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sarcoplasmic reticulum ca2+ atpase

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https://www.readbyqxmd.com/read/28640447/role-of-serca-pump-in-muscle-thermogenesis-and-metabolism
#1
Muthu Periasamy, Santosh Kumar Maurya, Sanjaya Kumar Sahoo, Sushant Singh, Sanjaya Kumar Sahoo, Felipe C G Reis, Naresh Chandra Bal
In muscle cells, the sarcoplasmic reticulum (SR) not only acts as a Ca2+ store, but also regulates the contractile characteristics of the muscle. Ca2+ release from the SR is the primary mechanism for activating muscle contraction and reuptake of Ca2+ by the sarcoplasmic reticulum Ca2+ ATPase (SERCA) pump causes muscle relaxation. The SERCA pump isoforms are encoded by three genes, SERCA 1, 2, and 3, which are differentially expressed in muscle and determine SR Ca2+ dynamics by affecting the rate and amount of Ca2+ uptake, thereby affecting SR store and release of Ca2+ in muscle...
June 18, 2017: Comprehensive Physiology
https://www.readbyqxmd.com/read/28570539/monitoring-er-sr-calcium-release-with-the-targeted-ca2-sensor-catcher
#2
Florence N Reddish, Cassandra L Miller, Rakshya Gorkhali, Jenny J Yang
Intracellular calcium (Ca(2+)) transients evoked by extracellular stimuli initiate a multitude of biological processes in living organisms. At the center of intracellular calcium release are the major intracellular calcium storage organelles, the endoplasmic reticulum (ER) and the more specialized sarcoplasmic reticulum (SR) in muscle cells. The dynamic release of calcium from these organelles is mediated by the ryanodine receptor (RyR) and the inositol 1,4,5-triphosphate receptor (IP3R) with refilling occurring through the sarco/endoplasmic reticulum calcium ATPase (SERCA) pump...
May 19, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28531217/store-operated-ca2-entry-supports-contractile-function-in-hearts-of-hibernators
#3
Olga V Nakipova, Alexey S Averin, Edward V Evdokimovskii, Oleg Yu Pimenov, Leonid Kosarski, Dmitriy Ignat'ev, Andrey Anufriev, Yuri M Kokoz, Santiago Reyes, Andre Terzic, Alexey E Alekseev
Hibernators have a distinctive ability to adapt to seasonal changes of body temperature in a range between 37°C and near freezing, exhibiting, among other features, a unique reversibility of cardiac contractility. The adaptation of myocardial contractility in hibernation state relies on alterations of excitation contraction coupling, which becomes less-dependent from extracellular Ca2+ entry and is predominantly controlled by Ca2+ release from sarcoplasmic reticulum, replenished by the Ca2+-ATPase (SERCA)...
2017: PloS One
https://www.readbyqxmd.com/read/28487373/interactions-between-small-ankyrin-1-and-sarcolipin-coordinately-regulate-activity-of-the-sarco-endo-plasmic-reticulum-ca-2-atpase-serca1
#4
Patrick F Desmond, Amanda Labuza, Joaquin Muriel, Michele L Markwardt, Allison E Mancini, Mark A Rizzo, Robert J Bloch
SERCA1, the sarco(endo)plasmic reticulum Ca(2+)-ATPase of skeletal muscle, is essential for muscle relaxation and maintenance of low resting Ca(2+) levels in the myoplasm. We recently reported that small ankyrin 1 (sAnk1) interacts with the sarco(endo)plasmic reticulum Ca(2+)-ATPase in skeletal muscle (SERCA1) to inhibit its activity. We also showed that this interaction is mediated at least in part through sAnk1's transmembrane domain in a manner similar to that of sarcolipin (SLN). Earlier studies have shown that SLN and phospholamban, the other well studied small SERCA-regulatory proteins, oligomerize either alone or together...
June 30, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28472095/oxidative-profiling-of-the-failing-right-heart-in-rats-with-pulmonary-hypertension
#5
Xinhong Wang, Nataliia V Shults, Yuichiro J Suzuki
Right heart failure is the major cause of death among patients with pulmonary arterial hypertension (PAH). Understanding the biology of the right ventricle (RV) should help developing new therapeutic strategies. Rats subjected to the injection of Sugen5416 (an inhibitors of vascular endothelial growth factor receptor) plus the ovalbumin immunization had increased pulmonary arterial pressure and severe vascular remodeling. RVs of these rats were hypertrophied and had severe cardiac fibrosis. No apoptosis was, however, detected...
