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lucilia sericata

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https://www.readbyqxmd.com/read/29114146/immunological-evaluation-of-some-antigens-of-lucilia-sericata-larvae
#1
Hoda S Mohamed, Magdy M Fahmy, Marwa M Attia, Rabab M El Khateeb, Hatem A Shalaby, Mai A Mohamed
The present study aimed to select an antigen of Lucilia sericata larvae showing both high antigenicity and cross-reactive binding abilities with other related antigens of L. sericata larvae for obtaining a promising candidate vaccine antigen. The ELISA results primary concluded that among the excretory secretory (ES) and midgut (MG) antigens of the different larval instars of L. sericata, MGL2 could be characterized as antigen which was able to reflect the highest level of antigenicity and cross-reactivity with the other tested L...
December 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/29107219/adult-fly-age-estimations-using-cuticular-hydrocarbons-and-artificial-neural-networks-in-forensically-important-calliphoridae-species
#2
Hannah E Moore, John B Butcher, Charles R Day, Falko P Drijfhout
Blowflies (Diptera: Calliphoridae) are forensically important as they are known to be one of the first to colonise human remains. The larval stage is typically used to assist a forensic entomologists with adult flies rarely used as they are difficult to age because they remain morphologically similar once they have gone through the initial transformation upon hatching. However, being able to age them is of interest and importance within the field. This study examined the cuticular hydrocarbons (CHC) of Diptera: Calliphoridae species Lucilia sericata, Calliphora vicina and Calliphora vomitoria...
October 8, 2017: Forensic Science International
https://www.readbyqxmd.com/read/29040631/longevity-fertility-and-fecundity-of-adult-blow-flies-diptera-calliphoridae-held-at-varying-densities-implications-for-use-in-bioconversion-of-waste
#3
Nina Jennifer Parry, Elsje Pieterse, Christopher William Weldon
Large numbers of flies are needed to produce the quantity of larvae required for insect bioconversion of waste. However, this 'mass-rearing' may negatively affect adult survival and reproductive output. This study assessed the suitability for mass-rearing of four blow fly species, Chrysomya chloropyga, Chrysomya chloropyga (Wiedemann), Chrysomya megacephala (F.), Chrysomya putoria (Wiedemann) and Lucilia sericata (Meigen). Flies were kept at densities of 20, 50, 100, 250, 500, and 1,000 flies per 30 × 30 × 30 cm cage with an even sex ratio...
October 11, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28982577/evaluating-the-anti-leishmania-activity-of-lucilia-sericata-and-sarconesiopsis-magellanica-blowfly-larval-excretions-secretions-in-an-in-vitro-model
#4
Mayra Juliana Laverde-Paz, María Clara Echeverry, Manuel Alfonso Patarroyo, Felio Jesús Bello
Leishmaniasis is a vector-borne disease caused by infection by parasites from the genus Leishmania. Clinical manifestations can be visceral or cutaneous, the latter mainly being chronic ulcers. This work was aimed at evaluating Calliphoridae Lucilia sericata- and Sarconesiopsis magellanica-derived larval excretions and secretions' (ES) in vitro anti-leishmanial activity against Leishmania panamensis. Different larval-ES concentrations from both blowfly species were tested against either L. panamensis promastigotes or intracellular amastigotes using U937-macrophages as host cells...
October 2, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28979359/external-ophthalmomyiasis-caused-by-lucilia-sericata-diptera-challiphoride-larva
#5
Roghayeh Norouzi, Arman Manochehri, Saman Zarrin
Myiasis is an animal or human pathogenic condition initiated by parasitic dipterous fly larvae feeding in the host's necrotic or living tissue. Here we report a case of external ophthalmomyiasis caused by Lucilia sericata in a 78-yr-old with a vascular tumor of the retina and surgery history, from Bijar City of Kurdistan Province, Iran in 2015. Associated symptoms included right eye pain with mucoid ocular discharge, headache, sensing the presence of a foreign body in the eye and itching. Examination revealed a L...
