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sarcoplasmic reticulum ca2+ atpase

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https://www.readbyqxmd.com/read/27737323/thyroxine-increases-serca2-and-ryr2-gene-expression-in-heart-failure-rats-with-euthyroid-sick-syndrome
#1
Fábio V G Campanha, Denise Perone, Dijon H S de Campos, Renata de A M Luvizotto, Maria T De Síbio, Miriane de Oliveira, Regiane M C Olimpio, Fernanda C F Moretto, Carlos R Padovani, Gláucia M F S Mazeto, Antonio C Cicogna, Célia R Nogueira
Objective: The current study was aimed at analyzing sarcoplasmic reticulum Ca2+ ATPase (Serca2) and ryanodine receptor type 2 (Ryr2) gene expression in rats subjected to surgery that induced HF and were subsequently treated with T4 using physiological doses. Materials and methods: HF was induced in 18 male Wistar rats by clipping the ascending thoracic aorta to generate aortic stenosis (HFS group), while the control group (9-sham) underwent thoracotomy. After 21 weeks, the HFS group was subdivided into two subgroups...
October 10, 2016: Archives of Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27641420/gastrodin-pretreatment-impact-on-sarcoplasmic-reticulum-calcium-transport-atpase-serca-and-calcium-phosphate-plb-expression-in-rats-with-myocardial-ischemia-reperfusion
#2
Yufen Li, Xiaomei Wang, Changli Lou
BACKGROUND Calcium overload, inflammation, and apoptosis play important roles in myocardial ischemia-reperfusion injury (MIRI). Gastrodin pretreatment can alleviate MIRI. This study observed sarcoplasmic reticulum calcium transport ATPase (Ca2+-ATPase, SERCA) and calcium phosphate (PLB) protein expression in the ventricular remodeling process after myocardial infarction to explore the effect of gastrodin pretreatment on MIRI. MATERIAL AND METHODS Healthy 7-week-old male SD rats were randomly divided into a sham group (A), a model group (B), and gastrodin pretreatment groups C, D, and E (100, 200, and 400 mg/kg, respectively) with 20 in each group...
September 19, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/27591219/resveratrol-protects-against-lipopolysaccharide-induced-cardiac-dysfunction-by-enhancing-serca2a-activity-through-promoting-the-phospholamban-oligomerization
#3
Tao Bai, Xinyue Hu, Yang Zheng, Shudong Wang, Jian Kong, Lu Cai
The bacterial endotoxin lipopolysaccharide (LPS) is a main culprit responsible for cardiac dysfunction in sepsis. This study examined whether resveratrol could protect against LPS-induced cardiac dysfunction by improving the sarcoplasmic endoplasmic reticulum Ca(2+)-ATPase (SERCA2a) activity. Echocardiographic parameters, cardiomyocyte contractile and Ca(2+) transient properties, markers for cardiac inflammation, cell death, and oxidative stress, SERCA2a activity, and the ratios of phospholamban (PLB) monomer to oligomer were measured...
October 1, 2016: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/27533258/jabuticaba-induced-endothelium-independent-vasodilating-effect-on-isolated-arteries
#4
Daniela Medeiros Lobo de Andrade, Leonardo Luis Borges, Ieda Maria Sapateiro Torres, Edemilson Cardoso da Conceição, Matheus Lavorenti Rocha
Background: Despite the important biological effects of jabuticaba, its actions on the cardiovascular system have not been clarified. Objectives: To determine the effects of jabuticaba hydroalcoholic extract (JHE) on vascular smooth muscle (VSM) of isolated arteries. Methods: Endothelium-denuded aortic rings of rats were mounted in isolated organ bath to record isometric tension. The relaxant effect of JHE and the influence of K+ channels and Ca2+ intra- and extracellular sources on JHE-stimulated response were assessed...
September 2016: Arquivos Brasileiros de Cardiologia
https://www.readbyqxmd.com/read/27501399/assessment-of-myofilament-ca2-sensitivity-underlying-cardiac-excitation-contraction-coupling
#5
Zai Hao Zhao, Chun Li Jin, Ji Hyun Jang, Yu Na Wu, Sung Joon Kim, Hong Hua Jin, Lan Cui, Yin Hua Zhang
Heart failure and cardiac arrhythmias are the leading causes of mortality and morbidity worldwide. However, the mechanism of pathogenesis and myocardial malfunction in the diseased heart remains to be fully clarified. Recent compelling evidence demonstrates that changes in the myofilament Ca(2+) sensitivity affect intracellular Ca(2+) homeostasis and ion channel activities in cardiac myocytes, the essential mechanisms responsible for the cardiac action potential and contraction in healthy and diseased hearts...
