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lucilia sericata

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https://www.readbyqxmd.com/read/29324424/larval-therapy-for-chronic-cutaneous-ulcers-historical-review-and-future-perspectives
#1
Edoardo Raposio, Sara Bortolini, Lara Maistrello, Donato A Grasso
Cutaneous ulcers tend to become chronic and have a profound impact on quality of life. These wounds may become infected and lead to greater morbidity and even mortality. In the past, larvae (ie, maggots) of certain common flies (Lucilia sericata and Lucilia cuprina) were considered useful in ulcer management because they only remove necrotic tissue while promoting healthy tissue in the wound bed, thus helping wounds heal faster. Recently, maggots from several other fly species (Calliphora vicina, Calliphora vomitoria, Phormia regina, Chrysomya albiceps, Sarcophaga carnaria, and Hermetia illucens) have been shown in vitro to possess characteristics (ie, debridement efficacy and putative antimicrobial potentialities) that make them suitable candidates for possible use in clinical practice...
December 2017: Wounds: a Compendium of Clinical Research and Practice
https://www.readbyqxmd.com/read/29322053/insect-fauna-of-human-cadavers-in-tehran-district
#2
Fahimeh Talebzadeh, Masoud Ghadipasha, Jaber Gharedaghi, Naser Yeksan, Kamran Akbarzadeh, Mohammad Ali Oshaghi
Background: Entomological data can provide valuable information for crime scene investigations especially in post-mortem interval (PMI) estimation. This study performed to determine insect fauna of human corpses in Tehran district. Methods: Insect specimens were collected from 12 human cadavers during spring and summer 2014 and were identified using morphological characteristics. Results: Four fly species including two blowflies Chrysomya albiceps and Lucilia sericata (Calliphoridae), one flesh fly Sarcophaga argyrostoma (Sarcophagidae), and one phorid fly Megaselia scalaris (Phoridae) and a beetle Dermestes maculatus (Dermestidae) was observed on the human cadavers...
September 2017: Journal of Arthropod-borne Diseases
https://www.readbyqxmd.com/read/29244970/maggot-debridement-therapy-for-an-electrical-burn-injury-with-instructions-for-the-use-of-lucilia-sericata-larvae
#3
A Nasoori, R Hoomand
OBJECTIVE: Maggot debridement therapy (MDT) has proved an efficient and reliable approach for wound healing. We explain the use of maggots with a stepwise procedure for conducting MDT in a patient with severe and large electrical wounds. METHOD: We captured the flies of Lucilia sericata from the natural environment, Alborz, Iran, and reared them in netted cages. The eggs obtained from the flies were disinfected with chloramine, and then were incubated on culture medium...
December 2, 2017: Journal of Wound Care
https://www.readbyqxmd.com/read/29189911/same-same-but-different-matching-entomological-traces-to-a-human-food-source-by-stable-isotope-analysis
#4
Victoria Bernhardt, Thomas Holdermann, Nicole Scheid, Thomas Schäfer, Marcel A Verhoff, Jens Amendt
Blow flies are important tools in forensic entomology, as they feed and develop on human bodies. This fact can be used to estimate the minimum post mortem interval (PMImin), by classifying the age of the immature stages found on corpses. The capacity to also be able to classify the age of adult flies, or even empty puparia, could increase the timeframe for a PMImin. However, the association of older developmental stages, such as adult flies or their empty cases, with a human cadaver may be challenged, as such specimens could stem from another food source...
November 30, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/29114146/immunological-evaluation-of-some-antigens-of-lucilia-sericata-larvae
#5
Hoda S Mohamed, Magdy M Fahmy, Marwa M Attia, Rabab M El Khateeb, Hatem A Shalaby, Mai A Mohamed
The present study aimed to select an antigen of Lucilia sericata larvae showing both high antigenicity and cross-reactive binding abilities with other related antigens of L. sericata larvae for obtaining a promising candidate vaccine antigen. The ELISA results primary concluded that among the excretory secretory (ES) and midgut (MG) antigens of the different larval instars of L. sericata, MGL2 could be characterized as antigen which was able to reflect the highest level of antigenicity and cross-reactivity with the other tested L...