July 2017: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28944889/beneficial-effects-of-extracts-from-lucilia%C3%A2-sericata-maggots-on-burn-wounds-in-rats
#6
Haixu Bian, Qiaoli Yang, Tao Ma, Wei Li, Jialin Duan, Guo Wei, Xiaoxiao Wu, Fei Mu, Rui Lin, Aidong Wen, Miaomiao Xi
Lucilia sericata maggots have beneficial properties; however, their protective effects on burn wounds have yet to be fully elucidated. In the present study, a deep second‑degree burn rat model was used to investigate the burn wound healing properties of aqueous extract of maggots (MAE). The anti‑inflammatory, antioxidative and antibacterial activities were examined. In addition, the protein expression levels of Akt, vascular endothelial growth factor A (VEGFA) and nuclear factor‑κB (NF‑κB) were detected by western blotting...
November 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28889865/estimating-the-age-of-the-adult-stages-of-the-blow-flies-lucilia-sericata-and-calliphora-vicina-diptera-calliphoridae-by-means-of-the-cuticular-hydrocarbon-n-pentacosane
#7
Victoria Bernhardt, Werner Pogoda, Marcel A Verhoff, Stefan W Toennes, Jens Amendt
Age estimation of insects like blow flies plays an important role in forensic entomology and can answer questions in regard to time of death. So far the focus is on the immature stages of these insects, but recently the adult fly became a target of interest. It has been established that the profile of specific cuticular hydrocarbons (CHCs) changes in a consistent pattern as adult insects age; thus, their analysis could be a promising tool for the age estimation of adult insects. We investigated the CHC n-pentacosane (nC25) on the legs of the adult blow flies Lucilia sericata and Calliphora vicina with gas chromatography-mass spectrometry...
September 2017: Science & Justice: Journal of the Forensic Science Society
https://www.readbyqxmd.com/read/28803354/facultative-myiasis-of-domestic-cats-by-sarcophaga-argyrostoma-diptera-sarcophagidae-calliphora-vicina-and-lucilia-sericata-diptera-calliphoridae-in-northern-italy
#8
Marco Pezzi, Daniel Whitmore, Teresa Bonacci, Carlo Nicola Francesco Del Zingaro, Milvia Chicca, Massimo Lanfredi, Marilena Leis
We describe five cases of myiasis of domestic cats, Felis silvestris catus L. (Carnivora: Felidae), reported in 2016 in northern Italy and caused by three Diptera species: Sarcophaga argyrostoma (Robineau-Desvoidy) (Sarcophagidae), Calliphora vicina Robineau-Desvoidy and Lucilia sericata (Meigen) (Calliphoridae). Three were cases of traumatic myiasis, one by S. argyrostoma and two by L. sericata, one was a case of auricular myiasis by C. vicina and one was a case of ophthalmomyiasis caused by an association of L...
October 2017: Parasitology Research
https://www.readbyqxmd.com/read/28762629/evaluation-of-blowfly-larvae-diptera-calliphoridae-as-possible-reservoirs-and-mechanical-vectors-of-african-swine-fever-virus
#9
J H Forth, J Amendt, S Blome, K Depner, H Kampen
In 2014, highly virulent African swine fever virus (ASFV) was introduced into the Baltic States and Poland, with new cases being reported almost every week from wild boar and also from domestic pigs. Contrary to initial predictions that the disease would either die out due to the high virulence of the virus strain or spread rapidly in westerly direction, the infection became endemic and spread slowly. The unexpected disease epidemiology led to the hypothesis that hitherto unconsidered factors might contribute to virus persistence and dispersal...
August 1, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/28710939/evaluation-of-bait-traps-as-a-means-to-predict-initial-blow-fly-diptera-calliphoridae-communities-associated-with-decomposing-swine-remains-in-new-jersey-usa
#10
Lauren M Weidner, M Denise Gemmellaro, Jeffery K Tomberlin, George C Hamilton
Information about blow fly (Diptera: Calliphoridae) species distributions can be valuable for criminal investigations, with regards to determining movement of remains from one location to another and time of colonization estimates, making these data extremely useful. Past work has been conducted on initial species community structure across New Jersey, USA using traps baited with beef liver; however, if these same species frequent vertebrate carrion remains unclear. In order to evaluate these data, piglet carcasses were placed out once every two weeks for a year in New Brunswick, New Jersey, USA...