2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27269924/-effects-of-hydrogen-sulfide-on-contraction-capacity-of-diaphragm-from-type-1-diabetic-rats
#6
Qiang Jia, Shanfeng Ma, Xiaofen Liu, Sai Li, Yuanyuan Wang, Qin Gao, Rui Yang
OBJECTIVE: To investigate the effects of hydrogen sulfide (H2S) on contraction capacity of diaphragm in type 1 diabetic rats.
 METHODS: Thirty-two male SD rats were randomly divided into a normal group (NC), a diabetic group (DM), a NaHS treatment group (DM+NaHS) and a NaHS group (NaHS) (n=8). Intraperitoneal injection of streptozotocin was utilized to establish diabetic rat model. After the modeling, the rats in the DM+NaHS and the NaHS groups were intraperitoneally injected with 28 μmol/kg NaHS solution...
May 2016: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/27235390/k201-jtv519-is-a-ca2-dependent-blocker-of-serca-and-a-partial-agonist-of-ryanodine-receptors-in-striated-muscle
#7
Yuanzhao L Darcy, Paula L Diaz-Sylvester, Julio A Copello
K201 (JTV-519) may prevent abnormal Ca(2+) leak from the sarcoplasmic reticulum (SR) in the ischemic heart and skeletal muscle (SkM) by stabilizing the ryanodine receptors (RyRs; RyR1 and RyR2, respectively). We tested direct modulation of the SR Ca(2+)-stimulated ATPase (SERCA) and RyRs by K201. In isolated cardiac and SkM SR microsomes, K201 slowed the rate of SR Ca(2+) loading, suggesting potential SERCA block and/or RyR agonism. K201 displayed Ca(2+)-dependent inhibition of SERCA-dependent ATPase activity, which was measured in microsomes incubated with 200, 2, and 0...
August 2016: Molecular Pharmacology
https://www.readbyqxmd.com/read/27174327/the-involvement-of-ca-2-signal-pathways-in-distal-colonic-myocytes-in-a-rat-model-of-dextran-sulfate-sodium-induced-colitis
#8
Yan Wang, Jun-Xia Li, Guang-Ju Ji, Kui Zhai, Hua-Hong Wang, Xin-Guang Liu
BACKGROUND: Disrupted Ca2+ homeostasis contributes to the development of colonic dysmotility in ulcerative colitis (UC), but the underlying mechanisms are unknown. This study aimed to examine the alteration of colonic smooth muscle (SM) Ca2+ signaling and Ca2+ handling proteins in a rat model of dextran sulfate sodium (DSS)-induced UC. METHODS: Male Sprague-Dawley rats were randomly divided into control (n = 18) and DSS (n = 17) groups. Acute colitis was induced by 5% DSS in the drinking water for 7 days...
May 20, 2016: Chinese Medical Journal
https://www.readbyqxmd.com/read/27055500/calpain-3-deficiency-affects-serca-expression-and-function-in-the-skeletal-muscle
#9
Ivan Toral-Ojeda, Garazi Aldanondo, Jaione Lasa-Elgarresta, Haizpea Lasa-Fernández, Roberto Fernández-Torrón, Adolfo López de Munain, Ainara Vallejo-Illarramendi
Limb-girdle muscular dystrophy type 2A (LGMD2A) is a form of muscular dystrophy caused by mutations in calpain 3 (CAPN3). Several studies have implicated Ca2+ dysregulation as an underlying event in several muscular dystrophies, including LGMD2A. In this study we used mouse and human myotube cultures, and muscle biopsies in order to determine whether dysfunction of sarco/endoplasmatic Ca2+-ATPase (SERCA) is involved in the pathology of this disease. In CAPN3-deficient myotubes, we found decreased levels of SERCA 1 and 2 proteins, while mRNA levels remained comparable with control myotubes...
2016: Expert Reviews in Molecular Medicine
https://www.readbyqxmd.com/read/27025061/the-effect-of-the-aluminum-chloride-quercetin-complex-on-ca2-mg-2-atpase-activity-and-contraction-dynamic-properties-of-muscle-tibialis-anterior-from-rana-temporaria
#10
D M Nozdrenko, O M Abramchuk, V M Soroca, N S Miroshnichenko
Combined effect of aluminum chloride and quercetin solutions on the enzymatic activity and contraction dynamics of muscle fiber bundles of the Rana temporaria m. tibialis anterior was investigated. It was shown that these complexes inhibit muscle contraction. Linear reduction of Ca2+, Mg(2+)-ATPase activity induced by all of the used concentrations of AlCl3 - quercetin was demonstrated. It was found that complex of quercetin with AlCl3 has a greater inhibitory effect on muscle contraction dynamic and causes greater reduction during all periods of stimulation in comparison to the separate effect of the investigated compounds...
