keyword
MENU ▼
Read by QxMD icon Read
search

(LIGHT AND CAGED) OR (aptamer) OR (UNNATURAL AMINO ACID) OR (OPTOGENETIC) OR (DNA DECOY) OR (MORPHOLINO) •

keyword
https://www.readbyqxmd.com/read/28922504/selective-remodeling-of-glutamatergic-transmission-to-striatal-cholinergic-interneurons-after-dopamine-depletion
#1
Jose de Jesus Aceves Buendia, Lior Tiroshi, Wei-Hua Chiu, Joshua A Goldberg
The widely-held view that the pathophysiology of Parkinson's disease arises from an under-activation of the direct pathway striatal spiny neurons (dSPNs) has gained support from a recently described weakening of the glutamatergic projection from the parafascicular nucleus (PfN) to dSPNs in experimental parkinsonism. However, the impact of the remodeling of the thalamostriatal projection cannot be fully appreciated without considering its impact on cholinergic interneurons (ChIs) that themselves preferentially activate indirect pathway spiny neurons (iSPNs)...
September 18, 2017: European Journal of Neuroscience
https://www.readbyqxmd.com/read/28921494/solid-phase-synthesis-of-rna-analogs-containing-phosphorodithioate-linkages
#2
Xianbin Yang
The oligoribonucleotide phosphorodithioate (PS2-RNA) modification uses two sulfur atoms to replace two non-bridging oxygen atoms at an internucleotide phosphorodiester backbone linkage. Like a natural phosphodiester RNA backbone linkage, a PS2-modified backbone linkage is achiral at phosphorus. PS2-RNAs are highly stable to nucleases and several in vitro assays have demonstrated their biological activity. For example, PS2-RNAs silenced mRNA in vitro and bound to protein targets in the form of PS2-aptamers (thioaptamers)...
September 18, 2017: Current Protocols in Nucleic Acid Chemistry
https://www.readbyqxmd.com/read/28919851/deficient-sleep-in-mouse-models-of-fragile-x-syndrome
#3
R Michelle Saré, Lee Harkless, Merlin Levine, Anita Torossian, Carrie A Sheeler, Carolyn B Smith
In patients with fragile X syndrome (FXS), sleep problems are commonly observed but are not well characterized. In animal models of FXS (dfmr1 and Fmr1 knockout (KO)/Fxr2 heterozygote) circadian rhythmicity is affected, but sleep per se has not been examined. We used a home-cage monitoring system to assess total sleep time in both light and dark phases in Fmr1 KO mice at different developmental stages. Fmr1 KOs at P21 do not differ from controls, but genotype × phase interactions in both adult (P70 and P180) groups are statistically significant indicating that sleep in Fmr1 KOs is reduced selectively in the light phase compared to controls...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28919131/optogenetic-targeting-of-cardiac-myocytes-and-non-myocytes-tools-challenges-and-utility
#4
Callum M Johnston, Eva A Rog-Zielinska, Eike M Wülfers, Torsten Houwaart, Urszula Siedlecka, Thomas Knöpfel, Peter Kohl, Franziska Schneider-Warme
In optogenetics, light-activated proteins are used to monitor and modulate cellular behaviour with light. Combining genetic targeting of distinct cellular populations with defined patterns of optical stimulation enables one to study specific cell classes in complex biological tissues. In the current study we attempted to investigate the functional relevance of heterocellular electrotonic coupling in cardiac tissue in situ. In order to do that, we used a Cre-Lox approach to express the light-gated cation channel Channelrhodopsin-2 (ChR2) specifically in either cardiac myocytes or non-myocytes...
September 15, 2017: Progress in Biophysics and Molecular Biology
https://www.readbyqxmd.com/read/28918572/spot-light-on-skeletal-muscles-optogenetic-stimulation-to-understand-and-restore-skeletal-muscle-function
#5
Tobias van Bremen, Thorsten Send, Philipp Sasse, Tobias Bruegmann
Damage of peripheral nerves results in paralysis of skeletal muscle. Currently, the only treatment option to restore proper function is electrical stimulation of the innervating nerve or of the skeletal muscles directly. However this approach has low spatial and temporal precision leading to co-activation of antagonistic muscles and lacks cell-type selectivity resulting in pain or discomfort by stimulation of sensible nerves. In contrast to electrical stimulation, optogenetic methods enable spatially confined and cell-type selective stimulation of cells expressing the light sensitive channel Channelrhodopsin-2 with precise temporal control over the membrane potential...
