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5-methyltetrahydrofolate

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https://www.readbyqxmd.com/read/28475162/characterization-and-interrelations-of-one-carbon-metabolites-in-tissues-erythrocytes-and-plasma-in-mice-with-dietary-induced-folate-deficiency
#1
Markus Kopp, Rosalie Morisset, Michael Rychlik
Studies on one-carbon metabolism for the assessment of folate deficiency have focused on either metabolites of folate metabolism or methionine cycle. To bridge the gap between deficiency markers in these pathways we designed a dietary induced folate deficiency study using male C57BL/6N mice. After weaning (3 weeks) mice were fed a defined control diet (1 week) before being fed a folate deficient diet (n = 6 mice) and the control diet (n = 6 mice) for 12 additional weeks. Thereafter, we determined total homocysteine in plasma and folate in erythrocytes as well as S-adenosylmethionine, S-adenosylhomocysteine, and six folate vitamers in tissues including 5-methyltetrahydrofolate, 5-formyltetrahydrofolate, 5,10-methenyltetrahydrofolate, tetrahydrofolate, 10-formylfolic acid, and folic acid by means of stable isotope dilution assays coupled with liquid chromatography tandem mass spectrometry...
May 5, 2017: Nutrients
https://www.readbyqxmd.com/read/28461497/folate-rescues-vitamin-b12-depletion-induced-inhibition-of-nuclear-thymidylate-biosynthesis-and-genome-instability
#2
Ashley M Palmer, Elena Kamynina, Martha S Field, Patrick J Stover
Clinical vitamin B12 deficiency can result in megaloblastic anemia, which results from the inhibition of DNA synthesis by trapping folate cofactors in the form of 5-methyltetrahydrofolate (5-methylTHF) and subsequent inhibition of de novo thymidylate (dTMP) biosynthesis. In the cytosol, vitamin B12 functions in the remethylation of homocysteine to methionine, which regenerates THF from 5-methylTHF. In the nucleus, THF is required for de novo dTMP biosynthesis, but it is not understood how 5-methylTHF accumulation in the cytosol impairs nuclear dTMP biosynthesis...
May 1, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28460586/supplementation-with-6s-5-methyltetrahydrofolate-or-folic-acid-equally-reduces-serum-homocysteine-concentrations-in-older-adults
#3
Ewa Sicińska, Anna Brzozowska, Wojciech Roszkowski, Paul M Finglas
Folic acid (FA) supplementation reduces the elevated serum homocysteine (Hcy) concentrations. [6 S]-5-methyltetrahydrofolate ([6 S]-5-MTHF) is an alternative to FA due to possible advantages, that is, no masking cobalamin deficiency. The study aim was to evaluate the effectiveness of [6 S]-5-MTHF in relations to FA supplementation in reducing the serum Hcy. Healthy volunteers, aged 50-65, had normal serum folate and did not use supplements with B-vitamins for 6 months. Forty subjects were divided into two groups: receiving 400 μg/d FA or the equimolar amount of [6 S]-5-MTHF...
May 2, 2017: International Journal of Food Sciences and Nutrition
https://www.readbyqxmd.com/read/28459050/assessing-volumetric-absorptive-microsampling-coupled-with-stable-isotope-dilution-assay-and-liquid-chromatography-tandem-mass-spectrometry-as-potential-diagnostic-tool-for-whole-blood-5-methyltetrahydrofolic-acid
#4
Markus Kopp, Michael Rychlik
Volumetric absorptive microsamplers (VAMS) have been developed recently as a promising tool for clinical blood sampling. Compared to dried blood spot samples analyzed by accurate stable isotope dilution assays (SIDAs), the new technique could provide further substantial miniaturizing of folate assays by eliminating hematocrit effects and uneven analyte distribution within the sample. Herein, we present a miniaturized SIDA coupled with LC-MS/MS measurement of 5-methyltetrahydrofolic acid as main folate vitamer in whole blood (WB) using [(13)C5]-5-methyltetrahydrofolic acid as internal standard...