December 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/29107219/adult-fly-age-estimations-using-cuticular-hydrocarbons-and-artificial-neural-networks-in-forensically-important-calliphoridae-species
#6
Hannah E Moore, John B Butcher, Charles R Day, Falko P Drijfhout
Blowflies (Diptera: Calliphoridae) are forensically important as they are known to be one of the first to colonise human remains. The larval stage is typically used to assist a forensic entomologists with adult flies rarely used as they are difficult to age because they remain morphologically similar once they have gone through the initial transformation upon hatching. However, being able to age them is of interest and importance within the field. This study examined the cuticular hydrocarbons (CHC) of Diptera: Calliphoridae species Lucilia sericata, Calliphora vicina and Calliphora vomitoria...
October 8, 2017: Forensic Science International
https://www.readbyqxmd.com/read/29040631/longevity-fertility-and-fecundity-of-adult-blow-flies-diptera-calliphoridae-held-at-varying-densities-implications-for-use-in-bioconversion-of-waste
#7
Nina Jennifer Parry, Elsje Pieterse, Christopher William Weldon
Large numbers of flies are needed to produce the quantity of larvae required for insect bioconversion of waste. However, this 'mass-rearing' may negatively affect adult survival and reproductive output. This study assessed the suitability for mass-rearing of four blow fly species, Chrysomya chloropyga, Chrysomya chloropyga (Wiedemann), Chrysomya megacephala (F.), Chrysomya putoria (Wiedemann) and Lucilia sericata (Meigen). Flies were kept at densities of 20, 50, 100, 250, 500, and 1,000 flies per 30 × 30 × 30 cm cage with an even sex ratio...
October 11, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28982577/evaluating-the-anti-leishmania-activity-of-lucilia-sericata-and-sarconesiopsis-magellanica-blowfly-larval-excretions-secretions-in-an-in-vitro-model
#8
Mayra Juliana Laverde-Paz, María Clara Echeverry, Manuel Alfonso Patarroyo, Felio Jesús Bello
Leishmaniasis is a vector-borne disease caused by infection by parasites from the genus Leishmania. Clinical manifestations can be visceral or cutaneous, the latter mainly being chronic ulcers. This work was aimed at evaluating Calliphoridae Lucilia sericata- and Sarconesiopsis magellanica-derived larval excretions and secretions' (ES) in vitro anti-leishmanial activity against Leishmania panamensis. Different larval-ES concentrations from both blowfly species were tested against either L. panamensis promastigotes or intracellular amastigotes using U937-macrophages as host cells...
October 2, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28979359/external-ophthalmomyiasis-caused-by-lucilia-sericata-diptera-challiphoride-larva
#9
Roghayeh Norouzi, Arman Manochehri, Saman Zarrin
Myiasis is an animal or human pathogenic condition initiated by parasitic dipterous fly larvae feeding in the host's necrotic or living tissue. Here we report a case of external ophthalmomyiasis caused by Lucilia sericata in a 78-yr-old with a vascular tumor of the retina and surgery history, from Bijar City of Kurdistan Province, Iran in 2015. Associated symptoms included right eye pain with mucoid ocular discharge, headache, sensing the presence of a foreign body in the eye and itching. Examination revealed a L...
July 2017: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28944889/beneficial-effects-of-extracts-from-lucilia%C3%A2-sericata-maggots-on-burn-wounds-in-rats
#10
Haixu Bian, Qiaoli Yang, Tao Ma, Wei Li, Jialin Duan, Guo Wei, Xiaoxiao Wu, Fei Mu, Rui Lin, Aidong Wen, Miaomiao Xi
Lucilia sericata maggots have beneficial properties; however, their protective effects on burn wounds have yet to be fully elucidated. In the present study, a deep second‑degree burn rat model was used to investigate the burn wound healing properties of aqueous extract of maggots (MAE). The anti‑inflammatory, antioxidative and antibacterial activities were examined. In addition, the protein expression levels of Akt, vascular endothelial growth factor A (VEGFA) and nuclear factor‑κB (NF‑κB) were detected by western blotting...