September 2017: Forensic Science International
https://www.readbyqxmd.com/read/28708988/early-colonisation-of-urban-indoor-carcasses-by-blow-flies-diptera-calliphoridae-an-experimental-study-from-central-spain
#11
Daniel Martín-Vega, Carla Martín Nieto, Blanca Cifrián, Arturo Baz, Luisa M Díaz-Aranda
Due to their ubiquity and synanthropy, blow flies (Diptera: Calliphoridae) are generally the first colonisers of cadavers and, therefore, frequently used to estimate a minimum post-mortem interval (minPMI). Whereas in outdoor situations blow flies are expected to locate and colonise exposed cadavers within hours or even minutes after death, it is usually assumed that the colonisation of a cadaver indoors might be delayed for an uncertain period of time. This uncertainty severely limits the informativity of minPMI estimates based on entomological evidence...
September 2017: Forensic Science International
https://www.readbyqxmd.com/read/28695964/segmentation-gene-expression-patterns-in-bactrocera-dorsalis-and-related-insects-regulation-and-shape-of-blastoderm-and-larval-cuticle
#12
Worramin Suksuwan, Xiaoli Cai, Lertluk Ngernsiri, Stefan Baumgartner
The oriental fruit fly, Bactrocera dorsalis, is regarded as a severe pest of fruit production in Asia. Despite its economic importance, only limited information regarding the molecular and developmental biology of this insect is known to date. We provide a detailed analysis of B. dorsalis embryology, as well as the expression patterns of a number of segmentation genes known to act during patterning of Drosophila and compare these to the patterns of other insect families. An anterior shift of the expression of gap genes was detected when compared to Drosophila...
2017: International Journal of Developmental Biology
https://www.readbyqxmd.com/read/28668988/blowfly-puparia-in-a-hermetic-container-survival-under-decreasing-oxygen-conditions
#13
Anna Mądra-Bielewicz, Katarzyna Frątczak-Łagiewska, Szymon Matuszewski
Despite widely accepted standards for sampling and preservation of insect evidence, unrepresentative samples or improperly preserved evidence are encountered frequently in forensic investigations. Here, we report the results of laboratory studies on the survival of Lucilia sericata and Calliphora vomitoria (Diptera: Calliphoridae) intra-puparial forms in hermetic containers, which were stimulated by a recent case. It is demonstrated that the survival of blowfly intra-puparial forms inside airtight containers is dependent on container volume, number of puparia inside, and their age...
July 1, 2017: Forensic Science, Medicine, and Pathology
https://www.readbyqxmd.com/read/28621210/effects-of-lucilia-sericata-on-wound-healing-in-streptozotocin-induced-diabetic-rats-and-analysis-of-its-secretome-at-the-proteome-level
#14
F K Tombulturk, M Kasap, M Tuncdemir, E Polat, S Sirekbasan, A Kanli, G Kanigur-Sultuybek
The use of Lucilia sericata larvae on the healing of wounds in diabetics has been reported. However, the role of the excretion/secretion (ES) products of the larvae in treatment of diabetic wounds remains unknown. This study investigated whether application of the ES products of L. sericata on the wound surface could improve the impaired wound healing in streptozotocin-induced diabetic rats. Additional analysis was performed to understand proteome content of L. sericata secretome to understand ES contribution at the molecular level...
January 1, 2017: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/28586212/species-identification-of-necrophagous-insect-eggs-based-on-amino-acid-profile-differences-revealed-by-direct-analysis-in-real-time-high-resolution-mass-spectrometry
#15
Justine E Giffen, Jennifer Y Rosati, Cameron M Longo, Rabi A Musah
The colonization of decomposing remains by necrophagous insects such as blow flies is of forensic importance because the progression through the various stages of insect development can be correlated to time of death. The ability to infer this information hinges on accurate determination of the fly species that are associated with the entomological evidence collected. This evidence can include eggs, larvae, pupae, and puparial casings. Determination of the egg's identity is particularly challenging because the eggs of multiple species are morphologically very similar...
