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sarcoplasmic reticulum ca2+ atpase

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https://www.readbyqxmd.com/read/28273177/acute-exhaustive-aerobic-exercise-training-impair-cardiomyocyte-function-and-calcium-handling-in-sprague-dawley-rats
#1
Kristine Ljones, Henning Ofstad Ness, Karin Solvang-Garten, Svein Erik Gaustad, Morten Andre Høydal
INTRODUCTION: Recent data from long-distance endurance participants suggest that cardiac function is impaired after completion. Existing data further indicate that right ventricular function is more affected than left ventricular function. The cellular mechanisms underpinning cardiac deterioration are limited and therefore the aim of this study was to examine cardiomyocyte and molecular responses of the right and left ventricle to an acute bout of exhaustive endurance exercise. MATERIALS AND METHODS: Male Sprague-Dawley rats were assigned to sedentary controls or acute exhaustive endurance exercise consisting of a 120 minutes long forced treadmill run...
2017: PloS One
https://www.readbyqxmd.com/read/28222421/luteolin-enhances-sarcoplasmic-reticulum-ca2-atpase-activity-through-p38-mapk-signaling-thus-improving-rat-cardiac-function-after-ischemia-reperfusion
#2
Shasha Zhu, Tongda Xu, Yuanyuan Luo, Yingying Zhang, Haochen Xuan, Yanfeng Ma, Defeng Pan, Dongye Li, Hong Zhu
BACKGROUND/AIMS: A major challenge for current therapeutic strategies against ischemia/reperfusion (I/R) is the lack of effective drugs. Considering luteolin enhances the activity of sarcoplasmic reticulum Ca2+-ATPase (SERCA2a) to improve the systolic/diastolic function of rat hearts and cardiomyocytes during the I/R process, we studied the regulatory function of the p38 MAPK pathway in this protective mechanism. METHODS: Isolated cardiomyocytes and perfused hearts were separately divided into five groups and used to investigate I/R...
February 21, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28218221/gene-expressions-underlying-mishandled-calcium-clearance-and-elevated-generation-of-reactive-oxygen-species-in-the-coronary-artery-smooth-muscle-cells-of-chronic-heart-failure-rats
#3
Liang Ding, Xian-Xiu Su, Wen-Hui Zhang, Yu-Xiang Xu, Xue-Feng Pan
BACKGROUND: The calcium clearance and reactive oxygen species (ROS) generations in the coronary artery smooth muscle cells in chronic heart failure (HF) have not been fully investigated. Therefore, we attempted to understand the gene expressions underlying the mishandling of calcium clearance and the accumulations of ROS. METHODS: We initially established an animal model of chronic HF by making the left anterior descending coronary artery ligation (CAL) in rats, and then isolated the coronary artery vascular smooth muscle cells from the ischemic and the nonischemic parts of the coronary artery vessels in 12 weeks after CAL operation...
February 20, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28130337/silica-nanoparticles-induce-cardiotoxicity-interfering-with-energetic-status-and-ca2-handling-in-adult-rat-cardiomyocytes
#4
Carlos Enrique Guerrero-Beltrán, Judith Bernal-Ramírez, Omar Lozano, Yuriana Oropeza-Almazán, Elena C Castillo, Jesús Roberto Garza, Noemí García, Jorge Vela, Alejandra García-García, Eduardo Ortega, Guillermo Torre-Amione, Nancy Ornelas-Soto, Gerardo García-Rivas
Recent evidence has shown that nanoparticles that have been used to improve or create new functional properties for common products may pose potential risks to human health. Silicon dioxide (SiO2) has emerged as a promising therapy vector for the heart. However, its potential toxicity and mechanisms of damage remain poorly understood. This study provides the first exploration of SiO2-induced toxicity in cultured cardiomyocytes exposed to 7 nm or 670 nm SiO2 particles. We evaluated the mechanism of cell death in isolated adult cardiomyocytes exposed to 24 h incubation...
January 27, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/27992596/phospholamban-ablation-using-crispr-cas9-system-improves-mortality-in-a-murine-heart-failure-model
#5
Manami Kaneko, Kentarou Hashikami, Satoshi Yamamoto, Hirokazu Matsumoto, Tomoyuki Nishimoto
Sarcoplasmic reticulum Ca2+-ATPase 2a (SERCA2a) and its inhibitory protein called phospholamban (PLN) are pivotal for Ca2+ handling in cardiomyocyte and are known that their expression level and activity were changed in the heart failure patients. To examine whether PLN inhibition can improve survival rate as well as cardiac function in heart failure, we performed PLN ablation in calsequestrin overexpressing (CSQ-Tg) mice, a severe heart failure model, using clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system...
