Mariah Bierlein, Joseph Charles, Trevor Polisuk-Balfour, Heidi Bretscher, Micaela Rice, Jacklyn Zvonar, Drake Pohl, Lindsey Winslow, Brennah Wasie, Sara Deurloo, Jordan Van Wert, Britney Williams, Gabrielle Ankney, Zachary Harmon, Erica Dann, Anna Azuz, Alex Guzman-Vargas, Elizabeth Kuhns, Thomas P Neufeld, Michael B O'Connor, Felix Amissah, Changqi C Zhu
Autophagy, an autophagosome and lysosome-based eukaryotic cellular degradation system, has previously been implicated in lifespan regulation in different animal models. In this report, we show that expression of the RNAi transgenes targeting the transcripts of the key autophagy genes Atg1 or Atg18 in adult fly muscle or glia does not affect the overall levels of autophagosomes in those tissues and does not change the lifespan of the tested flies, but lifespan reduction phenotype has become apparent when Atg1 RNAi or Atg18 RNAi is expressed ubiquitously in adult flies or after autophagy is eradicated through the knockdown of Atg1 or Atg18 in adult fly adipocytes...
August 18, 2023: Genetics