July 3, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28567525/forensic-entomotoxicology-revisited-towards-professional-standardisation-of-study-designs
#16
REVIEW
Erica I T da Silva, Brendan Wilhelmi, Martin H Villet
Forensic entomotoxicology is the use of insects as evidence of whether a toxicant is present in an environment such as a corpse, river or landscape. The earliest overtly forensic study was published in 1977, and since then, at least 63 papers have been published, most of them focused on the detection of toxicants in insects or on effects of toxicants on diverse insect indicator taxa. A comprehensive review of the published literature revealed various inconsistencies between studies that could be addressed by introducing standard protocols for such studies...
May 31, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28512294/the-potential-use-of-cuticular-hydrocarbons-and-multivariate-analysis-to-age-empty-puparial-cases-of-calliphora-vicina-and-lucilia-sericata
#17
Hannah E Moore, Jennifer L Pechal, M Eric Benbow, Falko P Drijfhout
Cuticular hydrocarbons (CHC) have been successfully used in the field of forensic entomology for identifying and ageing forensically important blowfly species, primarily in the larval stages. However in older scenes where all other entomological evidence is no longer present, Calliphoridae puparial cases can often be all that remains and therefore being able to establish the age could give an indication of the PMI. This paper examined the CHCs present in the lipid wax layer of insects, to determine the age of the cases over a period of nine months...
May 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28474172/age-estimation-during-the-blow-fly-intra-puparial-period-a-qualitative-and-quantitative-approach-using-micro-computed-tomography
#18
Daniel Martín-Vega, Thomas J Simonsen, Martina Wicklein, Martin J R Hall
Minimum post-mortem interval (minPMI) estimates often rely on the use of developmental data from blow flies (Diptera: Calliphoridae), which are generally the first colonisers of cadavers and, therefore, exemplar forensic indicators. Developmental data of the intra-puparial period are of particular importance, as it can account for more than half of the developmental duration of the blow fly life cycle. During this period, the insect undergoes metamorphosis inside the opaque, barrel-shaped puparium, formed by the hardening and darkening of the third instar larval cuticle, which shows virtually no external changes until adult emergence...
May 4, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28472319/evaluation-of-sterilized-artificial-diets-for-mass-rearing-the-lucilia-sericata-diptera-calliphoridae
#19
COMPARATIVE STUDY
Le Zheng, Tawni L Crippen, Alan Dabney, Alex Gordy, Jeffery K Tomberlin
The impact of six sterilized diets (blood-yeast agar diet, decomposed beef liver diet, powdered beef liver diet, powdered fish diet, milk-based diet, and a chemically defined diet) on Lucilia sericata (Meigen) larvae reared at three densities (10 larvae, 20 larvae, and 40 larvae on 20 g diet) was determined in comparison to fresh beef liver as a control. Specifically, the effects of these diets on the following traits of L. sericata were measured: 1) pupal weight, 2) pupation percentage, 3) eclosion percentage, as well as 4) adult longevity...
September 1, 2017: Journal of Medical Entomology
https://www.readbyqxmd.com/read/28454623/effects-of-different-storage-and-measuring-methods-on-larval-length-values-for-the-blow-flies-diptera-calliphoridae-lucilia-sericata-and-calliphora-vicina
#20
Valentina Bugelli, Carlo Pietro Campobasso, Marcel A Verhoff, Jens Amendt
In forensic entomology, the methods of sampling, killing, and storing entomological samples can affect larval age estimation, and, hence, the estimation of the minimum post-mortem interval. In the existing manuals, there is a certain amount of heterogeneity regarding methods and the recommendations for best practice in forensic entomology are insufficiently validated. This study evaluated three different length-measurement methods for larval stages and examined the influence of different killing and storing methods on the larval length of two forensically important blow flies, Lucilia sericata and Calliphora vicina...
May 2017: Science & Justice: Journal of the Forensic Science Society
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