2016: PloS One
https://www.readbyqxmd.com/read/27979982/role-of-calpain-in-eccentric-contraction-induced-proteolysis-of-ca2-regulatory-proteins-and-force-depression-in-rat-fast-twitch-skeletal-muscle
#6
Keita Kanzaki, Daiki Watanabe, Mai Kuratani, Takashi Yamada, Satoshi Matsunaga, Masanobu Wada
The aim of this study was to examine the in vivo effects of eccentric contraction (ECC) on calpain-dependent proteolysis of Ca(2+)-regulatory proteins and force production in fast-twitch skeletal muscles. Rat extensor digitorum longus muscles were exposed to 200-repeated ECC in situ and excised immediately (recovery 0) or 3 days (recovery 3) after cessation of ECC. Calpain inhibitor (CI)-treated rats were intraperitoneally injected with MDL-28170 before ECC and during 3 days of recovery. Tetanic force was markedly reduced at recovery 0 and remained reduced at recovery 3...
December 15, 2016: Journal of Applied Physiology
https://www.readbyqxmd.com/read/27737323/thyroxine-increases-serca2-and-ryr2-gene-expression-in-heart-failure-rats-with-euthyroid-sick-syndrome
#7
Fábio V G Campanha, Denise Perone, Dijon H S de Campos, Renata de A M Luvizotto, Maria T De Síbio, Miriane de Oliveira, Regiane M C Olimpio, Fernanda C F Moretto, Carlos R Padovani, Gláucia M F S Mazeto, Antonio C Cicogna, Célia R Nogueira
Objective: The current study was aimed at analyzing sarcoplasmic reticulum Ca2+ ATPase (Serca2) and ryanodine receptor type 2 (Ryr2) gene expression in rats subjected to surgery that induced HF and were subsequently treated with T4 using physiological doses. Materials and methods: HF was induced in 18 male Wistar rats by clipping the ascending thoracic aorta to generate aortic stenosis (HFS group), while the control group (9-sham) underwent thoracotomy. After 21 weeks, the HFS group was subdivided into two subgroups...
November 2016: Archives of Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27641420/gastrodin-pretreatment-impact-on-sarcoplasmic-reticulum-calcium-transport-atpase-serca-and-calcium-phosphate-plb-expression-in-rats-with-myocardial-ischemia-reperfusion
#8
Yufen Li, Xiaomei Wang, Changli Lou
BACKGROUND Calcium overload, inflammation, and apoptosis play important roles in myocardial ischemia-reperfusion injury (MIRI). Gastrodin pretreatment can alleviate MIRI. This study observed sarcoplasmic reticulum calcium transport ATPase (Ca2+-ATPase, SERCA) and calcium phosphate (PLB) protein expression in the ventricular remodeling process after myocardial infarction to explore the effect of gastrodin pretreatment on MIRI. MATERIAL AND METHODS Healthy 7-week-old male SD rats were randomly divided into a sham group (A), a model group (B), and gastrodin pretreatment groups C, D, and E (100, 200, and 400 mg/kg, respectively) with 20 in each group...
September 19, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/27591219/resveratrol-protects-against-lipopolysaccharide-induced-cardiac-dysfunction-by-enhancing-serca2a-activity-through-promoting-the-phospholamban-oligomerization
#9
Tao Bai, Xinyue Hu, Yang Zheng, Shudong Wang, Jian Kong, Lu Cai
The bacterial endotoxin lipopolysaccharide (LPS) is a main culprit responsible for cardiac dysfunction in sepsis. This study examined whether resveratrol could protect against LPS-induced cardiac dysfunction by improving the sarcoplasmic endoplasmic reticulum Ca(2+)-ATPase (SERCA2a) activity. Echocardiographic parameters, cardiomyocyte contractile and Ca(2+) transient properties, markers for cardiac inflammation, cell death, and oxidative stress, SERCA2a activity, and the ratios of phospholamban (PLB) monomer to oligomer were measured...
October 1, 2016: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/27533258/jabuticaba-induced-endothelium-independent-vasodilating-effect-on-isolated-arteries
#10
Daniela Medeiros Lobo de Andrade, Leonardo Luis Borges, Ieda Maria Sapateiro Torres, Edemilson Cardoso da Conceição, Matheus Lavorenti Rocha
Background: Despite the important biological effects of jabuticaba, its actions on the cardiovascular system have not been clarified. Objectives: To determine the effects of jabuticaba hydroalcoholic extract (JHE) on vascular smooth muscle (VSM) of isolated arteries. Methods: Endothelium-denuded aortic rings of rats were mounted in isolated organ bath to record isometric tension. The relaxant effect of JHE and the influence of K+ channels and Ca2+ intra- and extracellular sources on JHE-stimulated response were assessed...