September 16, 2017: Journal of Muscle Research and Cell Motility
https://www.readbyqxmd.com/read/28918528/signaling-of-the-complement-cleavage-product-anaphylatoxin-c5a-through-c5ar-cd88-contributes-to-pharmacological-hematopoietic-stem-cell-mobilization
#6
Kamila Bujko, Sylwia Rzeszotek, Kai Hoehlig, Jun Yan, Axel Vater, Mariusz Z Ratajczak
Several mechanisms have been postulated for orchestrating the mobilization of hematopoietic stem/progenitor cells (HSPCs), and we previously proposed that activation of the complement cascade plays a crucial role in the initiation and execution of the egress of HSPCs from bone marrow (BM) into peripheral blood (PB). In support of this notion, we demonstrated that mice deficient in the mannan-binding lectin (MBL) pathway, which activates the proximal part of the complement cascade, as well as mice deficient in the fifth component of the complement cascade (C5), which is part of the distal part of the complement cascade, are poor mobilizers...
September 16, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28918256/transgene-is-specifically-and-functionally-expressed-in-retinal-inhibitory-interneurons-in-the-vgat-chr2-eyfp-mouse
#7
Guo-Zhong Xu, Ling-Jie Cui, Ai-Lin Liu, Wei Zhou, Xue Gong, Yong-Mei Zhong, Xiong-Li Yang, Shi-Jun Weng
Ectopic transgene expression in the retina has been reported in various transgenic mice, indicating the importance of characterizing retinal phenotypes. We examined transgene expression in the VGAT-ChR2-EYFP mouse retina by fluorescent immunohistochemistry and electrophysiology, with special emphasis on EYFP localization in retinal neuronal subtypes identified by specific markers. Strong EYFP signals were detected in both the inner and outer plexiform layers. In addition, the ChR2-EYFP fusion protein was also expressed in somata of the great majority of inhibitory interneurons, including horizontal cells and GABAergic and glycinergic amacrine cells...
September 13, 2017: Neuroscience
https://www.readbyqxmd.com/read/28918052/a-ctla-4-antagonizing-dna-aptamer-with-antitumor-effect
#8
Bo-Tsang Huang, Wei-Yun Lai, Yi-Chung Chang, Jen-Wei Wang, Shauh-Der Yeh, Emily Pei-Ying Lin, Pan-Chyr Yang
The successful translation of cytotoxic T lymphocyte antigen-4 (CTLA-4) blockade has revolutionized the concept of cancer immunotherapy. Although monoclonal antibody therapeutics remain the mainstream in clinical practice, aptamers are synthetic oligonucleotides that encompass antibody-mimicking functions. Here, we report a novel high-affinity CTLA-4-antagonizing DNA aptamer (dissociation constant, 11.84 nM), aptCTLA-4, which was identified by cell-based SELEX and high-throughput sequencing. aptCTLA-4 is relatively stable in serum, promotes lymphocyte proliferation, and inhibits tumor growth in cell and animal models...
September 15, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28918021/in%C3%A2-vitro-selection-of-cell-internalizing-dna-aptamers-in-a-model-system-of-inflammatory-kidney-disease
#9
Glory Ranches, Melanie Lukasser, Herbert Schramek, Andreas Ploner, Taras Stasyk, Gert Mayer, Günter Mayer, Alexander Hüttenhofer
Chronic kidney disease (CKD) is a progressive pathological condition marked by a gradual loss of kidney function. Treatment of CKD is most effective when diagnosed at an early stage and patients are still asymptomatic. However, current diagnostic biomarkers (e.g., serum creatinine and urine albumin) are insufficient for prediction of the pathogenesis of the disease. To address this need, we applied a cell-SELEX (systematic evolution of ligands by exponential enrichment) approach and identified a series of DNA aptamers, which exhibit high affinity and selectivity for cytokine-stimulated cells, resembling some aspects of a CKD phenotype...