November 2015: Ukrainian Biochemical Journal
https://www.readbyqxmd.com/read/26968444/1-2-dichlorobenzene-affects-the-formation-of-the-phosphoenzyme-stage-during-the-catalytic-cycle-of-the-ca-2-atpase-from-sarcoplasmic-reticulum
#11
Javier Vargas-Medrano, Jorge A Sierra-Fonseca, Luis F Plenge-Tellechea
BACKGROUND: 1,2-Dichlorobenzene (1,2-DCB) is a benzene-derived molecule with two Cl atoms that is commonly utilized in the synthesis of pesticides. 1,2-DCB can be absorbed by living creatures and its effects on naturally-occurring enzymatic systems, including the effects on Ca(2+)-ATPases, have been poorly studied. Therefore, we aimed to study the effect of 1,2-DCB on the Ca(2+)-ATPase from sarcoplasmic reticulum (SERCA), a critical regulator of intracellular Ca(2+) concentration. RESULTS: Concentrations of 0...
March 11, 2016: BMC Biochemistry
https://www.readbyqxmd.com/read/26717594/aluminum-chloride-effect-on-ca2-mg-2-atpase-activity-and-dynamic-parameters-of-skeletal-muscle-contraction
#12
D M Nozdrenko, O M Abramchuk, V M Soroca, N S Miroshnichenko
We studied enzymatic activity and measured strain-gauge contraction properties of the frog Rana temporaria m. tibialis anterior muscle fascicles during the action of aluminum chloride solution. It was shown that AlCl3 solutions did not affect the dynamic properties of skeletal muscle preparation in concentrations less than 10(-4) M Increasing the concentration of AlCl3 to 10(-2) M induce complete inhibition of muscle contraction. A linear correlation between decrease in Ca2+,Mg(2+)-ATPase activity of sarcoplasmic reticulum and the investigated concentrations range of aluminum chloride was observed...
September 2015: Ukrainian Biochemical Journal
https://www.readbyqxmd.com/read/26713852/cellular-hypertrophy-and-increased-susceptibility-to-spontaneous-calcium-release-of-rat-left-atrial-myocytes-due-to-elevated-afterload
#13
Haifei Zhang, Mark B Cannell, Shang Jin Kim, Judy J Watson, Ruth Norman, Sarah C Calaghan, Clive H Orchard, Andrew F James
Atrial remodeling due to elevated arterial pressure predisposes the heart to atrial fibrillation (AF). Although abnormal sarcoplasmic reticulum (SR) function has been associated with AF, there is little information on the effects of elevated afterload on atrial Ca2+-handling. We investigated the effects of ascending aortic banding (AoB) on Ca2+-handling in rat isolated atrial myocytes in comparison to age-matched sham-operated animals (Sham). Myocytes were either labelled for ryanodine receptor (RyR) or loaded with fluo-3-AM and imaged by confocal microscopy...
2015: PloS One
https://www.readbyqxmd.com/read/26646371/exercise-training-restores-the-cardiac-microrna-1-and-214-levels-regulating-ca2-handling-after-myocardial-infarction
#14
Stéphano Freitas Soares Melo, Valério Garrone Barauna, Vander José Neves, Tiago Fernandes, Lucienne da Silva Lara, Diego Robles Mazzotti, Edilamar Menezes Oliveira
BACKGROUND: Impaired cardiomyocyte contractility and calcium handling are hallmarks of left ventricular contractile dysfunction. Exercise training has been used as a remarkable strategy in the treatment of heart disease. The microRNA-1, which targets sodium/calcium exchanger 1 (NCX), and microRNA-214, which targets sarcoplasmic reticulum calcium ATPase-2a (Serca2a), are involved in cardiac function regulation. Thus, the aim of this study was to evaluate the effect of exercise training on cardiac microRNA-1 and -214 expression after myocardial infarction...
2015: BMC Cardiovascular Disorders
https://www.readbyqxmd.com/read/26607901/curcumin-induces-apoptosis-by-inhibiting-sarco-endoplasmic-reticulum-ca2-atpase-activity-in-ovarian-cancer-cells
#15
Jeong-Ah Seo, Boyun Kim, Danny N Dhanasekaran, Benjamin K Tsang, Yong Sang Song
Aberrant increase in the expression levels of sarco/endoplasmic reticulum calcium ATPase (SERCA), which regulates Ca(2+) homeostasis, has been observed in ovarian cancers. In this study, we demonstrated that curcumin increases cytosolic Ca(2+) concentration through inhibition of SERCA activity, causing apoptosis in ovarian cancer cells but not in normal cells, including peripheral blood mononuclear cells (PBMCs) and ovarian surface epithelial cells (OSE). Curcumin induced apoptosis in ovarian cancer cells in a concentration- and time-dependent manner...