2017: PloS One
https://www.readbyqxmd.com/read/28406633/probing-the-conformationally-excited-states-of-membrane-proteins-via-1-h-detected-mas-solid-state-nmr-spectroscopy
#6
T Gopinath, Sarah E D Nelson, Kailey J Soller, Gianluigi Veglia
Proteins exist in ensembles of conformational states that interconvert on various motional time scales. High-energy states of proteins, often referred to as conformationally excited states, are sparsely populated and have been found to play an essential role in many biological functions. However, detecting these states is quite difficult for conventional structural techniques. Recent progress in solution NMR spectroscopy made it possible to detect conformationally excited states in soluble proteins and characterize them at high resolution...
May 4, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28398363/-gene-therapy-in-heart-failure-the-unexpected-results-from-the-cupid-2-trial
#7
Marta Gigli, Gianfranco Sinagra, Luisa Mestroni
Heart failure is still a major cause of morbidity and mortality in Europe and North America. In the last three decades, gene therapy emerged as a target in the molecular mechanisms implicated in heart failure encouraging preclinical gene therapy studies in small and large animal models. Prior studies documented a decreased expression of sarcoplasmic reticulum Ca2+-ATPase protein (SERCA2a), a major cardiac calcium cycling protein, in heart failure. These results paved the way to preliminary studies based on gene transfer strategies of SERCA2a...
February 2017: Giornale Italiano di Cardiologia
https://www.readbyqxmd.com/read/28336810/role-of-transient-receptor-potential-vanilloid-1-in-the-modulation-of-airway-smooth-muscle-tone-and-calcium-handling
#8
Gene T Yocum, Jun Chen, Christine H Choi, Elizabeth A Townsend, Yi Zhang, Dingbang Xu, Xiao W Fu, Michael J Sanderson, Charles W Emala
Asthma is a common disorder characterized, in part, by airway smooth muscle (ASM) hyperresponsiveness. Transient receptor potential vanilloid 1 (TRPV1) is a nonselective cation channel expressed on airway nerve fibers that modulates afferent signals, resulting in cough, and potentially bronchoconstriction. In the present study, the TRPV1 transcript was detected by RT-PCR in primary cultured human ASM cells, and the TRPV1 protein was detected in ASM of human trachea by immunohistochemistry. Proximity ligation assays suggest that TRPV1 is expressed in the sarcoplasmic reticulum membrane of human ASM cells in close association with sarco/endoplasmic reticulum Ca(2+)-ATPase-2...
June 1, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28273177/acute-exhaustive-aerobic-exercise-training-impair-cardiomyocyte-function-and-calcium-handling-in-sprague-dawley-rats
#9
Kristine Ljones, Henning Ofstad Ness, Karin Solvang-Garten, Svein Erik Gaustad, Morten Andre Høydal
INTRODUCTION: Recent data from long-distance endurance participants suggest that cardiac function is impaired after completion. Existing data further indicate that right ventricular function is more affected than left ventricular function. The cellular mechanisms underpinning cardiac deterioration are limited and therefore the aim of this study was to examine cardiomyocyte and molecular responses of the right and left ventricle to an acute bout of exhaustive endurance exercise. MATERIALS AND METHODS: Male Sprague-Dawley rats were assigned to sedentary controls or acute exhaustive endurance exercise consisting of a 120 minutes long forced treadmill run...
2017: PloS One
https://www.readbyqxmd.com/read/28222421/luteolin-enhances-sarcoplasmic-reticulum-ca2-atpase-activity-through-p38-mapk-signaling-thus-improving-rat-cardiac-function-after-ischemia-reperfusion
#10
Shasha Zhu, Tongda Xu, Yuanyuan Luo, Yingying Zhang, Haochen Xuan, Yanfeng Ma, Defeng Pan, Dongye Li, Hong Zhu
BACKGROUND/AIMS: A major challenge for current therapeutic strategies against ischemia/reperfusion (I/R) is the lack of effective drugs. Considering luteolin enhances the activity of sarcoplasmic reticulum Ca2+-ATPase (SERCA2a) to improve the systolic/diastolic function of rat hearts and cardiomyocytes during the I/R process, we studied the regulatory function of the p38 MAPK pathway in this protective mechanism. METHODS: Isolated cardiomyocytes and perfused hearts were separately divided into five groups and used to investigate I/R...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28218221/gene-expressions-underlying-mishandled-calcium-clearance-and-elevated-generation-of-reactive-oxygen-species-in-the-coronary-artery-smooth-muscle-cells-of-chronic-heart-failure-rats
#11
Liang Ding, Xian-Xiu Su, Wen-Hui Zhang, Yu-Xiang Xu, Xue-Feng Pan
BACKGROUND: The calcium clearance and reactive oxygen species (ROS) generations in the coronary artery smooth muscle cells in chronic heart failure (HF) have not been fully investigated. Therefore, we attempted to understand the gene expressions underlying the mishandling of calcium clearance and the accumulations of ROS. METHODS: We initially established an animal model of chronic HF by making the left anterior descending coronary artery ligation (CAL) in rats, and then isolated the coronary artery vascular smooth muscle cells from the ischemic and the nonischemic parts of the coronary artery vessels in 12 weeks after CAL operation...