September 15, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28918019/dual-tumor-targeting-nanocarrier-system-for-sirna-delivery-based-on-prna-and-modified-chitosan
#10
Lin Li, Xiaoqin Hu, Min Zhang, Siyu Ma, Fanglin Yu, Shiqing Zhao, Nan Liu, Zhiyuan Wang, Yu Wang, Hua Guan, Xiujie Pan, Yue Gao, Yue Zhang, Yan Liu, Yang Yang, Xuemei Tang, Mingyuan Li, Cheng Liu, Zhiping Li, Xingguo Mei
Highly specific and efficient delivery of siRNA is still unsatisfactory. Herein, a dual tumor-targeting siRNA delivery system combining pRNA dimers with chitosan nanoparticles (CNPPs) was designed to improve the specificity and efficiency of siRNA delivery. In this dual delivery system, folate-conjugated and PEGylated chitosan nanoparticles encapsulating pRNA dimers were used as the first class of delivery system and would selectively deliver intact pRNA dimers near or into target cells. pRNA dimers simultaneously carrying siRNA and targeting aptamer, the second class of delivery system, would specifically deliver siRNA into the target cells via aptamer-mediated endocytosis or proper particle size...
September 15, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28917799/a-reagentless-and-reusable-electrochemical-aptamer-based-sensor-for-rapid-detection-of-ampicillin-in-complex-samples
#11
Zhi-Gang Yu, Rebecca Y Lai
We report the design and fabrication of a "signal-on" electrochemical aptamer-based (E-AB) sensor for detection of ampicillin. The signaling of the sensor is based on target binding-induced changes in the conformation and flexibility of the methylene blue-modified aptamer probe. The sensor's response is fast; signal saturation can be reached in ~ 200s. Since all the sensor components are surface-immobilized, it is regenerable and can be reused for at least three times. It has demonstrated good specificity and is capable of differentiating between ampicillin and structurally similar antibiotics such as amoxicillin...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917776/apta-nanosensors-for-detection-and-quantitative-determination-of-acetamiprid-a-pesticide-residue-in-food-and-environment
#12
REVIEW
Asma Verdian
In an effort to achieve high sensitive and selective detection of pesticide residues, numerous nanomaterial-based aptasensors are currently being developed for acetamiprid analysis. Recently, aptamers as a potent alternative of antibodies are used in biosensing platforms. There is tremendous interest in utilizing of nanomaterial as basic building blocks and signaling elements in aptasensors. The nanomaterials have the unique optical and electrical properties. The combination of nanomaterial and aptamer technology has opened a new window in pesticide residues monitoring...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917759/detection-of-aflatoxin-b1-in-food-samples-based-on-target-responsive-aptamer-cross-linked-hydrogel-using-a-handheld-ph-meter-as-readout
#13
Mengmeng Zhao, Peilong Wang, Yajuan Guo, Lixu Wang, Fang Luo, Bin Qiu, Longhua Guo, Xiaoou Su, Zhenyu Lin, Guonan Chen
Aflatoxin B1 (AFB1) can cause great threat to human health, so the development of convenient and portable device for sensitive detection of AFB1 is highly desired. The portable pH meter has the characters of facile operation, low cost, and easy availability. Therefore, in this study, we investigate the applicability of utilizing a pH meter as the readout to develop a portable sensor for AFB1. The specific detection of AFB1 is realized via the combination of AFB1-responsive aptamer-cross-linked hydrogel. Upon the addition of AFB1, AFB1 binds to its aptamer with high affinity in lieu of aptamer/DNA complex, causing the collapse of hydrogel network and results in the releasing of urease into the solution...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917752/facilely-self-assembled-magnetic-nanoparticles-aptamer-carbon-dots-nanocomposites-for-highly-sensitive-up-conversion-fluorescence-turn-on-detection-of-tetrodotoxin
#14
Hui Jin, Rijun Gui, Jie Sun, Yanfeng Wang
In this work, magnetic Fe3O4 nanoparticles (NPs) were modified with aptamer of tetrodotoxin (TTX) to form Fe3O4/aptamer complex. The self-assembling of carbon dots (CDs) and the aptamer generated Fe3O4/aptamer/CDs nanocomposites. The nanocomposites exhibited down-conversion fluorescence and up-conversion fluorescence (UCF) emissions simultaneously. When excited at a wavelength of 780nm, the UCF (peaked at 475nm) of nanocomposites increased regularly with the increase of TTX concentration [TTX]. In the [TTX] range from 0...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917743/selection-of-an-aptamer-against-muscovy-duck-parvovirus-for-highly-sensitive-rapid-visual-detection-by-label-free-aptasensor
#15
Taofeng Lu, Qin Ma, Wenzhuo Yan, Yuanzhi Wang, Yuanyuan Zhang, Lili Zhao, Hongyan Chen