February 1, 2016: Cancer Letters
https://www.readbyqxmd.com/read/26547965/-activity-of-ca2-mg-2-atpase-of-sarcoplasmic-reticulum-and-contraction-strength-of-the-frog-skeletal-muscles-under-the-effect-of-organophosphorus-insecticides
#16
D M Nozdrenko, L V Korchinska, V M Soroca
The results of an experimental study of organophosphorus insecticides, including pirimiphosmethyl, diazinon and chlorpyrifos caused a decline of the contraction properties in m. tibialis anterior fiber bundles of Rana temporaria, as well as sarcoplasmic reticulum Ca2+, Mg(2+)-ATPase enzymatic activity reduction are outlined in this paper. Concentration-dependent strengths response diminishing in isolated skeletal muscle fiber bundles as a result of non-cholinergic influence of organophosphorus insecticides were found...
July 2015: Ukrainian Biochemical Journal
https://www.readbyqxmd.com/read/26442589/assembly-of-a-tyr122-hydrophobic-cluster-in-sarcoplasmic-reticulum-ca2-atpase-synchronizes-ca2-affinity-reduction-and-release-with-phosphoenzyme-isomerization
#17
Kazuo Yamasaki, Takashi Daiho, Stefania Danko, Hiroshi Suzuki
The mechanism whereby events in and around the catalytic site/head of Ca(2+)-ATPase effect Ca(2+) release to the lumen from the transmembrane helices remains elusive. We developed a method to determine deoccluded bound Ca(2+) by taking advantage of its rapid occlusion upon formation of E1PCa2 and of stabilization afforded by a high concentration of Ca(2+). The assay is applicable to minute amounts of Ca(2+)-ATPase expressed in COS-1 cells. It was validated by measuring the Ca(2+) binding properties of unphosphorylated Ca(2+)-ATPase...
November 13, 2015: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26405035/identification-of-small-ankyrin-1-as-a-novel-sarco-endo-plasmic-reticulum-ca2-atpase-1-serca1-regulatory-protein-in-skeletal-muscle
#18
Patrick F Desmond, Joaquin Muriel, Michele L Markwardt, Mark A Rizzo, Robert J Bloch
Small ankyrin 1 (sAnk1) is a 17-kDa transmembrane (TM) protein that binds to the cytoskeletal protein, obscurin, and stabilizes the network sarcoplasmic reticulum in skeletal muscle. We report that sAnk1 shares homology in its TM amino acid sequence with sarcolipin, a small protein inhibitor of the sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA). Here we investigate whether sAnk1 and SERCA1 interact. Our results indicate that sAnk1 interacts specifically with SERCA1 in sarcoplasmic reticulum vesicles isolated from rabbit skeletal muscle, and in COS7 cells transfected to express these proteins...
November 13, 2015: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26390403/kinetics-on-demand-is-a-simple-mathematical-solution-that-fits-recorded-caffeine-induced-luminal-sr-ca2-changes-in-smooth-muscle-cells
#19
Norma C Perez-Rosas, Norma L Gomez-Viquez, Adan Dagnino-Acosta, Moises Santillan, Agustín Guerrero-Hernandez
The process of Ca2+ release from sarcoplasmic reticulum (SR) comprises 4 phases in smooth muscle cells. Phase 1 is characterized by a large increase of the intracellular Ca2+ concentration ([Ca2+]i) with a minimal reduction of the free luminal SR [Ca2+] ([Ca2+]FSR). Importantly, active SR Ca2+ ATPases (SERCA pumps) are necessary for phase 1 to occur. This situation cannot be explained by the standard kinetics that involves a fixed amount of luminal Ca2+ binding sites. A new mathematical model was developed that assumes an increasing SR Ca2+ buffering capacity in response to an increase of the luminal SR [Ca2+] that is called Kinetics-on-Demand (KonD) model...
2015: PloS One
https://www.readbyqxmd.com/read/26356265/role-of-na-k-2cl-cotransporter-1-in-phenylephrine-induced-rhythmic-contraction-in-the-mouse-aorta-regulation-of-na-k-2cl-cotransporter-1-by-ca2-sparks-and-kca-channels
#20
Bing Shen, Jie Fu, Jizheng Guo, Jie Zhang, Xia Wang, Xiang Pan, Meihua Chen, Yifan Zhou, Min Zhu, Juan Du
BACKGROUND/AIMS: Vasoconstrictor-induced rhythmic contraction of arteries or veins has been observed both in vivo and in vitro. Many studies have reported that gap junctions, ryanodine receptors, Na+, K+-ATPase and other factors are involved in vasoconstrictor-induced rhythmic contraction in vascular smooth muscle. However, the mechanism is still not completely understood. METHODS: We used vessel tension measurements, intracellular recordings and intracellular Cl- concentration ([Cl-]i) measurements to investigate the mechanism underlying phenylephrine (PE)-induced rhythmic contraction in the mouse aorta...
2015: Cellular Physiology and Biochemistry
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