November 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28889865/estimating-the-age-of-the-adult-stages-of-the-blow-flies-lucilia-sericata-and-calliphora-vicina-diptera-calliphoridae-by-means-of-the-cuticular-hydrocarbon-n-pentacosane
#11
Victoria Bernhardt, Werner Pogoda, Marcel A Verhoff, Stefan W Toennes, Jens Amendt
Age estimation of insects like blow flies plays an important role in forensic entomology and can answer questions in regard to time of death. So far the focus is on the immature stages of these insects, but recently the adult fly became a target of interest. It has been established that the profile of specific cuticular hydrocarbons (CHCs) changes in a consistent pattern as adult insects age; thus, their analysis could be a promising tool for the age estimation of adult insects. We investigated the CHC n-pentacosane (nC25) on the legs of the adult blow flies Lucilia sericata and Calliphora vicina with gas chromatography-mass spectrometry...
September 2017: Science & Justice: Journal of the Forensic Science Society
https://www.readbyqxmd.com/read/28803354/facultative-myiasis-of-domestic-cats-by-sarcophaga-argyrostoma-diptera-sarcophagidae-calliphora-vicina-and-lucilia-sericata-diptera-calliphoridae-in-northern-italy
#12
Marco Pezzi, Daniel Whitmore, Teresa Bonacci, Carlo Nicola Francesco Del Zingaro, Milvia Chicca, Massimo Lanfredi, Marilena Leis
We describe five cases of myiasis of domestic cats, Felis silvestris catus L. (Carnivora: Felidae), reported in 2016 in northern Italy and caused by three Diptera species: Sarcophaga argyrostoma (Robineau-Desvoidy) (Sarcophagidae), Calliphora vicina Robineau-Desvoidy and Lucilia sericata (Meigen) (Calliphoridae). Three were cases of traumatic myiasis, one by S. argyrostoma and two by L. sericata, one was a case of auricular myiasis by C. vicina and one was a case of ophthalmomyiasis caused by an association of L...
October 2017: Parasitology Research
https://www.readbyqxmd.com/read/28762629/evaluation-of-blowfly-larvae-diptera-calliphoridae-as-possible-reservoirs-and-mechanical-vectors-of-african-swine-fever-virus
#13
J H Forth, J Amendt, S Blome, K Depner, H Kampen
In 2014, highly virulent African swine fever virus (ASFV) was introduced into the Baltic States and Poland, with new cases being reported almost every week from wild boar and also from domestic pigs. Contrary to initial predictions that the disease would either die out due to the high virulence of the virus strain or spread rapidly in westerly direction, the infection became endemic and spread slowly. The unexpected disease epidemiology led to the hypothesis that hitherto unconsidered factors might contribute to virus persistence and dispersal...
August 1, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/28710939/evaluation-of-bait-traps-as-a-means-to-predict-initial-blow-fly-diptera-calliphoridae-communities-associated-with-decomposing-swine-remains-in-new-jersey-usa
#14
Lauren M Weidner, M Denise Gemmellaro, Jeffery K Tomberlin, George C Hamilton
Information about blow fly (Diptera: Calliphoridae) species distributions can be valuable for criminal investigations, with regards to determining movement of remains from one location to another and time of colonization estimates, making these data extremely useful. Past work has been conducted on initial species community structure across New Jersey, USA using traps baited with beef liver; however, if these same species frequent vertebrate carrion remains unclear. In order to evaluate these data, piglet carcasses were placed out once every two weeks for a year in New Brunswick, New Jersey, USA...
September 2017: Forensic Science International
https://www.readbyqxmd.com/read/28708988/early-colonisation-of-urban-indoor-carcasses-by-blow-flies-diptera-calliphoridae-an-experimental-study-from-central-spain
#15
Daniel Martín-Vega, Carla Martín Nieto, Blanca Cifrián, Arturo Baz, Luisa M Díaz-Aranda
Due to their ubiquity and synanthropy, blow flies (Diptera: Calliphoridae) are generally the first colonisers of cadavers and, therefore, frequently used to estimate a minimum post-mortem interval (minPMI). Whereas in outdoor situations blow flies are expected to locate and colonise exposed cadavers within hours or even minutes after death, it is usually assumed that the colonisation of a cadaver indoors might be delayed for an uncertain period of time. This uncertainty severely limits the informativity of minPMI estimates based on entomological evidence...