Muscovy duck parvovirus (MDPV) causes high mortality and morbidity in ducks. This study investigated a novel aptamer-based, label-free aptasensor detection of MDPV. In this study, we developed an ssDNA aptamer using the filtration partition and lambda exonuclease method with an affinity-based monitor and counter-screening process. After 15 rounds of SELEX (systematic evolution of ligands by exponential enrichment), the ssDNA aptamer Apt-10, which specifically bound to MDPV with high affinity (Kd = 467nM) was successfully screened, and the aptamer was also found to be good specific to MDPV...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28916751/understanding-cry2-interactions-for-optical-control-of-intracellular-signaling
#16
Liting Duan, Jen Hope, Qunxiang Ong, Hsin-Ya Lou, Namdoo Kim, Comfrey McCarthy, Victor Acero, Michael Z Lin, Bianxiao Cui
Arabidopsis cryptochrome 2 (CRY2) can simultaneously undergo light-dependent CRY2-CRY2 homo-oligomerization and CRY2-CIB1 hetero-dimerization, both of which have been widely used to optically control intracellular processes. Applications using CRY2-CIB1 interaction desire minimal CRY2 homo-oligomerization to avoid unintended complications, while those utilizing CRY2-CRY2 interaction prefer robust homo-oligomerization. However, selecting the type of CRY2 interaction has not been possible as the molecular mechanisms underlying CRY2 interactions are unknown...
September 15, 2017: Nature Communications
https://www.readbyqxmd.com/read/28916555/first-person-seth-zimmerman
#17
(no author information available yet)
First Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Seth Zimmerman is the first author on 'Cells lay their own tracks - optogenetic Cdc42 activation stimulates fibronectin deposition supporting directed migration', published in Journal of Cell Science. Seth completed the work in this article as a PhD student under the supervision of Jim Bear and Brian Kuhlman at University of North Carolina at Chapel Hill, USA...
September 15, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28916520/rubrocerebellar-feedback-loop-isolates-the-interposed-nucleus-as-an-independent-processor-of-corollary-discharge-information-in-mice
#18
Christy S Beitzel, Brenda D Houck, Samantha M Lewis, Abigail L Person
Understanding cerebellar contributions to motor coordination requires deeper insight into how the output structures of the cerebellum, the cerebellar nuclei, integrate their inputs and influence downstream motor pathways. The magnocellular red nucleus (RNm), a brainstem premotor structure, is a major target of the interposed nucleus (IN), and has also been described in previous studies to send feedback collaterals to the cerebellum. Because such a pathway is in a key position to provide motor efferent information to the cerebellum, satisfying predictions about the use of corollary discharge in cerebellar computations, we studied it in mice of both sexes...
September 15, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28916151/dna-aptamer-generation-by-exselex-using-genetic-alphabet-expansion-with-a-mini-hairpin-dna-stabilization-method
#19
REVIEW
Ichiro Hirao, Michiko Kimoto, Kyung Hyun Lee
A novel aptamer generation method to greatly augment the affinity and stability of DNA aptamers was developed by genetic alphabet expansion combined with mini-hairpin DNA technology. The genetic alphabet expansion increases the physicochemical and structural diversities of DNA aptamers by introducing extra components, unnatural bases, as a fifth base, allowing for the enhancement of DNA aptamer affinities. Furthermore, the mini-hairpin DNA technology stabilizes DNA aptamers against nuclease digestion and thermal denaturation, by introducing an extraordinarily stable mini-hairpin DNA containing a GCGAAGC sequence...
September 12, 2017: Biochimie
https://www.readbyqxmd.com/read/28916037/electrochemical-aptasensor-for-human-osteopontin-detection-using-a-dna-aptamer-selected-by-selex
#20
Sofia G Meirinho, Luís G Dias, António M Peres, Lígia R Rodrigues
A DNA aptamer with affinity and specificity for human osteopontin (OPN), a potential breast cancer biomarker, was selected using the SELEX process, considering its homology rate and the stability of its secondary structures. This aptamer exhibited a satisfactory affinity towards OPN, showing dissociation constants lower than 2.5 nM. It was further used to develop a simple, label-free electrochemical aptasensor against OPN. The aptasensor showed good sensitivity towards OPN in standard solutions, being the square wave voltammetry (SWV), compared to the cyclic voltammetry, the most sensitive technique with detection and quantification limits of 1...
September 22, 2017: Analytica Chimica Acta
keyword
keyword
118774
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"