2017: Frontiers in Nutrition
https://www.readbyqxmd.com/read/28377750/measurements-of-intra-and-extra-cellular-5-methyltetrahydrofolate-indicate-that-bifidobacterium-adolescentis-dsm-20083-t-and-bifidobacterium-pseudocatenulatum-dsm-20438-t-do-not-actively-excrete-5-methyltetrahydrofolate-in-vitro
#5
Markus Kopp, Kerstin Dürr, Matthias Steigleder, Thomas Clavel, Michael Rychlik
Certain intestinal bifidobacteria have the ability to synthesize folates. In vitro experiments revealed a high production, cellular accumulation, and release of reduced folate vitamers like 5-methyltetrahydrofolate and tetrahydrofolate in folate-free medium (FFM). However, it is still unclear to which extent synthesized folates are polyglutamylated and probably not available for transport, and if they are actively released by excretion. To address these questions, we characterized intra- and extra-cellular pteroylmonoglutamates and polyglutamylated 5-methyltetrahydrofolate (5-CH3-H4PteGlu2-4) in Bifidobacterium adolescentis DSM 20083(T) and Bifidobacterium pseudocatenulatum DSM 20438(T)in vitro...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28367928/quantification-of-total-folate-folate-species-and-polyglutamyl-folate-distribution-in-winged-beans-psophocarus-tetragonolobus-l-dc-from-different-cultivars-and-growth-stages-by-ultra-high-performance-liquid-chromatography-tandem-mass-spectrometry
#6
Shuangyan Luo, Hanying Duan, Yuchen Zou, Ruixia Qiu, Chao Wang
Winged beans are an important natural source of some micronutrients. This paper presents the first complete characterization of folate derivatives including polyglutamyl 5-methyltetrahydrofolate (5-CH3-H4PteGlun), folate species and total folate accumulating in pods and immature seeds of winged beans from 9 cultivars and 7 growth stages. 5-CH3-H4PteGlun and folate species were determined with a UHPLC-MS/MS method. Accurate determination of 5-CH3-H4PteGlun and folate species was optimized and validated according to EMA guidelines including method selectivity, sensitivity, linearity, accuracy, precision, matrix effect and carry-over...
2017: Journal of Nutritional Science and Vitaminology
https://www.readbyqxmd.com/read/28351446/on-the-relationship-between-head-circumference-brain-size-prenatal-long-chain-pufa-5-methyltetrahydrofolate-supplementation-and-cognitive-abilities-during-childhood
#7
Andrés Catena, Cristina Martínez-Zaldívar, Carolina Diaz-Piedra, Francisco J Torres-Espínola, Pilar Brandi, Miguel Pérez-García, Tamás Decsi, Berthold Koletzko, Cristina Campoy
Head circumference in infants has been reported to predict brain size, total grey matter volume (GMV) and neurocognitive development. However, it is unknown whether it has predictive value on regional and subcortical brain volumes. We aimed to explore the relationship between several head circumference measurements since birth and distributions of GMV and subcortical volumes at later childhood. We examined seventy-four, Caucasian, singleton, term-born infants born to mothers randomised to receive fish oil and/or 5-methyltetrahydrofolate or placebo prenatal supplementation...
March 29, 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/28152052/choline-and-methionine-differentially-alter-methyl-carbon-metabolism-in-bovine-neonatal-hepatocytes
#8
Tawny L Chandler, Heather M White
Intersections in hepatic methyl group metabolism pathways highlights potential competition or compensation of methyl donors. The objective of this experiment was to examine the expression of genes related to methyl group transfer and lipid metabolism in response to increasing concentrations of choline chloride (CC) and DL-methionine (DLM) in primary neonatal hepatocytes that were or were not exposed to fatty acids (FA). Primary hepatocytes isolated from 4 neonatal Holstein calves were maintained as monolayer cultures for 24 h before treatment with CC (61, 128, 2028, and 4528 μmol/L) and DLM (16, 30, 100, 300 μmol/L), with or without a 1 mmol/L FA cocktail in a factorial arrangement...
2017: PloS One
https://www.readbyqxmd.com/read/28050625/origins-of-the-difference-between-food-folate-analysis-results-obtained-by-lc-ms-ms-and-microbiological-assays
#9
Christiane Ringling, Michael Rychlik
To investigate the often reported disagreement in food folate quantitation between the microbiological assay and high-performance liquid chromatography methods, different foods were analyzed both by a microbiological assay and by a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. For the LC-MS/MS analysis we emphasize the need for complete deconjugation of polyglutamic folate forms. Moreover, our results revealed no need for an additional enzyme treatment except in the deconjugation step. To check the efficiency of deconjugation without additional sample preparations, the amount of diglutamates was quantified and samples were screened for additional polyglutamates...
March 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28025402/author-response-csf-concentrations-of-5-methyltetrahydrofolate-in-a-cohort-of-young-children-with-autism
#10
John Shoffner
No abstract text is available yet for this article.