February 20, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28130337/silica-nanoparticles-induce-cardiotoxicity-interfering-with-energetic-status-and-ca-2-handling-in-adult-rat-cardiomyocytes
#12
Carlos Enrique Guerrero-Beltrán, Judith Bernal-Ramírez, Omar Lozano, Yuriana Oropeza-Almazán, Elena Cristina Castillo, Jesús Roberto Garza, Noemí García, Jorge Vela, Alejandra García-García, Eduardo Ortega, Guillermo Torre-Amione, Nancy Ornelas-Soto, Gerardo García-Rivas
Recent evidence has shown that nanoparticles that have been used to improve or create new functional properties for common products may pose potential risks to human health. Silicon dioxide (SiO2) has emerged as a promising therapy vector for the heart. However, its potential toxicity and mechanisms of damage remain poorly understood. This study provides the first exploration of SiO2-induced toxicity in cultured cardiomyocytes exposed to 7- or 670-nm SiO2 particles. We evaluated the mechanism of cell death in isolated adult cardiomyocytes exposed to 24-h incubation...
April 1, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/27992596/phospholamban-ablation-using-crispr-cas9-system-improves-mortality-in-a-murine-heart-failure-model
#13
Manami Kaneko, Kentarou Hashikami, Satoshi Yamamoto, Hirokazu Matsumoto, Tomoyuki Nishimoto
Sarcoplasmic reticulum Ca2+-ATPase 2a (SERCA2a) and its inhibitory protein called phospholamban (PLN) are pivotal for Ca2+ handling in cardiomyocyte and are known that their expression level and activity were changed in the heart failure patients. To examine whether PLN inhibition can improve survival rate as well as cardiac function in heart failure, we performed PLN ablation in calsequestrin overexpressing (CSQ-Tg) mice, a severe heart failure model, using clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system...
2016: PloS One
https://www.readbyqxmd.com/read/27979982/role-of-calpain-in-eccentric-contraction-induced-proteolysis-of-ca2-regulatory-proteins-and-force-depression-in-rat-fast-twitch-skeletal-muscle
#14
Keita Kanzaki, Daiki Watanabe, Mai Kuratani, Takashi Yamada, Satoshi Matsunaga, Masanobu Wada
The aim of this study was to examine the in vivo effects of eccentric contraction (ECC) on calpain-dependent proteolysis of Ca(2+)-regulatory proteins and force production in fast-twitch skeletal muscles. Rat extensor digitorum longus muscles were exposed to 200-repeated ECC in situ and excised immediately (recovery 0) or 3 days (recovery 3) after cessation of ECC. Calpain inhibitor (CI)-treated rats were intraperitoneally injected with MDL-28170 before ECC and during 3 days of recovery. Tetanic force was markedly reduced at recovery 0 and remained reduced at recovery 3...
December 15, 2016: Journal of Applied Physiology
https://www.readbyqxmd.com/read/27737323/thyroxine-increases-serca2-and-ryr2-gene-expression-in-heart-failure-rats-with-euthyroid-sick-syndrome
#15
Fábio V G Campanha, Denise Perone, Dijon H S de Campos, Renata de A M Luvizotto, Maria T De Síbio, Miriane de Oliveira, Regiane M C Olimpio, Fernanda C F Moretto, Carlos R Padovani, Gláucia M F S Mazeto, Antonio C Cicogna, Célia R Nogueira
Objective: The current study was aimed at analyzing sarcoplasmic reticulum Ca2+ ATPase (Serca2) and ryanodine receptor type 2 (Ryr2) gene expression in rats subjected to surgery that induced HF and were subsequently treated with T4 using physiological doses. Materials and methods: HF was induced in 18 male Wistar rats by clipping the ascending thoracic aorta to generate aortic stenosis (HFS group), while the control group (9-sham) underwent thoracotomy. After 21 weeks, the HFS group was subdivided into two subgroups...