September 2016: Arquivos Brasileiros de Cardiologia
https://www.readbyqxmd.com/read/27501399/assessment-of-myofilament-ca2-sensitivity-underlying-cardiac-excitation-contraction-coupling
#11
Zai Hao Zhao, Chun Li Jin, Ji Hyun Jang, Yu Na Wu, Sung Joon Kim, Hong Hua Jin, Lan Cui, Yin Hua Zhang
Heart failure and cardiac arrhythmias are the leading causes of mortality and morbidity worldwide. However, the mechanism of pathogenesis and myocardial malfunction in the diseased heart remains to be fully clarified. Recent compelling evidence demonstrates that changes in the myofilament Ca(2+) sensitivity affect intracellular Ca(2+) homeostasis and ion channel activities in cardiac myocytes, the essential mechanisms responsible for the cardiac action potential and contraction in healthy and diseased hearts...
2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27269924/-effects-of-hydrogen-sulfide-on-contraction-capacity-of-diaphragm-from-type-1-diabetic-rats
#12
Qiang Jia, Shanfeng Ma, Xiaofen Liu, Sai Li, Yuanyuan Wang, Qin Gao, Rui Yang
OBJECTIVE: To investigate the effects of hydrogen sulfide (H2S) on contraction capacity of diaphragm in type 1 diabetic rats.
 METHODS: Thirty-two male SD rats were randomly divided into a normal group (NC), a diabetic group (DM), a NaHS treatment group (DM+NaHS) and a NaHS group (NaHS) (n=8). Intraperitoneal injection of streptozotocin was utilized to establish diabetic rat model. After the modeling, the rats in the DM+NaHS and the NaHS groups were intraperitoneally injected with 28 μmol/kg NaHS solution...
May 2016: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/27235390/k201-jtv519-is-a-ca2-dependent-blocker-of-serca-and-a-partial-agonist-of-ryanodine-receptors-in-striated-muscle
#13
Yuanzhao L Darcy, Paula L Diaz-Sylvester, Julio A Copello
K201 (JTV-519) may prevent abnormal Ca(2+) leak from the sarcoplasmic reticulum (SR) in the ischemic heart and skeletal muscle (SkM) by stabilizing the ryanodine receptors (RyRs; RyR1 and RyR2, respectively). We tested direct modulation of the SR Ca(2+)-stimulated ATPase (SERCA) and RyRs by K201. In isolated cardiac and SkM SR microsomes, K201 slowed the rate of SR Ca(2+) loading, suggesting potential SERCA block and/or RyR agonism. K201 displayed Ca(2+)-dependent inhibition of SERCA-dependent ATPase activity, which was measured in microsomes incubated with 200, 2, and 0...
August 2016: Molecular Pharmacology
https://www.readbyqxmd.com/read/27174327/the-involvement-of-ca-2-signal-pathways-in-distal-colonic-myocytes-in-a-rat-model-of-dextran-sulfate-sodium-induced-colitis
#14
Yan Wang, Jun-Xia Li, Guang-Ju Ji, Kui Zhai, Hua-Hong Wang, Xin-Guang Liu
BACKGROUND: Disrupted Ca2+ homeostasis contributes to the development of colonic dysmotility in ulcerative colitis (UC), but the underlying mechanisms are unknown. This study aimed to examine the alteration of colonic smooth muscle (SM) Ca2+ signaling and Ca2+ handling proteins in a rat model of dextran sulfate sodium (DSS)-induced UC. METHODS: Male Sprague-Dawley rats were randomly divided into control (n = 18) and DSS (n = 17) groups. Acute colitis was induced by 5% DSS in the drinking water for 7 days...
May 20, 2016: Chinese Medical Journal
https://www.readbyqxmd.com/read/27055500/calpain-3-deficiency-affects-serca-expression-and-function-in-the-skeletal-muscle
#15
Ivan Toral-Ojeda, Garazi Aldanondo, Jaione Lasa-Elgarresta, Haizpea Lasa-Fernández, Roberto Fernández-Torrón, Adolfo López de Munain, Ainara Vallejo-Illarramendi
Limb-girdle muscular dystrophy type 2A (LGMD2A) is a form of muscular dystrophy caused by mutations in calpain 3 (CAPN3). Several studies have implicated Ca2+ dysregulation as an underlying event in several muscular dystrophies, including LGMD2A. In this study we used mouse and human myotube cultures, and muscle biopsies in order to determine whether dysfunction of sarco/endoplasmatic Ca2+-ATPase (SERCA) is involved in the pathology of this disease. In CAPN3-deficient myotubes, we found decreased levels of SERCA 1 and 2 proteins, while mRNA levels remained comparable with control myotubes...