January 3, 2017: Neurology
https://www.readbyqxmd.com/read/28025401/letter-re-csf-concentrations-of-5-methyltetrahydrofolate-in-a-cohort-of-young-children-with-autism
#11
Geraldine Barrett
No abstract text is available yet for this article.
January 3, 2017: Neurology
https://www.readbyqxmd.com/read/27940130/simultaneous-measurement-of-monoamine-metabolites-and-5-methyltetrahydrofolate-in-the-cerebrospinal-fluid-of-children
#12
Tomoyuki Akiyama, Yumiko Hayashi, Yoshiyuki Hanaoka, Takashi Shibata, Mari Akiyama, Kazuyuki Nakamura, Yu Tsuyusaki, Masaya Kubota, Harumi Yoshinaga, Katsuhiro Kobayashi
BACKGROUND: We describe a new method for simultaneous measurement of monoamine metabolites (3-O-methyldopa [3-OMD], 3-methoxy-4-hydroxyphenylethyleneglycol [MHPG], 5-hydroxyindoleacetic acid [5-HIAA], and homovanillic acid [HVA]) and 5-methyltetrahydrofolate (5-MTHF) and its use on cerebrospinal fluid (CSF) samples from pediatric patients. METHODS: Monoamine metabolites and 5-MTHF were measured by high-performance liquid chromatography with fluorescence detection...
February 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27916687/a-unique-combination-of-micronutrients-rejuvenates-cognitive-performance-in-aged-mice
#13
Sam D Perez, Kristy Du, Catarina Rendeiro, Lin Wang, Qian Wu, Stanislav S Rubakhin, Rema Vazhappilly, Jeffrey H Baxter, Jonathan V Sweedler, Justin S Rhodes
It is widely believed that diet can influence the onset and severity of cognitive aging, but the optimal combination of micronutrients and molecular and cellular mechanisms remain elusive. The purpose of this study was to compare the effects of eight distinct diets, consisting of various concentrations of selected micronutrients, on learning and memory as well as markers of neuronal plasticity, and metabolic and neuro-immune status of the aged hippocampus. Eighteen-month-old male and female C57BL/6J mice were fed the diets for 16 weeks, followed by learning and memory trials on the active avoidance task...
December 2, 2016: Behavioural Brain Research
https://www.readbyqxmd.com/read/27896774/multiplex-measurement-of-serum-folate-vitamers-by-uplc-ms-ms
#14
Sarah Meadows
The implementation of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to measure six folate vitamers in serum samples allows a more individual and accurate measurement than the commonly used immunoassays. In the described method, serum samples undergo solid phase extraction followed by liquid chromatography coupled with electrospray ionization tandem mass spectrometry with a run time of 3.5 min. Recovery is 95 % for the most important folate metabolite, 5-methyltetrahydrofolate (MTHF), and greater than 78 % for other minor folate forms...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27881594/hepatic-activity-and-transcription-of-betaine-homocysteine-methyltransferase-methionine-synthase-and-cystathionine-synthase-in-periparturient-dairy-cows-are-altered-to-different-extents-by-supply-of-methionine-and-choline
#15
Zheng Zhou, Timothy A Garrow, Xianwen Dong, Daniel N Luchini, Juan J Loor
BACKGROUND: Compared with choline, Met enhances milk yield and feed intake, and elicits a better immuno-metabolic status in periparturient cows. It is unknown whether hepatic activity and transcription of betaine-homocysteine methyltransferase (BHMT), 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), and cystathionine β-synthase (CBS) are responsive to Met and choline supply. OBJECTIVE: This study sought to characterize hepatic BHMT, MTR, and CBS transcription and activity in response to Met and choline supplementation...
November 23, 2016: Journal of Nutrition
https://www.readbyqxmd.com/read/27860207/mediterranean-diet-gene-interactions-a-targeted-metabolomics-study-in-greek-cypriot-women
#16
Maria G Kakkoura, Kleitos Sokratous, Christiana A Demetriou, Maria A Loizidou, Giorgos Loucaides, Eleni Kakouri, Andreas Hadjisavvas, Kyriacos Kyriacou
SCOPE: A high adherence to the Mediterranean diet (MD) was previously associated with a decreased risk of breast cancer (BC) among Greek-Cypriot women. Additionally, particular polymorphisms were shown to modulate this MD-BC association. Herein, we aimed to investigate the effect of polymorphisms-MD interactions on the levels of specific metabolites that could be related to dietary adherence or enzymatic activity, which is itself modulated by polymorphisms. METHODS AND RESULTS: Greek-Cypriot women who were BC controls and had the lowest or the highest MD adherence (vegetables, fruit, legumes, fish) as assessed by principal component analysis (n = 564) were included...