November 2016: Archives of Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27641420/gastrodin-pretreatment-impact-on-sarcoplasmic-reticulum-calcium-transport-atpase-serca-and-calcium-phosphate-plb-expression-in-rats-with-myocardial-ischemia-reperfusion
#16
Yufen Li, Xiaomei Wang, Changli Lou
BACKGROUND Calcium overload, inflammation, and apoptosis play important roles in myocardial ischemia-reperfusion injury (MIRI). Gastrodin pretreatment can alleviate MIRI. This study observed sarcoplasmic reticulum calcium transport ATPase (Ca2+-ATPase, SERCA) and calcium phosphate (PLB) protein expression in the ventricular remodeling process after myocardial infarction to explore the effect of gastrodin pretreatment on MIRI. MATERIAL AND METHODS Healthy 7-week-old male SD rats were randomly divided into a sham group (A), a model group (B), and gastrodin pretreatment groups C, D, and E (100, 200, and 400 mg/kg, respectively) with 20 in each group...
September 19, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/27591219/resveratrol-protects-against-lipopolysaccharide-induced-cardiac-dysfunction-by-enhancing-serca2a-activity-through-promoting-the-phospholamban-oligomerization
#17
Tao Bai, Xinyue Hu, Yang Zheng, Shudong Wang, Jian Kong, Lu Cai
The bacterial endotoxin lipopolysaccharide (LPS) is a main culprit responsible for cardiac dysfunction in sepsis. This study examined whether resveratrol could protect against LPS-induced cardiac dysfunction by improving the sarcoplasmic endoplasmic reticulum Ca(2+)-ATPase (SERCA2a) activity. Echocardiographic parameters, cardiomyocyte contractile and Ca(2+) transient properties, markers for cardiac inflammation, cell death, and oxidative stress, SERCA2a activity, and the ratios of phospholamban (PLB) monomer to oligomer were measured...
October 1, 2016: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/27533258/jabuticaba-induced-endothelium-independent-vasodilating-effect-on-isolated-arteries
#18
Daniela Medeiros Lobo de Andrade, Leonardo Luis Borges, Ieda Maria Sapateiro Torres, Edemilson Cardoso da Conceição, Matheus Lavorenti Rocha
Background: Despite the important biological effects of jabuticaba, its actions on the cardiovascular system have not been clarified. Objectives: To determine the effects of jabuticaba hydroalcoholic extract (JHE) on vascular smooth muscle (VSM) of isolated arteries. Methods: Endothelium-denuded aortic rings of rats were mounted in isolated organ bath to record isometric tension. The relaxant effect of JHE and the influence of K+ channels and Ca2+ intra- and extracellular sources on JHE-stimulated response were assessed...
September 2016: Arquivos Brasileiros de Cardiologia
https://www.readbyqxmd.com/read/27501399/assessment-of-myofilament-ca2-sensitivity-underlying-cardiac-excitation-contraction-coupling
#19
Zai Hao Zhao, Chun Li Jin, Ji Hyun Jang, Yu Na Wu, Sung Joon Kim, Hong Hua Jin, Lan Cui, Yin Hua Zhang
Heart failure and cardiac arrhythmias are the leading causes of mortality and morbidity worldwide. However, the mechanism of pathogenesis and myocardial malfunction in the diseased heart remains to be fully clarified. Recent compelling evidence demonstrates that changes in the myofilament Ca(2+) sensitivity affect intracellular Ca(2+) homeostasis and ion channel activities in cardiac myocytes, the essential mechanisms responsible for the cardiac action potential and contraction in healthy and diseased hearts...
2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27269924/-effects-of-hydrogen-sulfide-on-contraction-capacity-of-diaphragm-from-type-1-diabetic-rats
#20
Qiang Jia, Shanfeng Ma, Xiaofen Liu, Sai Li, Yuanyuan Wang, Qin Gao, Rui Yang
OBJECTIVE: To investigate the effects of hydrogen sulfide (H2S) on contraction capacity of diaphragm in type 1 diabetic rats.
 METHODS: Thirty-two male SD rats were randomly divided into a normal group (NC), a diabetic group (DM), a NaHS treatment group (DM+NaHS) and a NaHS group (NaHS) (n=8). Intraperitoneal injection of streptozotocin was utilized to establish diabetic rat model. After the modeling, the rats in the DM+NaHS and the NaHS groups were intraperitoneally injected with 28 μmol/kg NaHS solution...
May 2016: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
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