity-distribution-and-identification-diptera-calliphoridaes-l
#13
Catarina Prado E Castro, Krzysztof Szpila, Anabel Martínez-Sánchez, Rego, Isamberto Silva, Artur R M Serrano, Mário Boieiro
Knowledge on the taxonomic diversity and distribution of blowflies from the Madeira Archipelago is updated. New and interesting findings are reported for poorly studied islands and islets of this archipelago, together with a brief analysis of the diversity of Macaronesian Calliphoridae s. l. Seven blowfly species were collected during this study, including the first records of Calliphora vicina Robineau-Desvoidy, 1830, Chrysomya albiceps (Wiedemann, 1819), Lucilia sericata (Meigen, 1826), Pollenia rudis (Fabricius, 1794) and Stomorhina lunata (Fabricius, 1805) from Porto Santo, and of Calliphora vicina, Lucilia sericata and Stomorhina lunata from Desertas Islands...
2016: ZooKeys
https://www.readbyqxmd.com/read/27876970/larvicidal-activity-of-camphor-and-lavender-oils-against-sheep-blowfly-luciliasericata-diptera-calliphoridae
#14
Hatem A Shalaby, Rabab M El Khateeb, Amira H El Namaky, Heba M Ashry, Omnia M Kandil, Sanaa K A Abou El Dobal
In this study, the essential oils of camphor and lavender were tested in vitro against the third instar larvae of Lucilia sericata for the first time, following dipping toxicity technique. The toxicity results revealed that L. sericata larvae were susceptible to the applied essential oils. Lavender oil was more effective than camphor in killing of L. sericata larvae. With 32 % concentration, the mortality percentages of larvae were 100 and 93.3 %, respectively. Light and scanning electron microscopic examinations were done to determine the cuticular changes of L...
December 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27868332/the-transcriptional-responses-of-cultured-wound-cells-to-the-excretions-and-secretions-of-medicinal-lucilia-sericata-larvae
#15
Priscila Dauros Singorenko, Roseanne Rosario, John A Windsor, Anthony R Phillips, Cherie Blenkiron
Maggots, through their excretions and secretions (ES), promote wound healing by removing necrotic tissue, counter bacterial infection, and activate wound associated cells. We investigated the effects of a physiological dose of maggot ES on four wound-associated cell types in vitro with Affymetrix gene expression arrays; keratinocytes, endothelial cells, fibroblasts and monocytes. Keratinocytes showed the fewest (n=5; p<0.05, fold-change ±2) and smallest fold-changes (up to 2.32x) in gene expression and conversely THP1 monocytes had the most (n=233) and greatest magnitude (up to 44...
November 21, 2016: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/27861898/a-preliminary-study-of-insect-succession-in-al-ahsaa-oasis-in-the-eastern-region-of-the-kingdom-of-saudi-arabia
#16
Essam A Shaalan, Zeinab A El-Moaty, Salaheldin Abdelsalam, Gail S Anderson
Rabbit carcass decomposition was examined in four seasons in Al-Ahsaa Oasis. Decomposition rate was significantly faster in summer compared with other seasons. Fourteen insect species from four orders and seven families were recorded: Calliphoridae, Muscidae, Sarcophagidae, Formicidae, Histeridae, Dermestidae, and Tenebrionidae. Most of the fly maggots collected were Calliphoridae with only one species from both Muscidae and Sarcophagidae. Maggots of Calliphora vicina (R.-D.) and Chrysomya albiceps (Wiedemann) colonized the carcasses in all seasons while both Ch...
January 2017: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/27853125/canine-wound-myiasis-caused-by-lucilia-sericata-diptera-calliphoridae-in-korea
#17
Seongjun Choe, Dongmin Lee, Hansol Park, Hyeong-Kyu Jeon, Hakhyun Kim, Ji-Houn Kang, Cha-Ho Jee, Keeseon S Eom
Myiasis is a relatively common infection of animals kept as pets, although only 1 case of canine myiasis has been described so far in the Republic of Korea. In the present study, we report an additional case of canine wound myiasis with identification of its causative agent, Lucilia sericata. An 8-year-old male Siberian husky dog was referred with anorexia, vomiting, and diarrhea to the Chungbuk National University Veterinary Medical Center, Cheongju-si (city), Chungcheongbuk-do (province), Korea in July 2013...
October 2016: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/27792719/-comparative-study-of-the-efficacy-of-larva-therapy-for-debridement-and-control-of-bacterial-burden-compared-to-surgical-debridement-and-topical-application-of-an-antimicrobial
#18
José Contreras-Ruiz, Adán Fuentes-Suárez, Sara Arroyo-Escalante, David Moncada-Barron, María Cristina Sosa-de-Martínez, Ernesto Maravilla-Franco, Judith Guadalupe Domínguez-Cherit
Maggot debridement therapy (MDT) is the use of medical grade maggots of the fly Lucilia sericata for wound debridement. Recent observations show that MDT decreases bacterial burden as well. Venous ulcers are the most commonly seen in wound clinics and require, besides adequate treatment of venous hypertension, proper wound bed preparation with debri dement of necrotic tissue and control of potential infections. To evaluate the efficacy of MDT in venous ulcers a randomized controlled trial was designed to compare MDT to surgical debridement and topical application of silver sulfadiazine (SSD) in 19 patients for 4 weeks...
October 2016: Gaceta Médica de México
https://www.readbyqxmd.com/read/27770452/cuticle-hydrolysis-in-four-medically-important-fly-species-by-enzymes-of-the-entomopathogenic-fungus-conidiobolus-coronatus
#19
M I Boguś, E Włóka, A Wrońska, A Kaczmarek, M Kazek, K Zalewska, M Ligęza-Żuber, M Gołębiowski
Entomopathogenic fungi infect insects via penetration through the cuticle, which varies remarkably in chemical composition across species and life stages. Fungal infection involves the production of enzymes that hydrolyse cuticular proteins, chitin and lipids. Host specificity is associated with fungus-cuticle interactions related to substrate utilization and resistance to host-specific inhibitors. The soil fungus Conidiobolus coronatus (Constantin) (Entomophthorales: Ancylistaceae) shows virulence against susceptible species...
October 22, 2016: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/27696133/first-record-of-larval-secretions-of-cochliomyia-macellaria-fabricius-1775-diptera-calliphoridae-inhibiting-the-growth-of-staphylococcus-aureus-and-pseudomonas-aeruginosa
#20
F S Masiero, M F K Aquino, M P Nassu, D I B Pereira, D S Leite, P J Thyssen
Maggot debridement therapy (MDT) consists on the intentional and controlled application of sterilized larvae of the order Diptera on necrotic skin lesions with the purpose of cleaning necrotic tissue and removing pathogenic bacteria. During MDT, a marked antimicrobial activity has been reported in literature specially associated with antibacterial substances from Lucilia sericata (Meigen); however, regarding Cochliomyia macellaria (Fabricius), little is known. This study aimed to evaluate in vitro inhibition of bacterial growth of Pseudomonas aeruginosa and Staphylococcus aureus in contact with excretions and secretions (ES) from C...
February 2017: Neotropical Entomology
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