November 18, 2016: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/27845713/novel-approaches-to-investigate-one-carbon-metabolism-and-related-b-vitamins-in-blood-pressure
#17
REVIEW
Amy McMahon, Helene McNulty, Catherine F Hughes, J J Strain, Mary Ward
Hypertension, a major risk factor for heart disease and stroke, is the world's leading cause of preventable, premature death. A common polymorphism (677C→T) in the gene encoding the folate metabolizing enzyme methylenetetrahydrofolate reductase (MTHFR) is associated with increased blood pressure, and there is accumulating evidence demonstrating that this phenotype can be modulated, specifically in individuals with the MTHFR 677TT genotype, by the B-vitamin riboflavin, an essential co-factor for MTHFR. The underlying mechanism that links this polymorphism, and the related gene-nutrient interaction, with hypertension is currently unknown...
November 11, 2016: Nutrients
https://www.readbyqxmd.com/read/27808252/functional-variants-of-the-5-methyltetrahydrofolate-homocysteine-methyltransferase-gene-significantly-increase-susceptibility-to-prostate-cancer-results-from-an-ethnic-han-chinese-population
#18
Yuan-Yuan Qu, Shu-Xian Zhou, Xuan Zhang, Rui Zhao, Cheng-Yuan Gu, Kun Chang, Xiao-Qun Yang, Hua-Lei Gan, Bo Dai, Hai-Liang Zhang, Guo-Hai Shi, Yao Zhu, Ding-Wei Ye, Jian-Yuan Zhao
Aberrant DNA methylation has been implicated in prostate carcinogenesis. The one-carbon metabolism pathway and related metabolites determine cellular DNA methylation and thus is thought to play a pivotal role in PCa occurrence. This study aimed to investigate the contribution of genetic variants in one-carbon metabolism genes to prostate cancer (PCa) risk and the underlying biological mechanisms. In this hospital-based case-control study of 1817 PCa cases and 2026 cancer-free controls, we genotyped six polymorphisms in three one-carbon metabolism genes and assessed their association with the risk of PCa...
November 3, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27757515/high-throughput-and-simultaneous-quantitative-analysis-of-homocysteine-methionine-cycle-metabolites-and-co-factors-in-blood-plasma-and-cerebrospinal-fluid-by-isotope-dilution-lc-ms-ms
#19
Seu Ping Guiraud, Ivan Montoliu, Laeticia Da Silva, Loïc Dayon, Antonio Núñez Galindo, John Corthésy, Martin Kussmann, Francois-Pierre Martin
The methionine cycle is a key pathway contributing to the regulation of human health, with well-established involvement in cardiovascular diseases and cognitive function. Changes in one-carbon cycle metabolites have also been associated with mild cognitive decline, vascular dementia, and Alzheimer's disease. Today, there is no single analytical method to monitor both metabolites and co-factors of the methionine cycle. To address this limitation, we here report for the first time a new method for the simultaneous quantitation of 17 metabolites in the methionine cycle, which are homocysteic acid, taurine, serine, cysteine, glycine, homocysteine, riboflavin, methionine, pyridoxine, cystathionine, pyridoxamine, S-adenosylhomocysteine, S-adenosylmethionine, betaine, choline, dimethylglycine, and 5-methyltetrahydrofolic acid...
January 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27743887/severe-leukoencephalopathy-with-cortical-involvement-and-peripheral-neuropathy-due-to-folr1-deficiency
#20
Yu Kobayashi, Jun Tohyama, Tomoyuki Akiyama, Shinichi Magara, Hideshi Kawashima, Noriyuki Akasaka, Mitsuko Nakashima, Hirotomo Saitsu, Naomichi Matsumoto
Cerebral folate deficiency due to folate receptor 1 gene (FOLR1) mutations is an autosomal recessive disorder resulting from a brain-specific folate transport defect. It is characterized by late infantile onset, severe psychomotor regression, epilepsy, and leukodystrophy. We describe a consanguineous girl exhibiting severe developmental regression, intractable epilepsy, polyneuropathy, and profound hypomyelination with cortical involvement. Magnetic resonance imaging showed cortical disturbances in addition to profound hypomyelination and cerebellar atrophy...
March 2017: Brain & Development
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