April 8, 2016: Expert Reviews in Molecular Medicine
https://www.readbyqxmd.com/read/27025061/the-effect-of-the-aluminum-chloride-quercetin-complex-on-ca2-mg-2-atpase-activity-and-contraction-dynamic-properties-of-muscle-tibialis-anterior-from-rana-temporaria
#16
D M Nozdrenko, O M Abramchuk, V M Soroca, N S Miroshnichenko
Combined effect of aluminum chloride and quercetin solutions on the enzymatic activity and contraction dynamics of muscle fiber bundles of the Rana temporaria m. tibialis anterior was investigated. It was shown that these complexes inhibit muscle contraction. Linear reduction of Ca2+, Mg(2+)-ATPase activity induced by all of the used concentrations of AlCl3 - quercetin was demonstrated. It was found that complex of quercetin with AlCl3 has a greater inhibitory effect on muscle contraction dynamic and causes greater reduction during all periods of stimulation in comparison to the separate effect of the investigated compounds...
November 2015: Ukrainian Biochemical Journal
https://www.readbyqxmd.com/read/26968444/1-2-dichlorobenzene-affects-the-formation-of-the-phosphoenzyme-stage-during-the-catalytic-cycle-of-the-ca-2-atpase-from-sarcoplasmic-reticulum
#17
Javier Vargas-Medrano, Jorge A Sierra-Fonseca, Luis F Plenge-Tellechea
BACKGROUND: 1,2-Dichlorobenzene (1,2-DCB) is a benzene-derived molecule with two Cl atoms that is commonly utilized in the synthesis of pesticides. 1,2-DCB can be absorbed by living creatures and its effects on naturally-occurring enzymatic systems, including the effects on Ca(2+)-ATPases, have been poorly studied. Therefore, we aimed to study the effect of 1,2-DCB on the Ca(2+)-ATPase from sarcoplasmic reticulum (SERCA), a critical regulator of intracellular Ca(2+) concentration. RESULTS: Concentrations of 0...
March 11, 2016: BMC Biochemistry
https://www.readbyqxmd.com/read/26717594/aluminum-chloride-effect-on-ca2-mg-2-atpase-activity-and-dynamic-parameters-of-skeletal-muscle-contraction
#18
D M Nozdrenko, O M Abramchuk, V M Soroca, N S Miroshnichenko
We studied enzymatic activity and measured strain-gauge contraction properties of the frog Rana temporaria m. tibialis anterior muscle fascicles during the action of aluminum chloride solution. It was shown that AlCl3 solutions did not affect the dynamic properties of skeletal muscle preparation in concentrations less than 10(-4) M Increasing the concentration of AlCl3 to 10(-2) M induce complete inhibition of muscle contraction. A linear correlation between decrease in Ca2+,Mg(2+)-ATPase activity of sarcoplasmic reticulum and the investigated concentrations range of aluminum chloride was observed...
September 2015: Ukrainian Biochemical Journal
https://www.readbyqxmd.com/read/26713852/cellular-hypertrophy-and-increased-susceptibility-to-spontaneous-calcium-release-of-rat-left-atrial-myocytes-due-to-elevated-afterload
#19
Haifei Zhang, Mark B Cannell, Shang Jin Kim, Judy J Watson, Ruth Norman, Sarah C Calaghan, Clive H Orchard, Andrew F James
Atrial remodeling due to elevated arterial pressure predisposes the heart to atrial fibrillation (AF). Although abnormal sarcoplasmic reticulum (SR) function has been associated with AF, there is little information on the effects of elevated afterload on atrial Ca2+-handling. We investigated the effects of ascending aortic banding (AoB) on Ca2+-handling in rat isolated atrial myocytes in comparison to age-matched sham-operated animals (Sham). Myocytes were either labelled for ryanodine receptor (RyR) or loaded with fluo-3-AM and imaged by confocal microscopy...
2015: PloS One
https://www.readbyqxmd.com/read/26646371/exercise-training-restores-the-cardiac-microrna-1-and-214-levels-regulating-ca2-handling-after-myocardial-infarction
#20
Stéphano Freitas Soares Melo, Valério Garrone Barauna, Vander José Neves, Tiago Fernandes, Lucienne da Silva Lara, Diego Robles Mazzotti, Edilamar Menezes Oliveira
BACKGROUND: Impaired cardiomyocyte contractility and calcium handling are hallmarks of left ventricular contractile dysfunction. Exercise training has been used as a remarkable strategy in the treatment of heart disease. The microRNA-1, which targets sodium/calcium exchanger 1 (NCX), and microRNA-214, which targets sarcoplasmic reticulum calcium ATPase-2a (Serca2a), are involved in cardiac function regulation. Thus, the aim of this study was to evaluate the effect of exercise training on cardiac microRNA-1 and -214 expression after myocardial infarction...
2015: BMC Cardiovascular Disorders
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