September 2017: Forensic Science International
https://www.readbyqxmd.com/read/28695964/segmentation-gene-expression-patterns-in-bactrocera-dorsalis-and-related-insects-regulation-and-shape-of-blastoderm-and-larval-cuticle
#16
Worramin Suksuwan, Xiaoli Cai, Lertluk Ngernsiri, Stefan Baumgartner
The oriental fruit fly, Bactrocera dorsalis, is regarded as a severe pest of fruit production in Asia. Despite its economic importance, only limited information regarding the molecular and developmental biology of this insect is known to date. We provide a detailed analysis of B. dorsalis embryology, as well as the expression patterns of a number of segmentation genes known to act during patterning of Drosophila and compare these to the patterns of other insect families. An anterior shift of the expression of gap genes was detected when compared to Drosophila...
2017: International Journal of Developmental Biology
https://www.readbyqxmd.com/read/28668988/blowfly-puparia-in-a-hermetic-container-survival-under-decreasing-oxygen-conditions
#17
Anna Mądra-Bielewicz, Katarzyna Frątczak-Łagiewska, Szymon Matuszewski
Despite widely accepted standards for sampling and preservation of insect evidence, unrepresentative samples or improperly preserved evidence are encountered frequently in forensic investigations. Here, we report the results of laboratory studies on the survival of Lucilia sericata and Calliphora vomitoria (Diptera: Calliphoridae) intra-puparial forms in hermetic containers, which were stimulated by a recent case. It is demonstrated that the survival of blowfly intra-puparial forms inside airtight containers is dependent on container volume, number of puparia inside, and their age...
July 1, 2017: Forensic Science, Medicine, and Pathology
https://www.readbyqxmd.com/read/28621210/effects-of-lucilia-sericata-on-wound-healing-in-streptozotocin-induced-diabetic-rats-and-analysis-of-its-secretome-at-the-proteome-level
#18
F K Tombulturk, M Kasap, M Tuncdemir, E Polat, S Sirekbasan, A Kanli, G Kanigur-Sultuybek
The use of Lucilia sericata larvae on the healing of wounds in diabetics has been reported. However, the role of the excretion/secretion (ES) products of the larvae in treatment of diabetic wounds remains unknown. This study investigated whether application of the ES products of L. sericata on the wound surface could improve the impaired wound healing in streptozotocin-induced diabetic rats. Additional analysis was performed to understand proteome content of L. sericata secretome to understand ES contribution at the molecular level...
January 1, 2017: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/28586212/species-identification-of-necrophagous-insect-eggs-based-on-amino-acid-profile-differences-revealed-by-direct-analysis-in-real-time-high-resolution-mass-spectrometry
#19
Justine E Giffen, Jennifer Y Rosati, Cameron M Longo, Rabi A Musah
The colonization of decomposing remains by necrophagous insects such as blow flies is of forensic importance because the progression through the various stages of insect development can be correlated to time of death. The ability to infer this information hinges on accurate determination of the fly species that are associated with the entomological evidence collected. This evidence can include eggs, larvae, pupae, and puparial casings. Determination of the egg's identity is particularly challenging because the eggs of multiple species are morphologically very similar...
July 3, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28567525/forensic-entomotoxicology-revisited-towards-professional-standardisation-of-study-designs
#20
REVIEW
Erica I T da Silva, Brendan Wilhelmi, Martin H Villet
Forensic entomotoxicology is the use of insects as evidence of whether a toxicant is present in an environment such as a corpse, river or landscape. The earliest overtly forensic study was published in 1977, and since then, at least 63 papers have been published, most of them focused on the detection of toxicants in insects or on effects of toxicants on diverse insect indicator taxa. A comprehensive review of the published literature revealed various inconsistencies between studies that could be addressed by introducing standard protocols for such studies...
May 31, 2017: International Journal of Legal Medicine
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